Rh4AG084000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000042
Physical Location & Seq
Reverse (-)
17523962 .. 17531494
7533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG084000.1

Sequence Viewer

Length: 4023 bp
ATGTGGTTTCTTGCTGCAATTACTGGAGTTGTTGGAAAGATTGCTGAGTTCACAGTTCCACCAGTTATACGCCAAGTGGGTTATGTAATCCACTACAACAAGAACCTCAAAAAACTAGAGAGTCGAGTAGATGACTTGGGTGAGGCCAGAGAAAGAATGAAGCATGCTGTTGAAACAGAGAAAAGAAAAGGTAGAGAAATTGAAGCAGGTGTCCAGAACTGGCTGACTAAAGTGGATGAGATGATTGAAAAAGCAAACAATTTCTTGAAAGATGGACAGCATGCAGAGACTAGATGTCTCCATGGCTTTTGTCCTAATCTAAAGCTTCGCCATCGTCGCAGCAGGAAATCAACAAAATTGGTTAAAGAAGTTGCTGAGCTCTGTGGGAAAAAGGATTTTGGCGATATTGCATACGATGTCGAACCAGAGGAGGTGTGCAGCACACCTGCTAAAGATTACCAGGCCTTTGATTCAAGGACTTCGGTTGCGAAGGAAATCATGAATGAACTGAGACAATCTGATACCCACATAATTGGGGTGTACGGTATTGGGGGTGTGGGGAAGACAACGCTTGCTAAAGAAGTTTATCGGCAAGCCACAGAAGATAAAACTTTATTTGATGATGTTGTTATAGTAGAAGATGTGAAAAAGATTCCAGATTTGCATGGAATTCAAAAAAGAATTACTGAAAAGTTGGGAATGGACTTTCTCGGAGATGAGACTACAGACAAAAGAGCTAGTCGTCTTTTTAGTAGGATAAAAAAGAATAAAGTTCTGGTAATTTTGGATGACATACATGCAAAAATAGATTTGGAGATTGTGGGAGTTCCACGTGTGTCGAATTGTAAAGTATTGTTGACATCTAGAACTAGAGAAGTATTATCGGGTGAGATGCGTACACAAAAGGAGTTTCAACTTGACCTTTTAGATGCAAAAGAAAATTGGAGTTTATTTGAGAAGATGGCAGGTGGTGATGTTCAAAATCCTGCCATACGAGAAGTAGCAATAGAAGTAGCCCAAAAGTGTGGAGGCTTGCCGCTTTTAGTGGTAACAGTTGCAAGTGCCCTGAAGGATAGAAGTGCATTATATGCTTGGAAAGATGCCGTGCGACGCCTAAAAATATTTGATAAAGAAGGATTCACGGAAAAATCATACTTGGCTCTGGAGTGGAGTTACGAGCAATTGGATGATAAGGAACTTAAGCCATTGTGCTTGCTATCTGGAATTATGAGGGATTACTCTATGAATCTTCTTGACTTGCTGAAATACAGTATGGGCTTAGGTTTGATTAAAAACGTGAATACGATGGAAGAAGCACAGGATGCACTACATTCACTGGTTGAAAAACTTAAAGATTCTTGTCTATTGCTAGAAGACAGTGATAATAATAAATATTTTAGAATGCATGATCTTGTTCATGAAGTTGTAAACCAGATTGCCTTGAGAGAGCAACATGTCTTCAAAGTTACTATTGGAGATGAGTTCAAAGAATGGCCAGCTAAGGACTTTTGTAAAAAGAGCACTATGATCATTCTTGATAACTGCAGTATTCCCCGTCTTCCTGAAGGTTTGGAATACCCAGAACTGGAAATGTTTACTTTTTGTAGTAGCGATCTTGATCACTGCTTGGAAATTCCCAGAAACTTTTTTGAAGGGATGAAGAAACTCAAAGTGTTGGACTTCACTAATTTGAGTATCCCAACGCTACCTCTATCTCTCCAATTCCTAAAAAGTCTTCGGACATTGTGTTTGGATGCCTGCACCTTGGGAGACCTAGCTTTAGTTGGAGAGCTAAGAAACTTAGAAATGCTTAGCTTTTTAAAATCCAAGTTTAGAAAGTTGCCCGAAGAAATAGGGCAATTGACACGTCTGCGATTGTTGGATTTGAATGGTTGCTCTCAGCTCGAAGTGATTTCACCTAATGTTATATCAAACTTGAAAAGGTTAGAAAACTTGAGAATGGGTGATAGCTTCAACCGATGGGAGGGTGAAGAAGTCATCAGTAGTCAAAGAAGAAATGCATACCTTGAAGAGCTAAAGCACTTGCCTCAGCTAACTGCTTTACAAATACATATCTCAGATGCTAACATCGTTCCAGCAGATTTGTTCACCACTAGTGAGTTAAAGAGATTCCAAATATGTATTGGTTCTGCATGGAAATGGGATAATGTGGATGAAGCCCTCAATGTACTAAAGCTCCGACTCACTGCTAGCAATGAATTGGACCAAGGTCTAAGAATGTTGCTAAAAAGAACTGAAGACTTGTACTTGGAGGGGACGGAGGCTGTTAATAATAGTATTGTCTATCAAATAGGTGTCGAAGGTTTTCAGAAATTGAAGCATCTCCATCTCCAAACGACTGCCGAGTTTTTGTATATCATAAATGGGAAGGTTAGGTATCCCAACTTGACATGGTTGGGGGTGAATGAACTCAATGGTTTAACATTCTTGTTATCATCTTCAATGGCTAGAAGCCTTGCACAACTCAAACGTCTTCAGGTATCAGGATGTCAAATAATGGAAGAGATAGTATCAATAGAAGAATCTGATGAAGAAATTGTAGACAACTTCTTTTGCCAGCTACAAATTCTAGAGCTAAAAGACCTTCCAAATCTCACTAAATTTTGCTCAAGAAACCATACTGAACTTTCAAATCCATCCTCTGGGATACAACACTTTCTTTTTGACAATAAGGTAGAGTTTCCAAACTTGAAGAAGTTATCCATTGATGGCCTAGCAAACTTGACGACAATATGGAACAATCAATTTTCTCTAGAGAGTTCAAAAAATCTAGAGATAATTGAAATTATTTCATGTGACAGTTTGAAAAGTATTTTTCCAGTTTTGGTGGCTAGAAATCTCCAGCAGTTAAAAAGGCTGATAGTGAAGAGTTGTGGAGCTCAGGAAATTGTTGCAAAGGAAGAGTCACTACAAACAACACCTAAGTTTGTGTTTCCAAAAGTAAAAGAAGTGATTTTTAGTGATATGCCACAACTCAAGAATTTCTACCTAGGAATGCATGTGTCTGGCTGGCCGTCTCTTAATGAGTTGACGGTGGTAGAATGTGCTAAAGTCAACGTGTTTGCCGAGGAAATCTCAAGTTTTCAAGAACATAATGAGCCGGAACGTCTTAGTGTTCTTATCTCGCAGTCTCTCTTTTTAATTGAGAAGGACTCATTTCCCAACTTGGAATGGTTGTTCGTGAGAGCAATGGAGCCACTCCCAGCAGCAGCAGCGCAATTGTTTAGCAAACTAAAGCATCTTGAGGTTTGTTGTCCGGAGTCCAAGTCACTTGTTTTCCTTGACAAATTACTCGACTCAGAAGGAAACAGCAATAGTAGTGCAGTTGGGATTGGGACCCTGCAGCTCCCACATTTAAAAGAGTTGTGGCTTCTTCGAATGGAGAAGCTGACGCATCTAGGCCAAGATGATGAAGGGGAAAACTTACAATCAGCCCCCCGCATCCCAAATTTTCCCAACTTGGAAATTCTATACGTGAATGGTTGCGACAGCTTAAGGAATCTTAGATCATCAGCAATCTCCTTCAACACCCTAACAACTTTGCAAGTAAGTGTTTGCAAGGGGCTGAAATACTTGATAACTTACTCCATGGCTAAAAGTTTAACACAGCTCACAGAATTGGAAGTTGTGGAGTGTAAGAGACTGGTATATATAGTGGCAAGCAATGAAGATGACGATTCCTTAGGAAATTATGAGATTACATTCAGGCGGCTGAAACATTTGAAACTATCAAGTCTACCAAGAATGCAAGGCTTTTGCTCCGGAAATTGCATTGCTAAATTCCCGTCCTTGAAAACTTCAAGCATGAGCAACCGCCTCAAATTGAAGATTTTCGAAGCTCATAACCAAACGCTACAGCTAACAGACGAAGAGGCAGACACAGATGTCGATTACTTCGAGGCAAGTCTGATTGCTGAGGATAAAAGGGATTCGGCAATTGTCTGGGGCTTAACTGGTAACTTAACTGGATCTGGTATGAGACAGTATCAGAGGGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1340

Amino Acids

152.25

Weight (kDa)

5.79

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 322 2e-27 NB-ARC domain
LRR_14 PF23598 553 - 786 1.3e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 798 - 882 3.6e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 900 - 966 4.1e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1052 - 1177 7.4e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1176 - 1275 7e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 197, 454, 956
AasI GACNNNNNNGTC 1 cut(s) 3905
Acc36I ACCTGC 3 cut(s) 197, 454, 956
AccB7I CCANNNNNTGG 1 cut(s) 2663
AccI GTMKAC 2 cut(s) 2562, 3757
AccIII TCCGGA 2 cut(s) 3277, 3782
AciI CCGC 4 cut(s) 1037, 3460, 3730, 3835
AclWI GGATC 1 cut(s) 3997
AcoI YGGCCR 2 cut(s) 1493, 3032
AcsI RAATTY 8 cut(s) 669, 1632, 2586, 2621, 3001, 3469, 3486, 3800
AcuI CTGAAG 4 cut(s) 1088, 1584, 2277, 2480
AcvI CACGTG 1 cut(s) 833
AcyI GRCGYC 1 cut(s) 1111
AfaI GTAC 4 cut(s) 542, 898, 2190, 2267
AfiI CCNNNNNNNGG 3 cut(s) 1585, 1984, 2663
AflII CTTAAG 2 cut(s) 1199, 3514
AflIII ACRYGT 4 cut(s) 832, 1453, 1865, 3078
AhdI GACNNNNNGTC 1 cut(s) 294
AhlI ACTAGT 1 cut(s) 2114
AjiI CACGTC 1 cut(s) 1868
AjnI CCWGG 1 cut(s) 459
AloI GAACNNNNNNTCC 2 cut(s) 3182, 3214
Alw21I GWGCWC 3 cut(s) 381, 1523, 2902
Alw26I GTCTC 9 cut(s) 281, 302, 505, 713, 1764, 3042, 3156, 3655, 3994
AlwI GGATC 1 cut(s) 3997
Aor13HI TCCGGA 2 cut(s) 3277, 3782
AoxI GGCC 6 cut(s) 144, 462, 1493, 2731, 3032, 3421
ApeKI GCWGC 7 cut(s) 14, 339, 438, 3227, 3230, 3233, 3364
ApoI RAATTY 8 cut(s) 669, 1632, 2586, 2621, 3001, 3469, 3486, 3800
ArsI GACNNNNNNTTYG 4 cut(s) 1732, 1764, 2909, 2941
Asp700I GAANNNNTTC 2 cut(s) 2325, 3851
AspA2I CCTAGG 1 cut(s) 3010
AspLEI GCGC 1 cut(s) 3238
AspS9I GGNCC 2 cut(s) 2224, 3357
AsuHPI GGTGA 8 cut(s) 152, 899, 983, 1908, 1976, 2000, 2101, 2434
AsuII TTCGAA 2 cut(s) 3397, 3855
AsuNHI GCTAGC 1 cut(s) 2210
AvaII GGWCC 2 cut(s) 2224, 3357
AvrII CCTAGG 1 cut(s) 3010
AxyI CCTNAGG 1 cut(s) 3703
BaeGI GKGCMC 1 cut(s) 1066
BalI TGGCCA 1 cut(s) 1495
BanII GRGCYC 2 cut(s) 381, 2902
BarI GAAGNNNNNNTAC 2 cut(s) 2913, 2945
BbrPI CACGTG 1 cut(s) 833
BbsI GAAGAC 7 cut(s) 569, 1380, 1450, 1550, 1727, 2265, 2486
Bbv12I GWGCWC 3 cut(s) 381, 1523, 2902
BbvCI CCTCAGC 2 cut(s) 2049, 3936
BbvI GCAGC 6 cut(s) 351, 450, 3239, 3242, 3245, 3376
BccI CCATC 8 cut(s) 266, 339, 955, 1300, 1974, 2355, 2665, 2723
BceAI ACGGC 2 cut(s) 1088, 3019
BciT130I CCWGG 1 cut(s) 461
BciVI GTATCC 3 cut(s) 1706, 2409, 2661
BclI TGATCA 2 cut(s) 1527, 1618
BcoDI GTCTC 9 cut(s) 281, 302, 505, 713, 1764, 3042, 3156, 3655, 3994
BcuI ACTAGT 1 cut(s) 2114
BfmI CTRYAG 4 cut(s) 723, 1543, 3362, 3875
BfrI CTTAAG 2 cut(s) 1199, 3514
BfuAI ACCTGC 3 cut(s) 197, 454, 956
BfuI GTATCC 3 cut(s) 1706, 2409, 2661
BisI GCNGC 9 cut(s) 15, 340, 439, 1037, 3228, 3231, 3234, 3365, 3731
BlnI CCTAGG 1 cut(s) 3010
BlpI GCTNAGC 2 cut(s) 375, 1811
BlsI GCNGC 9 cut(s) 16, 341, 440, 1038, 3229, 3232, 3235, 3366, 3732
Bme1390I CCNGG 1 cut(s) 461
Bme18I GGWCC 2 cut(s) 2224, 3357
BmeRI GACNNNNNGTC 1 cut(s) 294
BmgBI CACGTC 1 cut(s) 1868
BmgT120I GGNCC 2 cut(s) 2224, 3357
BmiI GGNNCC 3 cut(s) 3216, 3358, 3359
BmrFI CCNGG 1 cut(s) 461
BmtI GCTAGC 1 cut(s) 2214
BoxI GACNNNNGTC 1 cut(s) 2229
BpiI GAAGAC 7 cut(s) 569, 1380, 1450, 1550, 1727, 2265, 2486
BplI GAGNNNNNCTC 4 cut(s) 3080, 3112, 3158, 3190
BpmI CTGGAG 3 cut(s) 45, 1184, 2846
Bpu10I CCTNAGC 5 cut(s) 1279, 1500, 2049, 2901, 3936
Bpu1102I GCTNAGC 2 cut(s) 375, 1811
Bpu14I TTCGAA 2 cut(s) 3397, 3855
BpuEI CTTGAG 6 cut(s) 1462, 1975, 2614, 2981, 3082, 3284
BsaAI YACGTR 2 cut(s) 833, 3496
BsaBI GATNNNNATC 1 cut(s) 1617
BsaHI GRCGYC 1 cut(s) 1111
BsaI GGTCTC 1 cut(s) 1764
BsaJI CCNNGG 6 cut(s) 301, 1764, 2227, 3010, 3087, 3609
BsaWI WCCGGW 2 cut(s) 3277, 3782
BsaXI ACNNNNNCTCC 4 cut(s) 2181, 2211, 3272, 3302
Bsc4I CCNNNNNNNGG 3 cut(s) 1585, 1984, 2663
Bse1I ACTGG 9 cut(s) 28, 62, 224, 1341, 1590, 2840, 3669, 3979, 3991
Bse21I CCTNAGG 1 cut(s) 3703
Bse3DI GCAATG 4 cut(s) 2221, 3216, 3691, 3792
Bse8I GATNNNNATC 1 cut(s) 1617
BseAI TCCGGA 2 cut(s) 3277, 3782
BseBI CCWGG 1 cut(s) 461
BseDI CCNNGG 6 cut(s) 301, 1764, 2227, 3010, 3087, 3609
BseJI GATNNNNATC 1 cut(s) 1617
BseLI CCNNNNNNNGG 3 cut(s) 1585, 1984, 2663
BseMI GCAATG 4 cut(s) 2221, 3216, 3691, 3792
BseMII CTCAG 9 cut(s) 36, 366, 500, 1913, 2063, 2091, 2915, 3333, 3927
BseNI ACTGG 9 cut(s) 28, 62, 224, 1341, 1590, 2840, 3669, 3979, 3991
BseRI GAGGAG 1 cut(s) 443
BseSI GKGCMC 1 cut(s) 1066
BseXI GCAGC 6 cut(s) 351, 450, 3239, 3242, 3245, 3376
BseYI CCCAGC 1 cut(s) 3223
BsgI GTGCAG 3 cut(s) 457, 1744, 3363
BshFI GGCC 6 cut(s) 146, 464, 1495, 2733, 3034, 3423
BsiHKAI GWGCWC 3 cut(s) 381, 1523, 2902
BsiSI CCGG 3 cut(s) 3122, 3278, 3783
BslFI GGGAC 2 cut(s) 2290, 3370
BslI CCNNNNNNNGG 3 cut(s) 1585, 1984, 2663
BsmAI GTCTC 9 cut(s) 281, 302, 505, 713, 1764, 3042, 3156, 3655, 3994
BsmBI CGTCTC 1 cut(s) 3042
BsmFI GGGAC 2 cut(s) 2290, 3370
BsmI GAATGC 3 cut(s) 1407, 3021, 3771
BsnI GGCC 6 cut(s) 146, 464, 1495, 2733, 3034, 3423
Bso31I GGTCTC 1 cut(s) 1764
Bsp119I TTCGAA 2 cut(s) 3397, 3855
Bsp1286I GDGCHC 4 cut(s) 381, 1066, 1523, 2902
Bsp13I TCCGGA 2 cut(s) 3277, 3782
Bsp143I GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
Bsp1720I GCTNAGC 2 cut(s) 375, 1811
Bsp19I CCATGG 2 cut(s) 301, 3609
BspACI CCGC 4 cut(s) 1037, 3460, 3730, 3835
BspANI GGCC 6 cut(s) 146, 464, 1495, 2733, 3034, 3423
BspCNI CTCAG 9 cut(s) 37, 367, 501, 1912, 2062, 2090, 2914, 3332, 3928
BspEI TCCGGA 2 cut(s) 3277, 3782
BspHI TCATGA 2 cut(s) 498, 1417
BspLI GGNNCC 3 cut(s) 3216, 3358, 3359
BspMAI CTGCAG 2 cut(s) 1547, 3366
BspMI ACCTGC 3 cut(s) 197, 454, 956
BspOI GCTAGC 1 cut(s) 2214
BspPI GGATC 1 cut(s) 3997
BspQI GCTCTTC 1 cut(s) 2025
BspT104I TTCGAA 2 cut(s) 3397, 3855
BspTI CTTAAG 2 cut(s) 1199, 3514
BspTNI GGTCTC 1 cut(s) 1764
BsrDI GCAATG 4 cut(s) 2221, 3216, 3691, 3792
BsrI ACTGG 9 cut(s) 28, 62, 224, 1341, 1590, 2840, 3669, 3979, 3991
BssECI CCNNGG 6 cut(s) 301, 1764, 2227, 3010, 3087, 3609
BssMI GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
BssNI GRCGYC 1 cut(s) 1111
BssT1I CCWWGG 5 cut(s) 301, 1764, 2227, 3010, 3609
Bst2UI CCWGG 1 cut(s) 461
Bst4CI ACNGT 8 cut(s) 55, 545, 1054, 1271, 1379, 2822, 3055, 4005
Bst6I CTCTTC 5 cut(s) 2025, 2517, 2882, 2916, 3885
BstACI GRCGYC 1 cut(s) 1111
BstAFI CTTAAG 2 cut(s) 1199, 3514
BstAPI GCANNNNNTGC 2 cut(s) 1088, 1322
BstBAI YACGTR 2 cut(s) 833, 3496
BstBI TTCGAA 2 cut(s) 3397, 3855
BstDSI CCRYGG 2 cut(s) 301, 3609
BstHHI GCGC 1 cut(s) 3238
BstKTI GATC 6 cut(s) 1411, 1530, 1615, 1621, 3530, 3992
BstMAI GTCTC 9 cut(s) 281, 302, 505, 713, 1764, 3042, 3156, 3655, 3994
BstMBI GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
BstMWI GCNNNNNNNGC 4 cut(s) 336, 1088, 1322, 3233
BstNI CCWGG 1 cut(s) 461
BstNSI RCATGY 5 cut(s) 167, 284, 800, 1457, 3023
BstPAI GACNNNNGTC 1 cut(s) 2229
BstSCI CCNGG 1 cut(s) 459
BstSFI CTRYAG 4 cut(s) 723, 1543, 3362, 3875
BstSLI GKGCMC 1 cut(s) 1066
BstV1I GCAGC 6 cut(s) 351, 450, 3239, 3242, 3245, 3376
BstV2I GAAGAC 7 cut(s) 569, 1380, 1450, 1550, 1727, 2265, 2486
BstX2I RGATCY 1 cut(s) 3989
BstXI CCANNNNNNTGG 2 cut(s) 533, 1025
BstYI RGATCY 1 cut(s) 3989
Bsu36I CCTNAGG 1 cut(s) 3703
BsuI GTATCC 3 cut(s) 1706, 2409, 2661
BsuRI GGCC 6 cut(s) 146, 464, 1495, 2733, 3034, 3423
BtgI CCRYGG 2 cut(s) 301, 3609
BtrI CACGTC 1 cut(s) 1868
BtsI GCAGTG 2 cut(s) 1621, 2205
BtsIMutI CAGTG 4 cut(s) 1334, 1384, 1621, 2205
BveI ACCTGC 3 cut(s) 197, 454, 956
CciI TCATGA 2 cut(s) 498, 1417
CfoI GCGC 1 cut(s) 3238
Cfr13I GGNCC 2 cut(s) 2224, 3357
CseI GACGC 2 cut(s) 1119, 3421
Csp6I GTAC 4 cut(s) 541, 897, 2189, 2266
CviQI GTAC 4 cut(s) 541, 897, 2189, 2266
DpnI GATC 6 cut(s) 1410, 1529, 1614, 1620, 3529, 3991
DpnII GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
DraI TTTAAA 2 cut(s) 1821, 3378
DrdI GACNNNNNNGTC 1 cut(s) 3905
DriI GACNNNNNGTC 1 cut(s) 294
DseDI GACNNNNNNGTC 1 cut(s) 3905
EaeI YGGCCR 2 cut(s) 1493, 3032
Eam1104I CTCTTC 5 cut(s) 2025, 2517, 2882, 2916, 3885
Eam1105I GACNNNNNGTC 1 cut(s) 294
EarI CTCTTC 5 cut(s) 2025, 2517, 2882, 2916, 3885
Ecl136II GAGCTC 2 cut(s) 379, 2900
Eco130I CCWWGG 5 cut(s) 301, 1764, 2227, 3010, 3609
Eco147I AGGCCT 1 cut(s) 464
Eco24I GRGCYC 2 cut(s) 381, 2902
Eco31I GGTCTC 1 cut(s) 1764
Eco47I GGWCC 2 cut(s) 2224, 3357
Eco53kI GAGCTC 2 cut(s) 379, 2900
Eco57I CTGAAG 4 cut(s) 1088, 1584, 2277, 2480
Eco72I CACGTG 1 cut(s) 833
Eco81I CCTNAGG 1 cut(s) 3703
EcoICRI GAGCTC 2 cut(s) 379, 2900
EcoO109I RGGNCCY 1 cut(s) 3357
EcoRI GAATTC 1 cut(s) 669
EcoRII CCWGG 1 cut(s) 459
EcoT14I CCWWGG 5 cut(s) 301, 1764, 2227, 3010, 3609
EcoT22I ATGCAT 3 cut(s) 1407, 2023, 3021
EcoT38I GRGCYC 2 cut(s) 381, 2902
ErhI CCWWGG 5 cut(s) 301, 1764, 2227, 3010, 3609
Esp3I CGTCTC 1 cut(s) 3042
FaqI GGGAC 2 cut(s) 2290, 3370
FauI CCCGC 1 cut(s) 3467
FbaI TGATCA 2 cut(s) 1527, 1618
FblI GTMKAC 2 cut(s) 2562, 3757
Fnu4HI GCNGC 9 cut(s) 15, 340, 439, 1037, 3228, 3231, 3234, 3365, 3731
FriOI GRGCYC 2 cut(s) 381, 2902
Fsp4HI GCNGC 9 cut(s) 15, 340, 439, 1037, 3228, 3231, 3234, 3365, 3731
GlaI GCGC 1 cut(s) 3237
GluI GCNGC 9 cut(s) 15, 340, 439, 1037, 3228, 3231, 3234, 3365, 3731
GsaI CCCAGC 1 cut(s) 3227
GsuI CTGGAG 3 cut(s) 45, 1184, 2846
HaeIII GGCC 6 cut(s) 146, 464, 1495, 2733, 3034, 3423
HapII CCGG 3 cut(s) 3122, 3278, 3783
HgaI GACGC 2 cut(s) 1119, 3421
HhaI GCGC 1 cut(s) 3238
Hin1I GRCGYC 1 cut(s) 1111
Hin6I GCGC 1 cut(s) 3236
HinP1I GCGC 1 cut(s) 3236
HincII GTYRAC 3 cut(s) 858, 3051, 3076
HindII GTYRAC 3 cut(s) 858, 3051, 3076
HindIII AAGCTT 2 cut(s) 323, 4017
HpaII CCGG 3 cut(s) 3122, 3278, 3783
HphI GGTGA 8 cut(s) 152, 899, 983, 1908, 1976, 2000, 2101, 2434
Hpy99I CGWCG 2 cut(s) 339, 1113
HpyCH4III ACNGT 8 cut(s) 55, 545, 1054, 1271, 1379, 2822, 3055, 4005
HpyCH4IV ACGT 7 cut(s) 832, 1296, 1867, 2491, 3078, 3127, 3495
HpyF10VI GCNNNNNNNGC 4 cut(s) 336, 1088, 1322, 3233
HpySE526I ACGT 7 cut(s) 832, 1296, 1867, 2491, 3078, 3127, 3495
Hsp92I GRCGYC 1 cut(s) 1111
HspAI GCGC 1 cut(s) 3236
KflI GGGWCCC 1 cut(s) 3357
Kpn2I TCCGGA 2 cut(s) 3277, 3782
Ksp22I TGATCA 2 cut(s) 1527, 1618
Kzo9I GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
LguI GCTCTTC 1 cut(s) 2025
LmnI GCTCC 5 cut(s) 2202, 2897, 3214, 3372, 3785
Lsp1109I GCAGC 6 cut(s) 351, 450, 3239, 3242, 3245, 3376
MaeII ACGT 7 cut(s) 832, 1296, 1867, 2491, 3078, 3127, 3495
MaeIII GTNAC 7 cut(s) 1048, 1172, 1465, 2816, 2925, 3288, 3977
MalI GATC 6 cut(s) 1410, 1529, 1614, 1620, 3529, 3991
MboI GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
MfeI CAATTG 4 cut(s) 1181, 1859, 3239, 3957
MflI RGATCY 1 cut(s) 3989
MhlI GDGCHC 4 cut(s) 381, 1066, 1523, 2902
MlsI TGGCCA 1 cut(s) 1495
MluNI TGGCCA 1 cut(s) 1495
MlyI GAGTC 6 cut(s) 130, 2196, 2933, 3167, 3290, 3311
MmeI TCCRAC 5 cut(s) 13, 1656, 1765, 1860, 2224
Mox20I TGGCCA 1 cut(s) 1495
Mph1103I ATGCAT 3 cut(s) 1407, 2023, 3021
MroI TCCGGA 2 cut(s) 3277, 3782
MroXI GAANNNNTTC 2 cut(s) 2325, 3851
MscI TGGCCA 1 cut(s) 1495
MslI CAYNNNNRTG 1 cut(s) 2158
Msp20I TGGCCA 1 cut(s) 1495
MspCI CTTAAG 2 cut(s) 1199, 3514
MspI CCGG 3 cut(s) 3122, 3278, 3783
MspR9I CCNGG 1 cut(s) 461
MunI CAATTG 4 cut(s) 1181, 1859, 3239, 3957
Mva1269I GAATGC 3 cut(s) 1407, 3021, 3771
MvaI CCWGG 1 cut(s) 461
MwoI GCNNNNNNNGC 4 cut(s) 336, 1088, 1322, 3233
NcoI CCATGG 2 cut(s) 301, 3609
NdeII GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
NheI GCTAGC 1 cut(s) 2210
NlaIV GGNNCC 3 cut(s) 3216, 3358, 3359
NmeAIII GCCGAG 2 cut(s) 2389, 3112
NmuCI GTSAC 3 cut(s) 2816, 2925, 3288
NsiI ATGCAT 3 cut(s) 1407, 2023, 3021
NspI RCATGY 5 cut(s) 167, 284, 800, 1457, 3023
NspV TTCGAA 2 cut(s) 3397, 3855
PaeI GCATGC 2 cut(s) 167, 284
PagI TCATGA 2 cut(s) 498, 1417
PaqCI CACCTGC 3 cut(s) 197, 454, 956
PceI AGGCCT 1 cut(s) 464
PciI ACATGT 1 cut(s) 1453
PciSI GCTCTTC 1 cut(s) 2025
PcsI WCGNNNNNNNCGW 2 cut(s) 3084, 3915
PctI GAATGC 3 cut(s) 1407, 3021, 3771
PdmI GAANNNNTTC 2 cut(s) 2325, 3851
PflMI CCANNNNNTGG 1 cut(s) 2663
PkrI GCNGC 9 cut(s) 16, 341, 440, 1038, 3229, 3232, 3235, 3366, 3732
PleI GAGTC 6 cut(s) 129, 2196, 2932, 3167, 3289, 3311
PmaCI CACGTG 1 cut(s) 833
PmlI CACGTG 1 cut(s) 833
PpsI GAGTC 6 cut(s) 129, 2196, 2932, 3167, 3289, 3311
Ppu21I YACGTR 2 cut(s) 833, 3496
PpuMI RGGWCCY 1 cut(s) 3357
PscI ACATGT 1 cut(s) 1453
PshAI GACNNNNGTC 1 cut(s) 2229
Psp124BI GAGCTC 2 cut(s) 381, 2902
Psp5II RGGWCCY 1 cut(s) 3357
Psp6I CCWGG 1 cut(s) 459
PspCI CACGTG 1 cut(s) 833
PspFI CCCAGC 1 cut(s) 3223
PspGI CCWGG 1 cut(s) 459
PspN4I GGNNCC 3 cut(s) 3216, 3358, 3359
PspPI GGNCC 2 cut(s) 2224, 3357
PspPPI RGGWCCY 1 cut(s) 3357
PstI CTGCAG 2 cut(s) 1547, 3366
PsuI RGATCY 1 cut(s) 3989
RsaI GTAC 4 cut(s) 542, 898, 2190, 2267
RsaNI GTAC 4 cut(s) 541, 897, 2189, 2266
RseI CAYNNNNRTG 1 cut(s) 2158
SacI GAGCTC 2 cut(s) 381, 2902
SapI GCTCTTC 1 cut(s) 2025
SatI GCNGC 9 cut(s) 15, 340, 439, 1037, 3228, 3231, 3234, 3365, 3731
Sau3AI GATC 6 cut(s) 1408, 1527, 1612, 1618, 3527, 3989
Sau96I GGNCC 2 cut(s) 2224, 3357
SchI GAGTC 6 cut(s) 130, 2196, 2933, 3167, 3290, 3311
ScrFI CCNGG 1 cut(s) 461
SduI GDGCHC 4 cut(s) 381, 1066, 1523, 2902
SfcI CTRYAG 4 cut(s) 723, 1543, 3362, 3875
SfuI TTCGAA 2 cut(s) 3397, 3855
SinI GGWCC 2 cut(s) 2224, 3357
SmiMI CAYNNNNRTG 1 cut(s) 2158
SmlI CTYRAG 8 cut(s) 1199, 1441, 1954, 2629, 2996, 3097, 3263, 3514
SmoI CTYRAG 8 cut(s) 1199, 1441, 1954, 2629, 2996, 3097, 3263, 3514
SpeI ACTAGT 1 cut(s) 2114
SphI GCATGC 2 cut(s) 167, 284
SseBI AGGCCT 1 cut(s) 464
SsiI CCGC 4 cut(s) 1037, 3460, 3730, 3835
SspI AATATT 2 cut(s) 1122, 1394
SstI GAGCTC 2 cut(s) 381, 2902
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 1 cut(s) 459
StyI CCWWGG 5 cut(s) 301, 1764, 2227, 3010, 3609
TaaI ACNGT 8 cut(s) 55, 545, 1054, 1271, 1379, 2822, 3055, 4005
TaiI ACGT 7 cut(s) 835, 1299, 1870, 2494, 3081, 3130, 3498
TatI WGTACW 2 cut(s) 2188, 2265
TauI GCSGC 2 cut(s) 1039, 3733
TscAI CASTG 4 cut(s) 1341, 1384, 1628, 2212
TseFI GTSAC 3 cut(s) 2816, 2925, 3288
TseI GCWGC 7 cut(s) 14, 339, 438, 3227, 3230, 3233, 3364
Tsp45I GTSAC 3 cut(s) 2816, 2925, 3288
TspGWI ACGGA 2 cut(s) 1157, 2294
TspRI CASTG 4 cut(s) 1341, 1384, 1628, 2212
Van91I CCANNNNNTGG 1 cut(s) 2663
Vha464I CTTAAG 2 cut(s) 1199, 3514
VpaK11BI GGWCC 2 cut(s) 2224, 3357
XapI RAATTY 8 cut(s) 669, 1632, 2586, 2621, 3001, 3469, 3486, 3800
XbaI TCTAGA 4 cut(s) 863, 2590, 2773, 2791
XceI RCATGY 5 cut(s) 167, 284, 800, 1457, 3023
XcmI CCANNNNNNNNNTGG 1 cut(s) 2141
XmaJI CCTAGG 1 cut(s) 3010
XmiI GTMKAC 2 cut(s) 2562, 3757
XmnI GAANNNNTTC 2 cut(s) 2325, 3851
Zsp2I ATGCAT 3 cut(s) 1407, 2023, 3021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.