Rh1BG234600

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000042
Physical Location & Seq
Forward (+)
35777841 .. 35785811
7971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG234600.1

Sequence Viewer

Length: 2358 bp
ATGCTTAACTTCTCATCCGTGGGTCTCCGAGGAACCGTAGACATACAAGGAATGGATTGTCTTTGTGCAATTGGTAACGGGGTTGTTGGAAAAATAGCCGAGTACACGGTTGAACCCTTTGGACGTCTAGTGGGACGCCAAGTGGGTTATCTAATCCACTACAACAAGAATCTTCAAACTCTAATGACTCAAGTGAAGAATTTGAGCGTCGCCAGAGCTAGAGTAAAGCAACAGGTTGATGAAGCTGATAGAAGAGGTGAGAAAATCCATGAGGATGTCCAAAAATGGCTCGAAGAAGTGGCTCAGATCATGAAAGACACAGAAGAATTCTTGAAGGGAAAATGCCAAGCAACTGGAGAGCCAAACGAAGTCTTGAAGGATGAAGGCCAAGCAAATGAAGAGCCAAACGAAGTCTTGAAAGATGAACGTCAAGGAAAGCTGAAGCGTCTCCATCAATTTTGTCCTAATCCGAGACTTCGTTATCAACTAAGCAGAAAGTCAACAAAGTTGGTGGGGGCTGTGGATGAACTCTATAAAAGAAGAGACTTCCCTATTGTTTCATACAGTGTTCCCGCACCAGAGGTTGATAAACTTAAGAATTCTTGTCTATTGCTAGATGGAGATGACAATACATGTGTCAGAATGCATGATCTTGTACATGATGTTGCTATCTGGATTGCGACCAGAGATCAACACGTCCAAGTGAAAGATGGAGTTGATTTGATAGAATGGCCAGATCAAGATTCGTTTAAAAGGTGCAGTAATATCTCCTGGTCCTGTTGCAATATCCCCAGTCTTCCTGAAATACCTTGGAAATGTCCTGAGCTGGAAATGTTTCGTTTGCATGATGGATGCACGTTGGAAGACATAGCTCTAGTTGGAGAGCTAGGCAACTTGGAAACTCTTAGCTTTGCTAACTCCAAGGTCAAAGAGTTGCCGAAAGAAATTGCGAAATTGACTCATCTGCGGTTGTTAGACTTGTCGGATTGTCCTGAGCTTGAAGTGATTTCGCCTAATGTCATATCAAGTTTGACAAGGCTCGAAGACTTGAGAATGAAAAACAGTTTTGAAAAATGGGAGGCTGAGGAAGACATCAACAAAAGAAGGAATGCTAGTCTTTTAGAGCTGAAGAACTTATCGCAATTAACTGCATTAGAAATATATATTCCAGATGCTGATATTCTTCCACCAAACTTGTTCTCCAATAAATTAGAGAGATACCATATATATATTGGTGGTGTGTGGCCGAACCTTATGGCTGAAACGACCTTCAATACACTAAAGCTCGATCTTACTCCCACCAATCAATCGGATCAAGGTCTAAGAAGGCTGATGATGAAATCTGAAGATTTGGCCTTAAATATCATGGAAGGTGTTAATCATAGTTTGTGTCAGTTAGACAACGAAGGTTTTCTGCACCTGAAACGCCTCCTACTCCAAAACAATGTTGATCTTTCTTATATCAATGACAAGAAGGATAAACATGAGTCGGGCCCAGTAAAGTCTATCTTTCCAATTGACAAGGATTCATTCCCCAACTTGGAAATTCTGTCCGTGGACAACGTGGATATTTGGTACGGTACATCCCCAGTACCAACAGAGTCCTTCAGCAACCTAAAATGTGTTGCATTTAGTAATTGGGCACATTTCTCCCAGCAAGCTACGTCAGTCTTCCTTCAGAAATTACACAATATTGAAAGAATTGAAGTGATTAGGGCTCCTCCCGAAGAAATATTTGTCAGTGAAGGAAACAGTAGTTGTGAGGAAATACATGCAGTTGGGACCCTACCAAGCATACGAATTTTAAAGTTTGACGGGTGCAAAATGTTGCATCTAGGGAAGGAGAACTCCGGATCAGGAGGCCCAATTTTTCCAAACTTGGAAGTTCTAGAGGTGACGGAACATTCTCCCGGATTAAAGAATTTGGAATCATCAGCGATTTCCTTCCGGAATTTAACAACTTTGAAAGTAGTTGATTGTCCGGGCTTGGAACATTTAATAACTTATTCAGTGGCTAAAAGCTTAATGCAACTGACAACAATGGAAGTGAGTAGATGTCCAAGAATTGCAGTCATAGTGACGACAGACGGTGATGATGCAGCAGAAGAGATTACTTTCAGCCGATTGCAAGTTTTAACCCTTCGTGGACTGCCAAAAATGCAAGGCTTTTGCTCCGGAAGTTGCATTGTGAATCTCCCATCCTTGACTACTTTATCCATGAGCAACCGCCTTAAATTGAAGATTTTCCAAGAAAAGCTAAAGATAACAAGCAAGTACCCTGACACGGAGGAGGAGGAGGAAGACAATGTGGATGAGGAAATAGGCGTGCCGAGTGCTGAAACAAATATTGCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

785

Amino Acids

88.46

Weight (kDa)

5.2

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 293 - 392 2.2e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 642 - 743 4.3e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 127
AccI GTMKAC 1 cut(s) 39
AccIII TCCGGA 3 cut(s) 1850, 1947, 2174
AciI CCGC 3 cut(s) 573, 967, 2227
AclWI GGATC 2 cut(s) 1320, 1861
AcoI YGGCCR 2 cut(s) 731, 1244
AcsI RAATTY 7 cut(s) 199, 326, 598, 1545, 1800, 1921, 1951
AcuI CTGAAG 5 cut(s) 461, 1148, 1365, 1591, 1661
AcyI GRCGYC 2 cut(s) 124, 136
AfaI GTAC 6 cut(s) 104, 657, 1577, 1582, 1593, 2276
AfiI CCNNNNNNNGG 2 cut(s) 1540, 2284
AflII CTTAAG 1 cut(s) 593
AflIII ACRYGT 2 cut(s) 632, 694
AjiI CACGTC 1 cut(s) 697
AjnI CCWGG 1 cut(s) 770
Alw26I GTCTC 4 cut(s) 29, 452, 466, 537
AlwI GGATC 2 cut(s) 1320, 1861
AlwNI CAGNNNCTG 1 cut(s) 1175
Aor13HI TCCGGA 3 cut(s) 1850, 1947, 2174
AoxI GGCC 6 cut(s) 385, 731, 1244, 1353, 1492, 1861
ApaI GGGCCC 1 cut(s) 1496
ApeKI GCWGC 1 cut(s) 2099
ApoI RAATTY 7 cut(s) 199, 326, 598, 1545, 1800, 1921, 1951
Asp700I GAANNNNTTC 3 cut(s) 834, 1410, 2243
AspS9I GGNCC 5 cut(s) 774, 1492, 1493, 1782, 1862
AsuC2I CCSGG 2 cut(s) 1911, 1983
AsuHPI GGTGA 3 cut(s) 269, 1906, 2102
AvaII GGWCC 2 cut(s) 774, 1782
BaeGI GKGCMC 2 cut(s) 1496, 1645
BalI TGGCCA 1 cut(s) 733
BanII GRGCYC 2 cut(s) 1496, 1720
BbsI GAAGAC 6 cut(s) 788, 870, 1050, 1095, 1663, 2307
BbvCI CCTCAGC 1 cut(s) 1083
BbvI GCAGC 1 cut(s) 2111
BccI CCATC 5 cut(s) 459, 611, 704, 842, 2206
BciT130I CCWGG 1 cut(s) 772
BcnI CCSGG 2 cut(s) 1911, 1983
BcoDI GTCTC 4 cut(s) 29, 452, 466, 537
BfaI CTAG 9 cut(s) 128, 219, 614, 875, 887, 1113, 1835, 1889, 2356
BfrI CTTAAG 1 cut(s) 593
BisI GCNGC 1 cut(s) 2100
BlsI GCNGC 1 cut(s) 2101
Bme1390I CCNGG 3 cut(s) 772, 1911, 1983
Bme18I GGWCC 2 cut(s) 774, 1782
BmgBI CACGTC 1 cut(s) 697
BmgT120I GGNCC 5 cut(s) 774, 1492, 1493, 1782, 1862
BmiI GGNNCC 5 cut(s) 34, 1494, 1719, 1783, 1784
BmrFI CCNGG 3 cut(s) 772, 1911, 1983
BmrI ACTGGG 3 cut(s) 786, 1490, 1583
BmsI GCATC 4 cut(s) 842, 1162, 1840, 2086
BmuI ACTGGG 3 cut(s) 786, 1490, 1583
BpiI GAAGAC 6 cut(s) 788, 870, 1050, 1095, 1663, 2307
BpmI CTGGAG 1 cut(s) 375
Bpu10I CCTNAGC 3 cut(s) 822, 993, 1083
BpuEI CTTGAG 2 cut(s) 174, 1069
BpuMI CCSGG 2 cut(s) 1911, 1983
BsaHI GRCGYC 2 cut(s) 124, 136
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 5 cut(s) 18, 28, 809, 921, 1554
BsaWI WCCGGW 3 cut(s) 1850, 1947, 2174
BsaXI ACNNNNNCTCC 2 cut(s) 1184, 1214
Bsc4I CCNNNNNNNGG 2 cut(s) 1540, 2284
Bse1I ACTGG 4 cut(s) 358, 792, 1496, 1589
BseAI TCCGGA 3 cut(s) 1850, 1947, 2174
BseBI CCWGG 1 cut(s) 772
BseDI CCNNGG 5 cut(s) 18, 28, 809, 921, 1554
BseGI GGATG 8 cut(s) 14, 280, 385, 529, 857, 1583, 2198, 2317
BseLI CCNNNNNNNGG 2 cut(s) 1540, 2284
BseMII CTCAG 4 cut(s) 317, 813, 984, 1074
BseNI ACTGG 4 cut(s) 358, 792, 1496, 1589
BseRI GAGGAG 4 cut(s) 1710, 2303, 2306, 2309
BseSI GKGCMC 2 cut(s) 1496, 1645
BseXI GCAGC 1 cut(s) 2111
BseYI CCCAGC 1 cut(s) 1653
BsgI GTGCAG 2 cut(s) 778, 1400
BshFI GGCC 6 cut(s) 387, 733, 1246, 1355, 1494, 1863
BsiSI CCGG 5 cut(s) 1851, 1911, 1948, 1982, 2175
BslFI GGGAC 2 cut(s) 147, 1795
BslI CCNNNNNNNGG 2 cut(s) 1540, 2284
BsmAI GTCTC 4 cut(s) 29, 452, 466, 537
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 2 cut(s) 147, 1795
BsmI GAATGC 2 cut(s) 648, 1114
BsnI GGCC 6 cut(s) 387, 733, 1246, 1355, 1494, 1863
Bso31I GGTCTC 1 cut(s) 29
Bsp120I GGGCCC 1 cut(s) 1492
Bsp1286I GDGCHC 3 cut(s) 1496, 1645, 1720
Bsp13I TCCGGA 3 cut(s) 1850, 1947, 2174
Bsp1407I TGTACA 1 cut(s) 655
Bsp143I GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
BspACI CCGC 3 cut(s) 573, 967, 2227
BspANI GGCC 6 cut(s) 387, 733, 1246, 1355, 1494, 1863
BspCNI CTCAG 4 cut(s) 316, 814, 985, 1075
BspEI TCCGGA 3 cut(s) 1850, 1947, 2174
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 5 cut(s) 34, 1494, 1719, 1783, 1784
BspPI GGATC 2 cut(s) 1320, 1861
BspQI GCTCTTC 1 cut(s) 393
BspTI CTTAAG 1 cut(s) 593
BspTNI GGTCTC 1 cut(s) 29
BsrGI TGTACA 1 cut(s) 655
BsrI ACTGG 4 cut(s) 358, 792, 1496, 1589
BssECI CCNNGG 5 cut(s) 18, 28, 809, 921, 1554
BssMI GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
BssNI GRCGYC 2 cut(s) 124, 136
BssT1I CCWWGG 2 cut(s) 809, 921
Bst2UI CCWGG 1 cut(s) 772
Bst4CI ACNGT 7 cut(s) 37, 109, 566, 1064, 1580, 1754, 2090
Bst6I CTCTTC 4 cut(s) 247, 393, 535, 2100
BstACI GRCGYC 2 cut(s) 124, 136
BstAFI CTTAAG 1 cut(s) 593
BstAUI TGTACA 1 cut(s) 655
BstC8I GCNNGC 3 cut(s) 1659, 2327, 2353
BstDEI CTNAG 7 cut(s) 303, 488, 822, 905, 993, 1083, 1322
BstDSI CCRYGG 2 cut(s) 18, 1554
BstF5I GGATG 8 cut(s) 14, 280, 385, 529, 857, 1583, 2198, 2317
BstKTI GATC 8 cut(s) 309, 652, 691, 739, 1291, 1315, 1453, 1856
BstMAI GTCTC 4 cut(s) 29, 452, 466, 537
BstMBI GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
BstMWI GCNNNNNNNGC 1 cut(s) 2158
BstNI CCWGG 1 cut(s) 772
BstNSI RCATGY 2 cut(s) 636, 1775
BstSCI CCNGG 3 cut(s) 770, 1909, 1981
BstSLI GKGCMC 2 cut(s) 1496, 1645
BstV1I GCAGC 1 cut(s) 2111
BstV2I GAAGAC 6 cut(s) 788, 870, 1050, 1095, 1663, 2307
BstXI CCANNNNNNTGG 1 cut(s) 353
BsuRI GGCC 6 cut(s) 387, 733, 1246, 1355, 1494, 1863
BtgI CCRYGG 2 cut(s) 18, 1554
BtrI CACGTC 1 cut(s) 697
BtsCI GGATG 8 cut(s) 14, 280, 385, 529, 857, 1583, 2198, 2317
BtsIMutI CAGTG 3 cut(s) 571, 1747, 2016
Cac8I GCNNGC 3 cut(s) 1659, 2327, 2353
CaiI CAGNNNCTG 1 cut(s) 1175
CciI TCATGA 1 cut(s) 309
Cfr13I GGNCC 5 cut(s) 774, 1492, 1493, 1782, 1862
CseI GACGC 3 cut(s) 144, 196, 434
Csp6I GTAC 6 cut(s) 103, 656, 1576, 1581, 1592, 2275
CspCI CAANNNNNGTGG 4 cut(s) 492, 527, 1176, 1211
CviQI GTAC 6 cut(s) 103, 656, 1576, 1581, 1592, 2275
DdeI CTNAG 7 cut(s) 303, 488, 822, 905, 993, 1083, 1322
DpnI GATC 8 cut(s) 308, 651, 690, 738, 1290, 1314, 1452, 1855
DpnII GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
DraI TTTAAA 2 cut(s) 751, 1806
EaeI YGGCCR 2 cut(s) 731, 1244
Eam1104I CTCTTC 4 cut(s) 247, 393, 535, 2100
EarI CTCTTC 4 cut(s) 247, 393, 535, 2100
Eco130I CCWWGG 2 cut(s) 809, 921
Eco24I GRGCYC 2 cut(s) 1496, 1720
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 2 cut(s) 774, 1782
Eco57I CTGAAG 5 cut(s) 461, 1148, 1365, 1591, 1661
EcoO109I RGGNCCY 1 cut(s) 1782
EcoRI GAATTC 2 cut(s) 326, 598
EcoRII CCWGG 1 cut(s) 770
EcoT14I CCWWGG 2 cut(s) 809, 921
EcoT22I ATGCAT 1 cut(s) 648
EcoT38I GRGCYC 2 cut(s) 1496, 1720
ErhI CCWWGG 2 cut(s) 809, 921
Esp3I CGTCTC 1 cut(s) 452
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FaqI GGGAC 2 cut(s) 147, 1795
FauI CCCGC 1 cut(s) 580
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 1 cut(s) 2100
FokI GGATG 7 cut(s) 287, 392, 536, 864, 1570, 2185, 2324
FriOI GRGCYC 2 cut(s) 1496, 1720
Fsp4HI GCNGC 1 cut(s) 2100
FspBI CTAG 9 cut(s) 128, 219, 614, 875, 887, 1113, 1835, 1889, 2356
GluI GCNGC 1 cut(s) 2100
GsaI CCCAGC 1 cut(s) 1657
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 6 cut(s) 387, 733, 1246, 1355, 1494, 1863
HapII CCGG 5 cut(s) 1851, 1911, 1948, 1982, 2175
HgaI GACGC 3 cut(s) 144, 196, 434
Hin1I GRCGYC 2 cut(s) 124, 136
HincII GTYRAC 1 cut(s) 501
HindII GTYRAC 1 cut(s) 501
HindIII AAGCTT 1 cut(s) 2020
HinfI GANTC 9 cut(s) 169, 187, 743, 958, 1487, 1526, 1601, 1928, 2191
HpaII CCGG 5 cut(s) 1851, 1911, 1948, 1982, 2175
HphI GGTGA 3 cut(s) 269, 1906, 2102
Hpy166II GTNNAC 5 cut(s) 40, 105, 501, 1558, 2147
Hpy188I TCNGA 8 cut(s) 29, 306, 471, 641, 985, 1312, 1345, 1680
Hpy8I GTNNAC 5 cut(s) 40, 105, 501, 1558, 2147
Hpy99I CGWCG 1 cut(s) 212
HpyCH4III ACNGT 7 cut(s) 37, 109, 566, 1064, 1580, 1754, 2090
HpyCH4IV ACGT 6 cut(s) 124, 427, 696, 857, 1563, 1664
HpyF10VI GCNNNNNNNGC 1 cut(s) 2158
HpyF3I CTNAG 7 cut(s) 303, 488, 822, 905, 993, 1083, 1322
HpySE526I ACGT 6 cut(s) 124, 427, 696, 857, 1563, 1664
Hsp92I GRCGYC 2 cut(s) 124, 136
KflI GGGWCCC 1 cut(s) 1782
Kpn2I TCCGGA 3 cut(s) 1850, 1947, 2174
Kzo9I GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 1723, 2177
Lsp1109I GCAGC 1 cut(s) 2111
LweI GCATC 4 cut(s) 842, 1162, 1840, 2086
MaeI CTAG 9 cut(s) 128, 219, 614, 875, 887, 1113, 1835, 1889, 2356
MaeII ACGT 6 cut(s) 124, 427, 696, 857, 1563, 1664
MaeIII GTNAC 3 cut(s) 74, 1894, 2077
MalI GATC 8 cut(s) 308, 651, 690, 738, 1290, 1314, 1452, 1855
MboI GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
MfeI CAATTG 2 cut(s) 69, 1515
MhlI GDGCHC 3 cut(s) 1496, 1645, 1720
MlsI TGGCCA 1 cut(s) 733
MluNI TGGCCA 1 cut(s) 733
MlyI GAGTC 4 cut(s) 181, 952, 1496, 1610
MmeI TCCRAC 4 cut(s) 67, 840, 859, 963
Mox20I TGGCCA 1 cut(s) 733
Mph1103I ATGCAT 1 cut(s) 648
MroI TCCGGA 3 cut(s) 1850, 1947, 2174
MroXI GAANNNNTTC 3 cut(s) 834, 1410, 2243
MscI TGGCCA 1 cut(s) 733
MslI CAYNNNNRTG 1 cut(s) 273
Msp20I TGGCCA 1 cut(s) 733
MspCI CTTAAG 1 cut(s) 593
MspI CCGG 5 cut(s) 1851, 1911, 1948, 1982, 2175
MspR9I CCNGG 3 cut(s) 772, 1911, 1983
MunI CAATTG 2 cut(s) 69, 1515
Mva1269I GAATGC 2 cut(s) 648, 1114
MvaI CCWGG 1 cut(s) 772
MwoI GCNNNNNNNGC 1 cut(s) 2158
NciI CCSGG 2 cut(s) 1911, 1983
NdeII GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
NlaIV GGNNCC 5 cut(s) 34, 1494, 1719, 1783, 1784
NmeAIII GCCGAG 2 cut(s) 124, 2355
NmuCI GTSAC 2 cut(s) 1894, 2077
NsiI ATGCAT 1 cut(s) 648
NspI RCATGY 2 cut(s) 636, 1775
PagI TCATGA 1 cut(s) 309
PciI ACATGT 1 cut(s) 632
PciSI GCTCTTC 1 cut(s) 393
PctI GAATGC 2 cut(s) 648, 1114
PdmI GAANNNNTTC 3 cut(s) 834, 1410, 2243
PfeI GAWTC 5 cut(s) 169, 743, 1526, 1928, 2191
PfoI TCCNGGA 1 cut(s) 1909
PkrI GCNGC 1 cut(s) 2101
PleI GAGTC 4 cut(s) 181, 952, 1495, 1609
PpsI GAGTC 4 cut(s) 181, 952, 1495, 1609
PpuMI RGGWCCY 1 cut(s) 1782
PscI ACATGT 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 1782
Psp6I CCWGG 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 1653
PspGI CCWGG 1 cut(s) 770
PspN4I GGNNCC 5 cut(s) 34, 1494, 1719, 1783, 1784
PspOMI GGGCCC 1 cut(s) 1492
PspPI GGNCC 5 cut(s) 774, 1492, 1493, 1782, 1862
PspPPI RGGWCCY 1 cut(s) 1782
PstNI CAGNNNCTG 1 cut(s) 1175
RsaI GTAC 6 cut(s) 104, 657, 1577, 1582, 1593, 2276
RsaNI GTAC 6 cut(s) 103, 656, 1576, 1581, 1592, 2275
RseI CAYNNNNRTG 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 393
SatI GCNGC 1 cut(s) 2100
Sau3AI GATC 8 cut(s) 306, 649, 688, 736, 1288, 1312, 1450, 1853
Sau96I GGNCC 5 cut(s) 774, 1492, 1493, 1782, 1862
SchI GAGTC 4 cut(s) 181, 952, 1496, 1610
ScrFI CCNGG 3 cut(s) 772, 1911, 1983
SduI GDGCHC 3 cut(s) 1496, 1645, 1720
SfaNI GCATC 4 cut(s) 842, 1162, 1840, 2086
SinI GGWCC 2 cut(s) 774, 1782
SmiMI CAYNNNNRTG 1 cut(s) 273
SmlI CTYRAG 3 cut(s) 189, 593, 1048
SmoI CTYRAG 3 cut(s) 189, 593, 1048
SsiI CCGC 3 cut(s) 573, 967, 2227
SspI AATATT 3 cut(s) 1693, 1734, 2347
SspMI CTAG 9 cut(s) 128, 219, 614, 875, 887, 1113, 1835, 1889, 2356
StyD4I CCNGG 3 cut(s) 770, 1909, 1981
StyI CCWWGG 2 cut(s) 809, 921
TaaI ACNGT 7 cut(s) 37, 109, 566, 1064, 1580, 1754, 2090
TaiI ACGT 6 cut(s) 127, 430, 699, 860, 1566, 1667
TaqI TCGA 3 cut(s) 291, 1041, 1287
TatI WGTACW 2 cut(s) 102, 655
TfiI GAWTC 5 cut(s) 169, 743, 1526, 1928, 2191
TscAI CASTG 3 cut(s) 571, 1747, 2016
TseFI GTSAC 2 cut(s) 1894, 2077
TseI GCWGC 1 cut(s) 2099
Tsp45I GTSAC 2 cut(s) 1894, 2077
TspGWI ACGGA 4 cut(s) 7, 1543, 1913, 2300
TspRI CASTG 3 cut(s) 571, 1747, 2016
Vha464I CTTAAG 1 cut(s) 593
VpaK11BI GGWCC 2 cut(s) 774, 1782
XapI RAATTY 7 cut(s) 199, 326, 598, 1545, 1800, 1921, 1951
XbaI TCTAGA 1 cut(s) 1888
XceI RCATGY 2 cut(s) 636, 1775
XcmI CCANNNNNNNNNTGG 2 cut(s) 707, 1229
XmiI GTMKAC 1 cut(s) 39
XmnI GAANNNNTTC 3 cut(s) 834, 1410, 2243
XspI CTAG 9 cut(s) 128, 219, 614, 875, 887, 1113, 1835, 1889, 2356
ZraI GACGTC 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.