Rw7G025850

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000160
Physical Location & Seq
Reverse (-)
32642037 .. 32643409
1373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025850.1

Sequence Viewer

Length: 1101 bp
ATGCAGCTACACAAGCTCAAGATGAATCCCGCCAAGTGCGTTTTTGGAGTTCAGGCAGGAGATTTCCTGGGATTCATTGTCCATCAAAGAGGCATTGAGGTCCCTGAGGATAAAGCAAACGCGTTGCAAGGACAGAATGAGTTTGTGTGGGAACCTAAACATCAAGAGGCTTTCGACAAAATCAAGGCCTATCTGGCGAGCCCGCCAGTGCTTGTTCCTCCTAGAGCGGGATTTCCATTAAAGTTGTATGTTTCAGCAGCTGAGGCTTCCATTGGCAGCCTCCTCGCTCAGGATGATGCAAATGGTGTTGAACATGCCATCTTTTACCTCAGTAGGACACTCACAGATTGCGAAACAAGGTACACTCCAATGGAAAAGCTGTGTCTTACATTATACTTCTCAGCATGCAAGTTGCGGCATTACATGTTGTCCTTTACTACTTGCATCATTGCTCAAACCGATCTAAAAGCAGTAAAGGGGCAAGCCATAGCAGACTTTCTGGCACATCACCCTATGCTAGATGTCCCCGCGGTTAGAGATTTAGAGGTCGCTGCCACAACTTTAGATCGCCCGGATTTAGCGTGCTTGCCAGAGTACGCCGCGCTTTATCAAGCCACAGTCTCACTCCAGCCTTGGGTGTTATATTTTGACGGGTCAAGAACAGATACGCTAGCAGGAGCAGGGGTTGTCTTGGAAAACCCGGCCGGCGATCGATTTTCCTACTCTTTCCAGTTGGAGTTCCAGTGTACCAATAACCAAGCAGAGTATGAGGCCCTCATTATAGGCCTAGAAGTACTACTGGAAATGGGAATCAGGGATGTACAAATACTTGGCGACTCTCTCTTGGTTATCAATCAGGCATTGTACCTGTTGGACCAGTTTGATAACATACATCTAGAGTACATTCCTCGCGAGCGAAATTTTGCAGCGAACGAGTTGGCCCAACTGGCAACAGGGGTAACATTGAGATATGGCGTGCGGGAAAGAATCCTTAAAGTCGAGCGTCGCACGCTTCCTTCATGGATGCGAGAAGAGATCCTCCAGATGATACCTCAGTCGCGACCCTGGAACCCATTGACGTGGATTGGCGCATCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

366

Amino Acids

41.14

Weight (kDa)

5.26

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 50 - 144 1.4e-21 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 79 - 159 3.4e-13 RNase H-like domain found in reverse transcriptase
RVT_3 PF13456 216 - 286 1.7e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 227
AccII CGCG 5 cut(s) 122, 530, 602, 912, 1060
AciI CCGC 8 cut(s) 30, 203, 227, 415, 528, 530, 600, 979
AclWI GGATC 1 cut(s) 1030
AcoI YGGCCR 1 cut(s) 702
AcsI RAATTY 1 cut(s) 919
AfaI GTAC 7 cut(s) 362, 596, 748, 795, 822, 866, 902
AfiI CCNNNNNNNGG 3 cut(s) 227, 289, 634
AflIII ACRYGT 2 cut(s) 120, 423
AgsI TTSAA 1 cut(s) 311
AjiI CACGTC 1 cut(s) 1080
AjnI CCWGG 2 cut(s) 66, 1064
AluBI AGCT 4 cut(s) 7, 16, 260, 379
AluI AGCT 4 cut(s) 7, 16, 260, 379
Alw26I GTCTC 1 cut(s) 625
AlwI GGATC 1 cut(s) 1030
AlwNI CAGNNNCTG 1 cut(s) 260
AoxI GGCC 5 cut(s) 186, 702, 771, 784, 939
ApeKI GCWGC 5 cut(s) 4, 257, 276, 551, 926
ApoI RAATTY 1 cut(s) 919
AspLEI GCGC 2 cut(s) 604, 1091
AspS9I GGNCC 4 cut(s) 100, 772, 874, 940
AsuC2I CCSGG 2 cut(s) 572, 701
AsuHPI GGTGA 1 cut(s) 500
AsuNHI GCTAGC 1 cut(s) 670
AvaII GGWCC 2 cut(s) 100, 874
AxyI CCTNAGG 1 cut(s) 105
BaeI ACNNNNGTAYC 2 cut(s) 1040, 1073
BanII GRGCYC 1 cut(s) 203
BbvCI CCTCAGC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 16, 269, 288, 538, 938
BccI CCATC 2 cut(s) 90, 326
BcgI CGANNNNNNTGC 2 cut(s) 265, 299
BciT130I CCWGG 2 cut(s) 68, 1066
BcnI CCSGG 2 cut(s) 572, 701
BcoDI GTCTC 1 cut(s) 625
BfaI CTAG 5 cut(s) 222, 518, 671, 788, 896
BglI GCCNNNNNGGC 2 cut(s) 194, 947
BisI GCNGC 7 cut(s) 5, 258, 277, 416, 552, 600, 927
BlsI GCNGC 7 cut(s) 6, 259, 278, 417, 553, 601, 928
BmcAI AGTACT 1 cut(s) 795
Bme1390I CCNGG 4 cut(s) 68, 572, 701, 1066
Bme18I GGWCC 2 cut(s) 100, 874
BmgBI CACGTC 1 cut(s) 1080
BmgT120I GGNCC 4 cut(s) 100, 772, 874, 940
BmiI GGNNCC 3 cut(s) 102, 153, 1070
BmrFI CCNGG 4 cut(s) 68, 572, 701, 1066
BmsI GCATC 3 cut(s) 286, 453, 1014
BmtI GCTAGC 1 cut(s) 674
BpmI CTGGAG 2 cut(s) 611, 1025
Bpu10I CCTNAGC 2 cut(s) 261, 288
BpuMI CCSGG 2 cut(s) 572, 701
Bsa29I ATCGAT 1 cut(s) 712
BsaJI CCNNGG 4 cut(s) 67, 528, 632, 1064
BsaXI ACNNNNNCTCC 4 cut(s) 669, 699, 728, 758
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 289, 634
Bse118I RCCGGY 1 cut(s) 704
Bse1I ACTGG 6 cut(s) 206, 730, 742, 804, 877, 951
Bse21I CCTNAGG 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 447
BseBI CCWGG 2 cut(s) 68, 1066
BseCI ATCGAT 1 cut(s) 712
BseDI CCNNGG 4 cut(s) 67, 528, 632, 1064
BseGI GGATG 4 cut(s) 298, 823, 1029, 1091
BseLI CCNNNNNNNGG 3 cut(s) 227, 289, 634
BseMI GCAATG 1 cut(s) 447
BseMII CTCAG 6 cut(s) 96, 252, 302, 343, 414, 1067
BseNI ACTGG 6 cut(s) 206, 730, 742, 804, 877, 951
BseRI GAGGAG 1 cut(s) 272
BseX3I CGGCCG 1 cut(s) 702
BseXI GCAGC 5 cut(s) 16, 269, 288, 538, 938
Bsh1236I CGCG 5 cut(s) 122, 530, 602, 912, 1060
Bsh1285I CGRYCG 2 cut(s) 705, 712
BshFI GGCC 5 cut(s) 188, 704, 773, 786, 941
BshVI ATCGAT 1 cut(s) 712
BsiEI CGRYCG 2 cut(s) 705, 712
BsiSI CCGG 3 cut(s) 572, 701, 705
BslFI GGGAC 2 cut(s) 86, 509
BslI CCNNNNNNNGG 3 cut(s) 227, 289, 634
BsmAI GTCTC 1 cut(s) 625
BsmFI GGGAC 2 cut(s) 86, 509
BsnI GGCC 5 cut(s) 188, 704, 773, 786, 941
Bsp1286I GDGCHC 1 cut(s) 203
Bsp1407I TGTACA 1 cut(s) 820
Bsp143I GATC 4 cut(s) 460, 565, 709, 1035
Bsp68I TCGCGA 2 cut(s) 912, 1060
BspACI CCGC 8 cut(s) 30, 203, 227, 415, 528, 530, 600, 979
BspANI GGCC 5 cut(s) 188, 704, 773, 786, 941
BspCNI CTCAG 6 cut(s) 97, 253, 301, 342, 413, 1066
BspDI ATCGAT 1 cut(s) 712
BspFNI CGCG 5 cut(s) 122, 530, 602, 912, 1060
BspLI GGNNCC 3 cut(s) 102, 153, 1070
BspOI GCTAGC 1 cut(s) 674
BspPI GGATC 1 cut(s) 1030
BsrBI CCGCTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 447
BsrFI RCCGGY 1 cut(s) 704
BsrGI TGTACA 1 cut(s) 820
BsrI ACTGG 6 cut(s) 206, 730, 742, 804, 877, 951
BssAI RCCGGY 1 cut(s) 704
BssECI CCNNGG 4 cut(s) 67, 528, 632, 1064
BssMI GATC 4 cut(s) 460, 565, 709, 1035
BssT1I CCWWGG 1 cut(s) 632
Bst2UI CCWGG 2 cut(s) 68, 1066
Bst4CI ACNGT 1 cut(s) 619
Bst6I CTCTTC 1 cut(s) 1026
BstAUI TGTACA 1 cut(s) 820
BstDEI CTNAG 6 cut(s) 105, 261, 288, 329, 400, 1053
BstDSI CCRYGG 1 cut(s) 528
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 4 cut(s) 298, 823, 1029, 1091
BstFNI CGCG 5 cut(s) 122, 530, 602, 912, 1060
BstHHI GCGC 2 cut(s) 604, 1091
BstKTI GATC 4 cut(s) 463, 568, 712, 1038
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 4 cut(s) 460, 565, 709, 1035
BstMCI CGRYCG 2 cut(s) 705, 712
BstMWI GCNNNNNNNGC 5 cut(s) 13, 194, 263, 947, 1009
BstNI CCWGG 2 cut(s) 68, 1066
BstNSI RCATGY 3 cut(s) 317, 408, 427
BstSCI CCNGG 4 cut(s) 66, 570, 699, 1064
BstUI CGCG 5 cut(s) 122, 530, 602, 912, 1060
BstV1I GCAGC 5 cut(s) 16, 269, 288, 538, 938
BstX2I RGATCY 1 cut(s) 1035
BstXI CCANNNNNNTGG 1 cut(s) 1080
BstYI RGATCY 1 cut(s) 1035
BstZI CGGCCG 1 cut(s) 702
Bsu15I ATCGAT 1 cut(s) 712
Bsu36I CCTNAGG 1 cut(s) 105
BsuRI GGCC 5 cut(s) 188, 704, 773, 786, 941
BsuTUI ATCGAT 1 cut(s) 712
BtgI CCRYGG 1 cut(s) 528
BtrI CACGTC 1 cut(s) 1080
BtsCI GGATG 4 cut(s) 298, 823, 1029, 1091
BtsIMutI CAGTG 2 cut(s) 213, 749
BtuMI TCGCGA 2 cut(s) 912, 1060
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 2 cut(s) 604, 1091
Cfr10I RCCGGY 1 cut(s) 704
Cfr13I GGNCC 4 cut(s) 100, 772, 874, 940
Cfr42I CCGCGG 1 cut(s) 531
ClaI ATCGAT 1 cut(s) 712
CseI GACGC 1 cut(s) 992
Csp6I GTAC 7 cut(s) 361, 595, 747, 794, 821, 865, 901
CviAII CATG 4 cut(s) 314, 405, 424, 1020
CviQI GTAC 7 cut(s) 361, 595, 747, 794, 821, 865, 901
DdeI CTNAG 6 cut(s) 105, 261, 288, 329, 400, 1053
DpnI GATC 4 cut(s) 462, 567, 711, 1037
DpnII GATC 4 cut(s) 460, 565, 709, 1035
EaeI YGGCCR 1 cut(s) 702
EagI CGGCCG 1 cut(s) 702
Eam1104I CTCTTC 1 cut(s) 1026
EarI CTCTTC 1 cut(s) 1026
EclXI CGGCCG 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 632
Eco147I AGGCCT 2 cut(s) 188, 786
Eco24I GRGCYC 1 cut(s) 203
Eco47I GGWCC 2 cut(s) 100, 874
Eco52I CGGCCG 1 cut(s) 702
Eco81I CCTNAGG 1 cut(s) 105
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoO109I RGGNCCY 2 cut(s) 100, 772
EcoRII CCWGG 2 cut(s) 66, 1064
EcoT14I CCWWGG 1 cut(s) 632
EcoT38I GRGCYC 1 cut(s) 203
ErhI CCWWGG 1 cut(s) 632
FaeI CATG 4 cut(s) 317, 408, 427, 1023
FaqI GGGAC 2 cut(s) 86, 509
FatI CATG 4 cut(s) 313, 404, 423, 1019
FauI CCCGC 5 cut(s) 37, 210, 220, 535, 972
Fnu4HI GCNGC 7 cut(s) 5, 258, 277, 416, 552, 600, 927
FokI GGATG 4 cut(s) 305, 830, 1036, 1078
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 7 cut(s) 5, 258, 277, 416, 552, 600, 927
FspBI CTAG 5 cut(s) 222, 518, 671, 788, 896
GlaI GCGC 2 cut(s) 603, 1090
GluI GCNGC 7 cut(s) 5, 258, 277, 416, 552, 600, 927
GsuI CTGGAG 2 cut(s) 611, 1025
HaeIII GGCC 5 cut(s) 188, 704, 773, 786, 941
HapII CCGG 3 cut(s) 572, 701, 705
HgaI GACGC 1 cut(s) 992
HhaI GCGC 2 cut(s) 604, 1091
Hin1II CATG 4 cut(s) 317, 408, 427, 1023
Hin6I GCGC 2 cut(s) 602, 1089
HinP1I GCGC 2 cut(s) 602, 1089
HinfI GANTC 5 cut(s) 25, 72, 810, 836, 987
HpaII CCGG 3 cut(s) 572, 701, 705
HphI GGTGA 1 cut(s) 500
Hpy166II GTNNAC 2 cut(s) 363, 747
Hpy188III TCNNGA 8 cut(s) 19, 164, 290, 657, 896, 911, 1042, 1059
Hpy8I GTNNAC 2 cut(s) 363, 747
Hpy99I CGWCG 1 cut(s) 1008
HpyAV CCTTC 1 cut(s) 1026
HpyCH4III ACNGT 1 cut(s) 619
HpyCH4IV ACGT 1 cut(s) 1079
HpyCH4V TGCA 6 cut(s) 4, 127, 299, 408, 444, 926
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 194, 263, 947, 1009
HpyF3I CTNAG 6 cut(s) 105, 261, 288, 329, 400, 1053
HpySE526I ACGT 1 cut(s) 1079
Hsp92II CATG 4 cut(s) 317, 408, 427, 1023
HspAI GCGC 2 cut(s) 602, 1089
KroI GCCGGC 1 cut(s) 704
KroNI GCCGGC 1 cut(s) 706
KspI CCGCGG 1 cut(s) 531
Kzo9I GATC 4 cut(s) 460, 565, 709, 1035
LmnI GCTCC 1 cut(s) 677
Lsp1109I GCAGC 5 cut(s) 16, 269, 288, 538, 938
LweI GCATC 3 cut(s) 286, 453, 1014
MaeI CTAG 5 cut(s) 222, 518, 671, 788, 896
MaeII ACGT 1 cut(s) 1079
MaeIII GTNAC 1 cut(s) 958
MalI GATC 4 cut(s) 462, 567, 711, 1037
MbiI CCGCTC 1 cut(s) 227
MboI GATC 4 cut(s) 460, 565, 709, 1035
MboII GAAGA 1 cut(s) 1043
MflI RGATCY 1 cut(s) 1035
MhlI GDGCHC 1 cut(s) 203
MluCI AATT 1 cut(s) 919
MluI ACGCGT 1 cut(s) 120
MlyI GAGTC 1 cut(s) 830
MmeI TCCRAC 2 cut(s) 714, 852
MroNI GCCGGC 1 cut(s) 704
MseI TTAA 2 cut(s) 239, 993
MslI CAYNNNNRTG 2 cut(s) 368, 1078
MspA1I CMGCKG 2 cut(s) 260, 530
MspI CCGG 3 cut(s) 572, 701, 705
MspR9I CCNGG 4 cut(s) 68, 572, 701, 1066
MvaI CCWGG 2 cut(s) 68, 1066
MvnI CGCG 5 cut(s) 122, 530, 602, 912, 1060
MwoI GCNNNNNNNGC 5 cut(s) 13, 194, 263, 947, 1009
NaeI GCCGGC 1 cut(s) 706
NciI CCSGG 2 cut(s) 572, 701
NdeII GATC 4 cut(s) 460, 565, 709, 1035
NgoMIV GCCGGC 1 cut(s) 704
NheI GCTAGC 1 cut(s) 670
NlaIII CATG 4 cut(s) 317, 408, 427, 1023
NlaIV GGNNCC 3 cut(s) 102, 153, 1070
NruI TCGCGA 2 cut(s) 912, 1060
NspI RCATGY 3 cut(s) 317, 408, 427
PaeI GCATGC 1 cut(s) 408
PceI AGGCCT 2 cut(s) 188, 786
PciI ACATGT 1 cut(s) 423
PdiI GCCGGC 1 cut(s) 706
PfeI GAWTC 4 cut(s) 25, 72, 810, 987
PkrI GCNGC 7 cut(s) 6, 259, 278, 417, 553, 601, 928
Ple19I CGATCG 1 cut(s) 712
PleI GAGTC 1 cut(s) 830
PpsI GAGTC 1 cut(s) 830
PpuMI RGGWCCY 1 cut(s) 100
PscI ACATGT 1 cut(s) 423
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 66, 1064
PspGI CCWGG 2 cut(s) 66, 1064
PspN4I GGNNCC 3 cut(s) 102, 153, 1070
PspPI GGNCC 4 cut(s) 100, 772, 874, 940
PspPPI RGGWCCY 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 260
PsuI RGATCY 1 cut(s) 1035
PvuI CGATCG 1 cut(s) 712
PvuII CAGCTG 1 cut(s) 260
RruI TCGCGA 2 cut(s) 912, 1060
RsaI GTAC 7 cut(s) 362, 596, 748, 795, 822, 866, 902
RsaNI GTAC 7 cut(s) 361, 595, 747, 794, 821, 865, 901
RseI CAYNNNNRTG 2 cut(s) 368, 1078
SacII CCGCGG 1 cut(s) 531
SaqAI TTAA 2 cut(s) 239, 993
SatI GCNGC 7 cut(s) 5, 258, 277, 416, 552, 600, 927
Sau3AI GATC 4 cut(s) 460, 565, 709, 1035
Sau96I GGNCC 4 cut(s) 100, 772, 874, 940
ScaI AGTACT 1 cut(s) 795
SchI GAGTC 1 cut(s) 830
ScrFI CCNGG 4 cut(s) 68, 572, 701, 1066
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 3 cut(s) 286, 453, 1014
Sfr303I CCGCGG 1 cut(s) 531
SgrBI CCGCGG 1 cut(s) 531
SinI GGWCC 2 cut(s) 100, 874
SmiMI CAYNNNNRTG 2 cut(s) 368, 1078
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
SphI GCATGC 1 cut(s) 408
Sse9I AATT 1 cut(s) 919
SseBI AGGCCT 2 cut(s) 188, 786
SsiI CCGC 8 cut(s) 30, 203, 227, 415, 528, 530, 600, 979
SspMI CTAG 5 cut(s) 222, 518, 671, 788, 896
StuI AGGCCT 2 cut(s) 188, 786
StyD4I CCNGG 4 cut(s) 66, 570, 699, 1064
StyI CCWWGG 1 cut(s) 632
TaaI ACNGT 1 cut(s) 619
TaiI ACGT 1 cut(s) 1082
TaqI TCGA 3 cut(s) 174, 712, 999
TasI AATT 1 cut(s) 919
TatI WGTACW 3 cut(s) 793, 820, 900
TauI GCSGC 2 cut(s) 418, 602
TfiI GAWTC 4 cut(s) 25, 72, 810, 987
Tru1I TTAA 2 cut(s) 239, 993
Tru9I TTAA 2 cut(s) 239, 993
TscAI CASTG 2 cut(s) 213, 749
TseI GCWGC 5 cut(s) 4, 257, 276, 551, 926
TspDTI ATGAA 3 cut(s) 38, 64, 1008
TspRI CASTG 2 cut(s) 213, 749
VpaK11BI GGWCC 2 cut(s) 100, 874
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 919
XbaI TCTAGA 1 cut(s) 895
XceI RCATGY 3 cut(s) 317, 408, 427
XspI CTAG 5 cut(s) 222, 518, 671, 788, 896
ZrmI AGTACT 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.