pycom13g22710

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000160
Physical Location & Seq
Reverse (-)
20460480 .. 20460968
489 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g22710.1

Sequence Viewer

Length: 489 bp
ATGGAAATCACAACTCTTGAGGTATATGACGACTCCAAGCTCATAATTAATCAACTCTTAATTGAATATGAGGTGAGGAAAGATGATCTTGTCCCATACTTCCGGCTGGCAACTCAACTGCTACAAAAGTTCAAGGCAGTGACACTAGAACATGTGCCAAGAAAGGAAAATCAAATGGCAGACGCTCTCGCCAATCTAGCCTCGAGTATGACACTAGGAAAAGATGAAGTTGTAGACGTGCCAGTTTGCCAAACATGGGTGATCCCGCTCGTTAATGAAATGTTACTGAATGATACAAATGTCATCTCAGTACTTCCAGTCGATGCTGAAGAGTGGAGACAGTCGCTGATCGACTACTTAGAGCACGGAAAGCTTCCAGATGATCTTAGAAAAAGGAAAAGAGATGGTGGCGACAGAATTATTGAAAAGAAGAAAATAGGGGAAGGATGTTGCACGACACCACCTTGCAAACTTCCGATCTGGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

163

Amino Acids

18.58

Weight (kDa)

5.01

Isoelectric Point (pI)

30.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 3 - 67 5.1e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 268
AccI GTMKAC 1 cut(s) 234
AciI CCGC 1 cut(s) 266
AclWI GGATC 1 cut(s) 256
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 3 cut(s) 65, 133, 425
AjiI CACGTC 1 cut(s) 238
AluBI AGCT 2 cut(s) 40, 373
AluI AGCT 2 cut(s) 40, 373
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 1 cut(s) 331
AlwI GGATC 1 cut(s) 256
AlwNI CAGNNNCTG 1 cut(s) 346
Ama87I CYCGRG 1 cut(s) 202
AseI ATTAAT 1 cut(s) 48
AsuHPI GGTGA 2 cut(s) 85, 271
AvaI CYCGRG 1 cut(s) 202
BaeI ACNNNNGTAYC 2 cut(s) 285, 318
Bbv12I GWGCWC 1 cut(s) 366
BccI CCATC 1 cut(s) 398
BcoDI GTCTC 1 cut(s) 331
BfaI CTAG 3 cut(s) 146, 197, 215
BmcAI AGTACT 1 cut(s) 312
BmeT110I CYCGRG 1 cut(s) 202
BmgBI CACGTC 1 cut(s) 238
BmsI GCATC 1 cut(s) 313
BpuEI CTTGAG 1 cut(s) 38
Bsc4I CCNNNNNNNGG 1 cut(s) 256
Bse1I ACTGG 2 cut(s) 242, 317
BseGI GGATG 1 cut(s) 452
BseLI CCNNNNNNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 2 cut(s) 242, 317
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 103
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 5 cut(s) 85, 261, 348, 382, 477
BspACI CCGC 1 cut(s) 266
BspCNI CTCAG 1 cut(s) 320
BspPI GGATC 1 cut(s) 256
BsrBI CCGCTC 1 cut(s) 268
BsrI ACTGG 2 cut(s) 242, 317
BssMI GATC 5 cut(s) 85, 261, 348, 382, 477
Bst4CI ACNGT 1 cut(s) 342
Bst6I CTCTTC 1 cut(s) 324
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 3 cut(s) 307, 358, 386
BstF5I GGATG 1 cut(s) 452
BstKTI GATC 5 cut(s) 88, 264, 351, 385, 480
BstMAI GTCTC 1 cut(s) 331
BstMBI GATC 5 cut(s) 85, 261, 348, 382, 477
BstMWI GCNNNNNNNGC 2 cut(s) 197, 370
BstNSI RCATGY 1 cut(s) 155
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 1 cut(s) 452
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 108
CaiI CAGNNNCTG 1 cut(s) 346
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 311
CviAII CATG 2 cut(s) 152, 255
CviJI RGCY 4 cut(s) 40, 106, 200, 373
CviKI_1 RGCY 4 cut(s) 40, 106, 200, 373
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 3 cut(s) 307, 358, 386
DpnI GATC 5 cut(s) 87, 263, 350, 384, 479
DpnII GATC 5 cut(s) 85, 261, 348, 382, 477
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 202
FaeI CATG 2 cut(s) 155, 258
FaiI YATR 8 cut(s) 25, 27, 44, 69, 97, 153, 209, 256
FalI AAGNNNNNCTT 2 cut(s) 72, 104
FaqI GGGAC 1 cut(s) 77
FatI CATG 2 cut(s) 151, 254
FauI CCCGC 1 cut(s) 273
FblI GTMKAC 1 cut(s) 234
FokI GGATG 1 cut(s) 459
FspBI CTAG 3 cut(s) 146, 197, 215
HapII CCGG 1 cut(s) 103
HgaI GACGC 1 cut(s) 191
Hin1II CATG 2 cut(s) 155, 258
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 1 cut(s) 32
HpaII CCGG 1 cut(s) 103
HphI GGTGA 2 cut(s) 85, 271
Hpy166II GTNNAC 1 cut(s) 235
Hpy188I TCNGA 1 cut(s) 477
Hpy188III TCNNGA 3 cut(s) 17, 377, 481
Hpy8I GTNNAC 1 cut(s) 235
HpyAV CCTTC 1 cut(s) 437
HpyCH4III ACNGT 1 cut(s) 342
HpyCH4IV ACGT 1 cut(s) 237
HpyCH4V TGCA 2 cut(s) 453, 468
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 370
HpyF3I CTNAG 3 cut(s) 307, 358, 386
HpySE526I ACGT 1 cut(s) 237
Hsp92II CATG 2 cut(s) 155, 258
Kzo9I GATC 5 cut(s) 85, 261, 348, 382, 477
LpnPI CCDG 6 cut(s) 92, 116, 255, 330, 390, 466
LweI GCATC 1 cut(s) 313
MaeI CTAG 3 cut(s) 146, 197, 215
MaeII ACGT 1 cut(s) 237
MaeIII GTNAC 2 cut(s) 139, 282
MalI GATC 5 cut(s) 87, 263, 350, 384, 479
MbiI CCGCTC 1 cut(s) 268
MboI GATC 5 cut(s) 85, 261, 348, 382, 477
MboII GAAGA 2 cut(s) 341, 442
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 3 cut(s) 45, 60, 417
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 4 cut(s) 13, 64, 69, 211
MseI TTAA 3 cut(s) 48, 59, 273
MspI CCGG 1 cut(s) 103
MwoI GCNNNNNNNGC 2 cut(s) 197, 370
NdeII GATC 5 cut(s) 85, 261, 348, 382, 477
NlaIII CATG 2 cut(s) 155, 258
NmuCI GTSAC 1 cut(s) 139
NspI RCATGY 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 202
PciI ACATGT 1 cut(s) 151
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PscI ACATGT 1 cut(s) 151
PshBI ATTAAT 1 cut(s) 48
PspXI VCTCGAGB 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 346
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
SaqAI TTAA 3 cut(s) 48, 59, 273
Sau3AI GATC 5 cut(s) 85, 261, 348, 382, 477
ScaI AGTACT 1 cut(s) 312
SchI GAGTC 1 cut(s) 26
SduI GDGCHC 1 cut(s) 366
SetI ASST 6 cut(s) 24, 42, 75, 240, 375, 466
SfaNI GCATC 1 cut(s) 313
Sfr274I CTCGAG 1 cut(s) 202
SlaI CTCGAG 1 cut(s) 202
SmlI CTYRAG 2 cut(s) 17, 202
SmoI CTYRAG 2 cut(s) 17, 202
Sse9I AATT 3 cut(s) 45, 60, 417
SsiI CCGC 1 cut(s) 266
SspMI CTAG 3 cut(s) 146, 197, 215
TaaI ACNGT 1 cut(s) 342
TaiI ACGT 1 cut(s) 240
TaqI TCGA 3 cut(s) 203, 321, 351
TasI AATT 3 cut(s) 45, 60, 417
TatI WGTACW 1 cut(s) 310
Tru1I TTAA 3 cut(s) 48, 59, 273
Tru9I TTAA 3 cut(s) 48, 59, 273
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 1 cut(s) 139
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 2 cut(s) 240, 291
TspGWI ACGGA 1 cut(s) 381
TspRI CASTG 1 cut(s) 144
VspI ATTAAT 1 cut(s) 48
XceI RCATGY 1 cut(s) 155
XhoI CTCGAG 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 234
XspI CTAG 3 cut(s) 146, 197, 215
ZrmI AGTACT 1 cut(s) 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.