pycom12g08310

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000160
Physical Location & Seq
Reverse (-)
9488055 .. 9488802
748 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g08310.1

Sequence Viewer

Length: 687 bp
ATGCTCCCCACAGTTACGCAAGTCATCGCACAAACCGACGTAATTCGTTACATGCTGACTCGGCCTATGGTCAAAGGCCGAATTGGGAAATGGACACTCGCCTTATCCGAGTTCAGCCTTCAGTATGTACCCCAAAAGGCCGTCAAGGGGCAAGCCCTCGCCGACTTCCTGGCACAACACCCATCCCCTTATGGTTTTGAGGGGGGCGAGGTCGACATCGGTTTCGTCACCACACGCGATAACCATTGGACAATGCATTTTGATGGTTCCAGTACTTCGACCTCGGCAGGTGTCGGCATCGCAATTCAGTCGCCTGACCACTGCCGATGGTGTTTTTCTCTCAAGTTAGATTTCAGCTGCACCAATAATCAGGCCGAGTATGAAGCCCTCATTATCGGCCTCCATGTTTTACACGATCTACGGGCCCCCCGCGTTCTCGTCCTTGGTGATTCCGAACTTGTGATTAACCAACTGAACGGCGTGTTTCGTTGCATGAGTTGTACTTTAGCTCCCTATCACATGGTCGCCACTTACCTGGCCGAGTCGTTCGACCAAATCACATTTGAACACATTTCACGTATCCACAATACTGACGCCGACGAACTCGCCCAGATCGCCTCCGGAGCACAACTCATGGGGGGCAAACTAAGTCAAATTATGGAAATTGTGAACACGGGTCAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

229

Amino Acids

25.23

Weight (kDa)

5.8

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 87 - 204 4.1e-19 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 278
Acc36I ACCTGC 1 cut(s) 278
AccI GTMKAC 1 cut(s) 213
AccII CGCG 2 cut(s) 237, 432
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 537
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 1 cut(s) 594
AfaI GTAC 3 cut(s) 129, 274, 502
AfiI CCNNNNNNNGG 1 cut(s) 147
AgsI TTSAA 1 cut(s) 566
AjnI CCWGG 2 cut(s) 168, 534
AluBI AGCT 2 cut(s) 357, 509
AluI AGCT 2 cut(s) 357, 509
Alw21I GWGCWC 1 cut(s) 628
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 7 cut(s) 62, 76, 138, 372, 397, 423, 537
ApaI GGGCCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 357
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AspS9I GGNCC 2 cut(s) 423, 424
AsuHPI GGTGA 2 cut(s) 220, 458
BaeGI GKGCMC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 1 cut(s) 344
BccI CCATC 3 cut(s) 190, 257, 321
BceAI ACGGC 2 cut(s) 125, 493
BcgI CGANNNNNNTGC 2 cut(s) 291, 325
BciT130I CCWGG 2 cut(s) 170, 536
BciVI GTATCC 1 cut(s) 590
BfuAI ACCTGC 1 cut(s) 278
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 1 cut(s) 358
BlsI GCNGC 1 cut(s) 359
BmcAI AGTACT 1 cut(s) 274
Bme1390I CCNGG 2 cut(s) 170, 536
BmgT120I GGNCC 2 cut(s) 423, 424
BmiI GGNNCC 3 cut(s) 268, 425, 426
BmrFI CCNGG 2 cut(s) 170, 536
BmsI GCATC 1 cut(s) 306
BplI GAGNNNNNCTC 2 cut(s) 615, 647
BpuEI CTTGAG 1 cut(s) 326
BsaAI YACGTR 1 cut(s) 578
BsaHI GRCGYC 1 cut(s) 594
BsaJI CCNNGG 2 cut(s) 282, 442
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 493, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse1I ACTGG 1 cut(s) 270
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 2 cut(s) 170, 536
BseDI CCNNGG 2 cut(s) 282, 442
BseGI GGATG 1 cut(s) 182
BseLI CCNNNNNNNGG 1 cut(s) 147
BseNI ACTGG 1 cut(s) 270
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 344
BsgI GTGCAG 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 237, 432
BshFI GGCC 7 cut(s) 64, 78, 140, 374, 399, 425, 539
BsiHKAI GWGCWC 1 cut(s) 628
BsiSI CCGG 1 cut(s) 621
BslI CCNNNNNNNGG 1 cut(s) 147
BsnI GGCC 7 cut(s) 64, 78, 140, 374, 399, 425, 539
Bsp120I GGGCCC 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 427, 628
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 2 cut(s) 415, 612
BspACI CCGC 1 cut(s) 430
BspANI GGCC 7 cut(s) 64, 78, 140, 374, 399, 425, 539
BspEI TCCGGA 1 cut(s) 620
BspFNI CGCG 2 cut(s) 237, 432
BspLI GGNNCC 3 cut(s) 268, 425, 426
BspMI ACCTGC 1 cut(s) 278
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 2 cut(s) 282, 442
BssMI GATC 2 cut(s) 415, 612
BssNI GRCGYC 1 cut(s) 594
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 170, 536
Bst4CI ACNGT 2 cut(s) 13, 684
BstACI GRCGYC 1 cut(s) 594
BstBAI YACGTR 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 647
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 2 cut(s) 237, 432
BstKTI GATC 2 cut(s) 418, 615
BstMBI GATC 2 cut(s) 415, 612
BstMWI GCNNNNNNNGC 3 cut(s) 61, 614, 623
BstNI CCWGG 2 cut(s) 170, 536
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 168, 534
BstSLI GKGCMC 1 cut(s) 427
BstUI CGCG 2 cut(s) 237, 432
BstV1I GCAGC 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 7 cut(s) 64, 78, 140, 374, 399, 425, 539
BtgZI GCGATG 2 cut(s) 10, 283
BtsCI GGATG 1 cut(s) 182
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 423, 424
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 128, 273, 501
CviAII CATG 5 cut(s) 52, 404, 493, 520, 634
CviQI GTAC 3 cut(s) 128, 273, 501
DdeI CTNAG 1 cut(s) 647
DpnI GATC 2 cut(s) 417, 614
DpnII GATC 2 cut(s) 415, 612
EaeI YGGCCR 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 427
Eco57I CTGAAG 1 cut(s) 104
EcoO109I RGGNCCY 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 168, 534
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 5 cut(s) 55, 407, 496, 523, 637
FatI CATG 5 cut(s) 51, 403, 492, 519, 633
FauI CCCGC 1 cut(s) 437
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 1 cut(s) 358
FokI GGATG 1 cut(s) 169
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 1 cut(s) 358
GluI GCNGC 1 cut(s) 358
HaeIII GGCC 7 cut(s) 64, 78, 140, 374, 399, 425, 539
HapII CCGG 1 cut(s) 621
HgaI GACGC 1 cut(s) 602
Hin1I GRCGYC 1 cut(s) 594
Hin1II CATG 5 cut(s) 55, 407, 496, 523, 637
HincII GTYRAC 2 cut(s) 214, 680
HindII GTYRAC 2 cut(s) 214, 680
HinfI GANTC 3 cut(s) 58, 449, 542
HpaII CCGG 1 cut(s) 621
HphI GGTGA 2 cut(s) 220, 458
Hpy166II GTNNAC 3 cut(s) 214, 670, 680
Hpy188I TCNGA 2 cut(s) 109, 454
Hpy188III TCNNGA 1 cut(s) 621
Hpy8I GTNNAC 3 cut(s) 214, 670, 680
Hpy99I CGWCG 2 cut(s) 41, 602
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 2 cut(s) 13, 684
HpyCH4IV ACGT 2 cut(s) 39, 577
HpyCH4V TGCA 3 cut(s) 256, 360, 492
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 614, 623
HpyF3I CTNAG 1 cut(s) 647
HpySE526I ACGT 2 cut(s) 39, 577
Hsp92I GRCGYC 1 cut(s) 594
Hsp92II CATG 5 cut(s) 55, 407, 496, 523, 637
Kpn2I TCCGGA 1 cut(s) 620
Kzo9I GATC 2 cut(s) 415, 612
LmnI GCTCC 3 cut(s) 9, 514, 623
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 1 cut(s) 306
MaeII ACGT 2 cut(s) 39, 577
MaeIII GTNAC 3 cut(s) 13, 47, 226
MalI GATC 2 cut(s) 417, 614
MboI GATC 2 cut(s) 415, 612
MhlI GDGCHC 2 cut(s) 427, 628
MluCI AATT 5 cut(s) 42, 81, 303, 654, 663
MlyI GAGTC 2 cut(s) 52, 551
MnlI CCTC 7 cut(s) 167, 193, 202, 292, 398, 410, 628
Mph1103I ATGCAT 1 cut(s) 258
MroI TCCGGA 1 cut(s) 620
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 357
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 2 cut(s) 170, 536
MvaI CCWGG 2 cut(s) 170, 536
MvnI CGCG 2 cut(s) 237, 432
MwoI GCNNNNNNNGC 3 cut(s) 61, 614, 623
NdeII GATC 2 cut(s) 415, 612
NlaIII CATG 5 cut(s) 55, 407, 496, 523, 637
NlaIV GGNNCC 3 cut(s) 268, 425, 426
NmeAIII GCCGAG 4 cut(s) 40, 263, 400, 565
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 258
NspI RCATGY 1 cut(s) 55
PaqCI CACCTGC 1 cut(s) 278
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 105
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 1 cut(s) 359
PleI GAGTC 2 cut(s) 52, 550
PpsI GAGTC 2 cut(s) 52, 550
Ppu21I YACGTR 1 cut(s) 578
Psp6I CCWGG 2 cut(s) 168, 534
PspGI CCWGG 2 cut(s) 168, 534
PspN4I GGNNCC 3 cut(s) 268, 425, 426
PspOMI GGGCCC 1 cut(s) 423
PspPI GGNCC 2 cut(s) 423, 424
PvuII CAGCTG 1 cut(s) 357
RsaI GTAC 3 cut(s) 129, 274, 502
RsaNI GTAC 3 cut(s) 128, 273, 501
RseI CAYNNNNRTG 1 cut(s) 261
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 2 cut(s) 415, 612
Sau96I GGNCC 2 cut(s) 423, 424
ScaI AGTACT 1 cut(s) 274
SchI GAGTC 2 cut(s) 52, 551
ScrFI CCNGG 2 cut(s) 170, 536
SduI GDGCHC 2 cut(s) 427, 628
SetI ASST 8 cut(s) 42, 213, 284, 292, 359, 511, 537, 580
SfaNI GCATC 1 cut(s) 306
SmiMI CAYNNNNRTG 1 cut(s) 261
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 5 cut(s) 42, 81, 303, 654, 663
SsiI CCGC 1 cut(s) 430
StyD4I CCNGG 2 cut(s) 168, 534
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 2 cut(s) 13, 684
TaiI ACGT 2 cut(s) 42, 580
TaqI TCGA 3 cut(s) 213, 278, 549
TasI AATT 5 cut(s) 42, 81, 303, 654, 663
TatI WGTACW 2 cut(s) 272, 500
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 326
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 396
TspRI CASTG 1 cut(s) 326
XceI RCATGY 1 cut(s) 55
XmiI GTMKAC 1 cut(s) 213
ZrmI AGTACT 1 cut(s) 274
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.