pycom09g18360

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000042
Physical Location & Seq
Reverse (-)
19011200 .. 19014712
3513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g18360.3

Sequence Viewer

Length: 2418 bp
ATGATGATGAGACTGTTAGAATGCATGAGCTTGTACGAGATGTTGCTGTCCGGATTGCATCTGATCGATCAAAAGGCCTTTTCAAGAGCATATGGAGATGAGGTGAAAGAATGGCCAACTGAGGATTTCCTTAAAAAATGCACAAGGATGTCCTTAGATGGTTGCATGATCCCCAGGCTTCCTGAAGTACCTTGGGAATGCCCAGAACTAAAATTGTTAATCTTGTCCGATAATTATATGGGCGACTCCCAGGAAATCCCAAGCAAATTTTTTGAAGGGATGAAAGAACTCAAAGTGTTGGATGTCAGCGGAATTTGTATTCCATCACTACCTCCGTCTCTTCTATCCCTAAAGCATCTCCAGACATTGTGTTTAGATCGGTGCGAGTTGGTAGACATAACTCTCGTTGGACAGCTAACAAACTTGAAAATTCTTAGCTTCCTAGGGTCCAAGATTAATGAGTTACCCAAAGAAATTGGGCAATTGACTAGCCTACGTCTATTGGATTTGACTGGTTGCTCTGAACTTGTTCTGATCTCACCTGGTGTTATATCAAGCTTGACAAGACTAGAAGACTTGAGGATGGGAATAAAAAGCTTCAAGCAATGGGAGGGTGAAGGTTTGGTCGATGGCAGAAGTAATGCTAGCGTTTCAGAGCTGAAGCATTTGTCTCATTTGTCCGCATTAGACATACATGTTCTAGATGCTAACCTTCTTCCAACTAACTTGTTCTCCGATAAGTTAGAAAGATACACTATACTTATCGGTGATTGTTGGGAATATCCTGACATCTATGGAACCTCCTCGAACATGCTAAAACTCAAGCTTACAAGGAGAAATCAGTTCGACCGAGGTATAAAGTTGTTGGTAAAGAAATGTGAGCAATTGTACTTGGATGGGATGGAGATTATGAATATTATTTCCTATCTATTTGATAGTGAAGCTATTAATCAACTGAAGCATCTCCACGTCCAAAACAACGACGAAGTTACATATGTCATTAAATCTACTAGGTGGAGTTATTCTCATAATGCCTTCCCCAGCCTGGAATCTCTCTCTCTTGAAAACCTTGTGAGTTTGGAAAGTGTATGTTATGGGCAACTCATAGGCGAGCCTTACCATAAGTTAAAATCTTTGACACTACGGAATCTACCAAAGCTTATTGGTTTCTCTTCCAAAAGCCACCAATTAACGTTTGCTACAAAAGCCGACGAAATCGTTACGGAAGATGAAGCTGGTGGACCACCGAGACTTTTCAATAATGAGGAGGTTTCGATGCCCAACTTAACAACCTTGATTGTGCATCAATGTGATAGTTTAAGATTCTTGTTTTCGAGTTCCATGGCTAAATCTCTCAGGCAACTAAAACATCTCAAGATATCCAAATGTCAGGCACTACAAGCAGTATATGACACATCATCTACCACTCAATTGAACGATTTTGAGTGCCCAAATCTAAATTCAGTTGAGATAGACTTGTGTGACAGTTTGAAAAATGTCTTTCCCTCTTCAATGGCCAAAGAGCTTAAGCAGCTAAGCAAGTTGAAGGTTGAGAATTGTGGTTTAATGGAGGAAATTGTTGCGAATGAAGAAGGACCACAAGTGACGTACAAGGAGTTCAGGTTCCCTAAAGTAACCTATATGATATTTAATAATCTACCTCAACTTCGGAGATTTTATCCATCACTGCATGTTTCTAATTGGCCATCACTCAACGTATTAAATTTCACGAAATGTGGTGGTGTGGGGATTTTTGCTGCTGAATTTTCAACATATCATGACAAACTTGAGTTGGGTCACTCAACACCAATGAAACAGCCTTTCTTTTTAATTGAGAAGGGCAAGTCATTTCTCAACTTGGAATACTTGACGTTGGACGAGAACACGGAGATTTGGTATGAACCGTATGGTCCACTCCAGGCAGAGTTGTTGAGCAGACTCAAATCAATTGAGTTTGCTACTTCACATCCCAAGTCAGATGTTTTCTTCGAGAATTTACAGAACATTGAAGATCTCCGTGTGCTCTTTGCTCCTTGGAAAGAATTATCTGTCCACCACCACCAAGGAAGTAGCAGAGGGGAAATACATGAAGTAGGGACCCTCTCATACGTAAAGATTTTGTGGCTCATCAATATGCCAGAGCTGATACATCTAGGAAAGGAAAACTTCCAACCAGGGGACCTTGTTTTTCCAAACTTGGAAGTTCTATGCGTGCACAAATGTGGCAGATTAGAGAATCTAGCCTCAACTTTGATATCCTTTCAGAATCTAACCATTTTGAATATATGGAGTTGTCACGTAATGCAATACTTAATACCTTATTATGTGGCTAAGAATTTGCAGCAACTCAAGAAGTTGGTAGTTGAGTACTGTCAAAGAATGGTAGAAATCGTAGCGAGCAACAGAGATGATCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

806

Amino Acids

91.94

Weight (kDa)

5.8

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 393
AccIII TCCGGA 1 cut(s) 50
AciI CCGC 2 cut(s) 309, 681
AclI AACGTT 1 cut(s) 1193
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 3 cut(s) 113, 1515, 1703
AcsI RAATTY 8 cut(s) 266, 312, 429, 1459, 1723, 1763, 1993, 2335
AcuI CTGAAG 3 cut(s) 204, 680, 977
AdeI CACNNNGTG 1 cut(s) 545
AfaI GTAC 5 cut(s) 35, 189, 890, 1610, 2369
AfiI CCNNNNNNNGG 2 cut(s) 1045, 2176
AflII CTTAAG 1 cut(s) 1526
AflIII ACRYGT 1 cut(s) 694
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 6 cut(s) 173, 249, 541, 1044, 1917, 2173
AjuI GAANNNNNNNTTGG 2 cut(s) 1444, 1476
AleI CACNNNNGTG 1 cut(s) 2220
AloI GAACNNNNNNTCC 2 cut(s) 1607, 1639
Alw21I GWGCWC 2 cut(s) 2025, 2217
Alw26I GTCTC 4 cut(s) 4, 342, 675, 1243
Alw44I GTGCAC 1 cut(s) 2213
AlwI GGATC 1 cut(s) 163
Aor13HI TCCGGA 1 cut(s) 50
AoxI GGCC 4 cut(s) 75, 113, 1515, 1703
ApaLI GTGCAC 1 cut(s) 2213
ApeKI GCWGC 3 cut(s) 1531, 1757, 2341
ApoI RAATTY 8 cut(s) 266, 312, 429, 1459, 1723, 1763, 1993, 2335
AseI ATTAAT 2 cut(s) 456, 948
Asp700I GAANNNNTTC 1 cut(s) 528
AspA2I CCTAGG 1 cut(s) 442
AspS9I GGNCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
AsuHPI GGTGA 4 cut(s) 115, 531, 626, 779
AsuNHI GCTAGC 1 cut(s) 644
AvaII GGWCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
AvrII CCTAGG 1 cut(s) 442
BaeGI GKGCMC 2 cut(s) 1451, 2217
BalI TGGCCA 3 cut(s) 115, 1517, 1705
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 2 cut(s) 2025, 2217
BbvI GCAGC 3 cut(s) 1543, 1744, 2353
BccI CCATC 8 cut(s) 152, 331, 577, 623, 890, 895, 1690, 1714
BciT130I CCWGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
BcoDI GTCTC 4 cut(s) 4, 342, 675, 1243
BfaI CTAG 8 cut(s) 443, 489, 569, 645, 701, 1011, 2153, 2240
BfrI CTTAAG 1 cut(s) 1526
BglII AGATCT 1 cut(s) 2011
BisI GCNGC 3 cut(s) 1532, 1758, 2342
BlnI CCTAGG 1 cut(s) 442
BlpI GCTNAGC 1 cut(s) 1535
BlsI GCNGC 3 cut(s) 1533, 1759, 2343
BmcAI AGTACT 1 cut(s) 2369
Bme1390I CCNGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
Bme18I GGWCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
BmiI GGNNCC 6 cut(s) 448, 799, 1625, 2098, 2099, 2180
BmrFI CCNGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
BmsI GCATC 6 cut(s) 67, 364, 694, 970, 1266, 1312
BmtI GCTAGC 1 cut(s) 648
BpiI GAAGAC 1 cut(s) 579
BplI GAGNNNNNCTC 2 cut(s) 1009, 1041
BpmI CTGGAG 2 cut(s) 344, 1901
Bpu1102I GCTNAGC 1 cut(s) 1535
BpuEI CTTGAG 5 cut(s) 598, 806, 1358, 1808, 2333
Bsa29I ATCGAT 2 cut(s) 66, 2413
BsaAI YACGTR 2 cut(s) 2110, 2299
BsaJI CCNNGG 9 cut(s) 173, 191, 249, 442, 850, 1341, 2033, 2062, 2174
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 8 cut(s) 87, 117, 716, 746, 1607, 1637, 1880, 1910
Bsc4I CCNNNNNNNGG 2 cut(s) 1045, 2176
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 611
BseAI TCCGGA 1 cut(s) 50
BseBI CCWGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
BseCI ATCGAT 2 cut(s) 66, 2413
BseDI CCNNGG 9 cut(s) 173, 191, 249, 442, 850, 1341, 2033, 2062, 2174
BseGI GGATG 7 cut(s) 153, 285, 307, 588, 901, 906, 1966
BseLI CCNNNNNNNGG 2 cut(s) 1045, 2176
BseMI GCAATG 1 cut(s) 611
BseMII CTCAG 2 cut(s) 111, 1369
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 2 cut(s) 793, 1280
BseSI GKGCMC 2 cut(s) 1451, 2217
BseXI GCAGC 3 cut(s) 1543, 1744, 2353
BseYI CCCAGC 1 cut(s) 1040
Bsh1285I CGRYCG 1 cut(s) 850
BshFI GGCC 4 cut(s) 77, 115, 1517, 1705
BshVI ATCGAT 2 cut(s) 66, 2413
BsiEI CGRYCG 1 cut(s) 850
BsiHKAI GWGCWC 2 cut(s) 2025, 2217
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 2 cut(s) 2110, 2192
BslI CCNNNNNNNGG 2 cut(s) 1045, 2176
BsmAI GTCTC 4 cut(s) 4, 342, 675, 1243
BsmBI CGTCTC 1 cut(s) 342
BsmFI GGGAC 2 cut(s) 2110, 2192
BsmI GAATGC 2 cut(s) 26, 203
BsnI GGCC 4 cut(s) 77, 115, 1517, 1705
Bsp1286I GDGCHC 3 cut(s) 1451, 2025, 2217
Bsp13I TCCGGA 1 cut(s) 50
Bsp143I GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
Bsp1720I GCTNAGC 1 cut(s) 1535
Bsp19I CCATGG 1 cut(s) 1341
BspACI CCGC 2 cut(s) 309, 681
BspANI GGCC 4 cut(s) 77, 115, 1517, 1705
BspCNI CTCAG 2 cut(s) 112, 1368
BspDI ATCGAT 2 cut(s) 66, 2413
BspEI TCCGGA 1 cut(s) 50
BspHI TCATGA 1 cut(s) 1777
BspLI GGNNCC 6 cut(s) 448, 799, 1625, 2098, 2099, 2180
BspOI GCTAGC 1 cut(s) 648
BspPI GGATC 1 cut(s) 163
BspTI CTTAAG 1 cut(s) 1526
BsrDI GCAATG 1 cut(s) 611
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 9 cut(s) 173, 191, 249, 442, 850, 1341, 2033, 2062, 2174
BssMI GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
BssT1I CCWWGG 5 cut(s) 191, 442, 1341, 2033, 2062
Bst2UI CCWGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
Bst4CI ACNGT 4 cut(s) 15, 1487, 1905, 2372
Bst6I CTCTTC 3 cut(s) 345, 1177, 1513
BstAFI CTTAAG 1 cut(s) 1526
BstBAI YACGTR 2 cut(s) 2110, 2299
BstC8I GCNNGC 4 cut(s) 646, 1112, 2213, 2398
BstDEI CTNAG 6 cut(s) 120, 154, 434, 1355, 1535, 2331
BstDSI CCRYGG 1 cut(s) 1341
BstF5I GGATG 7 cut(s) 153, 285, 307, 588, 901, 906, 1966
BstKTI GATC 7 cut(s) 66, 70, 171, 379, 537, 2014, 2413
BstMAI GTCTC 4 cut(s) 4, 342, 675, 1243
BstMBI GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
BstMCI CGRYCG 1 cut(s) 850
BstMWI GCNNNNNNNGC 3 cut(s) 1205, 1400, 1531
BstNI CCWGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
BstNSI RCATGY 3 cut(s) 698, 814, 1694
BstSCI CCNGG 6 cut(s) 173, 249, 541, 1044, 1917, 2173
BstSLI GKGCMC 2 cut(s) 1451, 2217
BstSNI TACGTA 1 cut(s) 2110
BstV1I GCAGC 3 cut(s) 1543, 1744, 2353
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 1 cut(s) 2011
BstYI RGATCY 1 cut(s) 2011
Bsu15I ATCGAT 2 cut(s) 66, 2413
BsuRI GGCC 4 cut(s) 77, 115, 1517, 1705
BsuTUI ATCGAT 2 cut(s) 66, 2413
BtgI CCRYGG 1 cut(s) 1341
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 7 cut(s) 153, 285, 307, 588, 901, 906, 1966
BtsI GCAGTG 1 cut(s) 1685
BtsIMutI CAGTG 1 cut(s) 1685
Cac8I GCNNGC 4 cut(s) 646, 1112, 2213, 2398
CciI TCATGA 1 cut(s) 1777
Cfr13I GGNCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
ClaI ATCGAT 2 cut(s) 66, 2413
CsiI ACCWGGT 1 cut(s) 541
Csp6I GTAC 5 cut(s) 34, 188, 889, 1609, 2368
CviAII CATG 8 cut(s) 25, 166, 695, 811, 1342, 1691, 1778, 2087
CviQI GTAC 5 cut(s) 34, 188, 889, 1609, 2368
DdeI CTNAG 6 cut(s) 120, 154, 434, 1355, 1535, 2331
DpnI GATC 7 cut(s) 65, 69, 170, 378, 536, 2013, 2412
DpnII GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
DraIII CACNNNGTG 1 cut(s) 545
EaeI YGGCCR 3 cut(s) 113, 1515, 1703
Eam1104I CTCTTC 3 cut(s) 345, 1177, 1513
EarI CTCTTC 3 cut(s) 345, 1177, 1513
Eco105I TACGTA 1 cut(s) 2110
Eco130I CCWWGG 5 cut(s) 191, 442, 1341, 2033, 2062
Eco147I AGGCCT 1 cut(s) 77
Eco32I GATATC 2 cut(s) 1380, 2256
Eco47I GGWCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
Eco57I CTGAAG 3 cut(s) 204, 680, 977
EcoO109I RGGNCCY 2 cut(s) 2097, 2179
EcoRII CCWGG 6 cut(s) 173, 249, 541, 1044, 1917, 2173
EcoRV GATATC 2 cut(s) 1380, 2256
EcoT14I CCWWGG 5 cut(s) 191, 442, 1341, 2033, 2062
EcoT22I ATGCAT 1 cut(s) 26
ErhI CCWWGG 5 cut(s) 191, 442, 1341, 2033, 2062
Esp3I CGTCTC 1 cut(s) 342
FaeI CATG 8 cut(s) 28, 169, 698, 814, 1345, 1694, 1781, 2090
FalI AAGNNNNNCTT 2 cut(s) 2150, 2182
FaqI GGGAC 2 cut(s) 2110, 2192
FatI CATG 8 cut(s) 24, 165, 694, 810, 1341, 1690, 1777, 2086
FauNDI CATATG 2 cut(s) 91, 994
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 1532, 1758, 2342
FokI GGATG 7 cut(s) 160, 292, 314, 595, 908, 913, 1953
Fsp4HI GCNGC 3 cut(s) 1532, 1758, 2342
FspBI CTAG 8 cut(s) 443, 489, 569, 645, 701, 1011, 2153, 2240
GluI GCNGC 3 cut(s) 1532, 1758, 2342
GsaI CCCAGC 1 cut(s) 1044
GsuI CTGGAG 2 cut(s) 344, 1901
HaeIII GGCC 4 cut(s) 77, 115, 1517, 1705
HapII CCGG 1 cut(s) 51
Hin1II CATG 8 cut(s) 28, 169, 698, 814, 1345, 1694, 1781, 2090
HindIII AAGCTT 4 cut(s) 556, 595, 824, 1157
HinfI GANTC 7 cut(s) 245, 1049, 1147, 1323, 1938, 2236, 2266
HpaII CCGG 1 cut(s) 51
HphI GGTGA 4 cut(s) 115, 531, 626, 779
Hpy166II GTNNAC 5 cut(s) 394, 1241, 1913, 2053, 2215
Hpy188I TCNGA 9 cut(s) 63, 229, 523, 534, 655, 736, 1671, 1978, 2265
Hpy8I GTNNAC 5 cut(s) 394, 1241, 1913, 2053, 2215
Hpy99I CGWCG 2 cut(s) 986, 1214
HpyAV CCTTC 7 cut(s) 269, 611, 722, 1045, 1540, 1586, 1831
HpyCH4III ACNGT 4 cut(s) 15, 1487, 1905, 2372
HpyCH4IV ACGT 8 cut(s) 496, 969, 1193, 1607, 1716, 1871, 2109, 2298
HpyCH4V TGCA 9 cut(s) 24, 58, 141, 165, 1303, 1690, 2215, 2305, 2341
HpyF10VI GCNNNNNNNGC 3 cut(s) 1205, 1400, 1531
HpyF3I CTNAG 6 cut(s) 120, 154, 434, 1355, 1535, 2331
HpySE526I ACGT 8 cut(s) 496, 969, 1193, 1607, 1716, 1871, 2109, 2298
Hsp92II CATG 8 cut(s) 28, 169, 698, 814, 1345, 1694, 1781, 2090
KflI GGGWCCC 1 cut(s) 2097
Kpn2I TCCGGA 1 cut(s) 50
Kzo9I GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
LmnI GCTCC 1 cut(s) 2035
Lsp1109I GCAGC 3 cut(s) 1543, 1744, 2353
LweI GCATC 6 cut(s) 67, 364, 694, 970, 1266, 1312
MabI ACCWGGT 1 cut(s) 541
MaeI CTAG 8 cut(s) 443, 489, 569, 645, 701, 1011, 2153, 2240
MaeII ACGT 8 cut(s) 496, 969, 1193, 1607, 1716, 1871, 2109, 2298
MaeIII GTNAC 8 cut(s) 462, 988, 1219, 1481, 1603, 1633, 1796, 2294
MalI GATC 7 cut(s) 65, 69, 170, 378, 536, 2013, 2412
MboI GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
MboII GAAGA 9 cut(s) 332, 584, 707, 1164, 1238, 1500, 1601, 1978, 2021
MfeI CAATTG 4 cut(s) 482, 884, 1430, 1947
MflI RGATCY 1 cut(s) 2011
MhlI GDGCHC 3 cut(s) 1451, 2025, 2217
MlsI TGGCCA 3 cut(s) 115, 1517, 1705
MluNI TGGCCA 3 cut(s) 115, 1517, 1705
MlyI GAGTC 2 cut(s) 239, 1932
MmeI TCCRAC 5 cut(s) 279, 388, 743, 1854, 2194
Mox20I TGGCCA 3 cut(s) 115, 1517, 1705
Mph1103I ATGCAT 1 cut(s) 26
MroI TCCGGA 1 cut(s) 50
MroXI GAANNNNTTC 1 cut(s) 528
MscI TGGCCA 3 cut(s) 115, 1517, 1705
MslI CAYNNNNRTG 4 cut(s) 146, 1308, 2132, 2220
Msp20I TGGCCA 3 cut(s) 115, 1517, 1705
MspA1I CMGCKG 1 cut(s) 309
MspCI CTTAAG 1 cut(s) 1526
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
MunI CAATTG 4 cut(s) 482, 884, 1430, 1947
Mva1269I GAATGC 2 cut(s) 26, 203
MvaI CCWGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
MwoI GCNNNNNNNGC 3 cut(s) 1205, 1400, 1531
NcoI CCATGG 1 cut(s) 1341
NdeI CATATG 2 cut(s) 91, 994
NdeII GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
NheI GCTAGC 1 cut(s) 644
NlaIII CATG 8 cut(s) 28, 169, 698, 814, 1345, 1694, 1781, 2090
NlaIV GGNNCC 6 cut(s) 448, 799, 1625, 2098, 2099, 2180
NmuCI GTSAC 4 cut(s) 1481, 1603, 1796, 2294
NsiI ATGCAT 1 cut(s) 26
NspI RCATGY 3 cut(s) 698, 814, 1694
OliI CACNNNNGTG 1 cut(s) 2220
PagI TCATGA 1 cut(s) 1777
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 694
PctI GAATGC 2 cut(s) 26, 203
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 5 cut(s) 1049, 1147, 1323, 2236, 2266
PkrI GCNGC 3 cut(s) 1533, 1759, 2343
PleI GAGTC 2 cut(s) 239, 1932
PpsI GAGTC 2 cut(s) 239, 1932
Ppu21I YACGTR 2 cut(s) 2110, 2299
PpuMI RGGWCCY 2 cut(s) 2097, 2179
PscI ACATGT 1 cut(s) 694
PshBI ATTAAT 2 cut(s) 456, 948
Psp1406I AACGTT 1 cut(s) 1193
Psp5II RGGWCCY 2 cut(s) 2097, 2179
Psp6I CCWGG 6 cut(s) 173, 249, 541, 1044, 1917, 2173
PspFI CCCAGC 1 cut(s) 1040
PspGI CCWGG 6 cut(s) 173, 249, 541, 1044, 1917, 2173
PspN4I GGNNCC 6 cut(s) 448, 799, 1625, 2098, 2099, 2180
PspPI GGNCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
PspPPI RGGWCCY 2 cut(s) 2097, 2179
PsrI GAACNNNNNNTAC 2 cut(s) 1601, 1633
PsuI RGATCY 1 cut(s) 2011
RsaI GTAC 5 cut(s) 35, 189, 890, 1610, 2369
RsaNI GTAC 5 cut(s) 34, 188, 889, 1609, 2368
RseI CAYNNNNRTG 4 cut(s) 146, 1308, 2132, 2220
SatI GCNGC 3 cut(s) 1532, 1758, 2342
Sau3AI GATC 7 cut(s) 63, 67, 168, 376, 534, 2011, 2410
Sau96I GGNCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
ScaI AGTACT 1 cut(s) 2369
SchI GAGTC 2 cut(s) 239, 1932
ScrFI CCNGG 6 cut(s) 175, 251, 543, 1046, 1919, 2175
SduI GDGCHC 3 cut(s) 1451, 2025, 2217
SexAI ACCWGGT 1 cut(s) 541
SfaNI GCATC 6 cut(s) 67, 364, 694, 970, 1266, 1312
SinI GGWCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
SmiMI CAYNNNNRTG 4 cut(s) 146, 1308, 2132, 2220
SmlI CTYRAG 6 cut(s) 577, 821, 1373, 1526, 1787, 2348
SmoI CTYRAG 6 cut(s) 577, 821, 1373, 1526, 1787, 2348
SnaBI TACGTA 1 cut(s) 2110
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 2 cut(s) 309, 681
SspI AATATT 1 cut(s) 916
SspMI CTAG 8 cut(s) 443, 489, 569, 645, 701, 1011, 2153, 2240
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 6 cut(s) 173, 249, 541, 1044, 1917, 2173
StyI CCWWGG 5 cut(s) 191, 442, 1341, 2033, 2062
TaaI ACNGT 4 cut(s) 15, 1487, 1905, 2372
TaiI ACGT 8 cut(s) 499, 972, 1196, 1610, 1719, 1874, 2112, 2301
TaqI TCGA 8 cut(s) 66, 627, 806, 846, 1274, 1334, 1989, 2413
TaqII GACCGA 1 cut(s) 864
TatI WGTACW 2 cut(s) 888, 2367
TfiI GAWTC 5 cut(s) 1049, 1147, 1323, 2236, 2266
TscAI CASTG 1 cut(s) 1692
TseFI GTSAC 4 cut(s) 1481, 1603, 1796, 2294
TseI GCWGC 3 cut(s) 1531, 1757, 2341
Tsp45I GTSAC 4 cut(s) 1481, 1603, 1796, 2294
TspDTI ATGAA 7 cut(s) 296, 926, 1245, 1602, 1826, 1914, 2103
TspGWI ACGGA 5 cut(s) 324, 1159, 1238, 1901, 2006
TspRI CASTG 1 cut(s) 1692
Vha464I CTTAAG 1 cut(s) 1526
VneI GTGCAC 1 cut(s) 2213
VpaK11BI GGWCC 6 cut(s) 447, 1241, 1595, 1910, 2097, 2179
VspI ATTAAT 2 cut(s) 456, 948
XapI RAATTY 8 cut(s) 266, 312, 429, 1459, 1723, 1763, 1993, 2335
XbaI TCTAGA 1 cut(s) 700
XceI RCATGY 3 cut(s) 698, 814, 1694
XmaJI CCTAGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 8 cut(s) 443, 489, 569, 645, 701, 1011, 2153, 2240
ZrmI AGTACT 1 cut(s) 2369
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.