Rorug06G0241000

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000093
Physical Location & Seq
Reverse (-)
38443129 .. 38443971
843 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0241000.1

Sequence Viewer

Length: 843 bp
ATGGCTTTCCACACTCGCTCCAACAGCTTTCCCTCAAGGCCACACCCACTGTTTCAAGAAATTGATGAGCATTTGTGCAGATTGAAGTCTTCTGAGGCCACCTCCACATCTTCATCATCAATAAGCCACAAACTAAGCAGTCTCCAAGACTTGCATGATTGTGTCGATAGGTTGCTTCAATTGCCTCTCACCCAAAAGGCCTTGGCACAAGAGCAGCATCAGAAATGGGCTAATGAGCTATTAGATGGATCTCTCAGGATCTTGGATGTTTGTAGCACTTCCAAAGACGCCCTGATGAAAACCAAGGAAAACGTACAAGATCTTCAATCAATCATTCGAAGAAAGCGTGGAGATGAATCTGCAGTACTCAAGAGTGAAGCAAGGAAATACTTGATATCCAGGAAGACAGTGAAAAAGGCTCTCCACAAAGCTTTGGGGAACATGAAGGGAATGGCAAACAGATCTTCGTCTTCCTGCAACAAGGATCAAGAGACTATAACCATCGTGAGCAAGCTGAGAGATGTGGAAGCAGTCACTCTCGCCATGTTTGAATCAGTTCTATCCTTCATCTCAGGGCCAAAGTCAAAGCCTTACAGCTGGTCTTTGGTTTCCAAGATGTTGCAGTCAAATAGAATAGCTTGCGAGGAAGAAACAAAAGTAAATGAATTTACAGAGGTGGATGCTGCATTGAAGTCACTCAAGAGTGGTGACAACACACACAACCAGCTTAACAATCTGGAATCATGCATTCAAGACCAAGAAGAAGCACTTGATTGTCTATATAGGCAATTGATCAAAACAAGAGTCTCCCTTCTCAACATATCCTCAACCACTAGATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

280

Amino Acids

31.56

Weight (kDa)

8.88

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BPS1 PF03087 44 - 274 2e-83 Protein BPS1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 256, 266, 492
AcsI RAATTY 1 cut(s) 665
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 315, 366
AgsI TTSAA 7 cut(s) 56, 85, 179, 326, 551, 691, 752
AjnI CCWGG 1 cut(s) 398
AluBI AGCT 7 cut(s) 27, 238, 431, 514, 597, 638, 727
AluI AGCT 7 cut(s) 27, 238, 431, 514, 597, 638, 727
Alw26I GTCTC 3 cut(s) 146, 485, 811
AlwI GGATC 3 cut(s) 256, 266, 492
AoxI GGCC 4 cut(s) 38, 96, 198, 575
ApeKI GCWGC 2 cut(s) 214, 683
ApoI RAATTY 1 cut(s) 665
ArsI GACNNNNNNTTYG 2 cut(s) 789, 821
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 181, 719
AsuII TTCGAA 1 cut(s) 337
BarI GAAGNNNNNNTAC 2 cut(s) 306, 338
BbsI GAAGAC 3 cut(s) 81, 410, 462
BbvI GCAGC 2 cut(s) 226, 670
BccI CCATC 2 cut(s) 239, 509
BciT130I CCWGG 1 cut(s) 400
BclI TGATCA 1 cut(s) 792
BcoDI GTCTC 3 cut(s) 146, 485, 811
BfaI CTAG 1 cut(s) 834
BfmI CTRYAG 1 cut(s) 360
BglII AGATCT 2 cut(s) 319, 461
BisI GCNGC 2 cut(s) 215, 684
BlsI GCNGC 2 cut(s) 216, 685
BmcAI AGTACT 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 575
BmrFI CCNGG 1 cut(s) 400
BmsI GCATC 2 cut(s) 226, 670
BpiI GAAGAC 3 cut(s) 81, 410, 462
BplI GAGNNNNNCTC 2 cut(s) 86, 118
Bpu14I TTCGAA 1 cut(s) 337
BpuEI CTTGAG 3 cut(s) 19, 353, 683
BsaHI GRCGYC 1 cut(s) 288
BsaJI CCNNGG 2 cut(s) 201, 303
BsaXI ACNNNNNCTCC 1 cut(s) 32
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 2 cut(s) 201, 303
BseGI GGATG 2 cut(s) 271, 685
BseMII CTCAG 4 cut(s) 84, 268, 506, 585
BseXI GCAGC 2 cut(s) 226, 670
BsgI GTGCAG 1 cut(s) 97
BshFI GGCC 4 cut(s) 40, 98, 200, 577
BsmAI GTCTC 3 cut(s) 146, 485, 811
BsmI GAATGC 1 cut(s) 747
BsnI GGCC 4 cut(s) 40, 98, 200, 577
Bsp119I TTCGAA 1 cut(s) 337
Bsp143I GATC 6 cut(s) 248, 258, 319, 461, 484, 792
BspANI GGCC 4 cut(s) 40, 98, 200, 577
BspCNI CTCAG 4 cut(s) 85, 267, 507, 584
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 3 cut(s) 256, 266, 492
BspT104I TTCGAA 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 201, 303
BssMI GATC 6 cut(s) 248, 258, 319, 461, 484, 792
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 2 cut(s) 201, 303
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 2 cut(s) 51, 409
BstACI GRCGYC 1 cut(s) 288
BstBI TTCGAA 1 cut(s) 337
BstC8I GCNNGC 2 cut(s) 512, 640
BstDEI CTNAG 5 cut(s) 93, 134, 254, 515, 571
BstF5I GGATG 2 cut(s) 271, 685
BstKTI GATC 6 cut(s) 251, 261, 322, 464, 487, 795
BstMAI GTCTC 3 cut(s) 146, 485, 811
BstMBI GATC 6 cut(s) 248, 258, 319, 461, 484, 792
BstMWI GCNNNNNNNGC 2 cut(s) 24, 181
BstNI CCWGG 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 398
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 226, 670
BstV2I GAAGAC 3 cut(s) 81, 410, 462
BstX2I RGATCY 4 cut(s) 248, 258, 319, 461
BstYI RGATCY 4 cut(s) 248, 258, 319, 461
BsuRI GGCC 4 cut(s) 40, 98, 200, 577
BtsCI GGATG 2 cut(s) 271, 685
BtsIMutI CAGTG 2 cut(s) 47, 414
Cac8I GCNNGC 2 cut(s) 512, 640
Cfr13I GGNCC 1 cut(s) 575
CseI GACGC 1 cut(s) 296
Csp6I GTAC 2 cut(s) 314, 365
CviAII CATG 4 cut(s) 155, 442, 544, 744
CviQI GTAC 2 cut(s) 314, 365
DdeI CTNAG 5 cut(s) 93, 134, 254, 515, 571
DpnI GATC 6 cut(s) 250, 260, 321, 463, 486, 794
DpnII GATC 6 cut(s) 248, 258, 319, 461, 484, 792
Eco130I CCWWGG 2 cut(s) 201, 303
Eco147I AGGCCT 1 cut(s) 200
Eco32I GATATC 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 398
EcoRV GATATC 1 cut(s) 396
EcoT14I CCWWGG 2 cut(s) 201, 303
EcoT22I ATGCAT 1 cut(s) 749
ErhI CCWWGG 2 cut(s) 201, 303
FaeI CATG 4 cut(s) 158, 445, 547, 747
FaiI YATR 8 cut(s) 156, 443, 497, 545, 745, 781, 783, 821
FalI AAGNNNNNCTT 2 cut(s) 753, 785
FatI CATG 4 cut(s) 154, 441, 543, 743
FbaI TGATCA 1 cut(s) 792
Fnu4HI GCNGC 2 cut(s) 215, 684
FokI GGATG 2 cut(s) 278, 692
Fsp4HI GCNGC 2 cut(s) 215, 684
FspBI CTAG 1 cut(s) 834
GluI GCNGC 2 cut(s) 215, 684
HaeIII GGCC 4 cut(s) 40, 98, 200, 577
HgaI GACGC 1 cut(s) 296
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 4 cut(s) 158, 445, 547, 747
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 4 cut(s) 356, 551, 740, 804
HphI GGTGA 2 cut(s) 181, 719
Hpy188I TCNGA 2 cut(s) 94, 222
Hpy188III TCNNGA 8 cut(s) 56, 256, 370, 488, 505, 700, 737, 752
HpyAV CCTTC 3 cut(s) 439, 574, 821
HpyCH4III ACNGT 2 cut(s) 51, 409
HpyCH4IV ACGT 1 cut(s) 312
HpyCH4V TGCA 7 cut(s) 78, 154, 362, 477, 622, 686, 747
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 181
HpyF3I CTNAG 5 cut(s) 93, 134, 254, 515, 571
HpySE526I ACGT 1 cut(s) 312
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 4 cut(s) 158, 445, 547, 747
Ksp22I TGATCA 1 cut(s) 792
Kzo9I GATC 6 cut(s) 248, 258, 319, 461, 484, 792
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 9 cut(s) 241, 305, 385, 412, 487, 558, 583, 722, 737
Lsp1109I GCAGC 2 cut(s) 226, 670
LweI GCATC 2 cut(s) 226, 670
MaeI CTAG 1 cut(s) 834
MaeII ACGT 1 cut(s) 312
MaeIII GTNAC 3 cut(s) 532, 693, 707
MalI GATC 6 cut(s) 250, 260, 321, 463, 486, 794
MboI GATC 6 cut(s) 248, 258, 319, 461, 484, 792
MboII GAAGA 9 cut(s) 81, 102, 314, 351, 415, 456, 462, 659, 773
MfeI CAATTG 2 cut(s) 179, 788
MflI RGATCY 4 cut(s) 248, 258, 319, 461
MluCI AATT 4 cut(s) 60, 179, 665, 788
MlyI GAGTC 1 cut(s) 813
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 7 cut(s) 43, 88, 112, 195, 637, 667, 835
Mph1103I ATGCAT 1 cut(s) 749
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 1 cut(s) 729
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 597
MspR9I CCNGG 1 cut(s) 400
MunI CAATTG 2 cut(s) 179, 788
Mva1269I GAATGC 1 cut(s) 747
MvaI CCWGG 1 cut(s) 400
MwoI GCNNNNNNNGC 2 cut(s) 24, 181
NdeII GATC 6 cut(s) 248, 258, 319, 461, 484, 792
NlaIII CATG 4 cut(s) 158, 445, 547, 747
NmuCI GTSAC 3 cut(s) 532, 693, 707
NsiI ATGCAT 1 cut(s) 749
NspV TTCGAA 1 cut(s) 337
PceI AGGCCT 1 cut(s) 200
PcsI WCGNNNNNNNCGW 1 cut(s) 343
PctI GAATGC 1 cut(s) 747
PdmI GAANNNNTTC 1 cut(s) 555
PfeI GAWTC 3 cut(s) 356, 551, 740
PfoI TCCNGGA 1 cut(s) 398
PkrI GCNGC 2 cut(s) 216, 685
PleI GAGTC 1 cut(s) 812
PpsI GAGTC 1 cut(s) 812
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
PspPI GGNCC 1 cut(s) 575
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 4 cut(s) 248, 258, 319, 461
PvuII CAGCTG 1 cut(s) 597
RsaI GTAC 2 cut(s) 315, 366
RsaNI GTAC 2 cut(s) 314, 365
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 2 cut(s) 215, 684
Sau3AI GATC 6 cut(s) 248, 258, 319, 461, 484, 792
Sau96I GGNCC 1 cut(s) 575
ScaI AGTACT 1 cut(s) 366
SchI GAGTC 1 cut(s) 813
ScrFI CCNGG 1 cut(s) 400
SfaNI GCATC 2 cut(s) 226, 670
SfcI CTRYAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 3 cut(s) 34, 368, 698
SmoI CTYRAG 3 cut(s) 34, 368, 698
Sse9I AATT 4 cut(s) 60, 179, 665, 788
SseBI AGGCCT 1 cut(s) 200
SspMI CTAG 1 cut(s) 834
StuI AGGCCT 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 398
StyI CCWWGG 2 cut(s) 201, 303
TaaI ACNGT 2 cut(s) 51, 409
TaiI ACGT 1 cut(s) 315
TaqI TCGA 2 cut(s) 165, 337
TasI AATT 4 cut(s) 60, 179, 665, 788
TatI WGTACW 1 cut(s) 364
TfiI GAWTC 3 cut(s) 356, 551, 740
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 2 cut(s) 54, 414
TseFI GTSAC 3 cut(s) 532, 693, 707
TseI GCWGC 2 cut(s) 214, 683
Tsp45I GTSAC 3 cut(s) 532, 693, 707
TspDTI ATGAA 6 cut(s) 102, 311, 369, 458, 556, 678
TspRI CASTG 2 cut(s) 54, 414
XapI RAATTY 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 1 cut(s) 834
ZrmI AGTACT 1 cut(s) 366
Zsp2I ATGCAT 1 cut(s) 749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.