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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 565 |
| AccIII | TCCGGA | 1 cut(s) | 6 |
| AciI | CCGC | 2 cut(s) | 615, 754 |
| AclWI | GGATC | 1 cut(s) | 435 |
| AcoI | YGGCCR | 3 cut(s) | 475, 559, 804 |
| AcsI | RAATTY | 1 cut(s) | 797 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 484 |
| AflIII | ACRYGT | 1 cut(s) | 682 |
| AgsI | TTSAA | 7 cut(s) | 245, 300, 484, 548, 572, 659, 764 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 235 |
| AhlI | ACTAGT | 1 cut(s) | 842 |
| AluBI | AGCT | 9 cut(s) | 22, 200, 248, 335, 349, 418, 493, 622, 722 |
| AluI | AGCT | 9 cut(s) | 22, 200, 248, 335, 349, 418, 493, 622, 722 |
| Alw21I | GWGCWC | 1 cut(s) | 24 |
| Alw26I | GTCTC | 2 cut(s) | 203, 311 |
| AlwI | GGATC | 1 cut(s) | 435 |
| Ama87I | CYCGRG | 2 cut(s) | 609, 772 |
| Aor13HI | TCCGGA | 1 cut(s) | 6 |
| AoxI | GGCC | 4 cut(s) | 90, 475, 559, 804 |
| ApeKI | GCWGC | 1 cut(s) | 200 |
| ApoI | RAATTY | 1 cut(s) | 797 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 260, 292 |
| AspLEI | GCGC | 2 cut(s) | 514, 636 |
| AspS9I | GGNCC | 1 cut(s) | 4 |
| AsuHPI | GGTGA | 4 cut(s) | 167, 236, 343, 743 |
| AvaI | CYCGRG | 2 cut(s) | 609, 772 |
| AvaII | GGWCC | 1 cut(s) | 4 |
| BalI | TGGCCA | 1 cut(s) | 806 |
| BanII | GRGCYC | 1 cut(s) | 24 |
| BbsI | GAAGAC | 1 cut(s) | 234 |
| Bbv12I | GWGCWC | 1 cut(s) | 24 |
| BbvI | GCAGC | 1 cut(s) | 187 |
| BccI | CCATC | 2 cut(s) | 68, 376 |
| BceAI | ACGGC | 3 cut(s) | 462, 490, 546 |
| BcoDI | GTCTC | 2 cut(s) | 203, 311 |
| BcuI | ACTAGT | 1 cut(s) | 842 |
| BfaI | CTAG | 3 cut(s) | 341, 741, 843 |
| BfmI | CTRYAG | 1 cut(s) | 690 |
| BisI | GCNGC | 2 cut(s) | 201, 615 |
| BlsI | GCNGC | 2 cut(s) | 202, 616 |
| Bme18I | GGWCC | 1 cut(s) | 4 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 235 |
| BmeT110I | CYCGRG | 2 cut(s) | 609, 772 |
| BmgT120I | GGNCC | 1 cut(s) | 4 |
| BpiI | GAAGAC | 1 cut(s) | 234 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 71, 103 |
| Bpu10I | CCTNAGC | 1 cut(s) | 331 |
| BsaBI | GATNNNNATC | 1 cut(s) | 764 |
| BsaWI | WCCGGW | 1 cut(s) | 6 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 576, 606 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 484 |
| Bse1I | ACTGG | 2 cut(s) | 204, 562 |
| Bse8I | GATNNNNATC | 1 cut(s) | 764 |
| BseAI | TCCGGA | 1 cut(s) | 6 |
| BseJI | GATNNNNATC | 1 cut(s) | 764 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 484 |
| BseMII | CTCAG | 1 cut(s) | 37 |
| BseNI | ACTGG | 2 cut(s) | 204, 562 |
| BseX3I | CGGCCG | 1 cut(s) | 475 |
| BseXI | GCAGC | 1 cut(s) | 187 |
| Bsh1285I | CGRYCG | 2 cut(s) | 478, 703 |
| BshFI | GGCC | 4 cut(s) | 92, 477, 561, 806 |
| BsiEI | CGRYCG | 2 cut(s) | 478, 703 |
| BsiHKAI | GWGCWC | 1 cut(s) | 24 |
| BsiHKCI | CYCGRG | 2 cut(s) | 609, 772 |
| BsiSI | CCGG | 1 cut(s) | 7 |
| BslFI | GGGAC | 3 cut(s) | 48, 566, 869 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 484 |
| BsmAI | GTCTC | 2 cut(s) | 203, 311 |
| BsmFI | GGGAC | 3 cut(s) | 48, 566, 869 |
| BsnI | GGCC | 4 cut(s) | 92, 477, 561, 806 |
| BsoBI | CYCGRG | 2 cut(s) | 609, 772 |
| Bsp1286I | GDGCHC | 1 cut(s) | 24 |
| Bsp13I | TCCGGA | 1 cut(s) | 6 |
| Bsp143I | GATC | 1 cut(s) | 427 |
| BspACI | CCGC | 2 cut(s) | 615, 754 |
| BspANI | GGCC | 4 cut(s) | 92, 477, 561, 806 |
| BspCNI | CTCAG | 1 cut(s) | 36 |
| BspEI | TCCGGA | 1 cut(s) | 6 |
| BspHI | TCATGA | 2 cut(s) | 286, 526 |
| BspMAI | CTGCAG | 1 cut(s) | 694 |
| BspPI | GGATC | 1 cut(s) | 435 |
| BspQI | GCTCTTC | 1 cut(s) | 483 |
| BsrI | ACTGG | 2 cut(s) | 204, 562 |
| BssMI | GATC | 1 cut(s) | 427 |
| Bst4CI | ACNGT | 2 cut(s) | 172, 704 |
| Bst6I | CTCTTC | 1 cut(s) | 483 |
| BstC8I | GCNNGC | 2 cut(s) | 20, 830 |
| BstDEI | CTNAG | 2 cut(s) | 23, 331 |
| BstHHI | GCGC | 2 cut(s) | 514, 636 |
| BstKTI | GATC | 1 cut(s) | 430 |
| BstMAI | GTCTC | 2 cut(s) | 203, 311 |
| BstMBI | GATC | 1 cut(s) | 427 |
| BstMCI | CGRYCG | 2 cut(s) | 478, 703 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 346, 410 |
| BstSFI | CTRYAG | 1 cut(s) | 690 |
| BstV1I | GCAGC | 1 cut(s) | 187 |
| BstV2I | GAAGAC | 1 cut(s) | 234 |
| BstX2I | RGATCY | 1 cut(s) | 427 |
| BstYI | RGATCY | 1 cut(s) | 427 |
| BstZI | CGGCCG | 1 cut(s) | 475 |
| BsuRI | GGCC | 4 cut(s) | 92, 477, 561, 806 |
| BtsIMutI | CAGTG | 2 cut(s) | 211, 555 |
| Cac8I | GCNNGC | 2 cut(s) | 20, 830 |
| CciI | TCATGA | 2 cut(s) | 286, 526 |
| CfoI | GCGC | 2 cut(s) | 514, 636 |
| Cfr13I | GGNCC | 1 cut(s) | 4 |
| CseI | GACGC | 1 cut(s) | 866 |
| CviAII | CATG | 5 cut(s) | 235, 287, 371, 527, 861 |
| DdeI | CTNAG | 2 cut(s) | 23, 331 |
| DpnI | GATC | 1 cut(s) | 429 |
| DpnII | GATC | 1 cut(s) | 427 |
| DriI | GACNNNNNGTC | 1 cut(s) | 235 |
| EaeI | YGGCCR | 3 cut(s) | 475, 559, 804 |
| EagI | CGGCCG | 1 cut(s) | 475 |
| Eam1104I | CTCTTC | 1 cut(s) | 483 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 235 |
| EarI | CTCTTC | 1 cut(s) | 483 |
| Ecl136II | GAGCTC | 1 cut(s) | 22 |
| EclXI | CGGCCG | 1 cut(s) | 475 |
| Eco24I | GRGCYC | 1 cut(s) | 24 |
| Eco47I | GGWCC | 1 cut(s) | 4 |
| Eco52I | CGGCCG | 1 cut(s) | 475 |
| Eco53kI | GAGCTC | 1 cut(s) | 22 |
| Eco88I | CYCGRG | 2 cut(s) | 609, 772 |
| EcoICRI | GAGCTC | 1 cut(s) | 22 |
| EcoT38I | GRGCYC | 1 cut(s) | 24 |
| FaeI | CATG | 5 cut(s) | 238, 290, 374, 530, 864 |
| FaqI | GGGAC | 3 cut(s) | 48, 566, 869 |
| FatI | CATG | 5 cut(s) | 234, 286, 370, 526, 860 |
| FblI | GTMKAC | 1 cut(s) | 565 |
| Fnu4HI | GCNGC | 2 cut(s) | 201, 615 |
| FriOI | GRGCYC | 1 cut(s) | 24 |
| Fsp4HI | GCNGC | 2 cut(s) | 201, 615 |
| FspBI | CTAG | 3 cut(s) | 341, 741, 843 |
| GlaI | GCGC | 2 cut(s) | 513, 635 |
| GluI | GCNGC | 2 cut(s) | 201, 615 |
| HaeIII | GGCC | 4 cut(s) | 92, 477, 561, 806 |
| HapII | CCGG | 1 cut(s) | 7 |
| HgaI | GACGC | 1 cut(s) | 866 |
| HhaI | GCGC | 2 cut(s) | 514, 636 |
| Hin1II | CATG | 5 cut(s) | 238, 290, 374, 530, 864 |
| Hin6I | GCGC | 2 cut(s) | 512, 634 |
| HinP1I | GCGC | 2 cut(s) | 512, 634 |
| HindIII | AAGCTT | 1 cut(s) | 416 |
| HinfI | GANTC | 2 cut(s) | 82, 871 |
| HpaII | CCGG | 1 cut(s) | 7 |
| HphI | GGTGA | 4 cut(s) | 167, 236, 343, 743 |
| Hpy166II | GTNNAC | 3 cut(s) | 566, 697, 746 |
| Hpy188I | TCNGA | 1 cut(s) | 26 |
| Hpy188III | TCNNGA | 7 cut(s) | 7, 287, 304, 527, 659, 698, 772 |
| Hpy8I | GTNNAC | 3 cut(s) | 566, 697, 746 |
| HpyAV | CCTTC | 3 cut(s) | 153, 215, 303 |
| HpyCH4III | ACNGT | 2 cut(s) | 172, 704 |
| HpyCH4IV | ACGT | 1 cut(s) | 682 |
| HpyCH4V | TGCA | 1 cut(s) | 692 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 346, 410 |
| HpyF3I | CTNAG | 2 cut(s) | 23, 331 |
| HpySE526I | ACGT | 1 cut(s) | 682 |
| Hsp92II | CATG | 5 cut(s) | 238, 290, 374, 530, 864 |
| HspAI | GCGC | 2 cut(s) | 512, 634 |
| Kpn2I | TCCGGA | 1 cut(s) | 6 |
| Kzo9I | GATC | 1 cut(s) | 427 |
| LguI | GCTCTTC | 1 cut(s) | 483 |
| LpnPI | CCDG | 4 cut(s) | 20, 217, 543, 725 |
| Lsp1109I | GCAGC | 1 cut(s) | 187 |
| MaeI | CTAG | 3 cut(s) | 341, 741, 843 |
| MaeII | ACGT | 1 cut(s) | 682 |
| MaeIII | GTNAC | 1 cut(s) | 685 |
| MalI | GATC | 1 cut(s) | 429 |
| MboI | GATC | 1 cut(s) | 427 |
| MboII | GAAGA | 8 cut(s) | 59, 239, 288, 500, 760, 827, 866, 880 |
| MfeI | CAATTG | 1 cut(s) | 240 |
| MflI | RGATCY | 1 cut(s) | 427 |
| MhlI | GDGCHC | 1 cut(s) | 24 |
| MlsI | TGGCCA | 1 cut(s) | 806 |
| MluCI | AATT | 7 cut(s) | 176, 240, 543, 577, 662, 797, 819 |
| MluNI | TGGCCA | 1 cut(s) | 806 |
| MmeI | TCCRAC | 2 cut(s) | 171, 754 |
| MnlI | CCTC | 7 cut(s) | 103, 194, 418, 479, 618, 768, 858 |
| Mox20I | TGGCCA | 1 cut(s) | 806 |
| MroI | TCCGGA | 1 cut(s) | 6 |
| MscI | TGGCCA | 1 cut(s) | 806 |
| MslI | CAYNNNNRTG | 1 cut(s) | 642 |
| Msp20I | TGGCCA | 1 cut(s) | 806 |
| MspI | CCGG | 1 cut(s) | 7 |
| MunI | CAATTG | 1 cut(s) | 240 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 346, 410 |
| NdeII | GATC | 1 cut(s) | 427 |
| NlaIII | CATG | 5 cut(s) | 238, 290, 374, 530, 864 |
| NmeAIII | GCCGAG | 2 cut(s) | 68, 694 |
| NmuCI | GTSAC | 1 cut(s) | 685 |
| PaeR7I | CTCGAG | 2 cut(s) | 609, 772 |
| PagI | TCATGA | 2 cut(s) | 286, 526 |
| PciSI | GCTCTTC | 1 cut(s) | 483 |
| PfeI | GAWTC | 2 cut(s) | 82, 871 |
| PkrI | GCNGC | 2 cut(s) | 202, 616 |
| Psp124BI | GAGCTC | 1 cut(s) | 24 |
| PspPI | GGNCC | 1 cut(s) | 4 |
| PspXI | VCTCGAGB | 1 cut(s) | 609 |
| PstI | CTGCAG | 1 cut(s) | 694 |
| PsuI | RGATCY | 1 cut(s) | 427 |
| RseI | CAYNNNNRTG | 1 cut(s) | 642 |
| SacI | GAGCTC | 1 cut(s) | 24 |
| SapI | GCTCTTC | 1 cut(s) | 483 |
| SatI | GCNGC | 2 cut(s) | 201, 615 |
| Sau3AI | GATC | 1 cut(s) | 427 |
| Sau96I | GGNCC | 1 cut(s) | 4 |
| SduI | GDGCHC | 1 cut(s) | 24 |
| SfcI | CTRYAG | 1 cut(s) | 690 |
| Sfr274I | CTCGAG | 2 cut(s) | 609, 772 |
| SinI | GGWCC | 1 cut(s) | 4 |
| SlaI | CTCGAG | 2 cut(s) | 609, 772 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 642 |
| SmlI | CTYRAG | 2 cut(s) | 609, 772 |
| SmoI | CTYRAG | 2 cut(s) | 609, 772 |
| SpeI | ACTAGT | 1 cut(s) | 842 |
| Sse9I | AATT | 7 cut(s) | 176, 240, 543, 577, 662, 797, 819 |
| SsiI | CCGC | 2 cut(s) | 615, 754 |
| SspMI | CTAG | 3 cut(s) | 341, 741, 843 |
| SstI | GAGCTC | 1 cut(s) | 24 |
| TaaI | ACNGT | 2 cut(s) | 172, 704 |
| TaiI | ACGT | 1 cut(s) | 685 |
| TaqI | TCGA | 4 cut(s) | 186, 303, 610, 773 |
| TaqII | GACCGA | 1 cut(s) | 84 |
| TasI | AATT | 7 cut(s) | 176, 240, 543, 577, 662, 797, 819 |
| TauI | GCSGC | 1 cut(s) | 617 |
| TfiI | GAWTC | 2 cut(s) | 82, 871 |
| TscAI | CASTG | 2 cut(s) | 211, 562 |
| TseFI | GTSAC | 1 cut(s) | 685 |
| TseI | GCWGC | 1 cut(s) | 200 |
| Tsp45I | GTSAC | 1 cut(s) | 685 |
| TspDTI | ATGAA | 4 cut(s) | 60, 210, 387, 543 |
| TspRI | CASTG | 2 cut(s) | 211, 562 |
| VpaK11BI | GGWCC | 1 cut(s) | 4 |
| XapI | RAATTY | 1 cut(s) | 797 |
| XhoI | CTCGAG | 2 cut(s) | 609, 772 |
| XmiI | GTMKAC | 1 cut(s) | 565 |
| XspI | CTAG | 3 cut(s) | 341, 741, 843 |