Rroxscaffold_3G00253850

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000042
Physical Location & Seq
Reverse (-)
48059341 .. 48072292
12952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00253850.1

Sequence Viewer

Length: 2949 bp
ATGCGTACACAGAAAGATATTTCAGTTGATGTTTTAAATGAAGAGGAAGCATGGAGATTATTTGAGAAGATGGCTGGTGATGTTGTCAGAGACAGCCGGATAAAAAGCGTAGCATTTGAAGTAGCCAAAAAATGTGGAGGTTTGCCGGTCTTGATTGTTACGGTTGCAAGAGCTCTAAAAGACAGAAACAAGTTGCAAATATGGAAAGACACCTTGAGACGCCTTAGAGGCTTTGACAACAAAGAATTTACGGAAAAAGCATACTCGGCTCTGGAGTGGAGTTATGATCAATTGGAAGATAAAGAGCTTAAGCCGTTGTTCTTGCTTTGTGGGATTATTGTAGTGAAGAACCATATTAATTTGGCAGACTTGTTGAAATATGGTTTTGGTTTGGGTTTGATTAAAAATGTCGATACAATAGAAGAAGCACGAGATGCATTGGATGCACAAATTGAAAAGCTTAAAGATTCTTGCTTGTTGCTGGACGGTGATATCAATATGCATGCTAGAATGCATGATCTTGTATGTGAGGTTGCTACACGGATTGCATCCAGAGATCAACATGTCTTATCAGTTGGATATGGAGGTGAACTACAAGAAAGGCCTGATAAAGATTTCTTTGAAAGGTGCAGTAAGATGTCTTTAAAATCCAGCAATATTCCCATGCTTTCTGAAGTACCATGGAAATGCCCGAAACTAGAGATGTTTCATTTGTACAATAAAGATGGTGCATCATCGATGGAAATCCCAGCCAACTTTTTTAGTGAAATGGAGAAACTGAAAGTATTGGGTTTAACTGAAGTGCGTATGCGGTCACTACCCCCATCTCTTCAGCTCTTGAAGAATCTTCAGACCTTGTGTTTAGAAGAATGTTGGTTGGAAGATGTAGCTCTAGTTGGATATTTAAGCAACTTGGAGATTCTTAGCTTTGCGCGGTCCTACATTGAACAGCTACCCGTAGAAATAGGGCAATTGATTCGATTGAGATGGTTGGATTTGAGTGATTGTGTCGGACTTCGAGTGATTTCACCTGGTGTTGTTTCAAGGTTGGAAAGACTAGAAGAGTTATCACTCAGAAACGTCTTTCTCAAATGGGAGGCTGAAAGGGGAGGAAGTAATGCTAGTCTTTTAGAGCTAAAAGACTTGTCTCAACTAACTGCATTGGAGATATATATTGAAGATGCAAATATTCTTCCATCAAACTTGTTCTCCAGTAAGTTGGTAAGATACCAAATATATATTGGCGATGAATGGGAGCCGCCTACGGCTGAAACGACCCTCAACACGCTAAAACTGAAGCTCGCTCCCAGCGATGAATTGGACCGCGGTATCAAAACATTGTTGAAGAGATCTGAAGATTTGTCTTTGGATGGAACAGAGAGTGTTAATAGTATTGTCCGTCAATTAGATGTTGAAGATTTTCAGCAATTGATACACCTCCGTCTCGAAAACAATGTTGATTTCACCTATATCATTAATAGGAAGTTTGTGTTCCCCAACTTAACAACCTTGAGCATATGTAGCTGTGACCATCTAAGTTTCTTGTTCTCATTTTCTATGGCTAAAAGTTTGGTGGAGCTCAAACATCTTGAAGTATCCAGATGTGAAATAATGGAAGAGATAGTATCAGCAAGAGATCAATCCAATGGAGAAAATATCGATATCATGTTTTGTAAGTTGGAAAGTCTAATACTAGATGAACTCCCAAACATCACTAGATTCTGTACTGAAAGTTGTACTGAATTTTCTCCTGTGACGAAATTTGATATGGAACTGGGGCCATCCATCTTTGATCCTACGGGTAGTAAAAAACTTGATGTCAAGAATGATACTGCTGTACGAGAATGTCTTTTTGACACACAGGTAAAATTTCCGAGCTTGGAGAGACTGAAAATCGATGGGCTAGGGAAGTTGCACACGCTATGGAACAATAAATTTGCTGATCAAGACTCTTTTTGCCAACTCAAAGAAGTTGAAGTTTCAAATTGCAACAGTCTGAAAAAGATCTTTCCAGTCTCAGTAGCCAGACGTCTCCAAAAGCTACATTCGCTGGATGTGGAAGACTGTGATGAAGTGGAGGAAATCATTGCTGGTGAACAAGGACTAGAAACAGAGTCTCCTCATTTAGTGTTCCCAAAAGTAACACGTGTGACATTTTATGGTCTGCCCAAACTAAGTAGATTCTATCCTGGGATTATTGCTTCCAAGTGGCCGTTACTCAAACAACTAGACATTTTTGAATGTGGGAAAGTGAAGATTATTGCCGCAGAACTTCCAATTTCTTCAGAAAGACATGAGCCGAACCCTCTAATTGAACAATCTCTCTTTCTAATTGACAAGGATTCATTTCCCAACTTGGAAGAGTTAAGATTGGAAGTTGCGGGTGAGATTTGGTACGGTCCATCATCCCCAGCACACTTCTTTCCCAAATTAAAAACTATTCGCTTTTACGAGTGGGCCGCGTTCTCCAAGCAAGCCGCAATACATTTCCTTGAGAAATTACCGAATCTTGAGGAGATAGAAGTGGGCTGGGATGATGTTTCCACGGAAATATTTGTGAATGAAGAAATTAATGGCGGTGAGGAGATACATGCACTTGGGACGACCCTCTCAGGTGTCAAAACCTTGACGGTCCATGACATGCCCAAGCTCTTGCAATTAGGGAATGGGAACTCTCAAACAGCGGGCCCACTTTTCCCAAACTTGGACACTCTTAGGGTGTATCGGTGTGAGATGTTAAAGAGTGTAGAGTCATCCGCAGCAATATCCTTCCGCAATCTAACAACTCTGCAAGTGTGGGGTTGTCATGGATTGGAATACTTGACAAGTTATTCCGTAGCTAAGAGCTTAATGCAACTGACAACACTGGATGTGAGTGGTTGTAAAAGCCTGAGAGCAATTATAGGGGCAAGCAACGAAGATGAAGGAGTTTTAAGATCCCCAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

982

Amino Acids

111.32

Weight (kDa)

5.11

Isoelectric Point (pI)

49.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 248 - 532 8.7e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 488 - 577 2.7e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 621 - 690 1.2e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 794 - 916 1e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 921 - 962 2.8e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2032
AccII CGCG 3 cut(s) 934, 1326, 2462
AclWI GGATC 2 cut(s) 1787, 2931
AcoI YGGCCR 1 cut(s) 2210
AcsI RAATTY 5 cut(s) 245, 1742, 1760, 1868, 1934
AcuI CTGAAG 7 cut(s) 693, 815, 819, 833, 1316, 1374, 2268
AcvI CACGTG 1 cut(s) 2147
AcyI GRCGYC 2 cut(s) 220, 2029
AdeI CACNNNGTG 1 cut(s) 1034
AfaI GTAC 7 cut(s) 7, 678, 716, 1726, 1738, 1839, 2396
AfiI CCNNNNNNNGG 1 cut(s) 2704
AflII CTTAAG 1 cut(s) 308
AflIII ACRYGT 3 cut(s) 562, 2144, 2146
AjnI CCWGG 3 cut(s) 1030, 2188, 2941
Alw21I GWGCWC 2 cut(s) 175, 1581
Alw26I GTCTC 8 cut(s) 84, 211, 1152, 1448, 1879, 2020, 2036, 2121
AlwI GGATC 2 cut(s) 1787, 2931
AoxI GGCC 5 cut(s) 602, 1778, 2210, 2457, 2686
ApaI GGGCCC 1 cut(s) 2690
ApeKI GCWGC 1 cut(s) 2759
ApoI RAATTY 5 cut(s) 245, 1742, 1760, 1868, 1934
AseI ATTAAT 3 cut(s) 357, 1476, 2571
Asp700I GAANNNNTTC 1 cut(s) 1419
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 8 cut(s) 936, 1321, 1778, 2399, 2457, 2632, 2686, 2687
AsuHPI GGTGA 8 cut(s) 89, 500, 599, 1020, 1456, 2105, 2396, 2591
AvaII GGWCC 4 cut(s) 936, 1321, 2399, 2632
BaeGI GKGCMC 1 cut(s) 2690
BaeI ACNNNNGTAYC 4 cut(s) 1312, 1345, 1821, 1854
BanII GRGCYC 3 cut(s) 175, 1581, 2690
BauI CACGAG 1 cut(s) 429
BbrPI CACGTG 1 cut(s) 2147
BbsI GAAGAC 1 cut(s) 2067
Bbv12I GWGCWC 2 cut(s) 175, 1581
BbvI GCAGC 1 cut(s) 2771
BceAI ACGGC 3 cut(s) 298, 1281, 2197
BcgI CGANNNNNNTGC 2 cut(s) 959, 993
BciT130I CCWGG 3 cut(s) 1032, 2190, 2943
BciVI GTATCC 1 cut(s) 1606
BclI TGATCA 2 cut(s) 286, 1942
BcoDI GTCTC 8 cut(s) 84, 211, 1152, 1448, 1879, 2020, 2036, 2121
BfrI CTTAAG 1 cut(s) 308
BfuI GTATCC 1 cut(s) 1606
BglI GCCNNNNNGGC 1 cut(s) 228
BglII AGATCT 2 cut(s) 1349, 2004
BisI GCNGC 5 cut(s) 1259, 2265, 2460, 2478, 2760
BlsI GCNGC 5 cut(s) 1260, 2266, 2461, 2479, 2761
Bme1390I CCNGG 3 cut(s) 1032, 2190, 2943
Bme18I GGWCC 4 cut(s) 936, 1321, 2399, 2632
BmgT120I GGNCC 8 cut(s) 936, 1321, 1778, 2399, 2457, 2632, 2686, 2687
BmiI GGNNCC 3 cut(s) 1257, 1779, 2688
BmrFI CCNGG 3 cut(s) 1032, 2190, 2943
BmrI ACTGGG 1 cut(s) 1784
BmsI GCATC 5 cut(s) 424, 433, 557, 740, 1171
BmuI ACTGGG 1 cut(s) 1784
BpiI GAAGAC 1 cut(s) 2067
BpmI CTGGAG 2 cut(s) 293, 1195
BpuEI CTTGAG 4 cut(s) 235, 1531, 2513, 2531
Bsa29I ATCGAT 3 cut(s) 737, 1659, 1896
BsaAI YACGTR 1 cut(s) 2147
BsaBI GATNNNNATC 1 cut(s) 743
BsaHI GRCGYC 2 cut(s) 220, 2029
BsaJI CCNNGG 5 cut(s) 680, 1324, 2189, 2544, 2941
BsaXI ACNNNNNCTCC 4 cut(s) 1193, 1223, 2101, 2131
Bsc4I CCNNNNNNNGG 1 cut(s) 2704
Bse118I RCCGGY 1 cut(s) 145
Bse1I ACTGG 4 cut(s) 1212, 1779, 2012, 2871
Bse3DI GCAATG 1 cut(s) 2085
Bse8I GATNNNNATC 1 cut(s) 743
BseBI CCWGG 3 cut(s) 1032, 2190, 2943
BseCI ATCGAT 3 cut(s) 737, 1659, 1896
BseDI CCNNGG 5 cut(s) 680, 1324, 2189, 2544, 2941
BseGI GGATG 9 cut(s) 448, 548, 1375, 1781, 2059, 2405, 2539, 2753, 2875
BseJI GATNNNNATC 1 cut(s) 743
BseLI CCNNNNNNNGG 1 cut(s) 2704
BseMI GCAATG 1 cut(s) 2085
BseMII CTCAG 4 cut(s) 1087, 2031, 2625, 2882
BseNI ACTGG 4 cut(s) 1212, 1779, 2012, 2871
BseRI GAGGAG 3 cut(s) 2109, 2528, 2597
BseSI GKGCMC 1 cut(s) 2690
BseXI GCAGC 1 cut(s) 2771
BseYI CCCAGC 4 cut(s) 748, 1307, 2410, 2529
BsgI GTGCAG 1 cut(s) 649
Bsh1236I CGCG 3 cut(s) 934, 1326, 2462
BshFI GGCC 5 cut(s) 604, 1780, 2212, 2459, 2688
BshVI ATCGAT 3 cut(s) 737, 1659, 1896
BsiHKAI GWGCWC 2 cut(s) 175, 1581
BsiSI CCGG 2 cut(s) 97, 146
BslFI GGGAC 1 cut(s) 2614
BslI CCNNNNNNNGG 1 cut(s) 2704
BsmAI GTCTC 8 cut(s) 84, 211, 1152, 1448, 1879, 2020, 2036, 2121
BsmBI CGTCTC 3 cut(s) 211, 1448, 2036
BsmFI GGGAC 1 cut(s) 2614
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 5 cut(s) 604, 1780, 2212, 2459, 2688
Bsp120I GGGCCC 1 cut(s) 2686
Bsp1286I GDGCHC 3 cut(s) 175, 1581, 2690
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
Bsp19I CCATGG 1 cut(s) 680
BspANI GGCC 5 cut(s) 604, 1780, 2212, 2459, 2688
BspCNI CTCAG 4 cut(s) 1086, 2030, 2624, 2883
BspDI ATCGAT 3 cut(s) 737, 1659, 1896
BspFNI CGCG 3 cut(s) 934, 1326, 2462
BspLI GGNNCC 3 cut(s) 1257, 1779, 2688
BspPI GGATC 2 cut(s) 1787, 2931
BspTI CTTAAG 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 2085
BsrFI RCCGGY 1 cut(s) 145
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 4 cut(s) 1212, 1779, 2012, 2871
BssAI RCCGGY 1 cut(s) 145
BssECI CCNNGG 5 cut(s) 680, 1324, 2189, 2544, 2941
BssMI GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
BssNI GRCGYC 2 cut(s) 220, 2029
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 3 cut(s) 1032, 2190, 2943
Bst4CI ACNGT 6 cut(s) 163, 488, 1994, 2066, 2399, 2632
Bst6I CTCTTC 6 cut(s) 36, 834, 1056, 1340, 1611, 2355
BstACI GRCGYC 2 cut(s) 220, 2029
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 2 cut(s) 434, 443
BstAUI TGTACA 1 cut(s) 714
BstBAI YACGTR 1 cut(s) 2147
BstC8I GCNNGC 5 cut(s) 504, 1302, 2475, 2686, 2911
BstDSI CCRYGG 3 cut(s) 680, 1324, 2544
BstF5I GGATG 9 cut(s) 448, 548, 1375, 1781, 2059, 2405, 2539, 2753, 2875
BstFNI CGCG 3 cut(s) 934, 1326, 2462
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 9 cut(s) 289, 520, 559, 1352, 1639, 1795, 1945, 2007, 2939
BstMAI GTCTC 8 cut(s) 84, 211, 1152, 1448, 1879, 2020, 2036, 2121
BstMBI GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
BstMWI GCNNNNNNNGC 6 cut(s) 228, 266, 434, 443, 1521, 2047
BstNI CCWGG 3 cut(s) 1032, 2190, 2943
BstNSI RCATGY 4 cut(s) 506, 566, 2594, 2644
BstSCI CCNGG 3 cut(s) 1030, 2188, 2941
BstSLI GKGCMC 1 cut(s) 2690
BstUI CGCG 3 cut(s) 934, 1326, 2462
BstV1I GCAGC 1 cut(s) 2771
BstV2I GAAGAC 1 cut(s) 2067
BstX2I RGATCY 3 cut(s) 1349, 2004, 2936
BstXI CCANNNNNNTGG 1 cut(s) 1219
BstYI RGATCY 3 cut(s) 1349, 2004, 2936
Bsu15I ATCGAT 3 cut(s) 737, 1659, 1896
BsuI GTATCC 1 cut(s) 1606
BsuRI GGCC 5 cut(s) 604, 1780, 2212, 2459, 2688
BsuTUI ATCGAT 3 cut(s) 737, 1659, 1896
BtgI CCRYGG 3 cut(s) 680, 1324, 2544
BtgZI GCGATG 2 cut(s) 1260, 1326
BtsCI GGATG 9 cut(s) 448, 548, 1375, 1781, 2059, 2405, 2539, 2753, 2875
BtsIMutI CAGTG 1 cut(s) 2864
Cac8I GCNNGC 5 cut(s) 504, 1302, 2475, 2686, 2911
CfoI GCGC 1 cut(s) 934
Cfr10I RCCGGY 1 cut(s) 145
Cfr13I GGNCC 8 cut(s) 936, 1321, 1778, 2399, 2457, 2632, 2686, 2687
Cfr42I CCGCGG 1 cut(s) 1327
ClaI ATCGAT 3 cut(s) 737, 1659, 1896
CseI GACGC 1 cut(s) 228
CsiI ACCWGGT 1 cut(s) 1030
Csp6I GTAC 7 cut(s) 6, 677, 715, 1725, 1737, 1838, 2395
CspCI CAANNNNNGTGG 2 cut(s) 115, 150
CviQI GTAC 7 cut(s) 6, 677, 715, 1725, 1737, 1838, 2395
DpnI GATC 9 cut(s) 288, 519, 558, 1351, 1638, 1794, 1944, 2006, 2938
DpnII GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
DraI TTTAAA 2 cut(s) 36, 645
DraIII CACNNNGTG 1 cut(s) 1034
EaeI YGGCCR 1 cut(s) 2210
Eam1104I CTCTTC 6 cut(s) 36, 834, 1056, 1340, 1611, 2355
EarI CTCTTC 6 cut(s) 36, 834, 1056, 1340, 1611, 2355
Ecl136II GAGCTC 2 cut(s) 173, 1579
Eco130I CCWWGG 1 cut(s) 680
Eco147I AGGCCT 1 cut(s) 604
Eco24I GRGCYC 3 cut(s) 175, 1581, 2690
Eco32I GATATC 2 cut(s) 493, 1663
Eco47I GGWCC 4 cut(s) 936, 1321, 2399, 2632
Eco53kI GAGCTC 2 cut(s) 173, 1579
Eco57I CTGAAG 7 cut(s) 693, 815, 819, 833, 1316, 1374, 2268
Eco72I CACGTG 1 cut(s) 2147
EcoICRI GAGCTC 2 cut(s) 173, 1579
EcoRII CCWGG 3 cut(s) 1030, 2188, 2941
EcoRV GATATC 2 cut(s) 493, 1663
EcoT14I CCWWGG 1 cut(s) 680
EcoT22I ATGCAT 3 cut(s) 439, 504, 516
EcoT38I GRGCYC 3 cut(s) 175, 1581, 2690
ErhI CCWWGG 1 cut(s) 680
Esp3I CGTCTC 3 cut(s) 211, 1448, 2036
FaqI GGGAC 1 cut(s) 2614
FauI CCCGC 2 cut(s) 2374, 2677
FauNDI CATATG 1 cut(s) 1517
FbaI TGATCA 2 cut(s) 286, 1942
Fnu4HI GCNGC 5 cut(s) 1259, 2265, 2460, 2478, 2760
FokI GGATG 9 cut(s) 455, 535, 1382, 1768, 2066, 2392, 2546, 2740, 2882
FriOI GRGCYC 3 cut(s) 175, 1581, 2690
Fsp4HI GCNGC 5 cut(s) 1259, 2265, 2460, 2478, 2760
GlaI GCGC 1 cut(s) 933
GluI GCNGC 5 cut(s) 1259, 2265, 2460, 2478, 2760
GsaI CCCAGC 4 cut(s) 752, 1311, 2414, 2533
GsuI CTGGAG 2 cut(s) 293, 1195
HaeIII GGCC 5 cut(s) 604, 1780, 2212, 2459, 2688
HapII CCGG 2 cut(s) 97, 146
HgaI GACGC 1 cut(s) 228
HhaI GCGC 1 cut(s) 934
Hin1I GRCGYC 2 cut(s) 220, 2029
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HindIII AAGCTT 1 cut(s) 458
HpaII CCGG 2 cut(s) 97, 146
HphI GGTGA 8 cut(s) 89, 500, 599, 1020, 1456, 2105, 2396, 2591
Hpy166II GTNNAC 3 cut(s) 8, 590, 2096
Hpy188I TCNGA 9 cut(s) 89, 673, 852, 1013, 1076, 1354, 1875, 1998, 2287
Hpy8I GTNNAC 3 cut(s) 8, 590, 2096
HpyAV CCTTC 2 cut(s) 2779, 2918
HpyCH4III ACNGT 6 cut(s) 163, 488, 1994, 2066, 2399, 2632
HpyCH4IV ACGT 3 cut(s) 1080, 2029, 2146
HpyF10VI GCNNNNNNNGC 6 cut(s) 228, 266, 434, 443, 1521, 2047
HpySE526I ACGT 3 cut(s) 1080, 2029, 2146
Hsp92I GRCGYC 2 cut(s) 220, 2029
HspAI GCGC 1 cut(s) 932
Ksp22I TGATCA 2 cut(s) 286, 1942
KspI CCGCGG 1 cut(s) 1327
Kzo9I GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
LmnI GCTCC 3 cut(s) 1255, 1309, 1576
Lsp1109I GCAGC 1 cut(s) 2771
LweI GCATC 5 cut(s) 424, 433, 557, 740, 1171
MabI ACCWGGT 1 cut(s) 1030
MaeII ACGT 3 cut(s) 1080, 2029, 2146
MaeIII GTNAC 7 cut(s) 157, 813, 1526, 1753, 2140, 2149, 2214
MalI GATC 9 cut(s) 288, 519, 558, 1351, 1638, 1794, 1944, 2006, 2938
MboI GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
MfeI CAATTG 3 cut(s) 290, 971, 1427
MflI RGATCY 3 cut(s) 1349, 2004, 2936
MhlI GDGCHC 3 cut(s) 175, 1581, 2690
MlyI GAGTC 3 cut(s) 1943, 2123, 2759
MmeI TCCRAC 7 cut(s) 556, 858, 877, 972, 991, 1029, 1659
Mph1103I ATGCAT 3 cut(s) 439, 504, 516
MroXI GAANNNNTTC 1 cut(s) 1419
MslI CAYNNNNRTG 1 cut(s) 685
MspA1I CMGCKG 2 cut(s) 1326, 2684
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 2 cut(s) 97, 146
MspR9I CCNGG 3 cut(s) 1032, 2190, 2943
MunI CAATTG 3 cut(s) 290, 971, 1427
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 3 cut(s) 1032, 2190, 2943
MvnI CGCG 3 cut(s) 934, 1326, 2462
MwoI GCNNNNNNNGC 6 cut(s) 228, 266, 434, 443, 1521, 2047
NcoI CCATGG 1 cut(s) 680
NdeI CATATG 1 cut(s) 1517
NdeII GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
NlaIV GGNNCC 3 cut(s) 1257, 1779, 2688
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 4 cut(s) 813, 1526, 1753, 2149
NsiI ATGCAT 3 cut(s) 439, 504, 516
NspI RCATGY 4 cut(s) 506, 566, 2594, 2644
PaeI GCATGC 1 cut(s) 506
PceI AGGCCT 1 cut(s) 604
PciI ACATGT 1 cut(s) 562
PcsI WCGNNNNNNNCGW 2 cut(s) 1271, 1308
PctI GAATGC 1 cut(s) 516
PdmI GAANNNNTTC 1 cut(s) 1419
PfeI GAWTC 8 cut(s) 467, 844, 919, 976, 1719, 2181, 2342, 2506
PkrI GCNGC 5 cut(s) 1260, 2266, 2461, 2479, 2761
PleI GAGTC 3 cut(s) 1943, 2122, 2758
PmaCI CACGTG 1 cut(s) 2147
PmlI CACGTG 1 cut(s) 2147
PpsI GAGTC 3 cut(s) 1943, 2122, 2758
Ppu21I YACGTR 1 cut(s) 2147
PscI ACATGT 1 cut(s) 562
PshBI ATTAAT 3 cut(s) 357, 1476, 2571
Psp124BI GAGCTC 2 cut(s) 175, 1581
Psp6I CCWGG 3 cut(s) 1030, 2188, 2941
PspCI CACGTG 1 cut(s) 2147
PspFI CCCAGC 4 cut(s) 748, 1307, 2410, 2529
PspGI CCWGG 3 cut(s) 1030, 2188, 2941
PspN4I GGNNCC 3 cut(s) 1257, 1779, 2688
PspOMI GGGCCC 1 cut(s) 2686
PspPI GGNCC 8 cut(s) 936, 1321, 1778, 2399, 2457, 2632, 2686, 2687
PsuI RGATCY 3 cut(s) 1349, 2004, 2936
RsaI GTAC 7 cut(s) 7, 678, 716, 1726, 1738, 1839, 2396
RsaNI GTAC 7 cut(s) 6, 677, 715, 1725, 1737, 1838, 2395
RseI CAYNNNNRTG 1 cut(s) 685
SacI GAGCTC 2 cut(s) 175, 1581
SacII CCGCGG 1 cut(s) 1327
SatI GCNGC 5 cut(s) 1259, 2265, 2460, 2478, 2760
Sau3AI GATC 9 cut(s) 286, 517, 556, 1349, 1636, 1792, 1942, 2004, 2936
Sau96I GGNCC 8 cut(s) 936, 1321, 1778, 2399, 2457, 2632, 2686, 2687
SchI GAGTC 3 cut(s) 1943, 2123, 2759
ScrFI CCNGG 3 cut(s) 1032, 2190, 2943
SduI GDGCHC 3 cut(s) 175, 1581, 2690
SexAI ACCWGGT 1 cut(s) 1030
SfaNI GCATC 5 cut(s) 424, 433, 557, 740, 1171
Sfr303I CCGCGG 1 cut(s) 1327
SgrBI CCGCGG 1 cut(s) 1327
SinI GGWCC 4 cut(s) 936, 1321, 2399, 2632
SmiMI CAYNNNNRTG 1 cut(s) 685
SmlI CTYRAG 5 cut(s) 214, 308, 1510, 2492, 2510
SmoI CTYRAG 5 cut(s) 214, 308, 1510, 2492, 2510
SphI GCATGC 1 cut(s) 506
SseBI AGGCCT 1 cut(s) 604
SspI AATATT 3 cut(s) 658, 1189, 2553
SstI GAGCTC 2 cut(s) 175, 1581
StuI AGGCCT 1 cut(s) 604
StyD4I CCNGG 3 cut(s) 1030, 2188, 2941
StyI CCWWGG 1 cut(s) 680
TaaI ACNGT 6 cut(s) 163, 488, 1994, 2066, 2399, 2632
TaiI ACGT 3 cut(s) 1083, 2032, 2149
TaqI TCGA 7 cut(s) 411, 737, 979, 1018, 1446, 1659, 1896
TatI WGTACW 3 cut(s) 714, 1724, 1736
TauI GCSGC 4 cut(s) 1261, 2267, 2462, 2480
TfiI GAWTC 8 cut(s) 467, 844, 919, 976, 1719, 2181, 2342, 2506
TscAI CASTG 1 cut(s) 2871
TseFI GTSAC 4 cut(s) 813, 1526, 1753, 2149
TseI GCWGC 1 cut(s) 2759
Tsp45I GTSAC 4 cut(s) 813, 1526, 1753, 2149
TspDTI ATGAA 9 cut(s) 54, 698, 1263, 1329, 1713, 2085, 2334, 2577, 2937
TspGWI ACGGA 6 cut(s) 266, 556, 1388, 1430, 2561, 2824
TspRI CASTG 1 cut(s) 2871
Vha464I CTTAAG 1 cut(s) 308
VpaK11BI GGWCC 4 cut(s) 936, 1321, 2399, 2632
VspI ATTAAT 3 cut(s) 357, 1476, 2571
XapI RAATTY 5 cut(s) 245, 1742, 1760, 1868, 1934
XceI RCATGY 4 cut(s) 506, 566, 2594, 2644
XcmI CCANNNNNNNNNTGG 2 cut(s) 1238, 1315
XmnI GAANNNNTTC 1 cut(s) 1419
ZraI GACGTC 1 cut(s) 2030
Zsp2I ATGCAT 3 cut(s) 439, 504, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.