FvH4_4g06022

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000042
Physical Location & Seq
Forward (+)
5450537 .. 5456171
5635 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g06022.t1

Sequence Viewer

Length: 3282 bp
ATGTTGCTGAAGAGAACTGACGAGTTACACTTGAATGGAATGGATGCTGGTAATAATACAGCTTATAATCAATTAGACGGTGAAGAATTTCAGCAATTGAAACATCTCCATGTCCGCAATGCTGAGTTTAGACATATCATTAATGGGAAGGGCGTTTTTCCCAACTTAAAAAGGTTAGTGGTGAGTGGCATAGATGGTTCGAGATTCTTATTATCATTTTCCATGGCTAGAAGTCTTGTACAACTCACACATCTTGAGATATCCGGATGTCGAACCATGAAAGAGATAGTATCAATAAACGAATCTGATGAAAAGCATATGAAGGACATGTTTTCTAAGCTTCAACATCTGGAGCTAAGAGGGCTGCCAACCCTCACAAGATTCTGCTCAAGAAGTTGTACTGGATATCCATCTTTGGAGATTTTGCAACAACTAGAGAACTACGATGAAGTTGAAGAAATGGACTCTAGAGAAAACATTCAGAATGTTTTGTTTGATGCAAAGGTAGTATTTCCAAGCTTGAAAGGATTGTTCATCGATGGGTTGACCAAGTTAATGACAGTATGGCACAACCAAATTTCTCCAGAGAATTTCAAAAATATAGAGACTGTAGAGATCTATAGGTGTCAGAGTTTGAAAAGTGTCTTTCCATCCTCAATGGTGAGAAATCTTCAGCAGCTTTTGCGTTTATATGTGGGCGATTGCGGAGTGGAGGAAATCATTGAAGATGGAGTACAAACGACATATGAGTTTGTGCTCTCAAAACTAAACTCTGTCAGATTTGAGGATTTGCCCGATCTTAACTGTTTCTATTCTGGAATGCATGCTTCCTGTTGGCCGTCACTTGAAGCTTTGACAGTATATGGTTGCTCTAGAGTAGCATTTTTGGCCCAAGAATGTTCAAGGTTTCAGAGAAAGTATGACTCGATGTATCCAACCCCAACTAACCAAGCTCTCTTTCTAATTAAGAAGGATTCATTCCCAAGCTTGAAAAGAGTGACCATTTCCGGCATGGAGATTTGGGATGGCCCCTTTCCAGTAAATTACTTCAGCAAACTAGAATATTTCCAGGTCGAAAAGAGTAACAATGAGTCGGCTGCTATACTTGAGAAATTCCCTGGTCTCGAAAAATATGGTGAAACAAGTGCTGCTGCTTCTAGTGAAATATCTTCCCATCGGAAAAGCAGAAGTGGGGATATACATGCAGTTGGGGCCCTCTCTCGTTTAAGTCAATTATGGCTTTGTGGCATGTCTCGCCTGATGCATCTAGGGGAAGATAACTCCCAAATGGCAGGTCAAAATTTTCCAAACCTGCAAATTCTAGGGATAGATTGTTGTGACAGCTTACGGAATCTAAGATCATCAGCAATATCCTTCAAGAATCTAACAAAATTGCAAGTAAATGGTTGTTCGGGGTTGGAATACTTGATAACTTACTCCACAGCTAAAAGTCTCAAGCAACTCATAGTCCTATCAGTTGAGAACTGTGTAAGATTGGTAGAAATAGTGGGAAGCAGAGAAGACGATGATAATTTAGGAAATGAGATTGTATTCAGTCGGTTGGAGTATTTGGAACTCTCTAAGCTACCAAGATTGCGGGGCTTTTGCTCTAGAGATTGCATTGTCAAATTCCCTTCTTTGGACAAGTTATTGATAAGCAAACGCCTCAAACTGAAGATCTTTGGTAACGATGAAACGCTACATGTAACCAACGAAGAGGTTGACACAGACGTTGATGCTGATGATGGTGAAACATACGTTGATGCTGATGATGGTGAAACAGGCGTTGATCTTGATGATGGTGAAAAGGAACATATTCAACCCCTTGAAATCACTGCAACTGATCAGGGGGTCATAGCACCAGTGGGAGAAAATCTGGCCATCTTACCAGTAGGCGAAGTCACAACTCCTGTGTTTCCAGTTATTGAGCATACTCTAGTCGCCGAAGTAACTCATACTGAAGCTGTCGAACAAGTATTCTTAGATATTGAGCCTCTAGCCGAAGAAGCAAGGCCTGATGTTCATCGGACTCTTAATGAACCAGAGCAGCATGTTAAGGAACGAAGTGCTCCCTTGGAGCCTTCAGCTGCTGCTAAGGAAATTTTGACACCTACAATTCGTGCACAGGCCCCCGAAAGTTCTCTGAAGTCATCTGTGGAGAAACTGTCCTTAATAGTTCTGTTATTCACATGTTCTCTCTGGATATGTATCTATTTGATATCTTTACGACATTTGACACCTGGAAGTATATGTACTTCACTGCCACTGTGCCACGTAATCAACCCTTTCCATTACCTGACCTCTCGGGAACAGGTCCGGCCCTTTGAGACCACTGCGACTGATCAAGAGTTTATAGCACCAGTGCAAGAAAATAATCTGGCCATCATACCAGTAGGCGAAGTCAGAACTCCTGTGTTCCCAGTCATTGAGCCACCTCCTGTCGTCGAAGTTCCTCATACTGAAGAAAGGGAACAAGCATTCTCAGGCCTGAACCTAGAGCCTGTGGCTGAAGCAGCTAGGCCTGATGCTCATCAGAATTCCAATGCACCAGAGCAGAATCTTGAGGTACAAAGTGATCCCTTTGAGCCTTCAGCTGTTGCTAGAATAACTCCAACTCGTATCATTCCTGCACTGCCCTCTTCAAGTTCTCCAAAGTCATCACGAGCAAAACGTTCCTTGGGAGAGAAACTTCAAGATCTGTCTGCGAAGAAGGCTGCTAGCAATATCGACCAATCTGAGGAGGAGAAGACCAAGCTCAAAGCTCTTATTTCTTCTGCTGAGTTGAGAGACCCCCAAACCAGACAACAGCTCCTGGCAGTGCTGCCCACTCTCCAACTGTCTACTACCAAATGGATTGCTGATCTCGTTGTTCAAGAGCTATCAAAGTTACCACTGGTTCTGGACAAGGAGGAATCATTACGCCAAAAATTGGCTGGTCTTGTAGCTCAGGGCTTCGAGTTGGATGAAAGGAGGCGCATCCTCCTCTCTGAAGGTTCCTTAGCTGAAGAAAACATCCAGAAGGTCCAGGAGCAGAAGGTGGAGCTGCTGACTGTGGAAGAGCAAATCAAGAAGCTTGAGGCGCAAGCAATTTTACTCCGGGCAGAGATCGCCATTGGCGAAAAGAGGTATTCTGGTTTTGAAGCGTCCTTGGATCAGCTAGTTGAGGAACGACAACTCAACCAACTCTCTGTGGATTCAACAAGTGAAGAGCTGCTTAGGACTCGAGCTGTGAGTGACAGCTTATGTACTGCCATCTTTACTCATCTCCGCCAACTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1094

Amino Acids

122.47

Weight (kDa)

5.1

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 33 - 146 2.9e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 159 - 236 3.4e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 338 - 454 4.4e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 453 - 558 2.3e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
Acc36I ACCTGC 2 cut(s) 1283, 1320
AccB7I CCANNNNNTGG 1 cut(s) 2929
AccI GTMKAC 1 cut(s) 2840
AccIII TCCGGA 1 cut(s) 263
AciI CCGC 4 cut(s) 115, 705, 1597, 3268
AclI AACGTT 1 cut(s) 2671
AclWI GGATC 2 cut(s) 2570, 3159
AcoI YGGCCR 3 cut(s) 836, 1878, 2379
AcsI RAATTY 9 cut(s) 86, 576, 589, 1112, 1300, 1317, 1628, 2100, 2536
AfaI GTAC 6 cut(s) 240, 400, 735, 2254, 2568, 3247
AfiI CCNNNNNNNGG 3 cut(s) 1639, 2737, 2929
AflIII ACRYGT 3 cut(s) 327, 1702, 2189
AjnI CCWGG 5 cut(s) 1068, 1117, 2239, 2811, 3024
AjuI GAANNNNNNNTTGG 2 cut(s) 942, 974
Alw21I GWGCWC 3 cut(s) 759, 2071, 2125
Alw26I GTCTC 6 cut(s) 599, 1127, 1257, 1457, 2321, 2781
Alw44I GTGCAC 1 cut(s) 2121
AlwI GGATC 2 cut(s) 2570, 3159
AlwNI CAGNNNCTG 2 cut(s) 2090, 2812
Ama87I CYCGRG 2 cut(s) 2304, 3222
Aor13HI TCCGGA 1 cut(s) 263
ApaI GGGCCC 1 cut(s) 1216
ApaLI GTGCAC 1 cut(s) 2121
ApoI RAATTY 9 cut(s) 86, 576, 589, 1112, 1300, 1317, 1628, 2100, 2536
ArsI GACNNNNNNTTYG 2 cut(s) 1279, 1311
AseI ATTAAT 1 cut(s) 141
Asp700I GAANNNNTTC 3 cut(s) 87, 477, 1815
AspLEI GCGC 2 cut(s) 2976, 3082
AspS9I GGNCC 8 cut(s) 889, 1028, 1212, 1213, 2128, 2314, 2319, 3022
AsuC2I CCSGG 1 cut(s) 3098
AsuHPI GGTGA 7 cut(s) 92, 193, 673, 1148, 1760, 1787, 1814
AsuNHI GCTAGC 1 cut(s) 2717
AvaI CYCGRG 2 cut(s) 2304, 3222
AvaII GGWCC 2 cut(s) 2314, 3022
BaeGI GKGCMC 2 cut(s) 1216, 2125
BalI TGGCCA 2 cut(s) 1880, 2381
BanII GRGCYC 1 cut(s) 1216
BarI GAAGNNNNNNTAC 6 cut(s) 717, 749, 2236, 2239, 2268, 2271
BauI CACGAG 1 cut(s) 2661
BbsI GAAGAC 2 cut(s) 1527, 2753
Bbv12I GWGCWC 3 cut(s) 759, 2071, 2125
BceAI ACGGC 1 cut(s) 823
BciT130I CCWGG 5 cut(s) 1070, 1119, 2241, 2813, 3026
BciVI GTATCC 1 cut(s) 942
BclI TGATCA 2 cut(s) 1843, 2341
BcnI CCSGG 1 cut(s) 3098
BcoDI GTCTC 6 cut(s) 599, 1127, 1257, 1457, 2321, 2781
BfmI CTRYAG 2 cut(s) 609, 619
BfuAI ACCTGC 2 cut(s) 1283, 1320
BfuI GTATCC 1 cut(s) 942
BglII AGATCT 3 cut(s) 615, 1677, 2695
Bme1390I CCNGG 6 cut(s) 1070, 1119, 2241, 2813, 3026, 3098
Bme18I GGWCC 2 cut(s) 2314, 3022
BmeT110I CYCGRG 2 cut(s) 2304, 3222
BmgT120I GGNCC 8 cut(s) 889, 1028, 1212, 1213, 2128, 2314, 2319, 3022
BmiI GGNNCC 6 cut(s) 1030, 1213, 1214, 2079, 2130, 2995
BmrFI CCNGG 6 cut(s) 1070, 1119, 2241, 2813, 3026, 3098
BmrI ACTGGG 1 cut(s) 2414
BmsI GCATC 8 cut(s) 34, 487, 1251, 1273, 1726, 1753, 2515, 2985
BmtI GCTAGC 1 cut(s) 2721
BmuI ACTGGG 1 cut(s) 2414
BpiI GAAGAC 2 cut(s) 1527, 2753
BpmI CTGGAG 2 cut(s) 371, 567
Bpu10I CCTNAGC 4 cut(s) 2094, 2946, 2998, 3215
BpuEI CTTGAG 6 cut(s) 275, 373, 1127, 1439, 2582, 3095
BpuMI CCSGG 1 cut(s) 3098
Bsa29I ATCGAT 1 cut(s) 537
BsaAI YACGTR 1 cut(s) 2275
BsaBI GATNNNNATC 4 cut(s) 409, 2022, 2207, 2529
BsaI GGTCTC 3 cut(s) 1127, 2321, 2781
BsaJI CCNNGG 5 cut(s) 222, 1117, 2073, 2676, 3147
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 4 cut(s) 2150, 2180, 2793, 2823
Bsc4I CCNNNNNNNGG 3 cut(s) 1639, 2737, 2929
Bse1I ACTGG 9 cut(s) 406, 1037, 1862, 1889, 1919, 2360, 2390, 2420, 2898
Bse3DI GCAATG 1 cut(s) 124
Bse8I GATNNNNATC 4 cut(s) 409, 2022, 2207, 2529
BseAI TCCGGA 1 cut(s) 263
BseBI CCWGG 5 cut(s) 1070, 1119, 2241, 2813, 3026
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 5 cut(s) 222, 1117, 2073, 2676, 3147
BseGI GGATG 7 cut(s) 49, 272, 650, 1030, 2968, 2976, 3012
BseJI GATNNNNATC 4 cut(s) 409, 2022, 2207, 2529
BseLI CCNNNNNNNGG 3 cut(s) 1639, 2737, 2929
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 5 cut(s) 114, 2496, 2727, 2769, 2960
BseNI ACTGG 9 cut(s) 406, 1037, 1862, 1889, 1919, 2360, 2390, 2420, 2898
BseRI GAGGAG 3 cut(s) 2753, 2756, 2972
BseSI GKGCMC 2 cut(s) 1216, 2125
BsgI GTGCAG 1 cut(s) 2613
BshVI ATCGAT 1 cut(s) 537
BsiHKAI GWGCWC 3 cut(s) 759, 2071, 2125
BsiHKCI CYCGRG 2 cut(s) 2304, 3222
BsiSI CCGG 4 cut(s) 264, 1008, 2317, 3097
BslI CCNNNNNNNGG 3 cut(s) 1639, 2737, 2929
BsmAI GTCTC 6 cut(s) 599, 1127, 1257, 1457, 2321, 2781
BsmI GAATGC 2 cut(s) 825, 2477
Bso31I GGTCTC 3 cut(s) 1127, 2321, 2781
BsoBI CYCGRG 2 cut(s) 2304, 3222
Bsp120I GGGCCC 1 cut(s) 1212
Bsp1286I GDGCHC 4 cut(s) 759, 1216, 2071, 2125
Bsp13I TCCGGA 1 cut(s) 263
Bsp1407I TGTACA 1 cut(s) 238
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 4 cut(s) 115, 705, 1597, 3268
BspCNI CTCAG 5 cut(s) 115, 2495, 2728, 2770, 2959
BspDI ATCGAT 1 cut(s) 537
BspEI TCCGGA 1 cut(s) 263
BspLI GGNNCC 6 cut(s) 1030, 1213, 1214, 2079, 2130, 2995
BspMI ACCTGC 2 cut(s) 1283, 1320
BspOI GCTAGC 1 cut(s) 2721
BspPI GGATC 2 cut(s) 2570, 3159
BspQI GCTCTTC 2 cut(s) 3051, 3201
BspTNI GGTCTC 3 cut(s) 1127, 2321, 2781
BsrDI GCAATG 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 238
BsrI ACTGG 9 cut(s) 406, 1037, 1862, 1889, 1919, 2360, 2390, 2420, 2898
BssECI CCNNGG 5 cut(s) 222, 1117, 2073, 2676, 3147
BssSI CACGAG 1 cut(s) 2661
BssT1I CCWWGG 4 cut(s) 222, 2073, 2676, 3147
Bst2BI CACGAG 1 cut(s) 2661
Bst2UI CCWGG 5 cut(s) 1070, 1119, 2241, 2813, 3026
Bst6I CTCTTC 5 cut(s) 5, 1710, 2644, 3051, 3201
BstAPI GCANNNNNTGC 1 cut(s) 682
BstAUI TGTACA 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 2275
BstC8I GCNNGC 3 cut(s) 825, 2719, 3084
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 7 cut(s) 49, 272, 650, 1030, 2968, 2976, 3012
BstHHI GCGC 2 cut(s) 2976, 3082
BstMAI GTCTC 6 cut(s) 599, 1127, 1257, 1457, 2321, 2781
BstNI CCWGG 5 cut(s) 1070, 1119, 2241, 2813, 3026
BstNSI RCATGY 7 cut(s) 331, 827, 1205, 1252, 1706, 2054, 2193
BstSCI CCNGG 6 cut(s) 1068, 1117, 2239, 2811, 3024, 3096
BstSFI CTRYAG 2 cut(s) 609, 619
BstSLI GKGCMC 2 cut(s) 1216, 2125
BstV2I GAAGAC 2 cut(s) 1527, 2753
BstX2I RGATCY 3 cut(s) 615, 1677, 2695
BstYI RGATCY 3 cut(s) 615, 1677, 2695
Bsu15I ATCGAT 1 cut(s) 537
BsuI GTATCC 1 cut(s) 942
BsuTUI ATCGAT 1 cut(s) 537
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 7 cut(s) 49, 272, 650, 1030, 2968, 2976, 3012
BtsI GCAGTG 5 cut(s) 1833, 2258, 2331, 2630, 2823
BtsIMutI CAGTG 9 cut(s) 1833, 1869, 2258, 2264, 2331, 2367, 2630, 2823, 2891
BveI ACCTGC 2 cut(s) 1283, 1320
Cac8I GCNNGC 3 cut(s) 825, 2719, 3084
CaiI CAGNNNCTG 2 cut(s) 2090, 2812
CfoI GCGC 2 cut(s) 2976, 3082
Cfr13I GGNCC 8 cut(s) 889, 1028, 1212, 1213, 2128, 2314, 2319, 3022
ClaI ATCGAT 1 cut(s) 537
CseI GACGC 1 cut(s) 3132
Csp6I GTAC 6 cut(s) 239, 399, 734, 2253, 2567, 3246
CviQI GTAC 6 cut(s) 239, 399, 734, 2253, 2567, 3246
EaeI YGGCCR 3 cut(s) 836, 1878, 2379
Eam1104I CTCTTC 5 cut(s) 5, 1710, 2644, 3051, 3201
EarI CTCTTC 5 cut(s) 5, 1710, 2644, 3051, 3201
EciI GGCGGA 1 cut(s) 3257
Eco130I CCWWGG 4 cut(s) 222, 2073, 2676, 3147
Eco147I AGGCCT 3 cut(s) 2014, 2487, 2521
Eco24I GRGCYC 1 cut(s) 1216
Eco31I GGTCTC 3 cut(s) 1127, 2321, 2781
Eco32I GATATC 3 cut(s) 261, 407, 2220
Eco47I GGWCC 2 cut(s) 2314, 3022
Eco88I CYCGRG 2 cut(s) 2304, 3222
EcoO109I RGGNCCY 3 cut(s) 1212, 1213, 2128
EcoRI GAATTC 1 cut(s) 2536
EcoRII CCWGG 5 cut(s) 1068, 1117, 2239, 2811, 3024
EcoRV GATATC 3 cut(s) 261, 407, 2220
EcoT14I CCWWGG 4 cut(s) 222, 2073, 2676, 3147
EcoT22I ATGCAT 2 cut(s) 825, 1266
EcoT38I GRGCYC 1 cut(s) 1216
ErhI CCWWGG 4 cut(s) 222, 2073, 2676, 3147
FalI AAGNNNNNCTT 2 cut(s) 3198, 3230
FauI CCCGC 1 cut(s) 1590
FauNDI CATATG 2 cut(s) 318, 745
FbaI TGATCA 2 cut(s) 1843, 2341
FblI GTMKAC 1 cut(s) 2840
FokI GGATG 7 cut(s) 56, 279, 637, 1037, 2963, 2975, 2999
FriOI GRGCYC 1 cut(s) 1216
GlaI GCGC 2 cut(s) 2975, 3081
GsuI CTGGAG 2 cut(s) 371, 567
HapII CCGG 4 cut(s) 264, 1008, 2317, 3097
HgaI GACGC 1 cut(s) 3132
HhaI GCGC 2 cut(s) 2976, 3082
Hin6I GCGC 2 cut(s) 2974, 3080
HinP1I GCGC 2 cut(s) 2974, 3080
HincII GTYRAC 2 cut(s) 546, 1723
HindII GTYRAC 2 cut(s) 546, 1723
HindIII AAGCTT 5 cut(s) 338, 517, 849, 985, 3071
HpaII CCGG 4 cut(s) 264, 1008, 2317, 3097
HphI GGTGA 7 cut(s) 92, 193, 673, 1148, 1760, 1787, 1814
Hpy166II GTNNAC 4 cut(s) 546, 1723, 2123, 2841
Hpy8I GTNNAC 4 cut(s) 546, 1723, 2123, 2841
Hpy99I CGWCG 1 cut(s) 2447
HpyCH4IV ACGT 4 cut(s) 1731, 1758, 2274, 2671
HpySE526I ACGT 4 cut(s) 1731, 1758, 2274, 2671
HspAI GCGC 2 cut(s) 2974, 3080
Kpn2I TCCGGA 1 cut(s) 263
Ksp22I TGATCA 2 cut(s) 1843, 2341
LguI GCTCTTC 2 cut(s) 3051, 3201
LmnI GCTCC 6 cut(s) 352, 2074, 2077, 2814, 3028, 3040
LweI GCATC 8 cut(s) 34, 487, 1251, 1273, 1726, 1753, 2515, 2985
MaeII ACGT 4 cut(s) 1731, 1758, 2274, 2671
MfeI CAATTG 1 cut(s) 95
MflI RGATCY 3 cut(s) 615, 1677, 2695
MhlI GDGCHC 4 cut(s) 759, 1216, 2071, 2125
MlsI TGGCCA 2 cut(s) 1880, 2381
MluNI TGGCCA 2 cut(s) 1880, 2381
MlyI GAGTC 5 cut(s) 458, 917, 1100, 2023, 3214
MmeI TCCRAC 6 cut(s) 959, 1398, 1542, 2636, 2857, 2940
Mox20I TGGCCA 2 cut(s) 1880, 2381
Mph1103I ATGCAT 2 cut(s) 825, 1266
MroI TCCGGA 1 cut(s) 263
MroXI GAANNNNTTC 3 cut(s) 87, 477, 1815
MscI TGGCCA 2 cut(s) 1880, 2381
MseI TTAA 9 cut(s) 141, 167, 554, 801, 966, 1226, 2034, 2055, 2171
MslI CAYNNNNRTG 2 cut(s) 33, 108
Msp20I TGGCCA 2 cut(s) 1880, 2381
MspA1I CMGCKG 2 cut(s) 2087, 2594
MspI CCGG 4 cut(s) 264, 1008, 2317, 3097
MspR9I CCNGG 6 cut(s) 1070, 1119, 2241, 2813, 3026, 3098
MunI CAATTG 1 cut(s) 95
Mva1269I GAATGC 2 cut(s) 825, 2477
MvaI CCWGG 5 cut(s) 1070, 1119, 2241, 2813, 3026
NciI CCSGG 1 cut(s) 3098
NcoI CCATGG 1 cut(s) 222
NdeI CATATG 2 cut(s) 318, 745
NheI GCTAGC 1 cut(s) 2717
NlaIV GGNNCC 6 cut(s) 1030, 1213, 1214, 2079, 2130, 2995
NmuCI GTSAC 5 cut(s) 840, 997, 1337, 1900, 3233
NsiI ATGCAT 2 cut(s) 825, 1266
NspI RCATGY 7 cut(s) 331, 827, 1205, 1252, 1706, 2054, 2193
PaeI GCATGC 1 cut(s) 827
PaeR7I CTCGAG 1 cut(s) 3222
PceI AGGCCT 3 cut(s) 2014, 2487, 2521
PciI ACATGT 3 cut(s) 327, 1702, 2189
PciSI GCTCTTC 2 cut(s) 3051, 3201
PcsI WCGNNNNNNNCGW 2 cut(s) 2668, 3114
PctI GAATGC 2 cut(s) 825, 2477
PdmI GAANNNNTTC 3 cut(s) 87, 477, 1815
PfeI GAWTC 9 cut(s) 204, 302, 381, 974, 1351, 1381, 2557, 2912, 3194
PflMI CCANNNNNTGG 1 cut(s) 2929
PfoI TCCNGGA 1 cut(s) 3024
PleI GAGTC 5 cut(s) 458, 917, 1099, 2023, 3214
PpsI GAGTC 5 cut(s) 458, 917, 1099, 2023, 3214
Ppu21I YACGTR 1 cut(s) 2275
PscI ACATGT 3 cut(s) 327, 1702, 2189
PshBI ATTAAT 1 cut(s) 141
PsiI TTATAA 1 cut(s) 66
Psp1406I AACGTT 1 cut(s) 2671
Psp6I CCWGG 5 cut(s) 1068, 1117, 2239, 2811, 3024
PspGI CCWGG 5 cut(s) 1068, 1117, 2239, 2811, 3024
PspN4I GGNNCC 6 cut(s) 1030, 1213, 1214, 2079, 2130, 2995
PspOMI GGGCCC 1 cut(s) 1212
PspPI GGNCC 8 cut(s) 889, 1028, 1212, 1213, 2128, 2314, 2319, 3022
PspXI VCTCGAGB 1 cut(s) 3222
PstNI CAGNNNCTG 2 cut(s) 2090, 2812
PsuI RGATCY 3 cut(s) 615, 1677, 2695
PvuII CAGCTG 2 cut(s) 2087, 2594
RsaI GTAC 6 cut(s) 240, 400, 735, 2254, 2568, 3247
RsaNI GTAC 6 cut(s) 239, 399, 734, 2253, 2567, 3246
RseI CAYNNNNRTG 2 cut(s) 33, 108
SapI GCTCTTC 2 cut(s) 3051, 3201
SaqAI TTAA 9 cut(s) 141, 167, 554, 801, 966, 1226, 2034, 2055, 2171
Sau96I GGNCC 8 cut(s) 889, 1028, 1212, 1213, 2128, 2314, 2319, 3022
SchI GAGTC 5 cut(s) 458, 917, 1100, 2023, 3214
ScrFI CCNGG 6 cut(s) 1070, 1119, 2241, 2813, 3026, 3098
SduI GDGCHC 4 cut(s) 759, 1216, 2071, 2125
SfaNI GCATC 8 cut(s) 34, 487, 1251, 1273, 1726, 1753, 2515, 2985
SfcI CTRYAG 2 cut(s) 609, 619
Sfr274I CTCGAG 1 cut(s) 3222
SinI GGWCC 2 cut(s) 2314, 3022
SlaI CTCGAG 1 cut(s) 3222
SmiMI CAYNNNNRTG 2 cut(s) 33, 108
SmlI CTYRAG 7 cut(s) 254, 388, 1106, 1454, 2561, 3074, 3222
SmoI CTYRAG 7 cut(s) 254, 388, 1106, 1454, 2561, 3074, 3222
SphI GCATGC 1 cut(s) 827
SseBI AGGCCT 3 cut(s) 2014, 2487, 2521
SsiI CCGC 4 cut(s) 115, 705, 1597, 3268
SspI AATATT 1 cut(s) 1064
StuI AGGCCT 3 cut(s) 2014, 2487, 2521
StyD4I CCNGG 6 cut(s) 1068, 1117, 2239, 2811, 3024, 3096
StyI CCWWGG 4 cut(s) 222, 2073, 2676, 3147
TaiI ACGT 4 cut(s) 1734, 1761, 2277, 2674
TatI WGTACW 5 cut(s) 238, 398, 733, 2252, 3245
TfiI GAWTC 9 cut(s) 204, 302, 381, 974, 1351, 1381, 2557, 2912, 3194
Tru1I TTAA 9 cut(s) 141, 167, 554, 801, 966, 1226, 2034, 2055, 2171
Tru9I TTAA 9 cut(s) 141, 167, 554, 801, 966, 1226, 2034, 2055, 2171
TscAI CASTG 9 cut(s) 1840, 1869, 2265, 2271, 2338, 2367, 2637, 2823, 2898
TseFI GTSAC 5 cut(s) 840, 997, 1337, 1900, 3233
Tsp45I GTSAC 5 cut(s) 840, 997, 1337, 1900, 3233
TspGWI ACGGA 1 cut(s) 1363
TspRI CASTG 9 cut(s) 1840, 1869, 2265, 2271, 2338, 2367, 2637, 2823, 2898
Van91I CCANNNNNTGG 1 cut(s) 2929
VneI GTGCAC 1 cut(s) 2121
VpaK11BI GGWCC 2 cut(s) 2314, 3022
VspI ATTAAT 1 cut(s) 141
XapI RAATTY 9 cut(s) 86, 576, 589, 1112, 1300, 1317, 1628, 2100, 2536
XbaI TCTAGA 3 cut(s) 467, 872, 1610
XceI RCATGY 7 cut(s) 331, 827, 1205, 1252, 1706, 2054, 2193
XcmI CCANNNNNNNNNTGG 2 cut(s) 1009, 2930
XhoI CTCGAG 1 cut(s) 3222
XmiI GTMKAC 1 cut(s) 2840
XmnI GAANNNNTTC 3 cut(s) 87, 477, 1815
Zsp2I ATGCAT 2 cut(s) 825, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.