Rorug05G0192100

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000093
Physical Location & Seq
Reverse (-)
18837034 .. 18841095
4062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0192100.1

Sequence Viewer

Length: 4062 bp
ATGATCCATATTCACAAGCCGGTAATTGTATGCCTCTCTGAACCTTTTACCTTAATTCATTCAATTCCTTTTTCCTTTTGGAGATCCATAGGACTTATTCCTGTGGCTACTAATGATCGGGGCTTACAAGACCCCAACCTTTGGTTTCTTTGTCACAGGGACCTTGACCCTATAGTAATCTCCTCTTCTGCTCAACAAATCACTGTTTCCGTCATCCTCGACGGTGTTCAGTGTTTCATGACAGCAGTGTATGCCAAAACAACCATTGCAGGTCGTCGTCAACTTTGGCAAGACCTCTACCATGTTCACACTAACCTAGTGGTTGGTCCGTGGATTGTCTTCGGTGATTTTAACTGTGTTATGGGAGCCCACGAAAAAAGAGGTGGAAATCCTCTCAGCGCTGCCTCGTGTTTTGATTTCCAGCAGATGTGCTCAAACTGCCATCTTCTTGATGTTCATACTAGGGGCCTGTCTTACACGTGGACTAATAGGAGAACCTTTGAAAGGCTTGATAGGGCCCTTTGTAATTCAAGATGGTTGGATGCATGGAACAACTTTGATTGTCGGACATTAACCCGTGCGGCTTCTGATCATTGTCCTTTGTTAATGAGCTGCTCTCGTCTGCCATTTGTTTCTAAGCCTCAGTTCAAATTCCAAAATATGTGGCTGCAACACCCGGAATTCCATTGTACGGTCAGTAATTTCTGGGCTACTCTTCATTTTGTGGGTTGCCCGATGTTTGTGCTTTCCTCAAAGCTCCGGGCGCTGAAGAATATGCTCAAAGGTTGGAATGTCAATGTTTTTGGGAATGTTTATCAATCTGTTAATTCCTGTGAGGCGGCTCTAAACCTGGTCCAGCATGAAATTTCCAACTTGGGCCCTTCACCTGATCGGATCCAAAGAGAGGACCAGGCTCATTTGCAATTTCAACGAGCTTTGAAAATGCAGGACACTTTTCTTCGAGACAAATCTAGAATCAGATGGCTACGTGAGGGGGATAGGAACACATCTTTCCTTCATAATATGGTCAAAATCCGAAGGCTGCACACGTCATTGGCCTCCTTGCGGGCTGGTGGTACGCTTCTACAATCTCATGATTTGATTGCTGGTCATATTGTTAATTATTTTCAGATGGCTTTCACCAAGGATGGTAATCTTGAAGACTCTGGCCTGGTAGAGAGAGTAATCCCAAATCTGGTTACGCCCCTTGAAAACACTGCTCTTACTTCGTTGCCTTCTTCGGAGGATATTCTTGCCACTGTCCTTTCAATGGATGACTTTAGTGCGCCTGGGCCTGATGGATACGGCGGCTGTTTTTTCAAAAAATGTTGGAGTGTTGTTGCTCCAGATGTTATTTCTGCTGTCCAAAGCTTCTTCATTACTGGCTTCATCCCTCCCCACTTCAACTCAAATGTTATAATCTTGATTCCAAAGAAACCAGAAGCCGATTGTGCTACTGATTTCAGGCCTATTGCTCTTGCTAATTTTGCTTTCAAAATTATCACCAAGATTATGGCCACACGCCTCGCTCCAATTGCAGCCAGAATCATCTCCCCAAATCAAAGCGCTTTTATCACAGGGAGGTCTATTGCTGACCCTATTATTCTTACCTCTGAATGTATAAATTTGTTAGACAACCACTGCAAAACTGGTAACATTGCCTTAAAATTAGATATCAGTAAGGCCTTTGATACCATGGACTGGGAATTTCTGCTTAGGGTTCTAAAGTCTTTTGGTTTCGACCCTATTTTCATTAATTGGGTTCATAGCATCCTCAGATCTGCTTATCTTTCTGTGTTGGTGAATGGTCAGCATTGTGGGTTCTTCACTTGTTCTAGGGGTGTAAGGCAGGGTGACCCTTTGTCTCCTCTCTTATTTTGCATTGCAGAGGAGGTTCTCAGTCGTGGCTTGACTAATCTGGTTGATAGGGGAAAGCTTAGCCGGATTGCCTGTCCAGGTAAAGTAACTCCCCCCTCGCATGTTTTGTTTGCAGATGATGTTATGATTTTTATGCAAGGAAGCTCCCGTGGCATTCGTGCCTTAATGCGTTTCCTGGATGAATATGCCCTTAACTCTGGCCAAGTTATCAACAAAGCAAAATCTTCGGTTTTTCTGGGTAAACATGCTCGGACTAGAGAAATTATTATCCAACGTCTCCTAGGGATTCGTAAGGGCACATTACCCTTCAATTATCTTGGAGTGCCGGTGTTTCAAGGTCAACCTAAAGCTACATATTTCAGACCAATTGCTGATAAGATTAGGTGTAAACTTTCATCTTGGAAAGGCCTCCAGCTTTCTCAAGCAGCAAGGCTTCAGCTTATTTCTGCCACCATTCAAAGTCTTCTCATCTATAGCTTCCAGGTATATGAGTGGCCTCGTGCTTTGCTCCTCAAGGTTCAAGGTTGGACTCGGAATTTTTTCTGGACAGGTGACCCGTTAAAAAAGGGTTCAAACCTTATCGCCTGGTCCAAATGCTGCTCCCCAATTGATCAGGGAGGTCTTGGGTTAAAAAATATTTTCCATCTGAATAAAGCTCTTCTTCTTAAGCGTTGCTGGGAGGTTGTGGAAGGTTTATCTCCATCTGCTACATTTCTCAAGTGTAGATTCTTGAAAGCTGGATTGGCTCCATGCTCCTATTATAAAAAGTCATCAGTCTGGATTGGCTTAAAGAAGCAATGGCCCCTTATCATTTCTAATATTCGCTGGCTGATTGGTAATGGGGAGAACTTGTCATTCTGGAAAGACAATTGGTGTGGTTTGCCTCTATGCACAATGTACAATTTGGACCCTGATTTCTTGCCTTTTCTAAATGATCAGGTTAGTAAGTTTATTGTCGATAAAGAATGGGTGTTACCTCCTACCTTCTCTTCTTTATTCAAGGAAGCCTCGACAATTATTCTTGATACGGCTATCCCCTTGGATGATCAACCAGATCAAGCAGTCTGGCCTCATACTACCTCTGGTTCGCTCTCGGCTAGAGATGCCTACAGGTTTTTATCTCCTCATGGTGAGTCTGTTGGTTGGGGTAATCTTATCTGGCACAAGTCAATTCAGCCAAGGATGAGCCTAGTGGCTTGGCAAGTTTTTAATAGGCGAGCAAAGACCGACTGCTATCTACAGAAAATTGGTGTGCTCCTTTGCTCACAATGCTCCTTCTGCCTTGTAAGCACTGAAACCCTTACACATTTATTTCTGGAATGTGTTGTTATCAAAGCGCTTTGGGCTTGGATCTTCTCTCTTTTTCGTCTCCCTCCTTGGCAGGCCCAAGGGCTGGACTACTTGTTTTCGACCGAGGTGCTTCAGCGCCTTTCTCCTGCTTCGAAAATTCTGTGGAGATTAGTTGTCTGCAGCTTGCTTTGGCTTATTTGGACTGAGCGTAACTGTATTAGACACAATAACAGCATTTTTCATTTTGGGGGATTCATTCGGCGATTCACTTTAATTATGAAGGAAGCTGCCCAATTAGTTTTCAAGCCTCACACAAACTCGACTTCTGCTCTTCCAATTTTTCTCTTGCTGGAAATTTCTCCTCTGAGGCGCACTGCGCAGTCTTTTAAACCAGTGACCTGGTTAGCTCCTCCTGATCATTGGCTGAAAGTGAACACCGATGGGTCCTTCCGTGACGCTTCCAGGGCTGGATTTGGCGGAATCTTTAGAAATGCATCTGGAACTTTTATTGGAGCTTTCTCGGCTAGAGTTAATGTCCCTAGTGCCATTGATGCTGAGGTTCTCGCTGTTATTGAAGCCATTCAGGTGGCTTGGGTTAGGCGTTGGACTCACATTTGGTTGGAAACTGACTCCATGCTTGTTCTACACTATTTCCGTGCCCCAAATCTTATTCCTTGGAGACTCAAAACTCGCTGGTACAATTGTTTGCTTCAAGCTAAACATCTAATCCTTCAGGTGTCTCATATTTATAGAGAAGGCAATACTGTTGCAGATACGCTAGCCAACTATGGTGCTTCGAATGAAGGAGCTGTCTGGTGGGCTTCTATCCCTTCGTTCATCAATACTCAGTATAGTCATGATTTCAATTCACGCTCTAGTTATCGACTATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1353

Amino Acids

153.36

Weight (kDa)

9.32

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 476 - 735 4.6e-36 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 991 - 1074 9.3e-18 zinc-binding in reverse transcriptase
RVT_3 PF13456 1201 - 1319 1.5e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1418, 2640
Acc16I TGCGCA 1 cut(s) 3546
Acc36I ACCTGC 1 cut(s) 260
AccB7I CCANNNNNTGG 3 cut(s) 141, 1701, 3271
AciI CCGC 5 cut(s) 581, 839, 1066, 1308, 3645
AclWI GGATC 4 cut(s) 78, 889, 902, 3236
AcoI YGGCCR 2 cut(s) 1515, 2077
AcsI RAATTY 8 cut(s) 650, 680, 864, 1624, 1706, 2413, 3324, 3522
AcuI CTGAAG 4 cut(s) 788, 2297, 3284, 3884
AcvI CACGTG 1 cut(s) 480
AfaI GTAC 4 cut(s) 691, 1078, 2777, 3866
AfeI AGCGCT 3 cut(s) 400, 1567, 3216
AfiI CCNNNNNNNGG 9 cut(s) 141, 504, 691, 904, 1065, 1195, 1270, 1701, 3271
AflII CTTAAG 1 cut(s) 2543
AflIII ACRYGT 2 cut(s) 477, 1047
AhdI GACNNNNNGTC 1 cut(s) 1861
AjiI CACGTC 1 cut(s) 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 2603, 2635
Alw21I GWGCWC 2 cut(s) 434, 3135
Alw26I GTCTC 6 cut(s) 957, 1869, 2159, 3251, 3841, 3912
AlwI GGATC 4 cut(s) 78, 889, 902, 3236
Aor51HI AGCGCT 3 cut(s) 400, 1567, 3216
ApaI GGGCCC 2 cut(s) 520, 881
ApeKI GCWGC 9 cut(s) 401, 612, 667, 1042, 1538, 2303, 2475, 3348, 3455
ApoI RAATTY 8 cut(s) 650, 680, 864, 1624, 1706, 2413, 3324, 3522
AseI ATTAAT 1 cut(s) 1755
Asp700I GAANNNNTTC 2 cut(s) 2417, 3747
AspA2I CCTAGG 1 cut(s) 2158
AspLEI GCGC 8 cut(s) 401, 766, 1288, 1568, 3217, 3306, 3540, 3547
AsuC2I CCSGG 2 cut(s) 677, 761
AsuHPI GGTGA 8 cut(s) 356, 876, 1132, 1495, 1813, 1865, 2441, 3020
AsuII TTCGAA 2 cut(s) 3320, 3965
AsuNHI GCTAGC 1 cut(s) 3946
AvaII GGWCC 7 cut(s) 160, 326, 853, 907, 2466, 2785, 3612
AvrII CCTAGG 1 cut(s) 2158
BaeGI GKGCMC 4 cut(s) 520, 881, 2177, 3829
BaeI ACNNNNGTAYC 2 cut(s) 3856, 3889
BalI TGGCCA 2 cut(s) 1517, 2079
BamHI GGATCC 1 cut(s) 894
BanII GRGCYC 3 cut(s) 370, 520, 881
BauI CACGAG 2 cut(s) 406, 2376
BbrPI CACGTG 1 cut(s) 480
BbsI GAAGAC 3 cut(s) 331, 1167, 2333
Bbv12I GWGCWC 2 cut(s) 434, 3135
BbvCI CCTCAGC 1 cut(s) 3723
BbvI GCAGC 9 cut(s) 388, 599, 654, 1029, 1550, 2315, 2462, 3360, 3442
BccI CCATC 9 cut(s) 450, 528, 975, 1126, 1142, 1292, 2529, 2587, 3602
BceAI ACGGC 2 cut(s) 1321, 2922
BcgI CGANNNNNNTGC 6 cut(s) 724, 758, 2085, 2119, 3277, 3311
BciVI GTATCC 1 cut(s) 1295
BclI TGATCA 5 cut(s) 589, 2488, 2812, 2923, 3583
BcnI CCSGG 2 cut(s) 677, 761
BcoDI GTCTC 6 cut(s) 957, 1869, 2159, 3251, 3841, 3912
BfmI CTRYAG 5 cut(s) 171, 2350, 2986, 3116, 3346
BfoI RGCGCY 5 cut(s) 402, 767, 1569, 3218, 3307
BfrI CTTAAG 1 cut(s) 2543
BfuAI ACCTGC 1 cut(s) 260
BfuI GTATCC 1 cut(s) 1295
BglII AGATCT 1 cut(s) 1778
BlnI CCTAGG 1 cut(s) 2158
BlpI GCTNAGC 1 cut(s) 1937
Bme18I GGWCC 7 cut(s) 160, 326, 853, 907, 2466, 2785, 3612
BmeRI GACNNNNNGTC 1 cut(s) 1861
BmgBI CACGTC 1 cut(s) 1050
BmrI ACTGGG 1 cut(s) 1711
BmsI GCATC 5 cut(s) 532, 1779, 2971, 3671, 3709
BmtI GCTAGC 1 cut(s) 3950
BmuI ACTGGG 1 cut(s) 1711
BpiI GAAGAC 3 cut(s) 331, 1167, 2333
BplI GAGNNNNNCTC 2 cut(s) 601, 633
BpmI CTGGAG 2 cut(s) 1329, 2273
Bpu10I CCTNAGC 2 cut(s) 1715, 3723
Bpu1102I GCTNAGC 1 cut(s) 1937
Bpu14I TTCGAA 2 cut(s) 3320, 3965
BpuEI CTTGAG 3 cut(s) 2283, 2375, 2579
BpuMI CCSGG 2 cut(s) 677, 761
BsaAI YACGTR 2 cut(s) 480, 989
BsaBI GATNNNNATC 1 cut(s) 1152
BsaXI ACNNNNNCTCC 4 cut(s) 2190, 2220, 2714, 2744
Bsc4I CCNNNNNNNGG 9 cut(s) 141, 504, 691, 904, 1065, 1195, 1270, 1701, 3271
Bse118I RCCGGY 2 cut(s) 19, 2203
Bse1I ACTGG 4 cut(s) 1387, 1654, 1706, 3560
Bse3DI GCAATG 4 cut(s) 264, 1656, 1881, 2681
Bse8I GATNNNNATC 1 cut(s) 1152
BseGI GGATG 9 cut(s) 213, 547, 1153, 1279, 1389, 1770, 2062, 2926, 3066
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 9 cut(s) 141, 504, 691, 904, 1065, 1195, 1270, 1701, 3271
BseMI GCAATG 4 cut(s) 264, 1656, 1881, 2681
BseMII CTCAG 8 cut(s) 409, 656, 1789, 1912, 3363, 3524, 3714, 4028
BseNI ACTGG 4 cut(s) 1387, 1654, 1706, 3560
BseRI GAGGAG 7 cut(s) 172, 1857, 1904, 2378, 2991, 3519, 3567
BseSI GKGCMC 4 cut(s) 520, 881, 2177, 3829
BseXI GCAGC 9 cut(s) 388, 599, 654, 1029, 1550, 2315, 2462, 3360, 3442
BseYI CCCAGC 1 cut(s) 2553
BsgI GTGCAG 1 cut(s) 1028
Bsh1285I CGRYCG 1 cut(s) 3291
BsiEI CGRYCG 1 cut(s) 3291
BsiHKAI GWGCWC 2 cut(s) 434, 3135
BsiSI CCGG 5 cut(s) 20, 677, 760, 1942, 2204
BslFI GGGAC 2 cut(s) 173, 3689
BslI CCNNNNNNNGG 9 cut(s) 141, 504, 691, 904, 1065, 1195, 1270, 1701, 3271
BsmAI GTCTC 6 cut(s) 957, 1869, 2159, 3251, 3841, 3912
BsmBI CGTCTC 2 cut(s) 2159, 3251
BsmFI GGGAC 2 cut(s) 173, 3689
BsmI GAATGC 1 cut(s) 2031
Bsp119I TTCGAA 2 cut(s) 3320, 3965
Bsp120I GGGCCC 2 cut(s) 516, 877
Bsp1286I GDGCHC 7 cut(s) 370, 434, 520, 881, 2177, 3135, 3829
Bsp1407I TGTACA 1 cut(s) 2775
Bsp1720I GCTNAGC 1 cut(s) 1937
Bsp19I CCATGG 1 cut(s) 1695
BspACI CCGC 5 cut(s) 581, 839, 1066, 1308, 3645
BspCNI CTCAG 8 cut(s) 408, 655, 1788, 1911, 3364, 3525, 3715, 4027
BspHI TCATGA 3 cut(s) 237, 1093, 4024
BspMAI CTGCAG 1 cut(s) 3350
BspMI ACCTGC 1 cut(s) 260
BspOI GCTAGC 1 cut(s) 3950
BspPI GGATC 4 cut(s) 78, 889, 902, 3236
BspQI GCTCTTC 2 cut(s) 2541, 3504
BspT104I TTCGAA 2 cut(s) 3320, 3965
BspTI CTTAAG 1 cut(s) 2543
BsrDI GCAATG 4 cut(s) 264, 1656, 1881, 2681
BsrFI RCCGGY 2 cut(s) 19, 2203
BsrGI TGTACA 1 cut(s) 2775
BsrI ACTGG 4 cut(s) 1387, 1654, 1706, 3560
BssAI RCCGGY 2 cut(s) 19, 2203
BssSI CACGAG 2 cut(s) 406, 2376
BssT1I CCWWGG 8 cut(s) 1143, 1695, 2158, 2916, 3056, 3254, 3265, 3842
Bst2BI CACGAG 2 cut(s) 406, 2376
Bst4CI ACNGT 7 cut(s) 205, 224, 356, 694, 1261, 3383, 3934
Bst6I CTCTTC 5 cut(s) 190, 720, 2541, 2872, 3504
BstAFI CTTAAG 1 cut(s) 2543
BstAPI GCANNNNNTGC 1 cut(s) 251
BstAUI TGTACA 1 cut(s) 2775
BstBAI YACGTR 2 cut(s) 480, 989
BstBI TTCGAA 2 cut(s) 3320, 3965
BstC8I GCNNGC 6 cut(s) 1068, 2705, 3096, 3261, 3353, 3948
BstDSI CCRYGG 3 cut(s) 329, 1695, 2026
BstEII GGTNACC 2 cut(s) 1853, 2429
BstENI CCTNNNNNAGG 1 cut(s) 502
BstF5I GGATG 9 cut(s) 213, 547, 1153, 1279, 1389, 1770, 2062, 2926, 3066
BstH2I RGCGCY 5 cut(s) 402, 767, 1569, 3218, 3307
BstHHI GCGC 8 cut(s) 401, 766, 1288, 1568, 3217, 3306, 3540, 3547
BstMAI GTCTC 6 cut(s) 957, 1869, 2159, 3251, 3841, 3912
BstMCI CGRYCG 1 cut(s) 3291
BstNSI RCATGY 2 cut(s) 1982, 2126
BstPI GGTNACC 2 cut(s) 1853, 2429
BstSFI CTRYAG 5 cut(s) 171, 2350, 2986, 3116, 3346
BstSLI GKGCMC 4 cut(s) 520, 881, 2177, 3829
BstV1I GCAGC 9 cut(s) 388, 599, 654, 1029, 1550, 2315, 2462, 3360, 3442
BstV2I GAAGAC 3 cut(s) 331, 1167, 2333
BstX2I RGATCY 4 cut(s) 83, 894, 1778, 3228
BstXI CCANNNNNNTGG 3 cut(s) 1513, 3567, 3754
BstYI RGATCY 4 cut(s) 83, 894, 1778, 3228
BsuI GTATCC 1 cut(s) 1295
BtgI CCRYGG 3 cut(s) 329, 1695, 2026
BtrI CACGTC 1 cut(s) 1050
BtsCI GGATG 9 cut(s) 213, 547, 1153, 1279, 1389, 1770, 2062, 2926, 3066
BtsI GCAGTG 4 cut(s) 252, 1215, 1639, 3540
BtsIMutI CAGTG 9 cut(s) 201, 236, 252, 1215, 1257, 1639, 3168, 3540, 3567
BveI ACCTGC 1 cut(s) 260
Cac8I GCNNGC 6 cut(s) 1068, 2705, 3096, 3261, 3353, 3948
CciI TCATGA 3 cut(s) 237, 1093, 4024
CfoI GCGC 8 cut(s) 401, 766, 1288, 1568, 3217, 3306, 3540, 3547
Cfr10I RCCGGY 2 cut(s) 19, 2203
CseI GACGC 1 cut(s) 3632
CsiI ACCWGGT 2 cut(s) 849, 3566
Csp6I GTAC 4 cut(s) 690, 1077, 2776, 3865
CspCI CAANNNNNGTGG 4 cut(s) 644, 679, 2734, 2769
CviQI GTAC 4 cut(s) 690, 1077, 2776, 3865
DraI TTTAAA 1 cut(s) 3556
DriI GACNNNNNGTC 1 cut(s) 1861
EaeI YGGCCR 2 cut(s) 1515, 2077
Eam1104I CTCTTC 5 cut(s) 190, 720, 2541, 2872, 3504
Eam1105I GACNNNNNGTC 1 cut(s) 1861
EarI CTCTTC 5 cut(s) 190, 720, 2541, 2872, 3504
EciI GGCGGA 1 cut(s) 3660
Eco130I CCWWGG 8 cut(s) 1143, 1695, 2158, 2916, 3056, 3254, 3265, 3842
Eco147I AGGCCT 3 cut(s) 1468, 1685, 2286
Eco24I GRGCYC 3 cut(s) 370, 520, 881
Eco32I GATATC 1 cut(s) 1675
Eco47I GGWCC 7 cut(s) 160, 326, 853, 907, 2466, 2785, 3612
Eco47III AGCGCT 3 cut(s) 400, 1567, 3216
Eco57I CTGAAG 4 cut(s) 788, 2297, 3284, 3884
Eco72I CACGTG 1 cut(s) 480
Eco91I GGTNACC 2 cut(s) 1853, 2429
EcoNI CCTNNNNNAGG 1 cut(s) 502
EcoO109I RGGNCCY 6 cut(s) 160, 466, 516, 517, 878, 3612
EcoO65I GGTNACC 2 cut(s) 1853, 2429
EcoRI GAATTC 1 cut(s) 680
EcoRV GATATC 1 cut(s) 1675
EcoT14I CCWWGG 8 cut(s) 1143, 1695, 2158, 2916, 3056, 3254, 3265, 3842
EcoT22I ATGCAT 2 cut(s) 547, 3664
EcoT38I GRGCYC 3 cut(s) 370, 520, 881
ErhI CCWWGG 8 cut(s) 1143, 1695, 2158, 2916, 3056, 3254, 3265, 3842
Esp3I CGTCTC 2 cut(s) 2159, 3251
FalI AAGNNNNNCTT 2 cut(s) 2523, 2555
FaqI GGGAC 2 cut(s) 173, 3689
FauI CCCGC 1 cut(s) 1059
FbaI TGATCA 5 cut(s) 589, 2488, 2812, 2923, 3583
FokI GGATG 9 cut(s) 200, 554, 1160, 1286, 1376, 1757, 2069, 2933, 3073
FriOI GRGCYC 3 cut(s) 370, 520, 881
FspI TGCGCA 1 cut(s) 3546
GlaI GCGC 8 cut(s) 400, 765, 1287, 1567, 3216, 3305, 3539, 3546
GsaI CCCAGC 1 cut(s) 2557
GsuI CTGGAG 2 cut(s) 1329, 2273
HaeII RGCGCY 5 cut(s) 402, 767, 1569, 3218, 3307
HapII CCGG 5 cut(s) 20, 677, 760, 1942, 2204
HgaI GACGC 1 cut(s) 3632
HhaI GCGC 8 cut(s) 401, 766, 1288, 1568, 3217, 3306, 3540, 3547
Hin6I GCGC 8 cut(s) 399, 764, 1286, 1566, 3215, 3304, 3538, 3545
HinP1I GCGC 8 cut(s) 399, 764, 1286, 1566, 3215, 3304, 3538, 3545
HincII GTYRAC 2 cut(s) 281, 2219
HindII GTYRAC 2 cut(s) 281, 2219
HindIII AAGCTT 2 cut(s) 1369, 1934
HpaII CCGG 5 cut(s) 20, 677, 760, 1942, 2204
HphI GGTGA 8 cut(s) 356, 876, 1132, 1495, 1813, 1865, 2441, 3020
Hpy166II GTNNAC 7 cut(s) 281, 307, 483, 2120, 2219, 2267, 3601
Hpy8I GTNNAC 7 cut(s) 281, 307, 483, 2120, 2219, 2267, 3601
Hpy99I CGWCG 2 cut(s) 224, 279
HpyCH4III ACNGT 7 cut(s) 205, 224, 356, 694, 1261, 3383, 3934
HpyCH4IV ACGT 4 cut(s) 479, 988, 1049, 2152
HpySE526I ACGT 4 cut(s) 479, 988, 1049, 2152
HspAI GCGC 8 cut(s) 399, 764, 1286, 1566, 3215, 3304, 3538, 3545
Ksp22I TGATCA 5 cut(s) 589, 2488, 2812, 2923, 3583
LguI GCTCTTC 2 cut(s) 2541, 3504
Lsp1109I GCAGC 9 cut(s) 388, 599, 654, 1029, 1550, 2315, 2462, 3360, 3442
LweI GCATC 5 cut(s) 532, 1779, 2971, 3671, 3709
MabI ACCWGGT 2 cut(s) 849, 3566
MaeII ACGT 4 cut(s) 479, 988, 1049, 2152
MfeI CAATTG 5 cut(s) 1533, 2244, 2484, 2746, 3868
MflI RGATCY 4 cut(s) 83, 894, 1778, 3228
MhlI GDGCHC 7 cut(s) 370, 434, 520, 881, 2177, 3135, 3829
MlsI TGGCCA 2 cut(s) 1517, 2079
MluNI TGGCCA 2 cut(s) 1517, 2079
MlyI GAGTC 6 cut(s) 1157, 2401, 3020, 3769, 3791, 3843
MmeI TCCRAC 9 cut(s) 519, 545, 767, 894, 1310, 2173, 2384, 3752, 3768
Mox20I TGGCCA 2 cut(s) 1517, 2079
Mph1103I ATGCAT 2 cut(s) 547, 3664
MroXI GAANNNNTTC 2 cut(s) 2417, 3747
MscI TGGCCA 2 cut(s) 1517, 2079
MslI CAYNNNNRTG 1 cut(s) 3752
Msp20I TGGCCA 2 cut(s) 1517, 2079
MspCI CTTAAG 1 cut(s) 2543
MspI CCGG 5 cut(s) 20, 677, 760, 1942, 2204
MunI CAATTG 5 cut(s) 1533, 2244, 2484, 2746, 3868
Mva1269I GAATGC 1 cut(s) 2031
NciI CCSGG 2 cut(s) 677, 761
NcoI CCATGG 1 cut(s) 1695
NheI GCTAGC 1 cut(s) 3946
NmeAIII GCCGAG 2 cut(s) 2951, 3668
NmuCI GTSAC 5 cut(s) 152, 1853, 2429, 3562, 3620
NsbI TGCGCA 1 cut(s) 3546
NsiI ATGCAT 2 cut(s) 547, 3664
NspI RCATGY 2 cut(s) 1982, 2126
NspV TTCGAA 2 cut(s) 3320, 3965
PagI TCATGA 3 cut(s) 237, 1093, 4024
PceI AGGCCT 3 cut(s) 1468, 1685, 2286
PciSI GCTCTTC 2 cut(s) 2541, 3504
PctI GAATGC 1 cut(s) 2031
PdmI GAANNNNTTC 2 cut(s) 2417, 3747
PfeI GAWTC 8 cut(s) 975, 1426, 1545, 2164, 2604, 3420, 3432, 3648
PflMI CCANNNNNTGG 3 cut(s) 141, 1701, 3271
PfoI TCCNGGA 1 cut(s) 2052
PleI GAGTC 6 cut(s) 1157, 2401, 3019, 3769, 3791, 3843
PmaCI CACGTG 1 cut(s) 480
PmlI CACGTG 1 cut(s) 480
PpsI GAGTC 6 cut(s) 1157, 2401, 3019, 3769, 3791, 3843
Ppu21I YACGTR 2 cut(s) 480, 989
PpuMI RGGWCCY 2 cut(s) 160, 3612
PshBI ATTAAT 1 cut(s) 1755
PsiI TTATAA 2 cut(s) 1418, 2640
Psp5II RGGWCCY 2 cut(s) 160, 3612
PspCI CACGTG 1 cut(s) 480
PspEI GGTNACC 2 cut(s) 1853, 2429
PspFI CCCAGC 1 cut(s) 2553
PspOMI GGGCCC 2 cut(s) 516, 877
PspPPI RGGWCCY 2 cut(s) 160, 3612
PstI CTGCAG 1 cut(s) 3350
PsuI RGATCY 4 cut(s) 83, 894, 1778, 3228
RsaI GTAC 4 cut(s) 691, 1078, 2777, 3866
RsaNI GTAC 4 cut(s) 690, 1077, 2776, 3865
RseI CAYNNNNRTG 1 cut(s) 3752
SapI GCTCTTC 2 cut(s) 2541, 3504
SchI GAGTC 6 cut(s) 1157, 2401, 3020, 3769, 3791, 3843
SduI GDGCHC 7 cut(s) 370, 434, 520, 881, 2177, 3135, 3829
SexAI ACCWGGT 2 cut(s) 849, 3566
SfaNI GCATC 5 cut(s) 532, 1779, 2971, 3671, 3709
SfcI CTRYAG 5 cut(s) 171, 2350, 2986, 3116, 3346
SfuI TTCGAA 2 cut(s) 3320, 3965
SinI GGWCC 7 cut(s) 160, 326, 853, 907, 2466, 2785, 3612
SmiMI CAYNNNNRTG 1 cut(s) 3752
SmlI CTYRAG 4 cut(s) 2298, 2390, 2543, 2594
SmoI CTYRAG 4 cut(s) 2298, 2390, 2543, 2594
SseBI AGGCCT 3 cut(s) 1468, 1685, 2286
SsiI CCGC 5 cut(s) 581, 839, 1066, 1308, 3645
SspI AATATT 2 cut(s) 2515, 2698
StuI AGGCCT 3 cut(s) 1468, 1685, 2286
StyI CCWWGG 8 cut(s) 1143, 1695, 2158, 2916, 3056, 3254, 3265, 3842
TaaI ACNGT 7 cut(s) 205, 224, 356, 694, 1261, 3383, 3934
TaiI ACGT 4 cut(s) 482, 991, 1052, 2155
TaqII GACCGA 2 cut(s) 3119, 3305
TatI WGTACW 1 cut(s) 2775
TauI GCSGC 3 cut(s) 584, 842, 1311
TfiI GAWTC 8 cut(s) 975, 1426, 1545, 2164, 2604, 3420, 3432, 3648
TscAI CASTG 9 cut(s) 208, 236, 252, 1222, 1264, 1646, 3175, 3547, 3567
TseFI GTSAC 5 cut(s) 152, 1853, 2429, 3562, 3620
TseI GCWGC 9 cut(s) 401, 612, 667, 1042, 1538, 2303, 2475, 3348, 3455
Tsp45I GTSAC 5 cut(s) 152, 1853, 2429, 3562, 3620
TspGWI ACGGA 4 cut(s) 199, 318, 3608, 3812
TspRI CASTG 9 cut(s) 208, 236, 252, 1222, 1264, 1646, 3175, 3547, 3567
Van91I CCANNNNNTGG 3 cut(s) 141, 1701, 3271
Vha464I CTTAAG 1 cut(s) 2543
VpaK11BI GGWCC 7 cut(s) 160, 326, 853, 907, 2466, 2785, 3612
VspI ATTAAT 1 cut(s) 1755
XagI CCTNNNNNAGG 1 cut(s) 502
XapI RAATTY 8 cut(s) 650, 680, 864, 1624, 1706, 2413, 3324, 3522
XbaI TCTAGA 1 cut(s) 971
XceI RCATGY 2 cut(s) 1982, 2126
XcmI CCANNNNNNNNNTGG 1 cut(s) 1646
XmaJI CCTAGG 1 cut(s) 2158
XmnI GAANNNNTTC 2 cut(s) 2417, 3747
Zsp2I ATGCAT 2 cut(s) 547, 3664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.