ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 479 |
| AccI | GTMKAC | 1 cut(s) | 19 |
| AciI | CCGC | 3 cut(s) | 587, 655, 916 |
| AcoI | YGGCCR | 2 cut(s) | 283, 709 |
| AcsI | RAATTY | 2 cut(s) | 74, 611 |
| AcuI | CTGAAG | 1 cut(s) | 312 |
| AcyI | GRCGYC | 1 cut(s) | 993 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 373, 430 |
| AflIII | ACRYGT | 1 cut(s) | 552 |
| AgsI | TTSAA | 5 cut(s) | 14, 45, 236, 616, 718 |
| AjnI | CCWGG | 2 cut(s) | 475, 983 |
| AluBI | AGCT | 5 cut(s) | 113, 258, 330, 778, 1010 |
| AluI | AGCT | 5 cut(s) | 113, 258, 330, 778, 1010 |
| Alw21I | GWGCWC | 1 cut(s) | 451 |
| Alw26I | GTCTC | 2 cut(s) | 758, 802 |
| Alw44I | GTGCAC | 1 cut(s) | 447 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 974 |
| AoxI | GGCC | 3 cut(s) | 283, 709, 957 |
| ApaLI | GTGCAC | 1 cut(s) | 447 |
| ApeKI | GCWGC | 2 cut(s) | 802, 1007 |
| ApoI | RAATTY | 2 cut(s) | 74, 611 |
| AspS9I | GGNCC | 1 cut(s) | 957 |
| AsuHPI | GGTGA | 3 cut(s) | 92, 236, 833 |
| AxyI | CCTNAGG | 1 cut(s) | 1028 |
| BaeGI | GKGCMC | 1 cut(s) | 451 |
| BalI | TGGCCA | 1 cut(s) | 285 |
| BanI | GGYRCC | 1 cut(s) | 479 |
| BbsI | GAAGAC | 1 cut(s) | 996 |
| Bbv12I | GWGCWC | 1 cut(s) | 451 |
| BbvI | GCAGC | 2 cut(s) | 789, 1019 |
| BccI | CCATC | 4 cut(s) | 275, 463, 688, 1010 |
| BceAI | ACGGC | 1 cut(s) | 696 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 329, 363 |
| BciT130I | CCWGG | 2 cut(s) | 477, 985 |
| BcoDI | GTCTC | 2 cut(s) | 758, 802 |
| BfaI | CTAG | 2 cut(s) | 668, 1019 |
| BisI | GCNGC | 3 cut(s) | 803, 916, 1008 |
| BlsI | GCNGC | 3 cut(s) | 804, 917, 1009 |
| Bme1390I | CCNGG | 2 cut(s) | 477, 985 |
| BmgT120I | GGNCC | 1 cut(s) | 957 |
| BmiI | GGNNCC | 1 cut(s) | 481 |
| BmrFI | CCNGG | 2 cut(s) | 477, 985 |
| BmrI | ACTGGG | 1 cut(s) | 382 |
| BmsI | GCATC | 1 cut(s) | 967 |
| BmuI | ACTGGG | 1 cut(s) | 382 |
| BpiI | GAAGAC | 1 cut(s) | 996 |
| BpuEI | CTTGAG | 1 cut(s) | 791 |
| BsaAI | YACGTR | 1 cut(s) | 942 |
| BsaHI | GRCGYC | 1 cut(s) | 993 |
| BsaJI | CCNNGG | 3 cut(s) | 475, 486, 984 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 615, 645 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 373, 430 |
| Bse1I | ACTGG | 2 cut(s) | 377, 389 |
| Bse21I | CCTNAGG | 1 cut(s) | 1028 |
| BseBI | CCWGG | 2 cut(s) | 477, 985 |
| BseDI | CCNNGG | 3 cut(s) | 475, 486, 984 |
| BseGI | GGATG | 2 cut(s) | 87, 680 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 373, 430 |
| BseMII | CTCAG | 2 cut(s) | 708, 1019 |
| BseNI | ACTGG | 2 cut(s) | 377, 389 |
| BseSI | GKGCMC | 1 cut(s) | 451 |
| BseXI | GCAGC | 2 cut(s) | 789, 1019 |
| BsgI | GTGCAG | 2 cut(s) | 759, 971 |
| BshFI | GGCC | 3 cut(s) | 285, 711, 959 |
| BshNI | GGYRCC | 1 cut(s) | 479 |
| BsiHKAI | GWGCWC | 1 cut(s) | 451 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 373, 430 |
| BsmAI | GTCTC | 2 cut(s) | 758, 802 |
| BsmBI | CGTCTC | 1 cut(s) | 758 |
| BsmI | GAATGC | 1 cut(s) | 168 |
| BsnI | GGCC | 3 cut(s) | 285, 711, 959 |
| Bsp1286I | GDGCHC | 1 cut(s) | 451 |
| Bsp143I | GATC | 3 cut(s) | 61, 523, 757 |
| BspACI | CCGC | 3 cut(s) | 587, 655, 916 |
| BspANI | GGCC | 3 cut(s) | 285, 711, 959 |
| BspCNI | CTCAG | 2 cut(s) | 707, 1020 |
| BspHI | TCATGA | 1 cut(s) | 135 |
| BspLI | GGNNCC | 1 cut(s) | 481 |
| BspT107I | GGYRCC | 1 cut(s) | 479 |
| BsrI | ACTGG | 2 cut(s) | 377, 389 |
| BssECI | CCNNGG | 3 cut(s) | 475, 486, 984 |
| BssMI | GATC | 3 cut(s) | 61, 523, 757 |
| BssNI | GRCGYC | 1 cut(s) | 993 |
| BssT1I | CCWWGG | 1 cut(s) | 486 |
| Bst2UI | CCWGG | 2 cut(s) | 477, 985 |
| Bst4CI | ACNGT | 1 cut(s) | 752 |
| Bst6I | CTCTTC | 2 cut(s) | 806, 844 |
| BstACI | GRCGYC | 1 cut(s) | 993 |
| BstBAI | YACGTR | 1 cut(s) | 942 |
| BstC8I | GCNNGC | 1 cut(s) | 348 |
| BstDEI | CTNAG | 2 cut(s) | 694, 1028 |
| BstF5I | GGATG | 2 cut(s) | 87, 680 |
| BstKTI | GATC | 3 cut(s) | 64, 526, 760 |
| BstMAI | GTCTC | 2 cut(s) | 758, 802 |
| BstMBI | GATC | 3 cut(s) | 61, 523, 757 |
| BstNI | CCWGG | 2 cut(s) | 477, 985 |
| BstNSI | RCATGY | 3 cut(s) | 556, 587, 967 |
| BstSCI | CCNGG | 2 cut(s) | 475, 983 |
| BstSLI | GKGCMC | 1 cut(s) | 451 |
| BstV1I | GCAGC | 2 cut(s) | 789, 1019 |
| BstV2I | GAAGAC | 1 cut(s) | 996 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1028 |
| BsuRI | GGCC | 3 cut(s) | 285, 711, 959 |
| BtgZI | GCGATG | 1 cut(s) | 988 |
| BtsCI | GGATG | 2 cut(s) | 87, 680 |
| BtsI | GCAGTG | 2 cut(s) | 509, 959 |
| BtsIMutI | CAGTG | 4 cut(s) | 370, 509, 730, 959 |
| Cac8I | GCNNGC | 1 cut(s) | 348 |
| CaiI | CAGNNNCTG | 1 cut(s) | 974 |
| CciI | TCATGA | 1 cut(s) | 135 |
| Cfr13I | GGNCC | 1 cut(s) | 957 |
| CseI | GACGC | 1 cut(s) | 1001 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 33, 68 |
| DdeI | CTNAG | 2 cut(s) | 694, 1028 |
| DpnI | GATC | 3 cut(s) | 63, 525, 759 |
| DpnII | GATC | 3 cut(s) | 61, 523, 757 |
| DraI | TTTAAA | 1 cut(s) | 537 |
| EaeI | YGGCCR | 2 cut(s) | 283, 709 |
| Eam1104I | CTCTTC | 2 cut(s) | 806, 844 |
| EarI | CTCTTC | 2 cut(s) | 806, 844 |
| Eco130I | CCWWGG | 1 cut(s) | 486 |
| Eco57I | CTGAAG | 1 cut(s) | 312 |
| Eco81I | CCTNAGG | 1 cut(s) | 1028 |
| EcoRI | GAATTC | 1 cut(s) | 611 |
| EcoRII | CCWGG | 2 cut(s) | 475, 983 |
| EcoT14I | CCWWGG | 1 cut(s) | 486 |
| ErhI | CCWWGG | 1 cut(s) | 486 |
| Esp3I | CGTCTC | 1 cut(s) | 758 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 351, 383 |
| FblI | GTMKAC | 1 cut(s) | 19 |
| Fnu4HI | GCNGC | 3 cut(s) | 803, 916, 1008 |
| FokI | GGATG | 2 cut(s) | 74, 667 |
| Fsp4HI | GCNGC | 3 cut(s) | 803, 916, 1008 |
| FspBI | CTAG | 2 cut(s) | 668, 1019 |
| GluI | GCNGC | 3 cut(s) | 803, 916, 1008 |
| HaeIII | GGCC | 3 cut(s) | 285, 711, 959 |
| HgaI | GACGC | 1 cut(s) | 1001 |
| Hin1I | GRCGYC | 1 cut(s) | 993 |
| HincII | GTYRAC | 2 cut(s) | 301, 502 |
| HindII | GTYRAC | 2 cut(s) | 301, 502 |
| HinfI | GANTC | 2 cut(s) | 568, 971 |
| HphI | GGTGA | 3 cut(s) | 92, 236, 833 |
| Hpy166II | GTNNAC | 4 cut(s) | 20, 301, 449, 502 |
| Hpy188I | TCNGA | 4 cut(s) | 230, 292, 416, 976 |
| Hpy188III | TCNNGA | 8 cut(s) | 136, 311, 565, 755, 808, 899, 1019, 1027 |
| Hpy8I | GTNNAC | 4 cut(s) | 20, 301, 449, 502 |
| HpyAV | CCTTC | 1 cut(s) | 377 |
| HpyCH4III | ACNGT | 1 cut(s) | 752 |
| HpyCH4IV | ACGT | 1 cut(s) | 941 |
| HpyCH4V | TGCA | 9 cut(s) | 80, 325, 350, 449, 514, 728, 740, 952, 980 |
| HpyF3I | CTNAG | 2 cut(s) | 694, 1028 |
| HpySE526I | ACGT | 1 cut(s) | 941 |
| Hsp92I | GRCGYC | 1 cut(s) | 993 |
| Kzo9I | GATC | 3 cut(s) | 61, 523, 757 |
| LmnI | GCTCC | 1 cut(s) | 118 |
| Lsp1109I | GCAGC | 2 cut(s) | 789, 1019 |
| LweI | GCATC | 1 cut(s) | 967 |
| MaeI | CTAG | 2 cut(s) | 668, 1019 |
| MaeII | ACGT | 1 cut(s) | 941 |
| MaeIII | GTNAC | 3 cut(s) | 35, 94, 182 |
| MalI | GATC | 3 cut(s) | 63, 525, 759 |
| MboI | GATC | 3 cut(s) | 61, 523, 757 |
| MboII | GAAGA | 6 cut(s) | 217, 328, 752, 823, 831, 1001 |
| MfeI | CAATTG | 1 cut(s) | 527 |
| MhlI | GDGCHC | 1 cut(s) | 451 |
| MlsI | TGGCCA | 1 cut(s) | 285 |
| MluNI | TGGCCA | 1 cut(s) | 285 |
| MmeI | TCCRAC | 2 cut(s) | 253, 708 |
| MnlI | CCTC | 8 cut(s) | 77, 136, 257, 374, 613, 817, 1006, 1023 |
| Mox20I | TGGCCA | 1 cut(s) | 285 |
| MscI | TGGCCA | 1 cut(s) | 285 |
| MseI | TTAA | 2 cut(s) | 536, 891 |
| Msp20I | TGGCCA | 1 cut(s) | 285 |
| MspR9I | CCNGG | 2 cut(s) | 477, 985 |
| MunI | CAATTG | 1 cut(s) | 527 |
| Mva1269I | GAATGC | 1 cut(s) | 168 |
| MvaI | CCWGG | 2 cut(s) | 477, 985 |
| NdeII | GATC | 3 cut(s) | 61, 523, 757 |
| NlaIV | GGNNCC | 1 cut(s) | 481 |
| NmuCI | GTSAC | 1 cut(s) | 182 |
| NspI | RCATGY | 3 cut(s) | 556, 587, 967 |
| PagI | TCATGA | 1 cut(s) | 135 |
| PciI | ACATGT | 1 cut(s) | 552 |
| PctI | GAATGC | 1 cut(s) | 168 |
| PfeI | GAWTC | 2 cut(s) | 568, 971 |
| PkrI | GCNGC | 3 cut(s) | 804, 917, 1009 |
| Ppu21I | YACGTR | 1 cut(s) | 942 |
| PscI | ACATGT | 1 cut(s) | 552 |
| Psp6I | CCWGG | 2 cut(s) | 475, 983 |
| PspGI | CCWGG | 2 cut(s) | 475, 983 |
| PspN4I | GGNNCC | 1 cut(s) | 481 |
| PspPI | GGNCC | 1 cut(s) | 957 |
| PstNI | CAGNNNCTG | 1 cut(s) | 974 |
| SaqAI | TTAA | 2 cut(s) | 536, 891 |
| SatI | GCNGC | 3 cut(s) | 803, 916, 1008 |
| Sau3AI | GATC | 3 cut(s) | 61, 523, 757 |
| Sau96I | GGNCC | 1 cut(s) | 957 |
| ScrFI | CCNGG | 2 cut(s) | 477, 985 |
| SduI | GDGCHC | 1 cut(s) | 451 |
| SfaNI | GCATC | 1 cut(s) | 967 |
| SmlI | CTYRAG | 1 cut(s) | 806 |
| SmoI | CTYRAG | 1 cut(s) | 806 |
| SsiI | CCGC | 3 cut(s) | 587, 655, 916 |
| SspI | AATATT | 2 cut(s) | 198, 561 |
| SspMI | CTAG | 2 cut(s) | 668, 1019 |
| StyD4I | CCNGG | 2 cut(s) | 475, 983 |
| StyI | CCWWGG | 1 cut(s) | 486 |
| TaaI | ACNGT | 1 cut(s) | 752 |
| TaiI | ACGT | 1 cut(s) | 944 |
| TaqI | TCGA | 5 cut(s) | 151, 339, 571, 756, 858 |
| TauI | GCSGC | 1 cut(s) | 918 |
| TfiI | GAWTC | 2 cut(s) | 568, 971 |
| Tru1I | TTAA | 2 cut(s) | 536, 891 |
| Tru9I | TTAA | 2 cut(s) | 536, 891 |
| TscAI | CASTG | 4 cut(s) | 377, 516, 730, 959 |
| TseFI | GTSAC | 1 cut(s) | 182 |
| TseI | GCWGC | 2 cut(s) | 802, 1007 |
| Tsp45I | GTSAC | 1 cut(s) | 182 |
| TspDTI | ATGAA | 6 cut(s) | 76, 124, 146, 322, 482, 590 |
| TspRI | CASTG | 4 cut(s) | 377, 516, 730, 959 |
| VneI | GTGCAC | 1 cut(s) | 447 |
| XapI | RAATTY | 2 cut(s) | 74, 611 |
| XbaI | TCTAGA | 1 cut(s) | 1018 |
| XceI | RCATGY | 3 cut(s) | 556, 587, 967 |
| XmiI | GTMKAC | 1 cut(s) | 19 |
| XspI | CTAG | 2 cut(s) | 668, 1019 |