Rh4DG142800

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000042
Physical Location & Seq
Forward (+)
28279749 .. 28296784
17036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG142800.1

Sequence Viewer

Length: 4209 bp
ATGGCATTTCTTATTGATATTGGAACTGGAATTGTTGCAAAAATTGCGGACTACACGGTTGCACCAGTTGGGCGCCAAGTGGGCTATCTAATTTTTTACAACCGCAACCTCAAAAACCTAGATAGTCAACTGAGCGACTTAATTGCCATGAGACAGGATGTGGAGCAGAAGATTGGAGAAGAGAAGAGAAATAATAGAGAAGCGGAAGTCCATGTCAGCAAATGGGTGATAGATGCCAAGAGCATCACAAAAGAGGCAAACGATATCTTGAATGAAGAACATAAAGCAAAGGTGAAGTGTCTATGTGGATTATGTCCTAATCTAATGCTTCGTCATCAGTTAAGTCGAAAATCAACAAAATTGGTTCCCGACATTGCTGAACTCTATGCAAAAATTGATGTTAAAACTTTTGCACATGCTACCACCCCACAAGATGTACCCACCAAAGATTATGAGGCCTTCAGATCAAGGACTTTATTGGTGGACCAAATAATGGATGTATTGAGTAGTCCAAATATTGACATGATAGGAGTGTACGGGTTTGGTGGTGTGGGAAAGACCACACTTGTCAAAGAAGTTTCCCGGCAAGCTACAGAAGACAAAACATTATTTGATGATGTGGCTATGATATTGGATGTAAAACAAAATCCAAGCATCCCAAGAATTCAAAAAGAAATTGCTGAGAAGTTAGGTCTTGACCTTCCTGAAAATGAGACTGTTGCTGCAAGAGCACGCCGTCTATTTAGCAGGATAAAAGATCAAAAGACTCTCGTAATTTTAGATGATGTGTGGGAAAGAATTGATTTGGAAGCTGTGGGACTTTCTGGTGTGGCCACTTGTAAAGTATTGCTGACATCAAGAATGCGAGAAGTATTATCTCGTGAGATGCATACACAAAAAGAGTTTCCGCTTGATCTTTTAGGGGAAGAAGAAACATGGAATTTGTTCAAGAAGAAGGCAGGTGATGGTATTAAAGATCCTGCCATAAAAACTATAGCAACCAAAGTAGCCAAAAGATGTGGAGGTTTGCCGGTTTTAGTCGTCACTGTTGCCAGTGCCTTGAAAAATAGTACATTACATGCATGGAAAGATGCGTTGAGACGTTTGAAAAAGTTTGACAAAGAAGAATTCACAGAAAAGACATACTTGGCCCTAGAATGGAGTTATAGTCAGTTAAATGATGATGAGCTTAAGCCATTATTCTTGCTCTGTGGATTTTATACTCTTTCTCCAAAAACTATCCATATCCAATACCTATTCAAATATGGCATTGGTTTGGGTTTGTTTAAAAACCTCAATACACTGGGAGAAGCATGGGATGCATTAAATTCACAGCTTGAGAAACTGAAGAATTCTTGTCTAGTGCTTGACCATGAAGATAGTGGGTGTGTCAAAATGCATGATCTTGTGCATGATGTTGCTAAACGTATTTCATCAAGGGATAAGCAAGTGTTGAAAATAAGTGGTGGAGATGAGTTGAAAGAATGGCCAGACTGTGAAAAGTGTACTATGATATCTCTCCAAATGCTTAATATCCCTAAACTTCCTGAAGTTTTGCAATGTAAAGAACTAAAGTTGTTTCATCTTTACAATATTGCTAAGGATTTCTCCCTGGAAATCCCGTGCAACTTTTTTAAAGAAACGACAGAACTCGGAGTGTTGGATTTAACTTGTCTGAGTATCCCATCGCTACCTCAATCTCTTCAGTTCCTAAAAAATCTAAAGACATTGTGTTTAGACGCCTGCATCTTGGGTGACATTGCTCTAATTGGCCAGCTACATAACTTAGAAGTGCTTAGCTTTCTAGAATCCAAGTTTAAAGAGTTGCCCAAAGAAATAGGGCAATTGACTCATCTACGATCGTTGGATTTGACTAATTGCTCTCAACTGGAAGTAATTGAACCTAATGTTATATCAAGATTGAAAAGACTAGAAGAACTGAGGATGGGAAATAGCTTTAACCGATGGGAGGCTGAAGGAGTCATTAGTACTGAGAAAAATAGTGCAAGCCTTTCAGAGCTCAAGCACTTGTCTAAGCTAAGCATCTTGGATATACATATCTTGGATGCTAATATTCTTCCAACAGACTTGTTCTCCAATGCATTGGAAAGGTACCAAATATATATTGGTGCTGCATGGAACTGGGATGATGCTGATGCCAACCTCAACACATTGAAAATCGAGCTCACTACTACCAGCAATGAACTGTACCAATCTGGTTTAAATATGTTGTTGAAAAGAACCGATGAGTTACACTTAAATGGGATGGAGACTGATAATAATACTACCTATCAATTAGATGGCGAAGGATTTCAGCAACTGAAACATCTCCATGTCCACAATGCTGATTTTAGACATATCATTAATGGGAAGGGTGTTTTTCCCAACTTAAAAAGGTTGGTGGTGAGTGACCTAGACGGTTCGAGATTTTTATTATCATCTTCCATGGCTAGAAGTCTTGTACAACTCACACATCTTCAGATATCTGGATGTCAAACCATGGAAGAGATAGTATCAACAAACGAATCTAATGAAAAGCATATGAAGGACATGTTTTCTAAGCTACAACATTTGGAGCTAAAAGATCTTCCAATCCTCACAAGATTCTACTCAAGAAGTTATATTGGATTTCCGTCTTCGGGGATTTTTCAATTAGAGAACTACAATGAAATTGAAGAAAGGGACTCAATAGAAAACCTTGAGACTGCTTTGTTTGACGAAAAGGTAGTATTTCCAAGCTTGAAGAGATTGTTCATCAATGGCTTGACCAGGTTAACGACACTATGGCACAACCAACTTTCTCCAGAGAGTTTCAAAAATCTAGAGACAGTAGACGTTTGTTTCTGTCAAAGTTTGAAAAGTGTTTTTCCATCCTCAATGGTTAGAAGTCTAAAGCAGTTAATGCATCTGAAAGTGATTAACTGCGGAGTGGAGGAAATCATTGCAAATGAAGATGGACTACAAACAACACAGGAGTTTGTATTCTCAAAAGTAAACTCTGTCTTTTTTCGGGATTTGCCACATTTGAGGTGTTTCTATCCTGGAATGCATATTTCCTGGTGGCCGTCACTTGAAGACTTGGGGGTATATGGTTGCAGTAAAGTGGTGATTTTGGCCCAAGAATGTTCAAGCTTTGAGAGAAACTACGAGTCGAGGAGTCTTACACCAACTAACCAAGCTCTCTTGGTAATTAAGAAGGATTCATTCCCCAACTTGAAAGTAGCGGGCATGTCCGGTATGGAGATTTGGGATGGTCCATTCCCAGTAAAGTTCTTTAACAAACTAGAATTTCTTCGACTTGGTAATAAACAGTCCGAGTCTACTGCTGTACTTGAGAAATTCCTTGATCCCGATAAACTCAGTATAACGAGGTCTGCTGCTTCCAATGAAATATATTTCCATGAGAAAAGCAGTGGTGGAGATATAAATGCAGTTGGGACCCTCCCTCATTTAAGTGAGTTATGGCTTTGGGGCATGTGGAAGCTGAAGCATTTAGGGGAAGATGATTCCCAAATAGCAGGTCAAAATTTTCCGAAACTGAAAATTCTATGGATAAGTGATTGTAACAGCTTAAGGAATCTAAGATCATCAGCTATATCCTTCAAGAATCTGACAAAATTGCAAGTCACTGATTGTCGGAGATTGGAATACTTGTTAACTTACTCGATAGGTAAAAGTTTCATGCAACTCACATGCCTGATAGTTGAGGATTGTGCAAGACTGGTAGAAATAGTGGGTAGCACAAAAGAAGATAATGATTCAGGAAATGAGATTGTATTTAGCAAGTTGAAATATCTGAAACTTTCTAACCTACCAAGATTGCAAGGCTTTCGCTCTGGAGATTGGATTGTCAGATTCCCATCTTTGGAAACTTTAATCATAAGCAAACGCCTCAAGCTGAAGATTTTTCATAACGATGAAACGCTACAGGTGACAGAAGAAGAGGTTGACACAGACGTTGAAGCTGATGATGGTGCAACGGTACTAAAGGAAAAGATCGACATAGATACTGGTGGTGCTGATGGTGAACAAATTGATGAAACTACACAGTGCTCGGAGATGCCGCCATTGCTCATCGAATCCGGTGCGAGAGAAGTCCAGAACGCACACCTGTTTGAGAGGAAGACTGTGCGCTTAATGTGTGTACTATTGTCAGTGTGCATGCTACTGTTGGTGTTCGGGACTTACATGCGTGATTTGCTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1402

Amino Acids

159.07

Weight (kDa)

5.81

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 160 - 322 1e-31 NB-ARC domain
LRR_14 PF23598 512 - 811 1.2e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 774 - 874 1.4e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 897 - 976 1e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1075 - 1194 7.1e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1195 - 1300 1.9e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 950
Acc36I ACCTGC 2 cut(s) 950, 3509
Acc65I GGTACC 1 cut(s) 2112
AccB1I GGYRCC 2 cut(s) 72, 2112
AccB7I CCANNNNNTGG 1 cut(s) 493
AccI GTMKAC 2 cut(s) 2832, 3320
AciI CCGC 7 cut(s) 47, 103, 203, 908, 2925, 3224, 4064
AclWI GGATC 2 cut(s) 971, 3341
AcoI YGGCCR 4 cut(s) 831, 1487, 1771, 3062
AcsI RAATTY 9 cut(s) 663, 940, 1127, 1327, 1351, 3287, 3338, 3526, 3543
AcuI CTGAAG 8 cut(s) 445, 1367, 1569, 1688, 1995, 2462, 3506, 3920
AcyI GRCGYC 2 cut(s) 73, 1740
AfiI CCNNNNNNNGG 5 cut(s) 493, 1158, 1969, 2639, 3865
AflII CTTAAG 2 cut(s) 1190, 3571
AflIII ACRYGT 1 cut(s) 2550
AjnI CCWGG 4 cut(s) 1611, 2768, 3040, 3056
Alw21I GWGCWC 4 cut(s) 733, 2022, 2187, 4055
Alw26I GTCTC 6 cut(s) 145, 707, 1093, 2264, 2696, 2819
AlwI GGATC 2 cut(s) 971, 3341
AlwNI CAGNNNCTG 1 cut(s) 2320
AoxI GGCC 7 cut(s) 456, 831, 1149, 1487, 1771, 3062, 3114
ApeKI GCWGC 3 cut(s) 722, 2132, 3377
ApoI RAATTY 9 cut(s) 663, 940, 1127, 1327, 1351, 3287, 3338, 3526, 3543
ArsI GACNNNNNNTTYG 2 cut(s) 3505, 3537
AseI ATTAAT 1 cut(s) 2364
Asp700I GAANNNNTTC 3 cut(s) 944, 2310, 3291
Asp718I GGTACC 1 cut(s) 2112
AspLEI GCGC 2 cut(s) 75, 4134
AspS9I GGNCC 5 cut(s) 484, 1150, 3115, 3254, 3438
AsuC2I CCSGG 1 cut(s) 583
AsuHPI GGTGA 8 cut(s) 238, 304, 974, 1766, 2416, 3118, 3943, 4037
AvaII GGWCC 3 cut(s) 484, 3254, 3438
BalI TGGCCA 3 cut(s) 833, 1489, 1773
BanI GGYRCC 2 cut(s) 72, 2112
BanII GRGCYC 2 cut(s) 2022, 2187
BarI GAAGNNNNNNTAC 2 cut(s) 2943, 2975
BauI CACGAG 1 cut(s) 879
BbsI GAAGAC 4 cut(s) 603, 2628, 3081, 4130
Bbv12I GWGCWC 4 cut(s) 733, 2022, 2187, 4055
BbvI GCAGC 3 cut(s) 709, 2119, 3364
BceAI ACGGC 2 cut(s) 720, 3049
BcgI CGANNNNNNTGC 8 cut(s) 125, 159, 3305, 3339, 3812, 3846, 4067, 4101
BciT130I CCWGG 4 cut(s) 1613, 2770, 3042, 3058
BciVI GTATCC 1 cut(s) 1691
BcnI CCSGG 1 cut(s) 583
BcoDI GTCTC 6 cut(s) 145, 707, 1093, 2264, 2696, 2819
BfaI CTAG 9 cut(s) 119, 1154, 1361, 1805, 1931, 2414, 2451, 2822, 3284
BfmI CTRYAG 3 cut(s) 591, 993, 3926
BfoI RGCGCY 1 cut(s) 76
BfrI CTTAAG 2 cut(s) 1190, 3571
BfuAI ACCTGC 2 cut(s) 950, 3509
BfuI GTATCC 1 cut(s) 1691
BglI GCCNNNNNGGC 1 cut(s) 81
BglII AGATCT 1 cut(s) 2584
BisI GCNGC 4 cut(s) 723, 2133, 3378, 4064
BlpI GCTNAGC 2 cut(s) 1796, 2039
BlsI GCNGC 4 cut(s) 724, 2134, 3379, 4065
BmcAI AGTACT 1 cut(s) 1990
Bme1390I CCNGG 5 cut(s) 583, 1613, 2770, 3042, 3058
Bme18I GGWCC 3 cut(s) 484, 3254, 3438
BmgT120I GGNCC 5 cut(s) 484, 1150, 3115, 3254, 3438
BmiI GGNNCC 5 cut(s) 74, 366, 2114, 3439, 3440
BmrFI CCNGG 5 cut(s) 583, 1613, 2770, 3042, 3058
BmrI ACTGGG 3 cut(s) 1313, 2152, 3257
BmuI ACTGGG 3 cut(s) 1313, 2152, 3257
BpiI GAAGAC 4 cut(s) 603, 2628, 3081, 4130
BpmI CTGGAG 2 cut(s) 2787, 3858
Bpu10I CCTNAGC 1 cut(s) 1599
Bpu1102I GCTNAGC 2 cut(s) 1796, 2039
BpuEI CTTGAG 6 cut(s) 1358, 2006, 2596, 2720, 3353, 3878
BpuMI CCSGG 1 cut(s) 583
BsaBI GATNNNNATC 1 cut(s) 3859
BsaHI GRCGYC 2 cut(s) 73, 1740
BsaJI CCNNGG 3 cut(s) 1611, 2445, 2499
BsaWI WCCGGW 2 cut(s) 3233, 4082
BsaXI ACNNNNNCTCC 6 cut(s) 1153, 1183, 1213, 1243, 2078, 2108
Bsc4I CCNNNNNNNGG 5 cut(s) 493, 1158, 1969, 2639, 3865
Bse118I RCCGGY 1 cut(s) 1030
Bse1I ACTGG 9 cut(s) 31, 65, 1053, 1308, 1893, 2147, 3263, 3726, 4015
Bse3DI GCAATG 6 cut(s) 372, 1565, 1758, 2206, 2940, 4067
Bse8I GATNNNNATC 1 cut(s) 3859
BseBI CCWGG 4 cut(s) 1613, 2770, 3042, 3058
BseDI CCNNGG 3 cut(s) 1611, 2445, 2499
BseJI GATNNNNATC 1 cut(s) 3859
BseLI CCNNNNNNNGG 5 cut(s) 493, 1158, 1969, 2639, 3865
BseMI GCAATG 6 cut(s) 372, 1565, 1758, 2206, 2940, 4067
BseMII CTCAG 6 cut(s) 122, 672, 1667, 1931, 1983, 3373
BseNI ACTGG 9 cut(s) 31, 65, 1053, 1308, 1893, 2147, 3263, 3726, 4015
BseRI GAGGAG 1 cut(s) 3169
BseXI GCAGC 3 cut(s) 709, 2119, 3364
Bsh1285I CGRYCG 1 cut(s) 1862
BshFI GGCC 7 cut(s) 458, 833, 1151, 1489, 1773, 3064, 3116
BshNI GGYRCC 2 cut(s) 72, 2112
BsiEI CGRYCG 1 cut(s) 1862
BsiHKAI GWGCWC 4 cut(s) 733, 2022, 2187, 4055
BsiSI CCGG 4 cut(s) 583, 1031, 3234, 4083
BslFI GGGAC 4 cut(s) 831, 2696, 3451, 4195
BslI CCNNNNNNNGG 5 cut(s) 493, 1158, 1969, 2639, 3865
BsmAI GTCTC 6 cut(s) 145, 707, 1093, 2264, 2696, 2819
BsmBI CGTCTC 1 cut(s) 1093
BsmFI GGGAC 4 cut(s) 831, 2696, 3451, 4195
BsmI GAATGC 2 cut(s) 867, 3051
BsnI GGCC 7 cut(s) 458, 833, 1151, 1489, 1773, 3064, 3116
Bsp1286I GDGCHC 4 cut(s) 733, 2022, 2187, 4055
Bsp1407I TGTACA 1 cut(s) 2461
Bsp1720I GCTNAGC 2 cut(s) 1796, 2039
Bsp19I CCATGG 2 cut(s) 2445, 2499
BspACI CCGC 7 cut(s) 47, 103, 203, 908, 2925, 3224, 4064
BspANI GGCC 7 cut(s) 458, 833, 1151, 1489, 1773, 3064, 3116
BspCNI CTCAG 6 cut(s) 123, 673, 1668, 1932, 1984, 3372
BspLI GGNNCC 5 cut(s) 74, 366, 2114, 3439, 3440
BspMI ACCTGC 2 cut(s) 950, 3509
BspPI GGATC 2 cut(s) 971, 3341
BspT107I GGYRCC 2 cut(s) 72, 2112
BspTI CTTAAG 2 cut(s) 1190, 3571
BsrDI GCAATG 6 cut(s) 372, 1565, 1758, 2206, 2940, 4067
BsrFI RCCGGY 1 cut(s) 1030
BsrGI TGTACA 1 cut(s) 2461
BsrI ACTGG 9 cut(s) 31, 65, 1053, 1308, 1893, 2147, 3263, 3726, 4015
BssAI RCCGGY 1 cut(s) 1030
BssECI CCNNGG 3 cut(s) 1611, 2445, 2499
BssNI GRCGYC 2 cut(s) 73, 1740
BssSI CACGAG 1 cut(s) 879
BssT1I CCWWGG 2 cut(s) 2445, 2499
Bst2BI CACGAG 1 cut(s) 879
Bst2UI CCWGG 4 cut(s) 1613, 2770, 3042, 3058
Bst6I CTCTTC 6 cut(s) 174, 179, 1707, 2499, 2738, 3936
BstACI GRCGYC 2 cut(s) 73, 1740
BstAFI CTTAAG 2 cut(s) 1190, 3571
BstAPI GCANNNNNTGC 3 cut(s) 44, 1319, 2902
BstAUI TGTACA 1 cut(s) 2461
BstC8I GCNNGC 7 cut(s) 588, 733, 1744, 1775, 2008, 3226, 4163
BstDSI CCRYGG 2 cut(s) 2445, 2499
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 2 cut(s) 75, 4134
BstMAI GTCTC 6 cut(s) 145, 707, 1093, 2264, 2696, 2819
BstMCI CGRYCG 1 cut(s) 1862
BstMWI GCNNNNNNNGC 7 cut(s) 44, 81, 728, 1319, 2902, 4069, 4198
BstNI CCWGG 4 cut(s) 1613, 2770, 3042, 3058
BstNSI RCATGY 8 cut(s) 419, 1082, 2554, 3232, 3478, 3696, 4165, 4192
BstSCI CCNGG 5 cut(s) 581, 1611, 2768, 3040, 3056
BstSFI CTRYAG 3 cut(s) 591, 993, 3926
BstV1I GCAGC 3 cut(s) 709, 2119, 3364
BstV2I GAAGAC 4 cut(s) 603, 2628, 3081, 4130
BstX2I RGATCY 2 cut(s) 976, 2584
BstXI CCANNNNNNTGG 1 cut(s) 2104
BstYI RGATCY 2 cut(s) 976, 2584
BsuI GTATCC 1 cut(s) 1691
BsuRI GGCC 7 cut(s) 458, 833, 1151, 1489, 1773, 3064, 3116
BtgI CCRYGG 2 cut(s) 2445, 2499
BtgZI GCGATG 1 cut(s) 1671
BtsI GCAGTG 1 cut(s) 3418
BtsIMutI CAGTG 7 cut(s) 1044, 1060, 1301, 3418, 3627, 4055, 4161
BveI ACCTGC 2 cut(s) 950, 3509
Cac8I GCNNGC 7 cut(s) 588, 733, 1744, 1775, 2008, 3226, 4163
CaiI CAGNNNCTG 1 cut(s) 2320
CfoI GCGC 2 cut(s) 75, 4134
Cfr10I RCCGGY 1 cut(s) 1030
Cfr13I GGNCC 5 cut(s) 484, 1150, 3115, 3254, 3438
CseI GACGC 1 cut(s) 1748
CsiI ACCWGGT 1 cut(s) 2768
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
DinI GGCGCC 1 cut(s) 74
DraI TTTAAA 4 cut(s) 1288, 1636, 1819, 2223
EaeI YGGCCR 4 cut(s) 831, 1487, 1771, 3062
Eam1104I CTCTTC 6 cut(s) 174, 179, 1707, 2499, 2738, 3936
EarI CTCTTC 6 cut(s) 174, 179, 1707, 2499, 2738, 3936
Ecl136II GAGCTC 2 cut(s) 2020, 2185
Eco130I CCWWGG 2 cut(s) 2445, 2499
Eco147I AGGCCT 1 cut(s) 458
Eco24I GRGCYC 2 cut(s) 2022, 2187
Eco32I GATATC 3 cut(s) 265, 1515, 2484
Eco47I GGWCC 3 cut(s) 484, 3254, 3438
Eco53kI GAGCTC 2 cut(s) 2020, 2185
Eco57I CTGAAG 8 cut(s) 445, 1367, 1569, 1688, 1995, 2462, 3506, 3920
EcoICRI GAGCTC 2 cut(s) 2020, 2185
EcoO109I RGGNCCY 1 cut(s) 3438
EcoRI GAATTC 3 cut(s) 663, 1127, 1351
EcoRII CCWGG 4 cut(s) 1611, 2768, 3040, 3056
EcoRV GATATC 3 cut(s) 265, 1515, 2484
EcoT14I CCWWGG 2 cut(s) 2445, 2499
EcoT22I ATGCAT 7 cut(s) 891, 1084, 1324, 1401, 2104, 2907, 3051
EcoT38I GRGCYC 2 cut(s) 2022, 2187
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
ErhI CCWWGG 2 cut(s) 2445, 2499
Esp3I CGTCTC 1 cut(s) 1093
FalI AAGNNNNNCTT 2 cut(s) 1130, 1162
FaqI GGGAC 4 cut(s) 831, 2696, 3451, 4195
FauI CCCGC 1 cut(s) 3217
FauNDI CATATG 1 cut(s) 2541
FblI GTMKAC 2 cut(s) 2832, 3320
Fnu4HI GCNGC 4 cut(s) 723, 2133, 3378, 4064
FriOI GRGCYC 2 cut(s) 2022, 2187
Fsp4HI GCNGC 4 cut(s) 723, 2133, 3378, 4064
FspBI CTAG 9 cut(s) 119, 1154, 1361, 1805, 1931, 2414, 2451, 2822, 3284
GlaI GCGC 2 cut(s) 74, 4133
GluI GCNGC 4 cut(s) 723, 2133, 3378, 4064
GsuI CTGGAG 2 cut(s) 2787, 3858
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 7 cut(s) 458, 833, 1151, 1489, 1773, 3064, 3116
HapII CCGG 4 cut(s) 583, 1031, 3234, 4083
HgaI GACGC 1 cut(s) 1748
HhaI GCGC 2 cut(s) 75, 4134
Hin1I GRCGYC 2 cut(s) 73, 1740
Hin6I GCGC 2 cut(s) 73, 4132
HinP1I GCGC 2 cut(s) 73, 4132
HincII GTYRAC 4 cut(s) 128, 2775, 3657, 3949
HindII GTYRAC 4 cut(s) 128, 2775, 3657, 3949
HindIII AAGCTT 2 cut(s) 2737, 3130
HpaI GTTAAC 2 cut(s) 2775, 3657
HpaII CCGG 4 cut(s) 583, 1031, 3234, 4083
HphI GGTGA 8 cut(s) 238, 304, 974, 1766, 2416, 3118, 3943, 4037
HpyAV CCTTC 9 cut(s) 469, 710, 949, 1970, 2300, 2365, 2539, 3190, 3610
HpyCH4IV ACGT 4 cut(s) 1102, 1426, 2835, 3957
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 81, 728, 1319, 2902, 4069, 4198
HpySE526I ACGT 4 cut(s) 1102, 1426, 2835, 3957
Hsp92I GRCGYC 2 cut(s) 73, 1740
HspAI GCGC 2 cut(s) 73, 4132
KasI GGCGCC 1 cut(s) 72
KflI GGGWCCC 1 cut(s) 3438
KpnI GGTACC 1 cut(s) 2116
KspAI GTTAAC 2 cut(s) 2775, 3657
LmnI GCTCC 2 cut(s) 163, 2575
Lsp1109I GCAGC 3 cut(s) 709, 2119, 3364
MabI ACCWGGT 1 cut(s) 2768
MaeI CTAG 9 cut(s) 119, 1154, 1361, 1805, 1931, 2414, 2451, 2822, 3284
MaeII ACGT 4 cut(s) 1102, 1426, 2835, 3957
MaeIII GTNAC 8 cut(s) 1042, 1754, 2250, 2408, 3066, 3563, 3625, 3931
MfeI CAATTG 1 cut(s) 1844
MflI RGATCY 2 cut(s) 976, 2584
MhlI GDGCHC 4 cut(s) 733, 2022, 2187, 4055
MlsI TGGCCA 3 cut(s) 833, 1489, 1773
MluNI TGGCCA 3 cut(s) 833, 1489, 1773
Mly113I GGCGCC 1 cut(s) 73
MlyI GAGTC 7 cut(s) 760, 1843, 1989, 2678, 3158, 3166, 3326
MmeI TCCRAC 4 cut(s) 1641, 1845, 2105, 3617
Mox20I TGGCCA 3 cut(s) 833, 1489, 1773
Mph1103I ATGCAT 7 cut(s) 891, 1084, 1324, 1401, 2104, 2907, 3051
MroXI GAANNNNTTC 3 cut(s) 944, 2310, 3291
MscI TGGCCA 3 cut(s) 833, 1489, 1773
MslI CAYNNNNRTG 4 cut(s) 2259, 2331, 3453, 3915
Msp20I TGGCCA 3 cut(s) 833, 1489, 1773
MspCI CTTAAG 2 cut(s) 1190, 3571
MspI CCGG 4 cut(s) 583, 1031, 3234, 4083
MspR9I CCNGG 5 cut(s) 583, 1613, 2770, 3042, 3058
MunI CAATTG 1 cut(s) 1844
Mva1269I GAATGC 2 cut(s) 867, 3051
MvaI CCWGG 4 cut(s) 1613, 2770, 3042, 3058
MwoI GCNNNNNNNGC 7 cut(s) 44, 81, 728, 1319, 2902, 4069, 4198
NarI GGCGCC 1 cut(s) 73
NciI CCSGG 1 cut(s) 583
NcoI CCATGG 2 cut(s) 2445, 2499
NdeI CATATG 1 cut(s) 2541
NlaIV GGNNCC 5 cut(s) 74, 366, 2114, 3439, 3440
NmuCI GTSAC 6 cut(s) 1042, 1754, 2408, 3066, 3625, 3931
NsiI ATGCAT 7 cut(s) 891, 1084, 1324, 1401, 2104, 2907, 3051
NspI RCATGY 8 cut(s) 419, 1082, 2554, 3232, 3478, 3696, 4165, 4192
PaeI GCATGC 1 cut(s) 4165
PaqCI CACCTGC 1 cut(s) 950
PceI AGGCCT 1 cut(s) 458
PciI ACATGT 1 cut(s) 2550
PctI GAATGC 2 cut(s) 867, 3051
PdmI GAANNNNTTC 3 cut(s) 944, 2310, 3291
PflMI CCANNNNNTGG 1 cut(s) 493
PfoI TCCNGGA 1 cut(s) 3040
PkrI GCNGC 4 cut(s) 724, 2134, 3379, 4065
Ple19I CGATCG 1 cut(s) 1862
PleI GAGTC 7 cut(s) 760, 1843, 1988, 2678, 3157, 3165, 3325
PluTI GGCGCC 1 cut(s) 76
PpsI GAGTC 7 cut(s) 760, 1843, 1988, 2678, 3157, 3165, 3325
PpuMI RGGWCCY 1 cut(s) 3438
PscI ACATGT 1 cut(s) 2550
PshBI ATTAAT 1 cut(s) 2364
Psp124BI GAGCTC 2 cut(s) 2022, 2187
Psp5II RGGWCCY 1 cut(s) 3438
Psp6I CCWGG 4 cut(s) 1611, 2768, 3040, 3056
PspGI CCWGG 4 cut(s) 1611, 2768, 3040, 3056
PspN4I GGNNCC 5 cut(s) 74, 366, 2114, 3439, 3440
PspPI GGNCC 5 cut(s) 484, 1150, 3115, 3254, 3438
PspPPI RGGWCCY 1 cut(s) 3438
PstNI CAGNNNCTG 1 cut(s) 2320
PsuI RGATCY 2 cut(s) 976, 2584
PvuI CGATCG 1 cut(s) 1862
RseI CAYNNNNRTG 4 cut(s) 2259, 2331, 3453, 3915
SacI GAGCTC 2 cut(s) 2022, 2187
SatI GCNGC 4 cut(s) 723, 2133, 3378, 4064
Sau96I GGNCC 5 cut(s) 484, 1150, 3115, 3254, 3438
ScaI AGTACT 1 cut(s) 1990
SchI GAGTC 7 cut(s) 760, 1843, 1989, 2678, 3158, 3166, 3326
ScrFI CCNGG 5 cut(s) 583, 1613, 2770, 3042, 3058
SduI GDGCHC 4 cut(s) 733, 2022, 2187, 4055
SexAI ACCWGGT 1 cut(s) 2768
SfcI CTRYAG 3 cut(s) 591, 993, 3926
SfoI GGCGCC 1 cut(s) 74
SinI GGWCC 3 cut(s) 484, 3254, 3438
SmiMI CAYNNNNRTG 4 cut(s) 2259, 2331, 3453, 3915
SmlI CTYRAG 8 cut(s) 1190, 1337, 2021, 2611, 2699, 3332, 3571, 3893
SmoI CTYRAG 8 cut(s) 1190, 1337, 2021, 2611, 2699, 3332, 3571, 3893
SphI GCATGC 1 cut(s) 4165
SseBI AGGCCT 1 cut(s) 458
SsiI CCGC 7 cut(s) 47, 103, 203, 908, 2925, 3224, 4064
SspDI GGCGCC 1 cut(s) 72
SspI AATATT 3 cut(s) 517, 1594, 2074
SspMI CTAG 9 cut(s) 119, 1154, 1361, 1805, 1931, 2414, 2451, 2822, 3284
SstI GAGCTC 2 cut(s) 2022, 2187
StuI AGGCCT 1 cut(s) 458
StyD4I CCNGG 5 cut(s) 581, 1611, 2768, 3040, 3056
StyI CCWWGG 2 cut(s) 2445, 2499
TaiI ACGT 4 cut(s) 1105, 1429, 2838, 3960
TaqI TCGA 8 cut(s) 346, 2181, 2423, 3152, 3295, 3665, 3999, 4077
TatI WGTACW 6 cut(s) 1070, 1505, 1988, 2461, 3328, 4144
TauI GCSGC 1 cut(s) 4066
TscAI CASTG 7 cut(s) 1051, 1060, 1308, 3418, 3634, 4055, 4161
TseFI GTSAC 6 cut(s) 1042, 1754, 2408, 3066, 3625, 3931
TseI GCWGC 3 cut(s) 722, 2132, 3377
Tsp45I GTSAC 6 cut(s) 1042, 1754, 2408, 3066, 3625, 3931
TspGWI ACGGA 1 cut(s) 2622
TspRI CASTG 7 cut(s) 1051, 1060, 1308, 3418, 3634, 4055, 4161
Van91I CCANNNNNTGG 1 cut(s) 493
Vha464I CTTAAG 2 cut(s) 1190, 3571
VpaK11BI GGWCC 3 cut(s) 484, 3254, 3438
VspI ATTAAT 1 cut(s) 2364
XapI RAATTY 9 cut(s) 663, 940, 1127, 1327, 1351, 3287, 3338, 3526, 3543
XbaI TCTAGA 2 cut(s) 1804, 2821
XceI RCATGY 8 cut(s) 419, 1082, 2554, 3232, 3478, 3696, 4165, 4192
XcmI CCANNNNNNNNNTGG 2 cut(s) 1379, 2123
XmiI GTMKAC 2 cut(s) 2832, 3320
XmnI GAANNNNTTC 3 cut(s) 944, 2310, 3291
XspI CTAG 9 cut(s) 119, 1154, 1361, 1805, 1931, 2414, 2451, 2822, 3284
ZrmI AGTACT 1 cut(s) 1990
Zsp2I ATGCAT 7 cut(s) 891, 1084, 1324, 1401, 2104, 2907, 3051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.