Rw1G003560

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000160
Physical Location & Seq
Forward (+)
6699379 .. 6701160
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003560.1

Sequence Viewer

Length: 1404 bp
ATGACCAATAACCACTGGACCATGTATTTTGATGGGTCTAGTACTGAATTTTCCGCCGGAGCTGGTGTTGTTATTGAAACGCCTGAAGGACAAAAGTTCCAGTTTGCTTTCCAATTAGATTTTACATGCACAAACAATCAGGCAGAATATGAGGCTCTTATTGTTGGTTTGGAAATTCTCCAAGAGATGGGAGCACGGCGAGTTCTGGTGTTCGGGGATTCTCAACTGGTCATTAACCAAATGAATAAGGAGTTCAAATGCACAAGTTGGGGACTTTTATCATATCACGCTTTAGCCGACCAGCTCGCCAACACGTTCGATCGAATCTCTTTCGCCCACCTCCCACGGGGACAGAACATGGAGGCCAATGAGATAGCCCAATTAGCCTCGGGGTTACGGATCCCCGAAGGAGATGACTCCCGAGTCATCAAGATTGCCAAACGTACCCTCCCAGCTTTGGAAGAGAGAGGAGTCTCAGAAGATGTTTTTGTTATCAACGTCGAGCCAAATGATTGGAGACTCCCCATTATCAAGTTCCACAAAAATCCTCAAGGCAATCATGATCGGAAGACTCGGTATTTGGCTGGACATTTCGTTATATACAAAGAAGCAGTGTACCGCAAAAGCTCCGATGATTTACTCCTTCTTTGTATAGGGGGGCAAGAGACTTTGGAAGTCATGAGAGATGTCCATGAGGGGATATGTGGTGCACACCAGGCCGGAATCAAAATGAGGTGGTTAATTCGAAGACATGGCTTCTATTGGCCAACAATTCTAAAGGATTGCATTGAATTCGCAAAGACCTGCAAGAAATGCCAAATTCATGCACCTGTGCAAAGGGTTCCGGCCGACGTCCTCCATCCAATTGTTAAGCCATGGCCATTTCGAGGTTGGGCCATGGACATTATAGGGAGTATTAGACCACCATCATCAAAACAACACGTATGGATTTTGGTTGCCACTGATTATTTCACTAAATGGGTAGAGGCCAAACCATACGTTACTATTTCAAGTCAGACAGTGATAAAGTTTGTGGAGGAAAACATTGTACACAGGTTTGGGATACCTGAGACCATAACGGCTGATAGGGGTTCGGTTTTCATAGCCGAGGCAATGCATGAACTCACAGATAGTCTGGGTATCAAGTTAACTCACTCGACACCTTACTATGCCCAAGCAAATGGTCAGGCCGAAGCAACCGTTTTGCCTATCGGGACTAAAGATAGACGTTTCGGAAAATGGTCGCCAAGGTGGGAAGGCCCGTTTATCATCGATCAAGTGCTAGGAAAAGGTGCATATCAACTTAGGGATCAAGATGGAGAGCTGCATGCAATGCCTATCAACGGGCAATTTCTTAAGAAATATTACCCCACGACATGGGAGATGAGGGAGCAAGAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

467

Amino Acids

53.23

Weight (kDa)

6.61

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H PF00075 4 - 90 2.6e-07 RNase H
RVT_3 PF13456 10 - 128 7e-19 Reverse transcriptase-like
Integrase_H2C2 PF17921 222 - 276 7.2e-09 Integrase zinc binding domain
rve PF00665 294 - 390 3.2e-14 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AatII GACGTC 1 cut(s) 855
Acc36I ACCTGC 1 cut(s) 812
AccB7I CCANNNNNTGG 2 cut(s) 187, 1377
AciI CCGC 2 cut(s) 54, 619
AclWI GGATC 3 cut(s) 394, 407, 1317
AcoI YGGCCR 3 cut(s) 764, 846, 878
AcsI RAATTY 4 cut(s) 47, 174, 791, 819
AcuI CTGAAG 1 cut(s) 105
AcyI GRCGYC 1 cut(s) 852
AfaI GTAC 4 cut(s) 43, 445, 617, 1050
AfiI CCNNNNNNNGG 6 cut(s) 187, 346, 457, 887, 1343, 1377
AflII CTTAAG 1 cut(s) 1355
AflIII ACRYGT 2 cut(s) 312, 940
AgsI TTSAA 4 cut(s) 77, 256, 791, 1011
AjnI CCWGG 1 cut(s) 714
AluBI AGCT 5 cut(s) 62, 304, 455, 627, 1324
AluI AGCT 5 cut(s) 62, 304, 455, 627, 1324
Alw21I GWGCWC 2 cut(s) 196, 712
Alw26I GTCTC 4 cut(s) 478, 511, 659, 1064
Alw44I GTGCAC 1 cut(s) 708
AlwI GGATC 3 cut(s) 394, 407, 1317
Ama87I CYCGRG 2 cut(s) 388, 420
AoxI GGCC 9 cut(s) 363, 717, 764, 846, 878, 894, 987, 1188, 1258
ApaLI GTGCAC 1 cut(s) 708
ApeKI GCWGC 1 cut(s) 1324
ApoI RAATTY 4 cut(s) 47, 174, 791, 819
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AspS9I GGNCC 3 cut(s) 18, 894, 1259
AsuII TTCGAA 1 cut(s) 745
AvaI CYCGRG 2 cut(s) 388, 420
AvaII GGWCC 1 cut(s) 18
BaeGI GKGCMC 1 cut(s) 712
BalI TGGCCA 2 cut(s) 766, 880
BamHI GGATCC 1 cut(s) 399
BbsI GAAGAC 2 cut(s) 575, 754
Bbv12I GWGCWC 2 cut(s) 196, 712
BbvI GCAGC 1 cut(s) 1311
BccI CCATC 5 cut(s) 26, 181, 867, 934, 1310
BceAI ACGGC 2 cut(s) 212, 1095
BcgI CGANNNNNNTGC 2 cut(s) 775, 809
BciT130I CCWGG 1 cut(s) 716
BciVI GTATCC 1 cut(s) 1056
BcoDI GTCTC 4 cut(s) 478, 511, 659, 1064
BfaI CTAG 2 cut(s) 39, 1283
BfrI CTTAAG 1 cut(s) 1355
BfuAI ACCTGC 1 cut(s) 812
BfuI GTATCC 1 cut(s) 1056
BisI GCNGC 1 cut(s) 1325
BlsI GCNGC 1 cut(s) 1326
BmcAI AGTACT 1 cut(s) 43
Bme1390I CCNGG 1 cut(s) 716
Bme18I GGWCC 1 cut(s) 18
BmeT110I CYCGRG 2 cut(s) 388, 420
BmgT120I GGNCC 3 cut(s) 18, 894, 1259
BmiI GGNNCC 2 cut(s) 401, 843
BmrFI CCNGG 1 cut(s) 716
BpiI GAAGAC 2 cut(s) 575, 754
Bpu14I TTCGAA 1 cut(s) 745
BpuEI CTTGAG 1 cut(s) 534
Bsa29I ATCGAT 1 cut(s) 1272
BsaAI YACGTR 1 cut(s) 943
BsaHI GRCGYC 1 cut(s) 852
BsaI GGTCTC 1 cut(s) 1064
BsaJI CCNNGG 6 cut(s) 344, 387, 875, 897, 1107, 1247
BsaXI ACNNNNNCTCC 4 cut(s) 51, 81, 432, 462
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 346, 457, 887, 1343, 1377
Bse1I ACTGG 3 cut(s) 20, 100, 231
Bse3DI GCAATG 2 cut(s) 1119, 1338
BseBI CCWGG 1 cut(s) 716
BseCI ATCGAT 1 cut(s) 1272
BseDI CCNNGG 6 cut(s) 344, 387, 875, 897, 1107, 1247
BseGI GGATG 1 cut(s) 859
BseLI CCNNNNNNNGG 6 cut(s) 187, 346, 457, 887, 1343, 1377
BseMI GCAATG 2 cut(s) 1119, 1338
BseMII CTCAG 2 cut(s) 489, 1059
BseNI ACTGG 3 cut(s) 20, 100, 231
BseRI GAGGAG 1 cut(s) 483
BseSI GKGCMC 1 cut(s) 712
BseX3I CGGCCG 1 cut(s) 846
BseXI GCAGC 1 cut(s) 1311
BseYI CCCAGC 1 cut(s) 451
Bsh1285I CGRYCG 2 cut(s) 322, 849
BshFI GGCC 9 cut(s) 365, 719, 766, 848, 880, 896, 989, 1190, 1260
BshVI ATCGAT 1 cut(s) 1272
BsiEI CGRYCG 2 cut(s) 322, 849
BsiHKAI GWGCWC 2 cut(s) 196, 712
BsiHKCI CYCGRG 2 cut(s) 388, 420
BsiSI CCGG 3 cut(s) 57, 720, 845
BslFI GGGAC 3 cut(s) 285, 363, 1228
BslI CCNNNNNNNGG 6 cut(s) 187, 346, 457, 887, 1343, 1377
BsmAI GTCTC 4 cut(s) 478, 511, 659, 1064
BsmFI GGGAC 3 cut(s) 285, 363, 1228
BsnI GGCC 9 cut(s) 365, 719, 766, 848, 880, 896, 989, 1190, 1260
Bso31I GGTCTC 1 cut(s) 1064
BsoBI CYCGRG 2 cut(s) 388, 420
Bsp119I TTCGAA 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 196, 712
Bsp1407I TGTACA 1 cut(s) 1048
Bsp143I GATC 5 cut(s) 319, 399, 562, 1273, 1309
Bsp19I CCATGG 2 cut(s) 875, 897
BspACI CCGC 2 cut(s) 54, 619
BspANI GGCC 9 cut(s) 365, 719, 766, 848, 880, 896, 989, 1190, 1260
BspCNI CTCAG 2 cut(s) 488, 1060
BspDI ATCGAT 1 cut(s) 1272
BspHI TCATGA 2 cut(s) 559, 678
BspLI GGNNCC 2 cut(s) 401, 843
BspMI ACCTGC 1 cut(s) 812
BspPI GGATC 3 cut(s) 394, 407, 1317
BspT104I TTCGAA 1 cut(s) 745
BspTI CTTAAG 1 cut(s) 1355
BspTNI GGTCTC 1 cut(s) 1064
BsrDI GCAATG 2 cut(s) 1119, 1338
BsrGI TGTACA 1 cut(s) 1048
BsrI ACTGG 3 cut(s) 20, 100, 231
BssECI CCNNGG 6 cut(s) 344, 387, 875, 897, 1107, 1247
BssMI GATC 5 cut(s) 319, 399, 562, 1273, 1309
BssNI GRCGYC 1 cut(s) 852
BssT1I CCWWGG 3 cut(s) 875, 897, 1247
Bst2UI CCWGG 1 cut(s) 716
Bst4CI ACNGT 2 cut(s) 1021, 1201
Bst6I CTCTTC 1 cut(s) 456
BstACI GRCGYC 1 cut(s) 852
BstAFI CTTAAG 1 cut(s) 1355
BstAPI GCANNNNNTGC 2 cut(s) 813, 1333
BstAUI TGTACA 1 cut(s) 1048
BstBAI YACGTR 1 cut(s) 943
BstBI TTCGAA 1 cut(s) 745
BstC8I GCNNGC 2 cut(s) 306, 1329
BstDEI CTNAG 3 cut(s) 475, 1068, 1304
BstDSI CCRYGG 3 cut(s) 344, 875, 897
BstF5I GGATG 1 cut(s) 859
BstKTI GATC 5 cut(s) 322, 402, 565, 1276, 1312
BstMAI GTCTC 4 cut(s) 478, 511, 659, 1064
BstMBI GATC 5 cut(s) 319, 399, 562, 1273, 1309
BstMCI CGRYCG 2 cut(s) 322, 849
BstMWI GCNNNNNNNGC 4 cut(s) 383, 716, 813, 1333
BstNI CCWGG 1 cut(s) 716
BstNSI RCATGY 2 cut(s) 129, 1331
BstSCI CCNGG 1 cut(s) 714
BstSLI GKGCMC 1 cut(s) 712
BstV1I GCAGC 1 cut(s) 1311
BstV2I GAAGAC 2 cut(s) 575, 754
BstX2I RGATCY 1 cut(s) 399
BstXI CCANNNNNNTGG 2 cut(s) 513, 1181
BstYI RGATCY 1 cut(s) 399
BstZI CGGCCG 1 cut(s) 846
Bsu15I ATCGAT 1 cut(s) 1272
BsuI GTATCC 1 cut(s) 1056
BsuRI GGCC 9 cut(s) 365, 719, 766, 848, 880, 896, 989, 1190, 1260
BsuTUI ATCGAT 1 cut(s) 1272
BtgI CCRYGG 3 cut(s) 344, 875, 897
BtsCI GGATG 1 cut(s) 859
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 4 cut(s) 13, 618, 960, 1026
BveI ACCTGC 1 cut(s) 812
Cac8I GCNNGC 2 cut(s) 306, 1329
CciI TCATGA 2 cut(s) 559, 678
Cfr13I GGNCC 3 cut(s) 18, 894, 1259
ClaI ATCGAT 1 cut(s) 1272
Csp6I GTAC 4 cut(s) 42, 444, 616, 1049
CviQI GTAC 4 cut(s) 42, 444, 616, 1049
DdeI CTNAG 3 cut(s) 475, 1068, 1304
DpnI GATC 5 cut(s) 321, 401, 564, 1275, 1311
DpnII GATC 5 cut(s) 319, 399, 562, 1273, 1309
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
EaeI YGGCCR 3 cut(s) 764, 846, 878
EagI CGGCCG 1 cut(s) 846
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
EciI GGCGGA 1 cut(s) 43
EclXI CGGCCG 1 cut(s) 846
Eco130I CCWWGG 3 cut(s) 875, 897, 1247
Eco31I GGTCTC 1 cut(s) 1064
Eco47I GGWCC 1 cut(s) 18
Eco52I CGGCCG 1 cut(s) 846
Eco57I CTGAAG 1 cut(s) 105
Eco88I CYCGRG 2 cut(s) 388, 420
EcoRI GAATTC 1 cut(s) 791
EcoRII CCWGG 1 cut(s) 714
EcoT14I CCWWGG 3 cut(s) 875, 897, 1247
EcoT22I ATGCAT 1 cut(s) 1119
ErhI CCWWGG 3 cut(s) 875, 897, 1247
FaqI GGGAC 3 cut(s) 285, 363, 1228
Fnu4HI GCNGC 1 cut(s) 1325
FokI GGATG 1 cut(s) 846
Fsp4HI GCNGC 1 cut(s) 1325
FspBI CTAG 2 cut(s) 39, 1283
GluI GCNGC 1 cut(s) 1325
GsaI CCCAGC 1 cut(s) 455
HaeIII GGCC 9 cut(s) 365, 719, 766, 848, 880, 896, 989, 1190, 1260
HapII CCGG 3 cut(s) 57, 720, 845
Hin1I GRCGYC 1 cut(s) 852
HincII GTYRAC 1 cut(s) 1149
HindII GTYRAC 1 cut(s) 1149
HinfI GANTC 9 cut(s) 218, 324, 416, 423, 471, 519, 571, 723, 1397
HpaI GTTAAC 1 cut(s) 1149
HpaII CCGG 3 cut(s) 57, 720, 845
Hpy166II GTNNAC 4 cut(s) 616, 710, 1051, 1149
Hpy188I TCNGA 5 cut(s) 478, 567, 631, 1017, 1235
Hpy188III TCNNGA 6 cut(s) 420, 430, 560, 679, 1213, 1313
Hpy8I GTNNAC 4 cut(s) 616, 710, 1051, 1149
Hpy99I CGWCG 2 cut(s) 503, 854
HpyAV CCTTC 4 cut(s) 80, 401, 653, 1250
HpyCH4III ACNGT 2 cut(s) 1021, 1201
HpyCH4IV ACGT 7 cut(s) 314, 442, 498, 852, 942, 999, 1228
HpyF10VI GCNNNNNNNGC 4 cut(s) 383, 716, 813, 1333
HpyF3I CTNAG 3 cut(s) 475, 1068, 1304
HpySE526I ACGT 7 cut(s) 314, 442, 498, 852, 942, 999, 1228
Hsp92I GRCGYC 1 cut(s) 852
KspAI GTTAAC 1 cut(s) 1149
Kzo9I GATC 5 cut(s) 319, 399, 562, 1273, 1309
LmnI GCTCC 4 cut(s) 59, 191, 632, 1390
Lsp1109I GCAGC 1 cut(s) 1311
MaeI CTAG 2 cut(s) 39, 1283
MaeII ACGT 7 cut(s) 314, 442, 498, 852, 942, 999, 1228
MaeIII GTNAC 2 cut(s) 393, 1000
MalI GATC 5 cut(s) 321, 401, 564, 1275, 1311
MboI GATC 5 cut(s) 319, 399, 562, 1273, 1309
MboII GAAGA 4 cut(s) 473, 491, 580, 759
MfeI CAATTG 1 cut(s) 864
MflI RGATCY 1 cut(s) 399
MhlI GDGCHC 2 cut(s) 196, 712
MlsI TGGCCA 2 cut(s) 766, 880
MluNI TGGCCA 2 cut(s) 766, 880
MlyI GAGTC 5 cut(s) 410, 432, 480, 513, 565
Mox20I TGGCCA 2 cut(s) 766, 880
Mph1103I ATGCAT 1 cut(s) 1119
MscI TGGCCA 2 cut(s) 766, 880
MseI TTAA 5 cut(s) 234, 740, 870, 1148, 1356
Msp20I TGGCCA 2 cut(s) 766, 880
MspCI CTTAAG 1 cut(s) 1355
MspI CCGG 3 cut(s) 57, 720, 845
MspR9I CCNGG 1 cut(s) 716
MunI CAATTG 1 cut(s) 864
MvaI CCWGG 1 cut(s) 716
MwoI GCNNNNNNNGC 4 cut(s) 383, 716, 813, 1333
NcoI CCATGG 2 cut(s) 875, 897
NdeII GATC 5 cut(s) 319, 399, 562, 1273, 1309
NlaIV GGNNCC 2 cut(s) 401, 843
NmeAIII GCCGAG 1 cut(s) 1132
NsiI ATGCAT 1 cut(s) 1119
NspI RCATGY 2 cut(s) 129, 1331
NspV TTCGAA 1 cut(s) 745
PaeI GCATGC 1 cut(s) 1331
PagI TCATGA 2 cut(s) 559, 678
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PfeI GAWTC 4 cut(s) 218, 324, 723, 1397
PflMI CCANNNNNTGG 2 cut(s) 187, 1377
PkrI GCNGC 1 cut(s) 1326
Ple19I CGATCG 1 cut(s) 322
PleI GAGTC 5 cut(s) 410, 431, 479, 513, 565
PpsI GAGTC 5 cut(s) 410, 431, 479, 513, 565
Ppu21I YACGTR 1 cut(s) 943
Psp6I CCWGG 1 cut(s) 714
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 1 cut(s) 714
PspN4I GGNNCC 2 cut(s) 401, 843
PspPI GGNCC 3 cut(s) 18, 894, 1259
PsuI RGATCY 1 cut(s) 399
PvuI CGATCG 1 cut(s) 322
RsaI GTAC 4 cut(s) 43, 445, 617, 1050
RsaNI GTAC 4 cut(s) 42, 444, 616, 1049
SaqAI TTAA 5 cut(s) 234, 740, 870, 1148, 1356
SatI GCNGC 1 cut(s) 1325
Sau3AI GATC 5 cut(s) 319, 399, 562, 1273, 1309
Sau96I GGNCC 3 cut(s) 18, 894, 1259
ScaI AGTACT 1 cut(s) 43
SchI GAGTC 5 cut(s) 410, 432, 480, 513, 565
ScrFI CCNGG 1 cut(s) 716
SduI GDGCHC 2 cut(s) 196, 712
SfuI TTCGAA 1 cut(s) 745
SinI GGWCC 1 cut(s) 18
SmlI CTYRAG 2 cut(s) 549, 1355
SmoI CTYRAG 2 cut(s) 549, 1355
SphI GCATGC 1 cut(s) 1331
SsiI CCGC 2 cut(s) 54, 619
SspI AATATT 1 cut(s) 1364
SspMI CTAG 2 cut(s) 39, 1283
StyD4I CCNGG 1 cut(s) 714
StyI CCWWGG 3 cut(s) 875, 897, 1247
TaaI ACNGT 2 cut(s) 1021, 1201
TaiI ACGT 7 cut(s) 317, 445, 501, 855, 945, 1002, 1231
TaqI TCGA 7 cut(s) 318, 322, 501, 745, 886, 1157, 1272
TatI WGTACW 2 cut(s) 41, 1048
TfiI GAWTC 4 cut(s) 218, 324, 723, 1397
Tru1I TTAA 5 cut(s) 234, 740, 870, 1148, 1356
Tru9I TTAA 5 cut(s) 234, 740, 870, 1148, 1356
TscAI CASTG 4 cut(s) 20, 618, 967, 1026
TseI GCWGC 1 cut(s) 1324
TspDTI ATGAA 4 cut(s) 257, 812, 1090, 1134
TspGWI ACGGA 1 cut(s) 412
TspRI CASTG 4 cut(s) 20, 618, 967, 1026
Van91I CCANNNNNTGG 2 cut(s) 187, 1377
Vha464I CTTAAG 1 cut(s) 1355
VneI GTGCAC 1 cut(s) 708
VpaK11BI GGWCC 1 cut(s) 18
XapI RAATTY 4 cut(s) 47, 174, 791, 819
XceI RCATGY 2 cut(s) 129, 1331
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XspI CTAG 2 cut(s) 39, 1283
ZraI GACGTC 1 cut(s) 853
ZrmI AGTACT 1 cut(s) 43
Zsp2I ATGCAT 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.