FvH4_4g20431

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000093
Physical Location & Seq
Reverse (-)
23704863 .. 23705411
549 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20431.t1

Sequence Viewer

Length: 549 bp
ATGAAGGGAGGTGAGCCAAATCCTGCCATGGTTATTGATAAAGTCAACAGGAGCTATGCACACTGGATGGAGGCCAAGTCTATTGAAGAGAATGATGAAAATGGGCTCTCAAACCAAGCTCGATGTGATTTGTGGTGTCCCCCTCTTCCTTCTGAGGTAAAAATCAACATTGATGGTGCTTGGAAGTCCTCGAACCTCACTAGTGGGATAGGTATCATTGTGAGGAACTATAGAAGGTCTTCCATTGCTGGGGCAAGTCTGCATGGCTCTCACAACTCAGCAATGGAGGTGGAGGCAGATGCTGCTCTTAGAGCCCTCCAACTTGCTGCCCACCTCCGTTTGCAGAATATAGTGATTGAAAGTGACTGTCAAGACATCATCAAGACTTTAAACAGCTACCCTCTGACAACCAGCTGGAAAATCTCCCCCATTATTAGTAAGATAATGCATCTGAAGCCAATGTTCAAGGGATTGAGATGGAATTGGATTCCTAGAAAGGCAAACCGTGTGGCTGATGCCGCGGCTAAGCTTGCCAAGTTGAGATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

183

Amino Acids

20.25

Weight (kDa)

9.48

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 56 - 178 1.8e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 521
AciI CCGC 2 cut(s) 519, 521
AcuI CTGAAG 1 cut(s) 473
AfiI CCNNNNNNNGG 1 cut(s) 249
AgsI TTSAA 3 cut(s) 86, 359, 466
AhlI ACTAGT 1 cut(s) 200
AluBI AGCT 5 cut(s) 54, 119, 396, 414, 529
AluI AGCT 5 cut(s) 54, 119, 396, 414, 529
AlwNI CAGNNNCTG 1 cut(s) 302
AoxI GGCC 1 cut(s) 72
ApeKI GCWGC 2 cut(s) 302, 326
Asp700I GAANNNNTTC 1 cut(s) 238
AsuHPI GGTGA 1 cut(s) 23
BanII GRGCYC 2 cut(s) 108, 316
BbsI GAAGAC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 289, 313
BccI CCATC 3 cut(s) 61, 167, 471
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 2 cut(s) 201, 492
BfmI CTRYAG 1 cut(s) 229
BisI GCNGC 4 cut(s) 303, 327, 519, 522
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 4 cut(s) 304, 328, 520, 523
BmsI GCATC 3 cut(s) 289, 457, 505
BpiI GAAGAC 1 cut(s) 231
Bpu1102I GCTNAGC 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 27, 519
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 249
Bse1I ACTGG 1 cut(s) 68
Bse3DI GCAATG 2 cut(s) 243, 288
Bse8I GATNNNNATC 1 cut(s) 212
BseDI CCNNGG 2 cut(s) 27, 519
BseGI GGATG 1 cut(s) 72
BseJI GATNNNNATC 1 cut(s) 212
BseLI CCNNNNNNNGG 1 cut(s) 249
BseMI GCAATG 2 cut(s) 243, 288
BseMII CTCAG 2 cut(s) 144, 291
BseNI ACTGG 1 cut(s) 68
BseXI GCAGC 2 cut(s) 289, 313
BseYI CCCAGC 1 cut(s) 248
Bsh1236I CGCG 1 cut(s) 521
BshFI GGCC 1 cut(s) 74
BslFI GGGAC 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 123
BsnI GGCC 1 cut(s) 74
Bsp1286I GDGCHC 2 cut(s) 108, 316
Bsp1720I GCTNAGC 1 cut(s) 525
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 2 cut(s) 519, 521
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 2 cut(s) 145, 290
BspFNI CGCG 1 cut(s) 521
BsrDI GCAATG 2 cut(s) 243, 288
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 27, 519
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 2 cut(s) 368, 506
Bst6I CTCTTC 2 cut(s) 81, 150
BstAPI GCANNNNNTGC 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 4 cut(s) 153, 277, 308, 525
BstDSI CCRYGG 2 cut(s) 27, 519
BstF5I GGATG 1 cut(s) 72
BstFNI CGCG 1 cut(s) 521
BstMWI GCNNNNNNNGC 5 cut(s) 302, 311, 454, 518, 530
BstSFI CTRYAG 1 cut(s) 229
BstUI CGCG 1 cut(s) 521
BstV1I GCAGC 2 cut(s) 289, 313
BstV2I GAAGAC 1 cut(s) 231
BsuRI GGCC 1 cut(s) 74
BtgI CCRYGG 2 cut(s) 27, 519
BtsCI GGATG 1 cut(s) 72
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 1 cut(s) 531
CaiI CAGNNNCTG 1 cut(s) 302
Cfr42I CCGCGG 1 cut(s) 522
CspCI CAANNNNNGTGG 4 cut(s) 270, 305, 489, 524
CviAII CATG 2 cut(s) 28, 263
DdeI CTNAG 4 cut(s) 153, 277, 308, 525
DraI TTTAAA 1 cut(s) 390
Eam1104I CTCTTC 2 cut(s) 81, 150
EarI CTCTTC 2 cut(s) 81, 150
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 108, 316
Eco57I CTGAAG 1 cut(s) 473
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 1 cut(s) 450
EcoT38I GRGCYC 2 cut(s) 108, 316
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 2 cut(s) 31, 266
FaiI YATR 5 cut(s) 29, 57, 231, 264, 350
FaqI GGGAC 1 cut(s) 123
FatI CATG 2 cut(s) 27, 262
Fnu4HI GCNGC 4 cut(s) 303, 327, 519, 522
FokI GGATG 1 cut(s) 79
FriOI GRGCYC 2 cut(s) 108, 316
Fsp4HI GCNGC 4 cut(s) 303, 327, 519, 522
FspBI CTAG 2 cut(s) 201, 492
GluI GCNGC 4 cut(s) 303, 327, 519, 522
GsaI CCCAGC 1 cut(s) 252
HaeIII GGCC 1 cut(s) 74
Hin1II CATG 2 cut(s) 31, 266
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 1 cut(s) 487
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 154, 405, 453
Hpy188III TCNNGA 2 cut(s) 371, 382
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 2 cut(s) 159, 228
HpyCH4III ACNGT 2 cut(s) 368, 506
HpyCH4V TGCA 4 cut(s) 59, 262, 343, 448
HpyF10VI GCNNNNNNNGC 5 cut(s) 302, 311, 454, 518, 530
HpyF3I CTNAG 4 cut(s) 153, 277, 308, 525
Hsp92II CATG 2 cut(s) 31, 266
KspI CCGCGG 1 cut(s) 522
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 6 cut(s) 34, 36, 49, 234, 400, 424
Lsp1109I GCAGC 2 cut(s) 289, 313
LweI GCATC 3 cut(s) 289, 457, 505
MaeI CTAG 2 cut(s) 201, 492
MaeIII GTNAC 1 cut(s) 362
MboII GAAGA 3 cut(s) 98, 137, 231
MhlI GDGCHC 2 cut(s) 108, 316
MluCI AATT 1 cut(s) 481
MmeI TCCRAC 1 cut(s) 343
Mph1103I ATGCAT 1 cut(s) 450
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 1 cut(s) 389
MspA1I CMGCKG 2 cut(s) 414, 521
MvnI CGCG 1 cut(s) 521
MwoI GCNNNNNNNGC 5 cut(s) 302, 311, 454, 518, 530
NcoI CCATGG 1 cut(s) 27
NlaIII CATG 2 cut(s) 31, 266
NmuCI GTSAC 1 cut(s) 362
NsiI ATGCAT 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 487
PkrI GCNGC 4 cut(s) 304, 328, 520, 523
PspFI CCCAGC 1 cut(s) 248
PstNI CAGNNNCTG 1 cut(s) 302
PvuII CAGCTG 1 cut(s) 414
SacII CCGCGG 1 cut(s) 522
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 4 cut(s) 303, 327, 519, 522
SduI GDGCHC 2 cut(s) 108, 316
SfaNI GCATC 3 cut(s) 289, 457, 505
SfcI CTRYAG 1 cut(s) 229
Sfr303I CCGCGG 1 cut(s) 522
SgrBI CCGCGG 1 cut(s) 522
SpeI ACTAGT 1 cut(s) 200
Sse9I AATT 1 cut(s) 481
SsiI CCGC 2 cut(s) 519, 521
SspMI CTAG 2 cut(s) 201, 492
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 368, 506
TaqI TCGA 2 cut(s) 121, 191
TasI AATT 1 cut(s) 481
TauI GCSGC 2 cut(s) 521, 524
TfiI GAWTC 1 cut(s) 487
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 1 cut(s) 362
TseI GCWGC 2 cut(s) 302, 326
Tsp45I GTSAC 1 cut(s) 362
TspDTI ATGAA 2 cut(s) 17, 111
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 1 cut(s) 68
XmnI GAANNNNTTC 1 cut(s) 238
XspI CTAG 2 cut(s) 201, 492
Zsp2I ATGCAT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.