pycom17g21230

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000093
Physical Location & Seq
Forward (+)
19688496 .. 19691632
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g21230.9

Sequence Viewer

Length: 2538 bp
ATGCTTGCAGTGAAAGTGATTAACGTCTTGCTAAGTTTATACAATGAAATGAACGGAACAAAAATGAAATATAAGTATGTATGTATGTATAACAATTATGCGGTAAATGAGAATACGAACATGGACAATGGCAAAGTTTTAAAAGGCTGCCTTTGGTTGTCGAATCATAATAGCTTTGGTTGCGAAACCTTAGGCATTGTCCCTTCAGCTTGTTGGTCCCCTCCTGGCCCCTCTTTCATGAAATTGAATGTCGATGCGAGTTGGGTTGCGGGTTCTAAATGTGGCTATGCTGGAGTTGTGGTTAGGAATCATGAGGGAAGTTTTGTGGCTGCTAAAAGATTGCAGATTAATGCGCCAAATGTTTCGGCAGCGGAAGCTACTGCGATTTTGCTTGGTTATGATCTGGCTATTTCGTTGGGGTTGGACAGGATAATTGTTGAGTCCGACTCGAAGGAGAATATCTATAACTTAGTAAATGTTTCTTCTTCGGGCAGCTGGGAGGCTTTCCCCATCCTTACAAAAGCTTGGTGGCTGAGGGATTCCTTTCAGGACTGTCGCTGGTCTTGGGTTCCAAGATCGGCCAATATGGCAGCGCATCGGTTGGTGTTGAGTAGTCCGGAGATGTATTCTGAACAAAGATGGGTTACTCTGTCCCCCTTAATTTTTGTTTTGGCAACGGAGGGGCGACACCTTTGCCCTAGGTGCAGCGTCGTATGCTTTTGTATCCTCCTCTTCTGCTTGTTGTTTGATGCTTTCTTTGTAGCTTGCTTTCTGTGGGCTTCTTTGTTCGTTTGGCTTTGGCCCTTGTTCTATGATATTTCCAGTTCTTCAAAAAAAAAAAAAAAAAAAAAAAAAACCTTAGGCATTGATGTGATTGTAGATCGATCTAATTTGTATAAGGTTTCGCAATCATTTCATGTTTTCTTTTTCAAAAAAAAAAAAGAGGGTAAAAGACTGTTTACTACCCTCATATTTCGTGGTTTTCAACATTTAGTACATCAAGTTTTTTTCATCCTAGAATCATACCTAAAGTGTAAATTTTGGGACAATCTCATACATTCGTTAGTCAAACTGTTAAGTCTCCCGTTAACTGTGATTGTCAAAAAACCTTCATCTTCCCCAATTCCCACCCGTGCCCACCCTCCCATCACCCACCTCTTCGCCTTCCCCGCCCCCCCCCCCTCCCTTCCCCCCTCTTCAACCCCCCCCCTTCCCTCCCTTCTCTCTTCTGCACCTGCACCTGCACCACCACCCTCCCTTCTCTACTCTGCAACTCGTACCCACCCTCCCCGCACACCCAACCCTTCTCCCCCTGCGCCCCCAACCCAGGAGAACAAGAAGAACAAGAACCCAGAAGAAGAAGAAGGAAAAGAAAAAAACCTAGTTCGTCATTTCCAGATTAATGGAGATTTACCACAAAACCAGTCCAAAAATCACCTCAAACTCAAACTTCCCATCTCAAAGTATTTTCGAAATTCACCCACACTGAAAATCCCAAAAATATGGAGTTCATGGTCTGGGTTGCAGATTTATTTATTTATTTATCTTCTTCTTCTGGGTTGCAGATTCGATTTTTTTTCTTCTTCTTCTTCTTCTGGGTTGGGACCCGGGGGGGGGGGGGGAAGACTTATTCCTGCTCCTTCTTCTTCTTCTTCTGGTTTTTTATTTTTTCTCTTCTTCTTCTTCCTTCTTCTTTGGTTTTTCTTTTTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGGGTTTTTGGCCGTGGCGCGAGGGGGGGGTTGGGGGGGTGGGGAAGGCATGGGTTTTTTTATTTTTAACTTTTATTTAATTAATTATTTTAATATTTTTAATGACATGTGGCGTCCAGTCATTGTCCACATGGGAGCCACGTCATCACTTAACGGGAGAATTAACACTGCAACCCAGAAGAAGAAGAAGAAGAAGAGTGTTGCTATGAATGGTTGCTCTCGTGCGATCGACTTTCATACATTAATGCATGGTGAAATTGGCCTAAGGACTTACCAAGTCATTTGTTGTCACGCTCCGATCCAGACATATGGTCTACTTAACAACATTTACAATAACTCAGGTTCCAACAAGAAAGTCCAAAGGAAATATAAGAACTCCTCGTATCAACTAAGCTTTACCACCTGCACAATCACCCCTACATCGTGGGAATGGTGCACTGGCGATAATAATCTGATGTATGTGGTAATTATAGTGCAGTGGATCGAGACTTCGCGTGACATTGAACTCAACCTGTCTAGAGTGTGCAACCTCAACTCTGCAAGAAAGACTCCTTTTGTAAATATACTTATGAATGTTAACTCATCCTGTGCAAGTTGCTTGCCTATAGTATGTTTTCACGATCTTATAGTTATAAAATTTGTAAGCAACTTTAGTGCCCTTGCACTTGCCTTGCTCTTGTCCTCCAAAAAACCACTTCCAGCAGTCCATTTGGTGGAGGGCAATTGGTGGGCCAACACATGCCTGTCCAAAAGCTTTAGCAGCTTGAGCTCGTCCTTGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

846

Amino Acids

94.39

Weight (kDa)

9.54

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2387
AarI CACCTGC 3 cut(s) 1243, 1249, 2165
Acc36I ACCTGC 3 cut(s) 1243, 1249, 2165
AccB7I CCANNNNNTGG 1 cut(s) 2467
AccI GTMKAC 1 cut(s) 2068
AccII CGCG 2 cut(s) 1774, 2248
AccIII TCCGGA 1 cut(s) 616
AciI CCGC 5 cut(s) 101, 269, 371, 1170, 1291
AclWI GGATC 2 cut(s) 2047, 2243
AcoI YGGCCR 2 cut(s) 579, 1764
AcsI RAATTY 3 cut(s) 1037, 1474, 2390
AcuI CTGAAG 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 1867
AfaI GTAC 2 cut(s) 996, 1279
AfiI CCNNNNNNNGG 4 cut(s) 1327, 1613, 1614, 2467
AflIII ACRYGT 1 cut(s) 1860
AgsI TTSAA 6 cut(s) 247, 831, 931, 986, 1200, 2258
AhdI GACNNNNNGTC 1 cut(s) 2064
AjiI CACGTC 1 cut(s) 1896
AjnI CCWGG 2 cut(s) 223, 1326
Alw21I GWGCWC 2 cut(s) 2192, 2525
Alw26I GTCTC 2 cut(s) 1085, 2234
Alw44I GTGCAC 1 cut(s) 2188
AlwI GGATC 2 cut(s) 2047, 2243
Ama87I CYCGRG 1 cut(s) 1607
Aor13HI TCCGGA 1 cut(s) 616
AoxI GGCC 6 cut(s) 226, 579, 800, 1764, 2014, 2484
ApaLI GTGCAC 1 cut(s) 2188
ApeKI GCWGC 7 cut(s) 147, 329, 368, 492, 590, 705, 2514
ApoI RAATTY 3 cut(s) 1037, 1474, 2390
AseI ATTAAT 4 cut(s) 348, 1401, 1836, 1997
AspA2I CCTAGG 1 cut(s) 698
AspLEI GCGC 4 cut(s) 355, 595, 1318, 1774
AspS9I GGNCC 5 cut(s) 216, 227, 801, 1604, 2484
AsuC2I CCSGG 2 cut(s) 1608, 1609
AsuHPI GGTGA 5 cut(s) 1141, 1427, 1470, 2018, 2158
AsuII TTCGAA 1 cut(s) 1471
AvaI CYCGRG 1 cut(s) 1607
AvaII GGWCC 2 cut(s) 216, 1604
AvrII CCTAGG 1 cut(s) 698
AxyI CCTNAGG 3 cut(s) 190, 859, 2018
BaeGI GKGCMC 3 cut(s) 1138, 2192, 2413
BanII GRGCYC 1 cut(s) 2525
BauI CACGAG 1 cut(s) 1974
BbsI GAAGAC 1 cut(s) 1630
Bbv12I GWGCWC 2 cut(s) 2192, 2525
BbvCI CCTCAGC 1 cut(s) 533
BbvI GCAGC 7 cut(s) 134, 316, 380, 504, 602, 717, 2526
BccI CCATC 4 cut(s) 518, 633, 1154, 1463
BceAI ACGGC 1 cut(s) 1751
BcgI CGANNNNNNTGC 2 cut(s) 675, 709
BciT130I CCWGG 2 cut(s) 225, 1328
BciVI GTATCC 1 cut(s) 734
BcnI CCSGG 2 cut(s) 1608, 1609
BcoDI GTCTC 2 cut(s) 1085, 2234
BfaI CTAG 5 cut(s) 699, 1016, 1382, 2271, 2536
BfmI CTRYAG 1 cut(s) 2358
BfuAI ACCTGC 3 cut(s) 1243, 1249, 2165
BfuI GTATCC 1 cut(s) 734
BglI GCCNNNNNGGC 1 cut(s) 587
BisI GCNGC 7 cut(s) 148, 330, 369, 493, 591, 706, 2515
BlnI CCTAGG 1 cut(s) 698
BlsI GCNGC 7 cut(s) 149, 331, 370, 494, 592, 707, 2516
Bme1390I CCNGG 4 cut(s) 225, 1328, 1608, 1609
Bme18I GGWCC 2 cut(s) 216, 1604
BmeRI GACNNNNNGTC 1 cut(s) 2064
BmeT110I CYCGRG 1 cut(s) 1607
BmgBI CACGTC 1 cut(s) 1896
BmgT120I GGNCC 5 cut(s) 216, 227, 801, 1604, 2484
BmiI GGNNCC 7 cut(s) 218, 229, 570, 1605, 1606, 1891, 2098
BmrFI CCNGG 4 cut(s) 225, 1328, 1608, 1609
BmsI GCATC 3 cut(s) 244, 604, 739
BpiI GAAGAC 1 cut(s) 1630
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 533
Bpu14I TTCGAA 1 cut(s) 1471
BpuMI CCSGG 2 cut(s) 1608, 1609
Bsa29I ATCGAT 1 cut(s) 883
BsaBI GATNNNNATC 1 cut(s) 2202
BsaHI GRCGYC 1 cut(s) 1867
BsaJI CCNNGG 5 cut(s) 698, 1326, 1607, 1608, 1767
BsaWI WCCGGW 1 cut(s) 616
BsaXI ACNNNNNCTCC 4 cut(s) 285, 315, 1270, 1300
Bsc4I CCNNNNNNNGG 4 cut(s) 1327, 1613, 1614, 2467
Bse1I ACTGG 4 cut(s) 822, 1423, 1871, 2197
Bse21I CCTNAGG 3 cut(s) 190, 859, 2018
Bse8I GATNNNNATC 1 cut(s) 2202
BseAI TCCGGA 1 cut(s) 616
BseBI CCWGG 2 cut(s) 225, 1328
BseCI ATCGAT 1 cut(s) 883
BseDI CCNNGG 5 cut(s) 698, 1326, 1607, 1608, 1767
BseGI GGATG 3 cut(s) 510, 1011, 2336
BseJI GATNNNNATC 1 cut(s) 2202
BseLI CCNNNNNNNGG 4 cut(s) 1327, 1613, 1614, 2467
BseMII CTCAG 2 cut(s) 524, 2106
BseNI ACTGG 4 cut(s) 822, 1423, 1871, 2197
BseRI GAGGAG 2 cut(s) 719, 2122
BseSI GKGCMC 3 cut(s) 1138, 2192, 2413
BseXI GCAGC 7 cut(s) 134, 316, 380, 504, 602, 717, 2526
BseYI CCCAGC 1 cut(s) 495
BsgI GTGCAG 6 cut(s) 724, 1215, 1221, 1227, 2143, 2249
Bsh1236I CGCG 2 cut(s) 1774, 2248
Bsh1285I CGRYCG 1 cut(s) 1983
BshFI GGCC 6 cut(s) 228, 581, 802, 1766, 2016, 2486
BshVI ATCGAT 1 cut(s) 883
BsiEI CGRYCG 1 cut(s) 1983
BsiHKAI GWGCWC 2 cut(s) 2192, 2525
BsiHKCI CYCGRG 1 cut(s) 1607
BsiSI CCGG 2 cut(s) 617, 1608
BslFI GGGAC 5 cut(s) 185, 202, 637, 1058, 1617
BslI CCNNNNNNNGG 4 cut(s) 1327, 1613, 1614, 2467
BsmAI GTCTC 2 cut(s) 1085, 2234
BsmFI GGGAC 5 cut(s) 185, 202, 637, 1058, 1617
BsnI GGCC 6 cut(s) 228, 581, 802, 1766, 2016, 2486
BsoBI CYCGRG 1 cut(s) 1607
Bsp119I TTCGAA 1 cut(s) 1471
Bsp1286I GDGCHC 4 cut(s) 1138, 2192, 2413, 2525
Bsp13I TCCGGA 1 cut(s) 616
Bsp143I GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
BspACI CCGC 5 cut(s) 101, 269, 371, 1170, 1291
BspANI GGCC 6 cut(s) 228, 581, 802, 1766, 2016, 2486
BspCNI CTCAG 2 cut(s) 525, 2105
BspDI ATCGAT 1 cut(s) 883
BspEI TCCGGA 1 cut(s) 616
BspFNI CGCG 2 cut(s) 1774, 2248
BspHI TCATGA 2 cut(s) 237, 310
BspLI GGNNCC 7 cut(s) 218, 229, 570, 1605, 1606, 1891, 2098
BspMI ACCTGC 3 cut(s) 1243, 1249, 2165
BspPI GGATC 2 cut(s) 2047, 2243
BspT104I TTCGAA 1 cut(s) 1471
BsrI ACTGG 4 cut(s) 822, 1423, 1871, 2197
BssECI CCNNGG 5 cut(s) 698, 1326, 1607, 1608, 1767
BssMI GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
BssNI GRCGYC 1 cut(s) 1867
BssSI CACGAG 1 cut(s) 1974
BssT1I CCWWGG 1 cut(s) 698
Bst2BI CACGAG 1 cut(s) 1974
Bst2UI CCWGG 2 cut(s) 225, 1328
Bst4CI ACNGT 4 cut(s) 554, 957, 1074, 1093
Bst6I CTCTTC 7 cut(s) 737, 1163, 1201, 1231, 1679, 1717, 1943
BstACI GRCGYC 1 cut(s) 1867
BstBI TTCGAA 1 cut(s) 1471
BstC8I GCNNGC 3 cut(s) 6, 766, 2354
BstDEI CTNAG 8 cut(s) 32, 190, 469, 533, 859, 2018, 2092, 2144
BstDSI CCRYGG 1 cut(s) 1767
BstF5I GGATG 3 cut(s) 510, 1011, 2336
BstFNI CGCG 2 cut(s) 1774, 2248
BstHHI GCGC 4 cut(s) 355, 595, 1318, 1774
BstKTI GATC 8 cut(s) 403, 578, 883, 887, 1983, 2055, 2238, 2377
BstMAI GTCTC 2 cut(s) 1085, 2234
BstMBI GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
BstMCI CGRYCG 1 cut(s) 1983
BstMWI GCNNNNNNNGC 8 cut(s) 180, 374, 587, 702, 714, 1169, 2514, 2520
BstNI CCWGG 2 cut(s) 225, 1328
BstNSI RCATGY 2 cut(s) 1864, 2496
BstSCI CCNGG 4 cut(s) 223, 1326, 1606, 1607
BstSFI CTRYAG 1 cut(s) 2358
BstSLI GKGCMC 3 cut(s) 1138, 2192, 2413
BstUI CGCG 2 cut(s) 1774, 2248
BstV1I GCAGC 7 cut(s) 134, 316, 380, 504, 602, 717, 2526
BstV2I GAAGAC 1 cut(s) 1630
BstXI CCANNNNNNTGG 3 cut(s) 1403, 1503, 2063
Bsu15I ATCGAT 1 cut(s) 883
Bsu36I CCTNAGG 3 cut(s) 190, 859, 2018
BsuI GTATCC 1 cut(s) 734
BsuRI GGCC 6 cut(s) 228, 581, 802, 1766, 2016, 2486
BsuTUI ATCGAT 1 cut(s) 883
BtgI CCRYGG 1 cut(s) 1767
BtrI CACGTC 1 cut(s) 1896
BtsCI GGATG 3 cut(s) 510, 1011, 2336
BtsI GCAGTG 3 cut(s) 15, 1920, 2237
BtsIMutI CAGTG 5 cut(s) 15, 1484, 1920, 2190, 2237
BveI ACCTGC 3 cut(s) 1243, 1249, 2165
Cac8I GCNNGC 3 cut(s) 6, 766, 2354
CciI TCATGA 2 cut(s) 237, 310
CfoI GCGC 4 cut(s) 355, 595, 1318, 1774
Cfr13I GGNCC 5 cut(s) 216, 227, 801, 1604, 2484
Cfr9I CCCGGG 1 cut(s) 1607
ClaI ATCGAT 1 cut(s) 883
CseI GACGC 2 cut(s) 697, 1856
Csp6I GTAC 2 cut(s) 995, 1278
CviQI GTAC 2 cut(s) 995, 1278
DdeI CTNAG 8 cut(s) 32, 190, 469, 533, 859, 2018, 2092, 2144
DpnI GATC 8 cut(s) 402, 577, 882, 886, 1982, 2054, 2237, 2376
DpnII GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
DraI TTTAAA 1 cut(s) 141
DriI GACNNNNNGTC 1 cut(s) 2064
EaeI YGGCCR 2 cut(s) 579, 1764
Eam1104I CTCTTC 7 cut(s) 737, 1163, 1201, 1231, 1679, 1717, 1943
Eam1105I GACNNNNNGTC 1 cut(s) 2064
EarI CTCTTC 7 cut(s) 737, 1163, 1201, 1231, 1679, 1717, 1943
Ecl136II GAGCTC 1 cut(s) 2523
Eco130I CCWWGG 1 cut(s) 698
Eco24I GRGCYC 1 cut(s) 2525
Eco47I GGWCC 2 cut(s) 216, 1604
Eco53kI GAGCTC 1 cut(s) 2523
Eco57I CTGAAG 1 cut(s) 189
Eco81I CCTNAGG 3 cut(s) 190, 859, 2018
Eco88I CYCGRG 1 cut(s) 1607
EcoICRI GAGCTC 1 cut(s) 2523
EcoO109I RGGNCCY 1 cut(s) 1604
EcoRII CCWGG 2 cut(s) 223, 1326
EcoT14I CCWWGG 1 cut(s) 698
EcoT22I ATGCAT 1 cut(s) 2004
EcoT38I GRGCYC 1 cut(s) 2525
ErhI CCWWGG 1 cut(s) 698
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 5 cut(s) 185, 202, 637, 1058, 1617
FauI CCCGC 3 cut(s) 262, 1177, 1298
FauNDI CATATG 1 cut(s) 2062
FblI GTMKAC 1 cut(s) 2068
Fnu4HI GCNGC 7 cut(s) 148, 330, 369, 493, 591, 706, 2515
FokI GGATG 3 cut(s) 497, 998, 2323
FriOI GRGCYC 1 cut(s) 2525
Fsp4HI GCNGC 7 cut(s) 148, 330, 369, 493, 591, 706, 2515
FspBI CTAG 5 cut(s) 699, 1016, 1382, 2271, 2536
GlaI GCGC 4 cut(s) 354, 594, 1317, 1773
GluI GCNGC 7 cut(s) 148, 330, 369, 493, 591, 706, 2515
GsaI CCCAGC 1 cut(s) 499
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 6 cut(s) 228, 581, 802, 1766, 2016, 2486
HapII CCGG 2 cut(s) 617, 1608
HgaI GACGC 2 cut(s) 697, 1856
HhaI GCGC 4 cut(s) 355, 595, 1318, 1774
Hin1I GRCGYC 1 cut(s) 1867
Hin6I GCGC 4 cut(s) 353, 593, 1316, 1772
HinP1I GCGC 4 cut(s) 353, 593, 1316, 1772
HincII GTYRAC 2 cut(s) 1089, 2332
HindII GTYRAC 2 cut(s) 1089, 2332
HindIII AAGCTT 3 cut(s) 522, 2146, 2506
HinfI GANTC 8 cut(s) 163, 307, 440, 446, 539, 1019, 1566, 2302
HpaI GTTAAC 2 cut(s) 1089, 2332
HpaII CCGG 2 cut(s) 617, 1608
HphI GGTGA 5 cut(s) 1141, 1427, 1470, 2018, 2158
Hpy166II GTNNAC 6 cut(s) 960, 1089, 1882, 2069, 2190, 2332
Hpy188I TCNGA 4 cut(s) 445, 631, 2052, 2208
Hpy188III TCNNGA 9 cut(s) 238, 311, 548, 617, 1396, 2056, 2239, 2271, 2372
Hpy8I GTNNAC 6 cut(s) 960, 1089, 1882, 2069, 2190, 2332
Hpy99I CGWCG 1 cut(s) 713
HpyCH4III ACNGT 4 cut(s) 554, 957, 1074, 1093
HpyCH4IV ACGT 2 cut(s) 24, 1895
HpyF10VI GCNNNNNNNGC 8 cut(s) 180, 374, 587, 702, 714, 1169, 2514, 2520
HpyF3I CTNAG 8 cut(s) 32, 190, 469, 533, 859, 2018, 2092, 2144
HpySE526I ACGT 2 cut(s) 24, 1895
Hsp92I GRCGYC 1 cut(s) 1867
HspAI GCGC 4 cut(s) 353, 593, 1316, 1772
KflI GGGWCCC 1 cut(s) 1604
Kpn2I TCCGGA 1 cut(s) 616
KspAI GTTAAC 2 cut(s) 1089, 2332
Kzo9I GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
LmnI GCTCC 3 cut(s) 1642, 1889, 2053
Lsp1109I GCAGC 7 cut(s) 134, 316, 380, 504, 602, 717, 2526
LweI GCATC 3 cut(s) 244, 604, 739
MaeI CTAG 5 cut(s) 699, 1016, 1382, 2271, 2536
MaeII ACGT 2 cut(s) 24, 1895
MaeIII GTNAC 3 cut(s) 643, 2042, 2249
MalI GATC 8 cut(s) 402, 577, 882, 886, 1982, 2054, 2237, 2376
MboI GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
MfeI CAATTG 1 cut(s) 2476
MhlI GDGCHC 4 cut(s) 1138, 2192, 2413, 2525
MlyI GAGTC 3 cut(s) 440, 449, 2296
MmeI TCCRAC 3 cut(s) 402, 468, 2124
Mph1103I ATGCAT 1 cut(s) 2004
MroI TCCGGA 1 cut(s) 616
MslI CAYNNNNRTG 1 cut(s) 869
MspA1I CMGCKG 2 cut(s) 371, 495
MspI CCGG 2 cut(s) 617, 1608
MspR9I CCNGG 4 cut(s) 225, 1328, 1608, 1609
MunI CAATTG 1 cut(s) 2476
MvaI CCWGG 2 cut(s) 225, 1328
MvnI CGCG 2 cut(s) 1774, 2248
MwoI GCNNNNNNNGC 8 cut(s) 180, 374, 587, 702, 714, 1169, 2514, 2520
NciI CCSGG 2 cut(s) 1608, 1609
NdeI CATATG 1 cut(s) 2062
NdeII GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
NlaIV GGNNCC 7 cut(s) 218, 229, 570, 1605, 1606, 1891, 2098
NmuCI GTSAC 2 cut(s) 2042, 2249
NsiI ATGCAT 1 cut(s) 2004
NspI RCATGY 2 cut(s) 1864, 2496
NspV TTCGAA 1 cut(s) 1471
PacI TTAATTAA 1 cut(s) 1836
PagI TCATGA 2 cut(s) 237, 310
PaqCI CACCTGC 3 cut(s) 1243, 1249, 2165
PciI ACATGT 1 cut(s) 1860
PfeI GAWTC 5 cut(s) 163, 307, 539, 1019, 1566
PflMI CCANNNNNTGG 1 cut(s) 2467
PkrI GCNGC 7 cut(s) 149, 331, 370, 494, 592, 707, 2516
Ple19I CGATCG 1 cut(s) 1983
PleI GAGTC 3 cut(s) 440, 448, 2296
PpsI GAGTC 3 cut(s) 440, 448, 2296
PpuMI RGGWCCY 1 cut(s) 1604
PscI ACATGT 1 cut(s) 1860
PshBI ATTAAT 4 cut(s) 348, 1401, 1836, 1997
PsiI TTATAA 1 cut(s) 2387
Psp124BI GAGCTC 1 cut(s) 2525
Psp5II RGGWCCY 1 cut(s) 1604
Psp6I CCWGG 2 cut(s) 223, 1326
PspFI CCCAGC 1 cut(s) 495
PspGI CCWGG 2 cut(s) 223, 1326
PspN4I GGNNCC 7 cut(s) 218, 229, 570, 1605, 1606, 1891, 2098
PspPI GGNCC 5 cut(s) 216, 227, 801, 1604, 2484
PspPPI RGGWCCY 1 cut(s) 1604
PvuI CGATCG 1 cut(s) 1983
PvuII CAGCTG 1 cut(s) 495
RsaI GTAC 2 cut(s) 996, 1279
RsaNI GTAC 2 cut(s) 995, 1278
RseI CAYNNNNRTG 1 cut(s) 869
SacI GAGCTC 1 cut(s) 2525
SatI GCNGC 7 cut(s) 148, 330, 369, 493, 591, 706, 2515
Sau3AI GATC 8 cut(s) 400, 575, 880, 884, 1980, 2052, 2235, 2374
Sau96I GGNCC 5 cut(s) 216, 227, 801, 1604, 2484
SchI GAGTC 3 cut(s) 440, 449, 2296
ScrFI CCNGG 4 cut(s) 225, 1328, 1608, 1609
SduI GDGCHC 4 cut(s) 1138, 2192, 2413, 2525
SfaNI GCATC 3 cut(s) 244, 604, 739
SfcI CTRYAG 1 cut(s) 2358
SfuI TTCGAA 1 cut(s) 1471
SinI GGWCC 2 cut(s) 216, 1604
SmaI CCCGGG 1 cut(s) 1609
SmiMI CAYNNNNRTG 1 cut(s) 869
SmlI CTYRAG 1 cut(s) 2518
SmoI CTYRAG 1 cut(s) 2518
SsiI CCGC 5 cut(s) 101, 269, 371, 1170, 1291
SspI AATATT 1 cut(s) 1849
SspMI CTAG 5 cut(s) 699, 1016, 1382, 2271, 2536
SstI GAGCTC 1 cut(s) 2525
StyD4I CCNGG 4 cut(s) 223, 1326, 1606, 1607
StyI CCWWGG 1 cut(s) 698
TaaI ACNGT 4 cut(s) 554, 957, 1074, 1093
TaiI ACGT 2 cut(s) 27, 1898
TaqI TCGA 8 cut(s) 161, 252, 449, 883, 1471, 1569, 1983, 2238
TatI WGTACW 1 cut(s) 994
TfiI GAWTC 5 cut(s) 163, 307, 539, 1019, 1566
TscAI CASTG 5 cut(s) 15, 1491, 1927, 2197, 2237
TseFI GTSAC 2 cut(s) 2042, 2249
TseI GCWGC 7 cut(s) 147, 329, 368, 492, 590, 705, 2514
Tsp45I GTSAC 2 cut(s) 2042, 2249
TspGWI ACGGA 2 cut(s) 69, 692
TspMI CCCGGG 1 cut(s) 1607
TspRI CASTG 5 cut(s) 15, 1491, 1927, 2197, 2237
Van91I CCANNNNNTGG 1 cut(s) 2467
VneI GTGCAC 1 cut(s) 2188
VpaK11BI GGWCC 2 cut(s) 216, 1604
VspI ATTAAT 4 cut(s) 348, 1401, 1836, 1997
XapI RAATTY 3 cut(s) 1037, 1474, 2390
XbaI TCTAGA 1 cut(s) 2270
XceI RCATGY 2 cut(s) 1864, 2496
XmaI CCCGGG 1 cut(s) 1607
XmaJI CCTAGG 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 2068
XspI CTAG 5 cut(s) 699, 1016, 1382, 2271, 2536
Zsp2I ATGCAT 1 cut(s) 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.