Rmu_sc0000654.1_g000022

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000654.1
OG0000093
Physical Location & Seq
Forward (+)
108983 .. 113100
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000654.1_g000022.1.cds

Sequence Viewer

Length: 741 bp
atgaaggtcaaacagaagtcgcagagattggaggtgtttcagcaattcattgatcagcacccacgggtcggcgaggacgaggtggtcagggcaagcgactgtgacggtctccacgggcaagttttttgctccgggagagccctcgttgagacttcgagagtgagacggtgggtatcgcgggttcgaagaagaagggcagcagcaatgggttatgaacgattatggataatagggctgggcacggtcccaaaccggcaccacatttatagggaccgggaccggaccggcagagttaagttcgggctgggcttgcatatgacagacacatggaccggcctcaacccggaccacgacagaaatgggccggtttcttcgggctttcggttcctggcatatttttccgcacctgttgaacaatcctcatcatatgctactagacatgaaccccaccttagatggcagccccccaatcccccttatgcaaaagttaatgtagctagagcttggaatgaagaaagtcaacacagtgggattggaattattatcagaaaccatagaggtcactctattgctggtgcaagcattcatgggagtcacaactctgccatcgaagtagatgctgaggccgtggttaagggcctccagctcgctgctcaattgaatcttcagaagataattgttgaaggggattgcaaagaagtcttaacagcaaacaacaattcatctttctccccaaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

246

Amino Acids

27.68

Weight (kDa)

9.83

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000093)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G09490
fragaria_vesca FvH4_1g23791 FvH4_2g01641 FvH4_2g04612 FvH4_2g06831 FvH4_2g07841 FvH4_2g13581 FvH4_2g27220 FvH4_2g30621 FvH4_2g34753 FvH4_3g10392 FvH4_3g24291 FvH4_3g25030 FvH4_3g29361 FvH4_3g31372 FvH4_3g32402 FvH4_3g43651 FvH4_4g03830 FvH4_4g05481 FvH4_4g20431 FvH4_4g21421 FvH4_4g25931 FvH4_5g08801 FvH4_5g21021 FvH4_5g26254 FvH4_5g30931 FvH4_5g31281 FvH4_5g33581 FvH4_5g35622 FvH4_6g02100 FvH4_6g07963 FvH4_6g11620 FvH4_6g16031 FvH4_6g19041 FvH4_6g24211 FvH4_6g24271 FvH4_6g30670 FvH4_6g49540 FvH4_7g25914
malus_domestica MD03G1245200.v1.1 MD04G1168000.v1.1 MD09G1242600.v1.1 MD15G1334000.v1.1 MD17G1165700.v1.1
prunus_persica Prupe.1G073500_v2.0.a1 Prupe.1G283700_v2.0.a1 Prupe.1G524100_v2.0.a1 Prupe.2G022700_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G022800_v2.0.a1 Prupe.2G044300_v2.0.a1 Prupe.2G048800_v2.0.a1 Prupe.2G092000_v2.0.a1 Prupe.2G216500_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.2G216600_v2.0.a1 Prupe.3G103400_v2.0.a1 Prupe.3G143300_v2.0.a1 Prupe.3G190000_v2.0.a1 Prupe.3G219800_v2.0.a1 Prupe.4G177800_v2.0.a1 Prupe.4G261300_v2.0.a1 Prupe.5G044600_v2.0.a1 Prupe.5G067400_v2.0.a1 Prupe.7G083900_v2.0.a1 Prupe.7G120900_v2.0.a1 Prupe.8G032200_v2.0.a1 Prupe.8G036100_v2.0.a1 Prupe.8G042700_v2.0.a1
pyrus_communis pycom01g21710 pycom02g19770 pycom03g05120 pycom05g01370 pycom05g08780 pycom05g10120 pycom07g01370 pycom07g05930 pycom08g15760 pycom08g18090 pycom08g21060 pycom09g03880 pycom10g03870 pycom13g22240 pycom16g05120 pycom16g17170 pycom17g12180 pycom17g16290 pycom17g21230 pycom17g24990 pycom215g00100
rosa_chinensis RchiOBHm_Chr1g0323031 RchiOBHm_Chr1g0324391 RchiOBHm_Chr1g0326401 RchiOBHm_Chr1g0327811 RchiOBHm_Chr1g0332521 RchiOBHm_Chr1g0336501 RchiOBHm_Chr1g0346021 RchiOBHm_Chr1g0347191 RchiOBHm_Chr1g0367181 RchiOBHm_Chr2g0089441 RchiOBHm_Chr2g0115441 RchiOBHm_Chr3g0490691 RchiOBHm_Chr4g0395251 RchiOBHm_Chr4g0398081 RchiOBHm_Chr4g0409451 RchiOBHm_Chr4g0413041 RchiOBHm_Chr4g0415471 RchiOBHm_Chr4g0420961 RchiOBHm_Chr5g0040301 RchiOBHm_Chr5g0070651 RchiOBHm_Chr6g0263011 RchiOBHm_Chr6g0270721 RchiOBHm_Chr6g0277111 RchiOBHm_Chr7g0179771 RchiOBHm_Chr7g0223051 RchiOBHm_Chr7g0225031 RchiOBHm_Chr7g0225091
rosa_laevigata RLG00000001668 RLG00000001672 RLG00000001676 RLG00000007762 RLG00000008994 RLG00000011117 RLG00000013729 RLG00000013839 RLG00000014759 RLG00000016638 RLG00000018535 RLG00000018614 RLG00000018646 RLG00000020679 RLG00000023337 RLG00000029348 RLG00000035891 RLG00000035912
rosa_multiflora Rmu_co8152500.1_g000001 Rmu_co8203314.1_g000001 Rmu_co8383183.1_g000001 Rmu_sc0000293.1_g000033 Rmu_sc0000367.1_g000226 Rmu_sc0000512.1_g000023 Rmu_sc0000594.1_g000059 Rmu_sc0000654.1_g000022 Rmu_sc0000654.1_g000047 Rmu_sc0000706.1_g000037 Rmu_sc0001348.1_g000008 Rmu_sc0001385.1_g000024 Rmu_sc0001405.1_g000002 Rmu_sc0001521.1_g000048 Rmu_sc0001932.1_g000008 Rmu_sc0001932.1_g000009 Rmu_sc0002535.1_g000011 Rmu_sc0002908.1_g000019 Rmu_sc0003709.1_g000004 Rmu_sc0003882.1_g000054 Rmu_sc0004035.1_g000007 Rmu_sc0004041.1_g000007 Rmu_sc0004288.1_g000011 Rmu_sc0004569.1_g000004 Rmu_sc0004677.1_g000016 Rmu_sc0004834.1_g000010 Rmu_sc0005193.1_g000008 Rmu_sc0005354.1_g000009 Rmu_sc0006514.1_g000021 Rmu_sc0006691.1_g000001 Rmu_sc0006888.1_g000021 Rmu_sc0006889.1_g000013 Rmu_sc0008096.1_g000017 Rmu_sc0008378.1_g000003 Rmu_sc0009127.1_g000005 Rmu_sc0009191.1_g000016 Rmu_sc0009687.1_g000018 Rmu_sc0009709.1_g000005 Rmu_sc0009880.1_g000015 Rmu_sc0011710.1_g000001 Rmu_sc0012615.1_g000004 Rmu_sc0016091.1_g000002 Rmu_sc0022339.1_g000006 Rmu_sc0026537.1_g000002 Rmu_sc0027689.1_g000002 Rmu_sc0036222.1_g000001 Rmu_sc0040890.1_g000001 Rmu_ssc0000148.1_g000006 Rmu_ssc0000151.1_g000017 Rmu_ssc0000404.1_g000018 Rmu_ssc0000449.1_g000009
rosa_roxburghii Rroxscaffold_1G00017760 Rroxscaffold_1G00059150 Rroxscaffold_2G00142560 Rroxscaffold_3G00220440 Rroxscaffold_3G00231700 Rroxscaffold_3G00232400 Rroxscaffold_3G00274510 Rroxscaffold_4G00288790 Rroxscaffold_4G00305680 Rroxscaffold_4G00314880 Rroxscaffold_4G00314900 Rroxscaffold_5G00334590 Rroxscaffold_6G00394110 Rroxscaffold_6G00415860 Rroxscaffold_7G00168240 Rroxscaffold_7G00204400 Rroxscaffold_7G00207840
rosa_rugosa Rorug01G0034900 Rorug02G0079500 Rorug02G0229000 Rorug02G0229100 Rorug02G0291400 Rorug02G0298800 Rorug03G0046400 Rorug03G0064700 Rorug03G0077300 Rorug03G0199000 Rorug03G0200400 Rorug04G0205200 Rorug05G0025400 Rorug05G0127400 Rorug05G0192100 Rorug05G0453400 Rorug05G0538300 Rorug06G0035200 Rorug06G0241000 Rorug06G0268300 Rorug07G0120300 Rorug07G0145000 Rorug07G0209100 Rorug07G0240500 Rorug07G0271700
rosa_samantha Rh1AG179100 Rh1BG106900 Rh1BG163400 Rh1BG163500 Rh1BG323500 Rh2CG447900 Rh2DG503700 Rh3AG293600 Rh3CG229100 Rh3DG221500 Rh4AG106000 Rh4BG267700 Rh4CG062500 Rh4CG090100 Rh4CG114800 Rh5AG038400 Rh5BG395400 Rh5CG441700 Rh5CG572000 Rh6AG222400 Rh6AG264500 Rh6BG137400 Rh6BG225700 Rh6CG144300 Rh6DG087600 Rh6DG096000 Rh6DG362400 Rh6DG390200 Rh7AG207500 Rh7AG324700 Rh7AG388800 Rh7AG437100 Rh7BG019000 Rh7BG374600 Rh7CG028700 Rh7CG341500 Rh7CG408000 Rh7CG408300 Rh7DG354600
rosa_wichuraiana Rw0G009630 Rw0G012170 Rw4G022700 Rw5G028420 Rw5G029490 Rw6G007220 Rw6G007410 Rw6G017880 Rw7G012440 Rw7G021830 Rw7G027490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AccB1I GGYRCC 1 cut(s) 255
AccII CGCG 1 cut(s) 178
AciI CCGC 2 cut(s) 178, 402
AcuI CTGAAG 1 cut(s) 650
AfiI CCNNNNNNNGG 2 cut(s) 68, 343
AgsI TTSAA 3 cut(s) 413, 661, 683
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 3 cut(s) 497, 503, 646
AluI AGCT 3 cut(s) 497, 503, 646
Alw26I GTCTC 3 cut(s) 113, 143, 157
AoxI GGCC 4 cut(s) 334, 362, 624, 637
ApeKI GCWGC 4 cut(s) 197, 200, 460, 650
AspS9I GGNCC 8 cut(s) 244, 271, 277, 282, 330, 346, 362, 637
AsuC2I CCSGG 3 cut(s) 133, 275, 344
AsuII TTCGAA 1 cut(s) 184
AvaII GGWCC 6 cut(s) 244, 271, 277, 282, 330, 346
BaeGI GKGCMC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 142
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 4 cut(s) 209, 212, 472, 637
BccI CCATC 2 cut(s) 450, 614
BceAI ACGGC 1 cut(s) 611
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 1 cut(s) 389
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 3 cut(s) 133, 275, 344
BcoDI GTCTC 3 cut(s) 113, 143, 157
BfaI CTAG 2 cut(s) 435, 498
BisI GCNGC 4 cut(s) 198, 201, 461, 651
BlsI GCNGC 4 cut(s) 199, 202, 462, 652
Bme1390I CCNGG 4 cut(s) 133, 275, 344, 389
Bme18I GGWCC 6 cut(s) 244, 271, 277, 282, 330, 346
BmgT120I GGNCC 8 cut(s) 244, 271, 277, 282, 330, 346, 362, 637
BmiI GGNNCC 5 cut(s) 246, 257, 272, 278, 386
BmrFI CCNGG 4 cut(s) 133, 275, 344, 389
BmsI GCATC 1 cut(s) 607
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 184
BpuMI CCSGG 3 cut(s) 133, 275, 344
BsaI GGTCTC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 62, 112, 627
BsaWI WCCGGW 1 cut(s) 279
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 343
Bse118I RCCGGY 4 cut(s) 252, 284, 332, 364
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 389
BseDI CCNNGG 3 cut(s) 62, 112, 627
BseLI CCNNNNNNNGG 2 cut(s) 68, 343
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 1 cut(s) 612
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 4 cut(s) 209, 212, 472, 637
BseYI CCCAGC 2 cut(s) 235, 304
Bsh1236I CGCG 1 cut(s) 178
BshFI GGCC 4 cut(s) 336, 364, 626, 639
BshNI GGYRCC 1 cut(s) 255
BsiSI CCGG 8 cut(s) 132, 253, 274, 280, 285, 333, 344, 365
BslFI GGGAC 3 cut(s) 230, 284, 290
BslI CCNNNNNNNGG 2 cut(s) 68, 343
BsmAI GTCTC 3 cut(s) 113, 143, 157
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 3 cut(s) 230, 284, 290
BsmI GAATGC 1 cut(s) 582
BsnI GGCC 4 cut(s) 336, 364, 626, 639
Bso31I GGTCTC 1 cut(s) 113
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 142, 242
Bsp143I GATC 1 cut(s) 52
BspACI CCGC 2 cut(s) 178, 402
BspANI GGCC 4 cut(s) 336, 364, 626, 639
BspCNI CTCAG 1 cut(s) 613
BspFNI CGCG 1 cut(s) 178
BspLI GGNNCC 5 cut(s) 246, 257, 272, 278, 386
BspT104I TTCGAA 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 255
BspTNI GGTCTC 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 4 cut(s) 252, 284, 332, 364
BssAI RCCGGY 4 cut(s) 252, 284, 332, 364
BssECI CCNNGG 3 cut(s) 62, 112, 627
BssMI GATC 1 cut(s) 52
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 5 cut(s) 101, 107, 168, 244, 527
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 4 cut(s) 94, 311, 580, 648
BstDEI CTNAG 2 cut(s) 452, 621
BstDSI CCRYGG 3 cut(s) 62, 112, 627
BstFNI CGCG 1 cut(s) 178
BstKTI GATC 1 cut(s) 55
BstMAI GTCTC 3 cut(s) 113, 143, 157
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 4 cut(s) 131, 273, 342, 387
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 1 cut(s) 178
BstV1I GCAGC 4 cut(s) 209, 212, 472, 637
BsuRI GGCC 4 cut(s) 336, 364, 626, 639
BtgI CCRYGG 3 cut(s) 62, 112, 627
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 4 cut(s) 94, 311, 580, 648
Cfr10I RCCGGY 4 cut(s) 252, 284, 332, 364
Cfr13I GGNCC 8 cut(s) 244, 271, 277, 282, 330, 346, 362, 637
CpoI CGGWCCG 1 cut(s) 282
CspI CGGWCCG 1 cut(s) 282
CviAII CATG 3 cut(s) 327, 440, 587
DdeI CTNAG 2 cut(s) 452, 621
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
Eco24I GRGCYC 1 cut(s) 142
Eco31I GGTCTC 1 cut(s) 113
Eco47I GGWCC 6 cut(s) 244, 271, 277, 282, 330, 346
Eco57I CTGAAG 1 cut(s) 650
EcoO109I RGGNCCY 1 cut(s) 637
EcoRII CCWGG 1 cut(s) 387
EcoT38I GRGCYC 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 3 cut(s) 330, 443, 590
FaqI GGGAC 3 cut(s) 230, 284, 290
FatI CATG 3 cut(s) 326, 439, 586
FauI CCCGC 1 cut(s) 171
FauNDI CATATG 2 cut(s) 315, 427
FbaI TGATCA 1 cut(s) 52
Fnu4HI GCNGC 4 cut(s) 198, 201, 461, 651
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 4 cut(s) 198, 201, 461, 651
FspBI CTAG 2 cut(s) 435, 498
GluI GCNGC 4 cut(s) 198, 201, 461, 651
GsaI CCCAGC 2 cut(s) 239, 308
GsuI CTGGAG 1 cut(s) 626
HaeIII GGCC 4 cut(s) 336, 364, 626, 639
HapII CCGG 8 cut(s) 132, 253, 274, 280, 285, 333, 344, 365
Hin1II CATG 3 cut(s) 330, 443, 590
HincII GTYRAC 1 cut(s) 521
HindII GTYRAC 1 cut(s) 521
HinfI GANTC 2 cut(s) 592, 661
HpaII CCGG 8 cut(s) 132, 253, 274, 280, 285, 333, 344, 365
Hpy166II GTNNAC 1 cut(s) 521
Hpy188I TCNGA 2 cut(s) 548, 669
Hpy188III TCNNGA 1 cut(s) 156
Hpy8I GTNNAC 1 cut(s) 521
HpyAV CCTTC 2 cut(s) 186, 677
HpyCH4III ACNGT 5 cut(s) 101, 107, 168, 244, 527
HpyCH4V TGCA 4 cut(s) 313, 482, 578, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 452, 621
Hsp92II CATG 3 cut(s) 330, 443, 590
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 134
Lsp1109I GCAGC 4 cut(s) 209, 212, 472, 637
LweI GCATC 1 cut(s) 607
MaeI CTAG 2 cut(s) 435, 498
MaeIII GTNAC 3 cut(s) 101, 560, 593
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 6 cut(s) 198, 201, 363, 524, 656, 682
MfeI CAATTG 1 cut(s) 656
MhlI GDGCHC 2 cut(s) 142, 242
MluCI AATT 5 cut(s) 44, 537, 656, 675, 718
MlyI GAGTC 1 cut(s) 601
MnlI CCTC 9 cut(s) 25, 67, 73, 152, 347, 430, 551, 616, 650
MseI TTAA 4 cut(s) 294, 489, 633, 704
MspI CCGG 8 cut(s) 132, 253, 274, 280, 285, 333, 344, 365
MspR9I CCNGG 4 cut(s) 133, 275, 344, 389
MunI CAATTG 1 cut(s) 656
Mva1269I GAATGC 1 cut(s) 582
MvaI CCWGG 1 cut(s) 389
MvnI CGCG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 310
NciI CCSGG 3 cut(s) 133, 275, 344
NdeI CATATG 2 cut(s) 315, 427
NdeII GATC 1 cut(s) 52
NlaIII CATG 3 cut(s) 330, 443, 590
NlaIV GGNNCC 5 cut(s) 246, 257, 272, 278, 386
NmuCI GTSAC 3 cut(s) 101, 560, 593
NspV TTCGAA 1 cut(s) 184
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 582
PfeI GAWTC 1 cut(s) 661
PfoI TCCNGGA 1 cut(s) 131
PkrI GCNGC 4 cut(s) 199, 202, 462, 652
PleI GAGTC 1 cut(s) 600
PpsI GAGTC 1 cut(s) 600
Psp6I CCWGG 1 cut(s) 387
PspFI CCCAGC 2 cut(s) 235, 304
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 5 cut(s) 246, 257, 272, 278, 386
PspPI GGNCC 8 cut(s) 244, 271, 277, 282, 330, 346, 362, 637
PsrI GAACNNNNNNTAC 2 cut(s) 165, 197
Rsr2I CGGWCCG 1 cut(s) 282
RsrII CGGWCCG 1 cut(s) 282
SaqAI TTAA 4 cut(s) 294, 489, 633, 704
SatI GCNGC 4 cut(s) 198, 201, 461, 651
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 8 cut(s) 244, 271, 277, 282, 330, 346, 362, 637
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 4 cut(s) 133, 275, 344, 389
SduI GDGCHC 2 cut(s) 142, 242
SetI ASST 9 cut(s) 9, 36, 84, 409, 453, 499, 505, 562, 648
SfaNI GCATC 1 cut(s) 607
SfuI TTCGAA 1 cut(s) 184
SinI GGWCC 6 cut(s) 244, 271, 277, 282, 330, 346
Sse9I AATT 5 cut(s) 44, 537, 656, 675, 718
SsiI CCGC 2 cut(s) 178, 402
SspMI CTAG 2 cut(s) 435, 498
StyD4I CCNGG 4 cut(s) 131, 273, 342, 387
TaaI ACNGT 5 cut(s) 101, 107, 168, 244, 527
TaqI TCGA 3 cut(s) 155, 184, 609
TasI AATT 5 cut(s) 44, 537, 656, 675, 718
TfiI GAWTC 1 cut(s) 661
Tru1I TTAA 4 cut(s) 294, 489, 633, 704
Tru9I TTAA 4 cut(s) 294, 489, 633, 704
TscAI CASTG 1 cut(s) 532
TseFI GTSAC 3 cut(s) 101, 560, 593
TseI GCWGC 4 cut(s) 197, 200, 460, 650
Tsp45I GTSAC 3 cut(s) 101, 560, 593
TspDTI ATGAA 7 cut(s) 17, 37, 228, 456, 525, 575, 711
TspRI CASTG 1 cut(s) 532
VpaK11BI GGWCC 6 cut(s) 244, 271, 277, 282, 330, 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 356
XspI CTAG 2 cut(s) 435, 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.