Rw4G031010

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000160
Physical Location & Seq
Reverse (-)
57828633 .. 57832241
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031010.1

Sequence Viewer

Length: 3609 bp
ATGGATTACGACCTCACTGAGGAGGGAATAGAAGCCTTCCACTCGATACACGAGCGTTTATCATCATACATGGTGGAAAAAGAGGCTTACGATCGCATTGCGACCTTAGAGGTCGTCAATGAAGAACTTGCTGATCCGGAAGATGAAGAGGAAATCCATATTCAGCTCGCTCCGGCGGCCCTGGACGATACACCTCCCAAGGTTAAAGACCCTACTAAGAAGGTCAACTTGGGTACCGTAAGTGAGCCTATGGAGGTCTCTGTCAGCACTTACCTAGAGCCTGGCGAGAAACAGAGGCTCATCGACCTATTGCTAGAGTTCAAGGATTGCTTCGCGGAGAAGTATGAGGACATGCCCGGCCAGTCACCAGACTTGGTTTGTCATCAGTTACCAACTCTTGCTGACAAGAGGCCCGTCAAGCAGGAACCTCGACGAATGAACGCGGAGACCCAGATTCTGGTGAAGGAAGAGGTAGAGAAAATGCATAAGTCAGGCATTATAAGGGTTGCCAAATATAACCAGTGGCTATCTAATATAGTGCCTGTCCGGAAGAAAAATGGCAAGATAAGAGTTTGCGTTGATTATAGAGACTTGAATCTTGCTACACCTAAAGACGTTTACCCCATGCCAGTCGCGGATATGTTGGTGGATGCGGTGGCGGGCCATGAATTGCTATCCTTCATGGATGGTACTGCAGGGTATCACCAAATTCCGGTCGCTGAAGAAGACAGACACAAGACGGCTTTCCGCTGCCCTGGCTTCGCGGGAGTTTTCGAATATGTAGTCATGCCTTTTGGCCTGAAGAACGCGGGTGCGACGTATCAGCGAGCCATGAATCTGATCTTCCACGACATACTTGGGAAGGTTCTAGAGATATACATTGACGACGTAGTTGTCAAGTCTCAGCAGAAAGGGAATCACATCGCGGATCTCAGAAAAGTATTCGAACGCATGCGCCGACATAAACTCAAGATGAACCCGGCCAAATGTGTGTTTGGGGTTCAAGCAGGGGACTTTCTGGGGTTTATAGTCCACCAGCGAGGAATTGAGGTCCCCGAGGATAAGGCAAGCGCTGTTATCAATGCATCTCCCCCACGGACGAAGAAGGAACTACAGCGTCTTTTGGGTAAGATTAATTTTCTCAGGCGTTTCATATCCAATTCTGCAAGGAAAATCCAGCCATTTTCACCTTTGCTGAGATTACACGGGCAGAATGAGTTTGTGTGGGAAGCCAAACATCAAGAGGCTTTTGACAGTATCAAGGCCTATTTGGCAAACCCGCCAGTCTTGGTTCCACCTAGAACCGGGGTCCCGTTAAAGCTCTACATTTTAGCGGCCAACGCCTCTATTGGCAGCCTGCTTGCTCAAGATGATGAGGACGGGGTCGAGCATGCTGTCTTTTATCTCAGCCGTACGCTGACAGACTGCGAGACAAGGTACACCCCTATGGAAAAGCTGTGCTTAACCTTATACTTTTCGGCGTGCAAGTTGCGACACTACATGTTATCTTTTACTACTTGCATCATCGCTCAGACCGATCTGGTCAAATATATGTTGTCCCGGCCTATCTTGCGAGGCCGCATTGGCAAATGGGTATTGGCACTTTCAGAGTTTTCGCTCCAATATGTACCCCAGAAGGCGGTGAAAGGGCAAGCGATTGCGGATTTTCTGGCACACCATCCTCCGCTAGAAATACCTGTACTAAAGGAGTTAGAGATTGCCTGCAACGCTACGACTCGACCAGATAAAGCGTGCATCCCTGAATACGAGGTCTGGTATCAGGCTACAATCTTTCTACAGCCCTGGGTGTTATTTTTTGATGGTTCAAGAACGGACGCTATGGCTGGCGCGGGGATTGTTTTAGAGAACCCCGCAGGTGATCGCTTCTCTTATTCTTTCCAGCTCACATTCCAATGTACTAATAATCAGGCAGAATATGAGGCTCTTATCATTGGACTCGAAGTCTTGTTGGAAATGGGCGTCCGGGACGTTCAGATTCGCGGCGATTCCCAGCTCGTCATCAATCAGCTTCTGGAAAAATATCGTTGTACGAGTTGGCTGCTCATCCCATACCTGAATCGCGCCATTGAGCTTCTGGACCAATTTACAGACGTTGATATGGAGCATATACCACGTGAACGTAACTTTGCTGCTAATGAGCTCGCTCAGTTGGCTACAGGCATCACCTTGAAATACGGAGTACGCGAGCGCATTCTGAAAGTGGAGCGTCGCACACTACCTTCATGGCTTGCTCGACCTGATCCTCCAGATGATCCCGTGGTTGCGGTACTAGAGCCCATTGATGTCGATTGGCGGATTCCACTAATTGAATACCCCAAACAACCAGACTCCAGCGCTGATAGGAGGATTCGTTTCCTCGCATTGAATTACTTCCTTAGAAGCGATGAACTGCGCAGATGTGGAGAAGATGGTATAGATTTTCGATGTGTCTACGGTCGCGAAGCCAAGCGATTGATGCGTGAGGTACACACGGAGATATGTGGGTCCCATCAGGCTGGACCTAAGATGCGTTGGCTCCTCCGACGTCATGGTTATTATTGGCCCAGCATTTTGAAGGATTGTATTGCATTCGCCAAAGGCTGTGCGGACTGTCAAGCACACGGTCCTGTCCAGCATGTTCCTAACATTCCTATGCAACCGATCATAAAACCTTGGCCTGCGCGAGGCTGGGCATTGGACTTGATCGGGATGATCCATCCTCATTCTTCCCTACAGCACAAGTTTATCATCGTCGCCACTGATTACTTCACGAAATGGGTTGAAGCAGAGCCTCTAAAGGAGGCCTCCGGCCCTACCATTCGCCAGTTTATTTTCCGTAATATTCTCTGCCGATTTGGTATCCCTGAAGTGCTGGTGTCAGACAGGGGGGCAGCGTTCATGGGAGGCCCCGTGGAGGAGCTGGTAAATGATTTTGGCATTCAATTCATACATAGTACTCCGTACTATGCTCAATCTAATGGTCAGGCGGAGGCGAGCAACAAGACAATCATCACCTTACTGAAGAAGATGTTGGTGGAGAATCCTCGACAGTGGCATGATACTTTGTACGAGACACTTTGGGCCTATCGCACTTCTAAACGTAACCCCACTGCCACGACGCCGTACGCGCTTATGTTTGGACATGACGCTGTCTTACCATTAGAGATTAATGTTCACTCCCTACGAGTCCAAGAACAGCATCATTTGATTGGCGAAGATTATGTTCAAGCAATGTGGCAGGAGCACGAAGACTTAAGCGACCAGCGCTTGGCCGCTCTGGACAATTTGGTGATGGAAAAACAGCGTATCGCCCGCGCCTATGATAAGAGGACGCGGGGGCGCAGTTTCAAAGAAGGTGACCTCGTTTGGAAGGCCATTTTACCCTTCGGTGAAAAGGTGACCGGTCGCGGTAAATGGACGCCGCGATGGGAGGGACCCTTTGTTATCCACCGAATATTGGAGCGCGGCGCTTTTCACCTCAAAGACATCGACGGTGATATCCATCGCAACCCTATTAACGGTCGTTTTCTGAAGAAATACTACCCGAGTGTCTGGGAGTTTGACGATCCTCCCGACCCTGTTCTTGCTCAGGCTGGGGGGCAACCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

1202

Amino Acids

137.41

Weight (kDa)

6.46

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 182 - 344 1.6e-31 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 409 - 503 1.1e-19 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 438 - 538 7.8e-16 RNase H-like domain found in reverse transcriptase
RNase_H PF00075 603 - 716 7.4e-06 RNase H
RVT_3 PF13456 606 - 724 2.8e-20 Reverse transcriptase-like
Integrase_H2C2 PF17921 821 - 874 2.2e-07 Integrase zinc binding domain
rve PF00665 895 - 989 2.4e-07 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AarI CACCTGC 1 cut(s) 1865
AasI GACNNNNNNGTC 1 cut(s) 893
AatII GACGTC 1 cut(s) 2548
Acc16I TGCGCA 1 cut(s) 2414
Acc36I ACCTGC 1 cut(s) 1865
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 1 cut(s) 233
AccB7I CCANNNNNTGG 2 cut(s) 457, 3268
AccBSI CCGCTC 1 cut(s) 3275
AccI GTMKAC 1 cut(s) 2451
AccIII TCCGGA 2 cut(s) 136, 546
AclWI GGATC 6 cut(s) 128, 936, 2255, 2267, 2707, 3560
AcoI YGGCCR 4 cut(s) 358, 981, 1335, 3270
AcsI RAATTY 1 cut(s) 708
AcuI CTGAAG 5 cut(s) 741, 821, 2886, 3041, 3551
AcvI CACGTG 1 cut(s) 2135
AcyI GRCGYC 4 cut(s) 1980, 2545, 3119, 3419
AfeI AGCGCT 3 cut(s) 1072, 2356, 3266
AflII CTTAAG 1 cut(s) 3253
AflIII ACRYGT 1 cut(s) 1500
AgeI ACCGGT 1 cut(s) 3401
AhdI GACNNNNNGTC 1 cut(s) 1961
AjnI CCWGG 4 cut(s) 180, 280, 754, 1802
AjuI GAANNNNNNNTTGG 4 cut(s) 212, 244, 3014, 3046
AluBI AGCT 9 cut(s) 166, 1321, 1456, 1903, 2014, 2029, 2092, 2161, 2920
AluI AGCT 9 cut(s) 166, 1321, 1456, 1903, 2014, 2029, 2092, 2161, 2920
Alw21I GWGCWC 2 cut(s) 2163, 3246
Alw26I GTCTC 6 cut(s) 262, 440, 582, 906, 1424, 3065
AlwI GGATC 6 cut(s) 128, 936, 2255, 2267, 2707, 3560
AlwNI CAGNNNCTG 2 cut(s) 457, 2032
Ama87I CYCGRG 2 cut(s) 1055, 3544
Aor13HI TCCGGA 2 cut(s) 136, 546
Aor51HI AGCGCT 3 cut(s) 1072, 2356, 3266
ApeKI GCWGC 5 cut(s) 750, 1353, 2059, 2150, 2891
ApoI RAATTY 1 cut(s) 708
ArsI GACNNNNNNTTYG 2 cut(s) 768, 800
AseI ATTAAT 2 cut(s) 1134, 3168
AsiGI ACCGGT 1 cut(s) 3401
Asp700I GAANNNNTTC 1 cut(s) 2390
Asp718I GGTACC 1 cut(s) 233
AsuC2I CCSGG 5 cut(s) 357, 980, 1306, 1561, 1985
AsuII TTCGAA 2 cut(s) 774, 945
AvaI CYCGRG 2 cut(s) 1055, 3544
AvaII GGWCC 7 cut(s) 1051, 1309, 2098, 2505, 2519, 2624, 3434
BaeI ACNNNNGTAYC 2 cut(s) 217, 250
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 2 cut(s) 2163, 2298
BarI GAAGNNNNNNTAC 2 cut(s) 3524, 3556
BauI CACGAG 1 cut(s) 50
BbrPI CACGTG 1 cut(s) 2135
BbsI GAAGAC 2 cut(s) 732, 3255
Bbv12I GWGCWC 2 cut(s) 2163, 3246
BbvI GCAGC 5 cut(s) 737, 1365, 2046, 2137, 2903
BccI CCATC 9 cut(s) 680, 1686, 1814, 2423, 2517, 2724, 3286, 3420, 3510
BceAI ACGGC 3 cut(s) 756, 1395, 3106
BcgI CGANNNNNNTGC 6 cut(s) 80, 114, 410, 444, 2041, 2075
BciT130I CCWGG 4 cut(s) 182, 282, 756, 1804
BciVI GTATCC 1 cut(s) 2870
BcnI CCSGG 5 cut(s) 357, 980, 1306, 1561, 1985
BcoDI GTCTC 6 cut(s) 262, 440, 582, 906, 1424, 3065
BfaI CTAG 6 cut(s) 275, 314, 869, 1299, 1688, 2291
BfmI CTRYAG 5 cut(s) 693, 1112, 1796, 2175, 2732
BfoI RGCGCY 4 cut(s) 1074, 2358, 3268, 3471
BfrI CTTAAG 1 cut(s) 3253
BfuAI ACCTGC 1 cut(s) 1865
BfuI GTATCC 1 cut(s) 2870
BglI GCCNNNNNGGC 2 cut(s) 1271, 1584
BmcAI AGTACT 1 cut(s) 2956
Bme1390I CCNGG 9 cut(s) 182, 282, 357, 756, 980, 1306, 1561, 1804, 1985
Bme18I GGWCC 7 cut(s) 1051, 1309, 2098, 2505, 2519, 2624, 3434
BmeRI GACNNNNNGTC 1 cut(s) 1961
BmeT110I CYCGRG 2 cut(s) 1055, 3544
BmrFI CCNGG 9 cut(s) 182, 282, 357, 756, 980, 1306, 1561, 1804, 1985
BmsI GCATC 8 cut(s) 640, 1094, 1530, 1764, 2190, 2466, 2517, 3208
BpiI GAAGAC 2 cut(s) 732, 3255
BpmI CTGGAG 2 cut(s) 2250, 2335
Bpu10I CCTNAGC 1 cut(s) 3588
Bpu14I TTCGAA 2 cut(s) 774, 945
BpuEI CTTGAG 2 cut(s) 953, 1350
BpuMI CCSGG 5 cut(s) 357, 980, 1306, 1561, 1985
BsaAI YACGTR 1 cut(s) 2135
BsaBI GATNNNNATC 1 cut(s) 3501
BsaHI GRCGYC 4 cut(s) 1980, 2545, 3119, 3419
BsaI GGTCTC 2 cut(s) 262, 440
BsaWI WCCGGW 4 cut(s) 136, 546, 712, 3401
BsaXI ACNNNNNCTCC 4 cut(s) 2356, 2386, 2895, 2925
Bse118I RCCGGY 1 cut(s) 3401
Bse1I ACTGG 5 cut(s) 361, 520, 629, 1283, 2824
Bse3DI GCAATG 2 cut(s) 96, 3237
Bse8I GATNNNNATC 1 cut(s) 3501
BseAI TCCGGA 2 cut(s) 136, 546
BseBI CCWGG 4 cut(s) 182, 282, 756, 1804
BseGI GGATG 7 cut(s) 655, 691, 1678, 1755, 2064, 2715, 2716
BseJI GATNNNNATC 1 cut(s) 3501
BseMI GCAATG 2 cut(s) 96, 3237
BseMII CTCAG 9 cut(s) 9, 917, 946, 1156, 1187, 1420, 1544, 2180, 3602
BseNI ACTGG 5 cut(s) 361, 520, 629, 1283, 2824
BseRI GAGGAG 3 cut(s) 35, 2528, 2930
BseXI GCAGC 5 cut(s) 737, 1365, 2046, 2137, 2903
BseYI CCCAGC 4 cut(s) 2010, 2564, 2688, 3593
Bsh1285I CGRYCG 5 cut(s) 94, 717, 2458, 3406, 3523
BshNI GGYRCC 1 cut(s) 233
BshTI ACCGGT 1 cut(s) 3401
BsiEI CGRYCG 5 cut(s) 94, 717, 2458, 3406, 3523
BsiHKAI GWGCWC 2 cut(s) 2163, 3246
BsiHKCI CYCGRG 2 cut(s) 1055, 3544
BsiWI CGTACG 2 cut(s) 1412, 3123
BslFI GGGAC 7 cut(s) 1025, 1037, 1295, 1543, 2000, 2491, 3447
BsmAI GTCTC 6 cut(s) 262, 440, 582, 906, 1424, 3065
BsmFI GGGAC 7 cut(s) 1025, 1037, 1295, 1543, 2000, 2491, 3447
BsmI GAATGC 3 cut(s) 2211, 2588, 2937
Bso31I GGTCTC 2 cut(s) 262, 440
BsoBI CYCGRG 2 cut(s) 1055, 3544
Bsp119I TTCGAA 2 cut(s) 774, 945
Bsp1286I GDGCHC 3 cut(s) 2163, 2298, 3246
Bsp13I TCCGGA 2 cut(s) 136, 546
Bsp68I TCGCGA 1 cut(s) 2460
BspCNI CTCAG 9 cut(s) 10, 916, 945, 1155, 1188, 1419, 1543, 2179, 3601
BspEI TCCGGA 2 cut(s) 136, 546
BspMAI CTGCAG 1 cut(s) 697
BspMI ACCTGC 1 cut(s) 1865
BspPI GGATC 6 cut(s) 128, 936, 2255, 2267, 2707, 3560
BspT104I TTCGAA 2 cut(s) 774, 945
BspT107I GGYRCC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 3253
BspTNI GGTCTC 2 cut(s) 262, 440
BsrBI CCGCTC 1 cut(s) 3275
BsrDI GCAATG 2 cut(s) 96, 3237
BsrFI RCCGGY 1 cut(s) 3401
BsrI ACTGG 5 cut(s) 361, 520, 629, 1283, 2824
BssAI RCCGGY 1 cut(s) 3401
BssNI GRCGYC 4 cut(s) 1980, 2545, 3119, 3419
BssSI CACGAG 1 cut(s) 50
BssT1I CCWWGG 2 cut(s) 198, 2672
Bst2BI CACGAG 1 cut(s) 50
Bst2UI CCWGG 4 cut(s) 182, 282, 756, 1804
Bst4CI ACNGT 9 cut(s) 238, 1256, 2456, 2612, 2624, 3051, 3494, 3521, 3606
Bst6I CTCTTC 2 cut(s) 141, 462
BstACI GRCGYC 4 cut(s) 1980, 2545, 3119, 3419
BstAFI CTTAAG 1 cut(s) 3253
BstBAI YACGTR 1 cut(s) 2135
BstBI TTCGAA 2 cut(s) 774, 945
BstDSI CCRYGG 3 cut(s) 1094, 2277, 2910
BstEII GGTNACC 2 cut(s) 3356, 3397
BstENI CCTNNNNNAGG 2 cut(s) 17, 2682
BstF5I GGATG 7 cut(s) 655, 691, 1678, 1755, 2064, 2715, 2716
BstH2I RGCGCY 4 cut(s) 1074, 2358, 3268, 3471
BstMAI GTCTC 6 cut(s) 262, 440, 582, 906, 1424, 3065
BstMCI CGRYCG 5 cut(s) 94, 717, 2458, 3406, 3523
BstNI CCWGG 4 cut(s) 182, 282, 756, 1804
BstNSI RCATGY 5 cut(s) 355, 955, 1394, 1504, 2639
BstPI GGTNACC 2 cut(s) 3356, 3397
BstSCI CCNGG 9 cut(s) 180, 280, 355, 754, 978, 1304, 1559, 1802, 1983
BstSFI CTRYAG 5 cut(s) 693, 1112, 1796, 2175, 2732
BstV1I GCAGC 5 cut(s) 737, 1365, 2046, 2137, 2903
BstV2I GAAGAC 2 cut(s) 732, 3255
BstX2I RGATCY 1 cut(s) 928
BstXI CCANNNNNNTGG 1 cut(s) 2516
BstYI RGATCY 1 cut(s) 928
BsuI GTATCC 1 cut(s) 2870
BtgI CCRYGG 3 cut(s) 1094, 2277, 2910
BtgZI GCGATG 5 cut(s) 907, 1510, 2418, 3439, 3488
BtsCI GGATG 7 cut(s) 655, 691, 1678, 1755, 2064, 2715, 2716
BtsI GCAGTG 1 cut(s) 3108
BtsIMutI CAGTG 5 cut(s) 15, 527, 2757, 3056, 3108
BtuMI TCGCGA 1 cut(s) 2460
BveI ACCTGC 1 cut(s) 1865
CaiI CAGNNNCTG 2 cut(s) 457, 2032
Cfr10I RCCGGY 1 cut(s) 3401
CseI GACGC 8 cut(s) 1106, 1844, 1969, 2216, 3127, 3155, 3340, 3427
CspAI ACCGGT 1 cut(s) 3401
CspCI CAANNNNNGTGG 2 cut(s) 3215, 3250
DrdI GACNNNNNNGTC 1 cut(s) 893
DriI GACNNNNNGTC 1 cut(s) 1961
DseDI GACNNNNNNGTC 1 cut(s) 893
EaeI YGGCCR 4 cut(s) 358, 981, 1335, 3270
Eam1104I CTCTTC 2 cut(s) 141, 462
Eam1105I GACNNNNNGTC 1 cut(s) 1961
EarI CTCTTC 2 cut(s) 141, 462
EciI GGCGGA 2 cut(s) 2329, 3002
Ecl136II GAGCTC 1 cut(s) 2161
Eco130I CCWWGG 2 cut(s) 198, 2672
Eco147I AGGCCT 2 cut(s) 1265, 2804
Eco24I GRGCYC 2 cut(s) 2163, 2298
Eco31I GGTCTC 2 cut(s) 262, 440
Eco32I GATATC 1 cut(s) 3499
Eco47I GGWCC 7 cut(s) 1051, 1309, 2098, 2505, 2519, 2624, 3434
Eco47III AGCGCT 3 cut(s) 1072, 2356, 3266
Eco53kI GAGCTC 1 cut(s) 2161
Eco57I CTGAAG 5 cut(s) 741, 821, 2886, 3041, 3551
Eco72I CACGTG 1 cut(s) 2135
Eco88I CYCGRG 2 cut(s) 1055, 3544
Eco91I GGTNACC 2 cut(s) 3356, 3397
EcoICRI GAGCTC 1 cut(s) 2161
EcoNI CCTNNNNNAGG 2 cut(s) 17, 2682
EcoO109I RGGNCCY 5 cut(s) 1051, 1309, 2505, 2906, 3434
EcoO65I GGTNACC 2 cut(s) 3356, 3397
EcoRII CCWGG 4 cut(s) 180, 280, 754, 1802
EcoRV GATATC 1 cut(s) 3499
EcoT14I CCWWGG 2 cut(s) 198, 2672
EcoT22I ATGCAT 2 cut(s) 486, 1087
EcoT38I GRGCYC 2 cut(s) 2163, 2298
ErhI CCWWGG 2 cut(s) 198, 2672
FalI AAGNNNNNCTT 6 cut(s) 212, 244, 314, 346, 1445, 1477
FaqI GGGAC 7 cut(s) 1025, 1037, 1295, 1543, 2000, 2491, 3447
FauI CCCGC 8 cut(s) 652, 757, 802, 1287, 1843, 1879, 3320, 3327
FblI GTMKAC 1 cut(s) 2451
FokI GGATG 7 cut(s) 662, 698, 1665, 1742, 2051, 2703, 2722
FriOI GRGCYC 2 cut(s) 2163, 2298
FspBI CTAG 6 cut(s) 275, 314, 869, 1299, 1688, 2291
FspI TGCGCA 1 cut(s) 2414
GsaI CCCAGC 4 cut(s) 2014, 2568, 2692, 3597
GsuI CTGGAG 2 cut(s) 2250, 2335
HaeII RGCGCY 4 cut(s) 1074, 2358, 3268, 3471
HgaI GACGC 8 cut(s) 1106, 1844, 1969, 2216, 3127, 3155, 3340, 3427
Hin1I GRCGYC 4 cut(s) 1980, 2545, 3119, 3419
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
Hpy166II GTNNAC 8 cut(s) 226, 619, 1033, 1440, 2138, 2452, 2488, 3175
Hpy188I TCNGA 9 cut(s) 840, 935, 1533, 1609, 1995, 2217, 2543, 2881, 3531
Hpy8I GTNNAC 8 cut(s) 226, 619, 1033, 1440, 2138, 2452, 2488, 3175
Hpy99I CGWCG 8 cut(s) 435, 820, 890, 2232, 2547, 2756, 3121, 3494
HpyCH4III ACNGT 9 cut(s) 238, 1256, 2456, 2612, 2624, 3051, 3494, 3521, 3606
HpyCH4IV ACGT 9 cut(s) 615, 818, 888, 1989, 2112, 2134, 2140, 2545, 3100
HpySE526I ACGT 9 cut(s) 615, 818, 888, 1989, 2112, 2134, 2140, 2545, 3100
Hsp92I GRCGYC 4 cut(s) 1980, 2545, 3119, 3419
KflI GGGWCCC 3 cut(s) 1309, 2505, 3434
Kpn2I TCCGGA 2 cut(s) 136, 546
KpnI GGTACC 1 cut(s) 237
LmnI GCTCC 8 cut(s) 175, 1623, 2122, 2224, 2541, 2917, 3241, 3460
Lsp1109I GCAGC 5 cut(s) 737, 1365, 2046, 2137, 2903
LweI GCATC 8 cut(s) 640, 1094, 1530, 1764, 2190, 2466, 2517, 3208
MaeI CTAG 6 cut(s) 275, 314, 869, 1299, 1688, 2291
MaeII ACGT 9 cut(s) 615, 818, 888, 1989, 2112, 2134, 2140, 2545, 3100
MaeIII GTNAC 6 cut(s) 363, 387, 2141, 3101, 3356, 3397
MbiI CCGCTC 1 cut(s) 3275
MflI RGATCY 1 cut(s) 928
MhlI GDGCHC 3 cut(s) 2163, 2298, 3246
MlyI GAGTC 4 cut(s) 1729, 1950, 2342, 3195
MmeI TCCRAC 2 cut(s) 1950, 2566
Mph1103I ATGCAT 2 cut(s) 486, 1087
MroI TCCGGA 2 cut(s) 136, 546
MroXI GAANNNNTTC 1 cut(s) 2390
MseI TTAA 7 cut(s) 204, 1134, 1316, 1463, 3168, 3254, 3516
MslI CAYNNNNRTG 3 cut(s) 1445, 1912, 2651
MspA1I CMGCKG 1 cut(s) 750
MspCI CTTAAG 1 cut(s) 3253
MspR9I CCNGG 9 cut(s) 182, 282, 357, 756, 980, 1306, 1561, 1804, 1985
MteI GCGCNGCGC 1 cut(s) 3466
Mva1269I GAATGC 3 cut(s) 2211, 2588, 2937
MvaI CCWGG 4 cut(s) 182, 282, 756, 1804
NciI CCSGG 5 cut(s) 357, 980, 1306, 1561, 1985
NmuCI GTSAC 3 cut(s) 363, 3356, 3397
NruI TCGCGA 1 cut(s) 2460
NsbI TGCGCA 1 cut(s) 2414
NsiI ATGCAT 2 cut(s) 486, 1087
NspI RCATGY 5 cut(s) 355, 955, 1394, 1504, 2639
NspV TTCGAA 2 cut(s) 774, 945
PaeI GCATGC 2 cut(s) 955, 1394
PaqCI CACCTGC 1 cut(s) 1865
PasI CCCWGGG 1 cut(s) 1803
PceI AGGCCT 2 cut(s) 1265, 2804
PciI ACATGT 1 cut(s) 1500
PcsI WCGNNNNNNNCGW 4 cut(s) 955, 1533, 2202, 3570
PctI GAATGC 3 cut(s) 2211, 2588, 2937
PdmI GAANNNNTTC 1 cut(s) 2390
Pfl23II CGTACG 2 cut(s) 1412, 3123
PflFI GACNNNGTC 2 cut(s) 1382, 3149
PflMI CCANNNNNTGG 2 cut(s) 457, 3268
PfoI TCCNGGA 1 cut(s) 1983
PinAI ACCGGT 1 cut(s) 3401
Ple19I CGATCG 1 cut(s) 94
PleI GAGTC 4 cut(s) 1729, 1950, 2342, 3194
PmaCI CACGTG 1 cut(s) 2135
PmlI CACGTG 1 cut(s) 2135
PpsI GAGTC 4 cut(s) 1729, 1950, 2342, 3194
Ppu21I YACGTR 1 cut(s) 2135
PpuMI RGGWCCY 4 cut(s) 1051, 1309, 2505, 3434
PscI ACATGT 1 cut(s) 1500
PshBI ATTAAT 2 cut(s) 1134, 3168
PsiI TTATAA 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 2163
Psp5II RGGWCCY 4 cut(s) 1051, 1309, 2505, 3434
Psp6I CCWGG 4 cut(s) 180, 280, 754, 1802
PspCI CACGTG 1 cut(s) 2135
PspEI GGTNACC 2 cut(s) 3356, 3397
PspFI CCCAGC 4 cut(s) 2010, 2564, 2688, 3593
PspGI CCWGG 4 cut(s) 180, 280, 754, 1802
PspLI CGTACG 2 cut(s) 1412, 3123
PspPPI RGGWCCY 4 cut(s) 1051, 1309, 2505, 3434
PstI CTGCAG 1 cut(s) 697
PstNI CAGNNNCTG 2 cut(s) 457, 2032
PsuI RGATCY 1 cut(s) 928
PsyI GACNNNGTC 2 cut(s) 1382, 3149
PvuI CGATCG 1 cut(s) 94
RruI TCGCGA 1 cut(s) 2460
RseI CAYNNNNRTG 3 cut(s) 1445, 1912, 2651
SacI GAGCTC 1 cut(s) 2163
SaqAI TTAA 7 cut(s) 204, 1134, 1316, 1463, 3168, 3254, 3516
ScaI AGTACT 1 cut(s) 2956
SchI GAGTC 4 cut(s) 1729, 1950, 2342, 3195
ScrFI CCNGG 9 cut(s) 182, 282, 357, 756, 980, 1306, 1561, 1804, 1985
SduI GDGCHC 3 cut(s) 2163, 2298, 3246
SfaNI GCATC 8 cut(s) 640, 1094, 1530, 1764, 2190, 2466, 2517, 3208
SfcI CTRYAG 5 cut(s) 693, 1112, 1796, 2175, 2732
SfuI TTCGAA 2 cut(s) 774, 945
SinI GGWCC 7 cut(s) 1051, 1309, 2098, 2505, 2519, 2624, 3434
SmiMI CAYNNNNRTG 3 cut(s) 1445, 1912, 2651
SmlI CTYRAG 3 cut(s) 968, 1365, 3253
SmoI CTYRAG 3 cut(s) 968, 1365, 3253
SphI GCATGC 2 cut(s) 955, 1394
SseBI AGGCCT 2 cut(s) 1265, 2804
SspI AATATT 2 cut(s) 2842, 3456
SspMI CTAG 6 cut(s) 275, 314, 869, 1299, 1688, 2291
SstI GAGCTC 1 cut(s) 2163
StuI AGGCCT 2 cut(s) 1265, 2804
StyD4I CCNGG 9 cut(s) 180, 280, 355, 754, 978, 1304, 1559, 1802, 1983
StyI CCWWGG 2 cut(s) 198, 2672
TaaI ACNGT 9 cut(s) 238, 1256, 2456, 2612, 2624, 3051, 3494, 3521, 3606
TaiI ACGT 9 cut(s) 618, 821, 891, 1992, 2115, 2137, 2143, 2548, 3103
TaqII GACCGA 1 cut(s) 1550
TatI WGTACW 3 cut(s) 1699, 1916, 2954
TauI GCSGC 7 cut(s) 179, 1337, 1581, 2004, 3275, 3424, 3468
Tru1I TTAA 7 cut(s) 204, 1134, 1316, 1463, 3168, 3254, 3516
Tru9I TTAA 7 cut(s) 204, 1134, 1316, 1463, 3168, 3254, 3516
TscAI CASTG 5 cut(s) 22, 527, 2764, 3056, 3115
TseFI GTSAC 3 cut(s) 363, 3356, 3397
TseI GCWGC 5 cut(s) 750, 1353, 2059, 2150, 2891
Tsp45I GTSAC 3 cut(s) 363, 3356, 3397
TspGWI ACGGA 6 cut(s) 1111, 1847, 2211, 2507, 2825, 2949
TspRI CASTG 5 cut(s) 22, 527, 2764, 3056, 3115
Tth111I GACNNNGTC 2 cut(s) 1382, 3149
Van91I CCANNNNNTGG 2 cut(s) 457, 3268
Vha464I CTTAAG 1 cut(s) 3253
VpaK11BI GGWCC 7 cut(s) 1051, 1309, 2098, 2505, 2519, 2624, 3434
VspI ATTAAT 2 cut(s) 1134, 3168
XagI CCTNNNNNAGG 2 cut(s) 17, 2682
XapI RAATTY 1 cut(s) 708
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 5 cut(s) 355, 955, 1394, 1504, 2639
XcmI CCANNNNNNNNNTGG 2 cut(s) 854, 2092
XmiI GTMKAC 1 cut(s) 2451
XmnI GAANNNNTTC 1 cut(s) 2390
XspI CTAG 6 cut(s) 275, 314, 869, 1299, 1688, 2291
ZraI GACGTC 1 cut(s) 2546
ZrmI AGTACT 1 cut(s) 2956
Zsp2I ATGCAT 2 cut(s) 486, 1087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.