Rw5G012650

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000160
Physical Location & Seq
Forward (+)
14925717 .. 14932691
6975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G012650.1

Sequence Viewer

Length: 3432 bp
ATGGTTAAACGATTCAACGCCGATATCCCAGCAGATGAACCCAAACTCAATCTTGATGATGACATGGACGAAACAGAAATGGTGGATACCTCTTGCAATATGGTTTATGTGCTCCCTGCTAAGTATGCCTTACCAGTCGCTGCGCAGGAATGTGTAGAAACTGACGCGATTGGAGAACAGCAGTTGCAGATCACTTCAGCCGCAACAACGCAGGTGAAAGAAGCAGTGTTCCTTGAGACTAAAGATGCTAACAATAAGGGTTTCTTCATGAGCTTCACAAGACCCACACCCGCCATGGTGCAACACATGCGACCTTTGTATATCACAGTAGAAATGAATGGGACTAAGGTCAGCAAGATAATGGTAGAGGCCTTCCATTCTTTGTATGAGAGGCTATCCTCATACTTGGTGGAAACAGAGGCCTATGATCGAGTCGCGACCTTAGAAATTGTTAATGAAGAGTTCACGGATCAAGAAGAGGAGGAAGAAGAGATTCAGCTTGCTCCAGCGGCATTGGATGACACACCTCCGAAGGTTAGGGACCCTACTGAAAAGGTCAATCTGGGAACAACTGATGAGCCTATAGAAGTGGCTATCAGCACTTACTTAGAGCCTAGCGAGAAACAGAGGCTCGTTGAATTATTGCTGGAATTCAAGGACTGCTTCGCGGAAAAATACGAGGACATGCCAGGCTTATCACCAGACTTGGTATGCCACCAACTTCCAACGCTGCCTGATAAGAGGCCTGTGAAGCAAGAGCCGCGAAGAATGAACTCGGAGACCCAAGTTCTGGTCAAAGAAGAAGTCGAAAAGATGTACAAGTCAGGCATTATCAGGGTAGCCAAGTACAATAAGTGGTTGTCCAATATAGTGCCTGTTCGCAAGAAGAATGGCAAGATGAGGATCTGCGTAGATTACAGAGACCTTAATTTGGCTACACCTAAAGACGTCTACCCCATACCTGTAGCGGACATGTTGGTAGACGTAGTTGCAGGGCACGAACTGTTGTCTTTCATGGATGGTACAGCGGGATATCACCAGATTCCTGTCACAGAAGAAGATAGACACAAGACCGCATTCCGCTGCCCTGGGTTTGCAGGAGTTTTTGAATATGTGGTCATGCCTTTTGGACTGAAGAATGCTGGAGCAACATATCAGAGAGCCATGAACCTGATCTTCCACAACATACTTGGGAAGATCTTAGAGGTTTACATTGACGACGTCGCAGGAGATTTCCTGGGATTCATTGTCCATCAAAGAGGCATTGAGGTCCCTGAGGATAAAGCAAACGCGGTCATCAATGCATCTCCCCCGCGAACGAAGAAAGAGTTACAGCGACTACTGGGTAGGATCAACTTTTTGCGACGATTCATTTCTAACTCTTCAGGTAAAATCCATCCGTTTTCCCCACTGTTGAAGTTGCAAGGACAGAATGAGTTTGTGTGGGAACCTAAACATCAAGAGGCTTTCGACAAAATCAAGGCCTATCTGGCGAGCCCGCCAGTGCTTGTTCCTCCTAGAGCGGGATTTCCATTAAAGTTGTATGTTTCAGCAGCTGAGGCTTCCATTGGCAGCCTCCTCGCTCAGGATGATGCAAATGGTGTTGAACATGCCATCTTTTACCTCAGTAGGACACTCACAGATTGCGAAACAAGGTACACTCCAATGGAAAAGCTGTGTCTTACATTATACTTCTCAGCATGCAAGTTGCGGCATTACATGTTGTCCTTTACTACTTGCATCATTGCTCAAACCGATCTAGTCAAATATATGCTGTCGCGACCTATCTTGAGAGGCCGTATTGGCAAGTGGGTGCTCGCATTATCAGAATTCTCGCTACAGTATGTGCCACAGAAAGCAGTAAAGGGGCAAGCCATAGCAGACTTTCTGGCACATCACCCTATGCTGGATGTCCCCGCGGTTAGAGATTTAGAGAGTACGCCGCTTTATCAAGCCACAGTCTCACTCCAGCCTTGGGTGTTATATTTTGACGGGTCAAGAACAGATACGCTAGCAGGAGCAGGGGTTGTCTTGGAAAACCCGGCCGGCGATCGATTTTCCTACTCTTTCCAGTTGGAGTTCCAGTGTACCAATAACCAAGCAGAGTATGAGGCCCTCATTATAGGCCTAGAAGTACTACTGGAAATGGGAATCGGAGATGTACAAATACTTGGCGACTCTCTCTTGGTTATCAATCAGTTGTGCAACGAGTTCAGATGCAATAGCTTCACGTTGGTTCCTTATTGGAACAGGGCATTAGACCTGTTGGACCAGTTTGATAACATACATCTAGAGTACATTCCTCGCGAGCGAAATTTTGCAGCGAACGAGTTGGCCCAACTGGCAACAGGGGTAACATTGAGATATGGCGTGCGGGAGAGAATCCTTAAAGTCGAGCGTCGCACGCTTCCTTCATGGATGGCGAGAAGAGATCCTCCAGATGATACCTTAGTCGCGACCCTGGAACCCATTGACGTGGATTGGCGCATCCCTTTGATCGCTTATCTCAAGCAGCCAGATCCCACTGCCGACAGGAAGATTCGTTTTCTTGCACTAAATTACTTTCTTAGAGGTGACGAGCTGCGACGACGAGGCGAAGATGGCATAGACTTCAGATGTGTTTATGGTCGCGAAGCGAAACAACTCATGCGCGAAGTTCATTCCGGCATTTGCGGTGCTCACCAAGCAGGTCCAAAGATGCGATGGTTGCTCAGACGACATGGATATTTTTGGCCCAAAGTTTTTGTTTCGGACCGGGGGGCAGCTTTCATGGGTGGTGAAGTTGATAAACTGGCAAAGGAATGGGGAATACAGTTCGTCCATTCCAGTCCTTATTATGCTCAGTCTAACGGTCAAGCGGAGGCCAGCAATAAAATCATCATCACTTTGCTGAAGAAAATGTTGGAAGCTAATCCGCGACAATGGCATGAGACCCTTTATGAGACTCTTTGGGCCTACCGCACATCGAAGCGAAATCCCACCGCGACTACACCTTATGCTTTGATGTTTGGCCATGACGCAGTCCTGCCTTTGGAAGTCAACGTCCAATCATTACGAGTCCAAGAGCAACATCATCTCATTGGAGAAGACTACGTTCAGGCTATGTGGCAGGAACATGAAGACCTTAGCGAAAAGCGCTTTGAGGCTTTAGATAGTTTGGTCATGGAAAAGCAACGAGTCGCTCGAGCCTATGACAAGCGAACAAGGGGAAGGAATTACAGCGAAGGAGAGTTGGTTTGGAAAGCAGTTCTCCCGCTTGGCGAAAAATTAGATGGTCGCGGCAAATGGACTCCAAGATGGGAAGGCCCGTACATCATTCATAAAATCTTAGGGAAAGGAGCTTTTCATCTCAAGGACCTGGATGGAGATGTCCATCATAACCCTATCAATGGGAAATACTTTCTCAATGTCTGGGATTCAGAGGAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

1143

Amino Acids

130.41

Weight (kDa)

5.72

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 293 - 408 2e-18 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 482 - 576 7.7e-21 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 511 - 611 4.8e-16 RNase H-like domain found in reverse transcriptase
RVT_3 PF13456 663 - 781 5.9e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 202
AatII GACGTC 2 cut(s) 951, 1224
Acc16I TGCGCA 1 cut(s) 144
Acc36I ACCTGC 2 cut(s) 202, 2678
AccB7I CCANNNNNTGG 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 1523
AccI GTMKAC 2 cut(s) 951, 981
AclWI GGATC 5 cut(s) 477, 911, 1358, 2426, 2513
AcoI YGGCCR 2 cut(s) 2043, 3010
AcsI RAATTY 3 cut(s) 650, 1829, 2314
AcuI CTGAAG 5 cut(s) 180, 1154, 1368, 2596, 2912
AcyI GRCGYC 2 cut(s) 948, 1221
AfeI AGCGCT 1 cut(s) 3137
AfiI CCNNNNNNNGG 8 cut(s) 790, 931, 1523, 1585, 1906, 1975, 3031, 3389
AflIII ACRYGT 2 cut(s) 972, 1719
AgsI TTSAA 6 cut(s) 16, 638, 655, 1109, 1417, 1607
AjiI CACGTC 1 cut(s) 2476
AjnI CCWGG 5 cut(s) 688, 1087, 1236, 2460, 3357
AjuI GAANNNNNNNTTGG 2 cut(s) 2885, 2917
AluBI AGCT 9 cut(s) 273, 499, 1556, 1675, 2226, 2581, 2765, 2909, 3341
AluI AGCT 9 cut(s) 273, 499, 1556, 1675, 2226, 2581, 2765, 2909, 3341
Alw21I GWGCWC 3 cut(s) 114, 1818, 2680
Alw26I GTCTC 6 cut(s) 230, 773, 915, 1966, 2924, 2936
AlwI GGATC 5 cut(s) 477, 911, 1358, 2426, 2513
AlwNI CAGNNNCTG 2 cut(s) 140, 1556
Ama87I CYCGRG 1 cut(s) 3183
Aor51HI AGCGCT 1 cut(s) 3137
ApeKI GCWGC 9 cut(s) 140, 730, 1083, 1553, 1572, 2321, 2512, 2581, 2762
ApoI RAATTY 3 cut(s) 650, 1829, 2314
ArsI GACNNNNNNTTYG 2 cut(s) 789, 821
Asp700I GAANNNNTTC 1 cut(s) 492
AspLEI GCGC 4 cut(s) 145, 2487, 2652, 3138
AsuC2I CCSGG 2 cut(s) 2042, 2756
AsuHPI GGTGA 7 cut(s) 226, 690, 1028, 1889, 2585, 2672, 2789
AsuNHI GCTAGC 1 cut(s) 2011
AvaI CYCGRG 1 cut(s) 3183
AvaII GGWCC 6 cut(s) 541, 1270, 2269, 2690, 2752, 3355
AxyI CCTNAGG 1 cut(s) 1275
BaeGI GKGCMC 1 cut(s) 999
BaeI ACNNNNGTAYC 2 cut(s) 2436, 2469
BalI TGGCCA 1 cut(s) 3012
BanII GRGCYC 1 cut(s) 1499
BbsI GAAGAC 2 cut(s) 3093, 3126
Bbv12I GWGCWC 3 cut(s) 114, 1818, 2680
BbvCI CCTCAGC 1 cut(s) 1557
BbvI GCAGC 9 cut(s) 127, 717, 1070, 1565, 1584, 2333, 2524, 2568, 2774
BceAI ACGGC 1 cut(s) 1782
BcgI CGANNNNNNTGC 2 cut(s) 1561, 1595
BciT130I CCWGG 5 cut(s) 690, 1089, 1238, 2462, 3359
BciVI GTATCC 1 cut(s) 79
BcnI CCSGG 2 cut(s) 2042, 2756
BcoDI GTCTC 6 cut(s) 230, 773, 915, 1966, 2924, 2936
BfaI CTAG 6 cut(s) 615, 1518, 1760, 2012, 2129, 2291
BfmI CTRYAG 3 cut(s) 582, 963, 1838
BfoI RGCGCY 1 cut(s) 3139
BfuAI ACCTGC 2 cut(s) 202, 2678
BfuI GTATCC 1 cut(s) 79
BglI GCCNNNNNGGC 3 cut(s) 1490, 1803, 2342
BglII AGATCT 1 cut(s) 1197
BmcAI AGTACT 1 cut(s) 2136
Bme1390I CCNGG 7 cut(s) 690, 1089, 1238, 2042, 2462, 2756, 3359
Bme18I GGWCC 6 cut(s) 541, 1270, 2269, 2690, 2752, 3355
BmeT110I CYCGRG 1 cut(s) 3183
BmgBI CACGTC 1 cut(s) 2476
BmiI GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2238, 2466
BmrFI CCNGG 7 cut(s) 690, 1089, 1238, 2042, 2462, 2756, 3359
BmrI ACTGGG 1 cut(s) 1352
BmsI GCATC 7 cut(s) 235, 1313, 1582, 1749, 2207, 2496, 2688
BmtI GCTAGC 1 cut(s) 2015
BmuI ACTGGG 1 cut(s) 1352
BoxI GACNNNNGTC 1 cut(s) 347
BpiI GAAGAC 2 cut(s) 3093, 3126
BpmI CTGGAG 4 cut(s) 489, 1164, 1952, 2421
Bpu10I CCTNAGC 3 cut(s) 1557, 1584, 3125
BpuEI CTTGAG 4 cut(s) 254, 1810, 2492, 3335
BpuMI CCSGG 2 cut(s) 2042, 2756
Bsa29I ATCGAT 1 cut(s) 2053
BsaBI GATNNNNATC 2 cut(s) 902, 3372
BsaHI GRCGYC 2 cut(s) 948, 1221
BsaI GGTCTC 3 cut(s) 773, 915, 2924
BsaJI CCNNGG 8 cut(s) 294, 1087, 1088, 1237, 1917, 1973, 2460, 2755
BsaXI ACNNNNNCTCC 6 cut(s) 2010, 2040, 2069, 2099, 3219, 3249
Bsc4I CCNNNNNNNGG 8 cut(s) 790, 931, 1523, 1585, 1906, 1975, 3031, 3389
Bse118I RCCGGY 1 cut(s) 2045
Bse21I CCTNAGG 1 cut(s) 1275
Bse3DI GCAATG 1 cut(s) 1743
Bse8I GATNNNNATC 2 cut(s) 902, 3372
BseBI CCWGG 5 cut(s) 690, 1089, 1238, 2462, 3359
BseCI ATCGAT 1 cut(s) 2053
BseDI CCNNGG 8 cut(s) 294, 1087, 1088, 1237, 1917, 1973, 2460, 2755
BseGI GGATG 8 cut(s) 523, 1024, 1396, 1594, 1915, 2424, 2487, 3367
BseJI GATNNNNATC 2 cut(s) 902, 3372
BseLI CCNNNNNNNGG 8 cut(s) 790, 931, 1523, 1585, 1906, 1975, 3031, 3389
BseMI GCAATG 1 cut(s) 1743
BseMII CTCAG 7 cut(s) 1266, 1548, 1598, 1639, 1710, 2725, 2855
BseRI GAGGAG 2 cut(s) 494, 1568
BseSI GKGCMC 1 cut(s) 999
BseX3I CGGCCG 1 cut(s) 2043
BseXI GCAGC 9 cut(s) 127, 717, 1070, 1565, 1584, 2333, 2524, 2568, 2774
BseYI CCCAGC 1 cut(s) 28
Bsh1285I CGRYCG 2 cut(s) 2046, 2053
BshVI ATCGAT 1 cut(s) 2053
BsiEI CGRYCG 2 cut(s) 2046, 2053
BsiHKAI GWGCWC 3 cut(s) 114, 1818, 2680
BsiHKCI CYCGRG 1 cut(s) 3183
BsiSI CCGG 4 cut(s) 2042, 2046, 2664, 2755
BslFI GGGAC 4 cut(s) 355, 554, 1256, 1898
BslI CCNNNNNNNGG 8 cut(s) 790, 931, 1523, 1585, 1906, 1975, 3031, 3389
BsmAI GTCTC 6 cut(s) 230, 773, 915, 1966, 2924, 2936
BsmFI GGGAC 4 cut(s) 355, 554, 1256, 1898
BsmI GAATGC 2 cut(s) 1076, 1144
Bso31I GGTCTC 3 cut(s) 773, 915, 2924
BsoBI CYCGRG 1 cut(s) 3183
Bsp1286I GDGCHC 5 cut(s) 114, 999, 1499, 1818, 2680
Bsp1407I TGTACA 2 cut(s) 816, 2161
Bsp19I CCATGG 1 cut(s) 294
Bsp68I TCGCGA 5 cut(s) 437, 1780, 2307, 2456, 2631
BspCNI CTCAG 7 cut(s) 1267, 1549, 1597, 1638, 1709, 2724, 2854
BspDI ATCGAT 1 cut(s) 2053
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2238, 2466
BspMI ACCTGC 2 cut(s) 202, 2678
BspOI GCTAGC 1 cut(s) 2015
BspPI GGATC 5 cut(s) 477, 911, 1358, 2426, 2513
BspTNI GGTCTC 3 cut(s) 773, 915, 2924
BsrBI CCGCTC 1 cut(s) 1523
BsrDI GCAATG 1 cut(s) 1743
BsrFI RCCGGY 1 cut(s) 2045
BsrGI TGTACA 2 cut(s) 816, 2161
BssAI RCCGGY 1 cut(s) 2045
BssECI CCNNGG 8 cut(s) 294, 1087, 1088, 1237, 1917, 1973, 2460, 2755
BssNI GRCGYC 2 cut(s) 948, 1221
BssT1I CCWWGG 2 cut(s) 294, 1973
Bst2UI CCWGG 5 cut(s) 690, 1089, 1238, 2462, 3359
Bst4CI ACNGT 7 cut(s) 328, 1005, 1413, 1842, 1960, 2814, 2852
Bst6I CTCTTC 5 cut(s) 453, 471, 483, 1387, 2422
BstACI GRCGYC 2 cut(s) 948, 1221
BstAPI GCANNNNNTGC 1 cut(s) 307
BstAUI TGTACA 2 cut(s) 816, 2161
BstDSI CCRYGG 2 cut(s) 294, 1917
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 8 cut(s) 523, 1024, 1396, 1594, 1915, 2424, 2487, 3367
BstH2I RGCGCY 1 cut(s) 3139
BstHHI GCGC 4 cut(s) 145, 2487, 2652, 3138
BstMAI GTCTC 6 cut(s) 230, 773, 915, 1966, 2924, 2936
BstMCI CGRYCG 2 cut(s) 2046, 2053
BstNI CCWGG 5 cut(s) 690, 1089, 1238, 2462, 3359
BstNSI RCATGY 6 cut(s) 310, 688, 976, 1613, 1704, 1723
BstPAI GACNNNNGTC 1 cut(s) 347
BstSCI CCNGG 7 cut(s) 688, 1087, 1236, 2040, 2460, 2754, 3357
BstSFI CTRYAG 3 cut(s) 582, 963, 1838
BstSLI GKGCMC 1 cut(s) 999
BstV1I GCAGC 9 cut(s) 127, 717, 1070, 1565, 1584, 2333, 2524, 2568, 2774
BstV2I GAAGAC 2 cut(s) 3093, 3126
BstX2I RGATCY 4 cut(s) 903, 1197, 2431, 2518
BstXI CCANNNNNNTGG 1 cut(s) 2476
BstYI RGATCY 4 cut(s) 903, 1197, 2431, 2518
BstZI CGGCCG 1 cut(s) 2043
Bsu15I ATCGAT 1 cut(s) 2053
Bsu36I CCTNAGG 1 cut(s) 1275
BsuI GTATCC 1 cut(s) 79
BsuTUI ATCGAT 1 cut(s) 2053
BtgI CCRYGG 2 cut(s) 294, 1917
BtgZI GCGATG 1 cut(s) 2716
BtrI CACGTC 1 cut(s) 2476
BtsCI GGATG 8 cut(s) 523, 1024, 1396, 1594, 1915, 2424, 2487, 3367
BtsI GCAGTG 2 cut(s) 231, 2523
BtsIMutI CAGTG 5 cut(s) 231, 1409, 1509, 2090, 2523
BtuMI TCGCGA 5 cut(s) 437, 1780, 2307, 2456, 2631
BveI ACCTGC 2 cut(s) 202, 2678
CaiI CAGNNNCTG 2 cut(s) 140, 1556
CciI TCATGA 1 cut(s) 267
CfoI GCGC 4 cut(s) 145, 2487, 2652, 3138
Cfr10I RCCGGY 1 cut(s) 2045
Cfr42I CCGCGG 1 cut(s) 1920
ClaI ATCGAT 1 cut(s) 2053
CpoI CGGWCCG 1 cut(s) 2752
CseI GACGC 3 cut(s) 173, 2387, 3026
CspI CGGWCCG 1 cut(s) 2752
EaeI YGGCCR 2 cut(s) 2043, 3010
EagI CGGCCG 1 cut(s) 2043
Eam1104I CTCTTC 5 cut(s) 453, 471, 483, 1387, 2422
EarI CTCTTC 5 cut(s) 453, 471, 483, 1387, 2422
EclXI CGGCCG 1 cut(s) 2043
Eco130I CCWWGG 2 cut(s) 294, 1973
Eco147I AGGCCT 5 cut(s) 371, 422, 745, 1484, 2127
Eco24I GRGCYC 1 cut(s) 1499
Eco31I GGTCTC 3 cut(s) 773, 915, 2924
Eco32I GATATC 2 cut(s) 25, 1034
Eco47I GGWCC 6 cut(s) 541, 1270, 2269, 2690, 2752, 3355
Eco47III AGCGCT 1 cut(s) 3137
Eco52I CGGCCG 1 cut(s) 2043
Eco57I CTGAAG 5 cut(s) 180, 1154, 1368, 2596, 2912
Eco81I CCTNAGG 1 cut(s) 1275
Eco88I CYCGRG 1 cut(s) 3183
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoO109I RGGNCCY 4 cut(s) 541, 1270, 2113, 3355
EcoRI GAATTC 2 cut(s) 650, 1829
EcoRII CCWGG 5 cut(s) 688, 1087, 1236, 2460, 3357
EcoRV GATATC 2 cut(s) 25, 1034
EcoT14I CCWWGG 2 cut(s) 294, 1973
EcoT22I ATGCAT 1 cut(s) 1306
EcoT38I GRGCYC 1 cut(s) 1499
ErhI CCWWGG 2 cut(s) 294, 1973
FalI AAGNNNNNCTT 6 cut(s) 113, 145, 248, 280, 647, 679
FaqI GGGAC 4 cut(s) 355, 554, 1256, 1898
FauI CCCGC 8 cut(s) 298, 1021, 1320, 1506, 1516, 1924, 2367, 3261
FblI GTMKAC 2 cut(s) 951, 981
FokI GGATG 8 cut(s) 530, 1031, 1383, 1601, 1922, 2431, 2474, 3374
FriOI GRGCYC 1 cut(s) 1499
FspBI CTAG 6 cut(s) 615, 1518, 1760, 2012, 2129, 2291
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 4 cut(s) 144, 2486, 2651, 3137
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 4 cut(s) 489, 1164, 1952, 2421
HaeII RGCGCY 1 cut(s) 3139
HapII CCGG 4 cut(s) 2042, 2046, 2664, 2755
HgaI GACGC 3 cut(s) 173, 2387, 3026
HhaI GCGC 4 cut(s) 145, 2487, 2652, 3138
Hin1I GRCGYC 2 cut(s) 948, 1221
Hin6I GCGC 4 cut(s) 143, 2485, 2650, 3136
HinP1I GCGC 4 cut(s) 143, 2485, 2650, 3136
HincII GTYRAC 1 cut(s) 3040
HindII GTYRAC 1 cut(s) 3040
HpaII CCGG 4 cut(s) 2042, 2046, 2664, 2755
HphI GGTGA 7 cut(s) 226, 690, 1028, 1889, 2585, 2672, 2789
Hpy166II GTNNAC 7 cut(s) 465, 952, 982, 1210, 1659, 2088, 3040
Hpy8I GTNNAC 7 cut(s) 465, 952, 982, 1210, 1659, 2088, 3040
Hpy99I CGWCG 6 cut(s) 1223, 1226, 1368, 2403, 2589, 2592
HpyAV CCTTC 6 cut(s) 382, 526, 2421, 3204, 3218, 3296
HpyCH4III ACNGT 7 cut(s) 328, 1005, 1413, 1842, 1960, 2814, 2852
HpyCH4IV ACGT 7 cut(s) 948, 984, 1221, 2231, 2475, 3042, 3093
HpySE526I ACGT 7 cut(s) 948, 984, 1221, 2231, 2475, 3042, 3093
Hsp92I GRCGYC 2 cut(s) 948, 1221
HspAI GCGC 4 cut(s) 143, 2485, 2650, 3136
KflI GGGWCCC 1 cut(s) 541
KroI GCCGGC 1 cut(s) 2045
KroNI GCCGGC 1 cut(s) 2047
KspI CCGCGG 1 cut(s) 1920
LmnI GCTCC 5 cut(s) 117, 508, 1145, 2018, 3338
Lsp1109I GCAGC 9 cut(s) 127, 717, 1070, 1565, 1584, 2333, 2524, 2568, 2774
LweI GCATC 7 cut(s) 235, 1313, 1582, 1749, 2207, 2496, 2688
MaeI CTAG 6 cut(s) 615, 1518, 1760, 2012, 2129, 2291
MaeII ACGT 7 cut(s) 948, 984, 1221, 2231, 2475, 3042, 3093
MaeIII GTNAC 4 cut(s) 1048, 1329, 2353, 2573
MbiI CCGCTC 1 cut(s) 1523
MflI RGATCY 4 cut(s) 903, 1197, 2431, 2518
MhlI GDGCHC 5 cut(s) 114, 999, 1499, 1818, 2680
MlsI TGGCCA 1 cut(s) 3012
MluCI AATT 9 cut(s) 447, 638, 650, 928, 1829, 2314, 2557, 3214, 3266
MluNI TGGCCA 1 cut(s) 3012
MlyI GAGTC 6 cut(s) 441, 2171, 2938, 3066, 3186, 3283
MmeI TCCRAC 4 cut(s) 749, 2055, 2247, 2883
Mox20I TGGCCA 1 cut(s) 3012
Mph1103I ATGCAT 1 cut(s) 1306
MroNI GCCGGC 1 cut(s) 2045
MroXI GAANNNNTTC 1 cut(s) 492
MscI TGGCCA 1 cut(s) 3012
MseI TTAA 5 cut(s) 6, 453, 927, 1535, 2388
MslI CAYNNNNRTG 2 cut(s) 1664, 2474
Msp20I TGGCCA 1 cut(s) 3012
MspA1I CMGCKG 5 cut(s) 509, 1028, 1083, 1556, 1919
MspI CCGG 4 cut(s) 2042, 2046, 2664, 2755
MspR9I CCNGG 7 cut(s) 690, 1089, 1238, 2042, 2462, 2756, 3359
Mva1269I GAATGC 2 cut(s) 1076, 1144
MvaI CCWGG 5 cut(s) 690, 1089, 1238, 2462, 3359
NaeI GCCGGC 1 cut(s) 2047
NciI CCSGG 2 cut(s) 2042, 2756
NcoI CCATGG 1 cut(s) 294
NgoMIV GCCGGC 1 cut(s) 2045
NheI GCTAGC 1 cut(s) 2011
NlaIV GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2238, 2466
NmuCI GTSAC 2 cut(s) 1048, 2573
NruI TCGCGA 5 cut(s) 437, 1780, 2307, 2456, 2631
NsbI TGCGCA 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 1306
NspI RCATGY 6 cut(s) 310, 688, 976, 1613, 1704, 1723
PaeI GCATGC 1 cut(s) 1704
PaeR7I CTCGAG 1 cut(s) 3183
PagI TCATGA 1 cut(s) 267
PaqCI CACCTGC 1 cut(s) 202
PasI CCCWGGG 1 cut(s) 1088
PceI AGGCCT 5 cut(s) 371, 422, 745, 1484, 2127
PciI ACATGT 2 cut(s) 972, 1719
PcsI WCGNNNNNNNCGW 1 cut(s) 3181
PctI GAATGC 2 cut(s) 1076, 1144
PdiI GCCGGC 1 cut(s) 2047
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 9 cut(s) 12, 493, 1042, 1242, 1368, 2151, 2382, 2539, 3416
PflFI GACNNNGTC 2 cut(s) 1220, 3020
PflMI CCANNNNNTGG 1 cut(s) 790
Ple19I CGATCG 1 cut(s) 2053
PleI GAGTC 6 cut(s) 440, 2171, 2938, 3065, 3185, 3283
PpsI GAGTC 6 cut(s) 440, 2171, 2938, 3065, 3185, 3283
PpuMI RGGWCCY 3 cut(s) 541, 1270, 3355
PscI ACATGT 2 cut(s) 972, 1719
PshAI GACNNNNGTC 1 cut(s) 347
Psp5II RGGWCCY 3 cut(s) 541, 1270, 3355
Psp6I CCWGG 5 cut(s) 688, 1087, 1236, 2460, 3357
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 5 cut(s) 688, 1087, 1236, 2460, 3357
PspN4I GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2238, 2466
PspPPI RGGWCCY 3 cut(s) 541, 1270, 3355
PspXI VCTCGAGB 1 cut(s) 3183
PstNI CAGNNNCTG 2 cut(s) 140, 1556
PsuI RGATCY 4 cut(s) 903, 1197, 2431, 2518
PsyI GACNNNGTC 2 cut(s) 1220, 3020
PvuI CGATCG 1 cut(s) 2053
PvuII CAGCTG 1 cut(s) 1556
RruI TCGCGA 5 cut(s) 437, 1780, 2307, 2456, 2631
RseI CAYNNNNRTG 2 cut(s) 1664, 2474
Rsr2I CGGWCCG 1 cut(s) 2752
RsrII CGGWCCG 1 cut(s) 2752
SacII CCGCGG 1 cut(s) 1920
SaqAI TTAA 5 cut(s) 6, 453, 927, 1535, 2388
ScaI AGTACT 1 cut(s) 2136
SchI GAGTC 6 cut(s) 441, 2171, 2938, 3066, 3186, 3283
ScrFI CCNGG 7 cut(s) 690, 1089, 1238, 2042, 2462, 2756, 3359
SduI GDGCHC 5 cut(s) 114, 999, 1499, 1818, 2680
SfaNI GCATC 7 cut(s) 235, 1313, 1582, 1749, 2207, 2496, 2688
SfcI CTRYAG 3 cut(s) 582, 963, 1838
Sfr274I CTCGAG 1 cut(s) 3183
Sfr303I CCGCGG 1 cut(s) 1920
SgrBI CCGCGG 1 cut(s) 1920
SinI GGWCC 6 cut(s) 541, 1270, 2269, 2690, 2752, 3355
SlaI CTCGAG 1 cut(s) 3183
SmiMI CAYNNNNRTG 2 cut(s) 1664, 2474
SmlI CTYRAG 5 cut(s) 233, 1789, 2507, 3183, 3350
SmoI CTYRAG 5 cut(s) 233, 1789, 2507, 3183, 3350
SphI GCATGC 1 cut(s) 1704
Sse9I AATT 9 cut(s) 447, 638, 650, 928, 1829, 2314, 2557, 3214, 3266
SseBI AGGCCT 5 cut(s) 371, 422, 745, 1484, 2127
SspMI CTAG 6 cut(s) 615, 1518, 1760, 2012, 2129, 2291
StuI AGGCCT 5 cut(s) 371, 422, 745, 1484, 2127
StyD4I CCNGG 7 cut(s) 688, 1087, 1236, 2040, 2460, 2754, 3357
StyI CCWWGG 2 cut(s) 294, 1973
TaaI ACNGT 7 cut(s) 328, 1005, 1413, 1842, 1960, 2814, 2852
TaiI ACGT 7 cut(s) 951, 987, 1224, 2234, 2478, 3045, 3096
TaqI TCGA 7 cut(s) 430, 807, 1470, 2053, 2394, 2966, 3184
TasI AATT 9 cut(s) 447, 638, 650, 928, 1829, 2314, 2557, 3214, 3266
TatI WGTACW 5 cut(s) 816, 846, 2134, 2161, 2295
TauI GCSGC 6 cut(s) 203, 512, 763, 1714, 1945, 3282
TfiI GAWTC 9 cut(s) 12, 493, 1042, 1242, 1368, 2151, 2382, 2539, 3416
Tru1I TTAA 5 cut(s) 6, 453, 927, 1535, 2388
Tru9I TTAA 5 cut(s) 6, 453, 927, 1535, 2388
TscAI CASTG 5 cut(s) 231, 1416, 1509, 2090, 2530
TseFI GTSAC 2 cut(s) 1048, 2573
TseI GCWGC 9 cut(s) 140, 730, 1083, 1553, 1572, 2321, 2512, 2581, 2762
Tsp45I GTSAC 2 cut(s) 1048, 2573
TspGWI ACGGA 2 cut(s) 482, 1389
TspRI CASTG 5 cut(s) 231, 1416, 1509, 2090, 2530
Tth111I GACNNNGTC 2 cut(s) 1220, 3020
Van91I CCANNNNNTGG 1 cut(s) 790
VpaK11BI GGWCC 6 cut(s) 541, 1270, 2269, 2690, 2752, 3355
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 3 cut(s) 650, 1829, 2314
XbaI TCTAGA 1 cut(s) 2290
XceI RCATGY 6 cut(s) 310, 688, 976, 1613, 1704, 1723
XcmI CCANNNNNNNNNTGG 3 cut(s) 292, 1187, 2700
XhoI CTCGAG 1 cut(s) 3183
XmiI GTMKAC 2 cut(s) 951, 981
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 6 cut(s) 615, 1518, 1760, 2012, 2129, 2291
ZraI GACGTC 2 cut(s) 949, 1222
ZrmI AGTACT 1 cut(s) 2136
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.