Rroxscaffold_4G00299140

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000042
Physical Location & Seq
Reverse (-)
19062537 .. 19068991
6455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299140.1

Sequence Viewer

Length: 2811 bp
ATGCCAAAACTCAAGGTGTTGGATATTTGCAATCTGCCTCTTATGTCACTACCTCCATCTCTTGAATTGCTGAAAACCAATCTTCAAACATTGTGTTTAGATGGATGCACGTTGGAAGACATAGCTCGAGTCGGAGAGCTAAGCAACTTGGAAACTCTTAGCTTTGCTAACTCCAAGGTCAAAGAGCTGCCAAAAGAAATAGCGAAATTGACTCATCTGCGGTTGTTAAACTTGTCGGATTGCCCTGAGCTTGAAGTGATTTCACCTAATGTCATATCAAGTTTGACAAGGCTCGAAGACTTGAGAATGAAAAACAGTTTTGAAAAATGGGAGGCTGAGGGAGACATCAACAAAAGAAGGAATGCCAGTCTTTCAGAGCTGAAGGACTTATCGCAATTAACTGCATTAGAAATATATATTCCAGATGCTGATATTCTTCCACCAAACTTGTTCTCCAATAAATTGGAGACATACCATATATATATTGGTGGTGTGTGGAAAAGGAGGCATATGTGGGGAAAATTTATGGCTGAAACCACCGTCAATACACTAAAGCTCGAGCTCACTCATAGCAATCAATGGGATCAAGATCTAAAAAGACTGATGAAGAGATCTGAAGATTTGGCCTTGGATGGGAAGGAAGGTGTTAATGATAGTTTGTATCAAGTAGACAACGAAGGTTTTCCGCAGCTGAAACATCTCCGACTCCAAAACAATATTGATTTTACATATACAATTGGCAGGAAGGCTGTGTTCCCCAGCTTAACAACCTTGAGCATACAAAAATGTTATCATTTAACATTCTTGTTCTCATGTTCAATGGCTAAAAGTTTCGAACAGCTCAAACATCTTGAGATATGCAACTGCAACAGCATGGAAGAAGTAGTATCATCAACAACAGAGTACAATGAAGAAAATATGAATAACATGTTCTGCAAGCTTGAAACTCTAGAACTGGAGGAACTTCCATGCCTCGGTAGAATTTGCACAGGAACTCGTATTGAATTTCCATTCTTGAAGAGATTGGTTATAAAGTATTGTGAAAAATTGAAGGCGTTCATCTGTGATCCGATGAGTAATAAAAGTACTAGAATCTGCGAAGAAATTGAAGAGAGGGAGTCAGAGGAGAGCCCAAGTGTTGAACATGAGAGTGTTGCAGAACACTGCCTTTTTAACGACAAGGTAAGATTTCCTAGCTTGGAGGAATTGAAAATAGATGCTTTAGACGAGTTGCAGACAATATGGCACAATAACCAACTTGCTGATCAAGACTCTTTTTGCCGACTCAAACAAGTTTACGTTAGATATTGCAATAGTCTGATAAGTATATTTCCGCCTAGTATCATGGGGAGATTGAATGCTCTGGAAAGATTAAGCATAGGAAGTTGCGGCTCACTTAAAGTGGTATTTGAAGTCGGAGGAATTAATGTTGAAGAGACACGTGTCAACAGATCAACCCCTTATGATCAGTGGAAACCTTTGTACTGTCAAAATCTTAAATTCTTGAGCATATGGCGAGCTGACAGTTTGAAAAATATATTCCCAGCCTCTTTGGCCAGAGGACTTCAGCAGCTACGATATCTGAGGGTGCTCTCTTGTCGCGATCTGCTGGAAATAGTTGGTGAGGAAGAGGGAGGAGTAGAATTACCAACGGTTCCTCAGTTTGTGTTCCCAAAGGCAACAGACGTGGAATTGGTAGGCCTGCCCCAACTTAAGAGTTTTTATCCGGGAAAGCATACTTCCAAGTGGCCGTTACTCGAAACATTGGAAGTATCCCAAAATTATAAGGTGGAACTTTCAGTCTTTCAGGATAAACATGAGTCGGGCCCAGTGAAGTCTCTCTTTTCAATTGACAAGGATTCATTCCCCAACTTGGAAGTATTGTACGTGGGCAACGTGGAAATTTGGTACGGTACATCCCCAGTACCAGTAGAGTCCTTCAGCAAACTAAAATATATTAGTTTTGAACATCGGGCACCTTTCTCCCAGCAAGCTACATCATTCTTCCTTCAGCAATTACACAATATTGAAAGAATTAAAGTGAATTATGCTCCTCCCGAAGAAATATTTCTCAGTGAAGGAAACAGTACTTGTGAGGAAATAAATGCAGTTGGGACCCTACCACGCATACGAATTTTAGAGTTCTATAGAAGCGAAATGTTGCATCTTGGGAAAGAGAACTCCCGATCAGTGAGTCCAATTTTTCCAAAGTTGGAAGTTCTAGAGGTGAGGCGGCATTCTCCCGGACTAAGGAATTTGGAGTCATCAGCTATATCCTTCCGGAATTTAACAACTTTGAAAGTAGTTTATTGTCCGGGTTTGGAGCATTTAATAACTTATTCAGTGGCTAAAAGCTTAACGCGACTGACAACAATGAAAGTGAAATATTGTCCAAATATTGCAGTAATAGTGGCAATAGACGGAGATGATGCAGCAAACGAGATTACTTTGAACAGGTTGCAAGTTTTAACCCTTCGTGGACTGCCAAAAATGCAAGGCTTTTGCTCCGGAAGTTGCATTGTGAAACTCCCATCCTTGACTAGTTCATCCATGAGCAACCGCCTCAAATTAAAGATTTTCGGTGATGATGGAAAGCTAACGATAACAAGCAAGCACTCTGACACGGAGGAGGAGGAGGAGAAGGAGAAGCAGGAGCTGGAGGAAATAGTGAGTCTGCAAGCTGCTGCCTCAAGGAAAGCAGTACAGAAGGCACACCCTGTTTCAGTGGAAGGACGCTCTACTACGTGTTCGAGAAATGGTTGTGGGAGTTGTAGAGGCATTGATGCGTTCCTAGTTACTTTGCTGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

936

Amino Acids

106.33

Weight (kDa)

5.89

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 297 2.8e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 249 - 352 1.6e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 400 - 481 7.8e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 494 - 534 8.1e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 634 - 797 2.6e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 752 - 836 2.6e-08 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000042)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07760 FvH4_1g18111 FvH4_1g18121 FvH4_1g18142 FvH4_1g18160 FvH4_3g04780 FvH4_3g04780 FvH4_3g04780 FvH4_3g05070 FvH4_3g29991 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06020 FvH4_4g06021 FvH4_4g06022 FvH4_4g10330 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g02660 FvH4_5g05380 FvH4_5g18950 FvH4_5g18991 FvH4_5g18992 FvH4_6g53580 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140 FvH4_7g31140
malus_domestica MD08G1082300.v1.1 MD08G1083200.v1.1 MD08G1083600.v1.1 MD08G1083700.v1.1 MD09G1272000.v1.1 MD09G1272400.v1.1 MD09G1272500.v1.1 MD09G1272700.v1.1 MD09G1272800.v1.1 MD09G1273400.v1.1 MD09G1273700.v1.1 MD09G1274000.v1.1 MD09G1274100.v1.1 MD09G1274200.v1.1 MD09G1274400.v1.1 MD11G1203100.v1.1 MD13G1197600.v1.1 MD15G1069900.v1.1 MD15G1070000.v1.1
prunus_persica Prupe.3G016600_v2.0.a1 Prupe.3G016700_v2.0.a1 Prupe.4G236500_v2.0.a1
pyrus_communis pycom08g06810 pycom08g06820 pycom08g06830 pycom08g06850 pycom08g06860 pycom08g06870 pycom08g06900 pycom08g06910 pycom08g06920 pycom08g06950 pycom09g18250 pycom09g18300 pycom09g18310 pycom09g18340 pycom09g18360 pycom09g18390 pycom09g18410 pycom09g18420 pycom09g18470 pycom09g18510 pycom11g17610 pycom15g06550 pycom15g06560 pycom15g06570 pycom15g06590 pycom15g06600
rosa_chinensis RchiOBHm_Chr1g0356991 RchiOBHm_Chr1g0357001 RchiOBHm_Chr2g0085061 RchiOBHm_Chr2g0085071 RchiOBHm_Chr2g0085111 RchiOBHm_Chr2g0085121 RchiOBHm_Chr2g0108611 RchiOBHm_Chr2g0109281 RchiOBHm_Chr4g0396311 RchiOBHm_Chr4g0397551 RchiOBHm_Chr4g0397781 RchiOBHm_Chr4g0397831 RchiOBHm_Chr4g0397841 RchiOBHm_Chr4g0397871 RchiOBHm_Chr4g0397911 RchiOBHm_Chr4g0397921 RchiOBHm_Chr4g0398201 RchiOBHm_Chr4g0398211 RchiOBHm_Chr4g0408401 RchiOBHm_Chr5g0016101 RchiOBHm_Chr5g0016111 RchiOBHm_Chr5g0016321 RchiOBHm_Chr5g0016331 RchiOBHm_Chr5g0016341 RchiOBHm_Chr5g0016351 RchiOBHm_Chr7g0203341 RchiOBHm_Chr7g0203371 RchiOBHm_Chr7g0203421 RchiOBHm_Chr7g0204211 RchiOBHm_Chr7g0204231 RchiOBHm_Chr7g0236741
rosa_laevigata RLG00000001063 RLG00000009379 RLG00000009397 RLG00000009399 RLG00000009402 RLG00000009403 RLG00000009407 RLG00000009411 RLG00000009429 RLG00000009550 RLG00000009551 RLG00000015657 RLG00000017681 RLG00000028063 RLG00000028065 RLG00000028066 RLG00000032242 RLG00000032245 RLG00000035025 RLG00000035026 RLG00000035027 RLG00000035031
rosa_multiflora Rmu_co7992674.1_g000001 Rmu_co8252071.1_g000001 Rmu_co8481849.1_g000001 Rmu_sc0000166.1_g000026 Rmu_sc0000166.1_g000032 Rmu_sc0000166.1_g000036 Rmu_sc0000299.1_g000001 Rmu_sc0000332.1_g000001 Rmu_sc0000361.1_g000022 Rmu_sc0000361.1_g000023 Rmu_sc0000361.1_g000024 Rmu_sc0000361.1_g000034 Rmu_sc0000361.1_g000053 Rmu_sc0000571.1_g000009 Rmu_sc0000777.1_g000005 Rmu_sc0000777.1_g000013 Rmu_sc0000787.1_g000002 Rmu_sc0000795.1_g000061 Rmu_sc0000795.1_g000068 Rmu_sc0001074.1_g000012 Rmu_sc0001074.1_g000024 Rmu_sc0001196.1_g000010 Rmu_sc0001196.1_g000013 Rmu_sc0001235.1_g000035 Rmu_sc0001320.1_g000009 Rmu_sc0001320.1_g000010 Rmu_sc0001588.1_g000017 Rmu_sc0001779.1_g000028 Rmu_sc0001807.1_g000009 Rmu_sc0001959.1_g000001 Rmu_sc0001959.1_g000003 Rmu_sc0001959.1_g000004 Rmu_sc0001959.1_g000025 Rmu_sc0002148.1_g000007 Rmu_sc0002148.1_g000013 Rmu_sc0002212.1_g000007 Rmu_sc0002321.1_g000015 Rmu_sc0002460.1_g000018 Rmu_sc0002540.1_g000008 Rmu_sc0002647.1_g000001 Rmu_sc0003347.1_g000005 Rmu_sc0003347.1_g000013 Rmu_sc0003582.1_g000009 Rmu_sc0003720.1_g000025 Rmu_sc0004527.1_g000001 Rmu_sc0004557.1_g000001 Rmu_sc0004595.1_g000015 Rmu_sc0004595.1_g000020 Rmu_sc0004834.1_g000001 Rmu_sc0004904.1_g000029 Rmu_sc0005276.1_g000007 Rmu_sc0005276.1_g000008 Rmu_sc0005276.1_g000010 Rmu_sc0005276.1_g000019 Rmu_sc0005506.1_g000016 Rmu_sc0005723.1_g000003 Rmu_sc0005766.1_g000006 Rmu_sc0006749.1_g000001 Rmu_sc0006749.1_g000002 Rmu_sc0006985.1_g000001 Rmu_sc0007032.1_g000011 Rmu_sc0007315.1_g000007 Rmu_sc0007574.1_g000003 Rmu_sc0008179.1_g000001 Rmu_sc0008179.1_g000002 Rmu_sc0008208.1_g000001 Rmu_sc0008647.1_g000014 Rmu_sc0008647.1_g000016 Rmu_sc0009960.1_g000001 Rmu_sc0010313.1_g000013 Rmu_sc0010376.1_g000001 Rmu_sc0012840.1_g000006 Rmu_sc0013807.1_g000003 Rmu_sc0013807.1_g000004 Rmu_sc0013807.1_g000005 Rmu_sc0014155.1_g000001 Rmu_sc0015959.1_g000003 Rmu_sc0015959.1_g000004 Rmu_sc0016628.1_g000001 Rmu_sc0018579.1_g000001 Rmu_sc0019842.1_g000001 Rmu_sc0022243.1_g000001 Rmu_sc0022243.1_g000002 Rmu_sc0023640.1_g000001 Rmu_sc0028411.1_g000001 Rmu_sc0033723.1_g000001 Rmu_sc0038038.1_g000001 Rmu_sc0039543.1_g000001 Rmu_sc0040505.1_g000001 Rmu_sc0043049.1_g000001 Rmu_ssc0000234.1_g000013
rosa_roxburghii Rroxscaffold_1G00024370 Rroxscaffold_1G00024400 Rroxscaffold_1G00024420 Rroxscaffold_1G00039110 Rroxscaffold_1G00060530 Rroxscaffold_1G00060560 Rroxscaffold_2G00132370 Rroxscaffold_2G00134940 Rroxscaffold_2G00134960 Rroxscaffold_2G00155630 Rroxscaffold_3G00253360 Rroxscaffold_3G00253840 Rroxscaffold_3G00253850 Rroxscaffold_4G00299140 Rroxscaffold_4G00299150 Rroxscaffold_5G00341140 Rroxscaffold_5G00341150 Rroxscaffold_5G00342380 Rroxscaffold_5G00342390 Rroxscaffold_5G00342580 Rroxscaffold_5G00342590 Rroxscaffold_5G00342600 Rroxscaffold_5G00342620 Rroxscaffold_5G00342640 Rroxscaffold_5G00342670 Rroxscaffold_5G00342680 Rroxscaffold_5G00342720 Rroxscaffold_5G00342750 Rroxscaffold_5G00342760 Rroxscaffold_5G00342770 Rroxscaffold_5G00342780 Rroxscaffold_5G00342790 Rroxscaffold_5G00342820 Rroxscaffold_5G00342880
rosa_rugosa Rorug01G0064000 Rorug01G0252600 Rorug01G0252700 Rorug01G0252900 Rorug01G0253000 Rorug01G0457800 Rorug02G0154700 Rorug02G0154800 Rorug02G0159000 Rorug03G0342700 Rorug03G0350300 Rorug04G0004400 Rorug05G0031200 Rorug05G0031300 Rorug05G0031400 Rorug05G0031800 Rorug05G0295400 Rorug05G0295500 Rorug07G0075800.1 Rorug07G0075900 Rorug07G0076000
rosa_samantha Rh1AG267100 Rh1BG234600 Rh1BG234800 Rh1BG234900 Rh1CG250500 Rh1CG250900 Rh1CG251100 Rh1DG262000 Rh1DG262200 Rh1DG262300 Rh2AG005800 Rh2AG006000 Rh2AG203700 Rh2AG204000 Rh2AG204400 Rh2AG210500 Rh2AG210600 Rh2BG006700 Rh2BG216500 Rh2BG220800 Rh2CG007100 Rh2CG007200 Rh2CG007400 Rh2CG007500 Rh2CG207700 Rh2CG212800 Rh2DG007100 Rh2DG007500 Rh2DG210800 Rh2DG216700 Rh2DG216800 Rh4AG078900 Rh4AG079000 Rh4AG080700 Rh4AG081300 Rh4AG084000 Rh4AG150000 Rh4BG066400 Rh4BG074400 Rh4BG074500 Rh4BG074900 Rh4BG075200 Rh4BG075300 Rh4BG075900 Rh4BG076700 Rh4BG078500 Rh4BG078600 Rh4BG078700 Rh4BG081500 Rh4CG073200 Rh4CG081000 Rh4CG084300 Rh4CG084400 Rh4CG084500 Rh4CG087200 Rh4CG087300 Rh4CG087800 Rh4CG088200 Rh4CG088900 Rh4CG091200 Rh4CG157300 Rh4DG063300 Rh4DG073700 Rh4DG074000 Rh4DG074100 Rh4DG074200 Rh4DG074300 Rh4DG142800 Rh5AG124600 Rh5AG125000 Rh5AG125100 Rh5AG125200 Rh5AG125300 Rh5AG367000 Rh5AG367100 Rh5AG367200 Rh5AG367300 Rh5AG367400 Rh5BG123600 Rh5BG123800 Rh5BG124300 Rh5BG377200 Rh5BG377500 Rh5CG135300 Rh5CG136400 Rh5CG136500 Rh5CG400600 Rh5CG400700 Rh5CG400800 Rh5DG122900 Rh5DG123000 Rh5DG124000 Rh5DG389900 Rh5DG390200 Rh5DG390700 Rh5DG390800 Rh7AG205300 Rh7AG210900 Rh7AG211500 Rh7AG221400 Rh7BG205100 Rh7BG209200 Rh7BG209400 Rh7BG425000 Rh7BG425100 Rh7CG216600 Rh7CG217200 Rh7CG217300 Rh7CG217700 Rh7DG115300 Rh7DG211800 Rh7DG212400 Rh7DG217600 Rh7DG218400
rosa_wichuraiana Rw0G002820 Rw0G003970 Rw0G003980 Rw0G015020 Rw0G018950 Rw0G020820 Rw0G021460 Rw0G021470 Rw1G023590 Rw1G023600 Rw2G000530 Rw2G000550 Rw2G016290 Rw4G005460 Rw4G006420 Rw4G006430 Rw4G006620 Rw4G006660 Rw4G006670 Rw4G006700 Rw4G006710 Rw4G006750 Rw4G006990 Rw4G007000 Rw4G012240 Rw5G010800 Rw5G010840 Rw5G010860 Rw5G034410 Rw5G034450 Rw5G034460 Rw7G017900 Rw7G017910 Rw7G017930 Rw7G019140 Rw7G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1031, 1785
AccB1I GGYRCC 1 cut(s) 1975
AccI GTMKAC 1 cut(s) 669
AccII CGCG 2 cut(s) 1602, 2362
AccIII TCCGGA 2 cut(s) 2280, 2507
AciI CCGC 6 cut(s) 220, 686, 1334, 1389, 2233, 2560
AclWI GGATC 2 cut(s) 591, 1061
AcoI YGGCCR 2 cut(s) 1554, 1748
AcsI RAATTY 8 cut(s) 521, 981, 1004, 1499, 1902, 2133, 2254, 2284
AcuI CTGAAG 5 cut(s) 401, 636, 1550, 1924, 1994
AcvI CACGTG 1 cut(s) 1442
AfaI GTAC 9 cut(s) 905, 1087, 1484, 1886, 1910, 1915, 1926, 2089, 2703
AfiI CCNNNNNNNGG 4 cut(s) 633, 974, 1873, 2250
AflII CTTAAG 1 cut(s) 1712
AflIII ACRYGT 4 cut(s) 927, 1439, 1441, 2744
AhlI ACTAGT 1 cut(s) 2540
AjiI CACGTC 1 cut(s) 1687
Alw21I GWGCWC 2 cut(s) 564, 1593
Alw26I GTCTC 4 cut(s) 336, 461, 1430, 1842
AlwI GGATC 2 cut(s) 591, 1061
AlwNI CAGNNNCTG 2 cut(s) 428, 2656
Ama87I CYCGRG 2 cut(s) 126, 557
Aor13HI TCCGGA 2 cut(s) 2280, 2507
AoxI GGCC 5 cut(s) 624, 1554, 1699, 1748, 1825
ApaI GGGCCC 1 cut(s) 1829
ApeKI GCWGC 6 cut(s) 187, 688, 1570, 2432, 2681, 2684
ApoI RAATTY 8 cut(s) 521, 981, 1004, 1499, 1902, 2133, 2254, 2284
AseI ATTAAT 1 cut(s) 1425
Asp700I GAANNNNTTC 3 cut(s) 681, 1055, 2067
AspS9I GGNCC 3 cut(s) 1825, 1826, 2115
AsuC2I CCSGG 3 cut(s) 1728, 2244, 2316
AsuHPI GGTGA 4 cut(s) 255, 1634, 2239, 2594
AsuII TTCGAA 1 cut(s) 834
AvaI CYCGRG 2 cut(s) 126, 557
AvaII GGWCC 1 cut(s) 2115
BaeGI GKGCMC 2 cut(s) 1829, 1978
BalI TGGCCA 1 cut(s) 1556
BanI GGYRCC 1 cut(s) 1975
BanII GRGCYC 3 cut(s) 564, 1133, 1829
BbrPI CACGTG 1 cut(s) 1442
BbsI GAAGAC 2 cut(s) 123, 303
Bbv12I GWGCWC 2 cut(s) 564, 1593
BbvCI CCTCAGC 1 cut(s) 336
BbvI GCAGC 6 cut(s) 174, 700, 1582, 2444, 2668, 2671
BccI CCATC 5 cut(s) 64, 95, 626, 2539, 2582
BceAI ACGGC 1 cut(s) 1735
BciVI GTATCC 1 cut(s) 1783
BclI TGATCA 2 cut(s) 1264, 1465
BcnI CCSGG 3 cut(s) 1728, 2244, 2316
BcoDI GTCTC 4 cut(s) 336, 461, 1430, 1842
BcuI ACTAGT 1 cut(s) 2540
BfaI CTAG 7 cut(s) 950, 1089, 1194, 1338, 2222, 2541, 2792
BfmI CTRYAG 1 cut(s) 2146
BfrI CTTAAG 1 cut(s) 1712
BfuI GTATCC 1 cut(s) 1783
BglI GCCNNNNNGGC 1 cut(s) 1553
BglII AGATCT 2 cut(s) 589, 611
BisI GCNGC 8 cut(s) 188, 689, 1390, 1571, 2234, 2433, 2682, 2685
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 8 cut(s) 189, 690, 1391, 1572, 2235, 2434, 2683, 2686
BmcAI AGTACT 2 cut(s) 1087, 2089
Bme1390I CCNGG 3 cut(s) 1728, 2244, 2316
Bme18I GGWCC 1 cut(s) 2115
BmeT110I CYCGRG 2 cut(s) 126, 557
BmgBI CACGTC 1 cut(s) 1687
BmgT120I GGNCC 3 cut(s) 1825, 1826, 2115
BmiI GGNNCC 5 cut(s) 1656, 1827, 1977, 2116, 2117
BmrFI CCNGG 3 cut(s) 1728, 2244, 2316
BmrI ACTGGG 2 cut(s) 1823, 1916
BmsI GCATC 6 cut(s) 95, 415, 1207, 2173, 2419, 2773
BmuI ACTGGG 2 cut(s) 1823, 1916
BoxI GACNNNNGTC 1 cut(s) 1442
BpiI GAAGAC 2 cut(s) 123, 303
BpmI CTGGAG 2 cut(s) 977, 2678
Bpu10I CCTNAGC 2 cut(s) 246, 336
Bpu1102I GCTNAGC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 5 cut(s) 322, 793, 872, 1525, 2674
BpuMI CCSGG 3 cut(s) 1728, 2244, 2316
BsaAI YACGTR 3 cut(s) 1442, 1888, 2745
BsaBI GATNNNNATC 1 cut(s) 588
BsaJI CCNNGG 3 cut(s) 174, 627, 973
BsaWI WCCGGW 2 cut(s) 2280, 2507
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 4 cut(s) 633, 974, 1873, 2250
Bse1I ACTGG 5 cut(s) 366, 960, 1829, 1922, 1928
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 2 cut(s) 2280, 2507
BseDI CCNNGG 3 cut(s) 174, 627, 973
BseGI GGATG 5 cut(s) 110, 637, 1916, 2531, 2546
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 4 cut(s) 633, 974, 1873, 2250
BseMII CTCAG 5 cut(s) 237, 327, 1574, 1673, 2086
BseNI ACTGG 5 cut(s) 366, 960, 1829, 1922, 1928
BseRI GAGGAG 7 cut(s) 1139, 1650, 2043, 2642, 2645, 2648, 2651
BseSI GKGCMC 2 cut(s) 1829, 1978
BseXI GCAGC 6 cut(s) 174, 700, 1582, 2444, 2668, 2671
BseYI CCCAGC 3 cut(s) 758, 1543, 1986
Bsh1236I CGCG 2 cut(s) 1602, 2362
BshFI GGCC 5 cut(s) 626, 1556, 1701, 1750, 1827
BshNI GGYRCC 1 cut(s) 1975
BsiHKAI GWGCWC 2 cut(s) 564, 1593
BsiHKCI CYCGRG 2 cut(s) 126, 557
BsiSI CCGG 5 cut(s) 1727, 2244, 2281, 2315, 2508
BslFI GGGAC 1 cut(s) 2128
BslI CCNNNNNNNGG 4 cut(s) 633, 974, 1873, 2250
BsmAI GTCTC 4 cut(s) 336, 461, 1430, 1842
BsmFI GGGAC 1 cut(s) 2128
BsmI GAATGC 3 cut(s) 367, 1363, 2236
BsnI GGCC 5 cut(s) 626, 1556, 1701, 1750, 1827
BsoBI CYCGRG 2 cut(s) 126, 557
Bsp119I TTCGAA 1 cut(s) 834
Bsp120I GGGCCC 1 cut(s) 1825
Bsp1286I GDGCHC 5 cut(s) 564, 1133, 1593, 1829, 1978
Bsp13I TCCGGA 2 cut(s) 2280, 2507
Bsp143I GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp68I TCGCGA 1 cut(s) 1602
BspACI CCGC 6 cut(s) 220, 686, 1334, 1389, 2233, 2560
BspANI GGCC 5 cut(s) 626, 1556, 1701, 1750, 1827
BspCNI CTCAG 5 cut(s) 238, 328, 1575, 1672, 2085
BspEI TCCGGA 2 cut(s) 2280, 2507
BspFNI CGCG 2 cut(s) 1602, 2362
BspLI GGNNCC 5 cut(s) 1656, 1827, 1977, 2116, 2117
BspPI GGATC 2 cut(s) 591, 1061
BspT104I TTCGAA 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 1975
BspTI CTTAAG 1 cut(s) 1712
BsrI ACTGG 5 cut(s) 366, 960, 1829, 1922, 1928
BssECI CCNNGG 3 cut(s) 174, 627, 973
BssMI GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
BssT1I CCWWGG 2 cut(s) 174, 627
Bst4CI ACNGT 7 cut(s) 317, 541, 1487, 1526, 1654, 1913, 2087
Bst6I CTCTTC 5 cut(s) 602, 1013, 1104, 1428, 1623
BstAFI CTTAAG 1 cut(s) 1712
BstBAI YACGTR 3 cut(s) 1442, 1888, 2745
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 6 cut(s) 938, 1518, 1703, 1992, 2612, 2679
BstDEI CTNAG 8 cut(s) 140, 158, 246, 336, 1583, 1659, 2072, 2249
BstF5I GGATG 5 cut(s) 110, 637, 1916, 2531, 2546
BstFNI CGCG 2 cut(s) 1602, 2362
BstKTI GATC 9 cut(s) 586, 592, 614, 1069, 1267, 1454, 1468, 1606, 2189
BstMAI GTCTC 4 cut(s) 336, 461, 1430, 1842
BstMBI GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
BstMWI GCNNNNNNNGC 2 cut(s) 1553, 2491
BstNSI RCATGY 1 cut(s) 931
BstPAI GACNNNNGTC 1 cut(s) 1442
BstSCI CCNGG 3 cut(s) 1726, 2242, 2314
BstSFI CTRYAG 1 cut(s) 2146
BstSLI GKGCMC 2 cut(s) 1829, 1978
BstUI CGCG 2 cut(s) 1602, 2362
BstV1I GCAGC 6 cut(s) 174, 700, 1582, 2444, 2668, 2671
BstV2I GAAGAC 2 cut(s) 123, 303
BstX2I RGATCY 2 cut(s) 589, 611
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 2 cut(s) 589, 611
BsuI GTATCC 1 cut(s) 1783
BsuRI GGCC 5 cut(s) 626, 1556, 1701, 1750, 1827
BtrI CACGTC 1 cut(s) 1687
BtsCI GGATG 5 cut(s) 110, 637, 1916, 2531, 2546
BtsI GCAGTG 1 cut(s) 1162
BtsIMutI CAGTG 7 cut(s) 1162, 1475, 1836, 2080, 2196, 2349, 2730
BtuMI TCGCGA 1 cut(s) 1602
Cac8I GCNNGC 6 cut(s) 938, 1518, 1703, 1992, 2612, 2679
CaiI CAGNNNCTG 2 cut(s) 428, 2656
Cfr13I GGNCC 3 cut(s) 1825, 1826, 2115
CseI GACGC 1 cut(s) 2742
Csp6I GTAC 9 cut(s) 904, 1086, 1483, 1885, 1909, 1914, 1925, 2088, 2702
CspCI CAANNNNNGTGG 4 cut(s) 429, 464, 1668, 1703
CviAII CATG 8 cut(s) 813, 874, 928, 969, 1145, 1345, 1817, 2551
CviQI GTAC 9 cut(s) 904, 1086, 1483, 1885, 1909, 1914, 1925, 2088, 2702
DdeI CTNAG 8 cut(s) 140, 158, 246, 336, 1583, 1659, 2072, 2249
DpnI GATC 9 cut(s) 585, 591, 613, 1068, 1266, 1453, 1467, 1605, 2188
DpnII GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
EaeI YGGCCR 2 cut(s) 1554, 1748
Eam1104I CTCTTC 5 cut(s) 602, 1013, 1104, 1428, 1623
EarI CTCTTC 5 cut(s) 602, 1013, 1104, 1428, 1623
EciI GGCGGA 1 cut(s) 1323
Ecl136II GAGCTC 1 cut(s) 562
Eco130I CCWWGG 2 cut(s) 174, 627
Eco147I AGGCCT 1 cut(s) 1701
Eco24I GRGCYC 3 cut(s) 564, 1133, 1829
Eco32I GATATC 1 cut(s) 1580
Eco47I GGWCC 1 cut(s) 2115
Eco53kI GAGCTC 1 cut(s) 562
Eco57I CTGAAG 5 cut(s) 401, 636, 1550, 1924, 1994
Eco72I CACGTG 1 cut(s) 1442
Eco88I CYCGRG 2 cut(s) 126, 557
EcoICRI GAGCTC 1 cut(s) 562
EcoO109I RGGNCCY 1 cut(s) 2115
EcoRV GATATC 1 cut(s) 1580
EcoT14I CCWWGG 2 cut(s) 174, 627
EcoT38I GRGCYC 3 cut(s) 564, 1133, 1829
ErhI CCWWGG 2 cut(s) 174, 627
FaeI CATG 8 cut(s) 816, 877, 931, 972, 1148, 1348, 1820, 2554
FalI AAGNNNNNCTT 2 cut(s) 1826, 1858
FaqI GGGAC 1 cut(s) 2128
FatI CATG 8 cut(s) 812, 873, 927, 968, 1144, 1344, 1816, 2550
FauNDI CATATG 2 cut(s) 510, 1511
FbaI TGATCA 2 cut(s) 1264, 1465
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 8 cut(s) 188, 689, 1390, 1571, 2234, 2433, 2682, 2685
FokI GGATG 5 cut(s) 117, 644, 1903, 2518, 2533
FriOI GRGCYC 3 cut(s) 564, 1133, 1829
Fsp4HI GCNGC 8 cut(s) 188, 689, 1390, 1571, 2234, 2433, 2682, 2685
FspBI CTAG 7 cut(s) 950, 1089, 1194, 1338, 2222, 2541, 2792
GluI GCNGC 8 cut(s) 188, 689, 1390, 1571, 2234, 2433, 2682, 2685
GsaI CCCAGC 3 cut(s) 762, 1547, 1990
GsuI CTGGAG 2 cut(s) 977, 2678
HaeIII GGCC 5 cut(s) 626, 1556, 1701, 1750, 1827
HapII CCGG 5 cut(s) 1727, 2244, 2281, 2315, 2508
HgaI GACGC 1 cut(s) 2742
Hin1II CATG 8 cut(s) 816, 877, 931, 972, 1148, 1348, 1820, 2554
HincII GTYRAC 1 cut(s) 1447
HindII GTYRAC 1 cut(s) 1447
HindIII AAGCTT 2 cut(s) 938, 2353
HpaII CCGG 5 cut(s) 1727, 2244, 2281, 2315, 2508
HphI GGTGA 4 cut(s) 255, 1634, 2239, 2594
Hpy166II GTNNAC 4 cut(s) 670, 1297, 1447, 2480
Hpy8I GTNNAC 4 cut(s) 670, 1297, 1447, 2480
HpyCH4III ACNGT 7 cut(s) 317, 541, 1487, 1526, 1654, 1913, 2087
HpyCH4IV ACGT 7 cut(s) 110, 1299, 1441, 1686, 1887, 1896, 2744
HpyF10VI GCNNNNNNNGC 2 cut(s) 1553, 2491
HpyF3I CTNAG 8 cut(s) 140, 158, 246, 336, 1583, 1659, 2072, 2249
HpySE526I ACGT 7 cut(s) 110, 1299, 1441, 1686, 1887, 1896, 2744
Hsp92II CATG 8 cut(s) 816, 877, 931, 972, 1148, 1348, 1820, 2554
KflI GGGWCCC 1 cut(s) 2115
Kpn2I TCCGGA 2 cut(s) 2280, 2507
Ksp22I TGATCA 2 cut(s) 1264, 1465
Kzo9I GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
LmnI GCTCC 4 cut(s) 2056, 2323, 2510, 2653
Lsp1109I GCAGC 6 cut(s) 174, 700, 1582, 2444, 2668, 2671
LweI GCATC 6 cut(s) 95, 415, 1207, 2173, 2419, 2773
MaeI CTAG 7 cut(s) 950, 1089, 1194, 1338, 2222, 2541, 2792
MaeII ACGT 7 cut(s) 110, 1299, 1441, 1686, 1887, 1896, 2744
MaeIII GTNAC 3 cut(s) 45, 1752, 2794
MalI GATC 9 cut(s) 585, 591, 613, 1068, 1266, 1453, 1467, 1605, 2188
MboI GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
MfeI CAATTG 2 cut(s) 735, 1848
MflI RGATCY 2 cut(s) 589, 611
MhlI GDGCHC 5 cut(s) 564, 1133, 1593, 1829, 1978
MlsI TGGCCA 1 cut(s) 1556
MluNI TGGCCA 1 cut(s) 1556
MmeI TCCRAC 6 cut(s) 93, 112, 216, 727, 1396, 2193
Mox20I TGGCCA 1 cut(s) 1556
MroI TCCGGA 2 cut(s) 2280, 2507
MroXI GAANNNNTTC 3 cut(s) 681, 1055, 2067
MscI TGGCCA 1 cut(s) 1556
MslI CAYNNNNRTG 1 cut(s) 1149
Msp20I TGGCCA 1 cut(s) 1556
MspA1I CMGCKG 1 cut(s) 691
MspCI CTTAAG 1 cut(s) 1712
MspI CCGG 5 cut(s) 1727, 2244, 2281, 2315, 2508
MspR9I CCNGG 3 cut(s) 1728, 2244, 2316
MunI CAATTG 2 cut(s) 735, 1848
Mva1269I GAATGC 3 cut(s) 367, 1363, 2236
MvnI CGCG 2 cut(s) 1602, 2362
MwoI GCNNNNNNNGC 2 cut(s) 1553, 2491
NciI CCSGG 3 cut(s) 1728, 2244, 2316
NdeI CATATG 2 cut(s) 510, 1511
NdeII GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
NlaIII CATG 8 cut(s) 816, 877, 931, 972, 1148, 1348, 1820, 2554
NlaIV GGNNCC 5 cut(s) 1656, 1827, 1977, 2116, 2117
NmuCI GTSAC 1 cut(s) 45
NruI TCGCGA 1 cut(s) 1602
NspI RCATGY 1 cut(s) 931
NspV TTCGAA 1 cut(s) 834
PaeR7I CTCGAG 2 cut(s) 126, 557
PceI AGGCCT 1 cut(s) 1701
PciI ACATGT 1 cut(s) 927
PcsI WCGNNNNNNNCGW 1 cut(s) 1893
PctI GAATGC 3 cut(s) 367, 1363, 2236
PdmI GAANNNNTTC 3 cut(s) 681, 1055, 2067
PfeI GAWTC 2 cut(s) 1092, 1859
PfoI TCCNGGA 2 cut(s) 1726, 2242
PkrI GCNGC 8 cut(s) 189, 690, 1391, 1572, 2235, 2434, 2683, 2686
PmaCI CACGTG 1 cut(s) 1442
PmlI CACGTG 1 cut(s) 1442
Ppu21I YACGTR 3 cut(s) 1442, 1888, 2745
PpuMI RGGWCCY 1 cut(s) 2115
PscI ACATGT 1 cut(s) 927
PshAI GACNNNNGTC 1 cut(s) 1442
PshBI ATTAAT 1 cut(s) 1425
PsiI TTATAA 2 cut(s) 1031, 1785
Psp124BI GAGCTC 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 2115
PspCI CACGTG 1 cut(s) 1442
PspFI CCCAGC 3 cut(s) 758, 1543, 1986
PspN4I GGNNCC 5 cut(s) 1656, 1827, 1977, 2116, 2117
PspOMI GGGCCC 1 cut(s) 1825
PspPI GGNCC 3 cut(s) 1825, 1826, 2115
PspPPI RGGWCCY 1 cut(s) 2115
PspXI VCTCGAGB 2 cut(s) 126, 557
PstNI CAGNNNCTG 2 cut(s) 428, 2656
PsuI RGATCY 2 cut(s) 589, 611
PvuII CAGCTG 1 cut(s) 691
RruI TCGCGA 1 cut(s) 1602
RsaI GTAC 9 cut(s) 905, 1087, 1484, 1886, 1910, 1915, 1926, 2089, 2703
RsaNI GTAC 9 cut(s) 904, 1086, 1483, 1885, 1909, 1914, 1925, 2088, 2702
RseI CAYNNNNRTG 1 cut(s) 1149
SacI GAGCTC 1 cut(s) 564
SatI GCNGC 8 cut(s) 188, 689, 1390, 1571, 2234, 2433, 2682, 2685
Sau3AI GATC 9 cut(s) 583, 589, 611, 1066, 1264, 1451, 1465, 1603, 2186
Sau96I GGNCC 3 cut(s) 1825, 1826, 2115
ScaI AGTACT 2 cut(s) 1087, 2089
ScrFI CCNGG 3 cut(s) 1728, 2244, 2316
SduI GDGCHC 5 cut(s) 564, 1133, 1593, 1829, 1978
SfaNI GCATC 6 cut(s) 95, 415, 1207, 2173, 2419, 2773
SfcI CTRYAG 1 cut(s) 2146
Sfr274I CTCGAG 2 cut(s) 126, 557
SfuI TTCGAA 1 cut(s) 834
SinI GGWCC 1 cut(s) 2115
SlaI CTCGAG 2 cut(s) 126, 557
SmiMI CAYNNNNRTG 1 cut(s) 1149
SmlI CTYRAG 9 cut(s) 11, 126, 301, 557, 772, 851, 1504, 1712, 2689
SmoI CTYRAG 9 cut(s) 11, 126, 301, 557, 772, 851, 1504, 1712, 2689
SpeI ACTAGT 1 cut(s) 2540
SseBI AGGCCT 1 cut(s) 1701
SsiI CCGC 6 cut(s) 220, 686, 1334, 1389, 2233, 2560
SspI AATATT 5 cut(s) 718, 2026, 2067, 2387, 2398
SspMI CTAG 7 cut(s) 950, 1089, 1194, 1338, 2222, 2541, 2792
SstI GAGCTC 1 cut(s) 564
StuI AGGCCT 1 cut(s) 1701
StyD4I CCNGG 3 cut(s) 1726, 2242, 2314
StyI CCWWGG 2 cut(s) 174, 627
TaaI ACNGT 7 cut(s) 317, 541, 1487, 1526, 1654, 1913, 2087
TaiI ACGT 7 cut(s) 113, 1302, 1444, 1689, 1890, 1899, 2747
TaqI TCGA 6 cut(s) 127, 294, 558, 834, 1758, 2750
TatI WGTACW 5 cut(s) 903, 1085, 1482, 2087, 2701
TauI GCSGC 2 cut(s) 1392, 2236
TfiI GAWTC 2 cut(s) 1092, 1859
TscAI CASTG 7 cut(s) 1169, 1475, 1836, 2080, 2196, 2349, 2730
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 6 cut(s) 187, 688, 1570, 2432, 2681, 2684
Tsp45I GTSAC 1 cut(s) 45
TspDTI ATGAA 8 cut(s) 323, 620, 924, 935, 1048, 1851, 2390, 2535
TspGWI ACGGA 2 cut(s) 2436, 2639
TspRI CASTG 7 cut(s) 1169, 1475, 1836, 2080, 2196, 2349, 2730
Vha464I CTTAAG 1 cut(s) 1712
VpaK11BI GGWCC 1 cut(s) 2115
VspI ATTAAT 1 cut(s) 1425
XapI RAATTY 8 cut(s) 521, 981, 1004, 1499, 1902, 2133, 2254, 2284
XbaI TCTAGA 2 cut(s) 949, 2221
XceI RCATGY 1 cut(s) 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XhoI CTCGAG 2 cut(s) 126, 557
XmiI GTMKAC 1 cut(s) 669
XmnI GAANNNNTTC 3 cut(s) 681, 1055, 2067
XspI CTAG 7 cut(s) 950, 1089, 1194, 1338, 2222, 2541, 2792
ZrmI AGTACT 2 cut(s) 1087, 2089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.