Rw4G006420

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000042
Physical Location & Seq
Forward (+)
13364453 .. 13370684
6232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006420.1

Sequence Viewer

Length: 3735 bp
ATGGCTAGAAGTCTTGTACAACTCACACATCTTGAGGTATCTCAATGTCAAACTTTGGAAGAAATAATATCAACAGACGAATATGATGAAAAACATATGAATGACATGTTCTCTAAGCTACAACATCTGGAGCTAAAAGATCTTCCAATCCTCACAAGATTCTGCTCAAGAAGTTATATTGGATCTCCATCTTTAGAGATTTCGCAACTGAAGAACTCCAATGAAATTGAAGAAAGGGACTCAAGAGAAAACCTTAGGAATGTTTTCTTTGACAAAAAGGTAGTATTTCCAAGCCTGAAGAGATTGTTCATCAATGGCTTGACCAAGTTAATGACATTATGGCACAGCCAACTTTCTGCAGAGAGTTTCAAAAATCTAGAGACAGTAGACATTCGCTCGTGTCAGAGTTTGAAAAGTGTTTTTCCATCTTCAATGGTCCATCGTCTACAGCAGTTAATGCATCTGAAAGTGGTCCATTGCGGAGTGGAGGAAATCATTGCAAATGCAGACGGACTACAAACAACACATGAGTTTGTGTTTTCAAAAGTAAACTCTGTTAGATTTGAGGATTTGCCCCATCTTAGGTGTTTCTATCTCGGAATGCATCCTACTAAGTGGCTGTCACTTGAAGATTTGATGGTACTTGGTTGTTGTAAAGTGGCATTTTTGGCCCAAGAATGTTCAAGCTTTGAGAGAAACTTTGAGTCGACGAATCCTACTCCAACTAACCAAGCTCTCTTTCTAATTAAGAAGGATTCATTCCCCAACTTGAAACGAGTAAACGTGCGGGACATGGACATTTGGGATGGTCCAATCCCGGTAAAGTTCTTTAGCAAACTAGAATCTCTTCAAGTCCGCAATCAGCTCTCCAAGTCAGCTGTGCTTGAGAAATTCCTTGATCTCGAAAATCCAAGTGTAACGAGTTTTGCTGCTTCCAATGAAATATCTTTCCATGAAAAAAGCCATAGTGGGGATATAAATGCAGTTGGGGCCCTCCCTCATTTAAGTGAGTTGTGGCTTTATGGCATATCGAAGCTGATGCATCTATGGGAAGATGACACCCAATCAGCAGGCCAAAATATTCCAAACCTGATAGTTCTGGAAATAAGGAGCTGTGATAGCCTAAGGAATCTAAGATCATCAGCGATATCCTTCAAAAATCTGACAAAGCTGGAAGTAATTGGTTGTCCGGAATTAGAATACTTGATAACTTACTCCACAGCTAAAAGTTTCATGCAACTCAAAGTCCTATCAGTTAGTGATTGTGCAAAGATGGTAGAAATAGTGGGAAGCACAGAAGATGACAATTCAGGAAATGAGATTGTATTCAGCCGCTTGGAATATCTTGCACTTTCTAAACTACCAAGATTGCGAGGCTTTTGCTCTGGAGATTGCGTTGTCAGATTCCCATCTTTAGAGATTTCACAACTAAAGAACTACAATGAAACTGAAAAAAGGGACTCAAAAGAAAACCCTGGGAATGCTTTCTTTGACAAAAAGGTAGTATTTCCAAGCTTGAAGAGATTGTTCATCAATGGCTTGACCAAGTTAACAACATTATGGCACAGCCAATTTTCTCCAGAGAGTTTCAAAAATCTAGAGACAGTAGACATTCACTCATGTCAGAGTTTGAAAAGTGTTTTTCCATCTTCAATGGTTTGTCGTCTACAGCAGTTAATGCATCTGAAAGTAGTCAATTGCGGAGTGGAGGAAATCATTGCAAATGCAAACGGACTACAAACAACACATGAGTTTGTGTTCTCAAAAGTAGACTCTGTTAGATTTGACGATTTGCCCCATCTGAGGTGTTTCTATCCCGGAATGCATGCTACTAGGTGGCTGTCACTTGAAGATTTGATAGTATTTGGTTGTTGTAAAGTGGCATTTTTGGCCCAAGAATGTTCAAGCTTTGAGAGAAACTTTGAGTCGACGAATCCTACTCCAACTAACCAAGCTCTCTTTCTAATTAAGAAGGATTCATTCCCCAACTTGAAACGAGTAAACATGCGGGACATGGACATTTGGGATGGTCCAATCCCGGTAGAGTTCTTTAGCAAACTAGAATCTCTTCAAGTCAGTAATCAGCACTCGAAGTCGGTTGTTCTTGAGAAATTCCTTGATCTCGAAAAACCAAGGGTGACCAGTTTTGCTGCTTCCAATGAAATATCTTTCCATGAAGAAAGCCGTAGTGGGGATATAAATGCAGTTGGTGCCCTCCCTCATTTAAGTACATTGCGGCTTTATGGCATGTCAAAGCTGATGCATCTATGGGAAGATAACACCCAATCAGCAGGCCAAAATATTCCAAACCTGATAGTTCTGGAAATAAGGAACTGTGATAGCCTAAGGAATCTACGATCATCAGCGATATCCTTCAAAAATCTGACAAATATGGAAGTAGCTGGTTGTCCGGAATTGGAATACTTGGTCAATTACTCCACAGCTAAAAGTTTGATGCAACTCGAAGTCCTATCAGTTGGGGATTGTGCAAAGATGGTAGAAATAGTGGGAAGCACAGAAGATGACGATTCAGGAAATGAGATTGTATTCGACCGCTTGGAATATCTTGCACTTTCTAAACTACCAAGATTGAGAGGCTTTTGCTCTGGAGATTGCACTGTCAGATTCCCATCTTTGGAAACTTTATCCATAAGCAAACGCCTCAAATTGAAGATGTTTCATAATGATGAACGTGTTTCATCACTGCAGATAACAGACGAAAATGTTGACACAGACGTTGATGTTGATGATGTTGAAACGGAACATGTTCAGCCCCTCGAGATCAATGCGACTGATCAGGGGGTCATAGCACCAATGGAAGAAAATCTGGCCATCATACCAGTAGGCCAAGTCACAACTCCTGTGTTCCCAGTCATCGAGTCTACTCCTGTCGCCGAAGTACCTCATACTGAAGAAGTTGGGGAACAAGCATTCTCAGGCCAGAACCTAAAGCCTCTAGCTGAAGAAGCAAGGCATGACGTTCATCAGACTCCCAATGCACCAGAGCAGCCTGTTGAGGAACGAAGTGATCCCTTAAAGCCTTCAGTTGCTGCTACAGGAACTCCAACACCTACCATTCCTGCGCTGCCTCCACCAAGTTCTCTGAAGTCATCTGGGACAAAACGATCCTTGAGAGAAAAACTTCAAGCTCTGTCTTCCAAAAAGGCTGCTAGCAGTTCCGGCCTGCCCGAGGAGGCGAAGGCCAAGCTCAAAGCTCTTATTGCTACTGCTGACTTGAAAGACCCCCACACCAGACAACAGCTCTTGGCAGTGTTGCCCACTCTCCAACTGTCTACTACCAAATGGATTGCTGATCTCACTGTTCAAGAGCTGTCGAAGTTACCATTGGTCCTGGACAAGGAGGAATCATTATGCCAAGAATTGGCTGCTCTTAAAGCTCAAGGCTCCGAGCTTGATGTAAGGAGTCACAAACTCCTCTCTGAAGTTTCCTTAGCTGAAGAATACAGCCAAAAGATTCAGAAGCAGAAGGTGGAGCTTCTGGCTTTGGAAGAGCAAATCAAGAAGCTTGAGGTGCGAGCAATTTCACTCCGGGATGAGATCGCCCTTGGCGAAAAGAGGTATTCTGGTTTTGAAGCTTCCTTGGATCAGCTAGTTGAGGAACAGCAACTCAACCAACTCTCTGTGGATTCAACAAGCCAAGAGTTGCTTAGGACTCGAGCTGTGAGTGACAGCTTATGTACTGCCATCTTTACTTATCTCTGCCAGCTATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1244

Amino Acids

139.87

Weight (kDa)

5.45

Isoelectric Point (pI)

49.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 65 - 161 8.1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 109 - 216 6.8e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 263 - 379 3.2e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 382 - 476 1.6e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 499 - 568 1.1e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 670 - 787 1.6e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 786 - 890 5.9e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2210
AccB7I CCANNNNNTGG 1 cut(s) 3382
AccI GTMKAC 9 cut(s) 387, 445, 707, 1608, 1666, 1770, 1928, 2882, 3293
AccIII TCCGGA 2 cut(s) 1189, 2410
AciI CCGC 8 cut(s) 480, 787, 856, 1333, 1701, 2008, 2236, 2554
AclWI GGATC 4 cut(s) 190, 3023, 3120, 3612
AcoI YGGCCR 1 cut(s) 2829
AcsI RAATTY 2 cut(s) 890, 2111
AcuI CTGAAG 8 cut(s) 230, 317, 2931, 2982, 3027, 3125, 3462, 3477
AfaI GTAC 5 cut(s) 18, 642, 2230, 2901, 3700
AfiI CCNNNNNNNGG 8 cut(s) 582, 970, 1803, 2191, 2635, 3190, 3358, 3382
AflIII ACRYGT 3 cut(s) 105, 2692, 2764
AjnI CCWGG 2 cut(s) 1474, 3351
AjuI GAANNNNNNNTTGG 6 cut(s) 723, 755, 1944, 1976, 3329, 3361
Alw26I GTCTC 2 cut(s) 374, 1595
AlwI GGATC 4 cut(s) 190, 3023, 3120, 3612
AlwNI CAGNNNCTG 1 cut(s) 3050
Ama87I CYCGRG 3 cut(s) 2777, 3188, 3675
Aor13HI TCCGGA 2 cut(s) 1189, 2410
ApaI GGGCCC 1 cut(s) 994
ApeKI GCWGC 7 cut(s) 929, 2150, 3007, 3050, 3085, 3167, 3386
ApoI RAATTY 2 cut(s) 890, 2111
Asp700I GAANNNNTTC 6 cut(s) 263, 846, 1484, 2067, 2766, 3141
AspLEI GCGC 1 cut(s) 3085
AspS9I GGNCC 9 cut(s) 436, 472, 670, 809, 990, 991, 1891, 2030, 3349
AsuC2I CCSGG 4 cut(s) 818, 1818, 2039, 3551
AsuHPI GGTGA 1 cut(s) 2149
AsuNHI GCTAGC 1 cut(s) 3170
AvaI CYCGRG 3 cut(s) 2777, 3188, 3675
AvaII GGWCC 5 cut(s) 436, 472, 809, 2030, 3349
AxyI CCTNAGG 3 cut(s) 254, 1124, 2345
BaeGI GKGCMC 2 cut(s) 994, 2215
BalI TGGCCA 1 cut(s) 2831
BanI GGYRCC 1 cut(s) 2210
BanII GRGCYC 1 cut(s) 994
BauI CACGAG 1 cut(s) 397
BbsI GAAGAC 1 cut(s) 3147
BbvI GCAGC 7 cut(s) 916, 2137, 3019, 3037, 3072, 3154, 3373
BceAI ACGGC 1 cut(s) 2169
BcgI CGANNNNNNTGC 6 cut(s) 1021, 1055, 1362, 1396, 2768, 2802
BciT130I CCWGG 2 cut(s) 1476, 3353
BclI TGATCA 1 cut(s) 2794
BcnI CCSGG 4 cut(s) 818, 1818, 2039, 3551
BcoDI GTCTC 2 cut(s) 374, 1595
BfaI CTAG 9 cut(s) 6, 377, 839, 1598, 1833, 2060, 2957, 3171, 3611
BfmI CTRYAG 5 cut(s) 357, 446, 1667, 2705, 3054
BglII AGATCT 1 cut(s) 139
BisI GCNGC 9 cut(s) 930, 1333, 2151, 2237, 3008, 3051, 3086, 3168, 3387
BlsI GCNGC 9 cut(s) 931, 1334, 2152, 2238, 3009, 3052, 3087, 3169, 3388
Bme1390I CCNGG 6 cut(s) 818, 1476, 1818, 2039, 3353, 3551
Bme18I GGWCC 5 cut(s) 436, 472, 809, 2030, 3349
BmeT110I CYCGRG 3 cut(s) 2777, 3188, 3675
BmgT120I GGNCC 9 cut(s) 436, 472, 670, 809, 990, 991, 1891, 2030, 3349
BmiI GGNNCC 4 cut(s) 991, 992, 2212, 3406
BmrFI CCNGG 6 cut(s) 818, 1476, 1818, 2039, 3353, 3551
BmrI ACTGGG 1 cut(s) 2864
BmsI GCATC 8 cut(s) 469, 613, 1029, 1051, 1690, 2250, 2272, 2445
BmtI GCTAGC 1 cut(s) 3174
BmuI ACTGGG 1 cut(s) 2864
BpiI GAAGAC 1 cut(s) 3147
BpmI CTGGAG 4 cut(s) 149, 1407, 1563, 2628
Bpu10I CCTNAGC 2 cut(s) 3451, 3668
BpuEI CTTGAG 8 cut(s) 53, 151, 226, 905, 2126, 3151, 3384, 3548
BpuMI CCSGG 4 cut(s) 818, 1818, 2039, 3551
BsaBI GATNNNNATC 3 cut(s) 187, 1408, 2629
BsaJI CCNNGG 6 cut(s) 1474, 1475, 2132, 3189, 3565, 3600
BsaWI WCCGGW 2 cut(s) 1189, 2410
BsaXI ACNNNNNCTCC 4 cut(s) 1380, 1410, 3046, 3076
Bsc4I CCNNNNNNNGG 8 cut(s) 582, 970, 1803, 2191, 2635, 3190, 3358, 3382
Bse1I ACTGG 3 cut(s) 2142, 2840, 2870
Bse21I CCTNAGG 3 cut(s) 254, 1124, 2345
Bse3DI GCAATG 4 cut(s) 475, 495, 1716, 2231
Bse8I GATNNNNATC 3 cut(s) 187, 1408, 2629
BseAI TCCGGA 2 cut(s) 1189, 2410
BseBI CCWGG 2 cut(s) 1476, 3353
BseDI CCNNGG 6 cut(s) 1474, 1475, 2132, 3189, 3565, 3600
BseGI GGATG 4 cut(s) 604, 811, 2032, 3559
BseJI GATNNNNATC 3 cut(s) 187, 1408, 2629
BseLI CCNNNNNNNGG 8 cut(s) 582, 970, 1803, 2191, 2635, 3190, 3358, 3382
BseMI GCAATG 4 cut(s) 475, 495, 1716, 2231
BseMII CTCAG 2 cut(s) 1793, 2949
BseNI ACTGG 3 cut(s) 2142, 2840, 2870
BseRI GAGGAG 2 cut(s) 3206, 3425
BseSI GKGCMC 2 cut(s) 994, 2215
BseXI GCAGC 7 cut(s) 916, 2137, 3019, 3037, 3072, 3154, 3373
Bsh1285I CGRYCG 1 cut(s) 2554
BshNI GGYRCC 1 cut(s) 2210
BsiEI CGRYCG 1 cut(s) 2554
BsiHKCI CYCGRG 3 cut(s) 2777, 3188, 3675
BsiSI CCGG 7 cut(s) 818, 1190, 1818, 2039, 2411, 3180, 3550
BslFI GGGAC 5 cut(s) 251, 803, 1472, 2024, 3130
BslI CCNNNNNNNGG 8 cut(s) 582, 970, 1803, 2191, 2635, 3190, 3358, 3382
BsmAI GTCTC 2 cut(s) 374, 1595
BsmFI GGGAC 5 cut(s) 251, 803, 1472, 2024, 3130
BsmI GAATGC 4 cut(s) 606, 1486, 1827, 2930
BsoBI CYCGRG 3 cut(s) 2777, 3188, 3675
Bsp120I GGGCCC 1 cut(s) 990
Bsp1286I GDGCHC 2 cut(s) 994, 2215
Bsp13I TCCGGA 2 cut(s) 1189, 2410
Bsp1407I TGTACA 1 cut(s) 16
BspACI CCGC 8 cut(s) 480, 787, 856, 1333, 1701, 2008, 2236, 2554
BspCNI CTCAG 2 cut(s) 1794, 2948
BspEI TCCGGA 2 cut(s) 1189, 2410
BspLI GGNNCC 4 cut(s) 991, 992, 2212, 3406
BspMAI CTGCAG 2 cut(s) 361, 2709
BspOI GCTAGC 1 cut(s) 3174
BspPI GGATC 4 cut(s) 190, 3023, 3120, 3612
BspQI GCTCTTC 1 cut(s) 3504
BspT107I GGYRCC 1 cut(s) 2210
BsrDI GCAATG 4 cut(s) 475, 495, 1716, 2231
BsrGI TGTACA 1 cut(s) 16
BsrI ACTGG 3 cut(s) 2142, 2840, 2870
BssECI CCNNGG 6 cut(s) 1474, 1475, 2132, 3189, 3565, 3600
BssSI CACGAG 1 cut(s) 397
BssT1I CCWWGG 3 cut(s) 2132, 3565, 3600
Bst2BI CACGAG 1 cut(s) 397
Bst2UI CCWGG 2 cut(s) 1476, 3353
Bst4CI ACNGT 6 cut(s) 385, 1606, 2336, 2620, 3291, 3322
Bst6I CTCTTC 5 cut(s) 293, 852, 1514, 2073, 3504
BstAPI GCANNNNNTGC 3 cut(s) 457, 1678, 2210
BstAUI TGTACA 1 cut(s) 16
BstC8I GCNNGC 7 cut(s) 1072, 1827, 2293, 3172, 3185, 3537, 3725
BstEII GGTNACC 1 cut(s) 2137
BstENI CCTNNNNNAGG 2 cut(s) 3188, 3356
BstF5I GGATG 4 cut(s) 604, 811, 2032, 3559
BstHHI GCGC 1 cut(s) 3085
BstMAI GTCTC 2 cut(s) 374, 1595
BstMCI CGRYCG 1 cut(s) 2554
BstNI CCWGG 2 cut(s) 1476, 3353
BstNSI RCATGY 5 cut(s) 109, 1829, 2008, 2251, 2768
BstPI GGTNACC 1 cut(s) 2137
BstSCI CCNGG 6 cut(s) 816, 1474, 1816, 2037, 3351, 3549
BstSFI CTRYAG 5 cut(s) 357, 446, 1667, 2705, 3054
BstSLI GKGCMC 2 cut(s) 994, 2215
BstV1I GCAGC 7 cut(s) 916, 2137, 3019, 3037, 3072, 3154, 3373
BstV2I GAAGAC 1 cut(s) 3147
BstX2I RGATCY 2 cut(s) 139, 182
BstYI RGATCY 2 cut(s) 139, 182
Bsu36I CCTNAGG 3 cut(s) 254, 1124, 2345
BtsCI GGATG 4 cut(s) 604, 811, 2032, 3559
BtsI GCAGTG 2 cut(s) 2702, 3276
BtsIMutI CAGTG 4 cut(s) 2616, 2702, 3276, 3318
Cac8I GCNNGC 7 cut(s) 1072, 1827, 2293, 3172, 3185, 3537, 3725
CaiI CAGNNNCTG 1 cut(s) 3050
CfoI GCGC 1 cut(s) 3085
Cfr13I GGNCC 9 cut(s) 436, 472, 670, 809, 990, 991, 1891, 2030, 3349
Csp6I GTAC 5 cut(s) 17, 641, 2229, 2900, 3699
CviQI GTAC 5 cut(s) 17, 641, 2229, 2900, 3699
EaeI YGGCCR 1 cut(s) 2829
Eam1104I CTCTTC 5 cut(s) 293, 852, 1514, 2073, 3504
EarI CTCTTC 5 cut(s) 293, 852, 1514, 2073, 3504
Eco130I CCWWGG 3 cut(s) 2132, 3565, 3600
Eco24I GRGCYC 1 cut(s) 994
Eco32I GATATC 2 cut(s) 1149, 2370
Eco47I GGWCC 5 cut(s) 436, 472, 809, 2030, 3349
Eco57I CTGAAG 8 cut(s) 230, 317, 2931, 2982, 3027, 3125, 3462, 3477
Eco81I CCTNAGG 3 cut(s) 254, 1124, 2345
Eco88I CYCGRG 3 cut(s) 2777, 3188, 3675
Eco91I GGTNACC 1 cut(s) 2137
EcoNI CCTNNNNNAGG 2 cut(s) 3188, 3356
EcoO109I RGGNCCY 2 cut(s) 990, 991
EcoO65I GGTNACC 1 cut(s) 2137
EcoRII CCWGG 2 cut(s) 1474, 3351
EcoRV GATATC 2 cut(s) 1149, 2370
EcoT14I CCWWGG 3 cut(s) 2132, 3565, 3600
EcoT22I ATGCAT 6 cut(s) 462, 606, 1044, 1683, 1827, 2265
EcoT38I GRGCYC 1 cut(s) 994
ErhI CCWWGG 3 cut(s) 2132, 3565, 3600
FalI AAGNNNNNCTT 2 cut(s) 3651, 3683
FaqI GGGAC 5 cut(s) 251, 803, 1472, 2024, 3130
FauI CCCGC 2 cut(s) 780, 2001
FauNDI CATATG 1 cut(s) 96
FbaI TGATCA 1 cut(s) 2794
FblI GTMKAC 9 cut(s) 387, 445, 707, 1608, 1666, 1770, 1928, 2882, 3293
Fnu4HI GCNGC 9 cut(s) 930, 1333, 2151, 2237, 3008, 3051, 3086, 3168, 3387
FokI GGATG 4 cut(s) 591, 818, 2039, 3566
FriOI GRGCYC 1 cut(s) 994
Fsp4HI GCNGC 9 cut(s) 930, 1333, 2151, 2237, 3008, 3051, 3086, 3168, 3387
FspBI CTAG 9 cut(s) 6, 377, 839, 1598, 1833, 2060, 2957, 3171, 3611
GlaI GCGC 1 cut(s) 3084
GluI GCNGC 9 cut(s) 930, 1333, 2151, 2237, 3008, 3051, 3086, 3168, 3387
GsuI CTGGAG 4 cut(s) 149, 1407, 1563, 2628
HapII CCGG 7 cut(s) 818, 1190, 1818, 2039, 2411, 3180, 3550
HhaI GCGC 1 cut(s) 3085
Hin6I GCGC 1 cut(s) 3083
HinP1I GCGC 1 cut(s) 3083
HincII GTYRAC 4 cut(s) 708, 1551, 1929, 2728
HindII GTYRAC 4 cut(s) 708, 1551, 1929, 2728
HindIII AAGCTT 5 cut(s) 685, 1513, 1906, 3524, 3594
HpaI GTTAAC 1 cut(s) 1551
HpaII CCGG 7 cut(s) 818, 1190, 1818, 2039, 2411, 3180, 3550
HphI GGTGA 1 cut(s) 2149
Hpy99I CGWCG 2 cut(s) 712, 1933
HpyAV CCTTC 7 cut(s) 745, 1162, 1966, 2383, 3051, 3193, 3481
HpyCH4III ACNGT 6 cut(s) 385, 1606, 2336, 2620, 3291, 3322
HpyCH4IV ACGT 4 cut(s) 783, 2692, 2736, 2979
HpySE526I ACGT 4 cut(s) 783, 2692, 2736, 2979
HspAI GCGC 1 cut(s) 3083
Kpn2I TCCGGA 2 cut(s) 1189, 2410
Ksp22I TGATCA 1 cut(s) 2794
KspAI GTTAAC 1 cut(s) 1551
LguI GCTCTTC 1 cut(s) 3504
LmnI GCTCC 4 cut(s) 130, 1110, 3410, 3493
Lsp1109I GCAGC 7 cut(s) 916, 2137, 3019, 3037, 3072, 3154, 3373
LweI GCATC 8 cut(s) 469, 613, 1029, 1051, 1690, 2250, 2272, 2445
MaeI CTAG 9 cut(s) 6, 377, 839, 1598, 1833, 2060, 2957, 3171, 3611
MaeII ACGT 4 cut(s) 783, 2692, 2736, 2979
MaeIII GTNAC 8 cut(s) 621, 916, 1842, 2137, 2851, 3339, 3425, 3686
MfeI CAATTG 1 cut(s) 1696
MflI RGATCY 2 cut(s) 139, 182
MhlI GDGCHC 2 cut(s) 994, 2215
MlsI TGGCCA 1 cut(s) 2831
MluNI TGGCCA 1 cut(s) 2831
MlyI GAGTC 9 cut(s) 233, 713, 1454, 1766, 1934, 2888, 2983, 3433, 3667
MmeI TCCRAC 4 cut(s) 746, 1967, 3089, 3310
Mox20I TGGCCA 1 cut(s) 2831
Mph1103I ATGCAT 6 cut(s) 462, 606, 1044, 1683, 1827, 2265
MroI TCCGGA 2 cut(s) 1189, 2410
MroXI GAANNNNTTC 6 cut(s) 263, 846, 1484, 2067, 2766, 3141
MscI TGGCCA 1 cut(s) 2831
MslI CAYNNNNRTG 3 cut(s) 99, 1005, 2685
Msp20I TGGCCA 1 cut(s) 2831
MspA1I CMGCKG 1 cut(s) 878
MspI CCGG 7 cut(s) 818, 1190, 1818, 2039, 2411, 3180, 3550
MspR9I CCNGG 6 cut(s) 818, 1476, 1818, 2039, 3353, 3551
MunI CAATTG 1 cut(s) 1696
Mva1269I GAATGC 4 cut(s) 606, 1486, 1827, 2930
MvaI CCWGG 2 cut(s) 1476, 3353
NciI CCSGG 4 cut(s) 818, 1818, 2039, 3551
NdeI CATATG 1 cut(s) 96
NheI GCTAGC 1 cut(s) 3170
NlaIV GGNNCC 4 cut(s) 991, 992, 2212, 3406
NmuCI GTSAC 6 cut(s) 621, 1842, 2137, 2851, 3425, 3686
NsiI ATGCAT 6 cut(s) 462, 606, 1044, 1683, 1827, 2265
NspI RCATGY 5 cut(s) 109, 1829, 2008, 2251, 2768
PaeI GCATGC 1 cut(s) 1829
PaeR7I CTCGAG 2 cut(s) 2777, 3675
PasI CCCWGGG 1 cut(s) 1475
PciI ACATGT 2 cut(s) 105, 2764
PciSI GCTCTTC 1 cut(s) 3504
PcsI WCGNNNNNNNCGW 1 cut(s) 3567
PctI GAATGC 4 cut(s) 606, 1486, 1827, 2930
PdmI GAANNNNTTC 6 cut(s) 263, 846, 1484, 2067, 2766, 3141
PflMI CCANNNNNTGG 1 cut(s) 3382
PfoI TCCNGGA 3 cut(s) 1816, 3351, 3549
PkrI GCNGC 9 cut(s) 931, 1334, 2152, 2238, 3009, 3052, 3087, 3169, 3388
PleI GAGTC 9 cut(s) 233, 712, 1454, 1766, 1933, 2887, 2983, 3432, 3667
PpsI GAGTC 9 cut(s) 233, 712, 1454, 1766, 1933, 2887, 2983, 3432, 3667
PscI ACATGT 2 cut(s) 105, 2764
Psp6I CCWGG 2 cut(s) 1474, 3351
PspEI GGTNACC 1 cut(s) 2137
PspGI CCWGG 2 cut(s) 1474, 3351
PspN4I GGNNCC 4 cut(s) 991, 992, 2212, 3406
PspOMI GGGCCC 1 cut(s) 990
PspPI GGNCC 9 cut(s) 436, 472, 670, 809, 990, 991, 1891, 2030, 3349
PspXI VCTCGAGB 1 cut(s) 3675
PstI CTGCAG 2 cut(s) 361, 2709
PstNI CAGNNNCTG 1 cut(s) 3050
PsuI RGATCY 2 cut(s) 139, 182
PvuII CAGCTG 1 cut(s) 878
RsaI GTAC 5 cut(s) 18, 642, 2230, 2901, 3700
RsaNI GTAC 5 cut(s) 17, 641, 2229, 2900, 3699
RseI CAYNNNNRTG 3 cut(s) 99, 1005, 2685
SalI GTCGAC 2 cut(s) 706, 1927
SapI GCTCTTC 1 cut(s) 3504
SatI GCNGC 9 cut(s) 930, 1333, 2151, 2237, 3008, 3051, 3086, 3168, 3387
Sau96I GGNCC 9 cut(s) 436, 472, 670, 809, 990, 991, 1891, 2030, 3349
SchI GAGTC 9 cut(s) 233, 713, 1454, 1766, 1934, 2888, 2983, 3433, 3667
ScrFI CCNGG 6 cut(s) 818, 1476, 1818, 2039, 3353, 3551
SduI GDGCHC 2 cut(s) 994, 2215
SfaNI GCATC 8 cut(s) 469, 613, 1029, 1051, 1690, 2250, 2272, 2445
SfcI CTRYAG 5 cut(s) 357, 446, 1667, 2705, 3054
Sfr274I CTCGAG 2 cut(s) 2777, 3675
SinI GGWCC 5 cut(s) 436, 472, 809, 2030, 3349
SlaI CTCGAG 2 cut(s) 2777, 3675
SmiMI CAYNNNNRTG 3 cut(s) 99, 1005, 2685
SphI GCATGC 1 cut(s) 1829
SsiI CCGC 8 cut(s) 480, 787, 856, 1333, 1701, 2008, 2236, 2554
SspI AATATT 2 cut(s) 1081, 2302
SspMI CTAG 9 cut(s) 6, 377, 839, 1598, 1833, 2060, 2957, 3171, 3611
StyD4I CCNGG 6 cut(s) 816, 1474, 1816, 2037, 3351, 3549
StyI CCWWGG 3 cut(s) 2132, 3565, 3600
TaaI ACNGT 6 cut(s) 385, 1606, 2336, 2620, 3291, 3322
TaiI ACGT 4 cut(s) 786, 2695, 2739, 2982
TatI WGTACW 3 cut(s) 16, 2228, 3698
TauI GCSGC 2 cut(s) 1335, 2239
TscAI CASTG 4 cut(s) 2623, 2709, 3276, 3325
TseFI GTSAC 6 cut(s) 621, 1842, 2137, 2851, 3425, 3686
TseI GCWGC 7 cut(s) 929, 2150, 3007, 3050, 3085, 3167, 3386
Tsp45I GTSAC 6 cut(s) 621, 1842, 2137, 2851, 3425, 3686
TspGWI ACGGA 3 cut(s) 525, 1746, 2774
TspRI CASTG 4 cut(s) 2623, 2709, 3276, 3325
Van91I CCANNNNNTGG 1 cut(s) 3382
VpaK11BI GGWCC 5 cut(s) 436, 472, 809, 2030, 3349
XagI CCTNNNNNAGG 2 cut(s) 3188, 3356
XapI RAATTY 2 cut(s) 890, 2111
XbaI TCTAGA 2 cut(s) 376, 1597
XceI RCATGY 5 cut(s) 109, 1829, 2008, 2251, 2768
XhoI CTCGAG 2 cut(s) 2777, 3675
XmiI GTMKAC 9 cut(s) 387, 445, 707, 1608, 1666, 1770, 1928, 2882, 3293
XmnI GAANNNNTTC 6 cut(s) 263, 846, 1484, 2067, 2766, 3141
XspI CTAG 9 cut(s) 6, 377, 839, 1598, 1833, 2060, 2957, 3171, 3611
Zsp2I ATGCAT 6 cut(s) 462, 606, 1044, 1683, 1827, 2265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.