Rroxscaffold_3G00223200

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000160
Physical Location & Seq
Forward (+)
6312814 .. 6313371
558 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00223200.1

Sequence Viewer

Length: 558 bp
ATGTACAAACAATCAAGCGAGTACGAGGCCCTTATCATAGGTTTGGAGGTATTGCGGAATTGGGAATCGAGACGTTCGGTGTGTGGCGACTCTCTACTCGTGATAAATCAACCGCAAAAGACGTACGGGTGCGAAAGCCGTTTGCTCGTACCTTATCTAAATCGCGCTATTGAGCTTCTTGACCAATTTGACGACGTGGATTTGGAGTTTATTCCTCGCGAGCACAATTACGTGGCCAATGAGCTCGCTCATCCGGCTACGAGCATTACGTTAAAGTATGGGGTCCGCGAGTGCATCTTGAAAGTCGAGCGCCACACGTTGTCTTCTCGGCTCGCACGACCCGACCCGCCCGATGATCCCGTCGTCGCAGTCCTAGAACCTATTGACGTCGATCGGCGCATTCCGTTGATCGCTTACCTCGTACAACCGGATCCTACGGCGGACGGGAAGATTCGTTTTTTGGCCTTAATTTACTTCCTCGGGGAGATGAGCTGCGGCGACGCGGGGAAGATGGCATGGACTTCCGATGTGTCTATGGCCGAGAAGCCAAATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

185

Amino Acids

20.91

Weight (kDa)

4.92

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 4 - 84 2.1e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 390
AccII CGCG 4 cut(s) 165, 219, 288, 503
AciI CCGC 7 cut(s) 55, 113, 286, 347, 440, 495, 503
AclWI GGATC 3 cut(s) 350, 425, 438
AcoI YGGCCR 2 cut(s) 234, 537
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 5 cut(s) 5, 23, 125, 150, 423
AflIII ACRYGT 1 cut(s) 315
AgsI TTSAA 1 cut(s) 301
AjiI CACGTC 1 cut(s) 196
AluBI AGCT 3 cut(s) 175, 244, 492
AluI AGCT 3 cut(s) 175, 244, 492
Alw21I GWGCWC 2 cut(s) 225, 246
Alw26I GTCTC 1 cut(s) 64
AlwI GGATC 3 cut(s) 350, 425, 438
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 4 cut(s) 27, 234, 462, 537
ApeKI GCWGC 1 cut(s) 492
AspLEI GCGC 3 cut(s) 167, 312, 399
AspS9I GGNCC 2 cut(s) 28, 283
AvaI CYCGRG 1 cut(s) 479
AvaII GGWCC 1 cut(s) 283
BalI TGGCCA 1 cut(s) 236
BamHI GGATCC 1 cut(s) 430
BanII GRGCYC 1 cut(s) 246
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 2 cut(s) 225, 246
BbvI GCAGC 1 cut(s) 479
BccI CCATC 1 cut(s) 505
BceAI ACGGC 2 cut(s) 123, 453
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 313
BisI GCNGC 2 cut(s) 493, 496
BlsI GCNGC 2 cut(s) 494, 497
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 479
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 28, 283
BmiI GGNNCC 2 cut(s) 284, 432
BmsI GCATC 1 cut(s) 303
BpiI GAAGAC 1 cut(s) 315
BsaAI YACGTR 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 387
BsaJI CCNNGG 1 cut(s) 478
BsaWI WCCGGW 1 cut(s) 427
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 1 cut(s) 250
BseXI GCAGC 1 cut(s) 479
Bsh1236I CGCG 4 cut(s) 165, 219, 288, 503
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 4 cut(s) 29, 236, 464, 539
BsiEI CGRYCG 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 225, 246
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 2 cut(s) 254, 428
BsiWI CGTACG 1 cut(s) 123
BsmAI GTCTC 1 cut(s) 64
BsmBI CGTCTC 1 cut(s) 64
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 4 cut(s) 29, 236, 464, 539
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 2 cut(s) 225, 246
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 355, 391, 408, 430
Bsp68I TCGCGA 1 cut(s) 219
BspACI CCGC 7 cut(s) 55, 113, 286, 347, 440, 495, 503
BspANI GGCC 4 cut(s) 29, 236, 464, 539
BspFNI CGCG 4 cut(s) 165, 219, 288, 503
BspLI GGNNCC 2 cut(s) 284, 432
BspPI GGATC 3 cut(s) 350, 425, 438
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 4 cut(s) 355, 391, 408, 430
BssNI GRCGYC 1 cut(s) 387
BssSI CACGAG 1 cut(s) 98
Bst2BI CACGAG 1 cut(s) 98
BstACI GRCGYC 1 cut(s) 387
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 3 cut(s) 221, 246, 333
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 4 cut(s) 165, 219, 288, 503
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 3 cut(s) 167, 312, 399
BstKTI GATC 4 cut(s) 358, 394, 411, 433
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 4 cut(s) 355, 391, 408, 430
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstUI CGCG 4 cut(s) 165, 219, 288, 503
BstV1I GCAGC 1 cut(s) 479
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BsuRI GGCC 4 cut(s) 29, 236, 464, 539
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 1 cut(s) 250
BtuMI TCGCGA 1 cut(s) 219
Cac8I GCNNGC 3 cut(s) 221, 246, 333
CfoI GCGC 3 cut(s) 167, 312, 399
Cfr13I GGNCC 2 cut(s) 28, 283
CseI GACGC 1 cut(s) 509
Csp6I GTAC 5 cut(s) 4, 22, 124, 149, 422
CviAII CATG 1 cut(s) 516
CviQI GTAC 5 cut(s) 4, 22, 124, 149, 422
DpnI GATC 4 cut(s) 357, 393, 410, 432
DpnII GATC 4 cut(s) 355, 391, 408, 430
EaeI YGGCCR 2 cut(s) 234, 537
EciI GGCGGA 1 cut(s) 455
Ecl136II GAGCTC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 479
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 28
EcoT38I GRGCYC 1 cut(s) 246
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 519
FaiI YATR 4 cut(s) 38, 279, 517, 536
FatI CATG 1 cut(s) 515
FauI CCCGC 2 cut(s) 354, 496
Fnu4HI GCNGC 2 cut(s) 493, 496
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 2 cut(s) 493, 496
FspBI CTAG 1 cut(s) 374
GlaI GCGC 3 cut(s) 166, 311, 398
GluI GCNGC 2 cut(s) 493, 496
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 4 cut(s) 29, 236, 464, 539
HapII CCGG 2 cut(s) 254, 428
HgaI GACGC 1 cut(s) 509
HhaI GCGC 3 cut(s) 167, 312, 399
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 1 cut(s) 519
Hin6I GCGC 3 cut(s) 165, 310, 397
HinP1I GCGC 3 cut(s) 165, 310, 397
HinfI GANTC 3 cut(s) 65, 89, 451
HpaII CCGG 2 cut(s) 254, 428
Hpy188I TCNGA 1 cut(s) 526
Hpy188III TCNNGA 5 cut(s) 69, 100, 179, 218, 298
Hpy99I CGWCG 5 cut(s) 197, 365, 368, 392, 503
HpyCH4IV ACGT 7 cut(s) 73, 122, 195, 231, 269, 317, 387
HpyCH4V TGCA 1 cut(s) 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpySE526I ACGT 7 cut(s) 73, 122, 195, 231, 269, 317, 387
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 1 cut(s) 519
HspAI GCGC 3 cut(s) 165, 310, 397
Kzo9I GATC 4 cut(s) 355, 391, 408, 430
LpnPI CCDG 2 cut(s) 267, 441
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 1 cut(s) 303
MaeI CTAG 1 cut(s) 374
MaeII ACGT 7 cut(s) 73, 122, 195, 231, 269, 317, 387
MalI GATC 4 cut(s) 357, 393, 410, 432
MboI GATC 4 cut(s) 355, 391, 408, 430
MboII GAAGA 3 cut(s) 315, 460, 520
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 2 cut(s) 225, 246
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 4 cut(s) 58, 185, 226, 468
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 1 cut(s) 83
MnlI CCTC 5 cut(s) 19, 40, 225, 428, 488
Mox20I TGGCCA 1 cut(s) 236
MscI TGGCCA 1 cut(s) 236
MseI TTAA 3 cut(s) 272, 467, 556
Msp20I TGGCCA 1 cut(s) 236
MspI CCGG 2 cut(s) 254, 428
Mva1269I GAATGC 1 cut(s) 399
MvnI CGCG 4 cut(s) 165, 219, 288, 503
MwoI GCNNNNNNNGC 1 cut(s) 254
NdeII GATC 4 cut(s) 355, 391, 408, 430
NlaIII CATG 1 cut(s) 519
NlaIV GGNNCC 2 cut(s) 284, 432
NmeAIII GCCGAG 1 cut(s) 307
NruI TCGCGA 1 cut(s) 219
PcsI WCGNNNNNNNCGW 4 cut(s) 266, 334, 339, 417
PctI GAATGC 1 cut(s) 399
PfeI GAWTC 2 cut(s) 65, 451
Pfl23II CGTACG 1 cut(s) 123
PkrI GCNGC 2 cut(s) 494, 497
Ple19I CGATCG 1 cut(s) 394
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 246
PspLI CGTACG 1 cut(s) 123
PspN4I GGNNCC 2 cut(s) 284, 432
PspPI GGNCC 2 cut(s) 28, 283
PsuI RGATCY 1 cut(s) 430
PvuI CGATCG 1 cut(s) 394
RruI TCGCGA 1 cut(s) 219
RsaI GTAC 5 cut(s) 5, 23, 125, 150, 423
RsaNI GTAC 5 cut(s) 4, 22, 124, 149, 422
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 3 cut(s) 272, 467, 556
SatI GCNGC 2 cut(s) 493, 496
Sau3AI GATC 4 cut(s) 355, 391, 408, 430
Sau96I GGNCC 2 cut(s) 28, 283
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 2 cut(s) 225, 246
SfaNI GCATC 1 cut(s) 303
SinI GGWCC 1 cut(s) 283
Sse9I AATT 4 cut(s) 58, 185, 226, 468
SsiI CCGC 7 cut(s) 55, 113, 286, 347, 440, 495, 503
SspMI CTAG 1 cut(s) 374
SstI GAGCTC 1 cut(s) 246
TaiI ACGT 7 cut(s) 76, 125, 198, 234, 272, 320, 390
TaqI TCGA 3 cut(s) 68, 306, 390
TasI AATT 4 cut(s) 58, 185, 226, 468
TatI WGTACW 1 cut(s) 3
TauI GCSGC 1 cut(s) 498
TfiI GAWTC 2 cut(s) 65, 451
Tru1I TTAA 3 cut(s) 272, 467, 556
Tru9I TTAA 3 cut(s) 272, 467, 556
TseI GCWGC 1 cut(s) 492
TspGWI ACGGA 1 cut(s) 393
VpaK11BI GGWCC 1 cut(s) 283
XspI CTAG 1 cut(s) 374
ZraI GACGTC 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.