Rw2G002740

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000160
Physical Location & Seq
Forward (+)
2427834 .. 2432296
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G002740.1

Sequence Viewer

Length: 3456 bp
ATGGCTAAACAGTTCAACGCCGAAATCCCAACAGATGAACCCAAGCTCAATCTTGATGATGACATGGACGAAACAGAAATGGTGGATACCTCTTGCAATATGGTTTATGTGCTTCCTGCTAAGTATGCCTTACCAGTCGCTGCGCAGGAATGCGTAGAGGCTGACACGATTGGAGAACAGCAGTTGCAGATCACTTCAGCCGCAACAACGCCGATAAAAGAAGCAGTGTTCCTTGAGACTGAAGATGCTAACAGCAAGGGTTTCTTTATGAGCTTCACAAGACCCACTCCTGCCATGGTGCAACACATGCGGCCTTTGTATATCACAGCGGAAATTAATGGGACTAAGGTCAGCAAGATAATGGCCTTCCATTCCTTGTACGAGAGGCTATCATCATACTTGGTGGAAAAAGAGGCCTATGATCGAGTCGCGACCTTAGAAATTGTTAATGAAGAGTTCTCTGACCAAGAAGAGGAGGAAGAAGAGATTCAGATTGCTCCAGCGGCATTGGATGACACACCTCCGAAGGTTAGGGACCCTACTGAGAAAGTCAATCTGGGAACAACTGATGAGCCTATAGAAGTGGCTATCAGCGCTTACTTAGAGCCTAGCGAGAAACAGAGGCTCGTCGACTTATTGTTGGAATTCAAGGACTGCTTCGCGGAAAAATACGAAGACATGCCAGGCCTATCACCAGACTTAGTATGCCACCAACTACCAACGTTGCCTGATAAGAGGCCTGTGAAGCAAGAGCCGCGAAGAATGAACTCGGAAACCCAAGTTCTGGTCAAAGAGGAAGTCGAAAAGATGCACAAGTCGGGCATTATCAGGGTAGCCAAGTACAATCAGTGGCTGTCCAATATAGTGCCTGTTCGCAAGAAGAATGGCAAGATAAGGGTCTGCGTGGATTACAGAGACCTTAATTTGGCCACACCTAAAGACGTCTACCCCATGCCGGTTGCGGACATGTTGGTAGACGCAGTTGCAGGGCACGAACTGTTGTCTTTCATGGACGGCACAGCGGGATATCACCAAATTCCGGTCGCGGAGGAAGATAGACACAAGACTGCATTCCGCTGCCCTGGGTTCGCAGGAGTTTTTGAGTATGTGGTCATGCCTTTTGGACTAAAGAATGCCGGAGCAACATATCAGAGAGCCATGAACCTGATCTTCCACGACATACTTGGGAAGATCTTAGAGGTTTACATTGATGACGTCGCAGGAGATTTCCTGGGATTCATTGTCCATCAAAGAGGCATTGAGGTCCCTGAGGATAAAGCAAACGCGAAAGAGTTACAGCGACTACTGGGTAGGATCAACTTTTTGCGACGATTCATTTCTAACTCTTCAGGCAAAATCCATCCGTTTTCCCCACTGTTGAAGTTGCAAGGACAGAATGAGTTTGTGTGGGAACCTAAACATCAAGAGGCTTTCGACAAAATCAAGGCCTATCTGGCGAGCCCGCCAGTGCTTGTTCCTCCTAGAGCGGGATTTCCATTAAAGTTGTATGTTTCAGCAGCTGAGGCTTCCATTGGCAGCCTCCTCGCTCAGGATGATGCAAATGGTGTTGAACATGCCATCTTTTACCTCAGTAGGACACTCACAGATTGCGAAACAAGGTACACTCCAATGGAAAAGCTGTGTCTTACATTATACTTCTCAGCATGCAAGTTGCGGCATTACATGTTGTCCTTTACTACTTGCATCATTGCTCAAACCGATCTAGTCAAATATATGCTGTCGCGACCTATCTTGAGAGGCCGTATTGGCAAGTGGGTGCTCGCATTATCAGAATTCTCGCTACAGTATGTGCCACAGAAAGCAGTAAAGGGGCAAGCCATAGCAGACTTTCTGGCACATCACCCTATGCTGGATGTCCCCGCGGTTAGAGATTTAGAGGTCGCTGCCACAACTTTAGATCGCCCGGATTTAGCGTGCTTGCCAGAGTACGCCGCGCTTTATCAAGCCACAGTCTCACTCCAGCCTTGGGTGTTATATTTTGACGGGTCAAGAACAGATACGCTAGCAGGAGCAGGGGTTGTCTTGGAAAACCCGGCCGGCGATCGATTTTCCTACTCTTTCCAGTTGGAGTTCCAGTGTACCAATAACCAAGCAGAGTATGAGGCCCTCATTATAGGCCTAGAAGTACTACTGGAAATGGGAATCAGGGATGTACAAATACTTGGCGACTCTCTCTTGGTTATCAATCAGTTGTGCAACGAGTTCAGATGCAATAGCTTCACGTTGGTTCCTTATTGGAACAGGGCATTGTACCTGTTGGACCAGTTTGATAACATACATCTAGAGTACATTCCTCGCGAGCGAAATTTTGCAGCGAACGAGTTGGCCCAACTGGCAACAGGGGTAACATTGAGATATGGCGTGCGGGAAAGAATCCTTAAAGTCGAGCGTCGCACGCTTCCTTCATGGATGGCGAGAAGAGATCCTCCAGATGATACCTCAGTCGCGACCCTGGAACCCATTGACGTGGATTGGCGCATCCCTTTGATCGCTTATCTCAAGCAGCCAGATCCCACTGCCGACAGGAAGATTCGTTTTCTTGCACTAAATTACTTTCTTAGAGGTGACGAGCTGCGACGACGCGGCGAAGATGGCATAGACTTCAGATGTGTTTATGGCTGTGAAGCGAAACAACTTATGCGCGAAGTTCATTCCGGCATTTGCGGTGCTCACCAAGCAGGTCCAAAGATGCGATGGTTGCTCAGACGACATGGATATTTTTGGCCCAAAGTTTTTGTTTCGGACCGGGGGGCAGCTTTCATGGGTGGTGAAGTTGATAAACTGGCAAAGGAATGGGGAATACAGTTCGTCCATTCCAGTCCTTATTATGCTCAGTCTAACGGTCAAGCGGAGGCCAGCAACAAAATCATCATCACTTTGCTGAAGAAAATGTTGGAAGCTAATCCGCGACAATGGCATGAGACCCTTTATGAGACTCTTTGGGCCTACCGCACATCGAAGCGAAATCCCACCGCGACTACACCTTATGCTTTGATGTTTGGCCATGACGCAGTCCTGCCTTTGGAAGTCAACGTCCAATCATTACGAGTCCAAGAGCAACATCATCTCATTGGAGAAGACTACGTTCAGGCTATGTGGCAGGAACATGAAGACCTTAGCGAAAAGCGCTTGGAGGCTTTAGATAGTTTGGTCATGGAAAAGCAACGAGTCGCTCGAGCCTATGACAAGCGAACAAGGGGAAGGAATTACAGTGAAGGAGAGTTGGTTTGGAAAGCAGTTCTCCCGCTTGGCGAAAAATTAGATGGTCGCGGCAAATGGACTCCAAGATGGGAAGGCCCGTACATCATTCATAAAATCTTAGGGAAAGGAGCTTTTCATCTCAAGGACCTGGATGGAGATGTCCATCATAACCCTATCAATGGGAGATACTTGAAGAAATACTTTCTCAATGTCTGGGACTCAGAGGAGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

1151

Amino Acids

131.17

Weight (kDa)

5.61

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 291 - 406 3.9e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 470 - 564 7.8e-21 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 499 - 599 4.8e-16 RNase H-like domain found in reverse transcriptase
RVT_3 PF13456 667 - 785 2.5e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 945, 1218
Acc16I TGCGCA 1 cut(s) 144
Acc36I ACCTGC 1 cut(s) 2690
AccB7I CCANNNNNTGG 1 cut(s) 784
AccBSI CCGCTC 1 cut(s) 1487
AccI GTMKAC 3 cut(s) 630, 945, 975
AclI AACGTT 1 cut(s) 722
AclWI GGATC 3 cut(s) 1322, 2438, 2525
AcoI YGGCCR 3 cut(s) 927, 2055, 3022
AcsI RAATTY 4 cut(s) 644, 1035, 1793, 2326
AcuI CTGAAG 5 cut(s) 180, 261, 1332, 2608, 2924
AcyI GRCGYC 2 cut(s) 942, 1215
AfeI AGCGCT 2 cut(s) 595, 3149
AflIII ACRYGT 2 cut(s) 966, 1683
AgsI TTSAA 5 cut(s) 16, 649, 1381, 1571, 3415
AjiI CACGTC 1 cut(s) 2488
AjnI CCWGG 5 cut(s) 682, 1081, 1230, 2472, 3369
AjuI GAANNNNNNNTTGG 2 cut(s) 2897, 2929
AluBI AGCT 9 cut(s) 46, 273, 1520, 1639, 2238, 2593, 2777, 2921, 3353
AluI AGCT 9 cut(s) 46, 273, 1520, 1639, 2238, 2593, 2777, 2921, 3353
Alw21I GWGCWC 2 cut(s) 1782, 2692
Alw26I GTCTC 5 cut(s) 230, 909, 1978, 2936, 2948
AlwI GGATC 3 cut(s) 1322, 2438, 2525
AlwNI CAGNNNCTG 3 cut(s) 140, 853, 1520
Ama87I CYCGRG 1 cut(s) 3195
Aor51HI AGCGCT 2 cut(s) 595, 3149
ApeKI GCWGC 9 cut(s) 140, 1077, 1517, 1536, 1904, 2333, 2524, 2593, 2774
ApoI RAATTY 4 cut(s) 644, 1035, 1793, 2326
ArsI GACNNNNNNTTYG 2 cut(s) 783, 815
AseI ATTAAT 1 cut(s) 336
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 6 cut(s) 145, 596, 1957, 2499, 2664, 3150
AsuC2I CCSGG 3 cut(s) 1925, 2054, 2768
AsuHPI GGTGA 6 cut(s) 684, 1022, 1853, 2597, 2684, 2801
AsuNHI GCTAGC 1 cut(s) 2023
AvaI CYCGRG 1 cut(s) 3195
AvaII GGWCC 6 cut(s) 535, 1264, 2281, 2702, 2764, 3367
AxyI CCTNAGG 1 cut(s) 1269
BaeGI GKGCMC 1 cut(s) 993
BaeI ACNNNNGTAYC 2 cut(s) 2448, 2481
BalI TGGCCA 2 cut(s) 929, 3024
BanII GRGCYC 1 cut(s) 1463
BbsI GAAGAC 3 cut(s) 681, 3105, 3138
Bbv12I GWGCWC 2 cut(s) 1782, 2692
BbvCI CCTCAGC 1 cut(s) 1521
BbvI GCAGC 9 cut(s) 127, 1064, 1529, 1548, 1891, 2345, 2536, 2580, 2786
BceAI ACGGC 2 cut(s) 1030, 1746
BcgI CGANNNNNNTGC 2 cut(s) 1525, 1559
BciT130I CCWGG 5 cut(s) 684, 1083, 1232, 2474, 3371
BciVI GTATCC 1 cut(s) 79
BcnI CCSGG 3 cut(s) 1925, 2054, 2768
BcoDI GTCTC 5 cut(s) 230, 909, 1978, 2936, 2948
BfaI CTAG 6 cut(s) 609, 1482, 1724, 2024, 2141, 2303
BfmI CTRYAG 2 cut(s) 576, 1802
BfoI RGCGCY 2 cut(s) 597, 3151
BfuAI ACCTGC 1 cut(s) 2690
BfuI GTATCC 1 cut(s) 79
BglI GCCNNNNNGGC 3 cut(s) 1454, 1767, 2354
BglII AGATCT 1 cut(s) 1191
BmcAI AGTACT 1 cut(s) 2148
Bme1390I CCNGG 8 cut(s) 684, 1083, 1232, 1925, 2054, 2474, 2768, 3371
Bme18I GGWCC 6 cut(s) 535, 1264, 2281, 2702, 2764, 3367
BmeT110I CYCGRG 1 cut(s) 3195
BmgBI CACGTC 1 cut(s) 2488
BmiI GGNNCC 6 cut(s) 536, 537, 1266, 1413, 2250, 2478
BmrFI CCNGG 8 cut(s) 684, 1083, 1232, 1925, 2054, 2474, 2768, 3371
BmrI ACTGGG 1 cut(s) 1316
BmsI GCATC 7 cut(s) 235, 798, 1546, 1713, 2219, 2508, 2700
BmtI GCTAGC 1 cut(s) 2027
BmuI ACTGGG 1 cut(s) 1316
BoxI GACNNNNGTC 1 cut(s) 347
BpiI GAAGAC 3 cut(s) 681, 3105, 3138
BpmI CTGGAG 3 cut(s) 483, 1964, 2433
Bpu10I CCTNAGC 3 cut(s) 1521, 1548, 3137
BpuEI CTTGAG 4 cut(s) 254, 1774, 2504, 3347
BpuMI CCSGG 3 cut(s) 1925, 2054, 2768
Bsa29I ATCGAT 1 cut(s) 2065
BsaBI GATNNNNATC 1 cut(s) 3384
BsaHI GRCGYC 2 cut(s) 942, 1215
BsaI GGTCTC 2 cut(s) 909, 2936
BsaJI CCNNGG 8 cut(s) 294, 1081, 1082, 1231, 1881, 1985, 2472, 2767
BsaWI WCCGGW 1 cut(s) 1039
BsaXI ACNNNNNCTCC 6 cut(s) 2022, 2052, 2081, 2111, 3231, 3261
Bse118I RCCGGY 2 cut(s) 955, 2057
Bse21I CCTNAGG 1 cut(s) 1269
Bse3DI GCAATG 1 cut(s) 1707
Bse8I GATNNNNATC 1 cut(s) 3384
BseBI CCWGG 5 cut(s) 684, 1083, 1232, 2474, 3371
BseCI ATCGAT 1 cut(s) 2065
BseDI CCNNGG 8 cut(s) 294, 1081, 1082, 1231, 1881, 1985, 2472, 2767
BseGI GGATG 8 cut(s) 517, 1360, 1558, 1879, 2176, 2436, 2499, 3379
BseJI GATNNNNATC 1 cut(s) 3384
BseMI GCAATG 1 cut(s) 1707
BseRI GAGGAG 2 cut(s) 488, 1532
BseSI GKGCMC 1 cut(s) 993
BseX3I CGGCCG 1 cut(s) 2055
BseXI GCAGC 9 cut(s) 127, 1064, 1529, 1548, 1891, 2345, 2536, 2580, 2786
Bsh1285I CGRYCG 3 cut(s) 1044, 2058, 2065
BshVI ATCGAT 1 cut(s) 2065
BsiEI CGRYCG 3 cut(s) 1044, 2058, 2065
BsiHKAI GWGCWC 2 cut(s) 1782, 2692
BsiHKCI CYCGRG 1 cut(s) 3195
BsiSI CCGG 8 cut(s) 956, 1040, 1137, 1925, 2054, 2058, 2676, 2767
BslFI GGGAC 5 cut(s) 355, 548, 1250, 1862, 3452
BsmAI GTCTC 5 cut(s) 230, 909, 1978, 2936, 2948
BsmFI GGGAC 5 cut(s) 355, 548, 1250, 1862, 3452
BsmI GAATGC 3 cut(s) 155, 1070, 1138
Bso31I GGTCTC 2 cut(s) 909, 2936
BsoBI CYCGRG 1 cut(s) 3195
Bsp1286I GDGCHC 4 cut(s) 993, 1463, 1782, 2692
Bsp1407I TGTACA 1 cut(s) 2173
Bsp19I CCATGG 1 cut(s) 294
Bsp68I TCGCGA 4 cut(s) 431, 1744, 2319, 2468
BspDI ATCGAT 1 cut(s) 2065
BspLI GGNNCC 6 cut(s) 536, 537, 1266, 1413, 2250, 2478
BspMI ACCTGC 1 cut(s) 2690
BspOI GCTAGC 1 cut(s) 2027
BspPI GGATC 3 cut(s) 1322, 2438, 2525
BspTNI GGTCTC 2 cut(s) 909, 2936
BsrBI CCGCTC 1 cut(s) 1487
BsrDI GCAATG 1 cut(s) 1707
BsrFI RCCGGY 2 cut(s) 955, 2057
BsrGI TGTACA 1 cut(s) 2173
BssAI RCCGGY 2 cut(s) 955, 2057
BssECI CCNNGG 8 cut(s) 294, 1081, 1082, 1231, 1881, 1985, 2472, 2767
BssNI GRCGYC 2 cut(s) 942, 1215
BssT1I CCWWGG 2 cut(s) 294, 1985
Bst2UI CCWGG 5 cut(s) 684, 1083, 1232, 2474, 3371
Bst4CI ACNGT 8 cut(s) 12, 999, 1377, 1806, 1972, 2826, 2864, 3233
Bst6I CTCTTC 5 cut(s) 447, 465, 477, 1351, 2434
BstACI GRCGYC 2 cut(s) 942, 1215
BstAPI GCANNNNNTGC 1 cut(s) 307
BstAUI TGTACA 1 cut(s) 2173
BstDSI CCRYGG 2 cut(s) 294, 1881
BstENI CCTNNNNNAGG 1 cut(s) 1547
BstF5I GGATG 8 cut(s) 517, 1360, 1558, 1879, 2176, 2436, 2499, 3379
BstH2I RGCGCY 2 cut(s) 597, 3151
BstHHI GCGC 6 cut(s) 145, 596, 1957, 2499, 2664, 3150
BstMAI GTCTC 5 cut(s) 230, 909, 1978, 2936, 2948
BstMCI CGRYCG 3 cut(s) 1044, 2058, 2065
BstNI CCWGG 5 cut(s) 684, 1083, 1232, 2474, 3371
BstNSI RCATGY 6 cut(s) 310, 682, 970, 1577, 1668, 1687
BstPAI GACNNNNGTC 1 cut(s) 347
BstSCI CCNGG 8 cut(s) 682, 1081, 1230, 1923, 2052, 2472, 2766, 3369
BstSFI CTRYAG 2 cut(s) 576, 1802
BstSLI GKGCMC 1 cut(s) 993
BstV1I GCAGC 9 cut(s) 127, 1064, 1529, 1548, 1891, 2345, 2536, 2580, 2786
BstV2I GAAGAC 3 cut(s) 681, 3105, 3138
BstX2I RGATCY 3 cut(s) 1191, 2443, 2530
BstXI CCANNNNNNTGG 1 cut(s) 2488
BstYI RGATCY 3 cut(s) 1191, 2443, 2530
BstZI CGGCCG 1 cut(s) 2055
Bsu15I ATCGAT 1 cut(s) 2065
Bsu36I CCTNAGG 1 cut(s) 1269
BsuI GTATCC 1 cut(s) 79
BsuTUI ATCGAT 1 cut(s) 2065
BtgI CCRYGG 2 cut(s) 294, 1881
BtgZI GCGATG 1 cut(s) 2728
BtrI CACGTC 1 cut(s) 2488
BtsCI GGATG 8 cut(s) 517, 1360, 1558, 1879, 2176, 2436, 2499, 3379
BtsI GCAGTG 2 cut(s) 231, 2535
BtsIMutI CAGTG 7 cut(s) 231, 854, 1373, 1473, 2102, 2535, 3238
BtuMI TCGCGA 4 cut(s) 431, 1744, 2319, 2468
BveI ACCTGC 1 cut(s) 2690
CaiI CAGNNNCTG 3 cut(s) 140, 853, 1520
CfoI GCGC 6 cut(s) 145, 596, 1957, 2499, 2664, 3150
Cfr10I RCCGGY 2 cut(s) 955, 2057
Cfr42I CCGCGG 1 cut(s) 1884
ClaI ATCGAT 1 cut(s) 2065
CpoI CGGWCCG 1 cut(s) 2764
CseI GACGC 4 cut(s) 986, 2399, 2610, 3038
CspI CGGWCCG 1 cut(s) 2764
EaeI YGGCCR 3 cut(s) 927, 2055, 3022
EagI CGGCCG 1 cut(s) 2055
Eam1104I CTCTTC 5 cut(s) 447, 465, 477, 1351, 2434
EarI CTCTTC 5 cut(s) 447, 465, 477, 1351, 2434
EclXI CGGCCG 1 cut(s) 2055
Eco130I CCWWGG 2 cut(s) 294, 1985
Eco147I AGGCCT 5 cut(s) 416, 687, 739, 1448, 2139
Eco24I GRGCYC 1 cut(s) 1463
Eco31I GGTCTC 2 cut(s) 909, 2936
Eco32I GATATC 1 cut(s) 1028
Eco47I GGWCC 6 cut(s) 535, 1264, 2281, 2702, 2764, 3367
Eco47III AGCGCT 2 cut(s) 595, 3149
Eco52I CGGCCG 1 cut(s) 2055
Eco57I CTGAAG 5 cut(s) 180, 261, 1332, 2608, 2924
Eco81I CCTNAGG 1 cut(s) 1269
Eco88I CYCGRG 1 cut(s) 3195
EcoNI CCTNNNNNAGG 1 cut(s) 1547
EcoO109I RGGNCCY 4 cut(s) 535, 1264, 2125, 3367
EcoRI GAATTC 2 cut(s) 644, 1793
EcoRII CCWGG 5 cut(s) 682, 1081, 1230, 2472, 3369
EcoRV GATATC 1 cut(s) 1028
EcoT14I CCWWGG 2 cut(s) 294, 1985
EcoT38I GRGCYC 1 cut(s) 1463
ErhI CCWWGG 2 cut(s) 294, 1985
FalI AAGNNNNNCTT 8 cut(s) 113, 145, 248, 280, 641, 673, 3407, 3439
FaqI GGGAC 5 cut(s) 355, 548, 1250, 1862, 3452
FauI CCCGC 6 cut(s) 1015, 1470, 1480, 1888, 2379, 3273
FblI GTMKAC 3 cut(s) 630, 945, 975
FokI GGATG 8 cut(s) 524, 1347, 1565, 1886, 2183, 2443, 2486, 3386
FriOI GRGCYC 1 cut(s) 1463
FspBI CTAG 6 cut(s) 609, 1482, 1724, 2024, 2141, 2303
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 6 cut(s) 144, 595, 1956, 2498, 2663, 3149
GsuI CTGGAG 3 cut(s) 483, 1964, 2433
HaeII RGCGCY 2 cut(s) 597, 3151
HapII CCGG 8 cut(s) 956, 1040, 1137, 1925, 2054, 2058, 2676, 2767
HgaI GACGC 4 cut(s) 986, 2399, 2610, 3038
HhaI GCGC 6 cut(s) 145, 596, 1957, 2499, 2664, 3150
Hin1I GRCGYC 2 cut(s) 942, 1215
Hin6I GCGC 6 cut(s) 143, 594, 1955, 2497, 2662, 3148
HinP1I GCGC 6 cut(s) 143, 594, 1955, 2497, 2662, 3148
HincII GTYRAC 2 cut(s) 631, 3052
HindII GTYRAC 2 cut(s) 631, 3052
HpaII CCGG 8 cut(s) 956, 1040, 1137, 1925, 2054, 2058, 2676, 2767
HphI GGTGA 6 cut(s) 684, 1022, 1853, 2597, 2684, 2801
Hpy166II GTNNAC 7 cut(s) 631, 946, 976, 1204, 1623, 2100, 3052
Hpy8I GTNNAC 7 cut(s) 631, 946, 976, 1204, 1623, 2100, 3052
Hpy99I CGWCG 6 cut(s) 632, 1220, 1332, 2415, 2601, 2604
HpyAV CCTTC 6 cut(s) 376, 520, 2433, 3216, 3230, 3308
HpyCH4III ACNGT 8 cut(s) 12, 999, 1377, 1806, 1972, 2826, 2864, 3233
HpyCH4IV ACGT 7 cut(s) 722, 942, 1215, 2243, 2487, 3054, 3105
HpySE526I ACGT 7 cut(s) 722, 942, 1215, 2243, 2487, 3054, 3105
Hsp92I GRCGYC 2 cut(s) 942, 1215
HspAI GCGC 6 cut(s) 143, 594, 1955, 2497, 2662, 3148
KflI GGGWCCC 1 cut(s) 535
KroI GCCGGC 1 cut(s) 2057
KroNI GCCGGC 1 cut(s) 2059
KspI CCGCGG 1 cut(s) 1884
LmnI GCTCC 4 cut(s) 502, 1139, 2030, 3350
Lsp1109I GCAGC 9 cut(s) 127, 1064, 1529, 1548, 1891, 2345, 2536, 2580, 2786
LweI GCATC 7 cut(s) 235, 798, 1546, 1713, 2219, 2508, 2700
MaeI CTAG 6 cut(s) 609, 1482, 1724, 2024, 2141, 2303
MaeII ACGT 7 cut(s) 722, 942, 1215, 2243, 2487, 3054, 3105
MaeIII GTNAC 3 cut(s) 1293, 2365, 2585
MbiI CCGCTC 1 cut(s) 1487
MflI RGATCY 3 cut(s) 1191, 2443, 2530
MhlI GDGCHC 4 cut(s) 993, 1463, 1782, 2692
MlsI TGGCCA 2 cut(s) 929, 3024
MluNI TGGCCA 2 cut(s) 929, 3024
MlyI GAGTC 7 cut(s) 435, 2183, 2950, 3078, 3198, 3295, 3434
MmeI TCCRAC 4 cut(s) 621, 2067, 2259, 2895
Mox20I TGGCCA 2 cut(s) 929, 3024
MroNI GCCGGC 1 cut(s) 2057
MroXI GAANNNNTTC 1 cut(s) 486
MscI TGGCCA 2 cut(s) 929, 3024
MseI TTAA 5 cut(s) 336, 447, 921, 1499, 2400
MslI CAYNNNNRTG 2 cut(s) 1628, 2486
Msp20I TGGCCA 2 cut(s) 929, 3024
MspA1I CMGCKG 6 cut(s) 329, 503, 1022, 1077, 1520, 1883
MspI CCGG 8 cut(s) 956, 1040, 1137, 1925, 2054, 2058, 2676, 2767
MspR9I CCNGG 8 cut(s) 684, 1083, 1232, 1925, 2054, 2474, 2768, 3371
Mva1269I GAATGC 3 cut(s) 155, 1070, 1138
MvaI CCWGG 5 cut(s) 684, 1083, 1232, 2474, 3371
NaeI GCCGGC 1 cut(s) 2059
NciI CCSGG 3 cut(s) 1925, 2054, 2768
NcoI CCATGG 1 cut(s) 294
NgoMIV GCCGGC 1 cut(s) 2057
NheI GCTAGC 1 cut(s) 2023
NlaIV GGNNCC 6 cut(s) 536, 537, 1266, 1413, 2250, 2478
NmuCI GTSAC 1 cut(s) 2585
NruI TCGCGA 4 cut(s) 431, 1744, 2319, 2468
NsbI TGCGCA 1 cut(s) 144
NspI RCATGY 6 cut(s) 310, 682, 970, 1577, 1668, 1687
PaeI GCATGC 1 cut(s) 1668
PaeR7I CTCGAG 1 cut(s) 3195
PasI CCCWGGG 1 cut(s) 1082
PceI AGGCCT 5 cut(s) 416, 687, 739, 1448, 2139
PciI ACATGT 2 cut(s) 966, 1683
PcsI WCGNNNNNNNCGW 1 cut(s) 3193
PctI GAATGC 3 cut(s) 155, 1070, 1138
PdiI GCCGGC 1 cut(s) 2059
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 6 cut(s) 487, 1236, 1332, 2163, 2394, 2551
PflFI GACNNNGTC 1 cut(s) 3032
PflMI CCANNNNNTGG 1 cut(s) 784
Ple19I CGATCG 1 cut(s) 2065
PleI GAGTC 7 cut(s) 434, 2183, 2950, 3077, 3197, 3295, 3434
PpsI GAGTC 7 cut(s) 434, 2183, 2950, 3077, 3197, 3295, 3434
PpuMI RGGWCCY 3 cut(s) 535, 1264, 3367
PscI ACATGT 2 cut(s) 966, 1683
PshAI GACNNNNGTC 1 cut(s) 347
PshBI ATTAAT 1 cut(s) 336
Psp1406I AACGTT 1 cut(s) 722
Psp5II RGGWCCY 3 cut(s) 535, 1264, 3367
Psp6I CCWGG 5 cut(s) 682, 1081, 1230, 2472, 3369
PspGI CCWGG 5 cut(s) 682, 1081, 1230, 2472, 3369
PspN4I GGNNCC 6 cut(s) 536, 537, 1266, 1413, 2250, 2478
PspPPI RGGWCCY 3 cut(s) 535, 1264, 3367
PspXI VCTCGAGB 1 cut(s) 3195
PstNI CAGNNNCTG 3 cut(s) 140, 853, 1520
PsuI RGATCY 3 cut(s) 1191, 2443, 2530
PsyI GACNNNGTC 1 cut(s) 3032
PvuI CGATCG 1 cut(s) 2065
PvuII CAGCTG 1 cut(s) 1520
RruI TCGCGA 4 cut(s) 431, 1744, 2319, 2468
RseI CAYNNNNRTG 2 cut(s) 1628, 2486
Rsr2I CGGWCCG 1 cut(s) 2764
RsrII CGGWCCG 1 cut(s) 2764
SacII CCGCGG 1 cut(s) 1884
SalI GTCGAC 1 cut(s) 629
SaqAI TTAA 5 cut(s) 336, 447, 921, 1499, 2400
ScaI AGTACT 1 cut(s) 2148
SchI GAGTC 7 cut(s) 435, 2183, 2950, 3078, 3198, 3295, 3434
ScrFI CCNGG 8 cut(s) 684, 1083, 1232, 1925, 2054, 2474, 2768, 3371
SduI GDGCHC 4 cut(s) 993, 1463, 1782, 2692
SfaNI GCATC 7 cut(s) 235, 798, 1546, 1713, 2219, 2508, 2700
SfcI CTRYAG 2 cut(s) 576, 1802
Sfr274I CTCGAG 1 cut(s) 3195
Sfr303I CCGCGG 1 cut(s) 1884
SgrBI CCGCGG 1 cut(s) 1884
SinI GGWCC 6 cut(s) 535, 1264, 2281, 2702, 2764, 3367
SlaI CTCGAG 1 cut(s) 3195
SmiMI CAYNNNNRTG 2 cut(s) 1628, 2486
SmlI CTYRAG 5 cut(s) 233, 1753, 2519, 3195, 3362
SmoI CTYRAG 5 cut(s) 233, 1753, 2519, 3195, 3362
SphI GCATGC 1 cut(s) 1668
SseBI AGGCCT 5 cut(s) 416, 687, 739, 1448, 2139
SspMI CTAG 6 cut(s) 609, 1482, 1724, 2024, 2141, 2303
StuI AGGCCT 5 cut(s) 416, 687, 739, 1448, 2139
StyD4I CCNGG 8 cut(s) 682, 1081, 1230, 1923, 2052, 2472, 2766, 3369
StyI CCWWGG 2 cut(s) 294, 1985
TaaI ACNGT 8 cut(s) 12, 999, 1377, 1806, 1972, 2826, 2864, 3233
TaiI ACGT 7 cut(s) 725, 945, 1218, 2246, 2490, 3057, 3108
TaqI TCGA 8 cut(s) 424, 630, 801, 1434, 2065, 2406, 2978, 3196
TatI WGTACW 4 cut(s) 840, 2146, 2173, 2307
TauI GCSGC 8 cut(s) 203, 313, 506, 757, 1678, 1955, 2607, 3294
TfiI GAWTC 6 cut(s) 487, 1236, 1332, 2163, 2394, 2551
Tru1I TTAA 5 cut(s) 336, 447, 921, 1499, 2400
Tru9I TTAA 5 cut(s) 336, 447, 921, 1499, 2400
TscAI CASTG 7 cut(s) 231, 854, 1380, 1473, 2102, 2542, 3238
TseFI GTSAC 1 cut(s) 2585
TseI GCWGC 9 cut(s) 140, 1077, 1517, 1536, 1904, 2333, 2524, 2593, 2774
Tsp45I GTSAC 1 cut(s) 2585
TspGWI ACGGA 1 cut(s) 1353
TspRI CASTG 7 cut(s) 231, 854, 1380, 1473, 2102, 2542, 3238
Tth111I GACNNNGTC 1 cut(s) 3032
Van91I CCANNNNNTGG 1 cut(s) 784
VpaK11BI GGWCC 6 cut(s) 535, 1264, 2281, 2702, 2764, 3367
VspI ATTAAT 1 cut(s) 336
XagI CCTNNNNNAGG 1 cut(s) 1547
XapI RAATTY 4 cut(s) 644, 1035, 1793, 2326
XbaI TCTAGA 1 cut(s) 2302
XceI RCATGY 6 cut(s) 310, 682, 970, 1577, 1668, 1687
XcmI CCANNNNNNNNNTGG 3 cut(s) 292, 1181, 2712
XhoI CTCGAG 1 cut(s) 3195
XmiI GTMKAC 3 cut(s) 630, 945, 975
XmnI GAANNNNTTC 1 cut(s) 486
XspI CTAG 6 cut(s) 609, 1482, 1724, 2024, 2141, 2303
ZraI GACGTC 2 cut(s) 943, 1216
ZrmI AGTACT 1 cut(s) 2148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.