Rw7G013050

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000160
Physical Location & Seq
Reverse (-)
12379733 .. 12383251
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G013050.1

Sequence Viewer

Length: 2922 bp
ATGGATTACGACCTCACTGAAGAGGGAATAGAAGCCTTCCACTCGATACATGAACGATTGTCATCATACATAGTGGAAAAAGAGGCTTACGATCGCATTGCGACCTTAGAGGTCGTTAATGAAGAACTTGCTGACCCGGAGGACGAAGAGGAAGTCCATATTCAGCTCGCGCCTGCGGCGCTGGACGATACACCTCCCAAGGTTAAAGACCCTACTGAGAAGGTCAACCTGGGCACTGAGAGCGAGCCTATGGAAGTGTCCATCAGTGCTTACCTAGAGTCTGATGAGAAACAGAGGCTCATCGAGCTATTGCTAGAGTTCAAAGATTGTTTCGCGGAGAAGTATGAGGACATGCCCGGCCTGTCACCAGACTTGGTTTGTCATCAATTACCAACCCTCGCTGACAGGAGGCCGGTTAAACAGGAGCCTAGACGTATGAACTCAGAAACCCAGATTCTGGTAAAGGTAGAGGTAGAGAAAATGCACAAGTCAGGCATCATAAGGGTTGCCAAGTATAACCAGTGGCTATCTAATATAGTGCCTGTCCGGAAGAAAAATGGCAAGATGAGAGTCTGCGTTGATTATAGAGACTTGAACCTCGCCACACCTAAAGATGTTTACCCCATGCCGGTCGCGGATATGTTGGTGGACGCGGTGGCAGGCCATGAACTGTTGTCCTTCATGGACGGCACTGCAGGCTATCACCAGATTCCGGTCGCTGAAGAAGACAGACACAAGACTGCTTTCCGTTGCCCTGGCTTCGCGGGAGTTTTCGAATATGTGGTCATGCCTTTTGGACTGAAGAACGCCGGTGCAACGTATCAAAGAGCCATGAACTTGATCTTCCACGACATACTGGGAAAGGTTTTAGAGATATACATTGATGATGTAGTCGTCAAATCTCAGAAGCGAGGGGATCACATCGCGGATCTCAGGAAAGTGTTTGAGCGCATGCGCAGACATAGACTCAAGATGAACCCGGCCAAGTGCGTGTTTGGGGTTCAAGCAGGGGACTTTCTGGGGTTTGTAGTCCACCAGCGAGGAATTGAGGTCCCTGAGGACAAGGCAAGCGCAGTCATTAATGCATCTCCCCCACGGACGAAGAAGGAATTACAGCGTCTTTTGGATTTTCTAGCGCATCATCCTCCGCTAGAGCTACCCGTACTGAAAGAGTTGGAAATTGCTTGCAACACTGCGACTCGACCAGATAAAACATGCATCCCGGAATACGCGGTTTGGTATCAGGCTACAATCTCTTTACAGCCCTGGGTGTTATTCTTTGATGGCTCAAGAACGGACGCTATGGCTGGCGCGGGGATTGTGTTGGAAAACCCGGCGGGTGATCGTTTCTCTTATTCTTTTCAGCTCACGTTCCAATGCACTAATAATCAGGCTGAGTATGAAGCTCTTATCATTGGACTCGAAGTCTTGCTGGAAATGGGCGTCCGGGACGTTCAGATTCGCGGAGATTCTCAGCTCGTTATCAATCAACTTCTGGAAAAATATCGCTGTACGAGTTGGCTGCTCATCCCATACTTGAATCGTGCCATTGAGCTTTTGGACCAGTTTACAGACGTCGACATGGAACACATACCACGTGAGCGTAACTTTGCTGCTAATGAGCTAGCTCAGTTGGCTACAGGCATTACTTTGAAGTATGGAGTGCGCGAGCGCATTCTGAAAGTTGAGCGTCGCACGCTACCTTCGTGGCTGGCTCGACCTGATCCTCCAGATGACCCCATGGTCGCGGTACTGGAACCCATTGATGTCGATTGGCGGATCCCACTGATTGAATACCTCAAACAACCAGACTCCAGCGCTGATAGGAAGATTCGTTTCCTCGCATTGAATTACTTCCTTAGAGGCGATGAACTGCGTAGACGTGGAGAAGATGGTATAGATTTTCGGTGTGTCTACGGTCGCGAAGCCAAGAGATTGATGCGTGAGGTACACACGGGGATATGTGGTTCCCATCAGGCTGGACCTAAGATGCGTTGGCTCCTCCGACGTCATGGTTATTATTGGCCCAGCATTTTGAAGGATTGTATTGCATTCGCCAAAGGCTGTGCGGACTGTCAAGCACACGGTCCTGTCCAGCATGTTCCTAACATTCCTATGCAACCCATCATTAAACCCTGGCCTGCACGAGGCTGGGCATTGGACTTGATTGGGATGATCCATCCTCATTCGTCCCTACAGCACAAGTTTATCATCGTTGCTACAGATTACTTCACGAAGTGGGTTGAAGCAGAGCCTCTCAAGGAGGCCTCCGGTGCTACCATTCGCCAGGGGGCAGCGTTCATGGGAGGCCCCGTTGAGGAGCTGGCGAATGACTTCGGTATTCAATTCATACATAGTACTCCGTACTATCCTCAATCTAATGGTCAGGCGGAGGCGAGCAACAAGACAATCATCACCTTATTGAAGAAGATGTTGGTGGAGAATCCTCGACAGTGGCATGATACTTTGTACGAGACACTTTGGGCCTATCGTACTTCTAAACGTAACCCTACCGCCACGACGCCATGCGCGCTTATGTTTGGACATGACGCTGTCTTACCGTTAGAAATTAATGTTCATTCCCTACGAGTCCAAGAGCAGCATCATTTGATTGGCGAGGATTATGTTCAAGCAATGTGGCAGGAGCACGAAGATTTAAGCGAGCAGCGCTTGGCTGCTTTGGACAATTTGGTGATGGAGAAACAGCGTATCGCCCGCGCCTATGATAGGCGGACGAGGGGTCGCAGTTTCAAAGAGGATGACCTTGTTTGGAAGGCCATTTTACCCTTAGGTGAAAAGGTAACCGGTCGTGGAAAATGGACGCCGCGATGGGAGGGGCCCTTTGTTATCCACCGCATATTGGAGCGCGGCGCTTTTCACCTCAAAGACATCGACGGTGATATCCATCGCAACCCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

973

Amino Acids

111.38

Weight (kDa)

6.01

Isoelectric Point (pI)

41.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 182 - 343 1.5e-31 Reverse transcriptase (RNA-dependent DNA polymerase)
RNase_H PF00075 424 - 537 5.6e-06 RNase H
RVT_3 PF13456 427 - 545 2.1e-20 Reverse transcriptase-like
Integrase_H2C2 PF17921 642 - 695 2.3e-07 Integrase zinc binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1742
AatII GACGTC 2 cut(s) 1578, 2011
Acc16I TGCGCA 1 cut(s) 956
AccB7I CCANNNNNTGG 1 cut(s) 457
AccI GTMKAC 3 cut(s) 1578, 1879, 1914
AccIII TCCGGA 1 cut(s) 546
AclWI GGATC 6 cut(s) 924, 936, 1718, 1774, 1787, 2170
AcoI YGGCCR 1 cut(s) 981
AcuI CTGAAG 3 cut(s) 39, 741, 821
AcvI CACGTG 1 cut(s) 1598
AcyI GRCGYC 5 cut(s) 1443, 1575, 2008, 2522, 2822
AdeI CACNNNGTG 1 cut(s) 2238
AfaI GTAC 8 cut(s) 1164, 1513, 1752, 1950, 2359, 2366, 2471, 2494
AfeI AGCGCT 2 cut(s) 1819, 2669
AfiI CCNNNNNNNGG 7 cut(s) 457, 628, 712, 2147, 2317, 2727, 2860
AgeI ACCGGT 1 cut(s) 2804
AhdI GACNNNNNGTC 2 cut(s) 1424, 2739
AjiI CACGTC 1 cut(s) 1883
AjnI CCWGG 5 cut(s) 228, 754, 1265, 2135, 2286
AjuI GAANNNNNNNTTGG 2 cut(s) 2417, 2449
AloI GAACNNNNNNTCC 2 cut(s) 1951, 1983
Alw21I GWGCWC 1 cut(s) 2649
Alw26I GTCTC 2 cut(s) 582, 2468
AlwI GGATC 6 cut(s) 924, 936, 1718, 1774, 1787, 2170
AlwNI CAGNNNCTG 1 cut(s) 457
Aor13HI TCCGGA 1 cut(s) 546
Aor51HI AGCGCT 2 cut(s) 1819, 2669
ApaI GGGCCC 1 cut(s) 2841
ApeKI GCWGC 6 cut(s) 1522, 1613, 2294, 2599, 2665, 2675
ArsI GACNNNNNNTTYG 4 cut(s) 138, 170, 768, 800
AseI ATTAAT 2 cut(s) 1080, 2571
AsiGI ACCGGT 1 cut(s) 2804
Asp700I GAANNNNTTC 2 cut(s) 1853, 2333
AspS9I GGNCC 9 cut(s) 1051, 1561, 1982, 2025, 2087, 2309, 2484, 2837, 2838
AsuC2I CCSGG 6 cut(s) 137, 357, 980, 1223, 1334, 1448
AsuHPI GGTGA 8 cut(s) 357, 695, 1352, 2407, 2704, 2804, 2870, 2909
AsuII TTCGAA 1 cut(s) 774
AsuNHI GCTAGC 1 cut(s) 1624
AvaII GGWCC 4 cut(s) 1051, 1561, 1982, 2087
AxyI CCTNAGG 2 cut(s) 1056, 2788
BaeGI GKGCMC 2 cut(s) 236, 2841
BamHI GGATCC 1 cut(s) 1779
BanII GRGCYC 1 cut(s) 2841
BauI CACGAG 1 cut(s) 2145
BbrPI CACGTG 1 cut(s) 1598
BbsI GAAGAC 1 cut(s) 732
Bbv12I GWGCWC 1 cut(s) 2649
BbvI GCAGC 6 cut(s) 1509, 1600, 2306, 2611, 2662, 2677
BccI CCATC 9 cut(s) 269, 1277, 1886, 1980, 2132, 2187, 2689, 2823, 2913
BceAI ACGGC 1 cut(s) 703
BcgI CGANNNNNNTGC 4 cut(s) 80, 114, 1504, 1538
BciT130I CCWGG 5 cut(s) 230, 756, 1267, 2137, 2288
BcnI CCSGG 6 cut(s) 137, 357, 980, 1223, 1334, 1448
BcoDI GTCTC 2 cut(s) 582, 2468
BfaI CTAG 6 cut(s) 275, 314, 429, 1133, 1151, 1625
BfmI CTRYAG 4 cut(s) 693, 1638, 2195, 2220
BfoI RGCGCY 4 cut(s) 182, 1821, 2671, 2874
BisI GCNGC 9 cut(s) 177, 1523, 1614, 2295, 2600, 2666, 2676, 2825, 2869
BlsI GCNGC 9 cut(s) 178, 1524, 1615, 2296, 2601, 2667, 2677, 2826, 2870
BmcAI AGTACT 1 cut(s) 2359
Bme18I GGWCC 4 cut(s) 1051, 1561, 1982, 2087
BmeRI GACNNNNNGTC 2 cut(s) 1424, 2739
BmgBI CACGTC 1 cut(s) 1883
BmgT120I GGNCC 9 cut(s) 1051, 1561, 1982, 2025, 2087, 2309, 2484, 2837, 2838
BmiI GGNNCC 9 cut(s) 426, 1053, 1758, 1781, 1969, 2000, 2311, 2838, 2839
BmrI ACTGGG 1 cut(s) 866
BmsI GCATC 7 cut(s) 504, 1094, 1147, 1227, 1929, 1980, 2611
BmtI GCTAGC 1 cut(s) 1628
BmuI ACTGGG 1 cut(s) 866
BpiI GAAGAC 1 cut(s) 732
BpmI CTGGAG 2 cut(s) 1713, 1798
Bpu14I TTCGAA 1 cut(s) 774
BpuEI CTTGAG 3 cut(s) 953, 1273, 2243
BpuMI CCSGG 6 cut(s) 137, 357, 980, 1223, 1334, 1448
BsaAI YACGTR 1 cut(s) 1598
BsaBI GATNNNNATC 2 cut(s) 61, 2904
BsaHI GRCGYC 5 cut(s) 1443, 1575, 2008, 2522, 2822
BsaJI CCNNGG 9 cut(s) 198, 229, 754, 1094, 1265, 1266, 1740, 2135, 2287
BsaWI WCCGGW 4 cut(s) 546, 712, 2270, 2804
BsaXI ACNNNNNCTCC 2 cut(s) 2298, 2328
Bsc4I CCNNNNNNNGG 7 cut(s) 457, 628, 712, 2147, 2317, 2727, 2860
Bse118I RCCGGY 4 cut(s) 412, 628, 809, 2804
Bse1I ACTGG 4 cut(s) 520, 861, 1564, 1758
Bse21I CCTNAGG 2 cut(s) 1056, 2788
Bse3DI GCAATG 2 cut(s) 96, 2640
Bse8I GATNNNNATC 2 cut(s) 61, 2904
BseAI TCCGGA 1 cut(s) 546
BseBI CCWGG 5 cut(s) 230, 756, 1267, 2137, 2288
BseDI CCNNGG 9 cut(s) 198, 229, 754, 1094, 1265, 1266, 1740, 2135, 2287
BseGI GGATG 6 cut(s) 1141, 1218, 1527, 2178, 2179, 2764
BseJI GATNNNNATC 2 cut(s) 61, 2904
BseLI CCNNNNNNNGG 7 cut(s) 457, 628, 712, 2147, 2317, 2727, 2860
BseMI GCAATG 2 cut(s) 96, 2640
BseMII CTCAG 9 cut(s) 207, 228, 456, 917, 946, 1047, 1386, 1487, 1643
BseNI ACTGG 4 cut(s) 520, 861, 1564, 1758
BsePI GCGCGC 1 cut(s) 2529
BseRI GAGGAG 2 cut(s) 1991, 2333
BseSI GKGCMC 2 cut(s) 236, 2841
BseXI GCAGC 6 cut(s) 1509, 1600, 2306, 2611, 2662, 2677
BseYI CCCAGC 2 cut(s) 2027, 2151
BsgI GTGCAG 1 cut(s) 2127
Bsh1285I CGRYCG 5 cut(s) 94, 633, 717, 1921, 2809
BshTI ACCGGT 1 cut(s) 2804
BsiEI CGRYCG 5 cut(s) 94, 633, 717, 1921, 2809
BsiHKAI GWGCWC 1 cut(s) 2649
BslFI GGGAC 4 cut(s) 1025, 1037, 1463, 2176
BslI CCNNNNNNNGG 7 cut(s) 457, 628, 712, 2147, 2317, 2727, 2860
BsmAI GTCTC 2 cut(s) 582, 2468
BsmFI GGGAC 4 cut(s) 1025, 1037, 1463, 2176
BsmI GAATGC 2 cut(s) 1674, 2051
Bsp119I TTCGAA 1 cut(s) 774
Bsp120I GGGCCC 1 cut(s) 2837
Bsp1286I GDGCHC 3 cut(s) 236, 2649, 2841
Bsp13I TCCGGA 1 cut(s) 546
Bsp143I GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
Bsp19I CCATGG 1 cut(s) 1740
Bsp68I TCGCGA 1 cut(s) 1923
BspCNI CTCAG 9 cut(s) 208, 229, 455, 916, 945, 1048, 1387, 1486, 1642
BspEI TCCGGA 1 cut(s) 546
BspLI GGNNCC 9 cut(s) 426, 1053, 1758, 1781, 1969, 2000, 2311, 2838, 2839
BspMAI CTGCAG 1 cut(s) 697
BspOI GCTAGC 1 cut(s) 1628
BspPI GGATC 6 cut(s) 924, 936, 1718, 1774, 1787, 2170
BspT104I TTCGAA 1 cut(s) 774
BsrDI GCAATG 2 cut(s) 96, 2640
BsrFI RCCGGY 4 cut(s) 412, 628, 809, 2804
BsrI ACTGG 4 cut(s) 520, 861, 1564, 1758
BssAI RCCGGY 4 cut(s) 412, 628, 809, 2804
BssECI CCNNGG 9 cut(s) 198, 229, 754, 1094, 1265, 1266, 1740, 2135, 2287
BssHII GCGCGC 1 cut(s) 2529
BssMI GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
BssNI GRCGYC 5 cut(s) 1443, 1575, 2008, 2522, 2822
BssSI CACGAG 1 cut(s) 2145
BssT1I CCWWGG 2 cut(s) 198, 1740
Bst2BI CACGAG 1 cut(s) 2145
Bst2UI CCWGG 5 cut(s) 230, 756, 1267, 2137, 2288
Bst4CI ACNGT 7 cut(s) 672, 1919, 2075, 2087, 2454, 2562, 2897
Bst6I CTCTTC 2 cut(s) 15, 141
BstACI GRCGYC 5 cut(s) 1443, 1575, 2008, 2522, 2822
BstBAI YACGTR 1 cut(s) 1598
BstBI TTCGAA 1 cut(s) 774
BstDSI CCRYGG 2 cut(s) 1094, 1740
BstEII GGTNACC 1 cut(s) 2800
BstENI CCTNNNNNAGG 2 cut(s) 2145, 2725
BstF5I GGATG 6 cut(s) 1141, 1218, 1527, 2178, 2179, 2764
BstH2I RGCGCY 4 cut(s) 182, 1821, 2671, 2874
BstKTI GATC 8 cut(s) 94, 843, 919, 931, 1345, 1726, 1782, 2178
BstMAI GTCTC 2 cut(s) 582, 2468
BstMBI GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
BstMCI CGRYCG 5 cut(s) 94, 633, 717, 1921, 2809
BstNI CCWGG 5 cut(s) 230, 756, 1267, 2137, 2288
BstNSI RCATGY 4 cut(s) 355, 955, 1218, 2102
BstPI GGTNACC 1 cut(s) 2800
BstSFI CTRYAG 4 cut(s) 693, 1638, 2195, 2220
BstSLI GKGCMC 2 cut(s) 236, 2841
BstV1I GCAGC 6 cut(s) 1509, 1600, 2306, 2611, 2662, 2677
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 2 cut(s) 928, 1779
BstXI CCANNNNNNTGG 1 cut(s) 1979
BstYI RGATCY 2 cut(s) 928, 1779
Bsu36I CCTNAGG 2 cut(s) 1056, 2788
BtgI CCRYGG 2 cut(s) 1094, 1740
BtgZI GCGATG 4 cut(s) 907, 1881, 2842, 2891
BtrI CACGTC 1 cut(s) 1883
BtsCI GGATG 6 cut(s) 1141, 1218, 1527, 2178, 2179, 2764
BtsI GCAGTG 2 cut(s) 690, 1191
BtsIMutI CAGTG 8 cut(s) 15, 234, 271, 527, 690, 1191, 1784, 2459
BtuMI TCGCGA 1 cut(s) 1923
CaiI CAGNNNCTG 1 cut(s) 457
Cfr10I RCCGGY 4 cut(s) 412, 628, 809, 2804
Cfr13I GGNCC 9 cut(s) 1051, 1561, 1982, 2025, 2087, 2309, 2484, 2837, 2838
CseI GACGC 8 cut(s) 659, 1106, 1307, 1432, 1679, 2530, 2558, 2830
Csp6I GTAC 8 cut(s) 1163, 1512, 1751, 1949, 2358, 2365, 2470, 2493
CspAI ACCGGT 1 cut(s) 2804
CspCI CAANNNNNGTGG 2 cut(s) 2618, 2653
CviQI GTAC 8 cut(s) 1163, 1512, 1751, 1949, 2358, 2365, 2470, 2493
DpnI GATC 8 cut(s) 93, 842, 918, 930, 1344, 1725, 1781, 2177
DpnII GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
DraIII CACNNNGTG 1 cut(s) 2238
DrdI GACNNNNNNGTC 1 cut(s) 1742
DriI GACNNNNNGTC 2 cut(s) 1424, 2739
DseDI GACNNNNNNGTC 1 cut(s) 1742
EaeI YGGCCR 1 cut(s) 981
Eam1104I CTCTTC 2 cut(s) 15, 141
Eam1105I GACNNNNNGTC 2 cut(s) 1424, 2739
EarI CTCTTC 2 cut(s) 15, 141
EciI GGCGGA 3 cut(s) 1792, 2405, 2746
Eco130I CCWWGG 2 cut(s) 198, 1740
Eco147I AGGCCT 1 cut(s) 2267
Eco24I GRGCYC 1 cut(s) 2841
Eco32I GATATC 1 cut(s) 2902
Eco47I GGWCC 4 cut(s) 1051, 1561, 1982, 2087
Eco47III AGCGCT 2 cut(s) 1819, 2669
Eco57I CTGAAG 3 cut(s) 39, 741, 821
Eco72I CACGTG 1 cut(s) 1598
Eco81I CCTNAGG 2 cut(s) 1056, 2788
Eco91I GGTNACC 1 cut(s) 2800
EcoNI CCTNNNNNAGG 2 cut(s) 2145, 2725
EcoO109I RGGNCCY 4 cut(s) 1051, 2309, 2837, 2838
EcoO65I GGTNACC 1 cut(s) 2800
EcoRII CCWGG 5 cut(s) 228, 754, 1265, 2135, 2286
EcoRV GATATC 1 cut(s) 2902
EcoT14I CCWWGG 2 cut(s) 198, 1740
EcoT22I ATGCAT 2 cut(s) 1087, 1220
EcoT38I GRGCYC 1 cut(s) 2841
ErhI CCWWGG 2 cut(s) 198, 1740
FaqI GGGAC 4 cut(s) 1025, 1037, 1463, 2176
FauI CCCGC 4 cut(s) 757, 1306, 1330, 2723
FblI GTMKAC 3 cut(s) 1578, 1879, 1914
Fnu4HI GCNGC 9 cut(s) 177, 1523, 1614, 2295, 2600, 2666, 2676, 2825, 2869
FokI GGATG 6 cut(s) 1128, 1205, 1514, 2166, 2185, 2771
FriOI GRGCYC 1 cut(s) 2841
Fsp4HI GCNGC 9 cut(s) 177, 1523, 1614, 2295, 2600, 2666, 2676, 2825, 2869
FspBI CTAG 6 cut(s) 275, 314, 429, 1133, 1151, 1625
FspI TGCGCA 1 cut(s) 956
GluI GCNGC 9 cut(s) 177, 1523, 1614, 2295, 2600, 2666, 2676, 2825, 2869
GsaI CCCAGC 2 cut(s) 2031, 2155
GsuI CTGGAG 2 cut(s) 1713, 1798
HaeII RGCGCY 4 cut(s) 182, 1821, 2671, 2874
HgaI GACGC 8 cut(s) 659, 1106, 1307, 1432, 1679, 2530, 2558, 2830
Hin1I GRCGYC 5 cut(s) 1443, 1575, 2008, 2522, 2822
HincII GTYRAC 2 cut(s) 226, 1579
HindII GTYRAC 2 cut(s) 226, 1579
HphI GGTGA 8 cut(s) 357, 695, 1352, 2407, 2704, 2804, 2870, 2909
Hpy166II GTNNAC 9 cut(s) 226, 619, 649, 1033, 1569, 1579, 1880, 1915, 1951
Hpy188I TCNGA 6 cut(s) 283, 445, 906, 1458, 1680, 2006
Hpy188III TCNNGA 8 cut(s) 547, 934, 970, 1290, 1496, 1730, 1922, 2233
Hpy8I GTNNAC 9 cut(s) 226, 619, 649, 1033, 1569, 1579, 1880, 1915, 1951
Hpy99I CGWCG 5 cut(s) 1580, 1695, 2010, 2524, 2897
HpyAV CCTTC 7 cut(s) 46, 214, 688, 1099, 1713, 2032, 2767
HpyCH4III ACNGT 7 cut(s) 672, 1919, 2075, 2087, 2454, 2562, 2897
HpyCH4IV ACGT 9 cut(s) 433, 818, 1370, 1452, 1575, 1597, 1882, 2008, 2503
HpySE526I ACGT 9 cut(s) 433, 818, 1370, 1452, 1575, 1597, 1882, 2008, 2503
Hsp92I GRCGYC 5 cut(s) 1443, 1575, 2008, 2522, 2822
Kpn2I TCCGGA 1 cut(s) 546
Kzo9I GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
LmnI GCTCC 5 cut(s) 424, 2004, 2320, 2644, 2863
Lsp1109I GCAGC 6 cut(s) 1509, 1600, 2306, 2611, 2662, 2677
LweI GCATC 7 cut(s) 504, 1094, 1147, 1227, 1929, 1980, 2611
MaeI CTAG 6 cut(s) 275, 314, 429, 1133, 1151, 1625
MaeII ACGT 9 cut(s) 433, 818, 1370, 1452, 1575, 1597, 1882, 2008, 2503
MaeIII GTNAC 4 cut(s) 363, 1604, 2504, 2800
MalI GATC 8 cut(s) 93, 842, 918, 930, 1344, 1725, 1781, 2177
MboI GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
MflI RGATCY 2 cut(s) 928, 1779
MhlI GDGCHC 3 cut(s) 236, 2649, 2841
MluCI AATT 8 cut(s) 386, 1044, 1109, 1179, 1849, 2345, 2568, 2686
MlyI GAGTC 7 cut(s) 287, 579, 960, 1192, 1413, 1805, 2598
MmeI TCCRAC 3 cut(s) 1155, 1305, 2029
Mph1103I ATGCAT 2 cut(s) 1087, 1220
MroI TCCGGA 1 cut(s) 546
MroXI GAANNNNTTC 2 cut(s) 1853, 2333
MseI TTAA 8 cut(s) 117, 204, 417, 1080, 2130, 2571, 2657, 2920
MslI CAYNNNNRTG 1 cut(s) 2114
MteI GCGCNGCGC 1 cut(s) 2869
Mva1269I GAATGC 2 cut(s) 1674, 2051
MvaI CCWGG 5 cut(s) 230, 756, 1267, 2137, 2288
NciI CCSGG 6 cut(s) 137, 357, 980, 1223, 1334, 1448
NcoI CCATGG 1 cut(s) 1740
NdeII GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
NheI GCTAGC 1 cut(s) 1624
NlaIV GGNNCC 9 cut(s) 426, 1053, 1758, 1781, 1969, 2000, 2311, 2838, 2839
NmuCI GTSAC 1 cut(s) 363
NruI TCGCGA 1 cut(s) 1923
NsbI TGCGCA 1 cut(s) 956
NsiI ATGCAT 2 cut(s) 1087, 1220
NspI RCATGY 4 cut(s) 355, 955, 1218, 2102
NspV TTCGAA 1 cut(s) 774
PaeI GCATGC 1 cut(s) 955
PasI CCCWGGG 1 cut(s) 1266
PauI GCGCGC 1 cut(s) 2529
PceI AGGCCT 1 cut(s) 2267
PcsI WCGNNNNNNNCGW 1 cut(s) 1703
PctI GAATGC 2 cut(s) 1674, 2051
PdmI GAANNNNTTC 2 cut(s) 1853, 2333
PfeI GAWTC 7 cut(s) 454, 709, 1459, 1469, 1540, 1831, 2443
PflFI GACNNNGTC 1 cut(s) 2552
PflMI CCANNNNNTGG 1 cut(s) 457
PfoI TCCNGGA 2 cut(s) 1221, 1446
PinAI ACCGGT 1 cut(s) 2804
PkrI GCNGC 9 cut(s) 178, 1524, 1615, 2296, 2601, 2667, 2677, 2826, 2870
Ple19I CGATCG 1 cut(s) 94
PleI GAGTC 7 cut(s) 286, 578, 960, 1192, 1413, 1805, 2597
PmaCI CACGTG 1 cut(s) 1598
PmlI CACGTG 1 cut(s) 1598
PpsI GAGTC 7 cut(s) 286, 578, 960, 1192, 1413, 1805, 2597
Ppu21I YACGTR 1 cut(s) 1598
PpuMI RGGWCCY 1 cut(s) 1051
PshBI ATTAAT 2 cut(s) 1080, 2571
Psp5II RGGWCCY 1 cut(s) 1051
Psp6I CCWGG 5 cut(s) 228, 754, 1265, 2135, 2286
PspCI CACGTG 1 cut(s) 1598
PspEI GGTNACC 1 cut(s) 2800
PspFI CCCAGC 2 cut(s) 2027, 2151
PspGI CCWGG 5 cut(s) 228, 754, 1265, 2135, 2286
PspN4I GGNNCC 9 cut(s) 426, 1053, 1758, 1781, 1969, 2000, 2311, 2838, 2839
PspOMI GGGCCC 1 cut(s) 2837
PspPI GGNCC 9 cut(s) 1051, 1561, 1982, 2025, 2087, 2309, 2484, 2837, 2838
PspPPI RGGWCCY 1 cut(s) 1051
PstI CTGCAG 1 cut(s) 697
PstNI CAGNNNCTG 1 cut(s) 457
PsuI RGATCY 2 cut(s) 928, 1779
PsyI GACNNNGTC 1 cut(s) 2552
PteI GCGCGC 1 cut(s) 2529
PvuI CGATCG 1 cut(s) 94
RruI TCGCGA 1 cut(s) 1923
RsaI GTAC 8 cut(s) 1164, 1513, 1752, 1950, 2359, 2366, 2471, 2494
RsaNI GTAC 8 cut(s) 1163, 1512, 1751, 1949, 2358, 2365, 2470, 2493
RseI CAYNNNNRTG 1 cut(s) 2114
SalI GTCGAC 1 cut(s) 1577
SaqAI TTAA 8 cut(s) 117, 204, 417, 1080, 2130, 2571, 2657, 2920
SatI GCNGC 9 cut(s) 177, 1523, 1614, 2295, 2600, 2666, 2676, 2825, 2869
Sau3AI GATC 8 cut(s) 91, 840, 916, 928, 1342, 1723, 1779, 2175
Sau96I GGNCC 9 cut(s) 1051, 1561, 1982, 2025, 2087, 2309, 2484, 2837, 2838
ScaI AGTACT 1 cut(s) 2359
SchI GAGTC 7 cut(s) 287, 579, 960, 1192, 1413, 1805, 2598
SduI GDGCHC 3 cut(s) 236, 2649, 2841
SfaNI GCATC 7 cut(s) 504, 1094, 1147, 1227, 1929, 1980, 2611
SfcI CTRYAG 4 cut(s) 693, 1638, 2195, 2220
SfuI TTCGAA 1 cut(s) 774
SgrAI CRCCGGYG 1 cut(s) 809
SinI GGWCC 4 cut(s) 1051, 1561, 1982, 2087
SmiMI CAYNNNNRTG 1 cut(s) 2114
SmlI CTYRAG 3 cut(s) 968, 1288, 2258
SmoI CTYRAG 3 cut(s) 968, 1288, 2258
SphI GCATGC 1 cut(s) 955
Sse9I AATT 8 cut(s) 386, 1044, 1109, 1179, 1849, 2345, 2568, 2686
SseBI AGGCCT 1 cut(s) 2267
SspMI CTAG 6 cut(s) 275, 314, 429, 1133, 1151, 1625
StuI AGGCCT 1 cut(s) 2267
StyI CCWWGG 2 cut(s) 198, 1740
TaaI ACNGT 7 cut(s) 672, 1919, 2075, 2087, 2454, 2562, 2897
TaiI ACGT 9 cut(s) 436, 821, 1373, 1455, 1578, 1600, 1885, 2011, 2506
TasI AATT 8 cut(s) 386, 1044, 1109, 1179, 1849, 2345, 2568, 2686
TatI WGTACW 1 cut(s) 2357
TauI GCSGC 3 cut(s) 179, 2827, 2871
TfiI GAWTC 7 cut(s) 454, 709, 1459, 1469, 1540, 1831, 2443
Tru1I TTAA 8 cut(s) 117, 204, 417, 1080, 2130, 2571, 2657, 2920
Tru9I TTAA 8 cut(s) 117, 204, 417, 1080, 2130, 2571, 2657, 2920
TscAI CASTG 8 cut(s) 22, 241, 271, 527, 697, 1198, 1791, 2459
TseFI GTSAC 1 cut(s) 363
TseI GCWGC 6 cut(s) 1522, 1613, 2294, 2599, 2665, 2675
Tsp45I GTSAC 1 cut(s) 363
TspGWI ACGGA 4 cut(s) 737, 1111, 1310, 2352
TspRI CASTG 8 cut(s) 22, 241, 271, 527, 697, 1198, 1791, 2459
Tth111I GACNNNGTC 1 cut(s) 2552
Van91I CCANNNNNTGG 1 cut(s) 457
VpaK11BI GGWCC 4 cut(s) 1051, 1561, 1982, 2087
VspI ATTAAT 2 cut(s) 1080, 2571
XagI CCTNNNNNAGG 2 cut(s) 2145, 2725
XceI RCATGY 4 cut(s) 355, 955, 1218, 2102
XcmI CCANNNNNNNNNTGG 1 cut(s) 1555
XmiI GTMKAC 3 cut(s) 1578, 1879, 1914
XmnI GAANNNNTTC 2 cut(s) 1853, 2333
XspI CTAG 6 cut(s) 275, 314, 429, 1133, 1151, 1625
ZraI GACGTC 2 cut(s) 1576, 2009
ZrmI AGTACT 1 cut(s) 2359
Zsp2I ATGCAT 2 cut(s) 1087, 1220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.