MD09G1274400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000042
Physical Location & Seq
Reverse (-)
35126636 .. 35134346
7711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1274400.v1.1.491

Sequence Viewer

Length: 4398 bp
ATGAAGCATGAAGTTGATGAAGTCGGAAGAAAAGTTGATCAAAAAGTGGAAGCTGATGTCCAGAAATGGCAGTCAGATGCGGAGAAGACCACTCTGGAGGCAAAAGCACTCTTGCATGATGAAGGCGCAAAGACAAAGTGTTTCATTATATGTCCTAATCTGATATCCTATCATCAACGTAGCAGGAAGGCAACAAAACTGATGAAAAAGATTGAAGAACATGAAAATAAAAAAAAAGAATTCTCCAGTGTTTCTTACAAAGCTCCCGTAGAAGACATATCTGCCATAGCCTCCGACGAGTACATGGCCTTTGAATCCAGGATTTCTATGGTGAAGGATATCGTTACAGAACTGAAAAAACCTGATATCAACAGGATTGGGGTGTACGGATTAGGGGGCGTTGGGAAGACAACACTTGCCAAAGAGGTTTACAGAGAAGTTGTGAAAGAGAAGTTATTTGATGATGTGGTTATAATACTAAATGTGAAAGAAAAAAAAGACAATGAAAAAATTCAAAATGAAATTACCAGAAAGTTGAGCATGGATGTTGATGAATCAAAGGACATGGGAACAAGGGCGAGTCTCCTACGGGCCAGGATAAAAGATGGGAAGACTCTTGTCATTTTAGATGATGTTTTGGAAAGAATTGACTTTGAGGCTGTGGGACTTGTCGGTGTGCCGAACTGTAAGTTATTGTTGACATCTAGAGAAAAAAAAGTTTTCTTCTCTGATATGCGTACCCAAAAAGAATTTCCACTTGGATTTTTAAGCGAAAAAGAAAGTTGGAGTTTGTTTGAGAAGATGGCAGGTGATGTTGTTAAAGACAACCGTATACTGAAGGAAGCAACCCAGCTAGCAAAGAAATGTGGAGGTTTGCCGGTTTTGGTTGTTGCAGTTGCAAGTGCTTTAAGGGATGGTAGTTTGGAGGAATGGAAAGATGCGTTGAGAAGTTTCAAAAGATTTGACAAGAAAGAGTTGAATGAAAAAGCATTCTTGGCTCTAAAGTGGAGTTACGAACAATTGGAGGATCAAGAGCTTAAGCAATTGTTCTTGCTTTGTGGGAGTAGCTGCAATTATCTCAGTGACTTGTTGAAGTATAGTATGGGTTTGGGTTTGATAAAAAATGTTGAGACAGTGGAAGAGGCACGGACTTCATTGAATGTAATGGTTAAAAAATTAAAAAATTCTTGCCTATTGCAGGACAGCTATGACGATGACACTGTTAGAATGCATGAACTTGTACGAGATGTTGCTGTCCGGATTGCAACTGTTGATCAAAAAGCCTTTTCAAGAGCATATGGAGATGAGGTGAAAGAATGGCCAACTGAGGATTTCCTTGAAAAATGCACAAGGATGTCCTTAAAACGTTGTAAGATCCCCAGGCTTCCTGAAGTACCTTGGGAATGCCCAGAATTAAAATTGTTGATCCTGGATATTGGTAGTATTGGCAACTCCCACGAAATCCCAAGCAAATTTTTTGAAGGGATGAAAGAACTCAAAGTGCTGGATGTAACCAGATTCAGTATTCAGTCACTACCTCCATCTCTTCAATCCCTAAAGCATCTCCACACATTGTGCTTAGATCAATGCGAGTTGGTAGACATAACTCTTGTTGGACAGCTAACAAACTTGAAAATTCTTAGCCTCCTAAAGTCCAAGATTAAAAAGTTGCCCAAAGAAATAGGGCAATTGACAAGGCTACAATTATTGGATTTGACCGGTTGCTCTGAACTTGTTCTGATCTTACCTGGTGTTATATCAAGCTTGACAAGACTAGAAGACTTGAGGATGGGAATAAAAAGCTTTAAGCAATGGGAGGGTGAAGGTCCCACTAGCGGAGGAAGTAATGCTACTGTTTCAGAGCTGAAGCACTTGGCTGAACTAACTGCATTAGACATACATGTTCCAGATGCTGACCTTCTTCCACCGAACTTGTTCTCCGATAAGTTGGAAAGATACAACATACTTATCGGTGATTGTTGGGAGTATCCTGACACGTACGGAACCTCCTCTAACATGCTAAAACTGAAGCTCACCAGGAGAAATCAATTCGACCGAGGTATAAAGTTGCTGGTAAAGAGATGTGAGCAATTGTACTTGGATGGGAAGGAGAGTGGGAATATTATTTCCTTTCTATTTGACAGTGATGCTTGTCTCCATGTCCAAAACAACGACGAAGTTACATATGTCATTAACTTCGTCAGTTGGAGTTATTCTCATAATACCTTCCCCAACCTGGAATCTCTAACTCTTGAAGACCTTGTGACTTTGGAAAGTGTATGTTATGGCCAACTTGTAGGCGAGCCCTTTCAAAAGTTAAAATCTTTGACACTTCGGAATCTACCGAAGCTTATTGGTTTCTTTTCCAAAGACAAGCGAACGATTGGTACAGATGCCGACGAAATCGTTTTGGAGGGAGAAGTTGGTGGACCACCGAGACTTTTCAGTAATGGAGAGGTTAAGATGCCCAACTTAACAACCTTGATTGTGCATCAATGTGATAGTTTAAGATTTTTGTTTTCGAGTTCCATGGCTAAATGTCTTGGACAACTCAAAAATCTCAAAATATCTAATTGTCAAATCATGGAAGAGATAGTGGGAAATGAGGAGAAAACAGATGACATGTTTGATAAGCTAAGCCATCTAGAGTTACAACATCTTCCAAACCTTGCTAGATTCAGCTCAGGAAGTTACATTAAATTTCCGTCTTTGGCGTATCTGAATCTAGACAATTGTACTAAACTGCAGACTTTCATCTTTGATGCTAAGAGTGAAAATATTACAACCAACAAAGAAGAAAGAGATATTGAGCTCTTTGATGAAAAGGTAGGATTTCCTAGCTTGGTGGTATTGTGGATACAGGACTTACCTAAATTGAAGACAATATTCCACAACCAACTTCATTCAGATTCTTTTGGCAAGCTCAGAATTATGGATGTTCGTAGATGCCACAGTCTAATTAATATATTTGGGCCAAGTATCATGGGTAGATTGAATGCTCTAGAGTTATTACGAATAATGCATTGCCAGTCACTACAGGTGGTATATGACACATCTATATTCAGTTTGAACCGTTTTGAGTGCCCAAATCTATACTCAGTTTGGATAGACTCATGTGAGAGTTTGAAAAATGTATTTCCCACCTCAGTGACCAAAGATCTTAAGCAGCTAAACAATTTCAGTGTCAAGAATTGTGGTTTAATGGAGGAAATTGTTGTGAAGGAAGAAGGACCACAAACGACATACGAGGAGTTCGAGTTCCCTAAAGTAACAAATATGGAATTTGATAATCTACCCCAACTTCGGAGTTTCTATCCAGGACTGCATGTTTCTAATTGGCCATCACTCAACATATTAAAGTTCAAGAAATGTGGTGGTGTGAAAATTTTTGCTGTTGAATTTTCAACATATCAAGACAAGTTTAAGTTGGGCCACCCAATACCAAAGGAACAACCTTTCTTTTTAATTGAGAAGGGTAAGTCATTCCTCAACTTGGAAGTGTTGATGTTGGACCGGAACACGGAGATTTGGTATGAACGGTATGGTCCACTCCCGACAGAGTTGTTGAGTAAAGTAAAGACAATTCTTTTTGCTACTTCACATCCCAAGTCAGATGTTTTCTTTGAGAATTTACAGAATACTGAAATGCTTGTTGTGCTCTCTGCTCCTTGGAAAGAATTATTTGTCCACGACCACCAAGGAAGTAGTAGAGGGGAAACACATGAAGTTGAGATCCTCCCACGTGTAAAGGTTTTGCAGCTCACCAAAATGCCGGAGCTGATTCATCTAGGAATGGAAAACTCCCAACCAGGGGGCCCTGTTCTTCCAAACTTGGAAGTTCTAAGGCTGATCAAATGTGGTAGATTAGAGAATCTAGCTTCAACTTTGATATCCTTTCGGAATCTAACCTTTTTGACCATACGGAGTTGTCACGGACTGCAATACTTAAAACCTTATTCTGTGGCTAAGAATTTGCAGCAACTCATGGGGTTGGAAGTTGTGTCCTGTCAAAGAATGGTAGAAATAGTAGCGACCAACGAAGATGATCCAGAAAATGAGATTACTTTCAGTTGCTTGCAACATTTGAAGCTTTCTGGTCTACCAAGTTTGCAAGGCTTTTGCAGGGGAAATTGCATTTTCAAAGTCCCATCCTTAAAAACTTTGATTGTCGAGGACTGCCAGCTGATCGACTTAAAGATTTCTTCTGATGGGTTACTCCAAAGTGATCCAAGACCAGAAAGATTACAAATGGCAGAGGAAATTGATGATGTACCGATGCTAAGTGATTCAGAGGAGAATGATCATGATCAAACTGAGCACCTGATTATTGCAGATTCAAATGTAGATGTTACAATTGCAGGACTCTCTGCCAAAAAGGAATCCTCATCTTTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1466

Amino Acids

166.48

Weight (kDa)

5.82

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 110 - 270 2.6e-26 NB-ARC domain
LRR_14 PF23598 503 - 636 2.4e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 818 - 860 3e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 862 - 1010 7.6e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1012 - 1066 3.5e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1297 - 1394 5.4e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AarI CACCTGC 1 cut(s) 797
Acc36I ACCTGC 1 cut(s) 797
AccI GTMKAC 3 cut(s) 832, 1599, 4099
AccIII TCCGGA 1 cut(s) 1257
AciI CCGC 2 cut(s) 80, 1836
AclI AACGTT 1 cut(s) 1366
AclWI GGATC 6 cut(s) 1035, 1369, 1420, 3727, 4040, 4220
AcoI YGGCCR 3 cut(s) 1319, 2287, 3338
AcuI CTGAAG 4 cut(s) 857, 1410, 1886, 2048
AcvI CACGTG 1 cut(s) 3743
AdeI CACNNNGTG 1 cut(s) 1575
AfiI CCNNNNNNNGG 8 cut(s) 1198, 1835, 2236, 3303, 3493, 3520, 3810, 3811
AflII CTTAAG 2 cut(s) 1037, 3161
AflIII ACRYGT 4 cut(s) 1900, 1995, 2622, 3742
AgeI ACCGGT 1 cut(s) 1718
AjnI CCWGG 9 cut(s) 317, 593, 1379, 1428, 1747, 2036, 2235, 3316, 3808
AjuI GAANNNNNNNTTGG 4 cut(s) 735, 741, 767, 773
AleI CACNNNNGTG 1 cut(s) 3146
AloI GAACNNNNNNTCC 4 cut(s) 1922, 1954, 3242, 3274
Alw21I GWGCWC 3 cut(s) 2814, 3660, 4320
Alw26I GTCTC 4 cut(s) 587, 1124, 2159, 2431
AlwI GGATC 6 cut(s) 1035, 1369, 1420, 3727, 4040, 4220
AlwNI CAGNNNCTG 1 cut(s) 1913
Aor13HI TCCGGA 1 cut(s) 1257
AoxI GGCC 8 cut(s) 306, 591, 1319, 2287, 2972, 3338, 3430, 3814
ApaI GGGCCC 1 cut(s) 3818
ApeKI GCWGC 4 cut(s) 1068, 3166, 3757, 3976
AseI ATTAAT 1 cut(s) 2961
AsiGI ACCGGT 1 cut(s) 1718
Asp700I GAANNNNTTC 3 cut(s) 510, 1734, 1934
AspLEI GCGC 1 cut(s) 128
AsuHPI GGTGA 7 cut(s) 343, 821, 1321, 1832, 1985, 2026, 3754
AsuNHI GCTAGC 1 cut(s) 853
AvaII GGWCC 5 cut(s) 1826, 2429, 3230, 3511, 3545
BaeGI GKGCMC 2 cut(s) 3086, 3818
BalI TGGCCA 3 cut(s) 1321, 2289, 3340
BanII GRGCYC 3 cut(s) 2307, 2814, 3818
BarI GAAGNNNNNNTAC 2 cut(s) 1834, 1866
BbrPI CACGTG 1 cut(s) 3743
BbsI GAAGAC 7 cut(s) 92, 279, 413, 617, 1785, 2262, 2885
Bbv12I GWGCWC 3 cut(s) 2814, 3660, 4320
BbvI GCAGC 4 cut(s) 1055, 3178, 3769, 3988
BciT130I CCWGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
BciVI GTATCC 2 cut(s) 1998, 2850
BclI TGATCA 5 cut(s) 37, 1273, 3849, 4300, 4306
BcoDI GTCTC 4 cut(s) 587, 1124, 2159, 2431
BfmI CTRYAG 2 cut(s) 2744, 3035
BfrI CTTAAG 2 cut(s) 1037, 3161
BfuAI ACCTGC 1 cut(s) 797
BfuI GTATCC 2 cut(s) 1998, 2850
BglII AGATCT 1 cut(s) 3157
BisI GCNGC 4 cut(s) 1069, 3167, 3758, 3977
BlpI GCTNAGC 1 cut(s) 2636
BlsI GCNGC 4 cut(s) 1070, 3168, 3759, 3978
Bme1390I CCNGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
Bme18I GGWCC 5 cut(s) 1826, 2429, 3230, 3511, 3545
BmiI GGNNCC 4 cut(s) 1828, 2005, 3815, 3816
BmrFI CCNGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
BmtI GCTAGC 1 cut(s) 857
BoxI GACNNNNGTC 1 cut(s) 617
BpiI GAAGAC 7 cut(s) 92, 279, 413, 617, 1785, 2262, 2885
BplI GAGNNNNNCTC 2 cut(s) 2200, 2232
BpmI CTGGAG 2 cut(s) 116, 229
Bpu10I CCTNAGC 1 cut(s) 2683
Bpu1102I GCTNAGC 1 cut(s) 2636
BpuEI CTTGAG 1 cut(s) 1804
BsaAI YACGTR 2 cut(s) 1998, 3743
BsaBI GATNNNNATC 1 cut(s) 4305
BsaJI CCNNGG 7 cut(s) 1379, 1397, 2056, 2529, 3668, 3697, 3809
BsaWI WCCGGW 3 cut(s) 1257, 1718, 3513
Bsc4I CCNNNNNNNGG 8 cut(s) 1198, 1835, 2236, 3303, 3493, 3520, 3810, 3811
Bse118I RCCGGY 2 cut(s) 877, 1718
Bse1I ACTGG 2 cut(s) 246, 3028
Bse3DI GCAATG 2 cut(s) 1817, 3022
Bse8I GATNNNNATC 1 cut(s) 4305
BseAI TCCGGA 1 cut(s) 1257
BseBI CCWGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
BseDI CCNNGG 7 cut(s) 1379, 1397, 2056, 2529, 3668, 3697, 3809
BseJI GATNNNNATC 1 cut(s) 4305
BseLI CCNNNNNNNGG 8 cut(s) 1198, 1835, 2236, 3303, 3493, 3520, 3810, 3811
BseMI GCAATG 2 cut(s) 1817, 3022
BseMII CTCAG 7 cut(s) 1093, 1317, 2697, 2938, 3111, 3159, 4305
BseNI ACTGG 2 cut(s) 246, 3028
BseRI GAGGAG 4 cut(s) 1999, 2621, 3263, 4307
BseSI GKGCMC 2 cut(s) 3086, 3818
BseXI GCAGC 4 cut(s) 1055, 3178, 3769, 3988
BseYI CCCAGC 1 cut(s) 849
Bsh1285I CGRYCG 1 cut(s) 2056
BshFI GGCC 8 cut(s) 308, 593, 1321, 2289, 2974, 3340, 3432, 3816
BshTI ACCGGT 1 cut(s) 1718
BsiEI CGRYCG 1 cut(s) 2056
BsiHKAI GWGCWC 3 cut(s) 2814, 3660, 4320
BsiSI CCGG 5 cut(s) 878, 1258, 1719, 3514, 3773
BsiWI CGTACG 1 cut(s) 1998
BslFI GGGAC 3 cut(s) 678, 1812, 4130
BslI CCNNNNNNNGG 8 cut(s) 1198, 1835, 2236, 3303, 3493, 3520, 3810, 3811
BsmAI GTCTC 4 cut(s) 587, 1124, 2159, 2431
BsmFI GGGAC 3 cut(s) 678, 1812, 4130
BsmI GAATGC 4 cut(s) 989, 1233, 1409, 3001
BsnI GGCC 8 cut(s) 308, 593, 1321, 2289, 2974, 3340, 3432, 3816
Bsp120I GGGCCC 1 cut(s) 3814
Bsp1286I GDGCHC 6 cut(s) 2307, 2814, 3086, 3660, 3818, 4320
Bsp13I TCCGGA 1 cut(s) 1257
Bsp1720I GCTNAGC 1 cut(s) 2636
Bsp19I CCATGG 1 cut(s) 2529
BspACI CCGC 2 cut(s) 80, 1836
BspANI GGCC 8 cut(s) 308, 593, 1321, 2289, 2974, 3340, 3432, 3816
BspCNI CTCAG 7 cut(s) 1092, 1318, 2696, 2937, 3110, 3158, 4306
BspEI TCCGGA 1 cut(s) 1257
BspHI TCATGA 1 cut(s) 4303
BspLI GGNNCC 4 cut(s) 1828, 2005, 3815, 3816
BspMAI CTGCAG 1 cut(s) 2748
BspMI ACCTGC 1 cut(s) 797
BspOI GCTAGC 1 cut(s) 857
BspPI GGATC 6 cut(s) 1035, 1369, 1420, 3727, 4040, 4220
BspTI CTTAAG 2 cut(s) 1037, 3161
BsrDI GCAATG 2 cut(s) 1817, 3022
BsrFI RCCGGY 2 cut(s) 877, 1718
BsrI ACTGG 2 cut(s) 246, 3028
BssAI RCCGGY 2 cut(s) 877, 1718
BssECI CCNNGG 7 cut(s) 1379, 1397, 2056, 2529, 3668, 3697, 3809
BssNAI GTATAC 1 cut(s) 833
BssT1I CCWWGG 4 cut(s) 1397, 2529, 3668, 3697
Bst1107I GTATAC 1 cut(s) 833
Bst2UI CCWGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
Bst6I CTCTTC 3 cut(s) 1134, 1551, 2583
BstAFI CTTAAG 2 cut(s) 1037, 3161
BstBAI YACGTR 2 cut(s) 1998, 3743
BstC8I GCNNGC 5 cut(s) 855, 2303, 2921, 4076, 4181
BstDSI CCRYGG 1 cut(s) 2529
BstENI CCTNNNNNAGG 1 cut(s) 1196
BstHHI GCGC 1 cut(s) 128
BstMAI GTCTC 4 cut(s) 587, 1124, 2159, 2431
BstMCI CGRYCG 1 cut(s) 2056
BstMWI GCNNNNNNNGC 2 cut(s) 995, 3655
BstNI CCWGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
BstNSI RCATGY 4 cut(s) 1904, 2020, 2626, 3329
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 9 cut(s) 317, 593, 1379, 1428, 1747, 2036, 2235, 3316, 3808
BstSFI CTRYAG 2 cut(s) 2744, 3035
BstSLI GKGCMC 2 cut(s) 3086, 3818
BstV1I GCAGC 4 cut(s) 1055, 3178, 3769, 3988
BstV2I GAAGAC 7 cut(s) 92, 279, 413, 617, 1785, 2262, 2885
BstX2I RGATCY 3 cut(s) 1374, 3157, 3732
BstYI RGATCY 3 cut(s) 1374, 3157, 3732
BstZ17I GTATAC 1 cut(s) 833
BsuI GTATCC 2 cut(s) 1998, 2850
BsuRI GGCC 8 cut(s) 308, 593, 1321, 2289, 2974, 3340, 3432, 3816
BtgI CCRYGG 1 cut(s) 2529
BtsIMutI CAGTG 7 cut(s) 253, 1087, 1140, 1218, 2149, 3153, 3187
BveI ACCTGC 1 cut(s) 797
Cac8I GCNNGC 5 cut(s) 855, 2303, 2921, 4076, 4181
CaiI CAGNNNCTG 1 cut(s) 1913
CciI TCATGA 1 cut(s) 4303
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 2 cut(s) 877, 1718
CsiI ACCWGGT 1 cut(s) 1747
CspAI ACCGGT 1 cut(s) 1718
CspCI CAANNNNNGTGG 6 cut(s) 1914, 1949, 3175, 3210, 3352, 3387
DraIII CACNNNGTG 1 cut(s) 1575
EaeI YGGCCR 3 cut(s) 1319, 2287, 3338
Eam1104I CTCTTC 3 cut(s) 1134, 1551, 2583
EarI CTCTTC 3 cut(s) 1134, 1551, 2583
Ecl136II GAGCTC 1 cut(s) 2812
Eco130I CCWWGG 4 cut(s) 1397, 2529, 3668, 3697
Eco24I GRGCYC 3 cut(s) 2307, 2814, 3818
Eco32I GATATC 4 cut(s) 165, 340, 367, 3891
Eco47I GGWCC 5 cut(s) 1826, 2429, 3230, 3511, 3545
Eco53kI GAGCTC 1 cut(s) 2812
Eco57I CTGAAG 4 cut(s) 857, 1410, 1886, 2048
Eco72I CACGTG 1 cut(s) 3743
EcoICRI GAGCTC 1 cut(s) 2812
EcoNI CCTNNNNNAGG 1 cut(s) 1196
EcoO109I RGGNCCY 3 cut(s) 1826, 3814, 3815
EcoRI GAATTC 1 cut(s) 239
EcoRII CCWGG 9 cut(s) 317, 593, 1379, 1428, 1747, 2036, 2235, 3316, 3808
EcoRV GATATC 4 cut(s) 165, 340, 367, 3891
EcoT14I CCWWGG 4 cut(s) 1397, 2529, 3668, 3697
EcoT22I ATGCAT 2 cut(s) 1233, 3024
EcoT38I GRGCYC 3 cut(s) 2307, 2814, 3818
ErhI CCWWGG 4 cut(s) 1397, 2529, 3668, 3697
FaqI GGGAC 3 cut(s) 678, 1812, 4130
FauNDI CATATG 2 cut(s) 1297, 2185
FbaI TGATCA 5 cut(s) 37, 1273, 3849, 4300, 4306
FblI GTMKAC 3 cut(s) 832, 1599, 4099
Fnu4HI GCNGC 4 cut(s) 1069, 3167, 3758, 3977
FriOI GRGCYC 3 cut(s) 2307, 2814, 3818
Fsp4HI GCNGC 4 cut(s) 1069, 3167, 3758, 3977
GlaI GCGC 1 cut(s) 127
GluI GCNGC 4 cut(s) 1069, 3167, 3758, 3977
GsaI CCCAGC 1 cut(s) 853
GsuI CTGGAG 2 cut(s) 116, 229
HaeIII GGCC 8 cut(s) 308, 593, 1321, 2289, 2974, 3340, 3432, 3816
HapII CCGG 5 cut(s) 878, 1258, 1719, 3514, 3773
HhaI GCGC 1 cut(s) 128
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 699
HindII GTYRAC 1 cut(s) 699
HindIII AAGCTT 4 cut(s) 1762, 1801, 2348, 4088
HpaII CCGG 5 cut(s) 878, 1258, 1719, 3514, 3773
HphI GGTGA 7 cut(s) 343, 821, 1321, 1832, 1985, 2026, 3754
Hpy166II GTNNAC 9 cut(s) 385, 430, 699, 833, 1600, 2429, 3548, 3688, 4100
Hpy8I GTNNAC 9 cut(s) 385, 430, 699, 833, 1600, 2429, 3548, 3688, 4100
Hpy99I CGWCG 3 cut(s) 299, 2177, 2402
HpyCH4IV ACGT 4 cut(s) 178, 1366, 1997, 3742
HpyF10VI GCNNNNNNNGC 2 cut(s) 995, 3655
HpySE526I ACGT 4 cut(s) 178, 1366, 1997, 3742
HspAI GCGC 1 cut(s) 126
Kpn2I TCCGGA 1 cut(s) 1257
Ksp22I TGATCA 5 cut(s) 37, 1273, 3849, 4300, 4306
LmnI GCTCC 3 cut(s) 268, 3670, 3775
Lsp1109I GCAGC 4 cut(s) 1055, 3178, 3769, 3988
MabI ACCWGGT 1 cut(s) 1747
MaeII ACGT 4 cut(s) 178, 1366, 1997, 3742
MfeI CAATTG 6 cut(s) 1019, 1043, 1688, 2090, 2731, 4353
MflI RGATCY 3 cut(s) 1374, 3157, 3732
MhlI GDGCHC 6 cut(s) 2307, 2814, 3086, 3660, 3818, 4320
MlsI TGGCCA 3 cut(s) 1321, 2289, 3340
MluNI TGGCCA 3 cut(s) 1321, 2289, 3340
MlyI GAGTC 4 cut(s) 589, 607, 3104, 4356
MmeI TCCRAC 8 cut(s) 4, 318, 764, 1594, 1929, 2186, 3489, 3972
Mox20I TGGCCA 3 cut(s) 1321, 2289, 3340
Mph1103I ATGCAT 2 cut(s) 1233, 3024
MroI TCCGGA 1 cut(s) 1257
MroXI GAANNNNTTC 3 cut(s) 510, 1734, 1934
MscI TGGCCA 3 cut(s) 1321, 2289, 3340
MslI CAYNNNNRTG 4 cut(s) 1352, 2496, 3146, 3767
Msp20I TGGCCA 3 cut(s) 1321, 2289, 3340
MspA1I CMGCKG 1 cut(s) 4183
MspCI CTTAAG 2 cut(s) 1037, 3161
MspI CCGG 5 cut(s) 878, 1258, 1719, 3514, 3773
MspR9I CCNGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
MunI CAATTG 6 cut(s) 1019, 1043, 1688, 2090, 2731, 4353
Mva1269I GAATGC 4 cut(s) 989, 1233, 1409, 3001
MvaI CCWGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
MwoI GCNNNNNNNGC 2 cut(s) 995, 3655
NcoI CCATGG 1 cut(s) 2529
NdeI CATATG 2 cut(s) 1297, 2185
NheI GCTAGC 1 cut(s) 853
NlaIV GGNNCC 4 cut(s) 1828, 2005, 3815, 3816
NmuCI GTSAC 6 cut(s) 1082, 1530, 2263, 3030, 3148, 3929
NsiI ATGCAT 2 cut(s) 1233, 3024
NspI RCATGY 4 cut(s) 1904, 2020, 2626, 3329
OliI CACNNNNGTG 1 cut(s) 3146
PagI TCATGA 1 cut(s) 4303
PaqCI CACCTGC 1 cut(s) 797
PciI ACATGT 2 cut(s) 1900, 2622
PcsI WCGNNNNNNNCGW 1 cut(s) 3252
PctI GAATGC 4 cut(s) 989, 1233, 1409, 3001
PdmI GAANNNNTTC 3 cut(s) 510, 1734, 1934
Pfl23II CGTACG 1 cut(s) 1998
PfoI TCCNGGA 3 cut(s) 317, 1428, 3316
PinAI ACCGGT 1 cut(s) 1718
PkrI GCNGC 4 cut(s) 1070, 3168, 3759, 3978
PleI GAGTC 4 cut(s) 588, 607, 3104, 4356
PmaCI CACGTG 1 cut(s) 3743
PmlI CACGTG 1 cut(s) 3743
PpsI GAGTC 4 cut(s) 588, 607, 3104, 4356
Ppu21I YACGTR 2 cut(s) 1998, 3743
PpuMI RGGWCCY 1 cut(s) 1826
PscI ACATGT 2 cut(s) 1900, 2622
PshAI GACNNNNGTC 1 cut(s) 617
PshBI ATTAAT 1 cut(s) 2961
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 1 cut(s) 2814
Psp1406I AACGTT 1 cut(s) 1366
Psp5II RGGWCCY 1 cut(s) 1826
Psp6I CCWGG 9 cut(s) 317, 593, 1379, 1428, 1747, 2036, 2235, 3316, 3808
PspCI CACGTG 1 cut(s) 3743
PspFI CCCAGC 1 cut(s) 849
PspGI CCWGG 9 cut(s) 317, 593, 1379, 1428, 1747, 2036, 2235, 3316, 3808
PspLI CGTACG 1 cut(s) 1998
PspN4I GGNNCC 4 cut(s) 1828, 2005, 3815, 3816
PspOMI GGGCCC 1 cut(s) 3814
PspPPI RGGWCCY 1 cut(s) 1826
PsrI GAACNNNNNNTAC 2 cut(s) 2371, 2403
PstI CTGCAG 1 cut(s) 2748
PstNI CAGNNNCTG 1 cut(s) 1913
PsuI RGATCY 3 cut(s) 1374, 3157, 3732
PvuII CAGCTG 1 cut(s) 4183
RseI CAYNNNNRTG 4 cut(s) 1352, 2496, 3146, 3767
SacI GAGCTC 1 cut(s) 2814
SatI GCNGC 4 cut(s) 1069, 3167, 3758, 3977
SchI GAGTC 4 cut(s) 589, 607, 3104, 4356
ScrFI CCNGG 9 cut(s) 319, 595, 1381, 1430, 1749, 2038, 2237, 3318, 3810
SduI GDGCHC 6 cut(s) 2307, 2814, 3086, 3660, 3818, 4320
SexAI ACCWGGT 1 cut(s) 1747
SfcI CTRYAG 2 cut(s) 2744, 3035
SinI GGWCC 5 cut(s) 1826, 2429, 3230, 3511, 3545
SmiMI CAYNNNNRTG 4 cut(s) 1352, 2496, 3146, 3767
SmlI CTYRAG 3 cut(s) 1037, 1783, 3161
SmoI CTYRAG 3 cut(s) 1037, 1783, 3161
SsiI CCGC 2 cut(s) 80, 1836
SspI AATATT 3 cut(s) 2122, 2779, 2886
SstI GAGCTC 1 cut(s) 2814
StyD4I CCNGG 9 cut(s) 317, 593, 1379, 1428, 1747, 2036, 2235, 3316, 3808
StyI CCWWGG 4 cut(s) 1397, 2529, 3668, 3697
TaiI ACGT 4 cut(s) 181, 1369, 2000, 3745
TaqI TCGA 5 cut(s) 2052, 2522, 3255, 4170, 4188
TaqII GACCGA 1 cut(s) 2070
TatI WGTACW 3 cut(s) 300, 2094, 2735
TscAI CASTG 7 cut(s) 253, 1087, 1140, 1225, 2149, 3153, 3187
TseFI GTSAC 6 cut(s) 1082, 1530, 2263, 3030, 3148, 3929
TseI GCWGC 4 cut(s) 1068, 3166, 3757, 3976
Tsp45I GTSAC 6 cut(s) 1082, 1530, 2263, 3030, 3148, 3929
TspGWI ACGGA 7 cut(s) 402, 1162, 2016, 2694, 3536, 3937, 3948
TspRI CASTG 7 cut(s) 253, 1087, 1140, 1225, 2149, 3153, 3187
Vha464I CTTAAG 2 cut(s) 1037, 3161
VpaK11BI GGWCC 5 cut(s) 1826, 2429, 3230, 3511, 3545
VspI ATTAAT 1 cut(s) 2961
XagI CCTNNNNNAGG 1 cut(s) 1196
XbaI TCTAGA 4 cut(s) 704, 2644, 2725, 3001
XceI RCATGY 4 cut(s) 1904, 2020, 2626, 3329
XcmI CCANNNNNNNNNTGG 1 cut(s) 325
XmiI GTMKAC 3 cut(s) 832, 1599, 4099
XmnI GAANNNNTTC 3 cut(s) 510, 1734, 1934
Zsp2I ATGCAT 2 cut(s) 1233, 3024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.