Rh2BG220800

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000042
Physical Location & Seq
Forward (+)
21362284 .. 21369309
7026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG220800.1

Sequence Viewer

Length: 3366 bp
ATGGGTGTCGTTGAAAAAGTGAGGTTGGCCCCTAGACTACCAGAAACTTTGGCAGCACCCTCATATGGGGTTGGTGTTGGTGATGAAGCGACGGTTGCCATTGTAACGAAGAAAAGTGGGCTAACAATTGTAACAAAGAAAAGTGGGCACCAGAATCAACCTGTTGGACAGAACAAAGGCAGCTTTGAAAAACTGAGGAGGCAGACGAAAGAGTTAGTTGCTGCAAGACACAGGGTGCATCACACCATTGCGTTAGAAGAAAGGAAAGGTAAAATAATCGAACCTTATGTCATCAACTGGCTAAATAGTGTGGATGAGGCCATCGAAAGAGTAGACACATTTTTAATGGGTGAGTGTCAAACAAAGACGAAGGGTCTGCAGGGATGGTTGGCTAAGACTAAGACTCTTGTTAATCCGCTGAGCAAGAAAGCAACAGAATTGGAGCAGGTGGTTGTTGAGCTCTATGCAAAAAGGGATTTTCCTTGTGTCTCATACAGTCTTCCCCCACAAGAGGTGCTTAGCATATCTTCCACAAATTACGAGGCATTTGATTCAAGGATTTCAATTCTGGAGAGTATCATGGATGAGCTGAGAAATCCTGATACCAATATGATTTTGGTGTACGGCGTTGGTGGTGTGGGGAAGACCACACTCGTCCGAGAAGTGTTGAGGAAGGCTATAACAGACCACTTGTTTGATGATTTGGTTATGGTACTAAATGTGAAGCAAATTACAGATTTGGGAAGAATTCAACTAGAAATTCTAGTGAAGTTAGGTATGGACGTTCTTGATGATGAGACTATGGCCGAACGAGCCCGTCGTCTATTTGAGAAGATAAAAGACAAGAGGACACTGGTAATTTTAGATGATGTATGGGAAAGAATTGATTTGGAGGCCATCGGACTTCATTGCATGGCAAATTGTAAAATACTGGTTACATCTAGAACTAGAACAGTATTATCTGGTGATATGCGTACGCAAAAAGAATTTGGTCTTGGTGTTTTAGGCGAAGAAGAAAGTTGGAGCTTATTTGAGAAATTTGCTGGTGATGTTGTGAAACAAGATCCTCGTGGACAAAACGTAGCAAACCAAATAGCCAAAAAATGCGGAGGGTTGCCAATTTTAATTGTCACAGTTGCGAGTGCTTTGAAAGGTAGTACTTTAGATGCGTGGGAAGGTGCCTTGAGAAGCTTACAAGCGATTAATTTGTCAGACCAAGTATACTCTGCTGTGGAATTGAGTTATAATCGGTTGGTTGATGAGGAGCTTCAGCAACTGTTCTTGCTTTGTGGAATTACTGCAAGGTCGACAGACTCTGTTGTTCTGGGAGACTTGTTGAAGTATGGTATGGGTTTGGGTTTGTTTAAAAATGTTGATACAGTGGAAGAAGCATATGATGCATTGCGTTCACTGGTTAGAAAACTTAAGGATTCTTGTCTATTACTAGACGGTTATGATGATACACTGATAAGAATGCATGATCTTGTCCGGGATGTTGCTACTAGGATTTCACACAGAGTTCACCATGTCTTATCAGTCACATATGGAGCTGAGTTGAAAGAATGGCCAGATAAGGATTTCTTTGAAAAGTGCACTGTGATAACTTTGCCTTCCTGCAAAATCCCCAGGTTTCCATCAGTGCCTTGGGAATGCCCTGAACTAGAAATGTTTACTTTGAGGGGTACTGACCATGACTCGTTGGAAATCCCGATCAAGTTTTTTGAAGAGATGAAAGTACTTAAAGTGTTGGATTTAACCAATCTATGTATTCGGTCACTACAGCCTCTTAGGTTCCTATACAATCTTCGAACATTGTGTTTGGATCAATGTGTGTTGGAAGACATAGCTATCGTTGGACAACTGAGAAACTTGGAAGTTCTTAGTTTTTTAAATTCCCAATTTAAGCAATTGCCTAGAGAAATAGCGGAATTGATTCATCTACGATTGTTGGATTTGACGGACTGCTCTGAACTTGAAAAGATTCCACCTAATGTTATATCCAGCTTGACAAGACTAGAAGACTTGAGGATGCGAAACAGTTTTAACCAATGGGAGGCTGAGCCAGTGATTGGGGAAAGAAGTAACGCAAGCCTTTCTGAGCTGAAGTACTTGTCTCATCTAACTGCATTGGACATACATATTCCAGATGGCAACATGATTCCAGCGAACATCTTCTTCAGTAAGTTAGAAAGGTACCACATAATAATTGGTGATGAGTGGACATGGCACAATGTGGATGAAACCTTCAATACTTTGAAACTCAAGCTCACTCCAAACAATGACCAATTGCACCAGGATCTAGAAATGCTGCTGAAGAGATCTGACGATTGGCACTTGGATTTGTTGGAGGGTGTTGGTGTTATTGTCAATCAATTTGATAATGAGGATTTTCGACAACTCAAACATCTGCATGTCCAAAACAGTGCTTATGCCGGGTCCAAAGAAATCATTTTGGAGAACCAGGTGGATAGTGTGGTGCAACATTTCATGAATGGGAAGGTTATGTTGCCCAACTTAAGGAGCTTGTCCCTGTATCAATGTGGTGGTTCAAGATTCTTATTATCTTCATCAATGGCTAGATCTTTTCTGCAACTCAAACATCTTCAGATATCCAGATGTGAAACTGTGGAAGAGATAGTATCAACAGCAGATAACATGTCTGGCTCTGAACCAGCTGGCCTGGTTTTCCCAAAATTGGAAATTCTAGAGGTGGTGGACTGTGGCAGGTTAAAGACATTAGGGTTATCTGCAGTGTCCTTCGAGAAGCTTACAACTCTCGAAGTGATTGGCTGTCATGAATTGAAATATTTGACAACTTACTCGGTAGCAGAAAGTTCAATGCAACTTAGAAGAATGGAAGTTAAGAATTGCGAAAGACTGATAGTGATGGTGGTAAGCAATGGAGATCATGGTGCAGAAAATGAAATTAATTTCGGTGGGATGCTATATTTGGAACTTTCTTCTCTACCAAGTCTGCAAGGCTTTTGCTCAGCCCATTGCATTGCCAAATTCCCAGTCTTGGAAACTATAATAGTAAGTGACTGCCCTGTCGAGTTGAAGATTTCTCCTGATGGGGTACTCCGAAGCAAATCAAAACCAGAAAGAGTAGTTACAATAGAAGTCGAGGAGGAGGGTGAAGATGATGATGATGACAACAACCATGATGGAATGTCTGCTACTTTTGTGGACATGCCTCCTGAGCGTTTTGAGCAAGTTGAAACAGTTCAGGTAATAGTTTGCACCTTTGAAGCTTCTCCACTCTTATTTTTCCTTTTCCGTTTAGCAGGCCCAATTTTGATACTTTGTAGAGTCAGGCCAGGGACACAGAAGCAGACCCCTCTGAGCCTCAGAAGGGGGCTGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1121

Amino Acids

126.39

Weight (kDa)

5.42

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 186 - 244 4.1e-07 AAA ATPase domain
NB-ARC PF00931 190 - 349 7.3e-30 NB-ARC domain
LRR_14 PF23598 574 - 731 3.9e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 832 - 958 5.6e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1245
AarI CACCTGC 1 cut(s) 436
AasI GACNNNNNNGTC 1 cut(s) 3047
Acc36I ACCTGC 2 cut(s) 436, 2715
Acc65I GGTACC 1 cut(s) 2193
AccB1I GGYRCC 3 cut(s) 147, 1178, 2193
AccB7I CCANNNNNTGG 1 cut(s) 2069
AccI GTMKAC 3 cut(s) 333, 1221, 1307
AciI CCGC 3 cut(s) 416, 1107, 1925
AclWI GGATC 3 cut(s) 1058, 1830, 2304
AcoI YGGCCR 2 cut(s) 804, 1565
AcsI RAATTY 7 cut(s) 747, 759, 986, 1037, 1891, 2700, 3008
AcuI CTGAAG 5 cut(s) 1253, 2123, 2161, 2333, 2588
AdeI CACNNNGTG 2 cut(s) 235, 2233
AfaI GTAC 9 cut(s) 623, 714, 976, 1159, 1684, 1737, 2108, 2195, 3078
AfiI CCNNNNNNNGG 9 cut(s) 65, 66, 511, 2053, 2069, 2517, 2695, 3019, 3352
AflII CTTAAG 2 cut(s) 1424, 2515
AflIII ACRYGT 1 cut(s) 2655
AhdI GACNNNNNGTC 1 cut(s) 372
AjnI CCWGG 5 cut(s) 1625, 2292, 2460, 2679, 3316
AjuI GAANNNNNNNTTGG 2 cut(s) 978, 1010
Alw21I GWGCWC 2 cut(s) 462, 1595
Alw26I GTCTC 4 cut(s) 493, 791, 1323, 2118
Alw44I GTGCAC 1 cut(s) 1591
AlwI GGATC 3 cut(s) 1058, 1830, 2304
AlwNI CAGNNNCTG 2 cut(s) 1276, 1316
AoxI GGCC 8 cut(s) 27, 318, 804, 894, 1565, 2677, 3286, 3314
ApaLI GTGCAC 1 cut(s) 1591
ApeKI GCWGC 4 cut(s) 53, 180, 221, 2308
ApoI RAATTY 7 cut(s) 747, 759, 986, 1037, 1891, 2700, 3008
ArsI GACNNNNNNTTYG 6 cut(s) 273, 305, 677, 709, 3000, 3032
AseI ATTAAT 2 cut(s) 1203, 2928
Asp700I GAANNNNTTC 4 cut(s) 1932, 1980, 2171, 3222
Asp718I GGTACC 1 cut(s) 2193
AspS9I GGNCC 3 cut(s) 28, 2436, 3287
AsuC2I CCSGG 2 cut(s) 1490, 2434
AsuHPI GGTGA 7 cut(s) 92, 362, 977, 1058, 1514, 2222, 3146
AsuII TTCGAA 1 cut(s) 1807
AvaII GGWCC 1 cut(s) 2436
BaeGI GKGCMC 2 cut(s) 150, 1595
BalI TGGCCA 1 cut(s) 1567
BanI GGYRCC 3 cut(s) 147, 1178, 2193
BanII GRGCYC 2 cut(s) 462, 817
BauI CACGAG 1 cut(s) 1068
BbsI GAAGAC 4 cut(s) 491, 650, 1845, 2025
Bbv12I GWGCWC 2 cut(s) 462, 1595
BbvI GCAGC 4 cut(s) 65, 192, 208, 2295
BccI CCATC 8 cut(s) 329, 378, 905, 1642, 2141, 2881, 3065, 3158
BceAI ACGGC 1 cut(s) 640
BcgI CGANNNNNNTGC 2 cut(s) 358, 392
BciT130I CCWGG 5 cut(s) 1627, 2294, 2462, 2681, 3318
BcnI CCSGG 2 cut(s) 1490, 2434
BcoDI GTCTC 4 cut(s) 493, 791, 1323, 2118
BfmI CTRYAG 3 cut(s) 377, 1778, 2748
BfrI CTTAAG 2 cut(s) 1424, 2515
BfuAI ACCTGC 2 cut(s) 436, 2715
BglII AGATCT 2 cut(s) 2318, 2579
BisI GCNGC 4 cut(s) 54, 181, 222, 2309
BlpI GCTNAGC 4 cut(s) 419, 518, 2058, 2989
BlsI GCNGC 4 cut(s) 55, 182, 223, 2310
BmcAI AGTACT 3 cut(s) 1159, 1737, 2108
Bme1390I CCNGG 7 cut(s) 1490, 1627, 2294, 2434, 2462, 2681, 3318
Bme18I GGWCC 1 cut(s) 2436
BmeRI GACNNNNNGTC 1 cut(s) 372
BmgT120I GGNCC 3 cut(s) 28, 2436, 3287
BmiI GGNNCC 6 cut(s) 30, 149, 1180, 1793, 2195, 2437
BmrFI CCNGG 7 cut(s) 1490, 1627, 2294, 2434, 2462, 2681, 3318
BmrI ACTGGG 1 cut(s) 3008
BmsI GCATC 5 cut(s) 247, 1156, 1387, 2019, 2931
BmuI ACTGGG 1 cut(s) 3008
BpiI GAAGAC 4 cut(s) 491, 650, 1845, 2025
BpmI CTGGAG 1 cut(s) 590
Bpu10I CCTNAGC 1 cut(s) 3198
Bpu1102I GCTNAGC 4 cut(s) 419, 518, 2058, 2989
Bpu14I TTCGAA 1 cut(s) 1807
BpuEI CTTGAG 3 cut(s) 1204, 2044, 2246
BpuMI CCSGG 2 cut(s) 1490, 2434
BsaJI CCNNGG 3 cut(s) 1625, 1643, 3317
Bsc4I CCNNNNNNNGG 9 cut(s) 65, 66, 511, 2053, 2069, 2517, 2695, 3019, 3352
Bse1I ACTGG 6 cut(s) 302, 858, 936, 1416, 2063, 3014
Bse3DI GCAATG 6 cut(s) 246, 907, 1400, 2905, 2995, 3000
BseBI CCWGG 5 cut(s) 1627, 2294, 2462, 2681, 3318
BseDI CCNNGG 3 cut(s) 1625, 1643, 3317
BseGI GGATG 7 cut(s) 319, 389, 589, 1498, 2034, 2242, 2946
BseLI CCNNNNNNNGG 9 cut(s) 65, 66, 511, 2053, 2069, 2517, 2695, 3019, 3352
BseMI GCAATG 6 cut(s) 246, 907, 1400, 2905, 2995, 3000
BseNI ACTGG 6 cut(s) 302, 858, 936, 1416, 2063, 3014
BseRI GAGGAG 4 cut(s) 211, 1277, 3140, 3143
BseSI GKGCMC 2 cut(s) 150, 1595
BseXI GCAGC 4 cut(s) 65, 192, 208, 2295
BsgI GTGCAG 1 cut(s) 2934
BshFI GGCC 8 cut(s) 29, 320, 806, 896, 1567, 2679, 3288, 3316
BshNI GGYRCC 3 cut(s) 147, 1178, 2193
BsiHKAI GWGCWC 2 cut(s) 462, 1595
BsiSI CCGG 2 cut(s) 1489, 2433
BsiWI CGTACG 1 cut(s) 974
BslFI GGGAC 2 cut(s) 2512, 3334
BslI CCNNNNNNNGG 9 cut(s) 65, 66, 511, 2053, 2069, 2517, 2695, 3019, 3352
BsmAI GTCTC 4 cut(s) 493, 791, 1323, 2118
BsmFI GGGAC 2 cut(s) 2512, 3334
BsmI GAATGC 2 cut(s) 1479, 1655
BsnI GGCC 8 cut(s) 29, 320, 806, 896, 1567, 2679, 3288, 3316
Bsp119I TTCGAA 1 cut(s) 1807
Bsp1286I GDGCHC 4 cut(s) 150, 462, 817, 1595
Bsp143I GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
Bsp1720I GCTNAGC 4 cut(s) 419, 518, 2058, 2989
BspACI CCGC 3 cut(s) 416, 1107, 1925
BspANI GGCC 8 cut(s) 29, 320, 806, 896, 1567, 2679, 3288, 3316
BspHI TCATGA 2 cut(s) 2487, 2794
BspLI GGNNCC 6 cut(s) 30, 149, 1180, 1793, 2195, 2437
BspMAI CTGCAG 2 cut(s) 381, 2752
BspMI ACCTGC 2 cut(s) 436, 2715
BspPI GGATC 3 cut(s) 1058, 1830, 2304
BspT104I TTCGAA 1 cut(s) 1807
BspT107I GGYRCC 3 cut(s) 147, 1178, 2193
BspTI CTTAAG 2 cut(s) 1424, 2515
BsrDI GCAATG 6 cut(s) 246, 907, 1400, 2905, 2995, 3000
BsrI ACTGG 6 cut(s) 302, 858, 936, 1416, 2063, 3014
BssECI CCNNGG 3 cut(s) 1625, 1643, 3317
BssMI GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
BssNAI GTATAC 1 cut(s) 1222
BssSI CACGAG 1 cut(s) 1068
BssT1I CCWWGG 1 cut(s) 1643
Bst1107I GTATAC 1 cut(s) 1222
Bst2BI CACGAG 1 cut(s) 1068
Bst2UI CCWGG 5 cut(s) 1627, 2294, 2462, 2681, 3318
Bst6I CTCTTC 3 cut(s) 1719, 2309, 2625
BstAFI CTTAAG 2 cut(s) 1424, 2515
BstAPI GCANNNNNTGC 1 cut(s) 1397
BstBI TTCGAA 1 cut(s) 1807
BstC8I GCNNGC 3 cut(s) 2089, 2677, 3286
BstF5I GGATG 7 cut(s) 319, 389, 589, 1498, 2034, 2242, 2946
BstKTI GATC 8 cut(s) 1066, 1483, 1713, 1825, 2299, 2321, 2582, 2908
BstMAI GTCTC 4 cut(s) 493, 791, 1323, 2118
BstMBI GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
BstMWI GCNNNNNNNGC 5 cut(s) 95, 812, 1397, 3199, 3208
BstNI CCWGG 5 cut(s) 1627, 2294, 2462, 2681, 3318
BstNSI RCATGY 3 cut(s) 2414, 2659, 3193
BstSCI CCNGG 7 cut(s) 1488, 1625, 2292, 2432, 2460, 2679, 3316
BstSFI CTRYAG 3 cut(s) 377, 1778, 2748
BstSLI GKGCMC 2 cut(s) 150, 1595
BstV1I GCAGC 4 cut(s) 65, 192, 208, 2295
BstV2I GAAGAC 4 cut(s) 491, 650, 1845, 2025
BstX2I RGATCY 4 cut(s) 1063, 2296, 2318, 2579
BstYI RGATCY 4 cut(s) 1063, 2296, 2318, 2579
BstZ17I GTATAC 1 cut(s) 1222
BsuRI GGCC 8 cut(s) 29, 320, 806, 896, 1567, 2679, 3288, 3316
BtsCI GGATG 7 cut(s) 319, 389, 589, 1498, 2034, 2242, 2946
BtsI GCAGTG 1 cut(s) 2757
BtsIMutI CAGTG 9 cut(s) 851, 1386, 1409, 1463, 1593, 1644, 2070, 2428, 2757
BveI ACCTGC 2 cut(s) 436, 2715
Cac8I GCNNGC 3 cut(s) 2089, 2677, 3286
CaiI CAGNNNCTG 2 cut(s) 1276, 1316
CciI TCATGA 2 cut(s) 2487, 2794
Cfr13I GGNCC 3 cut(s) 28, 2436, 3287
CsiI ACCWGGT 1 cut(s) 2460
Csp6I GTAC 9 cut(s) 622, 713, 975, 1158, 1683, 1736, 2107, 2194, 3077
CviQI GTAC 9 cut(s) 622, 713, 975, 1158, 1683, 1736, 2107, 2194, 3077
DpnI GATC 8 cut(s) 1065, 1482, 1712, 1824, 2298, 2320, 2581, 2907
DpnII GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
DraI TTTAAA 2 cut(s) 1366, 1890
DraIII CACNNNGTG 2 cut(s) 235, 2233
DrdI GACNNNNNNGTC 1 cut(s) 3047
DriI GACNNNNNGTC 1 cut(s) 372
DseDI GACNNNNNNGTC 1 cut(s) 3047
EaeI YGGCCR 2 cut(s) 804, 1565
Eam1104I CTCTTC 3 cut(s) 1719, 2309, 2625
Eam1105I GACNNNNNGTC 1 cut(s) 372
EarI CTCTTC 3 cut(s) 1719, 2309, 2625
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 1643
Eco24I GRGCYC 2 cut(s) 462, 817
Eco32I GATATC 1 cut(s) 2610
Eco47I GGWCC 1 cut(s) 2436
Eco53kI GAGCTC 1 cut(s) 460
Eco57I CTGAAG 5 cut(s) 1253, 2123, 2161, 2333, 2588
EcoICRI GAGCTC 1 cut(s) 460
EcoRI GAATTC 1 cut(s) 747
EcoRII CCWGG 5 cut(s) 1625, 2292, 2460, 2679, 3316
EcoRV GATATC 1 cut(s) 2610
EcoT14I CCWWGG 1 cut(s) 1643
EcoT22I ATGCAT 2 cut(s) 1402, 1479
EcoT38I GRGCYC 2 cut(s) 462, 817
ErhI CCWWGG 1 cut(s) 1643
FalI AAGNNNNNCTT 4 cut(s) 501, 533, 1565, 1597
FaqI GGGAC 2 cut(s) 2512, 3334
FauNDI CATATG 3 cut(s) 64, 1393, 1543
FblI GTMKAC 3 cut(s) 333, 1221, 1307
Fnu4HI GCNGC 4 cut(s) 54, 181, 222, 2309
FokI GGATG 7 cut(s) 326, 396, 596, 1505, 2041, 2249, 2953
FriOI GRGCYC 2 cut(s) 462, 817
Fsp4HI GCNGC 4 cut(s) 54, 181, 222, 2309
GluI GCNGC 4 cut(s) 54, 181, 222, 2309
GsuI CTGGAG 1 cut(s) 590
HaeIII GGCC 8 cut(s) 29, 320, 806, 896, 1567, 2679, 3288, 3316
HapII CCGG 2 cut(s) 1489, 2433
HincII GTYRAC 1 cut(s) 1308
HindII GTYRAC 1 cut(s) 1308
HindIII AAGCTT 3 cut(s) 1189, 2765, 3249
HpaII CCGG 2 cut(s) 1489, 2433
HphI GGTGA 7 cut(s) 92, 362, 977, 1058, 1514, 2222, 3146
Hpy99I CGWCG 2 cut(s) 94, 822
HpyAV CCTTC 8 cut(s) 364, 667, 1169, 1620, 2254, 2491, 2767, 3345
HpyCH4IV ACGT 2 cut(s) 783, 1080
HpyF10VI GCNNNNNNNGC 5 cut(s) 95, 812, 1397, 3199, 3208
HpySE526I ACGT 2 cut(s) 783, 1080
KpnI GGTACC 1 cut(s) 2197
Kzo9I GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
LmnI GCTCC 5 cut(s) 442, 1023, 1264, 1547, 2520
Lsp1109I GCAGC 4 cut(s) 65, 192, 208, 2295
LweI GCATC 5 cut(s) 247, 1156, 1387, 2019, 2931
MabI ACCWGGT 1 cut(s) 2460
MaeII ACGT 2 cut(s) 783, 1080
MaeIII GTNAC 9 cut(s) 103, 130, 934, 1129, 1537, 1773, 2081, 3038, 3109
MalI GATC 8 cut(s) 1065, 1482, 1712, 1824, 2298, 2320, 2581, 2907
MboI GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
MfeI CAATTG 3 cut(s) 126, 1907, 2285
MflI RGATCY 4 cut(s) 1063, 2296, 2318, 2579
MhlI GDGCHC 4 cut(s) 150, 462, 817, 1595
MlsI TGGCCA 1 cut(s) 1567
MluNI TGGCCA 1 cut(s) 1567
MlyI GAGTC 4 cut(s) 397, 1307, 1688, 3318
MmeI TCCRAC 8 cut(s) 145, 1001, 1680, 1728, 1815, 1834, 1929, 2325
Mox20I TGGCCA 1 cut(s) 1567
Mph1103I ATGCAT 2 cut(s) 1402, 1479
MroXI GAANNNNTTC 4 cut(s) 1932, 1980, 2171, 3222
MscI TGGCCA 1 cut(s) 1567
MslI CAYNNNNRTG 1 cut(s) 2409
Msp20I TGGCCA 1 cut(s) 1567
MspA1I CMGCKG 2 cut(s) 418, 2675
MspCI CTTAAG 2 cut(s) 1424, 2515
MspI CCGG 2 cut(s) 1489, 2433
MspR9I CCNGG 7 cut(s) 1490, 1627, 2294, 2434, 2462, 2681, 3318
MunI CAATTG 3 cut(s) 126, 1907, 2285
Mva1269I GAATGC 2 cut(s) 1479, 1655
MvaI CCWGG 5 cut(s) 1627, 2294, 2462, 2681, 3318
MwoI GCNNNNNNNGC 5 cut(s) 95, 812, 1397, 3199, 3208
NciI CCSGG 2 cut(s) 1490, 2434
NdeI CATATG 3 cut(s) 64, 1393, 1543
NdeII GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
NlaIV GGNNCC 6 cut(s) 30, 149, 1180, 1793, 2195, 2437
NmuCI GTSAC 4 cut(s) 1129, 1537, 1773, 3038
NsiI ATGCAT 2 cut(s) 1402, 1479
NspI RCATGY 3 cut(s) 2414, 2659, 3193
NspV TTCGAA 1 cut(s) 1807
PagI TCATGA 2 cut(s) 2487, 2794
PaqCI CACCTGC 1 cut(s) 436
PciI ACATGT 1 cut(s) 2655
PcsI WCGNNNNNNNCGW 1 cut(s) 817
PctI GAATGC 2 cut(s) 1479, 1655
PdmI GAANNNNTTC 4 cut(s) 1932, 1980, 2171, 3222
PfeI GAWTC 7 cut(s) 154, 551, 1430, 1933, 1981, 2158, 2553
Pfl23II CGTACG 1 cut(s) 974
PflMI CCANNNNNTGG 1 cut(s) 2069
PfoI TCCNGGA 1 cut(s) 1488
PkrI GCNGC 4 cut(s) 55, 182, 223, 2310
PleI GAGTC 4 cut(s) 397, 1307, 1688, 3317
PpsI GAGTC 4 cut(s) 397, 1307, 1688, 3317
PscI ACATGT 1 cut(s) 2655
PshBI ATTAAT 2 cut(s) 1203, 2928
PsiI TTATAA 1 cut(s) 1245
Psp124BI GAGCTC 1 cut(s) 462
Psp6I CCWGG 5 cut(s) 1625, 2292, 2460, 2679, 3316
PspGI CCWGG 5 cut(s) 1625, 2292, 2460, 2679, 3316
PspLI CGTACG 1 cut(s) 974
PspN4I GGNNCC 6 cut(s) 30, 149, 1180, 1793, 2195, 2437
PspPI GGNCC 3 cut(s) 28, 2436, 3287
PstI CTGCAG 2 cut(s) 381, 2752
PstNI CAGNNNCTG 2 cut(s) 1276, 1316
PsuI RGATCY 4 cut(s) 1063, 2296, 2318, 2579
PvuII CAGCTG 1 cut(s) 2675
RsaI GTAC 9 cut(s) 623, 714, 976, 1159, 1684, 1737, 2108, 2195, 3078
RsaNI GTAC 9 cut(s) 622, 713, 975, 1158, 1683, 1736, 2107, 2194, 3077
RseI CAYNNNNRTG 1 cut(s) 2409
SacI GAGCTC 1 cut(s) 462
SalI GTCGAC 1 cut(s) 1306
SatI GCNGC 4 cut(s) 54, 181, 222, 2309
Sau3AI GATC 8 cut(s) 1063, 1480, 1710, 1822, 2296, 2318, 2579, 2905
Sau96I GGNCC 3 cut(s) 28, 2436, 3287
ScaI AGTACT 3 cut(s) 1159, 1737, 2108
SchI GAGTC 4 cut(s) 397, 1307, 1688, 3318
ScrFI CCNGG 7 cut(s) 1490, 1627, 2294, 2434, 2462, 2681, 3318
SduI GDGCHC 4 cut(s) 150, 462, 817, 1595
SexAI ACCWGGT 1 cut(s) 2460
SfaNI GCATC 5 cut(s) 247, 1156, 1387, 2019, 2931
SfcI CTRYAG 3 cut(s) 377, 1778, 2748
SfuI TTCGAA 1 cut(s) 1807
SinI GGWCC 1 cut(s) 2436
SmiMI CAYNNNNRTG 1 cut(s) 2409
SmlI CTYRAG 5 cut(s) 1183, 1424, 2023, 2261, 2515
SmoI CTYRAG 5 cut(s) 1183, 1424, 2023, 2261, 2515
SsiI CCGC 3 cut(s) 416, 1107, 1925
SspI AATATT 1 cut(s) 2807
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 7 cut(s) 1488, 1625, 2292, 2432, 2460, 2679, 3316
StyI CCWWGG 1 cut(s) 1643
TaiI ACGT 2 cut(s) 786, 1083
TaqI TCGA 9 cut(s) 279, 324, 1307, 1807, 2392, 2760, 2778, 3051, 3123
TaqII GACCGA 1 cut(s) 1761
TatI WGTACW 3 cut(s) 1157, 1735, 2106
TfiI GAWTC 7 cut(s) 154, 551, 1430, 1933, 1981, 2158, 2553
TscAI CASTG 9 cut(s) 858, 1386, 1416, 1470, 1600, 1644, 2070, 2428, 2757
TseFI GTSAC 4 cut(s) 1129, 1537, 1773, 3038
TseI GCWGC 4 cut(s) 53, 180, 221, 2308
Tsp45I GTSAC 4 cut(s) 1129, 1537, 1773, 3038
TspGWI ACGGA 2 cut(s) 1973, 3266
TspRI CASTG 9 cut(s) 858, 1386, 1416, 1470, 1600, 1644, 2070, 2428, 2757
Van91I CCANNNNNTGG 1 cut(s) 2069
Vha464I CTTAAG 2 cut(s) 1424, 2515
VneI GTGCAC 1 cut(s) 1591
VpaK11BI GGWCC 1 cut(s) 2436
VspI ATTAAT 2 cut(s) 1203, 2928
XapI RAATTY 7 cut(s) 747, 759, 986, 1037, 1891, 2700, 3008
XbaI TCTAGA 3 cut(s) 941, 2299, 2704
XceI RCATGY 3 cut(s) 2414, 2659, 3193
XcmI CCANNNNNNNNNTGG 3 cut(s) 613, 1641, 2204
XmiI GTMKAC 3 cut(s) 333, 1221, 1307
XmnI GAANNNNTTC 4 cut(s) 1932, 1980, 2171, 3222
ZrmI AGTACT 3 cut(s) 1159, 1737, 2108
Zsp2I ATGCAT 2 cut(s) 1402, 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.