Rw6G001390

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000160
Physical Location & Seq
Forward (+)
2146863 .. 2149025
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G001390.1

Sequence Viewer

Length: 1641 bp
ATGGAAAAGTTGTGCTTAACCCTATACTTTTCGGCGTGCAAGTTGCGACATTACATGTTATCCTTTACTACTTGCACCATCGCTCAGACCGATTTGGTCAAATATATGTTGTCCCGGCCTATCTTGAGAGGCCGCATTGGTAAATGGGTATTGGCACTGTCAGAGTTTTCTCTCCAGTATGTACCCCAGAAGGCGGTCAAAGGACAGGCTATCGCAGATTTTCTAGCACATCATCCTCCGCTAGAACTTCCTGTACTGAAAGAGTTGGAAATTGCCTGCAACACCACGACTCGACCAGATAACGCATGCATCCCTGAATATGAGGTCTGGTATCAGGCTACAATCTCTCTACAGCCCTGGGTGTTATTCTTTGATGGCTCAAGAACAGATACTCTGGCCGGCGCAGGGATTGTTCTGGAGAACCCTGCCGGTGATCGTTTCTCTTATTCTTTCCAGCTCACGTTCCAATGTACTAATAATCAGGCCGAATATGAAGCTCTAATCATTGGACTCGAAGTCTTGTTGGAAATGGGGGTCCGGGACGTTCAGATTCGCGGCGATTCTCAGCTCGTTATTAATCAACTTCTGGAAAAATATCGCTGTACGAGTTGGCTGCTCATCCCATACCTGAATCGCGCCATTGAGCTTCTGGACCAATTTACAGATGTTGATATGGAACATATACCACGTGAACGTAACTTTGCTGCCAATGAGCTCGCTCAGCTGGCTACAGGCATTAGCTTGAAGTACGGAGTGCGCGAGCGCATCCTGAAGGTTGAGCGTCGCACACTGCCCTTGTGGCTTGCTCGACCTGATCCTCCAGATGATCCCGTGGTTGCGATACTAGAGCCCATTGATGTTGATTGGCGGATTCCACTGATTGAATACCTCAAACAACCAGACTCCAGCGCTGATAGGAAAATTCGTTTCCTCGCATTAAACTACTTCCTTAGAGGCGATGAACTGCGCCGACGTGGGGAAGATGGTATAGATTTTCGATGTGTCTACGGTCGCGAAGCCAAGCGATTGATGCGCGAGGTGCACACGGGGATATGTGGATCTCATCAAGCCGGACCTAAGATGTGTTGGCTCCTCCGACGTCATGGTTATTACTGGCCCAGCATTCTGAAGGATTGTATTGCATTCGCCAAAGGCTGTGCGGACTGTCAAGCGCACGGTCCTGTCCAGCATGTTCCTAACATTCCTATGCAACCTATCATTAAACCTTGGCCTGCGCGAGGCTGGGCATTGGATTTGATTGGGATGATCCATCCTCATTCTTCCCTACAGCACAAGTTTATCATCGTCGCCACTGATTACTTCACGAAATGGGTTGAAGCAGAGCCTCTGAAGGAGGCCTCCGGCGCTACCATTCGCCAGTTTATTTTCCGTAATATTCTCTGCAGGTTCGGTATCCCGGAAGTGCTGGTAACCGGTCGCGGTAAATGGACGCCGCGATGGGAGGGACCCTTTGTTATTCACCGAATATTGGAACGCGGTGCTTTTCACCTCAAAGACATCGACGGCGATATCCATCGCAACCCTATTAACGGTCGTTTTCTGAAGAAATACTATCCCAGTGTCTGGGAGTTTGATGATCCTCCTGACTCTGTGCTTGATCCGACTGGGGGGCAACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

546

Amino Acids

62.69

Weight (kDa)

7.56

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H PF00075 121 - 234 2.8e-06 RNase H
RVT_3 PF13456 124 - 242 2.9e-21 Reverse transcriptase-like
Integrase_H2C2 PF17921 339 - 392 1e-07 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1102
Acc36I ACCTGC 1 cut(s) 1395
AccB7I CCANNNNNTGG 1 cut(s) 1584
AccI GTMKAC 1 cut(s) 1005
AccII CGCG 9 cut(s) 555, 636, 759, 1014, 1035, 1237, 1440, 1456, 1497
AciI CCGC 9 cut(s) 133, 194, 239, 555, 868, 1160, 1440, 1454, 1497
AclWI GGATC 6 cut(s) 809, 821, 1066, 1261, 1592, 1613
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 1 cut(s) 921
AcuI CTGAAG 4 cut(s) 791, 1148, 1370, 1583
AcvI CACGTG 1 cut(s) 689
AcyI GRCGYC 2 cut(s) 1099, 1451
AfaI GTAC 5 cut(s) 183, 255, 472, 604, 749
AfeI AGCGCT 1 cut(s) 910
AfiI CCNNNNNNNGG 8 cut(s) 193, 405, 976, 1238, 1489, 1550, 1584, 1628
AflIII ACRYGT 1 cut(s) 54
AgeI ACCGGT 1 cut(s) 1433
AgsI TTSAA 3 cut(s) 745, 884, 1337
AhdI GACNNNNNGTC 1 cut(s) 515
AjiI CACGTC 1 cut(s) 974
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 7 cut(s) 457, 497, 568, 646, 715, 724, 741
AluI AGCT 7 cut(s) 457, 497, 568, 646, 715, 724, 741
Alw21I GWGCWC 2 cut(s) 717, 1044
Alw44I GTGCAC 1 cut(s) 1040
AlwI GGATC 6 cut(s) 809, 821, 1066, 1261, 1592, 1613
AlwNI CAGNNNCTG 1 cut(s) 1584
Aor51HI AGCGCT 1 cut(s) 910
AoxI GGCC 7 cut(s) 116, 130, 396, 483, 1115, 1229, 1356
ApaLI GTGCAC 1 cut(s) 1040
ApeKI GCWGC 2 cut(s) 613, 704
ApoI RAATTY 1 cut(s) 921
AseI ATTAAT 1 cut(s) 576
AsiGI ACCGGT 1 cut(s) 1433
AspS9I GGNCC 6 cut(s) 535, 652, 1073, 1116, 1178, 1466
AsuC2I CCSGG 3 cut(s) 115, 539, 1418
AsuHPI GGTGA 3 cut(s) 443, 1472, 1499
AvaII GGWCC 5 cut(s) 535, 652, 1073, 1178, 1466
BaeGI GKGCMC 1 cut(s) 1044
BanII GRGCYC 2 cut(s) 717, 852
BbrPI CACGTG 1 cut(s) 689
Bbv12I GWGCWC 2 cut(s) 717, 1044
BbvI GCAGC 2 cut(s) 600, 691
BccI CCATC 6 cut(s) 86, 368, 977, 1278, 1452, 1542
BceAI ACGGC 1 cut(s) 1540
BcgI CGANNNNNNTGC 2 cut(s) 595, 629
BciT130I CCWGG 1 cut(s) 358
BciVI GTATCC 1 cut(s) 1424
BcnI CCSGG 3 cut(s) 115, 539, 1418
BfaI CTAG 3 cut(s) 224, 242, 845
BfmI CTRYAG 4 cut(s) 350, 729, 1286, 1402
BfoI RGCGCY 2 cut(s) 912, 1368
BfuAI ACCTGC 1 cut(s) 1395
BfuI GTATCC 1 cut(s) 1424
BglI GCCNNNNNGGC 1 cut(s) 799
BisI GCNGC 5 cut(s) 133, 556, 614, 705, 1454
BlpI GCTNAGC 1 cut(s) 720
BlsI GCNGC 5 cut(s) 134, 557, 615, 706, 1455
Bme1390I CCNGG 4 cut(s) 115, 358, 539, 1418
Bme18I GGWCC 5 cut(s) 535, 652, 1073, 1178, 1466
BmeRI GACNNNNNGTC 1 cut(s) 515
BmgBI CACGTC 1 cut(s) 974
BmgT120I GGNCC 6 cut(s) 535, 652, 1073, 1116, 1178, 1466
BmiI GGNNCC 4 cut(s) 536, 1091, 1467, 1468
BmrFI CCNGG 4 cut(s) 115, 358, 539, 1418
BmrI ACTGGG 2 cut(s) 1572, 1635
BmsI GCATC 3 cut(s) 318, 774, 1020
BmuI ACTGGG 2 cut(s) 1572, 1635
BpmI CTGGAG 4 cut(s) 158, 437, 804, 889
Bpu1102I GCTNAGC 1 cut(s) 720
BpuEI CTTGAG 2 cut(s) 145, 364
BpuMI CCSGG 3 cut(s) 115, 539, 1418
BsaAI YACGTR 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 1533
BsaHI GRCGYC 2 cut(s) 1099, 1451
BsaJI CCNNGG 4 cut(s) 356, 357, 831, 1226
BsaWI WCCGGW 1 cut(s) 1433
Bsc4I CCNNNNNNNGG 8 cut(s) 193, 405, 976, 1238, 1489, 1550, 1584, 1628
Bse118I RCCGGY 3 cut(s) 398, 428, 1433
Bse1I ACTGG 5 cut(s) 175, 1118, 1378, 1578, 1630
Bse8I GATNNNNATC 1 cut(s) 1533
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 4 cut(s) 356, 357, 831, 1226
BseGI GGATG 6 cut(s) 232, 309, 618, 765, 1269, 1270
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 8 cut(s) 193, 405, 976, 1238, 1489, 1550, 1584, 1628
BseMII CTCAG 3 cut(s) 98, 578, 734
BseNI ACTGG 5 cut(s) 175, 1118, 1378, 1578, 1630
BseRI GAGGAG 1 cut(s) 1082
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 2 cut(s) 600, 691
BseYI CCCAGC 2 cut(s) 1118, 1242
Bsh1236I CGCG 9 cut(s) 555, 636, 759, 1014, 1035, 1237, 1440, 1456, 1497
Bsh1285I CGRYCG 3 cut(s) 1012, 1438, 1555
BshFI GGCC 7 cut(s) 118, 132, 398, 485, 1117, 1231, 1358
BshTI ACCGGT 1 cut(s) 1433
BsiEI CGRYCG 3 cut(s) 1012, 1438, 1555
BsiHKAI GWGCWC 2 cut(s) 717, 1044
BsiSI CCGG 8 cut(s) 115, 399, 429, 538, 1071, 1362, 1418, 1434
BslFI GGGAC 3 cut(s) 97, 554, 1479
BslI CCNNNNNNNGG 8 cut(s) 193, 405, 976, 1238, 1489, 1550, 1584, 1628
BsmFI GGGAC 3 cut(s) 97, 554, 1479
BsmI GAATGC 2 cut(s) 1122, 1142
BsnI GGCC 7 cut(s) 118, 132, 398, 485, 1117, 1231, 1358
Bsp1286I GDGCHC 3 cut(s) 717, 852, 1044
Bsp143I GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
Bsp1720I GCTNAGC 1 cut(s) 720
Bsp68I TCGCGA 1 cut(s) 1014
BspACI CCGC 9 cut(s) 133, 194, 239, 555, 868, 1160, 1440, 1454, 1497
BspANI GGCC 7 cut(s) 118, 132, 398, 485, 1117, 1231, 1358
BspCNI CTCAG 3 cut(s) 97, 577, 733
BspFNI CGCG 9 cut(s) 555, 636, 759, 1014, 1035, 1237, 1440, 1456, 1497
BspLI GGNNCC 4 cut(s) 536, 1091, 1467, 1468
BspMAI CTGCAG 1 cut(s) 1406
BspMI ACCTGC 1 cut(s) 1395
BspPI GGATC 6 cut(s) 809, 821, 1066, 1261, 1592, 1613
BsrFI RCCGGY 3 cut(s) 398, 428, 1433
BsrI ACTGG 5 cut(s) 175, 1118, 1378, 1578, 1630
BssAI RCCGGY 3 cut(s) 398, 428, 1433
BssECI CCNNGG 4 cut(s) 356, 357, 831, 1226
BssMI GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
BssNI GRCGYC 2 cut(s) 1099, 1451
BssT1I CCWWGG 1 cut(s) 1226
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 5 cut(s) 159, 1010, 1166, 1178, 1553
BstACI GRCGYC 2 cut(s) 1099, 1451
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 9 cut(s) 37, 277, 307, 400, 717, 726, 761, 804, 1233
BstDEI CTNAG 5 cut(s) 84, 564, 720, 950, 1077
BstDSI CCRYGG 1 cut(s) 831
BstEII GGTNACC 1 cut(s) 1429
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 6 cut(s) 232, 309, 618, 765, 1269, 1270
BstFNI CGCG 9 cut(s) 555, 636, 759, 1014, 1035, 1237, 1440, 1456, 1497
BstH2I RGCGCY 2 cut(s) 912, 1368
BstKTI GATC 7 cut(s) 436, 817, 829, 1061, 1269, 1600, 1621
BstMBI GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
BstMCI CGRYCG 3 cut(s) 1012, 1438, 1555
BstMWI GCNNNNNNNGC 6 cut(s) 721, 725, 799, 1030, 1039, 1364
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 3 cut(s) 58, 309, 1193
BstPI GGTNACC 1 cut(s) 1429
BstSCI CCNGG 4 cut(s) 113, 356, 537, 1416
BstSFI CTRYAG 4 cut(s) 350, 729, 1286, 1402
BstSLI GKGCMC 1 cut(s) 1044
BstUI CGCG 9 cut(s) 555, 636, 759, 1014, 1035, 1237, 1440, 1456, 1497
BstV1I GCAGC 2 cut(s) 600, 691
BstX2I RGATCY 1 cut(s) 1058
BstYI RGATCY 1 cut(s) 1058
BsuI GTATCC 1 cut(s) 1424
BsuRI GGCC 7 cut(s) 118, 132, 398, 485, 1117, 1231, 1358
BtgI CCRYGG 1 cut(s) 831
BtgZI GCGATG 4 cut(s) 64, 972, 1471, 1520
BtrI CACGTC 1 cut(s) 974
BtsCI GGATG 6 cut(s) 232, 309, 618, 765, 1269, 1270
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 5 cut(s) 155, 788, 875, 1311, 1585
BtuMI TCGCGA 1 cut(s) 1014
BveI ACCTGC 1 cut(s) 1395
Cac8I GCNNGC 9 cut(s) 37, 277, 307, 400, 717, 726, 761, 804, 1233
CaiI CAGNNNCTG 1 cut(s) 1584
Cfr10I RCCGGY 3 cut(s) 398, 428, 1433
Cfr13I GGNCC 6 cut(s) 535, 652, 1073, 1116, 1178, 1466
CseI GACGC 2 cut(s) 770, 1459
Csp6I GTAC 5 cut(s) 182, 254, 471, 603, 748
CspAI ACCGGT 1 cut(s) 1433
CviAII CATG 4 cut(s) 55, 306, 1103, 1190
CviQI GTAC 5 cut(s) 182, 254, 471, 603, 748
DdeI CTNAG 5 cut(s) 84, 564, 720, 950, 1077
DpnI GATC 7 cut(s) 435, 816, 828, 1060, 1268, 1599, 1620
DpnII GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
DriI GACNNNNNGTC 1 cut(s) 515
EaeI YGGCCR 1 cut(s) 396
Eam1105I GACNNNNNGTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 883
Ecl136II GAGCTC 1 cut(s) 715
Eco130I CCWWGG 1 cut(s) 1226
Eco147I AGGCCT 1 cut(s) 1358
Eco24I GRGCYC 2 cut(s) 717, 852
Eco32I GATATC 1 cut(s) 1531
Eco47I GGWCC 5 cut(s) 535, 652, 1073, 1178, 1466
Eco47III AGCGCT 1 cut(s) 910
Eco53kI GAGCTC 1 cut(s) 715
Eco57I CTGAAG 4 cut(s) 791, 1148, 1370, 1583
Eco72I CACGTG 1 cut(s) 689
Eco91I GGTNACC 1 cut(s) 1429
EcoICRI GAGCTC 1 cut(s) 715
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 1466
EcoO65I GGTNACC 1 cut(s) 1429
EcoRII CCWGG 1 cut(s) 356
EcoRV GATATC 1 cut(s) 1531
EcoT14I CCWWGG 1 cut(s) 1226
EcoT22I ATGCAT 1 cut(s) 311
EcoT38I GRGCYC 2 cut(s) 717, 852
ErhI CCWWGG 1 cut(s) 1226
FaeI CATG 4 cut(s) 58, 309, 1106, 1193
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 3 cut(s) 97, 554, 1479
FatI CATG 4 cut(s) 54, 305, 1102, 1189
FblI GTMKAC 1 cut(s) 1005
Fnu4HI GCNGC 5 cut(s) 133, 556, 614, 705, 1454
FokI GGATG 6 cut(s) 219, 296, 605, 752, 1257, 1276
FriOI GRGCYC 2 cut(s) 717, 852
Fsp4HI GCNGC 5 cut(s) 133, 556, 614, 705, 1454
FspBI CTAG 3 cut(s) 224, 242, 845
GluI GCNGC 5 cut(s) 133, 556, 614, 705, 1454
GsaI CCCAGC 2 cut(s) 1122, 1246
GsuI CTGGAG 4 cut(s) 158, 437, 804, 889
HaeII RGCGCY 2 cut(s) 912, 1368
HaeIII GGCC 7 cut(s) 118, 132, 398, 485, 1117, 1231, 1358
HapII CCGG 8 cut(s) 115, 399, 429, 538, 1071, 1362, 1418, 1434
HgaI GACGC 2 cut(s) 770, 1459
Hin1I GRCGYC 2 cut(s) 1099, 1451
Hin1II CATG 4 cut(s) 58, 309, 1106, 1193
HinfI GANTC 8 cut(s) 289, 510, 550, 560, 631, 871, 902, 1607
HpaII CCGG 8 cut(s) 115, 399, 429, 538, 1071, 1362, 1418, 1434
HphI GGTGA 3 cut(s) 443, 1472, 1499
Hpy166II GTNNAC 3 cut(s) 692, 1006, 1042
Hpy188I TCNGA 8 cut(s) 87, 163, 549, 1097, 1128, 1350, 1563, 1623
Hpy8I GTNNAC 3 cut(s) 692, 1006, 1042
Hpy99I CGWCG 5 cut(s) 786, 975, 1101, 1310, 1526
HpyAV CCTTC 4 cut(s) 184, 766, 1123, 1345
HpyCH4III ACNGT 5 cut(s) 159, 1010, 1166, 1178, 1553
HpyCH4IV ACGT 6 cut(s) 461, 543, 688, 694, 973, 1099
HpyCH4V TGCA 8 cut(s) 39, 75, 279, 309, 1042, 1142, 1210, 1404
HpyF10VI GCNNNNNNNGC 6 cut(s) 721, 725, 799, 1030, 1039, 1364
HpyF3I CTNAG 5 cut(s) 84, 564, 720, 950, 1077
HpySE526I ACGT 6 cut(s) 461, 543, 688, 694, 973, 1099
Hsp92I GRCGYC 2 cut(s) 1099, 1451
Hsp92II CATG 4 cut(s) 58, 309, 1106, 1193
KflI GGGWCCC 1 cut(s) 1466
KroI GCCGGC 1 cut(s) 398
KroNI GCCGGC 1 cut(s) 400
Kzo9I GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
LmnI GCTCC 1 cut(s) 1095
Lsp1109I GCAGC 2 cut(s) 600, 691
LweI GCATC 3 cut(s) 318, 774, 1020
MaeI CTAG 3 cut(s) 224, 242, 845
MaeII ACGT 6 cut(s) 461, 543, 688, 694, 973, 1099
MaeIII GTNAC 2 cut(s) 695, 1429
MalI GATC 7 cut(s) 435, 816, 828, 1060, 1268, 1599, 1620
MboI GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
MboII GAAGA 3 cut(s) 992, 1272, 1576
MflI RGATCY 1 cut(s) 1058
MhlI GDGCHC 3 cut(s) 717, 852, 1044
MluCI AATT 3 cut(s) 270, 656, 921
MlyI GAGTC 4 cut(s) 283, 504, 896, 1601
MmeI TCCRAC 3 cut(s) 246, 504, 1120
Mph1103I ATGCAT 1 cut(s) 311
MroNI GCCGGC 1 cut(s) 398
MseI TTAA 6 cut(s) 17, 576, 938, 1221, 1548, 1639
MslI CAYNNNNRTG 1 cut(s) 1205
MspA1I CMGCKG 1 cut(s) 724
MspI CCGG 8 cut(s) 115, 399, 429, 538, 1071, 1362, 1418, 1434
MspR9I CCNGG 4 cut(s) 115, 358, 539, 1418
Mva1269I GAATGC 2 cut(s) 1122, 1142
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 9 cut(s) 555, 636, 759, 1014, 1035, 1237, 1440, 1456, 1497
MwoI GCNNNNNNNGC 6 cut(s) 721, 725, 799, 1030, 1039, 1364
NaeI GCCGGC 1 cut(s) 400
NciI CCSGG 3 cut(s) 115, 539, 1418
NdeII GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
NgoMIV GCCGGC 1 cut(s) 398
NlaIII CATG 4 cut(s) 58, 309, 1106, 1193
NlaIV GGNNCC 4 cut(s) 536, 1091, 1467, 1468
NruI TCGCGA 1 cut(s) 1014
NsiI ATGCAT 1 cut(s) 311
NspI RCATGY 3 cut(s) 58, 309, 1193
PaeI GCATGC 1 cut(s) 309
PasI CCCWGGG 1 cut(s) 357
PceI AGGCCT 1 cut(s) 1358
PciI ACATGT 1 cut(s) 54
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 756
PctI GAATGC 2 cut(s) 1122, 1142
PdiI GCCGGC 1 cut(s) 400
PfeI GAWTC 4 cut(s) 550, 560, 631, 871
PflMI CCANNNNNTGG 1 cut(s) 1584
PfoI TCCNGGA 2 cut(s) 537, 1416
PinAI ACCGGT 1 cut(s) 1433
PkrI GCNGC 5 cut(s) 134, 557, 615, 706, 1455
PleI GAGTC 4 cut(s) 283, 504, 896, 1601
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
PpsI GAGTC 4 cut(s) 283, 504, 896, 1601
Ppu21I YACGTR 1 cut(s) 689
PpuMI RGGWCCY 1 cut(s) 1466
PscI ACATGT 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 717
Psp5II RGGWCCY 1 cut(s) 1466
Psp6I CCWGG 1 cut(s) 356
PspCI CACGTG 1 cut(s) 689
PspEI GGTNACC 1 cut(s) 1429
PspFI CCCAGC 2 cut(s) 1118, 1242
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 4 cut(s) 536, 1091, 1467, 1468
PspPI GGNCC 6 cut(s) 535, 652, 1073, 1116, 1178, 1466
PspPPI RGGWCCY 1 cut(s) 1466
PsrI GAACNNNNNNTAC 2 cut(s) 237, 269
PstI CTGCAG 1 cut(s) 1406
PstNI CAGNNNCTG 1 cut(s) 1584
PsuI RGATCY 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 724
RruI TCGCGA 1 cut(s) 1014
RsaI GTAC 5 cut(s) 183, 255, 472, 604, 749
RsaNI GTAC 5 cut(s) 182, 254, 471, 603, 748
RseI CAYNNNNRTG 1 cut(s) 1205
SacI GAGCTC 1 cut(s) 717
SaqAI TTAA 6 cut(s) 17, 576, 938, 1221, 1548, 1639
SatI GCNGC 5 cut(s) 133, 556, 614, 705, 1454
Sau3AI GATC 7 cut(s) 433, 814, 826, 1058, 1266, 1597, 1618
Sau96I GGNCC 6 cut(s) 535, 652, 1073, 1116, 1178, 1466
SchI GAGTC 4 cut(s) 283, 504, 896, 1601
ScrFI CCNGG 4 cut(s) 115, 358, 539, 1418
SduI GDGCHC 3 cut(s) 717, 852, 1044
SfaNI GCATC 3 cut(s) 318, 774, 1020
SfcI CTRYAG 4 cut(s) 350, 729, 1286, 1402
SinI GGWCC 5 cut(s) 535, 652, 1073, 1178, 1466
SmiMI CAYNNNNRTG 1 cut(s) 1205
SmlI CTYRAG 2 cut(s) 124, 379
SmoI CTYRAG 2 cut(s) 124, 379
SphI GCATGC 1 cut(s) 309
Sse9I AATT 3 cut(s) 270, 656, 921
SseBI AGGCCT 1 cut(s) 1358
SsiI CCGC 9 cut(s) 133, 194, 239, 555, 868, 1160, 1440, 1454, 1497
SspI AATATT 2 cut(s) 1396, 1488
SspMI CTAG 3 cut(s) 224, 242, 845
SstI GAGCTC 1 cut(s) 717
StuI AGGCCT 1 cut(s) 1358
StyD4I CCNGG 4 cut(s) 113, 356, 537, 1416
StyI CCWWGG 1 cut(s) 1226
TaaI ACNGT 5 cut(s) 159, 1010, 1166, 1178, 1553
TaiI ACGT 6 cut(s) 464, 546, 691, 697, 976, 1102
TaqI TCGA 5 cut(s) 292, 513, 808, 997, 1521
TaqII GACCGA 1 cut(s) 104
TasI AATT 3 cut(s) 270, 656, 921
TatI WGTACW 2 cut(s) 253, 470
TauI GCSGC 3 cut(s) 135, 558, 1456
TfiI GAWTC 4 cut(s) 550, 560, 631, 871
Tru1I TTAA 6 cut(s) 17, 576, 938, 1221, 1548, 1639
Tru9I TTAA 6 cut(s) 17, 576, 938, 1221, 1548, 1639
TscAI CASTG 5 cut(s) 162, 795, 882, 1318, 1585
TseI GCWGC 2 cut(s) 613, 704
TspDTI ATGAA 2 cut(s) 507, 975
TspGWI ACGGA 2 cut(s) 765, 1379
TspRI CASTG 5 cut(s) 162, 795, 882, 1318, 1585
Van91I CCANNNNNTGG 1 cut(s) 1584
VneI GTGCAC 1 cut(s) 1040
VpaK11BI GGWCC 5 cut(s) 535, 652, 1073, 1178, 1466
VspI ATTAAT 1 cut(s) 576
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 1 cut(s) 921
XceI RCATGY 3 cut(s) 58, 309, 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 1005
XspI CTAG 3 cut(s) 224, 242, 845
ZraI GACGTC 1 cut(s) 1100
Zsp2I ATGCAT 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.