Rw2G032370

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000160
Physical Location & Seq
Reverse (-)
53310785 .. 53313785
3001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032370.1

Sequence Viewer

Length: 2289 bp
ATGGAGGAAGAGGTAGAGGCCTTCCACTCTTTGTATGAGAGGCTATCCTCATACTTGGTGGAAACACAGGCCTATGATCTAGCCGCGACCTTAGAAATTGTTAATGAAGAGTTCACGGATCAAGAAGACGAGGAAGAAGAGATTCAACTTGCTCCAGCGGCATTGGATGACACACCTCCGAAGGTTAGGGACCCTACTGAAAAGGTCAATCTGGGAACAACTGATGAGCCTATAGAAGTGGCTATCAGCACTTACTTAGAGCCTAGCGAGAAACAGAGGCTCGTCGAATTATTGTTGGAATTCAAGGACTGCTTTGCGGAAAAATATGAGGACATGCCAGGCCTATCATCAGACTTGGTATGCCACCAACTACCAACGTTTCCTGATAAGAGGCCTGTGAAGCAAGAACCACGAAGAATGAACTCGGAAACCCAAGTTCTGGTCAAAGAGGAAGTTGAAAAGATGCACAAGTCAGGCATTATCAAAGTAGCCAAGTACAATCAGTGGCTATCCAATATAGTGCCTGTTCGCAAGAAGAATGGCAAGATGAGGGTTTGTGTAGATTACAGAGACCTTAATTTGGCTACACCTAAAGACGTCTACCCCATGCCGGTTGCTGACATGTTGGTAGACGCAGTTGCAGGGCACGAATTGTTGTCTTTCATGGATGGCACAGCGGGATATCACCAATTCCGGTCGCGGAGGAAGATAGACACAAGACCGCATTCCGTTGCCCTGGGTTTGCAGGACATGCAAGTTGCGGCATTACATGTTGTCCTTTACTACTGCATCATTGCTCAAACCGATCTAGTCAAATATATGCTGTCACGACCTATCTTGAGAGGCCGTATTGGCAAGTGGGTGCTCGCATTATCAGAATTCTCGCTACAGTATGTGCCACAGAAAGCAGTAAAGGGGCAAGCCATAGCAGACTTTCTGGCACATCACCCTATGCTAGATGTCCCCGCGGTTAGAGATTTAGAGGTCGCTGCCACAACTTTAGATCGCCCGGATTTAGCGTGCTTGCCAGAGTACGCCATGCTTTATCAAGACACAATCTCACTCCAGCCTTGGGTGTTATATTTTGACGGGTCAAGAACAGATACGCTAGCAGGAGCAGGGGTTGTCTTGGAAAACCCGGCCGGCGATCGATTTTCCTACTCTTTCCAGTTGGAGTTCCAGTGTACCAATAACCAAGCAGAATATGAGGCCCTCATTATAGGCCTAGAAGTACTACTGGAAATGGGAATCGGAGATGTACAAATACTTGGCGACTCTCTCTTGGTTATCAATCAGTTGTGCAACGAGTTCAGATGCAATAGCTTCACGTTGGTTCCTTATTGGAACAGGGCATTAGACCTGTTGGACCAGTTTGATAACATACATCTAGAGTACATTCCTCGCGAGCGAAATTTTGCAGCGAACGAGTTGGCCCAACTAGCAACAGGGGTAACATTGAGATATGGTGTGCGGGAGAGAATCCTTAAAGTCGAGCGTCGCACGCTTCCTTCATGGATGGCGAGAAGAGATCCTCCAGATGATACCTTAGTCGCGACCCTGGAACCCATTGACGTGGATTGGCGCATCCCTTTGATCGCTTATCTCAAGCAGCCAGATCCCATTGCCGACAGGAAGATTCGTTTTCTTGCACTAAATTACTTTCTTAGAGGTGACGAGCTGCGACGACGAGGCGAAGATGGCATAGACTTCAGATGTGTTTATGGTCGCGAAGCGAAACAACTCATGCGCGAAGTTCATTCCGGCATTTGCGGTGCTCACCAAGCAGGTCCAAAGATGCGATGGTTGCTCAGACGACATGGATATTTTTGGCCCAGTATCTTGAAGGATTGCATCGCATTCGCTAAAGGTTGTTTGGACTGCCAAGCTCATGGGCCGGTCCAGCATGTCCCTAATGTTCCAATGCAACCCATTATTAAGCCTTGGCCCGCAAGAGGATGGGCGCTTGATTTAATTGGGATGATTCACCCACATTCATCACTTCAGCACAAGTTCATCATCGTCGCGACCGATTTCTTTACAAAATGGGTCGAAGCAGAACCTTTGAAAGAAGCATCTGGTGGTACGTTGCGACAATTCCTATTTAGGAACATCATATGCAGATTTGGTATCCCAGAAGTTTTTGTTTCGGACCGGGGGGCAGCTTTCATGGGTGGTGAAGTTGATAAACTGGCAAAGGAATGGGGAATACAGTTCGTCCATTCCAGTCCTTATTATGCTCAGTCTAACGGTCAAGCGGAGGCCAGCAAAAAATCATCATCACTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

762

Amino Acids

86.71

Weight (kDa)

5.48

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 362 - 480 3.3e-20 Reverse transcriptase-like
Integrase_H2C2 PF17921 577 - 630 7.4e-08 Integrase zinc binding domain
rve PF00665 651 - 745 2.6e-09 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 1775
AccB7I CCANNNNNTGG 1 cut(s) 439
AccI GTMKAC 2 cut(s) 600, 630
AccII CGCG 8 cut(s) 86, 700, 968, 1404, 1553, 1728, 1749, 2024
AclI AACGTT 1 cut(s) 377
AclWI GGATC 3 cut(s) 126, 1523, 1610
AcoI YGGCCR 1 cut(s) 1140
AcsI RAATTY 3 cut(s) 299, 878, 1411
AcuI CTGAAG 2 cut(s) 1693, 1985
AcyI GRCGYC 1 cut(s) 597
AfaI GTAC 7 cut(s) 497, 1034, 1186, 1233, 1260, 1394, 2083
AfiI CCNNNNNNNGG 5 cut(s) 439, 580, 610, 1072, 1952
AflIII ACRYGT 2 cut(s) 621, 769
AgsI TTSAA 5 cut(s) 146, 304, 458, 1843, 2065
AjiI CACGTC 1 cut(s) 1573
AjnI CCWGG 3 cut(s) 337, 735, 1557
AluBI AGCT 4 cut(s) 1323, 1678, 1886, 2162
AluI AGCT 4 cut(s) 1323, 1678, 1886, 2162
Alw21I GWGCWC 2 cut(s) 867, 1777
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 3 cut(s) 126, 1523, 1610
ApeKI GCWGC 5 cut(s) 989, 1418, 1609, 1678, 2159
ApoI RAATTY 3 cut(s) 299, 878, 1411
Asp700I GAANNNNTTC 1 cut(s) 141
AspLEI GCGC 3 cut(s) 1584, 1749, 1963
AsuC2I CCSGG 3 cut(s) 1010, 1139, 2153
AsuHPI GGTGA 6 cut(s) 677, 938, 1682, 1769, 1976, 2186
AsuNHI GCTAGC 1 cut(s) 1108
AvaII GGWCC 5 cut(s) 190, 1366, 1787, 1897, 2149
BaeGI GKGCMC 1 cut(s) 648
BaeI ACNNNNGTAYC 2 cut(s) 1533, 1566
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 2 cut(s) 867, 1777
BbvI GCAGC 5 cut(s) 976, 1430, 1621, 1665, 2171
BccI CCATC 5 cut(s) 662, 1510, 1691, 1794, 1950
BceAI ACGGC 1 cut(s) 831
BcgI CGANNNNNNTGC 2 cut(s) 1840, 1874
BciT130I CCWGG 3 cut(s) 339, 737, 1559
BciVI GTATCC 1 cut(s) 2138
BcnI CCSGG 3 cut(s) 1010, 1139, 2153
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 8 cut(s) 80, 264, 809, 956, 1109, 1226, 1388, 1439
BfmI CTRYAG 2 cut(s) 231, 887
BfoI RGCGCY 1 cut(s) 1964
BfuAI ACCTGC 1 cut(s) 1775
BfuI GTATCC 1 cut(s) 2138
BglI GCCNNNNNGGC 1 cut(s) 852
BisI GCNGC 8 cut(s) 84, 159, 762, 990, 1419, 1610, 1679, 2160
BlsI GCNGC 8 cut(s) 85, 160, 763, 991, 1420, 1611, 1680, 2161
BmcAI AGTACT 1 cut(s) 1233
Bme1390I CCNGG 6 cut(s) 339, 737, 1010, 1139, 1559, 2153
Bme18I GGWCC 5 cut(s) 190, 1366, 1787, 1897, 2149
BmgBI CACGTC 1 cut(s) 1573
BmiI GGNNCC 4 cut(s) 191, 192, 1335, 1563
BmrFI CCNGG 6 cut(s) 339, 737, 1010, 1139, 1559, 2153
BmrI ACTGGG 1 cut(s) 1827
BmsI GCATC 7 cut(s) 453, 798, 1304, 1593, 1785, 1860, 2081
BmtI GCTAGC 1 cut(s) 1112
BmuI ACTGGG 1 cut(s) 1827
BpiI GAAGAC 1 cut(s) 132
BpmI CTGGAG 3 cut(s) 138, 1049, 1518
BpuEI CTTGAG 2 cut(s) 859, 1589
BpuMI CCSGG 3 cut(s) 1010, 1139, 2153
Bsa29I ATCGAT 1 cut(s) 1150
BsaHI GRCGYC 1 cut(s) 597
BsaI GGTCTC 1 cut(s) 564
BsaJI CCNNGG 7 cut(s) 735, 736, 966, 1070, 1557, 1940, 2152
BsaWI WCCGGW 1 cut(s) 693
BsaXI ACNNNNNCTCC 4 cut(s) 1107, 1137, 1166, 1196
Bsc4I CCNNNNNNNGG 5 cut(s) 439, 580, 610, 1072, 1952
Bse118I RCCGGY 3 cut(s) 610, 1142, 1894
Bse1I ACTGG 7 cut(s) 1168, 1180, 1242, 1369, 1833, 2193, 2223
Bse3DI GCAATG 2 cut(s) 792, 1620
BseBI CCWGG 3 cut(s) 339, 737, 1559
BseCI ATCGAT 1 cut(s) 1150
BseDI CCNNGG 7 cut(s) 735, 736, 966, 1070, 1557, 1940, 2152
BseGI GGATG 6 cut(s) 172, 673, 1521, 1584, 1961, 1983
BseLI CCNNNNNNNGG 5 cut(s) 439, 580, 610, 1072, 1952
BseMI GCAATG 2 cut(s) 792, 1620
BseMII CTCAG 2 cut(s) 1822, 2252
BseNI ACTGG 7 cut(s) 1168, 1180, 1242, 1369, 1833, 2193, 2223
BseSI GKGCMC 1 cut(s) 648
BseX3I CGGCCG 1 cut(s) 1140
BseXI GCAGC 5 cut(s) 976, 1430, 1621, 1665, 2171
Bsh1236I CGCG 8 cut(s) 86, 700, 968, 1404, 1553, 1728, 1749, 2024
Bsh1285I CGRYCG 4 cut(s) 698, 1143, 1150, 2028
BshVI ATCGAT 1 cut(s) 1150
BsiEI CGRYCG 4 cut(s) 698, 1143, 1150, 2028
BsiHKAI GWGCWC 2 cut(s) 867, 1777
BsiSI CCGG 8 cut(s) 611, 694, 1010, 1139, 1143, 1761, 1895, 2152
BslFI GGGAC 3 cut(s) 203, 947, 1892
BslI CCNNNNNNNGG 5 cut(s) 439, 580, 610, 1072, 1952
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 3 cut(s) 203, 947, 1892
BsmI GAATGC 2 cut(s) 724, 1856
Bso31I GGTCTC 1 cut(s) 564
Bsp1286I GDGCHC 3 cut(s) 648, 867, 1777
Bsp1407I TGTACA 1 cut(s) 1258
Bsp143I GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
Bsp68I TCGCGA 4 cut(s) 1404, 1553, 1728, 2024
BspCNI CTCAG 2 cut(s) 1821, 2251
BspDI ATCGAT 1 cut(s) 1150
BspFNI CGCG 8 cut(s) 86, 700, 968, 1404, 1553, 1728, 1749, 2024
BspLI GGNNCC 4 cut(s) 191, 192, 1335, 1563
BspMI ACCTGC 1 cut(s) 1775
BspOI GCTAGC 1 cut(s) 1112
BspPI GGATC 3 cut(s) 126, 1523, 1610
BspTNI GGTCTC 1 cut(s) 564
BsrDI GCAATG 2 cut(s) 792, 1620
BsrFI RCCGGY 3 cut(s) 610, 1142, 1894
BsrGI TGTACA 1 cut(s) 1258
BsrI ACTGG 7 cut(s) 1168, 1180, 1242, 1369, 1833, 2193, 2223
BssAI RCCGGY 3 cut(s) 610, 1142, 1894
BssECI CCNNGG 7 cut(s) 735, 736, 966, 1070, 1557, 1940, 2152
BssMI GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
BssNI GRCGYC 1 cut(s) 597
BssT1I CCWWGG 2 cut(s) 1070, 1940
Bst2UI CCWGG 3 cut(s) 339, 737, 1559
Bst4CI ACNGT 3 cut(s) 891, 2211, 2249
Bst6I CTCTTC 4 cut(s) 3, 102, 132, 1519
BstACI GRCGYC 1 cut(s) 597
BstAPI GCANNNNNTGC 1 cut(s) 751
BstAUI TGTACA 1 cut(s) 1258
BstDEI CTNAG 6 cut(s) 91, 256, 1546, 1664, 1808, 2238
BstDSI CCRYGG 1 cut(s) 966
BstF5I GGATG 6 cut(s) 172, 673, 1521, 1584, 1961, 1983
BstFNI CGCG 8 cut(s) 86, 700, 968, 1404, 1553, 1728, 1749, 2024
BstH2I RGCGCY 1 cut(s) 1964
BstHHI GCGC 3 cut(s) 1584, 1749, 1963
BstKTI GATC 8 cut(s) 79, 121, 808, 1006, 1150, 1531, 1596, 1618
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
BstMCI CGRYCG 4 cut(s) 698, 1143, 1150, 2028
BstNI CCWGG 3 cut(s) 339, 737, 1559
BstNSI RCATGY 5 cut(s) 337, 625, 754, 773, 1907
BstSCI CCNGG 6 cut(s) 337, 735, 1008, 1137, 1557, 2151
BstSFI CTRYAG 2 cut(s) 231, 887
BstSLI GKGCMC 1 cut(s) 648
BstUI CGCG 8 cut(s) 86, 700, 968, 1404, 1553, 1728, 1749, 2024
BstV1I GCAGC 5 cut(s) 976, 1430, 1621, 1665, 2171
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 2 cut(s) 1528, 1615
BstXI CCANNNNNNTGG 2 cut(s) 1573, 1889
BstYI RGATCY 2 cut(s) 1528, 1615
BstZI CGGCCG 1 cut(s) 1140
Bsu15I ATCGAT 1 cut(s) 1150
BsuI GTATCC 1 cut(s) 2138
BsuTUI ATCGAT 1 cut(s) 1150
BtgI CCRYGG 1 cut(s) 966
BtgZI GCGATG 2 cut(s) 1813, 1837
BtrI CACGTC 1 cut(s) 1573
BtsCI GGATG 6 cut(s) 172, 673, 1521, 1584, 1961, 1983
BtsIMutI CAGTG 2 cut(s) 509, 1187
BtuMI TCGCGA 4 cut(s) 1404, 1553, 1728, 2024
BveI ACCTGC 1 cut(s) 1775
CfoI GCGC 3 cut(s) 1584, 1749, 1963
Cfr10I RCCGGY 3 cut(s) 610, 1142, 1894
Cfr42I CCGCGG 1 cut(s) 969
ClaI ATCGAT 1 cut(s) 1150
CpoI CGGWCCG 1 cut(s) 2149
CseI GACGC 2 cut(s) 641, 1484
Csp6I GTAC 7 cut(s) 496, 1033, 1185, 1232, 1259, 1393, 2082
CspI CGGWCCG 1 cut(s) 2149
CviQI GTAC 7 cut(s) 496, 1033, 1185, 1232, 1259, 1393, 2082
DdeI CTNAG 6 cut(s) 91, 256, 1546, 1664, 1808, 2238
DpnI GATC 8 cut(s) 78, 120, 807, 1005, 1149, 1530, 1595, 1617
DpnII GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
EaeI YGGCCR 1 cut(s) 1140
EagI CGGCCG 1 cut(s) 1140
Eam1104I CTCTTC 4 cut(s) 3, 102, 132, 1519
EarI CTCTTC 4 cut(s) 3, 102, 132, 1519
EclXI CGGCCG 1 cut(s) 1140
Eco130I CCWWGG 2 cut(s) 1070, 1940
Eco147I AGGCCT 5 cut(s) 20, 71, 342, 394, 1224
Eco31I GGTCTC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 683
Eco47I GGWCC 5 cut(s) 190, 1366, 1787, 1897, 2149
Eco52I CGGCCG 1 cut(s) 1140
Eco57I CTGAAG 2 cut(s) 1693, 1985
EcoO109I RGGNCCY 2 cut(s) 190, 1210
EcoRI GAATTC 2 cut(s) 299, 878
EcoRII CCWGG 3 cut(s) 337, 735, 1557
EcoRV GATATC 1 cut(s) 683
EcoT14I CCWWGG 2 cut(s) 1070, 1940
ErhI CCWWGG 2 cut(s) 1070, 1940
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 3 cut(s) 203, 947, 1892
FauI CCCGC 4 cut(s) 670, 973, 1464, 1954
FauNDI CATATG 1 cut(s) 2114
FblI GTMKAC 2 cut(s) 600, 630
Fnu4HI GCNGC 8 cut(s) 84, 159, 762, 990, 1419, 1610, 1679, 2160
FokI GGATG 6 cut(s) 179, 680, 1528, 1571, 1968, 1990
Fsp4HI GCNGC 8 cut(s) 84, 159, 762, 990, 1419, 1610, 1679, 2160
FspBI CTAG 8 cut(s) 80, 264, 809, 956, 1109, 1226, 1388, 1439
GlaI GCGC 3 cut(s) 1583, 1748, 1962
GluI GCNGC 8 cut(s) 84, 159, 762, 990, 1419, 1610, 1679, 2160
GsuI CTGGAG 3 cut(s) 138, 1049, 1518
HaeII RGCGCY 1 cut(s) 1964
HapII CCGG 8 cut(s) 611, 694, 1010, 1139, 1143, 1761, 1895, 2152
HgaI GACGC 2 cut(s) 641, 1484
HhaI GCGC 3 cut(s) 1584, 1749, 1963
Hin1I GRCGYC 1 cut(s) 597
Hin6I GCGC 3 cut(s) 1582, 1747, 1961
HinP1I GCGC 3 cut(s) 1582, 1747, 1961
HinfI GANTC 6 cut(s) 142, 1248, 1274, 1479, 1636, 1981
HpaII CCGG 8 cut(s) 611, 694, 1010, 1139, 1143, 1761, 1895, 2152
HphI GGTGA 6 cut(s) 677, 938, 1682, 1769, 1976, 2186
Hpy166II GTNNAC 4 cut(s) 114, 601, 631, 1185
Hpy188I TCNGA 9 cut(s) 180, 352, 427, 877, 1253, 1313, 1712, 1811, 2149
Hpy8I GTNNAC 4 cut(s) 114, 601, 631, 1185
Hpy99I CGWCG 5 cut(s) 287, 1500, 1686, 1689, 2024
HpyAV CCTTC 4 cut(s) 31, 175, 1518, 1837
HpyCH4III ACNGT 3 cut(s) 891, 2211, 2249
HpyCH4IV ACGT 5 cut(s) 377, 597, 1328, 1572, 2084
HpyF3I CTNAG 6 cut(s) 91, 256, 1546, 1664, 1808, 2238
HpySE526I ACGT 5 cut(s) 377, 597, 1328, 1572, 2084
Hsp92I GRCGYC 1 cut(s) 597
HspAI GCGC 3 cut(s) 1582, 1747, 1961
KflI GGGWCCC 1 cut(s) 190
KroI GCCGGC 1 cut(s) 1142
KroNI GCCGGC 1 cut(s) 1144
KspI CCGCGG 1 cut(s) 969
Kzo9I GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
LmnI GCTCC 2 cut(s) 157, 1115
Lsp1109I GCAGC 5 cut(s) 976, 1430, 1621, 1665, 2171
LweI GCATC 7 cut(s) 453, 798, 1304, 1593, 1785, 1860, 2081
MaeI CTAG 8 cut(s) 80, 264, 809, 956, 1109, 1226, 1388, 1439
MaeII ACGT 5 cut(s) 377, 597, 1328, 1572, 2084
MaeIII GTNAC 3 cut(s) 825, 1450, 1670
MalI GATC 8 cut(s) 78, 120, 807, 1005, 1149, 1530, 1595, 1617
MboI GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
MflI RGATCY 2 cut(s) 1528, 1615
MhlI GDGCHC 3 cut(s) 648, 867, 1777
MlyI GAGTC 1 cut(s) 1268
MmeI TCCRAC 3 cut(s) 276, 1152, 1344
MroNI GCCGGC 1 cut(s) 1142
MroXI GAANNNNTTC 1 cut(s) 141
MseI TTAA 5 cut(s) 102, 576, 1485, 1935, 1970
MslI CAYNNNNRTG 1 cut(s) 1571
MspA1I CMGCKG 3 cut(s) 158, 677, 968
MspI CCGG 8 cut(s) 611, 694, 1010, 1139, 1143, 1761, 1895, 2152
MspR9I CCNGG 6 cut(s) 339, 737, 1010, 1139, 1559, 2153
Mva1269I GAATGC 2 cut(s) 724, 1856
MvaI CCWGG 3 cut(s) 339, 737, 1559
MvnI CGCG 8 cut(s) 86, 700, 968, 1404, 1553, 1728, 1749, 2024
NaeI GCCGGC 1 cut(s) 1144
NciI CCSGG 3 cut(s) 1010, 1139, 2153
NdeI CATATG 1 cut(s) 2114
NdeII GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
NgoMIV GCCGGC 1 cut(s) 1142
NheI GCTAGC 1 cut(s) 1108
NlaIV GGNNCC 4 cut(s) 191, 192, 1335, 1563
NmuCI GTSAC 2 cut(s) 825, 1670
NruI TCGCGA 4 cut(s) 1404, 1553, 1728, 2024
NspI RCATGY 5 cut(s) 337, 625, 754, 773, 1907
PasI CCCWGGG 1 cut(s) 736
PceI AGGCCT 5 cut(s) 20, 71, 342, 394, 1224
PciI ACATGT 2 cut(s) 621, 769
PcsI WCGNNNNNNNCGW 1 cut(s) 2025
PctI GAATGC 2 cut(s) 724, 1856
PdiI GCCGGC 1 cut(s) 1144
PdmI GAANNNNTTC 1 cut(s) 141
PfeI GAWTC 5 cut(s) 142, 1248, 1479, 1636, 1981
PflMI CCANNNNNTGG 1 cut(s) 439
PkrI GCNGC 8 cut(s) 85, 160, 763, 991, 1420, 1611, 1680, 2161
Ple19I CGATCG 1 cut(s) 1150
PleI GAGTC 1 cut(s) 1268
PpsI GAGTC 1 cut(s) 1268
PpuMI RGGWCCY 1 cut(s) 190
PscI ACATGT 2 cut(s) 621, 769
Psp1406I AACGTT 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 190
Psp6I CCWGG 3 cut(s) 337, 735, 1557
PspGI CCWGG 3 cut(s) 337, 735, 1557
PspN4I GGNNCC 4 cut(s) 191, 192, 1335, 1563
PspPPI RGGWCCY 1 cut(s) 190
PsuI RGATCY 2 cut(s) 1528, 1615
PvuI CGATCG 1 cut(s) 1150
RruI TCGCGA 4 cut(s) 1404, 1553, 1728, 2024
RsaI GTAC 7 cut(s) 497, 1034, 1186, 1233, 1260, 1394, 2083
RsaNI GTAC 7 cut(s) 496, 1033, 1185, 1232, 1259, 1393, 2082
RseI CAYNNNNRTG 1 cut(s) 1571
Rsr2I CGGWCCG 1 cut(s) 2149
RsrII CGGWCCG 1 cut(s) 2149
SacII CCGCGG 1 cut(s) 969
SaqAI TTAA 5 cut(s) 102, 576, 1485, 1935, 1970
SatI GCNGC 8 cut(s) 84, 159, 762, 990, 1419, 1610, 1679, 2160
Sau3AI GATC 8 cut(s) 76, 118, 805, 1003, 1147, 1528, 1593, 1615
ScaI AGTACT 1 cut(s) 1233
SchI GAGTC 1 cut(s) 1268
ScrFI CCNGG 6 cut(s) 339, 737, 1010, 1139, 1559, 2153
SduI GDGCHC 3 cut(s) 648, 867, 1777
SfaNI GCATC 7 cut(s) 453, 798, 1304, 1593, 1785, 1860, 2081
SfcI CTRYAG 2 cut(s) 231, 887
Sfr303I CCGCGG 1 cut(s) 969
SgrBI CCGCGG 1 cut(s) 969
SinI GGWCC 5 cut(s) 190, 1366, 1787, 1897, 2149
SmiMI CAYNNNNRTG 1 cut(s) 1571
SmlI CTYRAG 2 cut(s) 838, 1604
SmoI CTYRAG 2 cut(s) 838, 1604
SseBI AGGCCT 5 cut(s) 20, 71, 342, 394, 1224
SspMI CTAG 8 cut(s) 80, 264, 809, 956, 1109, 1226, 1388, 1439
StuI AGGCCT 5 cut(s) 20, 71, 342, 394, 1224
StyD4I CCNGG 6 cut(s) 337, 735, 1008, 1137, 1557, 2151
StyI CCWWGG 2 cut(s) 1070, 1940
TaaI ACNGT 3 cut(s) 891, 2211, 2249
TaiI ACGT 5 cut(s) 380, 600, 1331, 1575, 2087
TaqI TCGA 4 cut(s) 285, 1150, 1491, 2049
TaqII GACCGA 1 cut(s) 2042
TatI WGTACW 4 cut(s) 495, 1231, 1258, 1392
TauI GCSGC 3 cut(s) 86, 161, 764
TfiI GAWTC 5 cut(s) 142, 1248, 1479, 1636, 1981
Tru1I TTAA 5 cut(s) 102, 576, 1485, 1935, 1970
Tru9I TTAA 5 cut(s) 102, 576, 1485, 1935, 1970
TscAI CASTG 2 cut(s) 509, 1187
TseFI GTSAC 2 cut(s) 825, 1670
TseI GCWGC 5 cut(s) 989, 1418, 1609, 1678, 2159
Tsp45I GTSAC 2 cut(s) 825, 1670
TspDTI ATGAA 8 cut(s) 120, 434, 652, 1500, 1745, 1983, 2002, 2155
TspGWI ACGGA 2 cut(s) 131, 718
TspRI CASTG 2 cut(s) 509, 1187
Van91I CCANNNNNTGG 1 cut(s) 439
VpaK11BI GGWCC 5 cut(s) 190, 1366, 1787, 1897, 2149
XapI RAATTY 3 cut(s) 299, 878, 1411
XbaI TCTAGA 1 cut(s) 1387
XceI RCATGY 5 cut(s) 337, 625, 754, 773, 1907
XcmI CCANNNNNNNNNTGG 1 cut(s) 1797
XmiI GTMKAC 2 cut(s) 600, 630
XmnI GAANNNNTTC 1 cut(s) 141
XspI CTAG 8 cut(s) 80, 264, 809, 956, 1109, 1226, 1388, 1439
ZraI GACGTC 1 cut(s) 598
ZrmI AGTACT 1 cut(s) 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.