No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 52 |
| AciI | CCGC | 3 cut(s) | 296, 400, 527 |
| AcoI | YGGCCR | 4 cut(s) | 297, 336, 397, 547 |
| AfaI | GTAC | 2 cut(s) | 543, 588 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 520 |
| AgeI | ACCGGT | 1 cut(s) | 55 |
| AgsI | TTSAA | 2 cut(s) | 443, 609 |
| AhlI | ACTAGT | 1 cut(s) | 305 |
| AluBI | AGCT | 4 cut(s) | 287, 432, 475, 509 |
| AluI | AGCT | 4 cut(s) | 287, 432, 475, 509 |
| Alw26I | GTCTC | 1 cut(s) | 586 |
| Ama87I | CYCGRG | 1 cut(s) | 13 |
| AoxI | GGCC | 4 cut(s) | 297, 336, 397, 547 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 209, 241 |
| AsiGI | ACCGGT | 1 cut(s) | 55 |
| AsuC2I | CCSGG | 2 cut(s) | 14, 15 |
| AsuHPI | GGTGA | 5 cut(s) | 16, 153, 245, 275, 476 |
| AvaI | CYCGRG | 1 cut(s) | 13 |
| BanI | GGYRCC | 1 cut(s) | 52 |
| BanII | GRGCYC | 1 cut(s) | 136 |
| BbsI | GAAGAC | 2 cut(s) | 222, 537 |
| BbvCI | CCTCAGC | 1 cut(s) | 288 |
| BccI | CCATC | 1 cut(s) | 389 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 105, 139 |
| BclI | TGATCA | 1 cut(s) | 406 |
| BcnI | CCSGG | 2 cut(s) | 14, 15 |
| BcoDI | GTCTC | 1 cut(s) | 586 |
| BcuI | ACTAGT | 1 cut(s) | 305 |
| BfaI | CTAG | 2 cut(s) | 260, 306 |
| BisI | GCNGC | 2 cut(s) | 297, 400 |
| BlsI | GCNGC | 2 cut(s) | 298, 401 |
| Bme1390I | CCNGG | 2 cut(s) | 14, 15 |
| BmeT110I | CYCGRG | 1 cut(s) | 13 |
| BmiI | GGNNCC | 1 cut(s) | 54 |
| BmrFI | CCNGG | 2 cut(s) | 14, 15 |
| BmsI | GCATC | 5 cut(s) | 125, 283, 301, 427, 514 |
| BpiI | GAAGAC | 2 cut(s) | 222, 537 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 86, 118 |
| BpmI | CTGGAG | 1 cut(s) | 303 |
| Bpu10I | CCTNAGC | 2 cut(s) | 288, 519 |
| BpuMI | CCSGG | 2 cut(s) | 14, 15 |
| BsaJI | CCNNGG | 4 cut(s) | 13, 87, 435, 612 |
| BsaWI | WCCGGW | 1 cut(s) | 55 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 520 |
| Bse118I | RCCGGY | 1 cut(s) | 55 |
| Bse3DI | GCAATG | 1 cut(s) | 43 |
| BseDI | CCNNGG | 4 cut(s) | 13, 87, 435, 612 |
| BseGI | GGATG | 4 cut(s) | 298, 418, 460, 529 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 520 |
| BseMI | GCAATG | 1 cut(s) | 43 |
| BseMII | CTCAG | 2 cut(s) | 279, 519 |
| BseX3I | CGGCCG | 1 cut(s) | 297 |
| Bsh1285I | CGRYCG | 1 cut(s) | 300 |
| BshFI | GGCC | 4 cut(s) | 299, 338, 399, 549 |
| BshNI | GGYRCC | 1 cut(s) | 52 |
| BshTI | ACCGGT | 1 cut(s) | 55 |
| BsiEI | CGRYCG | 1 cut(s) | 300 |
| BsiHKCI | CYCGRG | 1 cut(s) | 13 |
| BsiSI | CCGG | 3 cut(s) | 14, 56, 194 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 520 |
| BsmAI | GTCTC | 1 cut(s) | 586 |
| BsnI | GGCC | 4 cut(s) | 299, 338, 399, 549 |
| BsoBI | CYCGRG | 1 cut(s) | 13 |
| Bsp1286I | GDGCHC | 1 cut(s) | 136 |
| Bsp143I | GATC | 2 cut(s) | 183, 406 |
| Bsp19I | CCATGG | 1 cut(s) | 435 |
| BspACI | CCGC | 3 cut(s) | 296, 400, 527 |
| BspANI | GGCC | 4 cut(s) | 299, 338, 399, 549 |
| BspCNI | CTCAG | 2 cut(s) | 280, 518 |
| BspLI | GGNNCC | 1 cut(s) | 54 |
| BspQI | GCTCTTC | 2 cut(s) | 87, 124 |
| BspT107I | GGYRCC | 1 cut(s) | 52 |
| BsrDI | GCAATG | 1 cut(s) | 43 |
| BsrFI | RCCGGY | 1 cut(s) | 55 |
| BssAI | RCCGGY | 1 cut(s) | 55 |
| BssECI | CCNNGG | 4 cut(s) | 13, 87, 435, 612 |
| BssMI | GATC | 2 cut(s) | 183, 406 |
| BssT1I | CCWWGG | 3 cut(s) | 87, 435, 612 |
| Bst6I | CTCTTC | 3 cut(s) | 87, 124, 418 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 59 |
| BstDEI | CTNAG | 3 cut(s) | 288, 505, 519 |
| BstDSI | CCRYGG | 1 cut(s) | 435 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 518 |
| BstF5I | GGATG | 4 cut(s) | 298, 418, 460, 529 |
| BstKTI | GATC | 2 cut(s) | 186, 409 |
| BstMAI | GTCTC | 1 cut(s) | 586 |
| BstMBI | GATC | 2 cut(s) | 183, 406 |
| BstMCI | CGRYCG | 1 cut(s) | 300 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 59, 293, 506, 515, 524 |
| BstSCI | CCNGG | 2 cut(s) | 12, 13 |
| BstV2I | GAAGAC | 2 cut(s) | 222, 537 |
| BstZI | CGGCCG | 1 cut(s) | 297 |
| BsuRI | GGCC | 4 cut(s) | 299, 338, 399, 549 |
| BtgI | CCRYGG | 1 cut(s) | 435 |
| BtsCI | GGATG | 4 cut(s) | 298, 418, 460, 529 |
| Cfr10I | RCCGGY | 1 cut(s) | 55 |
| Cfr9I | CCCGGG | 1 cut(s) | 13 |
| Csp6I | GTAC | 2 cut(s) | 542, 587 |
| CspAI | ACCGGT | 1 cut(s) | 55 |
| CviAII | CATG | 4 cut(s) | 277, 386, 436, 451 |
| CviQI | GTAC | 2 cut(s) | 542, 587 |
| DdeI | CTNAG | 3 cut(s) | 288, 505, 519 |
| DpnI | GATC | 2 cut(s) | 185, 408 |
| DpnII | GATC | 2 cut(s) | 183, 406 |
| EaeI | YGGCCR | 4 cut(s) | 297, 336, 397, 547 |
| EagI | CGGCCG | 1 cut(s) | 297 |
| Eam1104I | CTCTTC | 3 cut(s) | 87, 124, 418 |
| EarI | CTCTTC | 3 cut(s) | 87, 124, 418 |
| EclXI | CGGCCG | 1 cut(s) | 297 |
| Eco130I | CCWWGG | 3 cut(s) | 87, 435, 612 |
| Eco24I | GRGCYC | 1 cut(s) | 136 |
| Eco52I | CGGCCG | 1 cut(s) | 297 |
| Eco88I | CYCGRG | 1 cut(s) | 13 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 518 |
| EcoT14I | CCWWGG | 3 cut(s) | 87, 435, 612 |
| EcoT38I | GRGCYC | 1 cut(s) | 136 |
| ErhI | CCWWGG | 3 cut(s) | 87, 435, 612 |
| FaeI | CATG | 4 cut(s) | 280, 389, 439, 454 |
| FaiI | YATR | 9 cut(s) | 6, 206, 221, 239, 278, 387, 437, 452, 656 |
| FatI | CATG | 4 cut(s) | 276, 385, 435, 450 |
| FbaI | TGATCA | 1 cut(s) | 406 |
| Fnu4HI | GCNGC | 2 cut(s) | 297, 400 |
| FokI | GGATG | 4 cut(s) | 305, 405, 467, 536 |
| FriOI | GRGCYC | 1 cut(s) | 136 |
| Fsp4HI | GCNGC | 2 cut(s) | 297, 400 |
| FspBI | CTAG | 2 cut(s) | 260, 306 |
| GluI | GCNGC | 2 cut(s) | 297, 400 |
| GsuI | CTGGAG | 1 cut(s) | 303 |
| HaeIII | GGCC | 4 cut(s) | 299, 338, 399, 549 |
| HapII | CCGG | 3 cut(s) | 14, 56, 194 |
| Hin1II | CATG | 4 cut(s) | 280, 389, 439, 454 |
| HinfI | GANTC | 2 cut(s) | 9, 190 |
| HpaII | CCGG | 3 cut(s) | 14, 56, 194 |
| HphI | GGTGA | 5 cut(s) | 16, 153, 245, 275, 476 |
| Hpy166II | GTNNAC | 2 cut(s) | 24, 542 |
| Hpy188III | TCNNGA | 5 cut(s) | 126, 187, 282, 425, 660 |
| Hpy8I | GTNNAC | 2 cut(s) | 24, 542 |
| HpyAV | CCTTC | 4 cut(s) | 43, 142, 341, 660 |
| HpyCH4IV | ACGT | 1 cut(s) | 168 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 59, 293, 506, 515, 524 |
| HpyF3I | CTNAG | 3 cut(s) | 288, 505, 519 |
| HpySE526I | ACGT | 1 cut(s) | 168 |
| Hsp92II | CATG | 4 cut(s) | 280, 389, 439, 454 |
| Ksp22I | TGATCA | 1 cut(s) | 406 |
| Kzo9I | GATC | 2 cut(s) | 183, 406 |
| LguI | GCTCTTC | 2 cut(s) | 87, 124 |
| LmnI | GCTCC | 2 cut(s) | 284, 437 |
| LpnPI | CCDG | 9 cut(s) | 27, 69, 149, 207, 267, 369, 384, 438, 485 |
| LweI | GCATC | 5 cut(s) | 125, 283, 301, 427, 514 |
| MaeI | CTAG | 2 cut(s) | 260, 306 |
| MaeII | ACGT | 1 cut(s) | 168 |
| MaeIII | GTNAC | 1 cut(s) | 159 |
| MalI | GATC | 2 cut(s) | 185, 408 |
| MboI | GATC | 2 cut(s) | 183, 406 |
| MboII | GAAGA | 7 cut(s) | 58, 104, 141, 227, 405, 542, 617 |
| MhlI | GDGCHC | 1 cut(s) | 136 |
| MluCI | AATT | 3 cut(s) | 222, 444, 604 |
| MmeI | TCCRAC | 1 cut(s) | 659 |
| MnlI | CCTC | 7 cut(s) | 112, 223, 283, 325, 432, 547, 584 |
| MseI | TTAA | 1 cut(s) | 564 |
| MspI | CCGG | 3 cut(s) | 14, 56, 194 |
| MspR9I | CCNGG | 2 cut(s) | 14, 15 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 59, 293, 506, 515, 524 |
| NciI | CCSGG | 2 cut(s) | 14, 15 |
| NcoI | CCATGG | 1 cut(s) | 435 |
| NdeII | GATC | 2 cut(s) | 183, 406 |
| NlaIII | CATG | 4 cut(s) | 280, 389, 439, 454 |
| NlaIV | GGNNCC | 1 cut(s) | 54 |
| NmeAIII | GCCGAG | 1 cut(s) | 575 |
| NmuCI | GTSAC | 1 cut(s) | 159 |
| PciSI | GCTCTTC | 2 cut(s) | 87, 124 |
| PfeI | GAWTC | 2 cut(s) | 9, 190 |
| PinAI | ACCGGT | 1 cut(s) | 55 |
| PkrI | GCNGC | 2 cut(s) | 298, 401 |
| PspN4I | GGNNCC | 1 cut(s) | 54 |
| RsaI | GTAC | 2 cut(s) | 543, 588 |
| RsaNI | GTAC | 2 cut(s) | 542, 587 |
| SapI | GCTCTTC | 2 cut(s) | 87, 124 |
| SaqAI | TTAA | 1 cut(s) | 564 |
| SatI | GCNGC | 2 cut(s) | 297, 400 |
| Sau3AI | GATC | 2 cut(s) | 183, 406 |
| ScrFI | CCNGG | 2 cut(s) | 14, 15 |
| SduI | GDGCHC | 1 cut(s) | 136 |
| SfaNI | GCATC | 5 cut(s) | 125, 283, 301, 427, 514 |
| SmaI | CCCGGG | 1 cut(s) | 15 |
| SpeI | ACTAGT | 1 cut(s) | 305 |
| Sse9I | AATT | 3 cut(s) | 222, 444, 604 |
| SsiI | CCGC | 3 cut(s) | 296, 400, 527 |
| SspMI | CTAG | 2 cut(s) | 260, 306 |
| StyD4I | CCNGG | 2 cut(s) | 12, 13 |
| StyI | CCWWGG | 3 cut(s) | 87, 435, 612 |
| TaiI | ACGT | 1 cut(s) | 171 |
| TaqI | TCGA | 4 cut(s) | 68, 182, 228, 486 |
| TasI | AATT | 3 cut(s) | 222, 444, 604 |
| TatI | WGTACW | 1 cut(s) | 541 |
| TauI | GCSGC | 2 cut(s) | 299, 402 |
| TfiI | GAWTC | 2 cut(s) | 9, 190 |
| Tru1I | TTAA | 1 cut(s) | 564 |
| Tru9I | TTAA | 1 cut(s) | 564 |
| TseFI | GTSAC | 1 cut(s) | 159 |
| Tsp45I | GTSAC | 1 cut(s) | 159 |
| TspDTI | ATGAA | 1 cut(s) | 375 |
| TspMI | CCCGGG | 1 cut(s) | 13 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 518 |
| XmaI | CCCGGG | 1 cut(s) | 13 |
| XspI | CTAG | 2 cut(s) | 260, 306 |