Rw2G050600

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000160
Physical Location & Seq
Forward (+)
78183718 .. 78188180
4463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G050600.1

Sequence Viewer

Length: 3492 bp
ATGGTTAAACGATTCAACGCCGATATCCCAGCAGATGAACCCAAGCTCAATCTTGATGATGACATGGATGAAACAGAAATGGTGGATACCTCTTGCAATATGGTTTATGTGCTCCCTGCTAAGTATGCCTTACCAGTCGCTGCGCAGGAATGTGTAGAAACTGATGCGATTGGAGAACAGCAGTTGCAGATCACTTCAGCTGCAACAACGCAGGTGAAAGAAGCAGTATTCCTTGAGACTAAAGATGCTAACAATAAGGGTTTCTTCATGAGCTTCACAAGACCCACACCCGCCATGGTGCAACACATGCGACCTTTGTATATCACAGCAGAAATGAATGGAACTAAGGTCAGCAAGATAATGGTAGAGGCCTTCCACTCTTTGTATGAGAGGCTATCCTCATACTTGGTGGAAACACAGGCCTATGATCTAGCCGCGACCTTAGAAATTGTTAATGAAGAGTTCACGGATCAAGAAGACGAGGAAGAAGAGATTCAACTTGCTCCAGCGGCATTGGATGACACACCTCCGAAGGTTAGGGACCCTACTGAAAAGGTCAATCTGGGAACAACTGATGAGCCTATAGAAGTGGCTATCAGCAATTACTTAGAGCCTAGCGAGAAACAGAGGCTCGTCGAATTATTGTTGGAATTCAAGGACTGCTTTGCGGAAAAATATGAGGACATGCCAGGCCTATCATCAGACTTGGTATGCCACCAACTACCAACGTTTCCTGATAAGAGGCCTGTGAAGCAAGAACCACGAAGAATGAACTCGGAAACCCAAGTTCTGGTCAAAGAGGAAGTTGAAAAGATGCACAAGTCAGGCATTATCAAAGTAGCCAAGTACAATCAGTGGCTATCCAATATAGTGCCTGTTCGCAAGAAGAATGGCAAGATGAGGGTTTGTGTAGATTACAGAGACCTTAATTTGGCTACACCTAAAGACGTCTACCCCATGCCGGTTGCTGACATGTTGGTAGACGCAGTTGCAGGGCACGAATTGTTGTCTTTCATGGATGGCACAGCGGGATATCACCAAATTCCGGTCGCGGAGGAAGATAGACACAAGACCGCATTCCGTTGCCCTGGGTTTGCAGGAGTTTTTGAATATGTGGTCATGCCTTTTGGACTGAAGAATGCTGGAGCAACATATCAGAGAGCTATGAACCTGATCTTCCACGACATACTTGGGAAGATCTTAGAGGTTTACATTGATGACGTTGCAGGAGATTTCCTGGGATTCATTGTCCATCAAAGAGGCATTGAGGTCCCTGAGGATAAAGCAAACGCGGTCATCAATGCATCTCCCCCGCGAACGAAGAAAGAGTTACAGCGACTACTGGGTAGGATCAACTTTTTGCGACGATTCATTTCTAACTCTTCAGGTAAAATCCATCCGTTTTCCCCACTGTTGAAGTTGCAAGGACAGAATGAGTTTGTGTGGGAACCTAAACATCAAGAGGCTTTCGACAAAATAAAGGCCTATCTGGCGAGCCCGCCAGTGCTTGTTCCTCCTAGAGCGGGATTTCCATTAAAGTTGTATGTTTCAGCAGCTGAGGCTTCCATTGGCAGCCTCCTCGCTCAGGATGATGCAAATGGTGTTGAACATGCCATCTTTTACCTCAGTAGGACACTCACAGATTGCGAAACAAGGTACACTCCAATGGAAAAGCTGTGTCTTACATTATACTTCTCAGCATGCAAGTTGCGGCATTACATGTTGTCCTTTACTACTGGCATCATTGCTCAAACCGATCTAGTCAAATATATGCTATCACGACCTATCTTGAGAGGCCGTATTGGCAAGTGGGTGCTCGCATTATCAGAATTCTCGCTACAGTATGTGCCACAGAAAGCAGTAAAGGGGCAAGCCATAGCAGACTTTCTGGCACATCACCCTATGCTAGATGTCCCCGCGGTTAGAGATTTAGAGGTCGCTGCCACAACTTTAGATCGCCCGGATTTAGCGTGCTTGCCAGAGTACGCCATGCTTTATCAAGACACAATCTCACTCCAGCCTTGGGTGTTATATTTTGACGGGTCAAGAACAGATACGCTAGCAGGAGCAGGGGTTGTCTTGGAAAACCCGGCCGGCGATCGATTTTCCTACTCTTTCCAGTTGGAGTTCCAGTGTACCAATAACCAAGCAGAATATGAGGCCCTCATTATAGGCCTAGAAGTACTACTGGAAATGGGAATCGGAGATGTACAAATACTTGGCGACTCTCTCTTGGTTATCAATCAGTTGTGCAACGAGTTCAGATGCAATAGCTTCACGTTGGTTCCTTATTGGAACAGGGCATTAGACCTGTTGGACCAGTTTGATAACATACATCTAGAGTACATTCCTCGCGAGCGAAATTTTGCAGCGAACGAGTTGGCCCAACTAGCAACAGGGGTAACATTGAGATATGGTGTGCGGGAGAGAATCCTTAAAGTCGAGCGTCGCACGCTTCCTTCATGGATGGCGAGAAGAGATCCTCCAGATGATACCTTAGTCGCGACCCTGGAACCCATTGACGTGGATTGGCGCATCCCTTTGATCGCTTATCTCAAGCAGCCAGATCCCATTGCCGACAGGAAGATTCGTTTTCTTGCACTAAATTACTTTCTTAGAGGTGACGAGCTGCGACGACGAGGCGAAGATGGCATAGACTTCAGATGTGTTTATGGTCGCGAAGCGAAACAACTCATGCGCGAAGTTCATTCCGGCATTTGCGGTGCTCACCAAGCAGGTCCAAAGATGCGATGGTTGCTCAGACGACATGGATATTTTTGGCCCAAAGTTTTTGTTTCGGACCGGGGGGCAGCTTTCATGGGTGGTGAAGTTGATAAACTGGCAAAGGAATGGGGAATACAGTTCGTCCATTCCAGTCCTTATTATGCTCAGTCTAACGGTCAAGCGGAGGCCAGCAATAAAATCATCATCACTTTGCTGAAGAAAATGTTGGAAGCTAATCCGCGACAATGGCATGAGACCCTTTATGAGACTCTTTGGGCCTACCGCACATCGAAGCGAAATCCCACCGCGACTACACCTTATGCTTTGATGTTTGGCCATGACGCAGTCCTGCCTTTGGAAGTCAACGTCCAATCATTACGAGTCCAAGAGCAACATCATCTCATTGGAGAAGACTACGTTCAGGCTATGTGGCAGGAACATGAAGACCTTAGCGAAAAGCGCTTGGAGGCTTTAGATAATTTGGTCATGGAAAAGCAACGAGTCGCTCGAGCCTATGACAAGCGAACAAGGGGAAGGAATTACAGCGAAGGAGAGTTGGTTTGGAAAGCAGTTCTCCCGCTTGGCGAAAAATTAGATGGTCGCGGCAAATGGACTCCAAGATGGGAAGGCCCGTACATCATTTATAAAATCTTAGGGAAAGGAGCTTTTCATCTCAAGGACCTGGATGGAGATGTCCATCATAACCCTATCAATGGGAGATACTTGAAGAAATACTTTCTCAATATCTGGGACGCGGATGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

1163

Amino Acids

132.59

Weight (kDa)

5.62

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 293 - 408 3e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
RT_RNaseH_2 PF17919 482 - 576 9.5e-21 RNase H-like domain found in reverse transcriptase
RT_RNaseH PF17917 511 - 611 7.7e-16 RNase H-like domain found in reverse transcriptase
RVT_3 PF13456 679 - 797 6e-20 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000160)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g11612 FvH4_2g25851 FvH4_3g22802 FvH4_3g22805 FvH4_3g31361 FvH4_3g31382 FvH4_4g05272 FvH4_4g05273 FvH4_4g14567 FvH4_4g15161 FvH4_5g22540 FvH4_6g13113 FvH4_6g31923 FvH4_6g31924 FvH4_6g33651 FvH4_6g45122 FvH4_7g03662
pyrus_communis pycom01g04240 pycom01g24010 pycom02g18210 pycom02g19420 pycom05g06510 pycom09g15750 pycom09g18940 pycom12g02580 pycom12g03100 pycom12g08310 pycom13g22710 pycom13g28060 pycom16g18340 pycom16g18350 pycom16g24880 pycom16g26240 pycom461g00260 pycom520g00980 pycom520g01320
rosa_chinensis RchiOBHm_Chr1g0320731 RchiOBHm_Chr2g0102771 RchiOBHm_Chr3g0462151 RchiOBHm_Chr5g0038141 RchiOBHm_Chr5g0069371 RchiOBHm_Chr7g0239101
rosa_multiflora Rmu_co8038252.1_g000001 Rmu_co8138412.1_g000001 Rmu_co8331087.1_g000001 Rmu_co8479311.1_g000001 Rmu_sc0000112.1_g000018 Rmu_sc0000346.1_g000012 Rmu_sc0002102.1_g000002 Rmu_sc0003505.1_g000013 Rmu_sc0004249.1_g000007 Rmu_sc0004448.1_g000010 Rmu_sc0004594.1_g000011 Rmu_sc0004915.1_g000010 Rmu_sc0008019.1_g000019 Rmu_sc0008303.1_g000001 Rmu_sc0008355.1_g000005 Rmu_sc0009253.1_g000018 Rmu_sc0010211.1_g000002 Rmu_sc0010252.1_g000009 Rmu_sc0011534.1_g000008 Rmu_sc0014811.1_g000009 Rmu_sc0019475.1_g000001 Rmu_sc0024897.1_g000001 Rmu_sc0024898.1_g000001 Rmu_sc0031791.1_g000001 Rmu_sc0038715.1_g000001
rosa_roxburghii Rroxscaffold_165G00436940 Rroxscaffold_174G00435270 Rroxscaffold_177G00434330 Rroxscaffold_177G00434340 Rroxscaffold_177G00434350 Rroxscaffold_1G00002160 Rroxscaffold_1G00025260 Rroxscaffold_1G00042950 Rroxscaffold_1G00068010 Rroxscaffold_2G00107530 Rroxscaffold_3G00221190 Rroxscaffold_3G00221520 Rroxscaffold_3G00223200 Rroxscaffold_4G00322230 Rroxscaffold_4G00331810 Rroxscaffold_5G00385150 Rroxscaffold_5G00387510 Rroxscaffold_6G00410520 Rroxscaffold_6G00423360 Rroxscaffold_6G00423370 Rroxscaffold_6G00429210 Rroxscaffold_7G00158300 Rroxscaffold_7G00163480 Rroxscaffold_7G00171410 Rroxscaffold_7G00178940 Rroxscaffold_7G00199480 Rroxscaffold_7G00205630 Rroxscaffold_7G00207440
rosa_rugosa Rorug01G0056400 Rorug04G0063900 Rorug05G0251400 Rorug07G0221900
rosa_samantha Rh1DG169400 Rh2AG292900 Rh2DG519200 Rh4DG260600 Rh5AG042600 Rh6BG117500
rosa_wichuraiana Rw0G001900 Rw0G005150 Rw0G006380 Rw0G015140 Rw0G016320 Rw0G019440 Rw0G019840 Rw0G023740 Rw1G001590 Rw1G002180 Rw1G003560 Rw1G004280 Rw1G006110 Rw1G006680 Rw1G007920 Rw1G008480 Rw1G009120 Rw1G010310 Rw1G014900 Rw1G019650 Rw1G022070 Rw1G022170 Rw1G041570 Rw2G002740 Rw2G005760 Rw2G032370 Rw2G046770 Rw2G050600 Rw2G051420 Rw3G015930 Rw3G016280 Rw3G019580 Rw3G028420 Rw4G003430 Rw4G005350 Rw4G007530 Rw4G008150 Rw4G009580 Rw4G010250 Rw4G015800 Rw4G017000 Rw4G017910 Rw4G018510 Rw4G019700 Rw4G031010 Rw5G012650 Rw5G020140 Rw5G022060 Rw5G034420 Rw5G035560 Rw5G043010 Rw5G046750 Rw5G047340 Rw5G049500 Rw6G001390 Rw6G002210 Rw6G004530 Rw6G004770 Rw6G005100 Rw6G005890 Rw6G011860 Rw6G014680 Rw6G019480 Rw6G028970 Rw6G033800 Rw7G013050 Rw7G014800 Rw7G025760 Rw7G025850 Rw7G026730 Rw7G026870 Rw7G036330 Rw7G036430 Rw7G039320 Rw7G039510 Rw7G039560 Rw7G041370 Rw7G042050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3369
AarI CACCTGC 1 cut(s) 202
AatII GACGTC 1 cut(s) 951
Acc16I TGCGCA 1 cut(s) 144
Acc36I ACCTGC 2 cut(s) 202, 2726
AccB7I CCANNNNNTGG 1 cut(s) 790
AccBSI CCGCTC 1 cut(s) 1523
AccI GTMKAC 2 cut(s) 951, 981
AclI AACGTT 1 cut(s) 728
AclWI GGATC 4 cut(s) 477, 1358, 2474, 2561
AcoI YGGCCR 2 cut(s) 2091, 3058
AcsI RAATTY 4 cut(s) 650, 1041, 1829, 2362
AcuI CTGAAG 5 cut(s) 180, 1154, 1368, 2644, 2960
AcyI GRCGYC 1 cut(s) 948
AfaI GTAC 8 cut(s) 848, 1658, 1985, 2137, 2184, 2211, 2345, 3359
AfeI AGCGCT 1 cut(s) 3185
AfiI CCNNNNNNNGG 9 cut(s) 790, 931, 961, 1045, 1523, 1585, 2023, 3079, 3437
AflIII ACRYGT 2 cut(s) 972, 1719
AgsI TTSAA 8 cut(s) 16, 497, 655, 809, 1109, 1417, 1607, 3451
AjiI CACGTC 1 cut(s) 2524
AjnI CCWGG 5 cut(s) 688, 1087, 1236, 2508, 3405
AjuI GAANNNNNNNTTGG 2 cut(s) 2933, 2965
Alw21I GWGCWC 3 cut(s) 114, 1818, 2728
Alw26I GTCTC 4 cut(s) 230, 915, 2972, 2984
AlwI GGATC 4 cut(s) 477, 1358, 2474, 2561
AlwNI CAGNNNCTG 2 cut(s) 140, 1556
Ama87I CYCGRG 1 cut(s) 3231
Aor51HI AGCGCT 1 cut(s) 3185
ApeKI GCWGC 9 cut(s) 140, 200, 1553, 1572, 1940, 2369, 2560, 2629, 2810
ApoI RAATTY 4 cut(s) 650, 1041, 1829, 2362
Asp700I GAANNNNTTC 1 cut(s) 492
AspLEI GCGC 4 cut(s) 145, 2535, 2700, 3186
AsuC2I CCSGG 3 cut(s) 1961, 2090, 2804
AsuHPI GGTGA 6 cut(s) 226, 1028, 1889, 2633, 2720, 2837
AsuNHI GCTAGC 1 cut(s) 2059
AvaI CYCGRG 1 cut(s) 3231
AvaII GGWCC 6 cut(s) 541, 1270, 2317, 2738, 2800, 3403
AxyI CCTNAGG 1 cut(s) 1275
BaeGI GKGCMC 1 cut(s) 999
BaeI ACNNNNGTAYC 2 cut(s) 2484, 2517
BalI TGGCCA 1 cut(s) 3060
BanII GRGCYC 1 cut(s) 1499
BbsI GAAGAC 3 cut(s) 483, 3141, 3174
Bbv12I GWGCWC 3 cut(s) 114, 1818, 2728
BbvCI CCTCAGC 1 cut(s) 1557
BbvI GCAGC 9 cut(s) 127, 187, 1565, 1584, 1927, 2381, 2572, 2616, 2822
BceAI ACGGC 1 cut(s) 1782
BcgI CGANNNNNNTGC 2 cut(s) 1561, 1595
BciT130I CCWGG 5 cut(s) 690, 1089, 1238, 2510, 3407
BciVI GTATCC 1 cut(s) 79
BcnI CCSGG 3 cut(s) 1961, 2090, 2804
BcoDI GTCTC 4 cut(s) 230, 915, 2972, 2984
BfaI CTAG 9 cut(s) 431, 615, 1518, 1760, 1907, 2060, 2177, 2339, 2390
BfmI CTRYAG 2 cut(s) 582, 1838
BfoI RGCGCY 1 cut(s) 3187
BfuAI ACCTGC 2 cut(s) 202, 2726
BfuI GTATCC 1 cut(s) 79
BglI GCCNNNNNGGC 2 cut(s) 1490, 1803
BglII AGATCT 1 cut(s) 1197
BmcAI AGTACT 1 cut(s) 2184
Bme1390I CCNGG 8 cut(s) 690, 1089, 1238, 1961, 2090, 2510, 2804, 3407
Bme18I GGWCC 6 cut(s) 541, 1270, 2317, 2738, 2800, 3403
BmeT110I CYCGRG 1 cut(s) 3231
BmgBI CACGTC 1 cut(s) 2524
BmiI GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2286, 2514
BmrFI CCNGG 8 cut(s) 690, 1089, 1238, 1961, 2090, 2510, 2804, 3407
BmrI ACTGGG 1 cut(s) 1352
BmsI GCATC 9 cut(s) 154, 235, 804, 1313, 1582, 1749, 2255, 2544, 2736
BmtI GCTAGC 1 cut(s) 2063
BmuI ACTGGG 1 cut(s) 1352
BpiI GAAGAC 3 cut(s) 483, 3141, 3174
BpmI CTGGAG 4 cut(s) 489, 1164, 2000, 2469
Bpu10I CCTNAGC 3 cut(s) 1557, 1584, 3173
BpuEI CTTGAG 4 cut(s) 254, 1810, 2540, 3383
BpuMI CCSGG 3 cut(s) 1961, 2090, 2804
Bsa29I ATCGAT 1 cut(s) 2101
BsaBI GATNNNNATC 1 cut(s) 3420
BsaHI GRCGYC 1 cut(s) 948
BsaI GGTCTC 2 cut(s) 915, 2972
BsaJI CCNNGG 8 cut(s) 294, 1087, 1088, 1237, 1917, 2021, 2508, 2803
BsaWI WCCGGW 1 cut(s) 1045
BsaXI ACNNNNNCTCC 6 cut(s) 2058, 2088, 2117, 2147, 3267, 3297
Bsc4I CCNNNNNNNGG 9 cut(s) 790, 931, 961, 1045, 1523, 1585, 2023, 3079, 3437
Bse118I RCCGGY 2 cut(s) 961, 2093
Bse21I CCTNAGG 1 cut(s) 1275
Bse3DI GCAATG 2 cut(s) 1743, 2571
Bse8I GATNNNNATC 1 cut(s) 3420
BseBI CCWGG 5 cut(s) 690, 1089, 1238, 2510, 3407
BseCI ATCGAT 1 cut(s) 2101
BseDI CCNNGG 8 cut(s) 294, 1087, 1088, 1237, 1917, 2021, 2508, 2803
BseGI GGATG 9 cut(s) 73, 523, 1024, 1396, 1594, 2472, 2535, 3415, 3487
BseJI GATNNNNATC 1 cut(s) 3420
BseLI CCNNNNNNNGG 9 cut(s) 790, 931, 961, 1045, 1523, 1585, 2023, 3079, 3437
BseMI GCAATG 2 cut(s) 1743, 2571
BseMII CTCAG 7 cut(s) 1266, 1548, 1598, 1639, 1710, 2773, 2903
BseRI GAGGAG 1 cut(s) 1568
BseSI GKGCMC 1 cut(s) 999
BseX3I CGGCCG 1 cut(s) 2091
BseXI GCAGC 9 cut(s) 127, 187, 1565, 1584, 1927, 2381, 2572, 2616, 2822
BseYI CCCAGC 1 cut(s) 28
Bsh1285I CGRYCG 3 cut(s) 1050, 2094, 2101
BshVI ATCGAT 1 cut(s) 2101
BsiEI CGRYCG 3 cut(s) 1050, 2094, 2101
BsiHKAI GWGCWC 3 cut(s) 114, 1818, 2728
BsiHKCI CYCGRG 1 cut(s) 3231
BsiSI CCGG 7 cut(s) 962, 1046, 1961, 2090, 2094, 2712, 2803
BslFI GGGAC 4 cut(s) 554, 1256, 1898, 3488
BslI CCNNNNNNNGG 9 cut(s) 790, 931, 961, 1045, 1523, 1585, 2023, 3079, 3437
BsmAI GTCTC 4 cut(s) 230, 915, 2972, 2984
BsmFI GGGAC 4 cut(s) 554, 1256, 1898, 3488
BsmI GAATGC 2 cut(s) 1076, 1144
Bso31I GGTCTC 2 cut(s) 915, 2972
BsoBI CYCGRG 1 cut(s) 3231
Bsp1286I GDGCHC 5 cut(s) 114, 999, 1499, 1818, 2728
Bsp1407I TGTACA 1 cut(s) 2209
Bsp19I CCATGG 1 cut(s) 294
Bsp68I TCGCGA 3 cut(s) 2355, 2504, 2679
BspCNI CTCAG 7 cut(s) 1267, 1549, 1597, 1638, 1709, 2772, 2902
BspDI ATCGAT 1 cut(s) 2101
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2286, 2514
BspMI ACCTGC 2 cut(s) 202, 2726
BspOI GCTAGC 1 cut(s) 2063
BspPI GGATC 4 cut(s) 477, 1358, 2474, 2561
BspTNI GGTCTC 2 cut(s) 915, 2972
BsrBI CCGCTC 1 cut(s) 1523
BsrDI GCAATG 2 cut(s) 1743, 2571
BsrFI RCCGGY 2 cut(s) 961, 2093
BsrGI TGTACA 1 cut(s) 2209
BssAI RCCGGY 2 cut(s) 961, 2093
BssECI CCNNGG 8 cut(s) 294, 1087, 1088, 1237, 1917, 2021, 2508, 2803
BssNI GRCGYC 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 294, 2021
Bst2UI CCWGG 5 cut(s) 690, 1089, 1238, 2510, 3407
Bst4CI ACNGT 4 cut(s) 1413, 1842, 2862, 2900
Bst6I CTCTTC 4 cut(s) 453, 483, 1387, 2470
BstACI GRCGYC 1 cut(s) 948
BstAPI GCANNNNNTGC 1 cut(s) 307
BstAUI TGTACA 1 cut(s) 2209
BstDSI CCRYGG 2 cut(s) 294, 1917
BstENI CCTNNNNNAGG 1 cut(s) 1583
BstF5I GGATG 9 cut(s) 73, 523, 1024, 1396, 1594, 2472, 2535, 3415, 3487
BstH2I RGCGCY 1 cut(s) 3187
BstHHI GCGC 4 cut(s) 145, 2535, 2700, 3186
BstMAI GTCTC 4 cut(s) 230, 915, 2972, 2984
BstMCI CGRYCG 3 cut(s) 1050, 2094, 2101
BstNI CCWGG 5 cut(s) 690, 1089, 1238, 2510, 3407
BstNSI RCATGY 6 cut(s) 310, 688, 976, 1613, 1704, 1723
BstSCI CCNGG 8 cut(s) 688, 1087, 1236, 1959, 2088, 2508, 2802, 3405
BstSFI CTRYAG 2 cut(s) 582, 1838
BstSLI GKGCMC 1 cut(s) 999
BstV1I GCAGC 9 cut(s) 127, 187, 1565, 1584, 1927, 2381, 2572, 2616, 2822
BstV2I GAAGAC 3 cut(s) 483, 3141, 3174
BstX2I RGATCY 3 cut(s) 1197, 2479, 2566
BstXI CCANNNNNNTGG 1 cut(s) 2524
BstYI RGATCY 3 cut(s) 1197, 2479, 2566
BstZI CGGCCG 1 cut(s) 2091
Bsu15I ATCGAT 1 cut(s) 2101
Bsu36I CCTNAGG 1 cut(s) 1275
BsuI GTATCC 1 cut(s) 79
BsuTUI ATCGAT 1 cut(s) 2101
BtgI CCRYGG 2 cut(s) 294, 1917
BtgZI GCGATG 1 cut(s) 2764
BtrI CACGTC 1 cut(s) 2524
BtsCI GGATG 9 cut(s) 73, 523, 1024, 1396, 1594, 2472, 2535, 3415, 3487
BtsIMutI CAGTG 4 cut(s) 860, 1409, 1509, 2138
BtuMI TCGCGA 3 cut(s) 2355, 2504, 2679
BveI ACCTGC 2 cut(s) 202, 2726
CaiI CAGNNNCTG 2 cut(s) 140, 1556
CciI TCATGA 1 cut(s) 267
CfoI GCGC 4 cut(s) 145, 2535, 2700, 3186
Cfr10I RCCGGY 2 cut(s) 961, 2093
Cfr42I CCGCGG 1 cut(s) 1920
ClaI ATCGAT 1 cut(s) 2101
CpoI CGGWCCG 1 cut(s) 2800
CseI GACGC 4 cut(s) 992, 2435, 3074, 3485
Csp6I GTAC 8 cut(s) 847, 1657, 1984, 2136, 2183, 2210, 2344, 3358
CspI CGGWCCG 1 cut(s) 2800
CviQI GTAC 8 cut(s) 847, 1657, 1984, 2136, 2183, 2210, 2344, 3358
EaeI YGGCCR 2 cut(s) 2091, 3058
EagI CGGCCG 1 cut(s) 2091
Eam1104I CTCTTC 4 cut(s) 453, 483, 1387, 2470
EarI CTCTTC 4 cut(s) 453, 483, 1387, 2470
EclXI CGGCCG 1 cut(s) 2091
Eco130I CCWWGG 2 cut(s) 294, 2021
Eco147I AGGCCT 6 cut(s) 371, 422, 693, 745, 1484, 2175
Eco24I GRGCYC 1 cut(s) 1499
Eco31I GGTCTC 2 cut(s) 915, 2972
Eco32I GATATC 2 cut(s) 25, 1034
Eco47I GGWCC 6 cut(s) 541, 1270, 2317, 2738, 2800, 3403
Eco47III AGCGCT 1 cut(s) 3185
Eco52I CGGCCG 1 cut(s) 2091
Eco57I CTGAAG 5 cut(s) 180, 1154, 1368, 2644, 2960
Eco81I CCTNAGG 1 cut(s) 1275
Eco88I CYCGRG 1 cut(s) 3231
EcoNI CCTNNNNNAGG 1 cut(s) 1583
EcoO109I RGGNCCY 4 cut(s) 541, 1270, 2161, 3403
EcoRI GAATTC 2 cut(s) 650, 1829
EcoRII CCWGG 5 cut(s) 688, 1087, 1236, 2508, 3405
EcoRV GATATC 2 cut(s) 25, 1034
EcoT14I CCWWGG 2 cut(s) 294, 2021
EcoT22I ATGCAT 1 cut(s) 1306
EcoT38I GRGCYC 1 cut(s) 1499
ErhI CCWWGG 2 cut(s) 294, 2021
FalI AAGNNNNNCTT 8 cut(s) 113, 145, 248, 280, 647, 679, 3443, 3475
FaqI GGGAC 4 cut(s) 554, 1256, 1898, 3488
FauI CCCGC 8 cut(s) 298, 1021, 1320, 1506, 1516, 1924, 2415, 3309
FblI GTMKAC 2 cut(s) 951, 981
FokI GGATG 8 cut(s) 80, 530, 1031, 1383, 1601, 2479, 2522, 3422
FriOI GRGCYC 1 cut(s) 1499
FspBI CTAG 9 cut(s) 431, 615, 1518, 1760, 1907, 2060, 2177, 2339, 2390
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 4 cut(s) 144, 2534, 2699, 3185
GsaI CCCAGC 1 cut(s) 32
GsuI CTGGAG 4 cut(s) 489, 1164, 2000, 2469
HaeII RGCGCY 1 cut(s) 3187
HapII CCGG 7 cut(s) 962, 1046, 1961, 2090, 2094, 2712, 2803
HgaI GACGC 4 cut(s) 992, 2435, 3074, 3485
HhaI GCGC 4 cut(s) 145, 2535, 2700, 3186
Hin1I GRCGYC 1 cut(s) 948
Hin6I GCGC 4 cut(s) 143, 2533, 2698, 3184
HinP1I GCGC 4 cut(s) 143, 2533, 2698, 3184
HincII GTYRAC 1 cut(s) 3088
HindII GTYRAC 1 cut(s) 3088
HpaII CCGG 7 cut(s) 962, 1046, 1961, 2090, 2094, 2712, 2803
HphI GGTGA 6 cut(s) 226, 1028, 1889, 2633, 2720, 2837
Hpy166II GTNNAC 7 cut(s) 465, 952, 982, 1210, 1659, 2136, 3088
Hpy8I GTNNAC 7 cut(s) 465, 952, 982, 1210, 1659, 2136, 3088
Hpy99I CGWCG 5 cut(s) 638, 1368, 2451, 2637, 2640
HpyAV CCTTC 6 cut(s) 382, 526, 2469, 3252, 3266, 3344
HpyCH4III ACNGT 4 cut(s) 1413, 1842, 2862, 2900
HpyCH4IV ACGT 7 cut(s) 728, 948, 1221, 2279, 2523, 3090, 3141
HpySE526I ACGT 7 cut(s) 728, 948, 1221, 2279, 2523, 3090, 3141
Hsp92I GRCGYC 1 cut(s) 948
HspAI GCGC 4 cut(s) 143, 2533, 2698, 3184
KflI GGGWCCC 1 cut(s) 541
KroI GCCGGC 1 cut(s) 2093
KroNI GCCGGC 1 cut(s) 2095
KspI CCGCGG 1 cut(s) 1920
LmnI GCTCC 5 cut(s) 117, 508, 1145, 2066, 3386
Lsp1109I GCAGC 9 cut(s) 127, 187, 1565, 1584, 1927, 2381, 2572, 2616, 2822
LweI GCATC 9 cut(s) 154, 235, 804, 1313, 1582, 1749, 2255, 2544, 2736
MaeI CTAG 9 cut(s) 431, 615, 1518, 1760, 1907, 2060, 2177, 2339, 2390
MaeII ACGT 7 cut(s) 728, 948, 1221, 2279, 2523, 3090, 3141
MaeIII GTNAC 3 cut(s) 1329, 2401, 2621
MbiI CCGCTC 1 cut(s) 1523
MflI RGATCY 3 cut(s) 1197, 2479, 2566
MhlI GDGCHC 5 cut(s) 114, 999, 1499, 1818, 2728
MlsI TGGCCA 1 cut(s) 3060
MluNI TGGCCA 1 cut(s) 3060
MlyI GAGTC 5 cut(s) 2219, 2986, 3114, 3234, 3331
MmeI TCCRAC 4 cut(s) 627, 2103, 2295, 2931
Mox20I TGGCCA 1 cut(s) 3060
Mph1103I ATGCAT 1 cut(s) 1306
MroNI GCCGGC 1 cut(s) 2093
MroXI GAANNNNTTC 1 cut(s) 492
MscI TGGCCA 1 cut(s) 3060
MseI TTAA 5 cut(s) 6, 453, 927, 1535, 2436
MslI CAYNNNNRTG 2 cut(s) 1664, 2522
Msp20I TGGCCA 1 cut(s) 3060
MspA1I CMGCKG 5 cut(s) 200, 509, 1028, 1556, 1919
MspI CCGG 7 cut(s) 962, 1046, 1961, 2090, 2094, 2712, 2803
MspR9I CCNGG 8 cut(s) 690, 1089, 1238, 1961, 2090, 2510, 2804, 3407
Mva1269I GAATGC 2 cut(s) 1076, 1144
MvaI CCWGG 5 cut(s) 690, 1089, 1238, 2510, 3407
NaeI GCCGGC 1 cut(s) 2095
NciI CCSGG 3 cut(s) 1961, 2090, 2804
NcoI CCATGG 1 cut(s) 294
NgoMIV GCCGGC 1 cut(s) 2093
NheI GCTAGC 1 cut(s) 2059
NlaIV GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2286, 2514
NmuCI GTSAC 1 cut(s) 2621
NruI TCGCGA 3 cut(s) 2355, 2504, 2679
NsbI TGCGCA 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 1306
NspI RCATGY 6 cut(s) 310, 688, 976, 1613, 1704, 1723
PaeI GCATGC 1 cut(s) 1704
PaeR7I CTCGAG 1 cut(s) 3231
PagI TCATGA 1 cut(s) 267
PaqCI CACCTGC 1 cut(s) 202
PasI CCCWGGG 1 cut(s) 1088
PceI AGGCCT 6 cut(s) 371, 422, 693, 745, 1484, 2175
PciI ACATGT 2 cut(s) 972, 1719
PcsI WCGNNNNNNNCGW 1 cut(s) 3229
PctI GAATGC 2 cut(s) 1076, 1144
PdiI GCCGGC 1 cut(s) 2095
PdmI GAANNNNTTC 1 cut(s) 492
PfeI GAWTC 7 cut(s) 12, 493, 1242, 1368, 2199, 2430, 2587
PflFI GACNNNGTC 1 cut(s) 3068
PflMI CCANNNNNTGG 1 cut(s) 790
Ple19I CGATCG 1 cut(s) 2101
PleI GAGTC 5 cut(s) 2219, 2986, 3113, 3233, 3331
PpsI GAGTC 5 cut(s) 2219, 2986, 3113, 3233, 3331
PpuMI RGGWCCY 3 cut(s) 541, 1270, 3403
PscI ACATGT 2 cut(s) 972, 1719
PsiI TTATAA 1 cut(s) 3369
Psp1406I AACGTT 1 cut(s) 728
Psp5II RGGWCCY 3 cut(s) 541, 1270, 3403
Psp6I CCWGG 5 cut(s) 688, 1087, 1236, 2508, 3405
PspFI CCCAGC 1 cut(s) 28
PspGI CCWGG 5 cut(s) 688, 1087, 1236, 2508, 3405
PspN4I GGNNCC 6 cut(s) 542, 543, 1272, 1449, 2286, 2514
PspPPI RGGWCCY 3 cut(s) 541, 1270, 3403
PspXI VCTCGAGB 1 cut(s) 3231
PstNI CAGNNNCTG 2 cut(s) 140, 1556
PsuI RGATCY 3 cut(s) 1197, 2479, 2566
PsyI GACNNNGTC 1 cut(s) 3068
PvuI CGATCG 1 cut(s) 2101
PvuII CAGCTG 2 cut(s) 200, 1556
RruI TCGCGA 3 cut(s) 2355, 2504, 2679
RsaI GTAC 8 cut(s) 848, 1658, 1985, 2137, 2184, 2211, 2345, 3359
RsaNI GTAC 8 cut(s) 847, 1657, 1984, 2136, 2183, 2210, 2344, 3358
RseI CAYNNNNRTG 2 cut(s) 1664, 2522
Rsr2I CGGWCCG 1 cut(s) 2800
RsrII CGGWCCG 1 cut(s) 2800
SacII CCGCGG 1 cut(s) 1920
SaqAI TTAA 5 cut(s) 6, 453, 927, 1535, 2436
ScaI AGTACT 1 cut(s) 2184
SchI GAGTC 5 cut(s) 2219, 2986, 3114, 3234, 3331
ScrFI CCNGG 8 cut(s) 690, 1089, 1238, 1961, 2090, 2510, 2804, 3407
SduI GDGCHC 5 cut(s) 114, 999, 1499, 1818, 2728
SfaNI GCATC 9 cut(s) 154, 235, 804, 1313, 1582, 1749, 2255, 2544, 2736
SfcI CTRYAG 2 cut(s) 582, 1838
Sfr274I CTCGAG 1 cut(s) 3231
Sfr303I CCGCGG 1 cut(s) 1920
SgrBI CCGCGG 1 cut(s) 1920
SinI GGWCC 6 cut(s) 541, 1270, 2317, 2738, 2800, 3403
SlaI CTCGAG 1 cut(s) 3231
SmiMI CAYNNNNRTG 2 cut(s) 1664, 2522
SmlI CTYRAG 5 cut(s) 233, 1789, 2555, 3231, 3398
SmoI CTYRAG 5 cut(s) 233, 1789, 2555, 3231, 3398
SphI GCATGC 1 cut(s) 1704
SseBI AGGCCT 6 cut(s) 371, 422, 693, 745, 1484, 2175
SspMI CTAG 9 cut(s) 431, 615, 1518, 1760, 1907, 2060, 2177, 2339, 2390
StuI AGGCCT 6 cut(s) 371, 422, 693, 745, 1484, 2175
StyD4I CCNGG 8 cut(s) 688, 1087, 1236, 1959, 2088, 2508, 2802, 3405
StyI CCWWGG 2 cut(s) 294, 2021
TaaI ACNGT 4 cut(s) 1413, 1842, 2862, 2900
TaiI ACGT 7 cut(s) 731, 951, 1224, 2282, 2526, 3093, 3144
TaqI TCGA 6 cut(s) 636, 1470, 2101, 2442, 3014, 3232
TatI WGTACW 4 cut(s) 846, 2182, 2209, 2343
TauI GCSGC 4 cut(s) 437, 512, 1714, 3330
TfiI GAWTC 7 cut(s) 12, 493, 1242, 1368, 2199, 2430, 2587
Tru1I TTAA 5 cut(s) 6, 453, 927, 1535, 2436
Tru9I TTAA 5 cut(s) 6, 453, 927, 1535, 2436
TscAI CASTG 4 cut(s) 860, 1416, 1509, 2138
TseFI GTSAC 1 cut(s) 2621
TseI GCWGC 9 cut(s) 140, 200, 1553, 1572, 1940, 2369, 2560, 2629, 2810
Tsp45I GTSAC 1 cut(s) 2621
TspGWI ACGGA 3 cut(s) 482, 1070, 1389
TspRI CASTG 4 cut(s) 860, 1416, 1509, 2138
Tth111I GACNNNGTC 1 cut(s) 3068
Van91I CCANNNNNTGG 1 cut(s) 790
VpaK11BI GGWCC 6 cut(s) 541, 1270, 2317, 2738, 2800, 3403
XagI CCTNNNNNAGG 1 cut(s) 1583
XapI RAATTY 4 cut(s) 650, 1041, 1829, 2362
XbaI TCTAGA 1 cut(s) 2338
XceI RCATGY 6 cut(s) 310, 688, 976, 1613, 1704, 1723
XcmI CCANNNNNNNNNTGG 3 cut(s) 292, 1187, 2748
XhoI CTCGAG 1 cut(s) 3231
XmiI GTMKAC 2 cut(s) 951, 981
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 9 cut(s) 431, 615, 1518, 1760, 1907, 2060, 2177, 2339, 2390
ZraI GACGTC 1 cut(s) 949
ZrmI AGTACT 1 cut(s) 2184
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.