FvH4_3g10392

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000093
Physical Location & Seq
Forward (+)
6091499 .. 6092107
609 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10392.t1

Sequence Viewer

Length: 609 bp
ATGATAATTGACTGCAAATCAATTAGAGAGGAGTTTCTCACCAAGGTTGTTTTTCTGCTTTGGGAAATTTGGAAAGCTAGGAATGCTTTTGTCTTTGAGGCTACTCCAATTGATCCAAGAATGGTGGCCAAGAAAGCTGGTACTGCTGCTGCAGAATTCATCACCTGCAAAAACCACAATCTCATGTCAAGATCAATTTCTCTGTCTCATATGCACTCCAGCCAGACGCAGTCGGGGAGTAATTTGACCCAAAGTTGGAGGCCCCCTCCTCCTGGTCATATTAAAATCAACTGTGATGCAGCATGGATTGAAAGTTCGAAGCTTACCGGCATTTGTGCTATAGCTCGTGATTCTCATGGCTCCCCCTTTGATGGAGCTACCTTGTTGTGCCGAACGGGATCTGTGTTAGAGGCGGAGGCGACGGCGGCGGCGGTGGCTATCGATGTGGCCAAGCGCATCCCTTCTTCTCCAATTATCCTCGAGTCAGATTCAAAGACATTAGTGGACAACATCAACATTTCAAGCAGTACATGTGATTGGAGAATTGCTCCAATTGTGTCTATGATTAGAAATTCAATTACCTCGAACCCATGTTTGGCGTGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

203

Amino Acids

22.06

Weight (kDa)

8.19

Isoelectric Point (pI)

56.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 97 - 194 2.8e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 173
Acc36I ACCTGC 1 cut(s) 173
AciI CCGC 4 cut(s) 413, 425, 428, 431
AclWI GGATC 2 cut(s) 107, 406
AcoI YGGCCR 2 cut(s) 126, 447
AcsI RAATTY 3 cut(s) 66, 155, 571
AfaI GTAC 2 cut(s) 142, 529
AfiI CCNNNNNNNGG 4 cut(s) 255, 272, 371, 595
AflIII ACRYGT 1 cut(s) 530
AgsI TTSAA 4 cut(s) 311, 492, 522, 576
AjnI CCWGG 1 cut(s) 271
AjuI GAANNNNNNNTTGG 1 cut(s) 578
AloI GAACNNNNNNTCC 2 cut(s) 298, 330
AluBI AGCT 5 cut(s) 77, 137, 322, 344, 377
AluI AGCT 5 cut(s) 77, 137, 322, 344, 377
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 2 cut(s) 107, 406
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 3 cut(s) 126, 260, 447
ApeKI GCWGC 3 cut(s) 146, 149, 299
ApoI RAATTY 3 cut(s) 66, 155, 571
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 2 cut(s) 31, 154
AsuII TTCGAA 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 479
BalI TGGCCA 2 cut(s) 128, 449
BauI CACGAG 1 cut(s) 345
BbvI GCAGC 3 cut(s) 133, 136, 311
BccI CCATC 1 cut(s) 365
BceAI ACGGC 1 cut(s) 438
BciT130I CCWGG 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 2 cut(s) 78, 607
BfmI CTRYAG 2 cut(s) 150, 339
BfuAI ACCTGC 1 cut(s) 173
BisI GCNGC 5 cut(s) 147, 150, 300, 426, 429
BlsI GCNGC 5 cut(s) 148, 151, 301, 427, 430
Bme1390I CCNGG 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 263, 361
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 2 cut(s) 286, 465
BplI GAGNNNNNCTC 4 cut(s) 250, 282, 532, 564
BpmI CTGGAG 1 cut(s) 202
Bpu14I TTCGAA 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 42
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 272, 371, 595
Bse118I RCCGGY 1 cut(s) 326
BseBI CCWGG 1 cut(s) 273
BseCI ATCGAT 1 cut(s) 441
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 4 cut(s) 255, 272, 371, 595
BseRI GAGGAG 2 cut(s) 44, 258
BseXI GCAGC 3 cut(s) 133, 136, 311
BshFI GGCC 3 cut(s) 128, 262, 449
BshVI ATCGAT 1 cut(s) 441
BsiHKCI CYCGRG 1 cut(s) 479
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 4 cut(s) 255, 272, 371, 595
BsmAI GTCTC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 3 cut(s) 128, 262, 449
BsoBI CYCGRG 1 cut(s) 479
Bsp119I TTCGAA 1 cut(s) 317
Bsp143I GATC 3 cut(s) 112, 191, 398
BspACI CCGC 4 cut(s) 413, 425, 428, 431
BspANI GGCC 3 cut(s) 128, 262, 449
BspDI ATCGAT 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 263, 361
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 173
BspPI GGATC 2 cut(s) 107, 406
BspT104I TTCGAA 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 326
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 3 cut(s) 112, 191, 398
BssSI CACGAG 1 cut(s) 345
BssT1I CCWWGG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 317
BstF5I GGATG 1 cut(s) 456
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 3 cut(s) 115, 194, 401
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 3 cut(s) 112, 191, 398
BstMWI GCNNNNNNNGC 5 cut(s) 83, 134, 143, 425, 434
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 1 cut(s) 534
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 2 cut(s) 150, 339
BstV1I GCAGC 3 cut(s) 133, 136, 311
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 441
BsuRI GGCC 3 cut(s) 128, 262, 449
BsuTUI ATCGAT 1 cut(s) 441
BtsCI GGATG 1 cut(s) 456
BveI ACCTGC 1 cut(s) 173
CfoI GCGC 1 cut(s) 456
Cfr10I RCCGGY 1 cut(s) 326
Cfr13I GGNCC 1 cut(s) 261
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 1 cut(s) 235
Csp6I GTAC 2 cut(s) 141, 528
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 5 cut(s) 184, 303, 356, 531, 591
CviQI GTAC 2 cut(s) 141, 528
DpnI GATC 3 cut(s) 114, 193, 400
DpnII GATC 3 cut(s) 112, 191, 398
EaeI YGGCCR 2 cut(s) 126, 447
EciI GGCGGA 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 479
EcoO109I RGGNCCY 1 cut(s) 261
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 5 cut(s) 187, 306, 359, 534, 594
FatI CATG 5 cut(s) 183, 302, 355, 530, 590
FauNDI CATATG 1 cut(s) 210
Fnu4HI GCNGC 5 cut(s) 147, 150, 300, 426, 429
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 5 cut(s) 147, 150, 300, 426, 429
FspBI CTAG 2 cut(s) 78, 607
GlaI GCGC 1 cut(s) 455
GluI GCNGC 5 cut(s) 147, 150, 300, 426, 429
GsuI CTGGAG 1 cut(s) 202
HaeIII GGCC 3 cut(s) 128, 262, 449
HapII CCGG 1 cut(s) 327
HgaI GACGC 1 cut(s) 235
HhaI GCGC 1 cut(s) 456
Hin1II CATG 5 cut(s) 187, 306, 359, 534, 594
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 3 cut(s) 350, 482, 488
HpaII CCGG 1 cut(s) 327
HphI GGTGA 2 cut(s) 31, 154
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 1 cut(s) 487
Hpy188III TCNNGA 2 cut(s) 189, 347
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 424
HpyAV CCTTC 1 cut(s) 471
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4V TGCA 5 cut(s) 15, 152, 168, 214, 299
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 134, 143, 425, 434
Hsp92II CATG 5 cut(s) 187, 306, 359, 534, 594
HspAI GCGC 1 cut(s) 454
Kzo9I GATC 3 cut(s) 112, 191, 398
LmnI GCTCC 3 cut(s) 365, 374, 553
LpnPI CCDG 7 cut(s) 123, 178, 232, 236, 258, 285, 340
Lsp1109I GCAGC 3 cut(s) 133, 136, 311
LweI GCATC 2 cut(s) 286, 465
MaeI CTAG 2 cut(s) 78, 607
MalI GATC 3 cut(s) 114, 193, 400
MboI GATC 3 cut(s) 112, 191, 398
MboII GAAGA 1 cut(s) 456
MfeI CAATTG 2 cut(s) 108, 552
MflI RGATCY 1 cut(s) 398
MlsI TGGCCA 2 cut(s) 128, 449
MluNI TGGCCA 2 cut(s) 128, 449
MlyI GAGTC 1 cut(s) 491
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 9 cut(s) 22, 91, 252, 276, 279, 403, 409, 488, 592
Mox20I TGGCCA 2 cut(s) 128, 449
MscI TGGCCA 2 cut(s) 128, 449
MseI TTAA 1 cut(s) 282
Msp20I TGGCCA 2 cut(s) 128, 449
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 273
MunI CAATTG 2 cut(s) 108, 552
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 5 cut(s) 83, 134, 143, 425, 434
NdeI CATATG 1 cut(s) 210
NdeII GATC 3 cut(s) 112, 191, 398
NlaIII CATG 5 cut(s) 187, 306, 359, 534, 594
NlaIV GGNNCC 2 cut(s) 263, 361
NspI RCATGY 1 cut(s) 534
NspV TTCGAA 1 cut(s) 317
PaeR7I CTCGAG 1 cut(s) 479
PaqCI CACCTGC 1 cut(s) 173
PciI ACATGT 1 cut(s) 530
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 2 cut(s) 350, 488
PflFI GACNNNGTC 1 cut(s) 229
PkrI GCNGC 5 cut(s) 148, 151, 301, 427, 430
PleI GAGTC 1 cut(s) 490
PpsI GAGTC 1 cut(s) 490
PscI ACATGT 1 cut(s) 530
Psp6I CCWGG 1 cut(s) 271
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 2 cut(s) 263, 361
PspPI GGNCC 1 cut(s) 261
PspXI VCTCGAGB 1 cut(s) 479
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 398
PsyI GACNNNGTC 1 cut(s) 229
RsaI GTAC 2 cut(s) 142, 529
RsaNI GTAC 2 cut(s) 141, 528
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 5 cut(s) 147, 150, 300, 426, 429
Sau3AI GATC 3 cut(s) 112, 191, 398
Sau96I GGNCC 1 cut(s) 261
SchI GAGTC 1 cut(s) 491
ScrFI CCNGG 1 cut(s) 273
SetI ASST 9 cut(s) 48, 79, 139, 167, 324, 346, 379, 383, 584
SfaNI GCATC 2 cut(s) 286, 465
SfcI CTRYAG 2 cut(s) 150, 339
Sfr274I CTCGAG 1 cut(s) 479
SfuI TTCGAA 1 cut(s) 317
SlaI CTCGAG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SsiI CCGC 4 cut(s) 413, 425, 428, 431
SspMI CTAG 2 cut(s) 78, 607
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 293
TaqI TCGA 4 cut(s) 317, 441, 480, 584
TatI WGTACW 1 cut(s) 527
TauI GCSGC 2 cut(s) 428, 431
TfiI GAWTC 2 cut(s) 350, 488
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseI GCWGC 3 cut(s) 146, 149, 299
TspDTI ATGAA 1 cut(s) 148
Tth111I GACNNNGTC 1 cut(s) 229
XapI RAATTY 3 cut(s) 66, 155, 571
XceI RCATGY 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 597
XhoI CTCGAG 1 cut(s) 479
XspI CTAG 2 cut(s) 78, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.