FvH4_3g41030
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000088
Physical Location & Seq
Forward (+)
34523755 .. 34542701
18947 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41030.t9

Sequence Viewer

Length: 3279 bp
ATGTCATTGGTGGTCTTGTTTTGGTATGCTGCAGCAGCCTGCAGCATATTCATCTCTGGGTTTGATGTACAAGCTCAACCCACCACAGATCCTTCTGAAGTGAGAGCGCTAAATGCAATCTTTCAGAAGTGGAATATTACAAACAAAGTTAAGCAATGGAACACGACTGGTGACCCGTGCAGTGGAATTGCGATAGATGAAGATGATGACACCAGTTATTTCTATGATGCAACTAATTACAATCCGTTCGTCAAATGCAACTGTTCTTATGATTCAAACACAACTTGCCACATTACCCAACTGAAAGCATCTATATTGGACATTGTTGCTCCAATTCCAGAAGAGCTCTATACTTTGACTTTTCTTCAATATCTGGATGTGAAAAAAAATTACTTGACAGGACCTATCTCTGCATCAATTGGAAATCTAAGCAGCGTCACGTACTTGAACTTGGGACACAACGACTTATCAGGTGAATTTCCAAAGGACATTGTAAAACTCAACAATTTGACATACTTGAACTTGGGACACAACGACTTATCAGGTGAATTACCAAAGGACATTGTAAAACTCAACAATTTGACATACTTGGCTCTTGGGACACTCGACCTTTCTGGTTCTCTGCCACCTGAGATAGGGTACATAACAACACTAATAAATATTTTCATAGATAATTCCGGACTTAGCGGTGAGATTCCACCAACATTTGCTAATCTAACAAACTTGGAGCAATTTTGGGCAACAGATACAAACCTCACTGGCAGGCTACCAGACTTCGTAGGAAATTGGACAAAGCTTACTACCTTGAGGTTTCAGGGAAACTCTTTTGAAGGTTCTATACCATCTTCACTGTCCAGATTGACTCGTTTGACAGAGCTGCGAATTGCTGATTTAACGATTGACAATGACAACTCTTCTCTTGGATTTATCAAGAATATGACGTCTCTAGAAGTCTTAATGCTGAGGAATAACAATATTACTGATTCAATTCCTTCCAACATTGGAGATTTCCTAAAATTGGTTCACCTGGATTTGAGCTTCAACAATTTGGTTGGACAAATACCAGCCTCACTATTTAATATGAATGTGCTCGCCATCCTGTTTCTTGGAAACAACAAGTTGAATGGTAGCCTTCCTGTGTCTAAAAGCTCAGCTCTTCTTGTTGTAGATTTGTCTTACAATAATCTAATAGGCCAGAGCTTTCCAAGTTGGGTCAGCGAACAAAACTTACAGCTTAACTTAGTGGCCAACAACTTCAGCATAGACAGTTCAGATAGCAGTGGTCTACCTTCAGGGTTGAATTGCCTTCAAAGGGGCTTTCCTTGCCACCGTGATAGTCCAGTTTATTATAACTTCATGATTAATTGCGGTGGTCCGCAGACTAAGATATCCAATGGGACTGTGTATGAGCAGGACAGTGAGCCCCTTGGTACAGCTACATATTATGTGACTGAAACAAACAGATGGGGAGTTAGCAATGTCGGTCTTAAAACACAGCTTGTGGCTGCATCATATTTGACTGGGACCAACATTCCGAAGTATACCATATCCACTTCATCTCCAATCAGAAATACTTCAGACCCTACATTATTTCAGACTGCAAGGATCTCTGCTTCATCACTCAGATACTATGGCTTGGGTCTTGAGAACGGAAACTACACCGTGAAGCTTGAATTTGCAGAACAAGCTATCCTGGATGAGTCTACAAGAAAAAGTCATGGAAGGCGTGTGTTTGATATATACATCCAGGACGTACTTGTTGCTAAAGATTTTGATATACGAAACGAGACAAATAGGACATCTTTGGTAGCTATTGAAATGGTATACAACGCTCAGGTGGTTTCAGAGAATTACCTTGAAATCCATTTCTTCTGGGCTGGAAAGGGGACCTGCTGCATCCCAGAGGAGGGTACTTATGGACCTCTTATTTCAGCCATCAGTGCTACACCTGAATTCAAACCTACCGTCAGCAACAAACTGCCAAGTACTAAGAAGAATAGGACTGGGATGATTGTGGGAATTGTTGTTAGTGGGGGGGTTTTAATTCTGTTGGTGCTGCTCTTCTATATTGTTCAAATAAGAAAAAGGCACAACTCCGATGATGACTATGATGAAGATCTGTTGGGGATAGATCTTGGACCTCTCACTTTCAGTTTTTCTGAACTGAAGGCAGCTACGAATGACTTTAGTCCAGATAATAAGCTTGGAGAGGGAGGATTTGGGCCTGTCTTCAAAGGTACTCTAAATGATGGAAGAGTGATTGCCGTAAAACAATTGTCTGCATCATCCCATCAAGGAAAGAACCAGTTTGTGACTGAGATTGCCACTATATCTGCTGTGCGACACAATAACCTAGTAAATCTGTCTGGATTCTGCACTGAGGGAGCTAAACGGCTCCTTGTCTATGAGTATCTGGAGAACAAGAGTGTTGATCAAGCGTTATTTGGAAAAAGAACCTTGCATCTTGATTGGCGAACACGTTTTGATATATGCTTGGGCGTTGCTAGAGGTTTAACATATCTTCATGAAGAATCAAGGCTTCGAATTGTACACAGAGATGTGAAGGCAAGTAACATTCTTCTTGACTCTAATCTCATTGCCAAAATATCAGATTTTGGTTTGGCCAAGCATTATGATGAGAAAAAGACTCACATGAGTACTCGAGTTGCGGGAACAATCGGGTATCTTGCACCGGAATATGCCATGCGTGGACACTTGACTGAGAAGACTGATGTGTTTGCCTATGGTGTTGTCATTCTAGAAATTGTCAGCGGTAGGCCAAATTCTGATCCAAGTTTGGAAGGAGATATGGTTTATCTTCTTGAACTGGCCTGGAACTTGCATGAAAACAAACGTGAAGTTGATCTTGTAGACTCTAGATTATCTGAATTCAATGAGGAAGAAGCAAGACGAATTATTAACATAGGACTTGTGTGCACTCAATCATCACCAAATCTACGACCCTCTATGTCTCGCGTGATCGGAATGCTTACAGGAGATATTGAAGTGACTCCTGCTACTTCGAAGCCTGGTTACTTGACAGACTGGAAATTCGATGATGTAACTACTATCACCAGCCAAACGAGATTCGGTGATGGAAGTACCGGCACCAGTTTAGCAACTGATAATTCAATTAGAGGAACTGACTTCAGCTTTTACAACTCATCGGCAGGTACAAGTGTGATGGGGATTCAGGGCAATTACCTTCAAATACCACCCTTCCAATACTTAGTAGTACAAATGGTGATGGTAGGTAAGAGTTTTGAAGGAACTGAGTAA

Protein Analysis

1093

Amino Acids

120.55

Weight (kDa)

5.19

Isoelectric Point (pI)

37.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 139 - 271 6.3e-11 Leucine-rich repeat region
LRR_4 PF12799 290 - 331 4.1e-06 Leucine Rich repeats (2 copies)
Malectin PF11721 428 - 622 4e-40 Malectin domain
PK_Tyr_Ser-Thr PF07714 708 - 971 1.3e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 708 - 968 1.7e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1350
AatII GACGTC 1 cut(s) 944
Acc36I ACCTGC 2 cut(s) 1898, 3161
AccB1I GGYRCC 1 cut(s) 3107
AccI GTMKAC 5 cut(s) 1285, 1541, 1703, 1824, 2871
AccII CGCG 1 cut(s) 2976
AccIII TCCGGA 1 cut(s) 677
AciI CCGC 5 cut(s) 687, 1368, 1376, 2669, 2772
AclWI GGATC 3 cut(s) 83, 1613, 2783
AcoI YGGCCR 2 cut(s) 1245, 2622
AcsI RAATTY 6 cut(s) 476, 1673, 1952, 2782, 2888, 3050
AcuI CTGAAG 6 cut(s) 117, 1240, 1275, 1560, 2186, 3133
AcyI GRCGYC 1 cut(s) 941
AfeI AGCGCT 1 cut(s) 108
AfiI CCNNNNNNNGG 6 cut(s) 182, 635, 1018, 1312, 1906, 1907
AflIII ACRYGT 1 cut(s) 2477
AjnI CCWGG 5 cut(s) 1026, 1692, 1746, 2831, 3028
AloI GAACNNNNNNTCC 2 cut(s) 1674, 1706
Alw21I GWGCWC 3 cut(s) 348, 1092, 2939
Alw26I GTCTC 3 cut(s) 948, 1781, 2976
Alw44I GTGCAC 1 cut(s) 2935
AlwI GGATC 3 cut(s) 83, 1613, 2783
Ama87I CYCGRG 1 cut(s) 2661
Aor13HI TCCGGA 1 cut(s) 677
Aor51HI AGCGCT 1 cut(s) 108
AoxI GGCC 6 cut(s) 1192, 1245, 2222, 2622, 2777, 2829
ApaLI GTGCAC 1 cut(s) 2935
ApoI RAATTY 6 cut(s) 476, 1673, 1952, 2782, 2888, 3050
ArsI GACNNNNNNTTYG 4 cut(s) 1950, 1982, 3035, 3067
AseI ATTAAT 1 cut(s) 1362
Asp700I GAANNNNTTC 1 cut(s) 1573
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 7 cut(s) 401, 1373, 1524, 1887, 1919, 2138, 2222
AsuHPI GGTGA 9 cut(s) 182, 485, 557, 701, 1016, 2940, 3064, 3104, 3256
AsuII TTCGAA 2 cut(s) 2542, 3023
AvaI CYCGRG 1 cut(s) 2661
AvaII GGWCC 6 cut(s) 401, 1373, 1524, 1887, 1919, 2138
BaeGI GKGCMC 1 cut(s) 2939
BaeI ACNNNNGTAYC 2 cut(s) 3165, 3198
BalI TGGCCA 2 cut(s) 1247, 2624
BanI GGYRCC 1 cut(s) 3107
BanII GRGCYC 2 cut(s) 348, 1425
BarI GAAGNNNNNNTAC 4 cut(s) 822, 854, 3017, 3049
BbsI GAAGAC 2 cut(s) 2221, 2732
Bbv12I GWGCWC 3 cut(s) 348, 1092, 2939
BbvCI CCTCAGC 1 cut(s) 962
BccI CCATC 9 cut(s) 850, 1103, 1458, 1943, 2243, 2298, 3089, 3178, 3241
BceAI ACGGC 2 cut(s) 2249, 2408
BciT130I CCWGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
BclI TGATCA 1 cut(s) 2431
BcoDI GTCTC 3 cut(s) 948, 1781, 2976
BfaI CTAG 5 cut(s) 947, 2354, 2505, 2759, 2877
BfmI CTRYAG 2 cut(s) 30, 40
BfoI RGCGCY 1 cut(s) 110
BfuAI ACCTGC 2 cut(s) 1898, 3161
BglII AGATCT 2 cut(s) 2116, 2131
BlpI GCTNAGC 1 cut(s) 1150
BmcAI AGTACT 2 cut(s) 1987, 2659
Bme1390I CCNGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
Bme18I GGWCC 6 cut(s) 401, 1373, 1524, 1887, 1919, 2138
BmeT110I CYCGRG 1 cut(s) 2661
BmgT120I GGNCC 7 cut(s) 401, 1373, 1524, 1887, 1919, 2138, 2222
BmiI GGNNCC 4 cut(s) 1525, 1888, 2396, 3109
BmrFI CCNGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
BmrI ACTGGG 2 cut(s) 1530, 2013
BmsI GCATC 7 cut(s) 217, 317, 422, 1517, 1905, 2291, 2470
BmuI ACTGGG 2 cut(s) 1530, 2013
BoxI GACNNNNGTC 1 cut(s) 2187
BpiI GAAGAC 2 cut(s) 2221, 2732
BpmI CTGGAG 1 cut(s) 2435
Bpu10I CCTNAGC 2 cut(s) 962, 1833
Bpu1102I GCTNAGC 1 cut(s) 1150
Bpu14I TTCGAA 2 cut(s) 2542, 3023
BpuEI CTTGAG 2 cut(s) 826, 1664
BsaAI YACGTR 1 cut(s) 441
BsaBI GATNNNNATC 1 cut(s) 2115
BsaHI GRCGYC 1 cut(s) 941
BsaJI CCNNGG 1 cut(s) 1426
BsaWI WCCGGW 2 cut(s) 677, 2692
BsaXI ACNNNNNCTCC 4 cut(s) 1543, 1573, 2796, 2826
Bsc4I CCNNNNNNNGG 6 cut(s) 182, 635, 1018, 1312, 1906, 1907
Bse118I RCCGGY 1 cut(s) 3104
Bse3DI GCAATG 3 cut(s) 161, 1483, 2595
Bse8I GATNNNNATC 1 cut(s) 2115
BseAI TCCGGA 1 cut(s) 677
BseBI CCWGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
BseDI CCNNGG 1 cut(s) 1426
BseGI GGATG 7 cut(s) 382, 1095, 1702, 1743, 1896, 2013, 2285
BseJI GATNNNNATC 1 cut(s) 2115
BseLI CCNNNNNNNGG 6 cut(s) 182, 635, 1018, 1312, 1906, 1907
BseMI GCAATG 3 cut(s) 161, 1483, 2595
BseMII CTCAG 9 cut(s) 621, 953, 1164, 1636, 1847, 2307, 2370, 2712, 3264
BseRI GAGGAG 1 cut(s) 1919
BseSI GKGCMC 1 cut(s) 2939
BsgI GTGCAG 2 cut(s) 199, 2359
Bsh1236I CGCG 1 cut(s) 2976
BshFI GGCC 6 cut(s) 1194, 1247, 2224, 2624, 2779, 2831
BshNI GGYRCC 1 cut(s) 3107
BsiHKAI GWGCWC 3 cut(s) 348, 1092, 2939
BsiHKCI CYCGRG 1 cut(s) 2661
BsiSI CCGG 3 cut(s) 678, 2693, 3105
BslFI GGGAC 6 cut(s) 468, 540, 613, 1411, 1537, 1900
BslI CCNNNNNNNGG 6 cut(s) 182, 635, 1018, 1312, 1906, 1907
BsmAI GTCTC 3 cut(s) 948, 1781, 2976
BsmBI CGTCTC 1 cut(s) 948
BsmFI GGGAC 6 cut(s) 468, 540, 613, 1411, 1537, 1900
BsmI GAATGC 1 cut(s) 2991
BsnI GGCC 6 cut(s) 1194, 1247, 2224, 2624, 2779, 2831
BsoBI CYCGRG 1 cut(s) 2661
Bsp119I TTCGAA 2 cut(s) 2542, 3023
Bsp1286I GDGCHC 4 cut(s) 348, 1092, 1425, 2939
Bsp13I TCCGGA 1 cut(s) 677
Bsp1407I TGTACA 2 cut(s) 67, 2548
Bsp143I GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
Bsp1720I GCTNAGC 1 cut(s) 1150
BspACI CCGC 5 cut(s) 687, 1368, 1376, 2669, 2772
BspANI GGCC 6 cut(s) 1194, 1247, 2224, 2624, 2779, 2831
BspCNI CTCAG 9 cut(s) 622, 954, 1163, 1635, 1846, 2308, 2371, 2713, 3265
BspEI TCCGGA 1 cut(s) 677
BspFNI CGCG 1 cut(s) 2976
BspHI TCATGA 2 cut(s) 1356, 2524
BspLI GGNNCC 4 cut(s) 1525, 1888, 2396, 3109
BspMAI CTGCAG 2 cut(s) 34, 44
BspMI ACCTGC 2 cut(s) 1898, 3161
BspPI GGATC 3 cut(s) 83, 1613, 2783
BspQI GCTCTTC 3 cut(s) 336, 1161, 2066
BspT104I TTCGAA 2 cut(s) 2542, 3023
BspT107I GGYRCC 1 cut(s) 3107
BsrDI GCAATG 3 cut(s) 161, 1483, 2595
BsrFI RCCGGY 1 cut(s) 3104
BsrGI TGTACA 2 cut(s) 67, 2548
BssAI RCCGGY 1 cut(s) 3104
BssECI CCNNGG 1 cut(s) 1426
BssMI GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
BssNAI GTATAC 2 cut(s) 1542, 1825
BssNI GRCGYC 1 cut(s) 941
BssT1I CCWWGG 1 cut(s) 1426
Bst1107I GTATAC 2 cut(s) 1542, 1825
Bst2UI CCWGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
Bst4CI ACNGT 8 cut(s) 263, 852, 1268, 1331, 1402, 1418, 1663, 1966
Bst6I CTCTTC 5 cut(s) 336, 919, 1161, 2066, 2248
BstACI GRCGYC 1 cut(s) 941
BstAUI TGTACA 2 cut(s) 67, 2548
BstBAI YACGTR 1 cut(s) 441
BstBI TTCGAA 2 cut(s) 2542, 3023
BstC8I GCNNGC 3 cut(s) 40, 764, 1092
BstEII GGTNACC 1 cut(s) 170
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 7 cut(s) 382, 1095, 1702, 1743, 1896, 2013, 2285
BstFNI CGCG 1 cut(s) 2976
BstH2I RGCGCY 1 cut(s) 110
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 8 cut(s) 91, 1608, 2119, 2134, 2434, 2791, 2866, 2982
BstMAI GTCTC 3 cut(s) 948, 1781, 2976
BstMBI GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
BstMWI GCNNNNNNNGC 5 cut(s) 35, 113, 1323, 1685, 1940
BstNI CCWGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
BstPAI GACNNNNGTC 1 cut(s) 2187
BstPI GGTNACC 1 cut(s) 170
BstSCI CCNGG 5 cut(s) 1026, 1692, 1746, 2831, 3028
BstSFI CTRYAG 2 cut(s) 30, 40
BstSLI GKGCMC 1 cut(s) 2939
BstUI CGCG 1 cut(s) 2976
BstV2I GAAGAC 2 cut(s) 2221, 2732
BstX2I RGATCY 4 cut(s) 88, 1605, 2116, 2131
BstYI RGATCY 4 cut(s) 88, 1605, 2116, 2131
BstZ17I GTATAC 2 cut(s) 1542, 1825
BsuRI GGCC 6 cut(s) 1194, 1247, 2224, 2624, 2779, 2831
BtsCI GGATG 7 cut(s) 382, 1095, 1702, 1743, 1896, 2013, 2285
BtsI GCAGTG 2 cut(s) 187, 1285
BtsIMutI CAGTG 7 cut(s) 187, 756, 848, 1285, 1423, 1945, 2376
BveI ACCTGC 2 cut(s) 1898, 3161
Cac8I GCNNGC 3 cut(s) 40, 764, 1092
CciI TCATGA 2 cut(s) 1356, 2524
CfoI GCGC 1 cut(s) 109
Cfr10I RCCGGY 1 cut(s) 3104
Cfr13I GGNCC 7 cut(s) 401, 1373, 1524, 1887, 1919, 2138, 2222
CseI GACGC 1 cut(s) 424
CviAII CATG 6 cut(s) 1357, 1718, 2525, 2653, 2704, 2843
DpnI GATC 8 cut(s) 90, 1607, 2118, 2133, 2433, 2790, 2865, 2981
DpnII GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
EaeI YGGCCR 2 cut(s) 1245, 2622
Eam1104I CTCTTC 5 cut(s) 336, 919, 1161, 2066, 2248
EarI CTCTTC 5 cut(s) 336, 919, 1161, 2066, 2248
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 1426
Eco24I GRGCYC 2 cut(s) 348, 1425
Eco32I GATATC 1 cut(s) 1389
Eco47I GGWCC 6 cut(s) 401, 1373, 1524, 1887, 1919, 2138
Eco47III AGCGCT 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 6 cut(s) 117, 1240, 1275, 1560, 2186, 3133
Eco88I CYCGRG 1 cut(s) 2661
Eco91I GGTNACC 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 346
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoO109I RGGNCCY 2 cut(s) 401, 1887
EcoO65I GGTNACC 1 cut(s) 170
EcoRI GAATTC 2 cut(s) 1952, 2888
EcoRII CCWGG 5 cut(s) 1026, 1692, 1746, 2831, 3028
EcoRV GATATC 1 cut(s) 1389
EcoT14I CCWWGG 1 cut(s) 1426
EcoT38I GRGCYC 2 cut(s) 348, 1425
ErhI CCWWGG 1 cut(s) 1426
Esp3I CGTCTC 1 cut(s) 948
FaeI CATG 6 cut(s) 1360, 1721, 2528, 2656, 2707, 2846
FalI AAGNNNNNCTT 2 cut(s) 2850, 2882
FaqI GGGAC 6 cut(s) 468, 540, 613, 1411, 1537, 1900
FatI CATG 6 cut(s) 1356, 1717, 2524, 2652, 2703, 2842
FauI CCCGC 1 cut(s) 2662
FbaI TGATCA 1 cut(s) 2431
FblI GTMKAC 5 cut(s) 1285, 1541, 1703, 1824, 2871
FokI GGATG 7 cut(s) 389, 1082, 1709, 1730, 1883, 2020, 2272
FriOI GRGCYC 2 cut(s) 348, 1425
FspBI CTAG 5 cut(s) 947, 2354, 2505, 2759, 2877
GlaI GCGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 2435
HaeII RGCGCY 1 cut(s) 110
HaeIII GGCC 6 cut(s) 1194, 1247, 2224, 2624, 2779, 2831
HapII CCGG 3 cut(s) 678, 2693, 3105
HgaI GACGC 1 cut(s) 424
HhaI GCGC 1 cut(s) 109
Hin1I GRCGYC 1 cut(s) 941
Hin1II CATG 6 cut(s) 1360, 1721, 2528, 2656, 2707, 2846
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HindIII AAGCTT 3 cut(s) 794, 1667, 2201
HpaII CCGG 3 cut(s) 678, 2693, 3105
HphI GGTGA 9 cut(s) 182, 485, 557, 701, 1016, 2940, 3064, 3104, 3256
Hpy166II GTNNAC 9 cut(s) 1024, 1286, 1542, 1704, 1825, 2551, 2711, 2872, 2937
Hpy8I GTNNAC 9 cut(s) 1024, 1286, 1542, 1704, 1825, 2551, 2711, 2872, 2937
HpyCH4III ACNGT 8 cut(s) 263, 852, 1268, 1331, 1402, 1418, 1663, 1966
HpyCH4IV ACGT 5 cut(s) 440, 941, 1752, 2479, 2854
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 113, 1323, 1685, 1940
HpySE526I ACGT 5 cut(s) 440, 941, 1752, 2479, 2854
Hsp92I GRCGYC 1 cut(s) 941
Hsp92II CATG 6 cut(s) 1360, 1721, 2528, 2656, 2707, 2846
HspAI GCGC 1 cut(s) 107
Kpn2I TCCGGA 1 cut(s) 677
Ksp22I TGATCA 1 cut(s) 2431
Kzo9I GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
LguI GCTCTTC 3 cut(s) 336, 1161, 2066
LmnI GCTCC 4 cut(s) 334, 727, 2384, 2400
LweI GCATC 7 cut(s) 217, 317, 422, 1517, 1905, 2291, 2470
MaeI CTAG 5 cut(s) 947, 2354, 2505, 2759, 2877
MaeII ACGT 5 cut(s) 440, 941, 1752, 2479, 2854
MaeIII GTNAC 8 cut(s) 170, 436, 1447, 2311, 2570, 3007, 3032, 3061
MalI GATC 8 cut(s) 90, 1607, 2118, 2133, 2433, 2790, 2865, 2981
MboI GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
MfeI CAATTG 2 cut(s) 417, 2273
MflI RGATCY 4 cut(s) 88, 1605, 2116, 2131
MhlI GDGCHC 4 cut(s) 348, 1092, 1425, 2939
MlsI TGGCCA 2 cut(s) 1247, 2624
MluNI TGGCCA 2 cut(s) 1247, 2624
MlyI GAGTC 6 cut(s) 856, 1709, 2579, 2641, 2867, 3004
MmeI TCCRAC 2 cut(s) 1020, 1033
Mox20I TGGCCA 2 cut(s) 1247, 2624
MroI TCCGGA 1 cut(s) 677
MroXI GAANNNNTTC 1 cut(s) 1573
MscI TGGCCA 2 cut(s) 1247, 2624
MslI CAYNNNNRTG 2 cut(s) 2556, 2634
Msp20I TGGCCA 2 cut(s) 1247, 2624
MspA1I CMGCKG 1 cut(s) 2772
MspI CCGG 3 cut(s) 678, 2693, 3105
MspR9I CCNGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
MunI CAATTG 2 cut(s) 417, 2273
Mva1269I GAATGC 1 cut(s) 2991
MvaI CCWGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
MvnI CGCG 1 cut(s) 2976
MwoI GCNNNNNNNGC 5 cut(s) 35, 113, 1323, 1685, 1940
NdeII GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
NlaIII CATG 6 cut(s) 1360, 1721, 2528, 2656, 2707, 2846
NlaIV GGNNCC 4 cut(s) 1525, 1888, 2396, 3109
NmuCI GTSAC 5 cut(s) 170, 436, 1447, 2311, 3007
NspV TTCGAA 2 cut(s) 2542, 3023
PaeR7I CTCGAG 1 cut(s) 2661
PagI TCATGA 2 cut(s) 1356, 2524
PciSI GCTCTTC 3 cut(s) 336, 1161, 2066
PctI GAATGC 1 cut(s) 2991
PdmI GAANNNNTTC 1 cut(s) 1573
PfeI GAWTC 7 cut(s) 272, 694, 983, 2370, 2531, 3087, 3190
PfoI TCCNGGA 2 cut(s) 1692, 1746
PleI GAGTC 6 cut(s) 856, 1708, 2579, 2641, 2867, 3004
PpsI GAGTC 6 cut(s) 856, 1708, 2579, 2641, 2867, 3004
Ppu21I YACGTR 1 cut(s) 441
PpuMI RGGWCCY 2 cut(s) 401, 1887
PshAI GACNNNNGTC 1 cut(s) 2187
PshBI ATTAAT 1 cut(s) 1362
PsiI TTATAA 1 cut(s) 1350
Psp124BI GAGCTC 1 cut(s) 348
Psp5II RGGWCCY 2 cut(s) 401, 1887
Psp6I CCWGG 5 cut(s) 1026, 1692, 1746, 2831, 3028
PspEI GGTNACC 1 cut(s) 170
PspGI CCWGG 5 cut(s) 1026, 1692, 1746, 2831, 3028
PspN4I GGNNCC 4 cut(s) 1525, 1888, 2396, 3109
PspPI GGNCC 7 cut(s) 401, 1373, 1524, 1887, 1919, 2138, 2222
PspPPI RGGWCCY 2 cut(s) 401, 1887
PspXI VCTCGAGB 1 cut(s) 2661
PsrI GAACNNNNNNTAC 4 cut(s) 1212, 1244, 1640, 1672
PstI CTGCAG 2 cut(s) 34, 44
PsuI RGATCY 4 cut(s) 88, 1605, 2116, 2131
RseI CAYNNNNRTG 2 cut(s) 2556, 2634
SacI GAGCTC 1 cut(s) 348
SapI GCTCTTC 3 cut(s) 336, 1161, 2066
Sau3AI GATC 8 cut(s) 88, 1605, 2116, 2131, 2431, 2788, 2863, 2979
Sau96I GGNCC 7 cut(s) 401, 1373, 1524, 1887, 1919, 2138, 2222
ScaI AGTACT 2 cut(s) 1987, 2659
SchI GAGTC 6 cut(s) 856, 1709, 2579, 2641, 2867, 3004
ScrFI CCNGG 5 cut(s) 1028, 1694, 1748, 2833, 3030
SduI GDGCHC 4 cut(s) 348, 1092, 1425, 2939
SfaNI GCATC 7 cut(s) 217, 317, 422, 1517, 1905, 2291, 2470
SfcI CTRYAG 2 cut(s) 30, 40
Sfr274I CTCGAG 1 cut(s) 2661
SfuI TTCGAA 2 cut(s) 2542, 3023
SinI GGWCC 6 cut(s) 401, 1373, 1524, 1887, 1919, 2138
SlaI CTCGAG 1 cut(s) 2661
SmiMI CAYNNNNRTG 2 cut(s) 2556, 2634
SmlI CTYRAG 3 cut(s) 805, 1643, 2661
SmoI CTYRAG 3 cut(s) 805, 1643, 2661
SsiI CCGC 5 cut(s) 687, 1368, 1376, 2669, 2772
SspI AATATT 3 cut(s) 136, 661, 976
SspMI CTAG 5 cut(s) 947, 2354, 2505, 2759, 2877
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 5 cut(s) 1026, 1692, 1746, 2831, 3028
StyI CCWWGG 1 cut(s) 1426
TaaI ACNGT 8 cut(s) 263, 852, 1268, 1331, 1402, 1418, 1663, 1966
TaiI ACGT 5 cut(s) 443, 944, 1755, 2482, 2857
TaqI TCGA 5 cut(s) 606, 2542, 2662, 3023, 3054
TaqII GACCGA 1 cut(s) 1472
TatI WGTACW 5 cut(s) 67, 1985, 2548, 2657, 3235
TfiI GAWTC 7 cut(s) 272, 694, 983, 2370, 2531, 3087, 3190
TscAI CASTG 7 cut(s) 187, 763, 855, 1285, 1423, 1945, 2383
TseFI GTSAC 5 cut(s) 170, 436, 1447, 2311, 3007
Tsp45I GTSAC 5 cut(s) 170, 436, 1447, 2311, 3007
TspGWI ACGGA 2 cut(s) 234, 1665
TspRI CASTG 7 cut(s) 187, 763, 855, 1285, 1423, 1945, 2383
VneI GTGCAC 1 cut(s) 2935
VpaK11BI GGWCC 6 cut(s) 401, 1373, 1524, 1887, 1919, 2138
VspI ATTAAT 1 cut(s) 1362
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 6 cut(s) 476, 1673, 1952, 2782, 2888, 3050
XbaI TCTAGA 3 cut(s) 946, 2758, 2876
XhoI CTCGAG 1 cut(s) 2661
XmiI GTMKAC 5 cut(s) 1285, 1541, 1703, 1824, 2871
XmnI GAANNNNTTC 1 cut(s) 1573
XspI CTAG 5 cut(s) 947, 2354, 2505, 2759, 2877
ZraI GACGTC 1 cut(s) 942
ZrmI AGTACT 2 cut(s) 1987, 2659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.