Rw6G033800

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000160
Physical Location & Seq
Reverse (-)
58394840 .. 58398314
3475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033800.1

Sequence Viewer

Length: 2850 bp
ATGAGGAAACTGTTGGCTCATTGGGCCGAAAACTCTCTTCAGAATGATTCGGCCATGAATTTGCTGGAATTTATCACAGAAGGAGCACCAGAGGATGAACTAAAGCTAGAAGACATCGGCCCTGCACCGGCCGAGTTGGAAGACGACCTTACTGAAACTCAAGACCCACTAGAAGACTTGAATTTAGGGACCACCGAGGAGCCTCGGATAGTTAGGATCAGCAAGCTCCTACCACCCAAGTTGAAGGAAGACTTGAAGGCTCTGCTGACTGAGTTTCAAGATTGTTTTGCATGGAGTTACCATGAAATGCCTGGTTTAGATAGAAGTATAGTAGAACATAGGTTGCCTATACAATCCAATTGTAAGCCTTACAAGCAACCTCCTCGGCGATGTGCCGCCGAGGTTTTGCCTGAAATTAAAGAAGAAATGGAACGTTTATTAAAGGCCGGTTTTATTCGAACGGCCAGGTATGTTGAATGGTTATCTAATATTGTGCCAGTAAGGAAAAAGAATGGCAAAATGCGCATATGTGTTGATTTTAGAAATCTAAACTTGGCCACACCCAAAGATGAGTACCCAATGCCCGTGGCCGACCTGCTCGTGGATGGTGCGGCCAAACATGAAATTCTTTCTTTCATGGACGGGCACGCTGGCTACAACCAAATTTTTATAGCTGAGGAGGATGTTCACAAAACGGCGTTTAGATGCCCAGGCGCAATAGGAACATTTGAGTGGCTCATTCAGCCATTCTCTTCACTTTTGAGAACCAAGGCCGACGATGAATTTGTGTGGAGCACAGAGCACCAAGCGGCATTCGACCAACTCAAACGATACTTATCAAACCCTCCCGTCTTGGTACCCCCAGTACGTGGGAGACCTTTGAAGTTATACATATCAGCATCGGATAACTCAATAGGAAGCCTACTAGCACAAGATAACGACAATGGCAAGGAATGTGCTGTTCATTACTTGAGCCGGATCCTCACCGATGTGGAAACTCGGTACACGCCTATTGAGAGGCTTTGTCTTGCATTGTTCTTTTCAGCCATCAGGCTTCGGCACTACATGTTACCCTCGGTCGTCCAAGTCATATCCAAGACCGATTTAGTAAAGTACATGCTGACTCGGCCCATCATACGAGGCCGAATTGGAAAATGGACCATGGCGTTGTCTGAGTTTACGTTTCAATATGTGTCTCAAAAGTCCGTCAAGGGCCAAGCATTGGCCGATTTTCTTGCTCATCACCCTTCGACCGAGTTTGAGGGAGCCGAGGAGGTTGATATTGGGATCATATATGTGGCTTCCATGACCAATAACCACTGGACCATGTATTTTGATGGGTCTAGTACTGAATTTTCCGCTGGAGCTGGTGTTGTTATTGAAACGCCTGAAGGACAAAAGTTCCAGTTTGCTTTCCAATTAGATTTTACATGCACAAACAATCAAGCAGAATATGAGGCTCTTATTGTTGGTTTGGAGATTCTCCAAGAGATGGGAGCACGGCGAGTTCTGGTGTTCGGGGATTCTCAACTGGTCATTAACCAAATGAATAAGGAGTTCAGATGCACAAGCTGGGGACTTTTATCATATCACGCTTTAGCCGACCAGCTTGCCAACACGTTCGATCAAATCTCTTTCGCCCATCTCCCACGGGGACAGAACATGGAGGCCAATGAGATGGCCCAATTAGCCTCGGGGTTACGGATCCCCGAAGGAGATGACTCCCGAGTCATCAAGATTGCCAAACGTACCCTCCCAGCTTTGGAAGAGAGAGGAGTCTCAGAAGATGTTTTTGTTATCGACGTCGAGCCAAATGATTGGAGACTCCCCATTATCAAGTTCCACAAAAATCCTCAAGGCAATCATGATCGAAAGACTCGGTACTTGGCTGGACATTTCGTTATATACAAAGAAGCAGTGTACCGCAAAAGCTCCGATGATCTACTCCTTCTTTGTATAGGGGGGCAAGAGACTTTGGAAGTCATGAGAGATGTCCATGAGGGGATTTGTGGTGCACACCAGGCCGGAATCAAAATGAGGTGGTTAATTCGAAGACATGGCTTCTATTGGCCAACAATTCTAAAGGATTGCATCGAATTCGCAAAGACCTGCAAGAAATGCCAAATTCATGCACCTGTGCAAAGGGTTCCGGCTGACGTCCTCCATCCAATTGTTAAGCCATGGCCATTTCGAGGTTGGGCCATGGACATTATAGGGAGTATTAGACCACCATCATCAAAACAACACGTATGGATTTTGGTTGCCACTGATTATTTCACTAAATGGGTAGAGGCCAAACCATACGTTACTATTTCAAGTCAGACAGTGATAAAGTTTGTGGAGGAAAACATTGTACACAGGTTTGGGATACCTGAGACCATAACGGCCGATAGGGGTTCGGTTTTCATAGCCGAGGCAATGCATGAACTCACAGATAGTCTGGGTATCAAGTTAACTCACTCGACACCTTACTATGCCCAAGCAAATGGTCAGGCCGAAGCAATGATTCAGGAACTTGAAGAACTCGACCAAGAGCGAATGGACGCTTACAACCGAATGGAAGCACAGAAAAAGGCTGTGGCTCGTGCATACAACAAGCGAGTTAAGTCTAAGTCATTTGCCGAGGAAGATTTAGTTTGGAAAGCCGTTTTGCCTATCGGGACTAAAGATAGACGTTTCGGAAAATGGTCGCCAAGGTGGGAAGGCCCGTTTATCATCGATCAAGTGCTAGGAAAAGGTGCATATCAACTTAGGGATCAAGATGGAGAGCTGCACGCAATGCCTATCAACGGGCAATTTCTTAAGAAATATTACCCCACGACATGGGAGATGAGGGAGCAAGAATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

949

Amino Acids

108.36

Weight (kDa)

5.94

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH_2 PF17919 264 - 359 2.5e-22 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 292 - 393 5.1e-18 RNase H-like domain found in reverse transcriptase
RNase_H PF00075 439 - 528 5.7e-07 RNase H
RVT_3 PF13456 445 - 563 2.8e-18 Reverse transcriptase-like
Integrase_H2C2 PF17921 657 - 711 1.8e-08 Integrase zinc binding domain
rve PF00665 729 - 825 9.3e-14 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1727
AatII GACGTC 2 cut(s) 1806, 2160
Acc16I TGCGCA 1 cut(s) 524
Acc36I ACCTGC 2 cut(s) 603, 2117
Acc65I GGTACC 1 cut(s) 856
AccB1I GGYRCC 1 cut(s) 856
AccB7I CCANNNNNTGG 4 cut(s) 869, 1222, 1492, 2823
AciI CCGC 5 cut(s) 396, 611, 809, 1359, 1924
AclI AACGTT 1 cut(s) 433
AclWI GGATC 7 cut(s) 224, 973, 986, 1295, 1699, 1712, 2763
AcsI RAATTY 9 cut(s) 58, 68, 181, 624, 663, 782, 1352, 2096, 2124
AcuI CTGAAG 2 cut(s) 23, 1410
AcyI GRCGYC 2 cut(s) 1803, 2157
AfiI CCNNNNNNNGG 9 cut(s) 127, 601, 869, 1222, 1492, 1762, 2192, 2789, 2823
AflII CTTAAG 1 cut(s) 2801
AflIII ACRYGT 3 cut(s) 1065, 1617, 2245
AjnI CCWGG 4 cut(s) 310, 464, 709, 2019
AleI CACNNNNGTG 1 cut(s) 989
AluBI AGCT 9 cut(s) 106, 226, 674, 1367, 1572, 1609, 1760, 1932, 2770
AluI AGCT 9 cut(s) 106, 226, 674, 1367, 1572, 1609, 1760, 1932, 2770
Alw21I GWGCWC 5 cut(s) 88, 797, 804, 1501, 2017
Alw26I GTCTC 6 cut(s) 868, 1200, 1783, 1816, 1964, 2369
Alw44I GTGCAC 1 cut(s) 2013
AlwI GGATC 7 cut(s) 224, 973, 986, 1295, 1699, 1712, 2763
Ama87I CYCGRG 2 cut(s) 1693, 1725
ApaLI GTGCAC 1 cut(s) 2013
ApeKI GCWGC 1 cut(s) 2770
ApoI RAATTY 9 cut(s) 58, 68, 181, 624, 663, 782, 1352, 2096, 2124
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
Asp718I GGTACC 1 cut(s) 856
AspLEI GCGC 2 cut(s) 525, 716
AsuHPI GGTGA 2 cut(s) 976, 1235
AsuII TTCGAA 2 cut(s) 457, 2050
AvaI CYCGRG 2 cut(s) 1693, 1725
AvaII GGWCC 3 cut(s) 189, 1158, 1323
BaeGI GKGCMC 2 cut(s) 648, 2017
BalI TGGCCA 3 cut(s) 557, 2071, 2185
BamHI GGATCC 2 cut(s) 978, 1704
BanI GGYRCC 1 cut(s) 856
BauI CACGAG 2 cut(s) 599, 2583
BbsI GAAGAC 5 cut(s) 117, 147, 180, 255, 2059
Bbv12I GWGCWC 5 cut(s) 88, 797, 804, 1501, 2017
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 1 cut(s) 2757
BceAI ACGGC 5 cut(s) 477, 711, 1517, 2400, 2630
BcgI CGANNNNNNTGC 8 cut(s) 365, 399, 1217, 1251, 1592, 1626, 2080, 2114
BciT130I CCWGG 4 cut(s) 312, 466, 711, 2021
BciVI GTATCC 1 cut(s) 2361
BcoDI GTCTC 6 cut(s) 868, 1200, 1783, 1816, 1964, 2369
BfaI CTAG 5 cut(s) 107, 170, 926, 1344, 2729
BfrI CTTAAG 1 cut(s) 2801
BfuAI ACCTGC 2 cut(s) 603, 2117
BfuI GTATCC 1 cut(s) 2361
BisI GCNGC 4 cut(s) 396, 612, 810, 2771
BlsI GCNGC 4 cut(s) 397, 613, 811, 2772
BmcAI AGTACT 1 cut(s) 1348
Bme1390I CCNGG 4 cut(s) 312, 466, 711, 2021
Bme18I GGWCC 3 cut(s) 189, 1158, 1323
BmeT110I CYCGRG 2 cut(s) 1693, 1725
BmiI GGNNCC 7 cut(s) 190, 201, 858, 980, 1267, 1706, 2148
BmrFI CCNGG 4 cut(s) 312, 466, 711, 2021
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 4 cut(s) 695, 908, 1553, 2100
BmuI ACTGGG 1 cut(s) 857
BpiI GAAGAC 5 cut(s) 117, 147, 180, 255, 2059
BpmI CTGGAG 1 cut(s) 1383
Bpu10I CCTNAGC 1 cut(s) 675
Bpu14I TTCGAA 2 cut(s) 457, 2050
BpuEI CTTGAG 3 cut(s) 144, 991, 1839
Bsa29I ATCGAT 1 cut(s) 2718
BsaAI YACGTR 2 cut(s) 869, 2248
BsaBI GATNNNNATC 1 cut(s) 835
BsaHI GRCGYC 2 cut(s) 1803, 2157
BsaI GGTCTC 2 cut(s) 868, 2369
BsaXI ACNNNNNCTCC 4 cut(s) 1356, 1386, 1737, 1767
Bsc4I CCNNNNNNNGG 9 cut(s) 127, 601, 869, 1222, 1492, 1762, 2192, 2789, 2823
Bse118I RCCGGY 2 cut(s) 127, 446
Bse1I ACTGG 5 cut(s) 497, 863, 1325, 1405, 1536
Bse3DI GCAATG 3 cut(s) 2424, 2508, 2784
Bse8I GATNNNNATC 1 cut(s) 835
BseBI CCWGG 4 cut(s) 312, 466, 711, 2021
BseCI ATCGAT 1 cut(s) 2718
BseGI GGATG 4 cut(s) 100, 610, 688, 2164
BseJI GATNNNNATC 1 cut(s) 835
BseLI CCNNNNNNNGG 9 cut(s) 127, 601, 869, 1222, 1492, 1762, 2192, 2789, 2823
BseMI GCAATG 3 cut(s) 2424, 2508, 2784
BseMII CTCAG 5 cut(s) 261, 666, 1164, 1794, 2364
BseNI ACTGG 5 cut(s) 497, 863, 1325, 1405, 1536
BseRI GAGGAG 5 cut(s) 212, 372, 692, 1286, 1788
BseSI GKGCMC 2 cut(s) 648, 2017
BseX3I CGGCCG 2 cut(s) 129, 2385
BseXI GCAGC 1 cut(s) 2757
BseYI CCCAGC 2 cut(s) 1572, 1756
BsgI GTGCAG 2 cut(s) 108, 2756
Bsh1285I CGRYCG 4 cut(s) 132, 1080, 1254, 2388
BshNI GGYRCC 1 cut(s) 856
BshVI ATCGAT 1 cut(s) 2718
BsiEI CGRYCG 4 cut(s) 132, 1080, 1254, 2388
BsiHKAI GWGCWC 5 cut(s) 88, 797, 804, 1501, 2017
BsiHKCI CYCGRG 2 cut(s) 1693, 1725
BsiSI CCGG 5 cut(s) 128, 447, 976, 2025, 2150
BslFI GGGAC 4 cut(s) 202, 1590, 1668, 2674
BslI CCNNNNNNNGG 9 cut(s) 127, 601, 869, 1222, 1492, 1762, 2192, 2789, 2823
BsmAI GTCTC 6 cut(s) 868, 1200, 1783, 1816, 1964, 2369
BsmFI GGGAC 4 cut(s) 202, 1590, 1668, 2674
BsmI GAATGC 1 cut(s) 812
Bso31I GGTCTC 2 cut(s) 868, 2369
BsoBI CYCGRG 2 cut(s) 1693, 1725
Bsp119I TTCGAA 2 cut(s) 457, 2050
Bsp1286I GDGCHC 6 cut(s) 88, 648, 797, 804, 1501, 2017
Bsp1407I TGTACA 1 cut(s) 2353
Bsp143I GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
Bsp19I CCATGG 3 cut(s) 1161, 2180, 2202
BspACI CCGC 5 cut(s) 396, 611, 809, 1359, 1924
BspCNI CTCAG 5 cut(s) 262, 667, 1165, 1793, 2365
BspDI ATCGAT 1 cut(s) 2718
BspHI TCATGA 2 cut(s) 1864, 1983
BspLI GGNNCC 7 cut(s) 190, 201, 858, 980, 1267, 1706, 2148
BspMI ACCTGC 2 cut(s) 603, 2117
BspPI GGATC 7 cut(s) 224, 973, 986, 1295, 1699, 1712, 2763
BspT104I TTCGAA 2 cut(s) 457, 2050
BspT107I GGYRCC 1 cut(s) 856
BspTI CTTAAG 1 cut(s) 2801
BspTNI GGTCTC 2 cut(s) 868, 2369
BsrDI GCAATG 3 cut(s) 2424, 2508, 2784
BsrFI RCCGGY 2 cut(s) 127, 446
BsrGI TGTACA 1 cut(s) 2353
BsrI ACTGG 5 cut(s) 497, 863, 1325, 1405, 1536
BssAI RCCGGY 2 cut(s) 127, 446
BssMI GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
BssNI GRCGYC 2 cut(s) 1803, 2157
BssSI CACGAG 2 cut(s) 599, 2583
BssT1I CCWWGG 5 cut(s) 768, 1161, 2180, 2202, 2693
Bst2BI CACGAG 2 cut(s) 599, 2583
Bst2UI CCWGG 4 cut(s) 312, 466, 711, 2021
Bst4CI ACNGT 2 cut(s) 12, 2326
Bst6I CTCTTC 3 cut(s) 42, 757, 1761
BstACI GRCGYC 2 cut(s) 1803, 2157
BstAFI CTTAAG 1 cut(s) 2801
BstAPI GCANNNNNTGC 2 cut(s) 2118, 2779
BstAUI TGTACA 1 cut(s) 2353
BstBAI YACGTR 2 cut(s) 869, 2248
BstBI TTCGAA 2 cut(s) 457, 2050
BstC8I GCNNGC 5 cut(s) 224, 648, 652, 1611, 2775
BstDEI CTNAG 7 cut(s) 270, 675, 1173, 1780, 2373, 2610, 2750
BstDSI CCRYGG 5 cut(s) 585, 1161, 1649, 2180, 2202
BstF5I GGATG 4 cut(s) 100, 610, 688, 2164
BstHHI GCGC 2 cut(s) 525, 716
BstKTI GATC 9 cut(s) 219, 981, 1290, 1627, 1707, 1870, 1942, 2722, 2758
BstMAI GTCTC 6 cut(s) 868, 1200, 1783, 1816, 1964, 2369
BstMBI GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
BstMCI CGRYCG 4 cut(s) 132, 1080, 1254, 2388
BstMWI GCNNNNNNNGC 9 cut(s) 23, 373, 522, 742, 1126, 1688, 2021, 2118, 2779
BstNI CCWGG 4 cut(s) 312, 466, 711, 2021
BstNSI RCATGY 3 cut(s) 1069, 1120, 1434
BstSCI CCNGG 4 cut(s) 310, 464, 709, 2019
BstSLI GKGCMC 2 cut(s) 648, 2017
BstV1I GCAGC 1 cut(s) 2757
BstV2I GAAGAC 5 cut(s) 117, 147, 180, 255, 2059
BstX2I RGATCY 2 cut(s) 978, 1704
BstXI CCANNNNNNTGG 3 cut(s) 1678, 1818, 2486
BstYI RGATCY 2 cut(s) 978, 1704
BstZI CGGCCG 2 cut(s) 129, 2385
Bsu15I ATCGAT 1 cut(s) 2718
BsuI GTATCC 1 cut(s) 2361
BsuTUI ATCGAT 1 cut(s) 2718
BtgI CCRYGG 5 cut(s) 585, 1161, 1649, 2180, 2202
BtgZI GCGATG 1 cut(s) 403
BtsCI GGATG 4 cut(s) 100, 610, 688, 2164
BtsI GCAGTG 1 cut(s) 1923
BtsIMutI CAGTG 4 cut(s) 1318, 1923, 2265, 2331
BveI ACCTGC 2 cut(s) 603, 2117
Cac8I GCNNGC 5 cut(s) 224, 648, 652, 1611, 2775
CciI TCATGA 2 cut(s) 1864, 1983
CfoI GCGC 2 cut(s) 525, 716
Cfr10I RCCGGY 2 cut(s) 127, 446
ClaI ATCGAT 1 cut(s) 2718
CseI GACGC 1 cut(s) 2552
CspCI CAANNNNNGTGG 4 cut(s) 222, 257, 567, 602
DdeI CTNAG 7 cut(s) 270, 675, 1173, 1780, 2373, 2610, 2750
DpnI GATC 9 cut(s) 218, 980, 1289, 1626, 1706, 1869, 1941, 2721, 2757
DpnII GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
DrdI GACNNNNNNGTC 1 cut(s) 1727
DseDI GACNNNNNNGTC 1 cut(s) 1727
EagI CGGCCG 2 cut(s) 129, 2385
Eam1104I CTCTTC 3 cut(s) 42, 757, 1761
EarI CTCTTC 3 cut(s) 42, 757, 1761
EclXI CGGCCG 2 cut(s) 129, 2385
Eco130I CCWWGG 5 cut(s) 768, 1161, 2180, 2202, 2693
Eco31I GGTCTC 2 cut(s) 868, 2369
Eco47I GGWCC 3 cut(s) 189, 1158, 1323
Eco52I CGGCCG 2 cut(s) 129, 2385
Eco57I CTGAAG 2 cut(s) 23, 1410
Eco88I CYCGRG 2 cut(s) 1693, 1725
EcoRI GAATTC 1 cut(s) 2096
EcoRII CCWGG 4 cut(s) 310, 464, 709, 2019
EcoT14I CCWWGG 5 cut(s) 768, 1161, 2180, 2202, 2693
EcoT22I ATGCAT 1 cut(s) 2424
ErhI CCWWGG 5 cut(s) 768, 1161, 2180, 2202, 2693
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 236, 268
FaqI GGGAC 4 cut(s) 202, 1590, 1668, 2674
FauNDI CATATG 1 cut(s) 527
Fnu4HI GCNGC 4 cut(s) 396, 612, 810, 2771
FokI GGATG 4 cut(s) 107, 617, 695, 2151
Fsp4HI GCNGC 4 cut(s) 396, 612, 810, 2771
FspAI RTGCGCAY 1 cut(s) 524
FspBI CTAG 5 cut(s) 107, 170, 926, 1344, 2729
FspI TGCGCA 1 cut(s) 524
GlaI GCGC 2 cut(s) 524, 715
GluI GCNGC 4 cut(s) 396, 612, 810, 2771
GsaI CCCAGC 2 cut(s) 1576, 1760
GsuI CTGGAG 1 cut(s) 1383
HapII CCGG 5 cut(s) 128, 447, 976, 2025, 2150
HgaI GACGC 1 cut(s) 2552
HhaI GCGC 2 cut(s) 525, 716
Hin1I GRCGYC 2 cut(s) 1803, 2157
Hin6I GCGC 2 cut(s) 523, 714
HinP1I GCGC 2 cut(s) 523, 714
HincII GTYRAC 1 cut(s) 2454
HindII GTYRAC 1 cut(s) 2454
HpaI GTTAAC 1 cut(s) 2454
HpaII CCGG 5 cut(s) 128, 447, 976, 2025, 2150
HphI GGTGA 2 cut(s) 976, 1235
Hpy166II GTNNAC 7 cut(s) 688, 1005, 1179, 1921, 2015, 2356, 2454
Hpy188I TCNGA 9 cut(s) 42, 207, 904, 1174, 1562, 1783, 1936, 2322, 2681
Hpy188III TCNNGA 9 cut(s) 161, 278, 1725, 1735, 1865, 1984, 2510, 2659, 2759
Hpy8I GTNNAC 7 cut(s) 688, 1005, 1179, 1921, 2015, 2356, 2454
Hpy99I CGWCG 3 cut(s) 779, 1805, 1808
HpyAV CCTTC 8 cut(s) 74, 238, 250, 1257, 1385, 1706, 1958, 2696
HpyCH4III ACNGT 2 cut(s) 12, 2326
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 373, 522, 742, 1126, 1688, 2021, 2118, 2779
HpyF3I CTNAG 7 cut(s) 270, 675, 1173, 1780, 2373, 2610, 2750
Hsp92I GRCGYC 2 cut(s) 1803, 2157
HspAI GCGC 2 cut(s) 523, 714
KpnI GGTACC 1 cut(s) 860
KspAI GTTAAC 1 cut(s) 2454
Kzo9I GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
LmnI GCTCC 9 cut(s) 83, 199, 231, 792, 1265, 1364, 1496, 1937, 2836
Lsp1109I GCAGC 1 cut(s) 2757
LweI GCATC 4 cut(s) 695, 908, 1553, 2100
MaeI CTAG 5 cut(s) 107, 170, 926, 1344, 2729
MaeIII GTNAC 4 cut(s) 296, 1068, 1698, 2305
MalI GATC 9 cut(s) 218, 980, 1289, 1626, 1706, 1869, 1941, 2721, 2757
MboI GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
MfeI CAATTG 2 cut(s) 358, 2169
MflI RGATCY 2 cut(s) 978, 1704
MhlI GDGCHC 6 cut(s) 88, 648, 797, 804, 1501, 2017
MlsI TGGCCA 3 cut(s) 557, 2071, 2185
MluNI TGGCCA 3 cut(s) 557, 2071, 2185
MlyI GAGTC 6 cut(s) 1117, 1715, 1737, 1785, 1818, 1870
MmeI TCCRAC 1 cut(s) 117
Mox20I TGGCCA 3 cut(s) 557, 2071, 2185
Mph1103I ATGCAT 1 cut(s) 2424
MscI TGGCCA 3 cut(s) 557, 2071, 2185
MseI TTAA 8 cut(s) 417, 440, 1539, 2045, 2175, 2453, 2604, 2802
MslI CAYNNNNRTG 3 cut(s) 730, 989, 1295
Msp20I TGGCCA 3 cut(s) 557, 2071, 2185
MspA1I CMGCKG 1 cut(s) 1361
MspCI CTTAAG 1 cut(s) 2801
MspI CCGG 5 cut(s) 128, 447, 976, 2025, 2150
MspR9I CCNGG 4 cut(s) 312, 466, 711, 2021
MunI CAATTG 2 cut(s) 358, 2169
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 4 cut(s) 312, 466, 711, 2021
MwoI GCNNNNNNNGC 9 cut(s) 23, 373, 522, 742, 1126, 1688, 2021, 2118, 2779
NcoI CCATGG 3 cut(s) 1161, 2180, 2202
NdeI CATATG 1 cut(s) 527
NdeII GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
NlaIV GGNNCC 7 cut(s) 190, 201, 858, 980, 1267, 1706, 2148
NmeAIII GCCGAG 7 cut(s) 157, 364, 424, 1105, 1294, 2437, 2647
NsbI TGCGCA 1 cut(s) 524
NsiI ATGCAT 1 cut(s) 2424
NspI RCATGY 3 cut(s) 1069, 1120, 1434
NspV TTCGAA 2 cut(s) 457, 2050
OliI CACNNNNGTG 1 cut(s) 989
PagI TCATGA 2 cut(s) 1864, 1983
PciI ACATGT 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 1599
PctI GAATGC 1 cut(s) 812
PfeI GAWTC 6 cut(s) 47, 1480, 1523, 2028, 2506, 2843
PflMI CCANNNNNTGG 4 cut(s) 869, 1222, 1492, 2823
PkrI GCNGC 4 cut(s) 397, 613, 811, 2772
PleI GAGTC 6 cut(s) 1117, 1715, 1736, 1784, 1818, 1870
PpsI GAGTC 6 cut(s) 1117, 1715, 1736, 1784, 1818, 1870
Ppu21I YACGTR 2 cut(s) 869, 2248
PscI ACATGT 1 cut(s) 1065
Psp1406I AACGTT 1 cut(s) 433
Psp6I CCWGG 4 cut(s) 310, 464, 709, 2019
PspFI CCCAGC 2 cut(s) 1572, 1756
PspGI CCWGG 4 cut(s) 310, 464, 709, 2019
PspN4I GGNNCC 7 cut(s) 190, 201, 858, 980, 1267, 1706, 2148
PsuI RGATCY 2 cut(s) 978, 1704
RseI CAYNNNNRTG 3 cut(s) 730, 989, 1295
SaqAI TTAA 8 cut(s) 417, 440, 1539, 2045, 2175, 2453, 2604, 2802
SatI GCNGC 4 cut(s) 396, 612, 810, 2771
Sau3AI GATC 9 cut(s) 216, 978, 1287, 1624, 1704, 1867, 1939, 2719, 2755
ScaI AGTACT 1 cut(s) 1348
SchI GAGTC 6 cut(s) 1117, 1715, 1737, 1785, 1818, 1870
ScrFI CCNGG 4 cut(s) 312, 466, 711, 2021
SduI GDGCHC 6 cut(s) 88, 648, 797, 804, 1501, 2017
SfaNI GCATC 4 cut(s) 695, 908, 1553, 2100
SfuI TTCGAA 2 cut(s) 457, 2050
SinI GGWCC 3 cut(s) 189, 1158, 1323
SmiMI CAYNNNNRTG 3 cut(s) 730, 989, 1295
SmlI CTYRAG 4 cut(s) 159, 970, 1854, 2801
SmoI CTYRAG 4 cut(s) 159, 970, 1854, 2801
SsiI CCGC 5 cut(s) 396, 611, 809, 1359, 1924
SspI AATATT 2 cut(s) 490, 2810
SspMI CTAG 5 cut(s) 107, 170, 926, 1344, 2729
StyD4I CCNGG 4 cut(s) 310, 464, 709, 2019
StyI CCWWGG 5 cut(s) 768, 1161, 2180, 2202, 2693
TaaI ACNGT 2 cut(s) 12, 2326
TaqII GACCGA 3 cut(s) 1066, 1115, 1268
TatI WGTACW 3 cut(s) 1113, 1346, 2353
TauI GCSGC 3 cut(s) 398, 614, 812
TfiI GAWTC 6 cut(s) 47, 1480, 1523, 2028, 2506, 2843
Tru1I TTAA 8 cut(s) 417, 440, 1539, 2045, 2175, 2453, 2604, 2802
Tru9I TTAA 8 cut(s) 417, 440, 1539, 2045, 2175, 2453, 2604, 2802
TscAI CASTG 4 cut(s) 1325, 1923, 2272, 2331
TseI GCWGC 1 cut(s) 2770
TspGWI ACGGA 2 cut(s) 1195, 1717
TspRI CASTG 4 cut(s) 1325, 1923, 2272, 2331
Van91I CCANNNNNTGG 4 cut(s) 869, 1222, 1492, 2823
Vha464I CTTAAG 1 cut(s) 2801
VneI GTGCAC 1 cut(s) 2013
VpaK11BI GGWCC 3 cut(s) 189, 1158, 1323
XapI RAATTY 9 cut(s) 58, 68, 181, 624, 663, 782, 1352, 2096, 2124
XceI RCATGY 3 cut(s) 1069, 1120, 1434
XcmI CCANNNNNNNNNTGG 3 cut(s) 61, 308, 2193
XspI CTAG 5 cut(s) 107, 170, 926, 1344, 2729
ZraI GACGTC 2 cut(s) 1804, 2158
ZrmI AGTACT 1 cut(s) 1348
Zsp2I ATGCAT 1 cut(s) 2424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.