Rw1G006680

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000160
Physical Location & Seq
Forward (+)
13719536 .. 13722658
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G006680.1

Sequence Viewer

Length: 2358 bp
ATGGTGGAGAAAGAGGCTTATGATCGCATCGCGACCTTAGAGGTCGTTAATGAAGAACTTTCTGACCCGGAGGGCGAAGATGAAGTCAGCATTCAGCTTGCCCCAGCAGCGCTGGACGATACGCCTCCTAAAGTTAGAGACCCTACTGAGAAGGTTAATCTGGGAACAGTGAGCGAGCCTATGGAGGTGGCTATCAGTGCTTATTTAGAGCCTAGCGAAAAACAGAGGCTCACTGAATTGCTATTGGAATTTAAGGATTGCTTTGCGGAGAAGTATGAAGACATGCCCGGCCTGTCACCAGATTTGGTTTGTCATCAACTACCAACTCTTCCTGACAAGAGGCCCGTCAAGCAAGAACCTCGACGAATGAATTCGGAGACCCAGATTCTCGTAAAGGAGGAGGTAGAGAAAATGCACAAGTCGGGCATTATCAGGGTCGCCAAGTATAATCAGTGGCTATCGAATATAGTACATGTCCGGAAGAAGAACGGCAAGATGAGAGTCTGCGTTGATTATAGAGACTTAAATCTTGCTACGCCTAAGGATGTTTACCCCATGCCGGTCGCGGATATGTTGGTAGATGCTGATATCACCATATTCCGGTCGCTGAAGAAGACAGACACTAGACCGCTTTCCGCTGCCCTGGAGTGCAAGTTGCGGCATTACATGTTATCCTTTACCACCTGCATCATCGCCCAAACCGATTTGGTCAAATACATGCTGTCACGGCCTATCTTGAGAGGTCGCATTGGCAAATGGGTATTGGCTCTCTCAGAGTTTTCGCTCCAATATGTACCTCAGAAAGCTGTCAAGGGGCAGGCCATCGCAGACTTTCTCGCACATCATCCTACATTAGAGATACCCACACTGAAGGAGTTAGAGATAGCGTCCACCACTACGACTCGACCAGATTTGGCGCGCATCCCTGAGTACGCAATTTGGTACCACGCCACAGTCTCCCTGCAGCCTTGGGTTTTGTTCTTTGATGGCTTAAGAACTGACACACTGGCTGGTGCAGGGATTGTTTTGGAAAACCCAACCGGTGACCGTTTTTCTTATTCCTTCCAACTAGAGTTCCAATGTACTAATAATCAGGCGGAATATGAAGCTCTTATTATTGGACTCGAGGTCTTATTGGAAATGGGCGTCAAGGACGTTCAGATTCGCGGCGACTCTCAGCTCGTTATAAACCAGCTCCAAGAAAAGTATCGATGTGCGAGTTGGTTGCTCGTACCATACCTGAATCGCGCCATCGAGCCTCTGGACCAATTTGACGACGTCGATCTGGAGCATATACCACGCGAGCGTAACTTTGCAGCCAATGAGCTTGCTCAGCTGGCCACAGGCATCACATTGAAGTATGGAGTGCGCGAGCGCATTTTGAAAGTTGAGCGTCGCACATTACCTTCATGGCTTGCACGACCTGATCCACCAGATGATCCCGTGGTCGCGGTGCTAGAACCGATTGATGTGGATTGGCGAATCCCTCTGATCGAATATCTCAAACAACCAGACCCCAGAGCTGACAGGAAGATTCGCTTCCTCGCGCTAAACTACTTCCTTAGAGGTGATGAGTTGCTGCGCCGTGGGGAGGATGGTATGGACTTTCGGTGCGTCTATGGCCGAGAAGCTAAACGATTAATGCGCGAAGTACACACGGGGATATGCGGGGCTCATCAAGCGGGACCTAAGATGCGTTGGTTTCTGCGACGCCATGTCTATTATTGGCCCAACATTTTAAAGGATTGTATCGCATTTGCCAAAGGCTGTGAAGATTGTCAGGCGCACGGTCCAGTCCAACATGTTCCTTCCATTCCTATCCAGCCTATTATTAAGCCTTGGCCTGCGCGAGGTTGGGCGTTGGACTTGATTGGGATGATCCATCCCCATTCTTCCCTTCAGCACAAGTTTATCATCGTCGCTACTGACTACTTCACCAAATGGGTTGAAGCGGAGCCTTTGAAAGAGGCCTCCGGTGCCACCATTCGCCAGTTTATTTTCCGTAACATTCTCTGCAGGTTTGGTATCCCCGAGGTGCTGGTTTCGGACAGGGGGGCAGCGTTCATGGGAGGCCCGGTCGAGGAACTAGTCAATGATTTTGGCATCCAATTCGTCCACAGCACTCCGTATTACGCTCAATCTAATGGTCAAGCAGAGGCTAGTAACAAGACAATTATTACCTTACTGAAGAAGATGTTGGTAGAGAACCCTCGACAATGGCATGATACTTTGTATGAGACACTTTGGGCCTATCGCACTTCTAAACGGAATCCCACCGCAACAACGCCGTACGCCCTCATGTTCGGTCATGACGCTGTCTTACCGTTAGAAATTAACGTTCATTCTCTGCGCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

785

Amino Acids

89.76

Weight (kDa)

6.11

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 328 - 446 7.2e-20 Reverse transcriptase-like
Integrase_H2C2 PF17921 543 - 596 5.5e-08 Integrase zinc binding domain
rve PF00665 617 - 711 5.2e-08 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1187
AarI CACCTGC 1 cut(s) 692
AatII GACGTC 1 cut(s) 1281
Acc36I ACCTGC 2 cut(s) 692, 2007
Acc65I GGTACC 1 cut(s) 942
AccB1I GGYRCC 2 cut(s) 942, 1976
AccIII TCCGGA 1 cut(s) 477
AclI AACGTT 1 cut(s) 2337
AclWI GGATC 3 cut(s) 1421, 1433, 1873
AcoI YGGCCR 2 cut(s) 1338, 1621
AcsI RAATTY 2 cut(s) 248, 370
AcuI CTGAAG 4 cut(s) 629, 890, 1883, 2207
AcyI GRCGYC 3 cut(s) 1146, 1278, 1711
AfaI GTAC 8 cut(s) 471, 795, 932, 944, 1084, 1233, 1653, 2291
AfeI AGCGCT 1 cut(s) 111
AfiI CCNNNNNNNGG 5 cut(s) 559, 600, 1591, 1850, 2080
AflII CTTAAG 1 cut(s) 991
AflIII ACRYGT 3 cut(s) 472, 666, 1801
AgeI ACCGGT 1 cut(s) 1040
AgsI TTSAA 4 cut(s) 1357, 1384, 1949, 1963
AhdI GACNNNNNGTC 2 cut(s) 1127, 1715
AhlI ACTAGT 1 cut(s) 2086
AjnI CCWGG 1 cut(s) 642
AjuI GAANNNNNNNTTGG 2 cut(s) 2180, 2212
AluBI AGCT 9 cut(s) 97, 806, 1109, 1180, 1195, 1327, 1336, 1523, 1631
AluI AGCT 9 cut(s) 97, 806, 1109, 1180, 1195, 1327, 1336, 1523, 1631
Alw26I GTCTC 5 cut(s) 132, 371, 513, 961, 2231
AlwI GGATC 3 cut(s) 1421, 1433, 1873
Ama87I CYCGRG 2 cut(s) 1124, 2030
Aor13HI TCCGGA 1 cut(s) 477
Aor51HI AGCGCT 1 cut(s) 111
ApeKI GCWGC 6 cut(s) 107, 638, 964, 1316, 1579, 2057
ApoI RAATTY 2 cut(s) 248, 370
ArsI GACNNNNNNTTYG 2 cut(s) 69, 101
AseI ATTAAT 1 cut(s) 1640
AsiGI ACCGGT 1 cut(s) 1040
Asp700I GAANNNNTTC 1 cut(s) 370
Asp718I GGTACC 1 cut(s) 942
AspS9I GGNCC 7 cut(s) 342, 1264, 1685, 1728, 1790, 2072, 2247
AsuC2I CCSGG 3 cut(s) 68, 288, 2075
AsuHPI GGTGA 5 cut(s) 288, 583, 1055, 1580, 1927
AvaI CYCGRG 2 cut(s) 1124, 2030
AvaII GGWCC 3 cut(s) 1264, 1685, 1790
AxyI CCTNAGG 1 cut(s) 540
BalI TGGCCA 1 cut(s) 1340
BanI GGYRCC 2 cut(s) 942, 1976
BanII GRGCYC 1 cut(s) 1675
BbsI GAAGAC 2 cut(s) 285, 620
BbvI GCAGC 6 cut(s) 119, 625, 976, 1328, 1566, 2069
BccI CCATC 5 cut(s) 830, 980, 1259, 1589, 1890
BceAI ACGGC 4 cut(s) 505, 743, 1569, 2272
BcgI CGANNNNNNTGC 6 cut(s) 341, 375, 1207, 1241, 2092, 2126
BciT130I CCWGG 1 cut(s) 644
BciVI GTATCC 1 cut(s) 2036
BcnI CCSGG 3 cut(s) 68, 288, 2075
BcoDI GTCTC 5 cut(s) 132, 371, 513, 961, 2231
BcuI ACTAGT 1 cut(s) 2086
BfaI CTAG 6 cut(s) 213, 624, 1070, 1457, 2087, 2160
BfmI CTRYAG 2 cut(s) 962, 2014
BfoI RGCGCY 1 cut(s) 113
BfrI CTTAAG 1 cut(s) 991
BfuAI ACCTGC 2 cut(s) 692, 2007
BfuI GTATCC 1 cut(s) 2036
BisI GCNGC 8 cut(s) 108, 639, 659, 965, 1168, 1317, 1580, 2058
BlpI GCTNAGC 1 cut(s) 1332
BlsI GCNGC 8 cut(s) 109, 640, 660, 966, 1169, 1318, 1581, 2059
Bme1390I CCNGG 4 cut(s) 68, 288, 644, 2075
Bme18I GGWCC 3 cut(s) 1264, 1685, 1790
BmeRI GACNNNNNGTC 2 cut(s) 1127, 1715
BmeT110I CYCGRG 2 cut(s) 1124, 2030
BmgT120I GGNCC 7 cut(s) 342, 1264, 1685, 1728, 1790, 2072, 2247
BmiI GGNNCC 4 cut(s) 944, 1686, 1956, 1978
BmrFI CCNGG 4 cut(s) 68, 288, 644, 2075
BmsI GCATC 7 cut(s) 36, 571, 696, 930, 1356, 1683, 2112
BpiI GAAGAC 2 cut(s) 285, 620
BpmI CTGGAG 2 cut(s) 665, 1307
Bpu1102I GCTNAGC 1 cut(s) 1332
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 3 cut(s) 68, 288, 2075
Bsa29I ATCGAT 1 cut(s) 1210
BsaHI GRCGYC 3 cut(s) 1146, 1278, 1711
BsaI GGTCTC 2 cut(s) 132, 371
BsaJI CCNNGG 6 cut(s) 642, 968, 1443, 1585, 1838, 2031
BsaWI WCCGGW 4 cut(s) 477, 600, 1040, 1973
BsaXI ACNNNNNCTCC 4 cut(s) 638, 668, 2061, 2091
Bsc4I CCNNNNNNNGG 5 cut(s) 559, 600, 1591, 1850, 2080
Bse118I RCCGGY 2 cut(s) 559, 1040
Bse1I ACTGG 3 cut(s) 1011, 1793, 1990
Bse21I CCTNAGG 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 477
BseBI CCWGG 1 cut(s) 644
BseCI ATCGAT 1 cut(s) 1210
BseDI CCNNGG 6 cut(s) 642, 968, 1443, 1585, 1838, 2031
BseGI GGATG 7 cut(s) 550, 844, 921, 1600, 1881, 1882, 2103
BseLI CCNNNNNNNGG 5 cut(s) 559, 600, 1591, 1850, 2080
BseMII CTCAG 6 cut(s) 138, 786, 812, 918, 1190, 1346
BseNI ACTGG 3 cut(s) 1011, 1793, 1990
BsePI GCGCGC 1 cut(s) 917
BseRI GAGGAG 1 cut(s) 413
BseXI GCAGC 6 cut(s) 119, 625, 976, 1328, 1566, 2069
BseYI CCCAGC 1 cut(s) 103
BsgI GTGCAG 1 cut(s) 1035
Bsh1285I CGRYCG 3 cut(s) 564, 605, 2079
BshNI GGYRCC 2 cut(s) 942, 1976
BshTI ACCGGT 1 cut(s) 1040
BshVI ATCGAT 1 cut(s) 1210
BsiEI CGRYCG 3 cut(s) 564, 605, 2079
BsiHKCI CYCGRG 2 cut(s) 1124, 2030
BsiSI CCGG 8 cut(s) 68, 288, 478, 560, 601, 1041, 1974, 2075
BsiWI CGTACG 1 cut(s) 2289
BslFI GGGAC 1 cut(s) 1698
BslI CCNNNNNNNGG 5 cut(s) 559, 600, 1591, 1850, 2080
BsmAI GTCTC 5 cut(s) 132, 371, 513, 961, 2231
BsmFI GGGAC 1 cut(s) 1698
BsmI GAATGC 1 cut(s) 90
Bso31I GGTCTC 2 cut(s) 132, 371
BsoBI CYCGRG 2 cut(s) 1124, 2030
Bsp1286I GDGCHC 1 cut(s) 1675
Bsp13I TCCGGA 1 cut(s) 477
Bsp143I GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
Bsp1720I GCTNAGC 1 cut(s) 1332
Bsp68I TCGCGA 1 cut(s) 32
BspCNI CTCAG 6 cut(s) 139, 785, 811, 919, 1189, 1345
BspDI ATCGAT 1 cut(s) 1210
BspEI TCCGGA 1 cut(s) 477
BspHI TCATGA 1 cut(s) 2308
BspLI GGNNCC 4 cut(s) 944, 1686, 1956, 1978
BspMAI CTGCAG 2 cut(s) 966, 2018
BspMI ACCTGC 2 cut(s) 692, 2007
BspPI GGATC 3 cut(s) 1421, 1433, 1873
BspT107I GGYRCC 2 cut(s) 942, 1976
BspTI CTTAAG 1 cut(s) 991
BspTNI GGTCTC 2 cut(s) 132, 371
BsrFI RCCGGY 2 cut(s) 559, 1040
BsrI ACTGG 3 cut(s) 1011, 1793, 1990
BssAI RCCGGY 2 cut(s) 559, 1040
BssECI CCNNGG 6 cut(s) 642, 968, 1443, 1585, 1838, 2031
BssHII GCGCGC 1 cut(s) 917
BssMI GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
BssNI GRCGYC 3 cut(s) 1146, 1278, 1711
BssT1I CCWWGG 2 cut(s) 968, 1838
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 5 cut(s) 169, 955, 1049, 1790, 2325
Bst6I CTCTTC 1 cut(s) 333
BstACI GRCGYC 3 cut(s) 1146, 1278, 1711
BstAFI CTTAAG 1 cut(s) 991
BstDSI CCRYGG 2 cut(s) 1443, 1585
BstEII GGTNACC 1 cut(s) 1043
BstENI CCTNNNNNAGG 1 cut(s) 1848
BstF5I GGATG 7 cut(s) 550, 844, 921, 1600, 1881, 1882, 2103
BstH2I RGCGCY 1 cut(s) 113
BstKTI GATC 6 cut(s) 25, 1285, 1429, 1441, 1494, 1881
BstMAI GTCTC 5 cut(s) 132, 371, 513, 961, 2231
BstMBI GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
BstMCI CGRYCG 3 cut(s) 564, 605, 2079
BstMWI GCNNNNNNNGC 9 cut(s) 107, 197, 349, 727, 1333, 1337, 1620, 1679, 1976
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 5 cut(s) 286, 476, 670, 721, 1805
BstPI GGTNACC 1 cut(s) 1043
BstSCI CCNGG 4 cut(s) 66, 286, 642, 2073
BstSFI CTRYAG 2 cut(s) 962, 2014
BstV1I GCAGC 6 cut(s) 119, 625, 976, 1328, 1566, 2069
BstV2I GAAGAC 2 cut(s) 285, 620
Bsu15I ATCGAT 1 cut(s) 1210
Bsu36I CCTNAGG 1 cut(s) 540
BsuI GTATCC 1 cut(s) 2036
BsuTUI ATCGAT 1 cut(s) 1210
BtgI CCRYGG 2 cut(s) 1443, 1585
BtgZI GCGATG 3 cut(s) 13, 676, 808
BtsCI GGATG 7 cut(s) 550, 844, 921, 1600, 1881, 1882, 2103
BtsIMutI CAGTG 6 cut(s) 174, 202, 231, 458, 866, 1004
BtuMI TCGCGA 1 cut(s) 32
BveI ACCTGC 2 cut(s) 692, 2007
CciI TCATGA 1 cut(s) 2308
Cfr10I RCCGGY 2 cut(s) 559, 1040
Cfr13I GGNCC 7 cut(s) 342, 1264, 1685, 1728, 1790, 2072, 2247
ClaI ATCGAT 1 cut(s) 1210
CseI GACGC 6 cut(s) 876, 1135, 1382, 1603, 1719, 2321
Csp6I GTAC 8 cut(s) 470, 794, 931, 943, 1083, 1232, 1652, 2290
CspAI ACCGGT 1 cut(s) 1040
CviQI GTAC 8 cut(s) 470, 794, 931, 943, 1083, 1232, 1652, 2290
DpnI GATC 6 cut(s) 24, 1284, 1428, 1440, 1493, 1880
DpnII GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
DraI TTTAAA 1 cut(s) 1740
DriI GACNNNNNGTC 2 cut(s) 1127, 1715
EaeI YGGCCR 2 cut(s) 1338, 1621
Eam1104I CTCTTC 1 cut(s) 333
Eam1105I GACNNNNNGTC 2 cut(s) 1127, 1715
EarI CTCTTC 1 cut(s) 333
EciI GGCGGA 1 cut(s) 1112
Eco130I CCWWGG 2 cut(s) 968, 1838
Eco147I AGGCCT 1 cut(s) 1970
Eco24I GRGCYC 1 cut(s) 1675
Eco31I GGTCTC 2 cut(s) 132, 371
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 3 cut(s) 1264, 1685, 1790
Eco47III AGCGCT 1 cut(s) 111
Eco57I CTGAAG 4 cut(s) 629, 890, 1883, 2207
Eco81I CCTNAGG 1 cut(s) 540
Eco88I CYCGRG 2 cut(s) 1124, 2030
Eco91I GGTNACC 1 cut(s) 1043
EcoNI CCTNNNNNAGG 1 cut(s) 1848
EcoO109I RGGNCCY 1 cut(s) 1685
EcoO65I GGTNACC 1 cut(s) 1043
EcoRI GAATTC 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 642
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 2 cut(s) 968, 1838
EcoT38I GRGCYC 1 cut(s) 1675
ErhI CCWWGG 2 cut(s) 968, 1838
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 1523, 1555
FaqI GGGAC 1 cut(s) 1698
FauI CCCGC 2 cut(s) 1661, 1675
Fnu4HI GCNGC 8 cut(s) 108, 639, 659, 965, 1168, 1317, 1580, 2058
FokI GGATG 7 cut(s) 557, 831, 908, 1607, 1869, 1888, 2090
FriOI GRGCYC 1 cut(s) 1675
Fsp4HI GCNGC 8 cut(s) 108, 639, 659, 965, 1168, 1317, 1580, 2058
FspBI CTAG 6 cut(s) 213, 624, 1070, 1457, 2087, 2160
GluI GCNGC 8 cut(s) 108, 639, 659, 965, 1168, 1317, 1580, 2058
GsaI CCCAGC 1 cut(s) 107
GsuI CTGGAG 2 cut(s) 665, 1307
HaeII RGCGCY 1 cut(s) 113
HapII CCGG 8 cut(s) 68, 288, 478, 560, 601, 1041, 1974, 2075
HgaI GACGC 6 cut(s) 876, 1135, 1382, 1603, 1719, 2321
Hin1I GRCGYC 3 cut(s) 1146, 1278, 1711
HpaII CCGG 8 cut(s) 68, 288, 478, 560, 601, 1041, 1974, 2075
HphI GGTGA 5 cut(s) 288, 583, 1055, 1580, 1927
Hpy166II GTNNAC 4 cut(s) 550, 891, 1654, 2116
Hpy188I TCNGA 7 cut(s) 64, 376, 775, 801, 1161, 1491, 2047
Hpy188III TCNNGA 7 cut(s) 31, 332, 478, 736, 1262, 1286, 2309
Hpy8I GTNNAC 4 cut(s) 550, 891, 1654, 2116
Hpy99I CGWCG 6 cut(s) 366, 1280, 1283, 1398, 1713, 1922
HpyAV CCTTC 6 cut(s) 145, 865, 1072, 1416, 1818, 1907
HpyCH4III ACNGT 5 cut(s) 169, 955, 1049, 1790, 2325
HpyCH4IV ACGT 3 cut(s) 1155, 1278, 2337
HpyCH4V TGCA 8 cut(s) 415, 651, 687, 964, 1016, 1316, 1418, 2016
HpyF10VI GCNNNNNNNGC 9 cut(s) 107, 197, 349, 727, 1333, 1337, 1620, 1679, 1976
HpySE526I ACGT 3 cut(s) 1155, 1278, 2337
Hsp92I GRCGYC 3 cut(s) 1146, 1278, 1711
Kpn2I TCCGGA 1 cut(s) 477
KpnI GGTACC 1 cut(s) 946
Kzo9I GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
LmnI GCTCC 4 cut(s) 789, 1200, 1288, 1954
Lsp1109I GCAGC 6 cut(s) 119, 625, 976, 1328, 1566, 2069
LweI GCATC 7 cut(s) 36, 571, 696, 930, 1356, 1683, 2112
MaeI CTAG 6 cut(s) 213, 624, 1070, 1457, 2087, 2160
MaeII ACGT 3 cut(s) 1155, 1278, 2337
MaeIII GTNAC 6 cut(s) 294, 723, 1043, 1307, 2003, 2162
MalI GATC 6 cut(s) 24, 1284, 1428, 1440, 1493, 1880
MboI GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
MhlI GDGCHC 1 cut(s) 1675
MlsI TGGCCA 1 cut(s) 1340
MluCI AATT 8 cut(s) 236, 248, 370, 936, 1268, 2108, 2172, 2331
MluNI TGGCCA 1 cut(s) 1340
MlyI GAGTC 4 cut(s) 510, 895, 1116, 1166
MmeI TCCRAC 3 cut(s) 1090, 1822, 1842
Mox20I TGGCCA 1 cut(s) 1340
MroI TCCGGA 1 cut(s) 477
MroXI GAANNNNTTC 1 cut(s) 370
MscI TGGCCA 1 cut(s) 1340
MseI TTAA 9 cut(s) 48, 156, 252, 524, 992, 1640, 1739, 1833, 2334
Msp20I TGGCCA 1 cut(s) 1340
MspA1I CMGCKG 2 cut(s) 638, 1336
MspCI CTTAAG 1 cut(s) 991
MspI CCGG 8 cut(s) 68, 288, 478, 560, 601, 1041, 1974, 2075
MspR9I CCNGG 4 cut(s) 68, 288, 644, 2075
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 1 cut(s) 644
MwoI GCNNNNNNNGC 9 cut(s) 107, 197, 349, 727, 1333, 1337, 1620, 1679, 1976
NciI CCSGG 3 cut(s) 68, 288, 2075
NdeII GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
NlaIV GGNNCC 4 cut(s) 944, 1686, 1956, 1978
NmeAIII GCCGAG 1 cut(s) 1649
NmuCI GTSAC 3 cut(s) 294, 723, 1043
NruI TCGCGA 1 cut(s) 32
NspI RCATGY 5 cut(s) 286, 476, 670, 721, 1805
PaeR7I CTCGAG 1 cut(s) 1124
PagI TCATGA 1 cut(s) 2308
PaqCI CACCTGC 1 cut(s) 692
PauI GCGCGC 1 cut(s) 917
PceI AGGCCT 1 cut(s) 1970
PciI ACATGT 3 cut(s) 472, 666, 1801
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PctI GAATGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 370
PfeI GAWTC 6 cut(s) 385, 1162, 1243, 1482, 1534, 2269
Pfl23II CGTACG 1 cut(s) 2289
PflFI GACNNNGTC 2 cut(s) 1277, 2315
PinAI ACCGGT 1 cut(s) 1040
PkrI GCNGC 8 cut(s) 109, 640, 660, 966, 1169, 1318, 1581, 2059
PleI GAGTC 4 cut(s) 509, 895, 1116, 1166
PpsI GAGTC 4 cut(s) 509, 895, 1116, 1166
PpuMI RGGWCCY 1 cut(s) 1685
PscI ACATGT 3 cut(s) 472, 666, 1801
PshBI ATTAAT 1 cut(s) 1640
PsiI TTATAA 1 cut(s) 1187
Psp1406I AACGTT 1 cut(s) 2337
Psp5II RGGWCCY 1 cut(s) 1685
Psp6I CCWGG 1 cut(s) 642
PspEI GGTNACC 1 cut(s) 1043
PspFI CCCAGC 1 cut(s) 103
PspGI CCWGG 1 cut(s) 642
PspLI CGTACG 1 cut(s) 2289
PspN4I GGNNCC 4 cut(s) 944, 1686, 1956, 1978
PspPI GGNCC 7 cut(s) 342, 1264, 1685, 1728, 1790, 2072, 2247
PspPPI RGGWCCY 1 cut(s) 1685
PspXI VCTCGAGB 1 cut(s) 1124
PstI CTGCAG 2 cut(s) 966, 2018
PsyI GACNNNGTC 2 cut(s) 1277, 2315
PteI GCGCGC 1 cut(s) 917
PvuII CAGCTG 1 cut(s) 1336
RruI TCGCGA 1 cut(s) 32
RsaI GTAC 8 cut(s) 471, 795, 932, 944, 1084, 1233, 1653, 2291
RsaNI GTAC 8 cut(s) 470, 794, 931, 943, 1083, 1232, 1652, 2290
SaqAI TTAA 9 cut(s) 48, 156, 252, 524, 992, 1640, 1739, 1833, 2334
SatI GCNGC 8 cut(s) 108, 639, 659, 965, 1168, 1317, 1580, 2058
Sau3AI GATC 6 cut(s) 22, 1282, 1426, 1438, 1491, 1878
Sau96I GGNCC 7 cut(s) 342, 1264, 1685, 1728, 1790, 2072, 2247
SchI GAGTC 4 cut(s) 510, 895, 1116, 1166
ScrFI CCNGG 4 cut(s) 68, 288, 644, 2075
SduI GDGCHC 1 cut(s) 1675
SfaNI GCATC 7 cut(s) 36, 571, 696, 930, 1356, 1683, 2112
SfcI CTRYAG 2 cut(s) 962, 2014
Sfr274I CTCGAG 1 cut(s) 1124
SinI GGWCC 3 cut(s) 1264, 1685, 1790
SlaI CTCGAG 1 cut(s) 1124
SmlI CTYRAG 3 cut(s) 736, 991, 1124
SmoI CTYRAG 3 cut(s) 736, 991, 1124
SpeI ACTAGT 1 cut(s) 2086
Sse9I AATT 8 cut(s) 236, 248, 370, 936, 1268, 2108, 2172, 2331
SseBI AGGCCT 1 cut(s) 1970
SspMI CTAG 6 cut(s) 213, 624, 1070, 1457, 2087, 2160
StuI AGGCCT 1 cut(s) 1970
StyD4I CCNGG 4 cut(s) 66, 286, 642, 2073
StyI CCWWGG 2 cut(s) 968, 1838
TaaI ACNGT 5 cut(s) 169, 955, 1049, 1790, 2325
TaiI ACGT 3 cut(s) 1158, 1281, 2340
TaqII GACCGA 1 cut(s) 2294
TasI AATT 8 cut(s) 236, 248, 370, 936, 1268, 2108, 2172, 2331
TatI WGTACW 3 cut(s) 469, 1082, 1651
TauI GCSGC 2 cut(s) 661, 1170
TfiI GAWTC 6 cut(s) 385, 1162, 1243, 1482, 1534, 2269
Tru1I TTAA 9 cut(s) 48, 156, 252, 524, 992, 1640, 1739, 1833, 2334
Tru9I TTAA 9 cut(s) 48, 156, 252, 524, 992, 1640, 1739, 1833, 2334
TscAI CASTG 6 cut(s) 174, 202, 238, 458, 873, 1011
TseFI GTSAC 3 cut(s) 294, 723, 1043
TseI GCWGC 6 cut(s) 107, 638, 964, 1316, 1579, 2057
Tsp45I GTSAC 3 cut(s) 294, 723, 1043
TspDTI ATGAA 8 cut(s) 66, 96, 291, 383, 1119, 1398, 2053, 2330
TspGWI ACGGA 3 cut(s) 1991, 2115, 2281
TspRI CASTG 6 cut(s) 174, 202, 238, 458, 873, 1011
Tth111I GACNNNGTC 2 cut(s) 1277, 2315
Vha464I CTTAAG 1 cut(s) 991
VpaK11BI GGWCC 3 cut(s) 1264, 1685, 1790
VspI ATTAAT 1 cut(s) 1640
XagI CCTNNNNNAGG 1 cut(s) 1848
XapI RAATTY 2 cut(s) 248, 370
XceI RCATGY 5 cut(s) 286, 476, 670, 721, 1805
XcmI CCANNNNNNNNNTGG 1 cut(s) 1258
XhoI CTCGAG 1 cut(s) 1124
XmnI GAANNNNTTC 1 cut(s) 370
XspI CTAG 6 cut(s) 213, 624, 1070, 1457, 2087, 2160
ZraI GACGTC 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.