FvH4_4g05273

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000160
Physical Location & Seq
Reverse (-)
4551316 .. 4552866
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g05273.t1

Sequence Viewer

Length: 1551 bp
ATGGAGAAGTTGTGTCTTGCCCTATATTTTGCAGGGTGCAAGTTACGCCATTACATGCTTGCCTTTACCACGGTTGTGATCGCTCAATCCGACCTGGTCAAATACATGCTCTCTCGCCCTATCTTGCGGGGACGCATCGGCAAGTGGATCCTAGCCATGTCCGAATTCTCGTTGCAATATGTTCCGCAAAAGGCAGTAAAAGGACAGGCAATAGCAGACTTTCTCGCTCATCACCCACCCTCAGAGCTCACGGCGTTTCGCGAATTGGAATTCGCTGCTGTGGCACTCGCTCCATGGACCCTGTACTTTGCTGGTTCACGGACTGATACCGCAGCCGGAGCTGGGATAGCCATAGCCAATCCCACTAGAGATCGCTTCTCCTACTCGTTCCAGCTCGATTTCAAATGTACCAACAACCAGGCTGAGTACGAGGCGCTGATCATCAGCCTAGAGATTTTGTTGGATTTAGGAGTCCGCGAGGTTCAGATCTTTGGTGATTCTTTGTTGGTCGTTAACCAGCTGGTGGAAAAGTTTAAGTGCTTAAGCTCATCAATTGAGCCATACTTGCGCAAGGCATTTGAGGTGTTGGATCGATTTGACGACGTTTACATCGAGCACATACCACGCAAGTTCAACTTTGCCGCCAACGAGCTCGCCCAAGTAGCGTCCGGTCTCAGCTTGCGCGACGGCGTGCGCGAACGTTTGCTCAAAGTCGAACGCCGTACGCTTCCCTCTTTCATCGCTAGGGGGCAATTTCAGGCCAACACCCCCGAAGTTGCGGCCTTGGACCCTATCGACGAGGATTGGCGGCTACCATTTATCGCCTACCTCCAGAACCCCAACGACGCCGCTCACTCCAGGCAAATACGTTTCCTCGCCTTAAACTTTGTTTTACGCAATGGCGAACTCCGGCAGCGTGGGGAAGACGGAAACGATTTCCGTTGCGTATATGGGAACGAAGCTAAACGTATCATGCGCGAAGTCCATTATGGGGTTTGCGGTTCGCACCAGGCCGGGCCCAAAATGCGATGGTTGATCCGTCGCCATGGGTACTACTGGCCAACCATTCTGAAAAACTGTATCGCCTTCGCCAAAGGTTGCCAAGACTGCCAGGCTCACGGACCAGTGCAACATATTCCCAACATCCCGTTGCAGCCTATCATTAAACCTTGGCCGGGTCGCGGTTGGGCAATAGATTTCGTTGGCATTATTCATCCCCATTCTTCTGAGCAGCATAAGTTCATTATCGTCGCCACCGATTTCTTTACCAAGTGGGTCGAGGCCGAACCACTCAAGGTTGCCTCCGCCAACTCGGTTCGCAACTTTATTTTCCGCAATATCATCTCCCGCTTTGGTATCCCGGAGTGCATCGTTACAGACAGGGGAGCAGCTTTCATGGCTGATTCAGTTGTTAAGTACTTAAACGACTACGGAATCAAGCTTCTCCACTCTACGCCATATTATGCACAGTCCAACGGCCAAGCGGAGGCTAGTAATAAGGTCATCCTTGGCATCCTCCGCAAGATGTTGGAGTTGAACCCGCGTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

517

Amino Acids

58.54

Weight (kDa)

9.01

Isoelectric Point (pI)

37.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 106 - 221 1.3e-19 Reverse transcriptase-like
Integrase_H2C2 PF17921 320 - 373 9.6e-08 Integrase zinc binding domain
rve PF00665 392 - 488 2.5e-11 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 569
AccB7I CCANNNNNTGG 1 cut(s) 523
AccBSI CCGCTC 1 cut(s) 851
AccII CGCG 7 cut(s) 261, 477, 684, 696, 978, 1182, 1543
AclI AACGTT 1 cut(s) 700
AclWI GGATC 4 cut(s) 142, 155, 597, 1030
AcoI YGGCCR 3 cut(s) 1058, 1172, 1477
AcsI RAATTY 2 cut(s) 164, 269
AcyI GRCGYC 1 cut(s) 846
AfaI GTAC 6 cut(s) 305, 409, 428, 724, 1052, 1418
AfiI CCNNNNNNNGG 6 cut(s) 342, 523, 991, 1175, 1181, 1486
AflII CTTAAG 1 cut(s) 541
AgsI TTSAA 3 cut(s) 403, 634, 1537
AjnI CCWGG 5 cut(s) 93, 417, 857, 1008, 1110
AleI CACNNNNGTG 1 cut(s) 74
Alw21I GWGCWC 3 cut(s) 249, 618, 654
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 4 cut(s) 142, 155, 597, 1030
AoxI GGCC 8 cut(s) 759, 780, 1011, 1016, 1058, 1172, 1281, 1477
ApaI GGGCCC 1 cut(s) 1020
ApeKI GCWGC 6 cut(s) 275, 332, 913, 1153, 1231, 1388
ApoI RAATTY 2 cut(s) 164, 269
AspLEI GCGC 5 cut(s) 436, 570, 684, 696, 978
AspS9I GGNCC 5 cut(s) 297, 787, 1016, 1017, 1121
AsuC2I CCSGG 3 cut(s) 1015, 1176, 1361
AsuHPI GGTGA 2 cut(s) 224, 506
AvaII GGWCC 3 cut(s) 297, 787, 1121
BaeGI GKGCMC 1 cut(s) 1020
BalI TGGCCA 1 cut(s) 1060
BamHI GGATCC 1 cut(s) 147
BanII GRGCYC 3 cut(s) 249, 654, 1020
BbsI GAAGAC 1 cut(s) 930
Bbv12I GWGCWC 3 cut(s) 249, 618, 654
BbvI GCAGC 6 cut(s) 262, 344, 925, 1165, 1243, 1400
BccI CCATC 1 cut(s) 1023
BceAI ACGGC 4 cut(s) 267, 703, 705, 1492
BciT130I CCWGG 5 cut(s) 95, 419, 859, 1010, 1112
BciVI GTATCC 1 cut(s) 1367
BclI TGATCA 1 cut(s) 438
BcnI CCSGG 3 cut(s) 1015, 1176, 1361
BcoDI GTCTC 1 cut(s) 677
BfaI CTAG 6 cut(s) 152, 366, 449, 744, 1491, 1549
BfoI RGCGCY 1 cut(s) 437
BfrI CTTAAG 1 cut(s) 541
BfuI GTATCC 1 cut(s) 1367
BglII AGATCT 1 cut(s) 486
BmcAI AGTACT 1 cut(s) 1418
Bme1390I CCNGG 8 cut(s) 95, 419, 859, 1010, 1015, 1112, 1176, 1361
Bme18I GGWCC 3 cut(s) 297, 787, 1121
BmgT120I GGNCC 5 cut(s) 297, 787, 1016, 1017, 1121
BmiI GGNNCC 4 cut(s) 149, 299, 789, 1018
BmrFI CCNGG 8 cut(s) 95, 419, 859, 1010, 1015, 1112, 1176, 1361
BmsI GCATC 3 cut(s) 144, 1377, 1521
BpiI GAAGAC 1 cut(s) 930
BpmI CTGGAG 2 cut(s) 815, 841
BpuEI CTTGAG 1 cut(s) 1277
BpuMI CCSGG 3 cut(s) 1015, 1176, 1361
Bsa29I ATCGAT 1 cut(s) 592
BsaHI GRCGYC 1 cut(s) 846
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 6 cut(s) 69, 293, 783, 1045, 1169, 1507
BsaWI WCCGGW 1 cut(s) 668
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 6 cut(s) 342, 523, 991, 1175, 1181, 1486
Bse1I ACTGG 2 cut(s) 1061, 1124
Bse3DI GCAATG 1 cut(s) 904
BseBI CCWGG 5 cut(s) 95, 419, 859, 1010, 1112
BseCI ATCGAT 1 cut(s) 592
BseDI CCNNGG 6 cut(s) 69, 293, 783, 1045, 1169, 1507
BseGI GGATG 4 cut(s) 1143, 1213, 1503, 1512
BseLI CCNNNNNNNGG 6 cut(s) 342, 523, 991, 1175, 1181, 1486
BseMI GCAATG 1 cut(s) 904
BseMII CTCAG 4 cut(s) 255, 414, 688, 1218
BseNI ACTGG 2 cut(s) 1061, 1124
BseSI GKGCMC 1 cut(s) 1020
BseXI GCAGC 6 cut(s) 262, 344, 925, 1165, 1243, 1400
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 7 cut(s) 261, 477, 684, 696, 978, 1182, 1543
BshFI GGCC 8 cut(s) 761, 782, 1013, 1018, 1060, 1174, 1283, 1479
BshVI ATCGAT 1 cut(s) 592
BsiHKAI GWGCWC 3 cut(s) 249, 618, 654
BsiSI CCGG 6 cut(s) 336, 669, 910, 1014, 1175, 1361
BsiWI CGTACG 1 cut(s) 722
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 6 cut(s) 342, 523, 991, 1175, 1181, 1486
BsmAI GTCTC 1 cut(s) 677
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 8 cut(s) 761, 782, 1013, 1018, 1060, 1174, 1283, 1479
Bso31I GGTCTC 1 cut(s) 677
Bsp120I GGGCCC 1 cut(s) 1016
Bsp1286I GDGCHC 4 cut(s) 249, 618, 654, 1020
Bsp143I GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
Bsp19I CCATGG 2 cut(s) 293, 1045
Bsp68I TCGCGA 1 cut(s) 261
BspANI GGCC 8 cut(s) 761, 782, 1013, 1018, 1060, 1174, 1283, 1479
BspCNI CTCAG 4 cut(s) 254, 415, 687, 1219
BspDI ATCGAT 1 cut(s) 592
BspFNI CGCG 7 cut(s) 261, 477, 684, 696, 978, 1182, 1543
BspLI GGNNCC 4 cut(s) 149, 299, 789, 1018
BspPI GGATC 4 cut(s) 142, 155, 597, 1030
BspTI CTTAAG 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 904
BsrI ACTGG 2 cut(s) 1061, 1124
BssECI CCNNGG 6 cut(s) 69, 293, 783, 1045, 1169, 1507
BssMI GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
BssNI GRCGYC 1 cut(s) 846
BssT1I CCWWGG 5 cut(s) 293, 783, 1045, 1169, 1507
Bst2UI CCWGG 5 cut(s) 95, 419, 859, 1010, 1112
Bst4CI ACNGT 3 cut(s) 73, 1079, 1470
BstACI GRCGYC 1 cut(s) 846
BstAFI CTTAAG 1 cut(s) 541
BstC8I GCNNGC 4 cut(s) 60, 654, 680, 692
BstDEI CTNAG 4 cut(s) 241, 423, 674, 1227
BstDSI CCRYGG 3 cut(s) 69, 293, 1045
BstF5I GGATG 4 cut(s) 1143, 1213, 1503, 1512
BstFNI CGCG 7 cut(s) 261, 477, 684, 696, 978, 1182, 1543
BstH2I RGCGCY 1 cut(s) 437
BstHHI GCGC 5 cut(s) 436, 570, 684, 696, 978
BstKTI GATC 7 cut(s) 81, 150, 373, 441, 489, 592, 1038
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
BstNI CCWGG 5 cut(s) 95, 419, 859, 1010, 1112
BstNSI RCATGY 2 cut(s) 58, 109
BstSCI CCNGG 8 cut(s) 93, 417, 857, 1008, 1013, 1110, 1174, 1359
BstSLI GKGCMC 1 cut(s) 1020
BstUI CGCG 7 cut(s) 261, 477, 684, 696, 978, 1182, 1543
BstV1I GCAGC 6 cut(s) 262, 344, 925, 1165, 1243, 1400
BstV2I GAAGAC 1 cut(s) 930
BstX2I RGATCY 2 cut(s) 147, 486
BstYI RGATCY 2 cut(s) 147, 486
Bsu15I ATCGAT 1 cut(s) 592
BsuI GTATCC 1 cut(s) 1367
BsuRI GGCC 8 cut(s) 761, 782, 1013, 1018, 1060, 1174, 1283, 1479
BsuTUI ATCGAT 1 cut(s) 592
BtgI CCRYGG 3 cut(s) 69, 293, 1045
BtgZI GCGATG 2 cut(s) 724, 1042
BtsCI GGATG 4 cut(s) 1143, 1213, 1503, 1512
BtsIMutI CAGTG 1 cut(s) 1131
BtuMI TCGCGA 1 cut(s) 261
Cac8I GCNNGC 4 cut(s) 60, 654, 680, 692
CfoI GCGC 5 cut(s) 436, 570, 684, 696, 978
Cfr13I GGNCC 5 cut(s) 297, 787, 1016, 1017, 1121
ClaI ATCGAT 1 cut(s) 592
CseI GACGC 3 cut(s) 141, 654, 854
CsiI ACCWGGT 1 cut(s) 93
Csp6I GTAC 6 cut(s) 304, 408, 427, 723, 1051, 1417
CviAII CATG 7 cut(s) 55, 106, 157, 294, 973, 1046, 1396
CviQI GTAC 6 cut(s) 304, 408, 427, 723, 1051, 1417
DdeI CTNAG 4 cut(s) 241, 423, 674, 1227
DpnI GATC 7 cut(s) 80, 149, 372, 440, 488, 591, 1037
DpnII GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
EaeI YGGCCR 3 cut(s) 1058, 1172, 1477
EciI GGCGGA 1 cut(s) 1294
Ecl136II GAGCTC 2 cut(s) 247, 652
Eco130I CCWWGG 5 cut(s) 293, 783, 1045, 1169, 1507
Eco24I GRGCYC 3 cut(s) 249, 654, 1020
Eco31I GGTCTC 1 cut(s) 677
Eco47I GGWCC 3 cut(s) 297, 787, 1121
Eco53kI GAGCTC 2 cut(s) 247, 652
EcoICRI GAGCTC 2 cut(s) 247, 652
EcoRI GAATTC 2 cut(s) 164, 269
EcoRII CCWGG 5 cut(s) 93, 417, 857, 1008, 1110
EcoT14I CCWWGG 5 cut(s) 293, 783, 1045, 1169, 1507
EcoT38I GRGCYC 3 cut(s) 249, 654, 1020
ErhI CCWWGG 5 cut(s) 293, 783, 1045, 1169, 1507
FaeI CATG 7 cut(s) 58, 109, 160, 297, 976, 1049, 1399
FalI AAGNNNNNCTT 2 cut(s) 620, 652
FaqI GGGAC 1 cut(s) 144
FatI CATG 7 cut(s) 54, 105, 156, 293, 972, 1045, 1395
FauI CCCGC 3 cut(s) 120, 1355, 1548
FbaI TGATCA 1 cut(s) 438
FokI GGATG 4 cut(s) 1130, 1200, 1490, 1499
FriOI GRGCYC 3 cut(s) 249, 654, 1020
FspBI CTAG 6 cut(s) 152, 366, 449, 744, 1491, 1549
FspI TGCGCA 1 cut(s) 569
GlaI GCGC 5 cut(s) 435, 569, 683, 695, 977
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 2 cut(s) 815, 841
HaeII RGCGCY 1 cut(s) 437
HaeIII GGCC 8 cut(s) 761, 782, 1013, 1018, 1060, 1174, 1283, 1479
HapII CCGG 6 cut(s) 336, 669, 910, 1014, 1175, 1361
HgaI GACGC 3 cut(s) 141, 654, 854
HhaI GCGC 5 cut(s) 436, 570, 684, 696, 978
Hin1I GRCGYC 1 cut(s) 846
Hin1II CATG 7 cut(s) 58, 109, 160, 297, 976, 1049, 1399
Hin6I GCGC 5 cut(s) 434, 568, 682, 694, 976
HinP1I GCGC 5 cut(s) 434, 568, 682, 694, 976
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 1 cut(s) 1439
HinfI GANTC 4 cut(s) 471, 497, 1403, 1434
HpaI GTTAAC 1 cut(s) 514
HpaII CCGG 6 cut(s) 336, 669, 910, 1014, 1175, 1361
HphI GGTGA 2 cut(s) 224, 506
Hpy166II GTNNAC 3 cut(s) 317, 514, 607
Hpy188I TCNGA 6 cut(s) 91, 163, 244, 486, 1071, 1228
Hpy188III TCNNGA 2 cut(s) 260, 832
Hpy8I GTNNAC 3 cut(s) 317, 514, 607
Hpy99I CGWCG 6 cut(s) 605, 689, 800, 848, 1044, 1253
HpyAV CCTTC 1 cut(s) 1096
HpyCH4III ACNGT 3 cut(s) 73, 1079, 1470
HpyCH4IV ACGT 4 cut(s) 603, 700, 868, 967
HpyCH4V TGCA 7 cut(s) 32, 39, 175, 1129, 1153, 1368, 1466
HpyF3I CTNAG 4 cut(s) 241, 423, 674, 1227
HpySE526I ACGT 4 cut(s) 603, 700, 868, 967
Hsp92I GRCGYC 1 cut(s) 846
Hsp92II CATG 7 cut(s) 58, 109, 160, 297, 976, 1049, 1399
HspAI GCGC 5 cut(s) 434, 568, 682, 694, 976
Ksp22I TGATCA 1 cut(s) 438
KspAI GTTAAC 1 cut(s) 514
Kzo9I GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
LmnI GCTCC 3 cut(s) 295, 338, 1385
Lsp1109I GCAGC 6 cut(s) 262, 344, 925, 1165, 1243, 1400
LweI GCATC 3 cut(s) 144, 1377, 1521
MabI ACCWGGT 1 cut(s) 93
MaeI CTAG 6 cut(s) 152, 366, 449, 744, 1491, 1549
MaeII ACGT 4 cut(s) 603, 700, 868, 967
MaeIII GTNAC 2 cut(s) 42, 1372
MalI GATC 7 cut(s) 80, 149, 372, 440, 488, 591, 1037
MbiI CCGCTC 1 cut(s) 851
MboI GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
MboII GAAGA 2 cut(s) 935, 1215
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 2 cut(s) 147, 486
MhlI GDGCHC 4 cut(s) 249, 618, 654, 1020
MlsI TGGCCA 1 cut(s) 1060
MluCI AATT 5 cut(s) 164, 263, 269, 552, 752
MluNI TGGCCA 1 cut(s) 1060
MlyI GAGTC 1 cut(s) 480
MmeI TCCRAC 5 cut(s) 114, 441, 567, 1497, 1509
Mox20I TGGCCA 1 cut(s) 1060
MscI TGGCCA 1 cut(s) 1060
MseI TTAA 7 cut(s) 513, 534, 542, 881, 1164, 1413, 1421
MslI CAYNNNNRTG 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 1060
MspA1I CMGCKG 1 cut(s) 520
MspCI CTTAAG 1 cut(s) 541
MspI CCGG 6 cut(s) 336, 669, 910, 1014, 1175, 1361
MspR9I CCNGG 8 cut(s) 95, 419, 859, 1010, 1015, 1112, 1176, 1361
MunI CAATTG 1 cut(s) 552
MvaI CCWGG 5 cut(s) 95, 419, 859, 1010, 1112
MvnI CGCG 7 cut(s) 261, 477, 684, 696, 978, 1182, 1543
NciI CCSGG 3 cut(s) 1015, 1176, 1361
NcoI CCATGG 2 cut(s) 293, 1045
NdeII GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
NlaIII CATG 7 cut(s) 58, 109, 160, 297, 976, 1049, 1399
NlaIV GGNNCC 4 cut(s) 149, 299, 789, 1018
NruI TCGCGA 1 cut(s) 261
NsbI TGCGCA 1 cut(s) 569
NspI RCATGY 2 cut(s) 58, 109
OliI CACNNNNGTG 1 cut(s) 74
PcsI WCGNNNNNNNCGW 4 cut(s) 87, 609, 693, 1254
PfeI GAWTC 3 cut(s) 497, 1403, 1434
Pfl23II CGTACG 1 cut(s) 722
PflFI GACNNNGTC 1 cut(s) 95
PflMI CCANNNNNTGG 1 cut(s) 523
PfoI TCCNGGA 1 cut(s) 1359
PleI GAGTC 1 cut(s) 479
PpsI GAGTC 1 cut(s) 479
Psp124BI GAGCTC 2 cut(s) 249, 654
Psp1406I AACGTT 1 cut(s) 700
Psp6I CCWGG 5 cut(s) 93, 417, 857, 1008, 1110
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 5 cut(s) 93, 417, 857, 1008, 1110
PspLI CGTACG 1 cut(s) 722
PspN4I GGNNCC 4 cut(s) 149, 299, 789, 1018
PspOMI GGGCCC 1 cut(s) 1016
PspPI GGNCC 5 cut(s) 297, 787, 1016, 1017, 1121
PsuI RGATCY 2 cut(s) 147, 486
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 520
RruI TCGCGA 1 cut(s) 261
RsaI GTAC 6 cut(s) 305, 409, 428, 724, 1052, 1418
RsaNI GTAC 6 cut(s) 304, 408, 427, 723, 1051, 1417
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 2 cut(s) 249, 654
SaqAI TTAA 7 cut(s) 513, 534, 542, 881, 1164, 1413, 1421
Sau3AI GATC 7 cut(s) 78, 147, 370, 438, 486, 589, 1035
Sau96I GGNCC 5 cut(s) 297, 787, 1016, 1017, 1121
ScaI AGTACT 1 cut(s) 1418
SchI GAGTC 1 cut(s) 480
ScrFI CCNGG 8 cut(s) 95, 419, 859, 1010, 1015, 1112, 1176, 1361
SduI GDGCHC 4 cut(s) 249, 618, 654, 1020
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 3 cut(s) 144, 1377, 1521
SinI GGWCC 3 cut(s) 297, 787, 1121
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 2 cut(s) 541, 1292
SmoI CTYRAG 2 cut(s) 541, 1292
Sse9I AATT 5 cut(s) 164, 263, 269, 552, 752
SspMI CTAG 6 cut(s) 152, 366, 449, 744, 1491, 1549
SstI GAGCTC 2 cut(s) 249, 654
StyD4I CCNGG 8 cut(s) 93, 417, 857, 1008, 1013, 1110, 1174, 1359
StyI CCWWGG 5 cut(s) 293, 783, 1045, 1169, 1507
TaaI ACNGT 3 cut(s) 73, 1079, 1470
TaiI ACGT 4 cut(s) 606, 703, 871, 970
TaqI TCGA 6 cut(s) 396, 592, 612, 714, 795, 1278
TasI AATT 5 cut(s) 164, 263, 269, 552, 752
TatI WGTACW 2 cut(s) 303, 1416
TauI GCSGC 4 cut(s) 644, 782, 811, 851
TfiI GAWTC 3 cut(s) 497, 1403, 1434
Tru1I TTAA 7 cut(s) 513, 534, 542, 881, 1164, 1413, 1421
Tru9I TTAA 7 cut(s) 513, 534, 542, 881, 1164, 1413, 1421
TscAI CASTG 1 cut(s) 1131
TseI GCWGC 6 cut(s) 275, 332, 913, 1153, 1231, 1388
TspDTI ATGAA 4 cut(s) 727, 1202, 1231, 1384
TspGWI ACGGA 6 cut(s) 334, 929, 942, 1028, 1134, 1446
TspRI CASTG 1 cut(s) 1131
Tth111I GACNNNGTC 1 cut(s) 95
Van91I CCANNNNNTGG 1 cut(s) 523
Vha464I CTTAAG 1 cut(s) 541
VpaK11BI GGWCC 3 cut(s) 297, 787, 1121
XapI RAATTY 2 cut(s) 164, 269
XceI RCATGY 2 cut(s) 58, 109
XspI CTAG 6 cut(s) 152, 366, 449, 744, 1491, 1549
ZrmI AGTACT 1 cut(s) 1418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.