Rroxscaffold_1G00060530

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000042
Physical Location & Seq
Reverse (-)
82726472 .. 82733995
7524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00060530.1

Sequence Viewer

Length: 3054 bp
ATGGAAATTTCTGTAAATGAAAGGGAAACTGTTGATGGAAGAGCGAGTCGTTTATGTGCCAGGATACAAGGCAAAAAGATTCTTGTGATATTGGATGATGTTCAAGAAAAAATTGATTTGGAGGTTGTGGGACTTCCCCGTTTGCCCACGTGTAAAATTTTGGTGACATGTAGAACTGCAGATGTTTTATCTTTTGGTAAGATGTCTGCAGATAAAGTGTTTTCGCTTGACCTTCTAGGTAAAGATGAAACTTGGAATTTGTTTGTGAAGATGGCCGGTGACATTGTTAAACCCAATAGTCAAATACGAGATGTAGCGATCCAAGTAGCCGAAAAATGTGGAGGTCTGCCCTTATTAGTTGTCACAGTTGCAAGTGCTTTGAAAGGAAAAAAGAAATTAGGTGAATGGAAAGACGCCTTAAGACGACTTAAAGGGTTTGATAAAGAAGAATTGACGGAAAAGGCATACCTAGCTTTAGAGTGGAGTTATAACCAATTAGATGATAAGGAGCTTAAACCGTTGTTCTTGCTATCCGGAATCATAGTGCAGCAAAACCATATTTATGTGCAAGACTTGTTGAAATATGGTGTGGGTTTAGGTTTGTTTACAAATGTGAATACATTGGAAGAAGCACGGAATGCATTGGATACACTGCTTGGAAAGCTTAAAAATTCTTGCTTGTTGCTGGACAGTGATACTGAGGGAGTTGTCAGAATGCATGATCTTGTATGTCATGTTGCTAAGCTAATTGCATACAGGGATCATCAGATGCTATCAATAGAATATGGAGGTGAGTTTCAAAAATGGCCAAATCAGGATTTCTTAAAGAAGTGCACTAGGATCTCTCTCATACCCGAAAATATTCCCACACTTCCTGAAATACCTATGGAATGCCCAGAACTAGAGCTGTTCTATTTCCACAGCATTGAATATGATGACTCGGTCCAAATCCCATCCAACTTTTTCAGTGAGATGCAGAAACTCAAAGTGCTGAATTTAACCAACATGTGTATACCATCATTGCCTTTATCTCTTCTGTACTTGAAGGAGCTTCAAACGTTGTGTTTGATTGACTGCTTGTTGGGAGATATAACTCTACTGGGGCAGCTAAGTAGTTTGGAAGTTCTTAGCTTTTCACAATCCATTGTTGGAAAATTGCCTAAAGAAATAGGGCAATTGACTCTTCTTCGGTTGTTGGATTTATCTGATTGCGATGGTCTTGAGGTGATTTCACCTACTGTTATATCTAGCTTGGTGAAACTGGAGGATTTGAATATGAGAAACAGCTTTAAGAAATGGGAAGTTGAAGGTGAAGGGAGTAATGCAAGCCTTTCAGAGCTGAAACATTTGTCTCAGCTATCCTCATTAGAAATACATATTCCGAATGTTGACGCTCTTCCAGCAAACTTGCTCTCCAACAAGTTAGAAAGGTTCAAGATTCATATTGGGGATGCATGGGATACATGGGATGCGAGATCTCCATGGAAGAAATTTAGGAATACGATGACCCTCAACACATTAAAACTCAAGCTCACTAAAGAATTAGATGAAAGCCTAAAATGGCTACTCAAGAATTCTGAAGACTTGTCCTTGGATGAGACGGAGGTTGTTATTGTCCATGATTTGAATGCCGAAGATTTTGAAAATAAGATACATCTCCAACTCCAAAACAATGTTGATTTTATGGCTATGGTCAACAAGAAGGTTGTGTTCCCAAACTTAGCAACCTTGTCAGTGCATGGATGTGATAATTTAAAAATTTTGTTCTCATCTTCCATGGCTAAAAGTCTTGTACAACTCACACATCTAAAAATATCCGCCTGTAAACTCCTGAAAGAGATTGTATCATCAACAAGAGAAGGCGGTGAAGACAATACGGATGACATGTTTTATAAACTTAAAGTTTTGAAGCTTGAAGAACTTCCAAACCTCGCTAGATTCAGCACAGGAAATTATATTGAGTTTCTGTCATTGGAAGAACTAAAGGTAAAGGGTTGTGATATTTTGGGGGTAATCATTGGTGATAAAGTATCTATGAGAAGTAAAGACACTAGAAGCTGCAAAGAAATTGAAGAGAGGATCTTGGAAGAGGAAGAGAACCTTGAAGTCAAGAGTGAGACCGTTGTGAAATACTCCCTCTTTAACGAAAAGGTCGTGTTGCGCAACTTAACAATCTTGTTGGTGCATGGATGTGATAATTTGAAATTTTTGTCCTCATTTTCCATGGCTAAAAGTCTTGAACAACTCACACATCTAAAGATATCCGCATGTAAACTTCTGCAAGATATTGTATCATCAACAAGAGAAGGCAGTGAAGACAATATGGATGGCATGTATTTTAAACTTAAAGTTTTGAAGCTTGAAGAACTTCCAAACCTCACTAGATTCAGCACAGGAAATTATATTGAGTTTCCGTCATTGGAAGAGCTGGAGGTAAAGGGTTGTGATAATCTGAGGGTATTCATTAGTGATAAAGTTTCAGTGAGTAGTAAAGACACTAGAAGCTGCAAAGAAATTGAACAAAGGGTCTTGGAAGAGGAAGAAAACCTTGAAGTCAAGAGTGAGACCGTTGTGAAATACTCCTTCTTTAACGAAAAGGTAGGATTTCCTATCTTGGAGAGATTGAGCATCGAAGGGGTAAGGGAGTTGAAGGCGATATGGCACGTCCAACAATCTCAAAACTCTTTTTGGAGACTCAAGCAAGTTGAGGTTCGGGAATGTGACAATCTAATAAGTTTGTTTCCGCCTAGTGTCATCAAAAGATTGAATGCACTGGAAACCTTGAAGATATGGAAGTGCAAATCACTAGAAGTGGTATGTGAGGAAATTGTTGTTACAGAAAAGGAACAAGTAGTTGTAGAAACAACACCTCCTCTATTTGTATTTCCAAAAGTAACATCCGTGGAGTTGGCGAATCTACCTCAACCTACGGGTTGCATAAATGCTTCACAGTTGCCATCACTCAAACGATTGGAGGTGGGGGATGTGATAAAGTGGGAATTTTTGCCACAGAATTTTCTCACTTTGGAAAGAAATATGAGATGGAACCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1017

Amino Acids

115.6

Weight (kDa)

5.36

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 9 - 93 1.2e-11 NB-ARC domain
LRR_14 PF23598 320 - 586 6.2e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 564 - 671 3.4e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 568 - 609 6.1e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 708 - 823 1.5e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 864 - 934 1.8e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 881 - 974 2e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 489, 1893
Acc16I TGCGCA 1 cut(s) 2162
AccI GTMKAC 1 cut(s) 1012
AccIII TCCGGA 1 cut(s) 533
AciI CCGC 4 cut(s) 1818, 1863, 2265, 2744
AclI AACGTT 1 cut(s) 1058
AclWI GGATC 4 cut(s) 313, 768, 848, 2087
AcoI YGGCCR 2 cut(s) 273, 806
AcuI CTGAAG 1 cut(s) 1599
AcvI CACGTG 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 2 cut(s) 1040, 1794
AflII CTTAAG 1 cut(s) 418
AflIII ACRYGT 4 cut(s) 149, 167, 1005, 1884
AjiI CACGTC 1 cut(s) 2665
AjnI CCWGG 1 cut(s) 59
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 5 cut(s) 1356, 1592, 2111, 2558, 2686
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 4 cut(s) 313, 768, 848, 2087
Aor13HI TCCGGA 1 cut(s) 533
AoxI GGCC 2 cut(s) 273, 806
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 4 cut(s) 547, 1105, 2058, 2505
Asp700I GAANNNNTTC 3 cut(s) 861, 1920, 2367
AspLEI GCGC 1 cut(s) 2163
AspS9I GGNCC 1 cut(s) 943
AvaII GGWCC 1 cut(s) 943
BaeGI GKGCMC 1 cut(s) 836
BalI TGGCCA 1 cut(s) 808
BbrPI CACGTG 1 cut(s) 150
BbsI GAAGAC 3 cut(s) 1587, 1875, 2322
Bbv12I GWGCWC 1 cut(s) 836
BbvI GCAGC 4 cut(s) 559, 1117, 2045, 2492
BccI CCATC 8 cut(s) 29, 265, 961, 1024, 1208, 2321, 2965, 3036
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 3 cut(s) 57, 640, 1453
BcoDI GTCTC 5 cut(s) 1356, 1592, 2111, 2558, 2686
BfmI CTRYAG 2 cut(s) 177, 207
BfrI CTTAAG 1 cut(s) 418
BfuI GTATCC 3 cut(s) 57, 640, 1453
BglII AGATCT 1 cut(s) 1475
BisI GCNGC 4 cut(s) 548, 1106, 2059, 2506
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 4 cut(s) 549, 1107, 2060, 2507
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 943
BmgBI CACGTC 1 cut(s) 2665
BmgT120I GGNCC 1 cut(s) 943
BmiI GGNNCC 1 cut(s) 3047
BmrFI CCNGG 1 cut(s) 61
BmrI ACTGGG 1 cut(s) 1109
BmsI GCATC 5 cut(s) 759, 963, 1441, 1459, 2637
BmuI ACTGGG 1 cut(s) 1109
BpiI GAAGAC 3 cut(s) 1587, 1875, 2322
BpmI CTGGAG 2 cut(s) 1283, 2450
Bpu1102I GCTNAGC 1 cut(s) 741
BpuEI CTTGAG 4 cut(s) 1241, 1511, 1553, 2681
BsaAI YACGTR 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 414
BsaI GGTCTC 2 cut(s) 2111, 2558
BsaJI CCNNGG 5 cut(s) 1481, 1590, 1776, 2223, 2901
BsaWI WCCGGW 1 cut(s) 533
BsaXI ACNNNNNCTCC 4 cut(s) 1397, 1427, 2854, 2884
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 3 cut(s) 1104, 1266, 2778
Bse3DI GCAATG 1 cut(s) 1019
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 5 cut(s) 1481, 1590, 1776, 2223, 2901
BseMI GCAATG 1 cut(s) 1019
BseMII CTCAG 3 cut(s) 690, 1367, 2444
BseNI ACTGG 3 cut(s) 1104, 1266, 2778
BseRI GAGGAG 1 cut(s) 2862
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 4 cut(s) 559, 1117, 2045, 2492
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 2 cut(s) 275, 808
BsiHKAI GWGCWC 1 cut(s) 836
BsiSI CCGG 2 cut(s) 276, 534
BslFI GGGAC 1 cut(s) 144
BsmAI GTCTC 5 cut(s) 1356, 1592, 2111, 2558, 2686
BsmBI CGTCTC 1 cut(s) 1592
BsmFI GGGAC 1 cut(s) 144
BsmI GAATGC 5 cut(s) 643, 720, 896, 1633, 2773
BsnI GGCC 2 cut(s) 275, 808
Bso31I GGTCTC 2 cut(s) 2111, 2558
Bsp1286I GDGCHC 1 cut(s) 836
Bsp13I TCCGGA 1 cut(s) 533
Bsp1407I TGTACA 1 cut(s) 1792
Bsp143I GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
Bsp1720I GCTNAGC 1 cut(s) 741
Bsp19I CCATGG 3 cut(s) 1481, 1776, 2223
BspACI CCGC 4 cut(s) 1818, 1863, 2265, 2744
BspANI GGCC 2 cut(s) 275, 808
BspCNI CTCAG 3 cut(s) 691, 1366, 2445
BspEI TCCGGA 1 cut(s) 533
BspLI GGNNCC 1 cut(s) 3047
BspMAI CTGCAG 2 cut(s) 181, 211
BspPI GGATC 4 cut(s) 313, 768, 848, 2087
BspQI GCTCTTC 3 cut(s) 34, 1401, 2418
BspTI CTTAAG 1 cut(s) 418
BspTNI GGTCTC 2 cut(s) 2111, 2558
BsrDI GCAATG 1 cut(s) 1019
BsrFI RCCGGY 1 cut(s) 275
BsrGI TGTACA 1 cut(s) 1792
BsrI ACTGG 3 cut(s) 1104, 1266, 2778
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 5 cut(s) 1481, 1590, 1776, 2223, 2901
BssMI GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
BssNAI GTATAC 1 cut(s) 1013
BssNI GRCGYC 1 cut(s) 414
BssT1I CCWWGG 4 cut(s) 1481, 1590, 1776, 2223
Bst1107I GTATAC 1 cut(s) 1013
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 8 cut(s) 31, 367, 519, 692, 1240, 2122, 2569, 2952
Bst6I CTCTTC 9 cut(s) 34, 1038, 1188, 1401, 2067, 2082, 2088, 2418, 2529
BstACI GRCGYC 1 cut(s) 414
BstAFI CTTAAG 1 cut(s) 418
BstAPI GCANNNNNTGC 1 cut(s) 638
BstAUI TGTACA 1 cut(s) 1792
BstBAI YACGTR 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 1327
BstDEI CTNAG 7 cut(s) 699, 741, 1109, 1127, 1353, 1720, 2453
BstDSI CCRYGG 4 cut(s) 1481, 1776, 2223, 2901
BstHHI GCGC 1 cut(s) 2163
BstKTI GATC 6 cut(s) 321, 724, 763, 843, 1478, 2082
BstMAI GTCTC 5 cut(s) 1356, 1592, 2111, 2558, 2686
BstMBI GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
BstMWI GCNNNNNNNGC 4 cut(s) 470, 638, 661, 1400
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 5 cut(s) 171, 1009, 1888, 2271, 2335
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 2 cut(s) 177, 207
BstSLI GKGCMC 1 cut(s) 836
BstV1I GCAGC 4 cut(s) 559, 1117, 2045, 2492
BstV2I GAAGAC 3 cut(s) 1587, 1875, 2322
BstX2I RGATCY 3 cut(s) 840, 1475, 2079
BstYI RGATCY 3 cut(s) 840, 1475, 2079
BstZ17I GTATAC 1 cut(s) 1013
BsuI GTATCC 3 cut(s) 57, 640, 1453
BsuRI GGCC 2 cut(s) 275, 808
BtgI CCRYGG 4 cut(s) 1481, 1776, 2223, 2901
BtgZI GCGATG 1 cut(s) 1227
BtrI CACGTC 1 cut(s) 2665
BtsI GCAGTG 2 cut(s) 650, 2317
BtsIMutI CAGTG 7 cut(s) 650, 697, 973, 1740, 2317, 2487, 2771
Cac8I GCNNGC 1 cut(s) 1327
CfoI GCGC 1 cut(s) 2163
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 1 cut(s) 943
CseI GACGC 2 cut(s) 422, 1400
Csp6I GTAC 2 cut(s) 1039, 1793
CviQI GTAC 2 cut(s) 1039, 1793
DdeI CTNAG 7 cut(s) 699, 741, 1109, 1127, 1353, 1720, 2453
DpnI GATC 6 cut(s) 320, 723, 762, 842, 1477, 2081
DpnII GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
DraI TTTAAA 2 cut(s) 1755, 2341
EaeI YGGCCR 2 cut(s) 273, 806
Eam1104I CTCTTC 9 cut(s) 34, 1038, 1188, 1401, 2067, 2082, 2088, 2418, 2529
EarI CTCTTC 9 cut(s) 34, 1038, 1188, 1401, 2067, 2082, 2088, 2418, 2529
EciI GGCGGA 2 cut(s) 1807, 2733
Eco130I CCWWGG 4 cut(s) 1481, 1590, 1776, 2223
Eco31I GGTCTC 2 cut(s) 2111, 2558
Eco32I GATATC 1 cut(s) 2262
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 1 cut(s) 1599
Eco72I CACGTG 1 cut(s) 150
EcoRI GAATTC 1 cut(s) 1573
EcoRII CCWGG 1 cut(s) 59
EcoRV GATATC 1 cut(s) 2262
EcoT14I CCWWGG 4 cut(s) 1481, 1590, 1776, 2223
EcoT22I ATGCAT 3 cut(s) 643, 720, 1456
ErhI CCWWGG 4 cut(s) 1481, 1590, 1776, 2223
Esp3I CGTCTC 1 cut(s) 1592
FalI AAGNNNNNCTT 4 cut(s) 2085, 2117, 2532, 2564
FaqI GGGAC 1 cut(s) 144
FblI GTMKAC 1 cut(s) 1012
Fnu4HI GCNGC 4 cut(s) 548, 1106, 2059, 2506
Fsp4HI GCNGC 4 cut(s) 548, 1106, 2059, 2506
FspI TGCGCA 1 cut(s) 2162
GlaI GCGC 1 cut(s) 2162
GluI GCNGC 4 cut(s) 548, 1106, 2059, 2506
GsuI CTGGAG 2 cut(s) 1283, 2450
HaeIII GGCC 2 cut(s) 275, 808
HapII CCGG 2 cut(s) 276, 534
HgaI GACGC 2 cut(s) 422, 1400
HhaI GCGC 1 cut(s) 2163
Hin1I GRCGYC 1 cut(s) 414
Hin6I GCGC 1 cut(s) 2161
HinP1I GCGC 1 cut(s) 2161
HincII GTYRAC 2 cut(s) 1390, 1696
HindII GTYRAC 2 cut(s) 1390, 1696
HindIII AAGCTT 3 cut(s) 662, 1910, 2357
HpaII CCGG 2 cut(s) 276, 534
Hpy166II GTNNAC 7 cut(s) 606, 834, 1013, 1390, 1696, 1826, 2273
Hpy188I TCNGA 7 cut(s) 713, 768, 1207, 1336, 1383, 1579, 2454
Hpy8I GTNNAC 7 cut(s) 606, 834, 1013, 1390, 1696, 1826, 2273
HpyCH4III ACNGT 8 cut(s) 31, 367, 519, 692, 1240, 2122, 2569, 2952
HpyCH4IV ACGT 3 cut(s) 149, 1058, 2664
HpyF10VI GCNNNNNNNGC 4 cut(s) 470, 638, 661, 1400
HpyF3I CTNAG 7 cut(s) 699, 741, 1109, 1127, 1353, 1720, 2453
HpySE526I ACGT 3 cut(s) 149, 1058, 2664
Hsp92I GRCGYC 1 cut(s) 414
HspAI GCGC 1 cut(s) 2161
Kpn2I TCCGGA 1 cut(s) 533
Kzo9I GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
LguI GCTCTTC 3 cut(s) 34, 1401, 2418
LmnI GCTCC 2 cut(s) 508, 1048
Lsp1109I GCAGC 4 cut(s) 559, 1117, 2045, 2492
LweI GCATC 5 cut(s) 759, 963, 1441, 1459, 2637
MaeII ACGT 3 cut(s) 149, 1058, 2664
MaeIII GTNAC 6 cut(s) 163, 278, 361, 2720, 2833, 2893
MalI GATC 6 cut(s) 320, 723, 762, 842, 1477, 2081
MboI GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
MfeI CAATTG 1 cut(s) 1175
MflI RGATCY 3 cut(s) 840, 1475, 2079
MhlI GDGCHC 1 cut(s) 836
MlsI TGGCCA 1 cut(s) 808
MluNI TGGCCA 1 cut(s) 808
MlyI GAGTC 4 cut(s) 55, 932, 1174, 2688
MmeI TCCRAC 6 cut(s) 981, 1129, 1176, 1440, 1684, 2692
Mox20I TGGCCA 1 cut(s) 808
Mph1103I ATGCAT 3 cut(s) 643, 720, 1456
MroI TCCGGA 1 cut(s) 533
MroXI GAANNNNTTC 3 cut(s) 861, 1920, 2367
MscI TGGCCA 1 cut(s) 808
MslI CAYNNNNRTG 3 cut(s) 561, 1743, 2190
Msp20I TGGCCA 1 cut(s) 808
MspCI CTTAAG 1 cut(s) 418
MspI CCGG 2 cut(s) 276, 534
MspR9I CCNGG 1 cut(s) 61
MunI CAATTG 1 cut(s) 1175
Mva1269I GAATGC 5 cut(s) 643, 720, 896, 1633, 2773
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 4 cut(s) 470, 638, 661, 1400
NcoI CCATGG 3 cut(s) 1481, 1776, 2223
NdeII GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
NlaIV GGNNCC 1 cut(s) 3047
NmuCI GTSAC 4 cut(s) 163, 278, 361, 2720
NsbI TGCGCA 1 cut(s) 2162
NsiI ATGCAT 3 cut(s) 643, 720, 1456
NspI RCATGY 5 cut(s) 171, 1009, 1888, 2271, 2335
PciI ACATGT 3 cut(s) 167, 1005, 1884
PciSI GCTCTTC 3 cut(s) 34, 1401, 2418
PcsI WCGNNNNNNNCGW 1 cut(s) 2151
PctI GAATGC 5 cut(s) 643, 720, 896, 1633, 2773
PdmI GAANNNNTTC 3 cut(s) 861, 1920, 2367
PfeI GAWTC 6 cut(s) 79, 537, 1438, 1938, 2385, 2914
PflFI GACNNNGTC 1 cut(s) 941
PkrI GCNGC 4 cut(s) 549, 1107, 2060, 2507
PleI GAGTC 4 cut(s) 54, 932, 1174, 2688
PmaCI CACGTG 1 cut(s) 150
PmlI CACGTG 1 cut(s) 150
PpsI GAGTC 4 cut(s) 54, 932, 1174, 2688
Ppu21I YACGTR 1 cut(s) 150
PscI ACATGT 3 cut(s) 167, 1005, 1884
PsiI TTATAA 2 cut(s) 489, 1893
Psp1406I AACGTT 1 cut(s) 1058
Psp6I CCWGG 1 cut(s) 59
PspCI CACGTG 1 cut(s) 150
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 3047
PspPI GGNCC 1 cut(s) 943
PstI CTGCAG 2 cut(s) 181, 211
PsuI RGATCY 3 cut(s) 840, 1475, 2079
PsyI GACNNNGTC 1 cut(s) 941
RsaI GTAC 2 cut(s) 1040, 1794
RsaNI GTAC 2 cut(s) 1039, 1793
RseI CAYNNNNRTG 3 cut(s) 561, 1743, 2190
SapI GCTCTTC 3 cut(s) 34, 1401, 2418
SatI GCNGC 4 cut(s) 548, 1106, 2059, 2506
Sau3AI GATC 6 cut(s) 318, 721, 760, 840, 1475, 2079
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 4 cut(s) 55, 932, 1174, 2688
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 836
SfaNI GCATC 5 cut(s) 759, 963, 1441, 1459, 2637
SfcI CTRYAG 2 cut(s) 177, 207
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 3 cut(s) 561, 1743, 2190
SmlI CTYRAG 5 cut(s) 418, 1220, 1526, 1568, 2696
SmoI CTYRAG 5 cut(s) 418, 1220, 1526, 1568, 2696
SsiI CCGC 4 cut(s) 1818, 1863, 2265, 2744
SspI AATATT 1 cut(s) 862
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 4 cut(s) 1481, 1590, 1776, 2223
TaaI ACNGT 8 cut(s) 31, 367, 519, 692, 1240, 2122, 2569, 2952
TaiI ACGT 3 cut(s) 152, 1061, 2667
TaqI TCGA 1 cut(s) 2631
TaqII GACCGA 1 cut(s) 931
TatI WGTACW 2 cut(s) 1038, 1792
TfiI GAWTC 6 cut(s) 79, 537, 1438, 1938, 2385, 2914
TscAI CASTG 7 cut(s) 657, 697, 973, 1740, 2317, 2487, 2778
TseFI GTSAC 4 cut(s) 163, 278, 361, 2720
TseI GCWGC 4 cut(s) 547, 1105, 2058, 2505
Tsp45I GTSAC 4 cut(s) 163, 278, 361, 2720
TspDTI ATGAA 5 cut(s) 33, 261, 1430, 1563, 2452
TspGWI ACGGA 6 cut(s) 470, 649, 1616, 1892, 2403, 2890
TspRI CASTG 7 cut(s) 657, 697, 973, 1740, 2317, 2487, 2778
Tth111I GACNNNGTC 1 cut(s) 941
Vha464I CTTAAG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 943
XceI RCATGY 5 cut(s) 171, 1009, 1888, 2271, 2335
XmiI GTMKAC 1 cut(s) 1012
XmnI GAANNNNTTC 3 cut(s) 861, 1920, 2367
Zsp2I ATGCAT 3 cut(s) 643, 720, 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.