disease resistance
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1362 |
| AarI | CACCTGC | 1 cut(s) | 1139 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 1011, 1580 |
| Acc16I | TGCGCA | 1 cut(s) | 2663 |
| Acc36I | ACCTGC | 1 cut(s) | 1139 |
| AccB1I | GGYRCC | 1 cut(s) | 2584 |
| AccI | GTMKAC | 1 cut(s) | 235 |
| AciI | CCGC | 4 cut(s) | 62, 407, 588, 1618 |
| AclI | AACGTT | 1 cut(s) | 1931 |
| AclWI | GGATC | 3 cut(s) | 684, 1721, 2007 |
| AcoI | YGGCCR | 1 cut(s) | 1679 |
| AcsI | RAATTY | 7 cut(s) | 372, 569, 855, 1029, 1091, 1995, 2446 |
| AcuI | CTGAAG | 5 cut(s) | 369, 756, 858, 1822, 2234 |
| AcyI | GRCGYC | 2 cut(s) | 65, 244 |
| AfaI | GTAC | 1 cut(s) | 537 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 179, 1348 |
| AflIII | ACRYGT | 3 cut(s) | 1054, 1641, 2651 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 501 |
| AjnI | CCWGG | 2 cut(s) | 633, 1341 |
| Alw21I | GWGCWC | 1 cut(s) | 1709 |
| Alw26I | GTCTC | 4 cut(s) | 38, 2125, 2229, 2465 |
| Alw44I | GTGCAC | 1 cut(s) | 1705 |
| AlwI | GGATC | 3 cut(s) | 684, 1721, 2007 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 836 |
| Ama87I | CYCGRG | 1 cut(s) | 1775 |
| AoxI | GGCC | 1 cut(s) | 1679 |
| ApaLI | GTGCAC | 1 cut(s) | 1705 |
| ApeKI | GCWGC | 4 cut(s) | 317, 1947, 1978, 2603 |
| ApoI | RAATTY | 7 cut(s) | 372, 569, 855, 1029, 1091, 1995, 2446 |
| AseI | ATTAAT | 2 cut(s) | 882, 1229 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 372, 470, 843, 1734 |
| AspLEI | GCGC | 1 cut(s) | 2664 |
| AspS9I | GGNCC | 1 cut(s) | 2635 |
| AsuC2I | CCSGG | 1 cut(s) | 15 |
| AsuNHI | GCTAGC | 1 cut(s) | 1402 |
| AvaI | CYCGRG | 1 cut(s) | 1775 |
| AvaII | GGWCC | 1 cut(s) | 2635 |
| BaeGI | GKGCMC | 2 cut(s) | 934, 1709 |
| BalI | TGGCCA | 1 cut(s) | 1681 |
| BanI | GGYRCC | 1 cut(s) | 2584 |
| BbsI | GAAGAC | 2 cut(s) | 693, 2051 |
| Bbv12I | GWGCWC | 1 cut(s) | 1709 |
| BbvI | GCAGC | 4 cut(s) | 304, 1934, 1990, 2590 |
| BccI | CCATC | 5 cut(s) | 1671, 1834, 1840, 1897, 1937 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2207, 2241 |
| BciT130I | CCWGG | 2 cut(s) | 635, 1343 |
| BciVI | GTATCC | 1 cut(s) | 1513 |
| BcnI | CCSGG | 1 cut(s) | 15 |
| BcoDI | GTCTC | 4 cut(s) | 38, 2125, 2229, 2465 |
| BfaI | CTAG | 4 cut(s) | 1403, 1710, 1986, 2639 |
| BfmI | CTRYAG | 2 cut(s) | 903, 2235 |
| BfuAI | ACCTGC | 1 cut(s) | 1139 |
| BfuI | GTATCC | 1 cut(s) | 1513 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 326 |
| BglII | AGATCT | 1 cut(s) | 2348 |
| BisI | GCNGC | 5 cut(s) | 63, 318, 1948, 1979, 2604 |
| BlpI | GCTNAGC | 2 cut(s) | 512, 1614 |
| BlsI | GCNGC | 5 cut(s) | 64, 319, 1949, 1980, 2605 |
| Bme1390I | CCNGG | 3 cut(s) | 15, 635, 1343 |
| Bme18I | GGWCC | 1 cut(s) | 2635 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 501 |
| BmeT110I | CYCGRG | 1 cut(s) | 1775 |
| BmgT120I | GGNCC | 1 cut(s) | 2635 |
| BmiI | GGNNCC | 1 cut(s) | 2586 |
| BmrFI | CCNGG | 3 cut(s) | 15, 635, 1343 |
| BmrI | ACTGGG | 2 cut(s) | 248, 1982 |
| BmsI | GCATC | 7 cut(s) | 19, 642, 1065, 1276, 2254, 2314, 2323 |
| BmtI | GCTAGC | 1 cut(s) | 1406 |
| BmuI | ACTGGG | 2 cut(s) | 248, 1982 |
| BpiI | GAAGAC | 2 cut(s) | 693, 2051 |
| BpmI | CTGGAG | 3 cut(s) | 1680, 2472, 2516 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 512, 1614 |
| BpuEI | CTTGAG | 2 cut(s) | 282, 2384 |
| BpuMI | CCSGG | 1 cut(s) | 15 |
| Bsa29I | ATCGAT | 1 cut(s) | 129 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2613 |
| BsaHI | GRCGYC | 2 cut(s) | 65, 244 |
| BsaJI | CCNNGG | 4 cut(s) | 14, 475, 2354, 2580 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 2270, 2300 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 179, 1348 |
| Bse118I | RCCGGY | 1 cut(s) | 2632 |
| Bse1I | ACTGG | 5 cut(s) | 243, 388, 537, 1977, 2139 |
| Bse3DI | GCAATG | 1 cut(s) | 1892 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2613 |
| BseBI | CCWGG | 2 cut(s) | 635, 1343 |
| BseCI | ATCGAT | 1 cut(s) | 129 |
| BseDI | CCNNGG | 4 cut(s) | 14, 475, 2354, 2580 |
| BseGI | GGATG | 7 cut(s) | 10, 657, 973, 1682, 1826, 2329, 2338 |
| BseJI | GATNNNNATC | 1 cut(s) | 2613 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 179, 1348 |
| BseMI | GCAATG | 1 cut(s) | 1892 |
| BseNI | ACTGG | 5 cut(s) | 243, 388, 537, 1977, 2139 |
| BseRI | GAGGAG | 1 cut(s) | 22 |
| BseSI | GKGCMC | 2 cut(s) | 934, 1709 |
| BseXI | GCAGC | 4 cut(s) | 304, 1934, 1990, 2590 |
| BsgI | GTGCAG | 2 cut(s) | 405, 1101 |
| BshFI | GGCC | 1 cut(s) | 1681 |
| BshNI | GGYRCC | 1 cut(s) | 2584 |
| BshVI | ATCGAT | 1 cut(s) | 129 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1709 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1775 |
| BsiSI | CCGG | 3 cut(s) | 14, 1407, 2633 |
| BslFI | GGGAC | 2 cut(s) | 255, 1017 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 179, 1348 |
| BsmAI | GTCTC | 4 cut(s) | 38, 2125, 2229, 2465 |
| BsmBI | CGTCTC | 1 cut(s) | 38 |
| BsmFI | GGGAC | 2 cut(s) | 255, 1017 |
| BsmI | GAATGC | 4 cut(s) | 1516, 1593, 1769, 2506 |
| BsnI | GGCC | 1 cut(s) | 1681 |
| BsoBI | CYCGRG | 1 cut(s) | 1775 |
| Bsp1286I | GDGCHC | 2 cut(s) | 934, 1709 |
| Bsp143I | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 512, 1614 |
| Bsp19I | CCATGG | 2 cut(s) | 475, 2354 |
| BspACI | CCGC | 4 cut(s) | 62, 407, 588, 1618 |
| BspANI | GGCC | 1 cut(s) | 1681 |
| BspDI | ATCGAT | 1 cut(s) | 129 |
| BspHI | TCATGA | 2 cut(s) | 117, 445 |
| BspLI | GGNNCC | 1 cut(s) | 2586 |
| BspMAI | CTGCAG | 1 cut(s) | 2239 |
| BspMI | ACCTGC | 1 cut(s) | 1139 |
| BspOI | GCTAGC | 1 cut(s) | 1406 |
| BspPI | GGATC | 3 cut(s) | 684, 1721, 2007 |
| BspQI | GCTCTTC | 2 cut(s) | 907, 2274 |
| BspT107I | GGYRCC | 1 cut(s) | 2584 |
| BsrDI | GCAATG | 1 cut(s) | 1892 |
| BsrFI | RCCGGY | 1 cut(s) | 2632 |
| BsrI | ACTGG | 5 cut(s) | 243, 388, 537, 1977, 2139 |
| BssAI | RCCGGY | 1 cut(s) | 2632 |
| BssECI | CCNNGG | 4 cut(s) | 14, 475, 2354, 2580 |
| BssMI | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| BssNI | GRCGYC | 2 cut(s) | 65, 244 |
| BssT1I | CCWWGG | 2 cut(s) | 475, 2354 |
| Bst2UI | CCWGG | 2 cut(s) | 635, 1343 |
| Bst4CI | ACNGT | 8 cut(s) | 555, 904, 1240, 1392, 1565, 1816, 2113, 2159 |
| Bst6I | CTCTTC | 3 cut(s) | 907, 1911, 2274 |
| BstACI | GRCGYC | 2 cut(s) | 65, 244 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 1511, 2342, 2655 |
| BstC8I | GCNNGC | 7 cut(s) | 60, 146, 768, 1080, 1404, 2200, 2660 |
| BstDEI | CTNAG | 5 cut(s) | 512, 1614, 2000, 2366, 2464 |
| BstDSI | CCRYGG | 2 cut(s) | 475, 2354 |
| BstF5I | GGATG | 7 cut(s) | 10, 657, 973, 1682, 1826, 2329, 2338 |
| BstHHI | GCGC | 1 cut(s) | 2664 |
| BstKTI | GATC | 8 cut(s) | 417, 679, 1597, 1716, 2015, 2351, 2611, 2646 |
| BstMAI | GTCTC | 4 cut(s) | 38, 2125, 2229, 2465 |
| BstMBI | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 326, 1343, 1511, 1534, 2273, 2333, 2342, 2655 |
| BstNI | CCWGG | 2 cut(s) | 635, 1343 |
| BstNSI | RCATGY | 5 cut(s) | 148, 1044, 1058, 1645, 2655 |
| BstSCI | CCNGG | 3 cut(s) | 13, 633, 1341 |
| BstSFI | CTRYAG | 2 cut(s) | 903, 2235 |
| BstSLI | GKGCMC | 2 cut(s) | 934, 1709 |
| BstV1I | GCAGC | 4 cut(s) | 304, 1934, 1990, 2590 |
| BstV2I | GAAGAC | 2 cut(s) | 693, 2051 |
| BstX2I | RGATCY | 3 cut(s) | 1713, 2012, 2348 |
| BstYI | RGATCY | 3 cut(s) | 1713, 2012, 2348 |
| Bsu15I | ATCGAT | 1 cut(s) | 129 |
| BsuI | GTATCC | 1 cut(s) | 1513 |
| BsuRI | GGCC | 1 cut(s) | 1681 |
| BsuTUI | ATCGAT | 1 cut(s) | 129 |
| BtgI | CCRYGG | 2 cut(s) | 475, 2354 |
| BtsCI | GGATG | 7 cut(s) | 10, 657, 973, 1682, 1826, 2329, 2338 |
| BtsI | GCAGTG | 3 cut(s) | 265, 417, 1523 |
| BtsIMutI | CAGTG | 4 cut(s) | 265, 417, 1236, 1523 |
| BveI | ACCTGC | 1 cut(s) | 1139 |
| Cac8I | GCNNGC | 7 cut(s) | 60, 146, 768, 1080, 1404, 2200, 2660 |
| CaiI | CAGNNNCTG | 1 cut(s) | 836 |
| CciI | TCATGA | 2 cut(s) | 117, 445 |
| CfoI | GCGC | 1 cut(s) | 2664 |
| Cfr10I | RCCGGY | 1 cut(s) | 2632 |
| Cfr13I | GGNCC | 1 cut(s) | 2635 |
| ClaI | ATCGAT | 1 cut(s) | 129 |
| CseI | GACGC | 2 cut(s) | 54, 252 |
| Csp6I | GTAC | 1 cut(s) | 536 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1192, 1227 |
| CviQI | GTAC | 1 cut(s) | 536 |
| DdeI | CTNAG | 5 cut(s) | 512, 1614, 2000, 2366, 2464 |
| DpnI | GATC | 8 cut(s) | 416, 678, 1596, 1715, 2014, 2350, 2610, 2645 |
| DpnII | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| DraI | TTTAAA | 3 cut(s) | 1254, 1672, 2164 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 1011, 1580 |
| DriI | GACNNNNNGTC | 1 cut(s) | 501 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 1011, 1580 |
| EaeI | YGGCCR | 1 cut(s) | 1679 |
| Eam1104I | CTCTTC | 3 cut(s) | 907, 1911, 2274 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 501 |
| EarI | CTCTTC | 3 cut(s) | 907, 1911, 2274 |
| Eco130I | CCWWGG | 2 cut(s) | 475, 2354 |
| Eco47I | GGWCC | 1 cut(s) | 2635 |
| Eco57I | CTGAAG | 5 cut(s) | 369, 756, 858, 1822, 2234 |
| Eco88I | CYCGRG | 1 cut(s) | 1775 |
| EcoRI | GAATTC | 1 cut(s) | 2446 |
| EcoRII | CCWGG | 2 cut(s) | 633, 1341 |
| EcoT14I | CCWWGG | 2 cut(s) | 475, 2354 |
| EcoT22I | ATGCAT | 4 cut(s) | 1516, 1593, 2338, 2672 |
| ErhI | CCWWGG | 2 cut(s) | 475, 2354 |
| Esp3I | CGTCTC | 1 cut(s) | 38 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1679, 1711 |
| FaqI | GGGAC | 2 cut(s) | 255, 1017 |
| FauI | CCCGC | 1 cut(s) | 400 |
| FauNDI | CATATG | 1 cut(s) | 2666 |
| FblI | GTMKAC | 1 cut(s) | 235 |
| Fnu4HI | GCNGC | 5 cut(s) | 63, 318, 1948, 1979, 2604 |
| FokI | GGATG | 6 cut(s) | 664, 980, 1689, 1813, 2336, 2345 |
| Fsp4HI | GCNGC | 5 cut(s) | 63, 318, 1948, 1979, 2604 |
| FspBI | CTAG | 4 cut(s) | 1403, 1710, 1986, 2639 |
| FspI | TGCGCA | 1 cut(s) | 2663 |
| GlaI | GCGC | 1 cut(s) | 2663 |
| GluI | GCNGC | 5 cut(s) | 63, 318, 1948, 1979, 2604 |
| GsuI | CTGGAG | 3 cut(s) | 1680, 2472, 2516 |
| HaeIII | GGCC | 1 cut(s) | 1681 |
| HapII | CCGG | 3 cut(s) | 14, 1407, 2633 |
| HgaI | GACGC | 2 cut(s) | 54, 252 |
| HhaI | GCGC | 1 cut(s) | 2664 |
| Hin1I | GRCGYC | 2 cut(s) | 65, 244 |
| Hin6I | GCGC | 1 cut(s) | 2662 |
| HinP1I | GCGC | 1 cut(s) | 2662 |
| HincII | GTYRAC | 2 cut(s) | 1573, 2569 |
| HindII | GTYRAC | 2 cut(s) | 1573, 2569 |
| HindIII | AAGCTT | 5 cut(s) | 768, 1382, 1535, 1922, 2402 |
| HpaI | GTTAAC | 1 cut(s) | 2569 |
| HpaII | CCGG | 3 cut(s) | 14, 1407, 2633 |
| Hpy166II | GTNNAC | 6 cut(s) | 236, 760, 1479, 1573, 1707, 2569 |
| Hpy188I | TCNGA | 7 cut(s) | 600, 694, 702, 1176, 1586, 2209, 2256 |
| Hpy8I | GTNNAC | 6 cut(s) | 236, 760, 1479, 1573, 1707, 2569 |
| HpyAV | CCTTC | 7 cut(s) | 81, 101, 290, 535, 658, 902, 2174 |
| HpyCH4III | ACNGT | 8 cut(s) | 555, 904, 1240, 1392, 1565, 1816, 2113, 2159 |
| HpyCH4IV | ACGT | 3 cut(s) | 232, 1124, 1931 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 326, 1343, 1511, 1534, 2273, 2333, 2342, 2655 |
| HpyF3I | CTNAG | 5 cut(s) | 512, 1614, 2000, 2366, 2464 |
| HpySE526I | ACGT | 3 cut(s) | 232, 1124, 1931 |
| Hsp92I | GRCGYC | 2 cut(s) | 65, 244 |
| HspAI | GCGC | 1 cut(s) | 2662 |
| KspAI | GTTAAC | 1 cut(s) | 2569 |
| Kzo9I | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| LguI | GCTCTTC | 2 cut(s) | 907, 2274 |
| LmnI | GCTCC | 1 cut(s) | 2541 |
| Lsp1109I | GCAGC | 4 cut(s) | 304, 1934, 1990, 2590 |
| LweI | GCATC | 7 cut(s) | 19, 642, 1065, 1276, 2254, 2314, 2323 |
| MaeI | CTAG | 4 cut(s) | 1403, 1710, 1986, 2639 |
| MaeII | ACGT | 3 cut(s) | 232, 1124, 1931 |
| MaeIII | GTNAC | 5 cut(s) | 66, 824, 1036, 1151, 1234 |
| MalI | GATC | 8 cut(s) | 416, 678, 1596, 1715, 2014, 2350, 2610, 2645 |
| MboI | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| MfeI | CAATTG | 2 cut(s) | 657, 2048 |
| MflI | RGATCY | 3 cut(s) | 1713, 2012, 2348 |
| MhlI | GDGCHC | 2 cut(s) | 934, 1709 |
| MlsI | TGGCCA | 1 cut(s) | 1681 |
| MluNI | TGGCCA | 1 cut(s) | 1681 |
| MlyI | GAGTC | 3 cut(s) | 23, 683, 928 |
| MmeI | TCCRAC | 2 cut(s) | 1539, 1854 |
| Mox20I | TGGCCA | 1 cut(s) | 1681 |
| Mph1103I | ATGCAT | 4 cut(s) | 1516, 1593, 2338, 2672 |
| MroXI | GAANNNNTTC | 4 cut(s) | 372, 470, 843, 1734 |
| MscI | TGGCCA | 1 cut(s) | 1681 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1434 |
| Msp20I | TGGCCA | 1 cut(s) | 1681 |
| MspI | CCGG | 3 cut(s) | 14, 1407, 2633 |
| MspR9I | CCNGG | 3 cut(s) | 15, 635, 1343 |
| MunI | CAATTG | 2 cut(s) | 657, 2048 |
| Mva1269I | GAATGC | 4 cut(s) | 1516, 1593, 1769, 2506 |
| MvaI | CCWGG | 2 cut(s) | 635, 1343 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 326, 1343, 1511, 1534, 2273, 2333, 2342, 2655 |
| NciI | CCSGG | 1 cut(s) | 15 |
| NcoI | CCATGG | 2 cut(s) | 475, 2354 |
| NdeI | CATATG | 1 cut(s) | 2666 |
| NdeII | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| NheI | GCTAGC | 1 cut(s) | 1402 |
| NlaIV | GGNNCC | 1 cut(s) | 2586 |
| NmeAIII | GCCGAG | 2 cut(s) | 2206, 2561 |
| NmuCI | GTSAC | 4 cut(s) | 66, 1036, 1151, 1234 |
| NsbI | TGCGCA | 1 cut(s) | 2663 |
| NsiI | ATGCAT | 4 cut(s) | 1516, 1593, 2338, 2672 |
| NspI | RCATGY | 5 cut(s) | 148, 1044, 1058, 1645, 2655 |
| PaeI | GCATGC | 1 cut(s) | 148 |
| PaeR7I | CTCGAG | 1 cut(s) | 1775 |
| PagI | TCATGA | 2 cut(s) | 117, 445 |
| PaqCI | CACCTGC | 1 cut(s) | 1139 |
| PciI | ACATGT | 3 cut(s) | 1054, 1641, 2651 |
| PciSI | GCTCTTC | 2 cut(s) | 907, 2274 |
| PctI | GAATGC | 4 cut(s) | 1516, 1593, 1769, 2506 |
| PdmI | GAANNNNTTC | 4 cut(s) | 372, 470, 843, 1734 |
| PflFI | GACNNNGTC | 1 cut(s) | 1580 |
| PkrI | GCNGC | 5 cut(s) | 64, 319, 1949, 1980, 2605 |
| PleI | GAGTC | 3 cut(s) | 23, 683, 927 |
| PpsI | GAGTC | 3 cut(s) | 23, 683, 927 |
| PscI | ACATGT | 3 cut(s) | 1054, 1641, 2651 |
| PshBI | ATTAAT | 2 cut(s) | 882, 1229 |
| PsiI | TTATAA | 1 cut(s) | 1362 |
| Psp1406I | AACGTT | 1 cut(s) | 1931 |
| Psp6I | CCWGG | 2 cut(s) | 633, 1341 |
| PspGI | CCWGG | 2 cut(s) | 633, 1341 |
| PspN4I | GGNNCC | 1 cut(s) | 2586 |
| PspPI | GGNCC | 1 cut(s) | 2635 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 213, 245 |
| PstI | CTGCAG | 1 cut(s) | 2239 |
| PstNI | CAGNNNCTG | 1 cut(s) | 836 |
| PsuI | RGATCY | 3 cut(s) | 1713, 2012, 2348 |
| PsyI | GACNNNGTC | 1 cut(s) | 1580 |
| RsaI | GTAC | 1 cut(s) | 537 |
| RsaNI | GTAC | 1 cut(s) | 536 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1434 |
| SapI | GCTCTTC | 2 cut(s) | 907, 2274 |
| SatI | GCNGC | 5 cut(s) | 63, 318, 1948, 1979, 2604 |
| Sau3AI | GATC | 8 cut(s) | 414, 676, 1594, 1713, 2012, 2348, 2608, 2643 |
| Sau96I | GGNCC | 1 cut(s) | 2635 |
| SchI | GAGTC | 3 cut(s) | 23, 683, 928 |
| ScrFI | CCNGG | 3 cut(s) | 15, 635, 1343 |
| SduI | GDGCHC | 2 cut(s) | 934, 1709 |
| SfaNI | GCATC | 7 cut(s) | 19, 642, 1065, 1276, 2254, 2314, 2323 |
| SfcI | CTRYAG | 2 cut(s) | 903, 2235 |
| Sfr274I | CTCGAG | 1 cut(s) | 1775 |
| SinI | GGWCC | 1 cut(s) | 2635 |
| SlaI | CTCGAG | 1 cut(s) | 1775 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1434 |
| SmlI | CTYRAG | 3 cut(s) | 297, 1775, 2399 |
| SmoI | CTYRAG | 3 cut(s) | 297, 1775, 2399 |
| SphI | GCATGC | 1 cut(s) | 148 |
| SsiI | CCGC | 4 cut(s) | 62, 407, 588, 1618 |
| SspI | AATATT | 2 cut(s) | 1735, 2311 |
| SspMI | CTAG | 4 cut(s) | 1403, 1710, 1986, 2639 |
| StyD4I | CCNGG | 3 cut(s) | 13, 633, 1341 |
| StyI | CCWWGG | 2 cut(s) | 475, 2354 |
| TaaI | ACNGT | 8 cut(s) | 555, 904, 1240, 1392, 1565, 1816, 2113, 2159 |
| TaiI | ACGT | 3 cut(s) | 235, 1127, 1934 |
| TaqI | TCGA | 4 cut(s) | 129, 1051, 1101, 1776 |
| TauI | GCSGC | 1 cut(s) | 65 |
| TscAI | CASTG | 4 cut(s) | 272, 424, 1243, 1530 |
| TseFI | GTSAC | 4 cut(s) | 66, 1036, 1151, 1234 |
| TseI | GCWGC | 4 cut(s) | 317, 1947, 1978, 2603 |
| Tsp45I | GTSAC | 4 cut(s) | 66, 1036, 1151, 1234 |
| TspGWI | ACGGA | 2 cut(s) | 62, 195 |
| TspRI | CASTG | 4 cut(s) | 272, 424, 1243, 1530 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1580 |
| VneI | GTGCAC | 1 cut(s) | 1705 |
| VpaK11BI | GGWCC | 1 cut(s) | 2635 |
| VspI | ATTAAT | 2 cut(s) | 882, 1229 |
| XapI | RAATTY | 7 cut(s) | 372, 569, 855, 1029, 1091, 1995, 2446 |
| XceI | RCATGY | 5 cut(s) | 148, 1044, 1058, 1645, 2655 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1756 |
| XhoI | CTCGAG | 1 cut(s) | 1775 |
| XmiI | GTMKAC | 1 cut(s) | 235 |
| XmnI | GAANNNNTTC | 4 cut(s) | 372, 470, 843, 1734 |
| XspI | CTAG | 4 cut(s) | 1403, 1710, 1986, 2639 |
| Zsp2I | ATGCAT | 4 cut(s) | 1516, 1593, 2338, 2672 |