RchiOBHm_Chr5g0038141

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000160
Physical Location & Seq
Reverse (-)
32761777 .. 32764230
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31678

Sequence Viewer

Length: 2169 bp
ATGTTGGCTCAAGACAATGATCAAGGAAAAGAGCAAGCCATCCATTATTTGCGCAGAATCCTCACACCTGTTGAGATAAGATATTCGCCGATTGAAAAACTATGCCTCGTGCTCTACTTTTCGGTGATTAAATTACGACATTATATGTTACCATCAGTTGTTCATATAATTTCACAAACGAATTTAATTAAATATATGTTGACTCAGCCGATCATCAAAAGCCGAATTGGAAAATGGACGATGGCGCTTTCTGAATTTACTTTCAAATACATGGCTCAGAAGTCAGTCAAAGGCCAGGCATTGGCTCAATTTCTTGCTGACCACCCTTCTGTTGAAATTGAAGATATGGAGAACGTTGAAATAGATACTGCACAAGTCGGCCAGATGTATCACGAGCCTTGGCTACTATATTTTGATGGCTCAAGCACCAGTGACTCCGCCGGGGCAGGAATAGTGATTGAATCTCCAACCGGCCAAAAGCATCAATATGCCTTCAAACTGGACTTTAACTGCACAAACAACAAGGCTGAATATGAAGCCTTAATCATTCGCTTGGAAGTATTGGAAGAGCTTGGAGCAACACGAGTTAAAGTGTTTGGGGACTCACTACTAGTCATCAATCAAATGCTCCAGGTTTTTAGATGTTCAAATCTCTCGTTGGCCACTTATTATGCCGCTGCACAACAACTACTTAGTTGTTTTCATGATGTGGAATTTCACCATCTTCCGCGAGAACTCAATCGTGAAGCGAATGAAATGGCTCAAATTGCTTCCGGCGTCAGCATTCCAGCAGGCCAGACTAACAAAATCATCACCATTGAGAGAAACTCGTTGCCATCTTTGGCCGAGAGGGGTATGCCGGCCGATGTCTTTGAGCTTGACGTGCTACTTGGTAATTGGAGATTTTACATAACCCAACACCTTTTGATGAAAACTGATGGCGGTGGGAGCCGAAAAATTAGAATGTTGTCTAGCAAGTTCACAATTAAAAATGGTGAACTGCTAAGGAAAATTCCTGATGATGACCTTCTTCTTCGTTGCCTCGGCTCCGAAGATGCCCAGCTAGTAATGGCTGAAGTCCATGAGGGTATTTGCGGAGCACACCAGGCTGGGATAAAAATGAGATGGTTAATTAGGAGACATGGCTACTACTGGCCAACTATTCTTAAAGACTGTATTGAGTATGCTCGAGGCTGTGCTCCATGCCAACTTCACGGGTCCATTCAGAGAGCTCCTGCTTTCCCCATGAATCCAATAGTGAAGCCATGGCCTTTTCGGGGCTGGGCAATGGATATAATTGGCCAAATCTCTCCCCCATCTTCCAAACAGCACATATGGATACTGGTGGCCACATACTACTTTACAAAATGGGTTGAAGCCGTTCCATTCACCTCCATAAGTAGTGTCGAGGTGATCAAATTCATTGAGCAAAACATCATTCACTGTTTCGGTATTCCAGAAACTATAACAACTGACCGAGGATCAGTATTCATAGCTGAATCCGTCACCAAACTGATGGCCGAGTATATGATTACAATGCAGCAATCGACACCATATTATGCTCAGGCTAATGGACAGGCTGAGGCCACAAACAAGGTCCTCATCCAAATTGTGGAAAAGATGATTCAGGAAAATCCGCGTGATTGGCACAATCTTCTATCAGAAACTTTATGGGCTTATCAGATCTCCAAACGCTCGGCTACAGGGACAACTCCCTTTGCTCTGACTTATGGACATGATGCAATTTTATCGATGGAAATGACAGTCAGATCATTAAGAGTCGCAATGCAAAACAATCTCACAGCCGACGAATATAGTCAGGCAATGTTGCAAGAACTAGAGGATCTTGACCAGGCGCGGTTGGATGCATATGATCAGCTTCAAGCTCAGAAGAAGGCTGTCGCTCGTGCTTGCAATAAAAAGACCAGATACAAGAGTTTTGGGGAAGGCGAGCTGGTATGGAAAGTAGTGTTACCCATCGGCACGAAGGATCCTAGATTTGGAAAATGGTCACCAAATTGGGAGGGTCCGTTTATTATTGACAAAGTTCTTGGCAAAGGAGCATATCATTTGCGAGATAAAGATGGCGAACGACACGCAATGCCCATTAATAGCCAATACCTAAAGAAATATTATCCCACAATGTGGGAAGCGCGAGCTACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

722

Amino Acids

82.25

Weight (kDa)

7.05

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RT_RNaseH PF17917 1 - 86 5.2e-13 RNase H-like domain found in reverse transcriptase
RT_RNaseH_2 PF17919 1 - 51 5.8e-06 RNase H-like domain found in reverse transcriptase
RVT_3 PF13456 138 - 255 9.9e-18 Reverse transcriptase-like
Integrase_H2C2 PF17921 353 - 405 2.1e-08 Integrase zinc binding domain
rve PF00665 424 - 520 2.6e-10 Integrase core domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 53
AccB7I CCANNNNNTGG 3 cut(s) 301, 1612, 2145
AccII CGCG 4 cut(s) 730, 1639, 1858, 2155
AciI CCGC 7 cut(s) 438, 675, 728, 942, 1095, 1637, 1858
AclI AACGTT 1 cut(s) 354
AclWI GGATC 4 cut(s) 1489, 1851, 1985, 1998
AcoI YGGCCR 9 cut(s) 379, 472, 660, 843, 861, 1154, 1300, 1347, 1518
AcsI RAATTY 5 cut(s) 181, 254, 713, 1011, 1418
AcuI CTGAAG 1 cut(s) 1095
AcyI GRCGYC 1 cut(s) 777
AdeI CACNNNGTG 1 cut(s) 2145
AfiI CCNNNNNNNGG 5 cut(s) 301, 1277, 1612, 2000, 2145
AhlI ACTAGT 1 cut(s) 610
AjiI CACGTC 1 cut(s) 883
AjnI CCWGG 4 cut(s) 294, 630, 1104, 1851
AluBI AGCT 9 cut(s) 571, 877, 1063, 1232, 1496, 1879, 1886, 1954, 2159
AluI AGCT 9 cut(s) 571, 877, 1063, 1232, 1496, 1879, 1886, 1954, 2159
Alw21I GWGCWC 4 cut(s) 114, 1102, 1201, 1234
Alw26I GTCTC 1 cut(s) 1132
AlwI GGATC 4 cut(s) 1489, 1851, 1985, 1998
Ama87I CYCGRG 1 cut(s) 1188
ApeKI GCWGC 2 cut(s) 677, 1540
ApoI RAATTY 5 cut(s) 181, 254, 713, 1011, 1418
AseI ATTAAT 1 cut(s) 2109
Asp700I GAANNNNTTC 1 cut(s) 1380
AspLEI GCGC 4 cut(s) 54, 247, 1858, 2155
AspS9I GGNCC 3 cut(s) 1218, 1597, 2027
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 8 cut(s) 136, 710, 805, 1007, 1381, 1423, 1498, 2004
AvaI CYCGRG 1 cut(s) 1188
AvaII GGWCC 3 cut(s) 1218, 1597, 2027
BaeI ACNNNNGTAYC 2 cut(s) 1921, 1954
BalI TGGCCA 4 cut(s) 662, 1156, 1302, 1349
BamHI GGATCC 1 cut(s) 1990
BanII GRGCYC 1 cut(s) 1234
BauI CACGAG 4 cut(s) 107, 392, 582, 1905
Bbv12I GWGCWC 4 cut(s) 114, 1102, 1201, 1234
BbvCI CCTCAGC 1 cut(s) 1581
BbvI GCAGC 2 cut(s) 664, 1552
BceAI ACGGC 1 cut(s) 1364
BcgI CGANNNNNNTGC 2 cut(s) 1731, 1765
BciT130I CCWGG 4 cut(s) 296, 632, 1106, 1853
BciVI GTATCC 1 cut(s) 1332
BclI TGATCA 3 cut(s) 19, 1413, 1873
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 1132
BcuI ACTAGT 1 cut(s) 610
BfaI CTAG 5 cut(s) 611, 972, 1064, 1838, 1995
BfmI CTRYAG 1 cut(s) 1701
BfoI RGCGCY 1 cut(s) 248
BfuI GTATCC 1 cut(s) 1332
BglII AGATCT 1 cut(s) 1683
BisI GCNGC 3 cut(s) 675, 678, 1541
BlsI GCNGC 3 cut(s) 676, 679, 1542
Bme1390I CCNGG 5 cut(s) 296, 442, 632, 1106, 1853
Bme18I GGWCC 3 cut(s) 1218, 1597, 2027
BmeT110I CYCGRG 1 cut(s) 1188
BmgBI CACGTC 1 cut(s) 883
BmgT120I GGNCC 3 cut(s) 1218, 1597, 2027
BmiI GGNNCC 5 cut(s) 950, 1048, 1219, 1992, 2028
BmrFI CCNGG 5 cut(s) 296, 442, 632, 1106, 1853
BmsI GCATC 4 cut(s) 490, 1045, 1729, 1855
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 3 cut(s) 1004, 1563, 1581
BpuEI CTTGAG 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 1751
BsaHI GRCGYC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 398, 441, 1042, 1265, 1477
BsaXI ACNNNNNCTCC 2 cut(s) 2015, 2045
Bsc4I CCNNNNNNNGG 5 cut(s) 301, 1277, 1612, 2000, 2145
Bse118I RCCGGY 2 cut(s) 470, 859
Bse1I ACTGG 4 cut(s) 429, 504, 1157, 1347
Bse3DI GCAATG 4 cut(s) 1293, 1791, 1830, 2106
BseBI CCWGG 4 cut(s) 296, 632, 1106, 1853
BseCI ATCGAT 1 cut(s) 1751
BseDI CCNNGG 5 cut(s) 398, 441, 1042, 1265, 1477
BseGI GGATG 3 cut(s) 39, 1602, 1870
BseLI CCNNNNNNNGG 5 cut(s) 301, 1277, 1612, 2000, 2145
BseMI GCAATG 4 cut(s) 1293, 1791, 1830, 2106
BseMII CTCAG 5 cut(s) 218, 290, 1572, 1577, 1901
BseNI ACTGG 4 cut(s) 429, 504, 1157, 1347
BseX3I CGGCCG 1 cut(s) 861
BseXI GCAGC 2 cut(s) 664, 1552
BseYI CCCAGC 3 cut(s) 1059, 1109, 1281
BsgI GTGCAG 3 cut(s) 354, 496, 663
Bsh1236I CGCG 4 cut(s) 730, 1639, 1858, 2155
Bsh1285I CGRYCG 1 cut(s) 864
BshVI ATCGAT 1 cut(s) 1751
BsiEI CGRYCG 1 cut(s) 864
BsiHKAI GWGCWC 4 cut(s) 114, 1102, 1201, 1234
BsiHKCI CYCGRG 1 cut(s) 1188
BsiSI CCGG 4 cut(s) 441, 471, 774, 860
BslFI GGGAC 2 cut(s) 614, 1720
BslI CCNNNNNNNGG 5 cut(s) 301, 1277, 1612, 2000, 2145
BsmAI GTCTC 1 cut(s) 1132
BsmFI GGGAC 2 cut(s) 614, 1720
BsmI GAATGC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 1188
Bsp1286I GDGCHC 4 cut(s) 114, 1102, 1201, 1234
Bsp143I GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
Bsp19I CCATGG 1 cut(s) 1265
BspACI CCGC 7 cut(s) 438, 675, 728, 942, 1095, 1637, 1858
BspCNI CTCAG 5 cut(s) 217, 289, 1573, 1576, 1900
BspDI ATCGAT 1 cut(s) 1751
BspFNI CGCG 4 cut(s) 730, 1639, 1858, 2155
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 5 cut(s) 950, 1048, 1219, 1992, 2028
BspPI GGATC 4 cut(s) 1489, 1851, 1985, 1998
BspQI GCTCTTC 1 cut(s) 561
BsrDI GCAATG 4 cut(s) 1293, 1791, 1830, 2106
BsrFI RCCGGY 2 cut(s) 470, 859
BsrI ACTGG 4 cut(s) 429, 504, 1157, 1347
BssAI RCCGGY 2 cut(s) 470, 859
BssECI CCNNGG 5 cut(s) 398, 441, 1042, 1265, 1477
BssMI GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
BssNI GRCGYC 1 cut(s) 777
BssSI CACGAG 4 cut(s) 107, 392, 582, 1905
BssT1I CCWWGG 2 cut(s) 398, 1265
Bst2BI CACGAG 4 cut(s) 107, 392, 582, 1905
Bst2UI CCWGG 4 cut(s) 296, 632, 1106, 1853
Bst4CI ACNGT 3 cut(s) 1175, 1445, 1765
Bst6I CTCTTC 1 cut(s) 561
BstACI GRCGYC 1 cut(s) 777
BstC8I GCNNGC 6 cut(s) 36, 793, 861, 1912, 1952, 2157
BstDEI CTNAG 7 cut(s) 204, 276, 692, 1004, 1563, 1581, 1887
BstDSI CCRYGG 1 cut(s) 1265
BstEII GGTNACC 1 cut(s) 2010
BstF5I GGATG 3 cut(s) 39, 1602, 1870
BstFNI CGCG 4 cut(s) 730, 1639, 1858, 2155
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 4 cut(s) 54, 247, 1858, 2155
BstKTI GATC 9 cut(s) 22, 213, 1416, 1484, 1686, 1772, 1846, 1876, 1993
BstMAI GTCTC 1 cut(s) 1132
BstMBI GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
BstMCI CGRYCG 1 cut(s) 864
BstMWI GCNNNNNNNGC 5 cut(s) 767, 883, 948, 1106, 1645
BstNI CCWGG 4 cut(s) 296, 632, 1106, 1853
BstPI GGTNACC 1 cut(s) 2010
BstSCI CCNGG 5 cut(s) 294, 440, 630, 1104, 1851
BstSFI CTRYAG 1 cut(s) 1701
BstUI CGCG 4 cut(s) 730, 1639, 1858, 2155
BstV1I GCAGC 2 cut(s) 664, 1552
BstX2I RGATCY 3 cut(s) 1683, 1843, 1990
BstXI CCANNNNNNTGG 1 cut(s) 1516
BstYI RGATCY 3 cut(s) 1683, 1843, 1990
BstZI CGGCCG 1 cut(s) 861
Bsu15I ATCGAT 1 cut(s) 1751
BsuI GTATCC 1 cut(s) 1332
BsuTUI ATCGAT 1 cut(s) 1751
BtgI CCRYGG 1 cut(s) 1265
BtrI CACGTC 1 cut(s) 883
BtsCI GGATG 3 cut(s) 39, 1602, 1870
BtsIMutI CAGTG 2 cut(s) 436, 1441
Cac8I GCNNGC 6 cut(s) 36, 793, 861, 1912, 1952, 2157
CciI TCATGA 1 cut(s) 703
CfoI GCGC 4 cut(s) 54, 247, 1858, 2155
Cfr10I RCCGGY 2 cut(s) 470, 859
Cfr13I GGNCC 3 cut(s) 1218, 1597, 2027
ClaI ATCGAT 1 cut(s) 1751
CseI GACGC 1 cut(s) 766
CviAII CATG 8 cut(s) 271, 704, 1082, 1142, 1203, 1246, 1266, 1736
DdeI CTNAG 7 cut(s) 204, 276, 692, 1004, 1563, 1581, 1887
DpnI GATC 9 cut(s) 21, 212, 1415, 1483, 1685, 1771, 1845, 1875, 1992
DpnII GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
DraIII CACNNNGTG 1 cut(s) 2145
EaeI YGGCCR 9 cut(s) 379, 472, 660, 843, 861, 1154, 1300, 1347, 1518
EagI CGGCCG 1 cut(s) 861
Eam1104I CTCTTC 1 cut(s) 561
EarI CTCTTC 1 cut(s) 561
EciI GGCGGA 1 cut(s) 427
Ecl136II GAGCTC 1 cut(s) 1232
EclXI CGGCCG 1 cut(s) 861
Eco130I CCWWGG 2 cut(s) 398, 1265
Eco24I GRGCYC 1 cut(s) 1234
Eco47I GGWCC 3 cut(s) 1218, 1597, 2027
Eco52I CGGCCG 1 cut(s) 861
Eco53kI GAGCTC 1 cut(s) 1232
Eco57I CTGAAG 1 cut(s) 1095
Eco88I CYCGRG 1 cut(s) 1188
Eco91I GGTNACC 1 cut(s) 2010
EcoICRI GAGCTC 1 cut(s) 1232
EcoO109I RGGNCCY 1 cut(s) 1597
EcoO65I GGTNACC 1 cut(s) 2010
EcoRII CCWGG 4 cut(s) 294, 630, 1104, 1851
EcoT14I CCWWGG 2 cut(s) 398, 1265
EcoT22I ATGCAT 1 cut(s) 1870
EcoT38I GRGCYC 1 cut(s) 1234
ErhI CCWWGG 2 cut(s) 398, 1265
FaeI CATG 8 cut(s) 274, 707, 1085, 1145, 1206, 1249, 1269, 1739
FaqI GGGAC 2 cut(s) 614, 1720
FatI CATG 8 cut(s) 270, 703, 1081, 1141, 1202, 1245, 1265, 1735
FauNDI CATATG 2 cut(s) 1334, 1870
FbaI TGATCA 3 cut(s) 19, 1413, 1873
Fnu4HI GCNGC 3 cut(s) 675, 678, 1541
FokI GGATG 3 cut(s) 26, 1589, 1877
FriOI GRGCYC 1 cut(s) 1234
Fsp4HI GCNGC 3 cut(s) 675, 678, 1541
FspBI CTAG 5 cut(s) 611, 972, 1064, 1838, 1995
FspI TGCGCA 1 cut(s) 53
GlaI GCGC 4 cut(s) 53, 246, 1857, 2154
GluI GCNGC 3 cut(s) 675, 678, 1541
GsaI CCCAGC 3 cut(s) 1063, 1113, 1285
GsuI CTGGAG 1 cut(s) 614
HaeII RGCGCY 1 cut(s) 248
HapII CCGG 4 cut(s) 441, 471, 774, 860
HgaI GACGC 1 cut(s) 766
HhaI GCGC 4 cut(s) 54, 247, 1858, 2155
Hin1I GRCGYC 1 cut(s) 777
Hin1II CATG 8 cut(s) 274, 707, 1085, 1145, 1206, 1249, 1269, 1739
Hin6I GCGC 4 cut(s) 52, 245, 1856, 2153
HinP1I GCGC 4 cut(s) 52, 245, 1856, 2153
HincII GTYRAC 1 cut(s) 201
HindII GTYRAC 1 cut(s) 201
HinfI GANTC 9 cut(s) 57, 202, 434, 461, 602, 1249, 1499, 1624, 1779
HpaII CCGG 4 cut(s) 441, 471, 774, 860
HphI GGTGA 8 cut(s) 136, 710, 805, 1007, 1381, 1423, 1498, 2004
Hpy166II GTNNAC 3 cut(s) 201, 981, 998
Hpy188I TCNGA 9 cut(s) 253, 279, 1051, 1227, 1663, 1683, 1725, 1769, 1890
Hpy188III TCNNGA 8 cut(s) 11, 392, 704, 743, 1016, 1457, 1628, 1847
Hpy8I GTNNAC 3 cut(s) 201, 981, 998
Hpy99I CGWCG 1 cut(s) 1811
HpyAV CCTTC 6 cut(s) 336, 502, 1037, 1888, 1940, 1981
HpyCH4III ACNGT 3 cut(s) 1175, 1445, 1765
HpyCH4IV ACGT 2 cut(s) 354, 882
HpyCH4V TGCA 9 cut(s) 371, 513, 680, 1540, 1742, 1789, 1831, 1868, 1914
HpyF10VI GCNNNNNNNGC 5 cut(s) 767, 883, 948, 1106, 1645
HpyF3I CTNAG 7 cut(s) 204, 276, 692, 1004, 1563, 1581, 1887
HpySE526I ACGT 2 cut(s) 354, 882
Hsp92I GRCGYC 1 cut(s) 777
Hsp92II CATG 8 cut(s) 274, 707, 1085, 1145, 1206, 1249, 1269, 1739
HspAI GCGC 4 cut(s) 52, 245, 1856, 2153
KroI GCCGGC 1 cut(s) 859
KroNI GCCGGC 1 cut(s) 861
Ksp22I TGATCA 3 cut(s) 19, 1413, 1873
Kzo9I GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 8 cut(s) 575, 633, 948, 1052, 1097, 1204, 1237, 2060
Lsp1109I GCAGC 2 cut(s) 664, 1552
LweI GCATC 4 cut(s) 490, 1045, 1729, 1855
MaeI CTAG 5 cut(s) 611, 972, 1064, 1838, 1995
MaeII ACGT 2 cut(s) 354, 882
MaeIII GTNAC 5 cut(s) 147, 431, 1504, 1971, 2010
MalI GATC 9 cut(s) 21, 212, 1415, 1483, 1685, 1771, 1845, 1875, 1992
MboI GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
MboII GAAGA 9 cut(s) 353, 578, 716, 1022, 1025, 1064, 1311, 1646, 1903
MflI RGATCY 3 cut(s) 1683, 1843, 1990
MhlI GDGCHC 4 cut(s) 114, 1102, 1201, 1234
MlsI TGGCCA 4 cut(s) 662, 1156, 1302, 1349
MluNI TGGCCA 4 cut(s) 662, 1156, 1302, 1349
MlyI GAGTC 4 cut(s) 196, 428, 596, 1788
MmeI TCCRAC 2 cut(s) 491, 1842
Mox20I TGGCCA 4 cut(s) 662, 1156, 1302, 1349
Mph1103I ATGCAT 1 cut(s) 1870
MroNI GCCGGC 1 cut(s) 859
MroXI GAANNNNTTC 1 cut(s) 1380
MscI TGGCCA 4 cut(s) 662, 1156, 1302, 1349
MslI CAYNNNNRTG 1 cut(s) 486
Msp20I TGGCCA 4 cut(s) 662, 1156, 1302, 1349
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 4 cut(s) 441, 471, 774, 860
MspR9I CCNGG 5 cut(s) 296, 442, 632, 1106, 1853
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 4 cut(s) 296, 632, 1106, 1853
MvnI CGCG 4 cut(s) 730, 1639, 1858, 2155
MwoI GCNNNNNNNGC 5 cut(s) 767, 883, 948, 1106, 1645
NaeI GCCGGC 1 cut(s) 861
NciI CCSGG 1 cut(s) 442
NcoI CCATGG 1 cut(s) 1265
NdeI CATATG 2 cut(s) 1334, 1870
NdeII GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
NgoMIV GCCGGC 1 cut(s) 859
NlaIII CATG 8 cut(s) 274, 707, 1085, 1145, 1206, 1249, 1269, 1739
NlaIV GGNNCC 5 cut(s) 950, 1048, 1219, 1992, 2028
NmeAIII GCCGAG 4 cut(s) 871, 1023, 1546, 1676
NmuCI GTSAC 3 cut(s) 431, 1504, 2010
NsbI TGCGCA 1 cut(s) 53
NsiI ATGCAT 1 cut(s) 1870
PacI TTAATTAA 1 cut(s) 189
PaeR7I CTCGAG 1 cut(s) 1188
PagI TCATGA 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 561
PctI GAATGC 1 cut(s) 783
PdiI GCCGGC 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 1380
PfeI GAWTC 5 cut(s) 57, 461, 1249, 1499, 1624
PflMI CCANNNNNTGG 3 cut(s) 301, 1612, 2145
PkrI GCNGC 3 cut(s) 676, 679, 1542
PleI GAGTC 4 cut(s) 196, 428, 596, 1787
PpsI GAGTC 4 cut(s) 196, 428, 596, 1787
PpuMI RGGWCCY 1 cut(s) 1597
PshBI ATTAAT 1 cut(s) 2109
Psp124BI GAGCTC 1 cut(s) 1234
Psp1406I AACGTT 1 cut(s) 354
Psp5II RGGWCCY 1 cut(s) 1597
Psp6I CCWGG 4 cut(s) 294, 630, 1104, 1851
PspEI GGTNACC 1 cut(s) 2010
PspFI CCCAGC 3 cut(s) 1059, 1109, 1281
PspGI CCWGG 4 cut(s) 294, 630, 1104, 1851
PspN4I GGNNCC 5 cut(s) 950, 1048, 1219, 1992, 2028
PspPI GGNCC 3 cut(s) 1218, 1597, 2027
PspPPI RGGWCCY 1 cut(s) 1597
PspXI VCTCGAGB 1 cut(s) 1188
PsuI RGATCY 3 cut(s) 1683, 1843, 1990
RseI CAYNNNNRTG 1 cut(s) 486
SacI GAGCTC 1 cut(s) 1234
SapI GCTCTTC 1 cut(s) 561
SatI GCNGC 3 cut(s) 675, 678, 1541
Sau3AI GATC 9 cut(s) 19, 210, 1413, 1481, 1683, 1769, 1843, 1873, 1990
Sau96I GGNCC 3 cut(s) 1218, 1597, 2027
SchI GAGTC 4 cut(s) 196, 428, 596, 1788
ScrFI CCNGG 5 cut(s) 296, 442, 632, 1106, 1853
SduI GDGCHC 4 cut(s) 114, 1102, 1201, 1234
SfaNI GCATC 4 cut(s) 490, 1045, 1729, 1855
SfcI CTRYAG 1 cut(s) 1701
Sfr274I CTCGAG 1 cut(s) 1188
SinI GGWCC 3 cut(s) 1218, 1597, 2027
SlaI CTCGAG 1 cut(s) 1188
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 3 cut(s) 9, 421, 1188
SmoI CTYRAG 3 cut(s) 9, 421, 1188
SpeI ACTAGT 1 cut(s) 610
SsiI CCGC 7 cut(s) 438, 675, 728, 942, 1095, 1637, 1858
SspI AATATT 1 cut(s) 2132
SspMI CTAG 5 cut(s) 611, 972, 1064, 1838, 1995
SstI GAGCTC 1 cut(s) 1234
StyD4I CCNGG 5 cut(s) 294, 440, 630, 1104, 1851
StyI CCWWGG 2 cut(s) 398, 1265
TaaI ACNGT 3 cut(s) 1175, 1445, 1765
TaiI ACGT 2 cut(s) 357, 885
TaqI TCGA 4 cut(s) 1189, 1407, 1547, 1751
TaqII GACCGA 1 cut(s) 1491
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 5 cut(s) 57, 461, 1249, 1499, 1624
TscAI CASTG 2 cut(s) 436, 1448
TseFI GTSAC 3 cut(s) 431, 1504, 2010
TseI GCWGC 2 cut(s) 677, 1540
Tsp45I GTSAC 3 cut(s) 431, 1504, 2010
TspDTI ATGAA 8 cut(s) 152, 549, 692, 768, 944, 1262, 1411, 1480
TspGWI ACGGA 2 cut(s) 1492, 2019
TspRI CASTG 2 cut(s) 436, 1448
Van91I CCANNNNNTGG 3 cut(s) 301, 1612, 2145
VpaK11BI GGWCC 3 cut(s) 1218, 1597, 2027
VspI ATTAAT 1 cut(s) 2109
XapI RAATTY 5 cut(s) 181, 254, 713, 1011, 1418
XhoI CTCGAG 1 cut(s) 1188
XmnI GAANNNNTTC 1 cut(s) 1380
XspI CTAG 5 cut(s) 611, 972, 1064, 1838, 1995
Zsp2I ATGCAT 1 cut(s) 1870
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.