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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 509 |
| AclI | AACGTT | 1 cut(s) | 191 |
| AcoI | YGGCCR | 1 cut(s) | 249 |
| AcsI | RAATTY | 2 cut(s) | 212, 613 |
| AcuI | CTGAAG | 2 cut(s) | 194, 635 |
| AcyI | GRCGYC | 1 cut(s) | 506 |
| AdeI | CACNNNGTG | 1 cut(s) | 109 |
| AfaI | GTAC | 1 cut(s) | 667 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 112, 292, 624 |
| AgsI | TTSAA | 5 cut(s) | 63, 473, 514, 551, 610 |
| AjnI | CCWGG | 1 cut(s) | 105 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 401, 433 |
| AluBI | AGCT | 5 cut(s) | 299, 316, 350, 544, 686 |
| AluI | AGCT | 5 cut(s) | 299, 316, 350, 544, 686 |
| Alw21I | GWGCWC | 2 cut(s) | 51, 679 |
| Alw26I | GTCTC | 3 cut(s) | 373, 471, 517 |
| Ama87I | CYCGRG | 1 cut(s) | 425 |
| AoxI | GGCC | 3 cut(s) | 226, 249, 600 |
| ApeKI | GCWGC | 1 cut(s) | 199 |
| ApoI | RAATTY | 2 cut(s) | 212, 613 |
| AspA2I | CCTAGG | 1 cut(s) | 623 |
| AspLEI | GCGC | 1 cut(s) | 199 |
| AsuHPI | GGTGA | 2 cut(s) | 43, 320 |
| AvaI | CYCGRG | 1 cut(s) | 425 |
| AvrII | CCTAGG | 1 cut(s) | 623 |
| BalI | TGGCCA | 1 cut(s) | 251 |
| BanII | GRGCYC | 1 cut(s) | 172 |
| Bbv12I | GWGCWC | 2 cut(s) | 51, 679 |
| BbvI | GCAGC | 1 cut(s) | 211 |
| BccI | CCATC | 1 cut(s) | 158 |
| BciT130I | CCWGG | 1 cut(s) | 107 |
| BcoDI | GTCTC | 3 cut(s) | 373, 471, 517 |
| BfaI | CTAG | 3 cut(s) | 93, 317, 624 |
| BisI | GCNGC | 1 cut(s) | 200 |
| BlnI | CCTAGG | 1 cut(s) | 623 |
| BlsI | GCNGC | 1 cut(s) | 201 |
| Bme1390I | CCNGG | 1 cut(s) | 107 |
| BmeT110I | CYCGRG | 1 cut(s) | 425 |
| BmiI | GGNNCC | 1 cut(s) | 271 |
| BmrFI | CCNGG | 1 cut(s) | 107 |
| BpuEI | CTTGAG | 1 cut(s) | 659 |
| BsaHI | GRCGYC | 1 cut(s) | 506 |
| BsaJI | CCNNGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 112, 292, 624 |
| Bse1I | ACTGG | 1 cut(s) | 664 |
| BseBI | CCWGG | 1 cut(s) | 107 |
| BseDI | CCNNGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 112, 292, 624 |
| BseNI | ACTGG | 1 cut(s) | 664 |
| BseRI | GAGGAG | 1 cut(s) | 73 |
| BseXI | GCAGC | 1 cut(s) | 211 |
| BshFI | GGCC | 3 cut(s) | 228, 251, 602 |
| BsiHKAI | GWGCWC | 2 cut(s) | 51, 679 |
| BsiHKCI | CYCGRG | 1 cut(s) | 425 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 112, 292, 624 |
| BsmAI | GTCTC | 3 cut(s) | 373, 471, 517 |
| BsnI | GGCC | 3 cut(s) | 228, 251, 602 |
| BsoBI | CYCGRG | 1 cut(s) | 425 |
| Bsp1286I | GDGCHC | 3 cut(s) | 51, 172, 679 |
| Bsp19I | CCATGG | 1 cut(s) | 252 |
| BspANI | GGCC | 3 cut(s) | 228, 251, 602 |
| BspLI | GGNNCC | 1 cut(s) | 271 |
| BsrI | ACTGG | 1 cut(s) | 664 |
| BssECI | CCNNGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| BssNI | GRCGYC | 1 cut(s) | 506 |
| BssT1I | CCWWGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| Bst2UI | CCWGG | 1 cut(s) | 107 |
| BstACI | GRCGYC | 1 cut(s) | 506 |
| BstC8I | GCNNGC | 1 cut(s) | 69 |
| BstDSI | CCRYGG | 1 cut(s) | 252 |
| BstHHI | GCGC | 1 cut(s) | 199 |
| BstMAI | GTCTC | 3 cut(s) | 373, 471, 517 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 97, 573, 683, 692 |
| BstNI | CCWGG | 1 cut(s) | 107 |
| BstSCI | CCNGG | 1 cut(s) | 105 |
| BstV1I | GCAGC | 1 cut(s) | 211 |
| BsuRI | GGCC | 3 cut(s) | 228, 251, 602 |
| BtgI | CCRYGG | 1 cut(s) | 252 |
| BtsI | GCAGTG | 1 cut(s) | 569 |
| BtsIMutI | CAGTG | 2 cut(s) | 569, 657 |
| Cac8I | GCNNGC | 1 cut(s) | 69 |
| CfoI | GCGC | 1 cut(s) | 199 |
| CsiI | ACCWGGT | 1 cut(s) | 105 |
| Csp6I | GTAC | 1 cut(s) | 666 |
| CviAII | CATG | 1 cut(s) | 253 |
| CviQI | GTAC | 1 cut(s) | 666 |
| DraIII | CACNNNGTG | 1 cut(s) | 109 |
| EaeI | YGGCCR | 1 cut(s) | 249 |
| Eco130I | CCWWGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| Eco24I | GRGCYC | 1 cut(s) | 172 |
| Eco57I | CTGAAG | 2 cut(s) | 194, 635 |
| Eco88I | CYCGRG | 1 cut(s) | 425 |
| EcoRII | CCWGG | 1 cut(s) | 105 |
| EcoT14I | CCWWGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| EcoT38I | GRGCYC | 1 cut(s) | 172 |
| ErhI | CCWWGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| FaeI | CATG | 1 cut(s) | 256 |
| FaiI | YATR | 5 cut(s) | 32, 254, 358, 443, 632 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 27, 59, 401, 433, 551, 583 |
| FatI | CATG | 1 cut(s) | 252 |
| Fnu4HI | GCNGC | 1 cut(s) | 200 |
| FriOI | GRGCYC | 1 cut(s) | 172 |
| Fsp4HI | GCNGC | 1 cut(s) | 200 |
| FspBI | CTAG | 3 cut(s) | 93, 317, 624 |
| GlaI | GCGC | 1 cut(s) | 198 |
| GluI | GCNGC | 1 cut(s) | 200 |
| HaeIII | GGCC | 3 cut(s) | 228, 251, 602 |
| HhaI | GCGC | 1 cut(s) | 199 |
| Hin1I | GRCGYC | 1 cut(s) | 506 |
| Hin1II | CATG | 1 cut(s) | 256 |
| Hin6I | GCGC | 1 cut(s) | 197 |
| HinP1I | GCGC | 1 cut(s) | 197 |
| HincII | GTYRAC | 1 cut(s) | 327 |
| HindII | GTYRAC | 1 cut(s) | 327 |
| HindIII | AAGCTT | 1 cut(s) | 684 |
| HinfI | GANTC | 1 cut(s) | 464 |
| HphI | GGTGA | 2 cut(s) | 43, 320 |
| Hpy166II | GTNNAC | 1 cut(s) | 327 |
| Hpy188I | TCNGA | 2 cut(s) | 174, 619 |
| Hpy188III | TCNNGA | 3 cut(s) | 425, 470, 653 |
| Hpy8I | GTNNAC | 1 cut(s) | 327 |
| Hpy99I | CGWCG | 1 cut(s) | 91 |
| HpyAV | CCTTC | 4 cut(s) | 169, 467, 557, 659 |
| HpyCH4IV | ACGT | 2 cut(s) | 191, 506 |
| HpyCH4V | TGCA | 2 cut(s) | 139, 634 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 97, 573, 683, 692 |
| HpySE526I | ACGT | 2 cut(s) | 191, 506 |
| Hsp92I | GRCGYC | 1 cut(s) | 506 |
| Hsp92II | CATG | 1 cut(s) | 256 |
| HspAI | GCGC | 1 cut(s) | 197 |
| LmnI | GCTCC | 2 cut(s) | 269, 549 |
| LpnPI | CCDG | 7 cut(s) | 92, 119, 149, 210, 336, 638, 645 |
| Lsp1109I | GCAGC | 1 cut(s) | 211 |
| MabI | ACCWGGT | 1 cut(s) | 105 |
| MaeI | CTAG | 3 cut(s) | 93, 317, 624 |
| MaeII | ACGT | 2 cut(s) | 191, 506 |
| MaeIII | GTNAC | 3 cut(s) | 19, 261, 644 |
| MboII | GAAGA | 2 cut(s) | 47, 421 |
| MfeI | CAATTG | 2 cut(s) | 140, 500 |
| MhlI | GDGCHC | 3 cut(s) | 51, 172, 679 |
| MlsI | TGGCCA | 1 cut(s) | 251 |
| MluCI | AATT | 5 cut(s) | 140, 212, 500, 605, 613 |
| MluNI | TGGCCA | 1 cut(s) | 251 |
| MlyI | GAGTC | 1 cut(s) | 473 |
| MmeI | TCCRAC | 1 cut(s) | 471 |
| MnlI | CCTC | 5 cut(s) | 91, 94, 148, 297, 528 |
| Mox20I | TGGCCA | 1 cut(s) | 251 |
| MscI | TGGCCA | 1 cut(s) | 251 |
| MseI | TTAA | 2 cut(s) | 206, 681 |
| Msp20I | TGGCCA | 1 cut(s) | 251 |
| MspR9I | CCNGG | 1 cut(s) | 107 |
| MunI | CAATTG | 2 cut(s) | 140, 500 |
| MvaI | CCWGG | 1 cut(s) | 107 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 97, 573, 683, 692 |
| NcoI | CCATGG | 1 cut(s) | 252 |
| NlaIII | CATG | 1 cut(s) | 256 |
| NlaIV | GGNNCC | 1 cut(s) | 271 |
| NmuCI | GTSAC | 2 cut(s) | 19, 644 |
| PaeR7I | CTCGAG | 1 cut(s) | 425 |
| PkrI | GCNGC | 1 cut(s) | 201 |
| PleI | GAGTC | 1 cut(s) | 472 |
| PpsI | GAGTC | 1 cut(s) | 472 |
| Psp1406I | AACGTT | 1 cut(s) | 191 |
| Psp6I | CCWGG | 1 cut(s) | 105 |
| PspGI | CCWGG | 1 cut(s) | 105 |
| PspN4I | GGNNCC | 1 cut(s) | 271 |
| RsaI | GTAC | 1 cut(s) | 667 |
| RsaNI | GTAC | 1 cut(s) | 666 |
| SaqAI | TTAA | 2 cut(s) | 206, 681 |
| SatI | GCNGC | 1 cut(s) | 200 |
| SchI | GAGTC | 1 cut(s) | 473 |
| ScrFI | CCNGG | 1 cut(s) | 107 |
| SduI | GDGCHC | 3 cut(s) | 51, 172, 679 |
| SexAI | ACCWGGT | 1 cut(s) | 105 |
| Sfr274I | CTCGAG | 1 cut(s) | 425 |
| SlaI | CTCGAG | 1 cut(s) | 425 |
| SmlI | CTYRAG | 2 cut(s) | 425, 638 |
| SmoI | CTYRAG | 2 cut(s) | 425, 638 |
| Sse9I | AATT | 5 cut(s) | 140, 212, 500, 605, 613 |
| SspMI | CTAG | 3 cut(s) | 93, 317, 624 |
| StyD4I | CCNGG | 1 cut(s) | 105 |
| StyI | CCWWGG | 6 cut(s) | 252, 291, 417, 577, 623, 669 |
| TaiI | ACGT | 2 cut(s) | 194, 509 |
| TaqI | TCGA | 1 cut(s) | 426 |
| TasI | AATT | 5 cut(s) | 140, 212, 500, 605, 613 |
| Tru1I | TTAA | 2 cut(s) | 206, 681 |
| Tru9I | TTAA | 2 cut(s) | 206, 681 |
| TscAI | CASTG | 2 cut(s) | 569, 664 |
| TseFI | GTSAC | 2 cut(s) | 19, 644 |
| TseI | GCWGC | 1 cut(s) | 199 |
| Tsp45I | GTSAC | 2 cut(s) | 19, 644 |
| TspDTI | ATGAA | 3 cut(s) | 41, 345, 422 |
| TspRI | CASTG | 2 cut(s) | 569, 664 |
| XapI | RAATTY | 2 cut(s) | 212, 613 |
| XhoI | CTCGAG | 1 cut(s) | 425 |
| XmaJI | CCTAGG | 1 cut(s) | 623 |
| XspI | CTAG | 3 cut(s) | 93, 317, 624 |
| ZraI | GACGTC | 1 cut(s) | 507 |