Ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 366 |
| AccB1I | GGYRCC | 1 cut(s) | 585 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 351 |
| AccI | GTMKAC | 1 cut(s) | 185 |
| AcvI | CACGTG | 1 cut(s) | 260 |
| AcyI | GRCGYC | 1 cut(s) | 586 |
| AfaI | GTAC | 2 cut(s) | 988, 1038 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 351 |
| AgsI | TTSAA | 4 cut(s) | 76, 466, 508, 673 |
| AjnI | CCWGG | 2 cut(s) | 157, 699 |
| AluBI | AGCT | 7 cut(s) | 331, 407, 421, 571, 842, 856, 1007 |
| AluI | AGCT | 7 cut(s) | 331, 407, 421, 571, 842, 856, 1007 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 117 |
| AoxI | GGCC | 1 cut(s) | 78 |
| ApeKI | GCWGC | 3 cut(s) | 16, 407, 1109 |
| AspLEI | GCGC | 1 cut(s) | 588 |
| AspS9I | GGNCC | 1 cut(s) | 872 |
| AsuHPI | GGTGA | 2 cut(s) | 898, 1078 |
| AvaII | GGWCC | 1 cut(s) | 872 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 783, 816 |
| BanI | GGYRCC | 1 cut(s) | 585 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 113, 145 |
| BbrPI | CACGTG | 1 cut(s) | 260 |
| BbsI | GAAGAC | 1 cut(s) | 384 |
| BbvI | GCAGC | 3 cut(s) | 28, 394, 1096 |
| BccI | CCATC | 3 cut(s) | 576, 617, 804 |
| BcgI | CGANNNNNNTGC | 5 cut(s) | 29, 536, 570, 1091, 1125 |
| BciT130I | CCWGG | 2 cut(s) | 159, 701 |
| BclI | TGATCA | 1 cut(s) | 844 |
| BfaI | CTAG | 3 cut(s) | 309, 332, 770 |
| BfmI | CTRYAG | 2 cut(s) | 687, 837 |
| BfoI | RGCGCY | 1 cut(s) | 589 |
| BisI | GCNGC | 3 cut(s) | 17, 408, 1110 |
| BlsI | GCNGC | 3 cut(s) | 18, 409, 1111 |
| BmcAI | AGTACT | 1 cut(s) | 1038 |
| Bme1390I | CCNGG | 2 cut(s) | 159, 701 |
| Bme18I | GGWCC | 1 cut(s) | 872 |
| BmgT120I | GGNCC | 1 cut(s) | 872 |
| BmiI | GGNNCC | 1 cut(s) | 587 |
| BmrFI | CCNGG | 2 cut(s) | 159, 701 |
| BmsI | GCATC | 3 cut(s) | 28, 331, 1098 |
| BpiI | GAAGAC | 1 cut(s) | 384 |
| BpmI | CTGGAG | 2 cut(s) | 437, 683 |
| Bpu10I | CCTNAGC | 3 cut(s) | 253, 393, 916 |
| BpuEI | CTTGAG | 1 cut(s) | 617 |
| BsaAI | YACGTR | 1 cut(s) | 260 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1014 |
| BsaHI | GRCGYC | 1 cut(s) | 586 |
| BsaJI | CCNNGG | 2 cut(s) | 45, 1062 |
| BsaWI | WCCGGW | 1 cut(s) | 763 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 351 |
| Bse3DI | GCAATG | 1 cut(s) | 913 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1014 |
| BseBI | CCWGG | 2 cut(s) | 159, 701 |
| BseDI | CCNNGG | 2 cut(s) | 45, 1062 |
| BseGI | GGATG | 3 cut(s) | 41, 587, 717 |
| BseJI | GATNNNNATC | 1 cut(s) | 1014 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 351 |
| BseMI | GCAATG | 1 cut(s) | 913 |
| BseMII | CTCAG | 2 cut(s) | 407, 930 |
| BseRI | GAGGAG | 2 cut(s) | 62, 866 |
| BseXI | GCAGC | 3 cut(s) | 28, 394, 1096 |
| BsgI | GTGCAG | 1 cut(s) | 35 |
| BshFI | GGCC | 1 cut(s) | 80 |
| BshNI | GGYRCC | 1 cut(s) | 585 |
| BsiSI | CCGG | 2 cut(s) | 764, 1144 |
| BslFI | GGGAC | 1 cut(s) | 640 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 351 |
| BsmFI | GGGAC | 1 cut(s) | 640 |
| BsmI | GAATGC | 4 cut(s) | 295, 712, 891, 1111 |
| BsnI | GGCC | 1 cut(s) | 80 |
| Bsp143I | GATC | 1 cut(s) | 844 |
| BspANI | GGCC | 1 cut(s) | 80 |
| BspCNI | CTCAG | 2 cut(s) | 406, 929 |
| BspHI | TCATGA | 3 cut(s) | 496, 847, 967 |
| BspLI | GGNNCC | 1 cut(s) | 587 |
| BspT107I | GGYRCC | 1 cut(s) | 585 |
| BsrDI | GCAATG | 1 cut(s) | 913 |
| BssECI | CCNNGG | 2 cut(s) | 45, 1062 |
| BssMI | GATC | 1 cut(s) | 844 |
| BssNI | GRCGYC | 1 cut(s) | 586 |
| BssT1I | CCWWGG | 1 cut(s) | 1062 |
| Bst2UI | CCWGG | 2 cut(s) | 159, 701 |
| Bst4CI | ACNGT | 7 cut(s) | 190, 359, 642, 688, 961, 991, 997 |
| BstACI | GRCGYC | 1 cut(s) | 586 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 248, 912 |
| BstBAI | YACGTR | 1 cut(s) | 260 |
| BstC8I | GCNNGC | 3 cut(s) | 109, 555, 913 |
| BstDEI | CTNAG | 4 cut(s) | 253, 393, 916, 1167 |
| BstF5I | GGATG | 3 cut(s) | 41, 587, 717 |
| BstH2I | RGCGCY | 1 cut(s) | 589 |
| BstHHI | GCGC | 1 cut(s) | 588 |
| BstKTI | GATC | 1 cut(s) | 847 |
| BstMBI | GATC | 1 cut(s) | 844 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 16, 248, 404, 912 |
| BstNI | CCWGG | 2 cut(s) | 159, 701 |
| BstSCI | CCNGG | 2 cut(s) | 157, 699 |
| BstSFI | CTRYAG | 2 cut(s) | 687, 837 |
| BstV1I | GCAGC | 3 cut(s) | 28, 394, 1096 |
| BstV2I | GAAGAC | 1 cut(s) | 384 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 810 |
| BsuRI | GGCC | 1 cut(s) | 80 |
| BtsCI | GGATG | 3 cut(s) | 41, 587, 717 |
| BtsIMutI | CAGTG | 1 cut(s) | 1002 |
| Cac8I | GCNNGC | 3 cut(s) | 109, 555, 913 |
| CaiI | CAGNNNCTG | 1 cut(s) | 117 |
| CciI | TCATGA | 3 cut(s) | 496, 847, 967 |
| CfoI | GCGC | 1 cut(s) | 588 |
| Cfr13I | GGNCC | 1 cut(s) | 872 |
| CseI | GACGC | 2 cut(s) | 282, 767 |
| Csp6I | GTAC | 2 cut(s) | 987, 1037 |
| CviAII | CATG | 3 cut(s) | 497, 848, 968 |
| CviQI | GTAC | 2 cut(s) | 987, 1037 |
| DdeI | CTNAG | 4 cut(s) | 253, 393, 916, 1167 |
| DinI | GGCGCC | 1 cut(s) | 587 |
| DpnI | GATC | 1 cut(s) | 846 |
| DpnII | GATC | 1 cut(s) | 844 |
| Eco130I | CCWWGG | 1 cut(s) | 1062 |
| Eco147I | AGGCCT | 1 cut(s) | 80 |
| Eco47I | GGWCC | 1 cut(s) | 872 |
| Eco72I | CACGTG | 1 cut(s) | 260 |
| EcoRII | CCWGG | 2 cut(s) | 157, 699 |
| EcoT14I | CCWWGG | 1 cut(s) | 1062 |
| EgeI | GGCGCC | 1 cut(s) | 587 |
| EheI | GGCGCC | 1 cut(s) | 587 |
| ErhI | CCWWGG | 1 cut(s) | 1062 |
| FaeI | CATG | 3 cut(s) | 500, 851, 971 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 473, 505, 895, 927 |
| FaqI | GGGAC | 1 cut(s) | 640 |
| FatI | CATG | 3 cut(s) | 496, 847, 967 |
| FbaI | TGATCA | 1 cut(s) | 844 |
| FblI | GTMKAC | 1 cut(s) | 185 |
| Fnu4HI | GCNGC | 3 cut(s) | 17, 408, 1110 |
| FokI | GGATG | 3 cut(s) | 28, 594, 704 |
| Fsp4HI | GCNGC | 3 cut(s) | 17, 408, 1110 |
| FspBI | CTAG | 3 cut(s) | 309, 332, 770 |
| GlaI | GCGC | 1 cut(s) | 587 |
| GluI | GCNGC | 3 cut(s) | 17, 408, 1110 |
| GsuI | CTGGAG | 2 cut(s) | 437, 683 |
| HaeII | RGCGCY | 1 cut(s) | 589 |
| HaeIII | GGCC | 1 cut(s) | 80 |
| HapII | CCGG | 2 cut(s) | 764, 1144 |
| HgaI | GACGC | 2 cut(s) | 282, 767 |
| HhaI | GCGC | 1 cut(s) | 588 |
| Hin1I | GRCGYC | 1 cut(s) | 586 |
| Hin1II | CATG | 3 cut(s) | 500, 851, 971 |
| Hin6I | GCGC | 1 cut(s) | 586 |
| HinP1I | GCGC | 1 cut(s) | 586 |
| HincII | GTYRAC | 1 cut(s) | 297 |
| HindII | GTYRAC | 1 cut(s) | 297 |
| HindIII | AAGCTT | 1 cut(s) | 569 |
| HinfI | GANTC | 7 cut(s) | 162, 314, 452, 538, 673, 859, 929 |
| HpaII | CCGG | 2 cut(s) | 764, 1144 |
| HphI | GGTGA | 2 cut(s) | 898, 1078 |
| Hpy166II | GTNNAC | 2 cut(s) | 186, 297 |
| Hpy188I | TCNGA | 3 cut(s) | 46, 102, 194 |
| Hpy188III | TCNNGA | 7 cut(s) | 118, 497, 596, 782, 848, 968, 1102 |
| Hpy8I | GTNNAC | 2 cut(s) | 186, 297 |
| HpyAV | CCTTC | 2 cut(s) | 115, 475 |
| HpyCH4III | ACNGT | 7 cut(s) | 190, 359, 642, 688, 961, 991, 997 |
| HpyCH4IV | ACGT | 1 cut(s) | 259 |
| HpyCH4V | TGCA | 5 cut(s) | 16, 263, 710, 1089, 1164 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 16, 248, 404, 912 |
| HpyF3I | CTNAG | 4 cut(s) | 253, 393, 916, 1167 |
| HpySE526I | ACGT | 1 cut(s) | 259 |
| Hsp92I | GRCGYC | 1 cut(s) | 586 |
| Hsp92II | CATG | 3 cut(s) | 500, 851, 971 |
| HspAI | GCGC | 1 cut(s) | 586 |
| KasI | GGCGCC | 1 cut(s) | 585 |
| Ksp22I | TGATCA | 1 cut(s) | 844 |
| Kzo9I | GATC | 1 cut(s) | 844 |
| LmnI | GCTCC | 2 cut(s) | 418, 853 |
| Lsp1109I | GCAGC | 3 cut(s) | 28, 394, 1096 |
| LweI | GCATC | 3 cut(s) | 28, 331, 1098 |
| MaeI | CTAG | 3 cut(s) | 309, 332, 770 |
| MaeII | ACGT | 1 cut(s) | 259 |
| MaeIII | GTNAC | 3 cut(s) | 311, 991, 1066 |
| MalI | GATC | 1 cut(s) | 846 |
| MboI | GATC | 1 cut(s) | 844 |
| MboII | GAAGA | 2 cut(s) | 337, 389 |
| MfeI | CAATTG | 1 cut(s) | 243 |
| MluCI | AATT | 5 cut(s) | 177, 243, 723, 829, 963 |
| Mly113I | GGCGCC | 1 cut(s) | 586 |
| MlyI | GAGTC | 2 cut(s) | 308, 446 |
| MnlI | CCTC | 6 cut(s) | 40, 86, 165, 592, 603, 844 |
| MseI | TTAA | 5 cut(s) | 149, 444, 491, 525, 563 |
| MspA1I | CMGCKG | 1 cut(s) | 407 |
| MspI | CCGG | 2 cut(s) | 764, 1144 |
| MspR9I | CCNGG | 2 cut(s) | 159, 701 |
| MunI | CAATTG | 1 cut(s) | 243 |
| Mva1269I | GAATGC | 4 cut(s) | 295, 712, 891, 1111 |
| MvaI | CCWGG | 2 cut(s) | 159, 701 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 16, 248, 404, 912 |
| NarI | GGCGCC | 1 cut(s) | 586 |
| NdeII | GATC | 1 cut(s) | 844 |
| NlaIII | CATG | 3 cut(s) | 500, 851, 971 |
| NlaIV | GGNNCC | 1 cut(s) | 587 |
| NmuCI | GTSAC | 2 cut(s) | 311, 1066 |
| PagI | TCATGA | 3 cut(s) | 496, 847, 967 |
| PceI | AGGCCT | 1 cut(s) | 80 |
| PctI | GAATGC | 4 cut(s) | 295, 712, 891, 1111 |
| PfeI | GAWTC | 5 cut(s) | 162, 538, 673, 859, 929 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 351 |
| PfoI | TCCNGGA | 2 cut(s) | 157, 699 |
| PkrI | GCNGC | 3 cut(s) | 18, 409, 1111 |
| PleI | GAGTC | 2 cut(s) | 308, 446 |
| PluTI | GGCGCC | 1 cut(s) | 589 |
| PmaCI | CACGTG | 1 cut(s) | 260 |
| PmlI | CACGTG | 1 cut(s) | 260 |
| PpsI | GAGTC | 2 cut(s) | 308, 446 |
| Ppu21I | YACGTR | 1 cut(s) | 260 |
| PsiI | TTATAA | 1 cut(s) | 366 |
| Psp6I | CCWGG | 2 cut(s) | 157, 699 |
| PspCI | CACGTG | 1 cut(s) | 260 |
| PspGI | CCWGG | 2 cut(s) | 157, 699 |
| PspN4I | GGNNCC | 1 cut(s) | 587 |
| PspPI | GGNCC | 1 cut(s) | 872 |
| PstNI | CAGNNNCTG | 1 cut(s) | 117 |
| PvuII | CAGCTG | 1 cut(s) | 407 |
| RsaI | GTAC | 2 cut(s) | 988, 1038 |
| RsaNI | GTAC | 2 cut(s) | 987, 1037 |
| SaqAI | TTAA | 5 cut(s) | 149, 444, 491, 525, 563 |
| SatI | GCNGC | 3 cut(s) | 17, 408, 1110 |
| Sau3AI | GATC | 1 cut(s) | 844 |
| Sau96I | GGNCC | 1 cut(s) | 872 |
| ScaI | AGTACT | 1 cut(s) | 1038 |
| SchI | GAGTC | 2 cut(s) | 308, 446 |
| ScrFI | CCNGG | 2 cut(s) | 159, 701 |
| SfaNI | GCATC | 3 cut(s) | 28, 331, 1098 |
| SfcI | CTRYAG | 2 cut(s) | 687, 837 |
| SfoI | GGCGCC | 1 cut(s) | 587 |
| SinI | GGWCC | 1 cut(s) | 872 |
| SmlI | CTYRAG | 1 cut(s) | 596 |
| SmoI | CTYRAG | 1 cut(s) | 596 |
| Sse9I | AATT | 5 cut(s) | 177, 243, 723, 829, 963 |
| SseBI | AGGCCT | 1 cut(s) | 80 |
| SspDI | GGCGCC | 1 cut(s) | 585 |
| SspMI | CTAG | 3 cut(s) | 309, 332, 770 |
| StuI | AGGCCT | 1 cut(s) | 80 |
| StyD4I | CCNGG | 2 cut(s) | 157, 699 |
| StyI | CCWWGG | 1 cut(s) | 1062 |
| TaaI | ACNGT | 7 cut(s) | 190, 359, 642, 688, 961, 991, 997 |
| TaiI | ACGT | 1 cut(s) | 262 |
| TaqI | TCGA | 2 cut(s) | 450, 1101 |
| TasI | AATT | 5 cut(s) | 177, 243, 723, 829, 963 |
| TatI | WGTACW | 2 cut(s) | 986, 1036 |
| TfiI | GAWTC | 5 cut(s) | 162, 538, 673, 859, 929 |
| Tru1I | TTAA | 5 cut(s) | 149, 444, 491, 525, 563 |
| Tru9I | TTAA | 5 cut(s) | 149, 444, 491, 525, 563 |
| TscAI | CASTG | 1 cut(s) | 1002 |
| TseFI | GTSAC | 2 cut(s) | 311, 1066 |
| TseI | GCWGC | 3 cut(s) | 16, 407, 1109 |
| Tsp45I | GTSAC | 2 cut(s) | 311, 1066 |
| TspDTI | ATGAA | 4 cut(s) | 390, 422, 743, 956 |
| TspRI | CASTG | 1 cut(s) | 1002 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 351 |
| VpaK11BI | GGWCC | 1 cut(s) | 872 |
| XmiI | GTMKAC | 1 cut(s) | 185 |
| XspI | CTAG | 3 cut(s) | 309, 332, 770 |
| ZrmI | AGTACT | 1 cut(s) | 1038 |