Rw5G028420

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000093
Physical Location & Seq
Forward (+)
43028928 .. 43029521
594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028420.1

Sequence Viewer

Length: 594 bp
ATGGTTCTTGACTTCGGTGTGAGACTTGCAGATGAGTTTTTAACTGCGATTACTCCCACCCCGGTCCTCTCTTCTGATTTCATCACAAAATGGGAACCTCCCCCTCCGGATTTGGTGACTGTTAACTGTGATGCGGCCTGGGTCGATCCATCAAGTGGAGGTTTAGGTGTTGTGATTCGTGATCATAATGGCATATTGATTGCGGGTGGATCGAAGCAATCATGTCACGGATCGGTTGAAGTAGCTGAAGCTAGTGCAATTCTCCTTGAAGTCGAACTGGCAATTGAACAGAATCTGCAAAATGTTCGAATTGAATCAGATTCTTTGGAGGTTATAACTGATCTGAATTCCAAGAACGACAACAGAAACTGGAAAACCTCATCAATCATCGAAGAGATTAGATGGAGAAGTACCTTCTTCAATCGGATCTTCTGGGAATGGATTCCCCGAGAAGAGAATATGGCAGCTCATACAGCAGCCTCGCTTGGAAAGCGGTCGGTGGGTCTCTCCAGATGGGCCAACAGGCCACCACCAGCCTTGACTATGGTGCTCAGGAACGATGGTCTTCCCTGTCCTCCAGTTTCCTTAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

197

Amino Acids

21.7

Weight (kDa)

4.88

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 42 - 161 2.9e-23 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 335
AccB7I CCANNNNNTGG 1 cut(s) 155
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 3 cut(s) 134, 203, 493
AclWI GGATC 4 cut(s) 140, 217, 238, 434
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 1 cut(s) 412
AfiI CCNNNNNNNGG 1 cut(s) 155
AflII CTTAAG 1 cut(s) 586
AgsI TTSAA 5 cut(s) 239, 269, 287, 314, 421
AjnI CCWGG 1 cut(s) 137
AluBI AGCT 4 cut(s) 245, 251, 467, 591
AluI AGCT 4 cut(s) 245, 251, 467, 591
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 2 cut(s) 16, 509
AlwI GGATC 4 cut(s) 140, 217, 238, 434
AlwNI CAGNNNCTG 2 cut(s) 295, 369
Ama87I CYCGRG 1 cut(s) 447
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 3 cut(s) 135, 516, 524
ApeKI GCWGC 2 cut(s) 464, 476
ApoI RAATTY 1 cut(s) 346
Asp700I GAANNNNTTC 1 cut(s) 441
AspS9I GGNCC 2 cut(s) 64, 516
AsuC2I CCSGG 1 cut(s) 62
AsuHPI GGTGA 1 cut(s) 127
AsuII TTCGAA 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 476, 488
BccI CCATC 4 cut(s) 157, 396, 507, 554
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciT130I CCWGG 1 cut(s) 139
BclI TGATCA 1 cut(s) 181
BcnI CCSGG 1 cut(s) 62
BcoDI GTCTC 2 cut(s) 16, 509
BfaI CTAG 2 cut(s) 252, 592
BfrI CTTAAG 1 cut(s) 586
BisI GCNGC 3 cut(s) 135, 465, 477
BlsI GCNGC 3 cut(s) 136, 466, 478
Bme1390I CCNGG 2 cut(s) 62, 139
Bme18I GGWCC 1 cut(s) 64
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 64, 516
BmiI GGNNCC 1 cut(s) 96
BmrFI CCNGG 2 cut(s) 62, 139
BmsI GCATC 1 cut(s) 121
BpiI GAAGAC 1 cut(s) 557
BpmI CTGGAG 2 cut(s) 493, 561
Bpu10I CCTNAGC 1 cut(s) 551
Bpu14I TTCGAA 1 cut(s) 307
BpuMI CCSGG 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 509
BsaJI CCNNGG 2 cut(s) 60, 138
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 3 cut(s) 282, 374, 578
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 1 cut(s) 139
BseDI CCNNGG 2 cut(s) 60, 138
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 565
BseNI ACTGG 3 cut(s) 282, 374, 578
BseXI GCAGC 2 cut(s) 476, 488
Bsh1285I CGRYCG 1 cut(s) 497
BshFI GGCC 3 cut(s) 137, 518, 526
BsiEI CGRYCG 1 cut(s) 497
BsiHKAI GWGCWC 1 cut(s) 552
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 2 cut(s) 62, 107
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 16, 509
BsnI GGCC 3 cut(s) 137, 518, 526
Bso31I GGTCTC 1 cut(s) 509
BsoBI CYCGRG 1 cut(s) 447
Bsp119I TTCGAA 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 552
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 6 cut(s) 145, 181, 209, 230, 340, 426
BspACI CCGC 3 cut(s) 134, 203, 493
BspANI GGCC 3 cut(s) 137, 518, 526
BspCNI CTCAG 1 cut(s) 564
BspEI TCCGGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 96
BspPI GGATC 4 cut(s) 140, 217, 238, 434
BspT104I TTCGAA 1 cut(s) 307
BspTI CTTAAG 1 cut(s) 586
BspTNI GGTCTC 1 cut(s) 509
BsrI ACTGG 3 cut(s) 282, 374, 578
BssECI CCNNGG 2 cut(s) 60, 138
BssMI GATC 6 cut(s) 145, 181, 209, 230, 340, 426
Bst2UI CCWGG 1 cut(s) 139
Bst4CI ACNGT 2 cut(s) 121, 128
Bst6I CTCTTC 3 cut(s) 76, 387, 447
BstAFI CTTAAG 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 307
BstDEI CTNAG 1 cut(s) 551
BstKTI GATC 6 cut(s) 148, 184, 212, 233, 343, 429
BstMAI GTCTC 2 cut(s) 16, 509
BstMBI GATC 6 cut(s) 145, 181, 209, 230, 340, 426
BstMCI CGRYCG 1 cut(s) 497
BstMWI GCNNNNNNNGC 2 cut(s) 473, 490
BstNI CCWGG 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 60, 137
BstV1I GCAGC 2 cut(s) 476, 488
BstV2I GAAGAC 1 cut(s) 557
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 3 cut(s) 137, 518, 526
CaiI CAGNNNCTG 2 cut(s) 295, 369
Cfr13I GGNCC 2 cut(s) 64, 516
Csp6I GTAC 1 cut(s) 411
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviAII CATG 1 cut(s) 222
CviJI RGCY 9 cut(s) 137, 245, 251, 467, 479, 518, 526, 536, 591
CviKI_1 RGCY 9 cut(s) 137, 245, 251, 467, 479, 518, 526, 536, 591
CviQI GTAC 1 cut(s) 411
DdeI CTNAG 1 cut(s) 551
DpnI GATC 6 cut(s) 147, 183, 211, 232, 342, 428
DpnII GATC 6 cut(s) 145, 181, 209, 230, 340, 426
Eam1104I CTCTTC 3 cut(s) 76, 387, 447
EarI CTCTTC 3 cut(s) 76, 387, 447
Eco31I GGTCTC 1 cut(s) 509
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 447
EcoRI GAATTC 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 137
FaeI CATG 1 cut(s) 225
FaiI YATR 7 cut(s) 186, 194, 223, 335, 461, 471, 545
FatI CATG 1 cut(s) 221
FauI CCCGC 1 cut(s) 196
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 135, 465, 477
Fsp4HI GCNGC 3 cut(s) 135, 465, 477
FspBI CTAG 2 cut(s) 252, 592
GluI GCNGC 3 cut(s) 135, 465, 477
GsuI CTGGAG 2 cut(s) 493, 561
HaeIII GGCC 3 cut(s) 137, 518, 526
HapII CCGG 2 cut(s) 62, 107
Hin1II CATG 1 cut(s) 225
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 5 cut(s) 175, 292, 314, 320, 442
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 2 cut(s) 62, 107
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 76, 319, 345, 426
Hpy188III TCNNGA 5 cut(s) 8, 107, 179, 510, 553
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 2 cut(s) 121, 128
HpyCH4V TGCA 3 cut(s) 29, 257, 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 473, 490
HpyF3I CTNAG 1 cut(s) 551
Hsp92II CATG 1 cut(s) 225
Kpn2I TCCGGA 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 181
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 6 cut(s) 145, 181, 209, 230, 340, 426
Lsp1109I GCAGC 2 cut(s) 476, 488
LweI GCATC 1 cut(s) 121
MaeI CTAG 2 cut(s) 252, 592
MaeIII GTNAC 2 cut(s) 115, 224
MalI GATC 6 cut(s) 147, 183, 211, 232, 342, 428
MboI GATC 6 cut(s) 145, 181, 209, 230, 340, 426
MboII GAAGA 6 cut(s) 63, 404, 409, 421, 464, 557
MfeI CAATTG 1 cut(s) 282
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 1 cut(s) 552
MluCI AATT 4 cut(s) 258, 282, 309, 346
MnlI CCTC 8 cut(s) 77, 108, 114, 152, 322, 388, 490, 585
MroI TCCGGA 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 441
MseI TTAA 3 cut(s) 41, 123, 587
MspCI CTTAAG 1 cut(s) 586
MspI CCGG 2 cut(s) 62, 107
MspR9I CCNGG 2 cut(s) 62, 139
MunI CAATTG 1 cut(s) 282
MvaI CCWGG 1 cut(s) 139
MwoI GCNNNNNNNGC 2 cut(s) 473, 490
NciI CCSGG 1 cut(s) 62
NdeII GATC 6 cut(s) 145, 181, 209, 230, 340, 426
NlaIII CATG 1 cut(s) 225
NlaIV GGNNCC 1 cut(s) 96
NmuCI GTSAC 2 cut(s) 115, 224
NspV TTCGAA 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 5 cut(s) 175, 292, 314, 320, 442
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 3 cut(s) 136, 466, 478
PsiI TTATAA 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 137
PspGI CCWGG 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 96
PspPI GGNCC 2 cut(s) 64, 516
PstNI CAGNNNCTG 2 cut(s) 295, 369
PsuI RGATCY 1 cut(s) 426
RsaI GTAC 1 cut(s) 412
RsaNI GTAC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 41, 123, 587
SatI GCNGC 3 cut(s) 135, 465, 477
Sau3AI GATC 6 cut(s) 145, 181, 209, 230, 340, 426
Sau96I GGNCC 2 cut(s) 64, 516
ScrFI CCNGG 2 cut(s) 62, 139
SduI GDGCHC 1 cut(s) 552
SfaNI GCATC 1 cut(s) 121
SfuI TTCGAA 1 cut(s) 307
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 586
SmoI CTYRAG 1 cut(s) 586
Sse9I AATT 4 cut(s) 258, 282, 309, 346
SsiI CCGC 3 cut(s) 134, 203, 493
SspMI CTAG 2 cut(s) 252, 592
StyD4I CCNGG 2 cut(s) 60, 137
TaaI ACNGT 2 cut(s) 121, 128
TaqI TCGA 5 cut(s) 144, 212, 273, 307, 390
TasI AATT 4 cut(s) 258, 282, 309, 346
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 5 cut(s) 175, 292, 314, 320, 442
Tru1I TTAA 3 cut(s) 41, 123, 587
Tru9I TTAA 3 cut(s) 41, 123, 587
TseFI GTSAC 2 cut(s) 115, 224
TseI GCWGC 2 cut(s) 464, 476
Tsp45I GTSAC 2 cut(s) 115, 224
TspDTI ATGAA 1 cut(s) 70
TspGWI ACGGA 1 cut(s) 243
Van91I CCANNNNNTGG 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 2 cut(s) 252, 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.