Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 494 |
| AccI | GTMKAC | 1 cut(s) | 273 |
| AccII | CGCG | 1 cut(s) | 378 |
| AciI | CCGC | 2 cut(s) | 42, 174 |
| AclWI | GGATC | 1 cut(s) | 185 |
| AcsI | RAATTY | 2 cut(s) | 184, 290 |
| AcuI | CTGAAG | 1 cut(s) | 520 |
| AfaI | GTAC | 3 cut(s) | 61, 490, 562 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 35, 36, 553 |
| AgsI | TTSAA | 5 cut(s) | 227, 295, 407, 426, 597 |
| AluBI | AGCT | 1 cut(s) | 540 |
| AluI | AGCT | 1 cut(s) | 540 |
| Alw21I | GWGCWC | 1 cut(s) | 341 |
| Alw26I | GTCTC | 1 cut(s) | 378 |
| Alw44I | GTGCAC | 1 cut(s) | 337 |
| AlwI | GGATC | 1 cut(s) | 185 |
| Ama87I | CYCGRG | 1 cut(s) | 63 |
| AoxI | GGCC | 1 cut(s) | 179 |
| ApaLI | GTGCAC | 1 cut(s) | 337 |
| ApoI | RAATTY | 2 cut(s) | 184, 290 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 437, 469 |
| AspS9I | GGNCC | 1 cut(s) | 39 |
| AsuC2I | CCSGG | 1 cut(s) | 432 |
| AsuHPI | GGTGA | 2 cut(s) | 342, 644 |
| AvaI | CYCGRG | 1 cut(s) | 63 |
| AvaII | GGWCC | 1 cut(s) | 39 |
| BaeGI | GKGCMC | 2 cut(s) | 52, 341 |
| BanI | GGYRCC | 1 cut(s) | 494 |
| BanII | GRGCYC | 1 cut(s) | 20 |
| Bbv12I | GWGCWC | 1 cut(s) | 341 |
| BccI | CCATC | 2 cut(s) | 565, 629 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 122, 156 |
| BcnI | CCSGG | 1 cut(s) | 432 |
| BcoDI | GTCTC | 1 cut(s) | 378 |
| BfaI | CTAG | 5 cut(s) | 161, 269, 279, 503, 522 |
| BmcAI | AGTACT | 2 cut(s) | 61, 490 |
| Bme1390I | CCNGG | 1 cut(s) | 432 |
| Bme18I | GGWCC | 1 cut(s) | 39 |
| BmeT110I | CYCGRG | 1 cut(s) | 63 |
| BmgT120I | GGNCC | 1 cut(s) | 39 |
| BmiI | GGNNCC | 2 cut(s) | 40, 496 |
| BmrFI | CCNGG | 1 cut(s) | 432 |
| BmsI | GCATC | 1 cut(s) | 568 |
| BpmI | CTGGAG | 1 cut(s) | 469 |
| BpuMI | CCSGG | 1 cut(s) | 432 |
| BsaWI | WCCGGW | 1 cut(s) | 94 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 35, 36, 553 |
| Bse1I | ACTGG | 4 cut(s) | 45, 452, 496, 498 |
| BseGI | GGATG | 1 cut(s) | 583 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 35, 36, 553 |
| BseNI | ACTGG | 4 cut(s) | 45, 452, 496, 498 |
| BseSI | GKGCMC | 2 cut(s) | 52, 341 |
| Bsh1236I | CGCG | 1 cut(s) | 378 |
| BshFI | GGCC | 1 cut(s) | 181 |
| BshNI | GGYRCC | 1 cut(s) | 494 |
| BsiHKAI | GWGCWC | 1 cut(s) | 341 |
| BsiHKCI | CYCGRG | 1 cut(s) | 63 |
| BsiSI | CCGG | 2 cut(s) | 95, 432 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 35, 36, 553 |
| BsmAI | GTCTC | 1 cut(s) | 378 |
| BsmI | GAATGC | 1 cut(s) | 393 |
| BsnI | GGCC | 1 cut(s) | 181 |
| BsoBI | CYCGRG | 1 cut(s) | 63 |
| Bsp1286I | GDGCHC | 3 cut(s) | 20, 52, 341 |
| Bsp143I | GATC | 2 cut(s) | 190, 265 |
| BspACI | CCGC | 2 cut(s) | 42, 174 |
| BspANI | GGCC | 1 cut(s) | 181 |
| BspFNI | CGCG | 1 cut(s) | 378 |
| BspLI | GGNNCC | 2 cut(s) | 40, 496 |
| BspPI | GGATC | 1 cut(s) | 185 |
| BspT107I | GGYRCC | 1 cut(s) | 494 |
| BsrI | ACTGG | 4 cut(s) | 45, 452, 496, 498 |
| BssMI | GATC | 2 cut(s) | 190, 265 |
| Bst6I | CTCTTC | 1 cut(s) | 612 |
| BstC8I | GCNNGC | 1 cut(s) | 393 |
| BstF5I | GGATG | 1 cut(s) | 583 |
| BstFNI | CGCG | 1 cut(s) | 378 |
| BstKTI | GATC | 2 cut(s) | 193, 268 |
| BstMAI | GTCTC | 1 cut(s) | 378 |
| BstMBI | GATC | 2 cut(s) | 190, 265 |
| BstSCI | CCNGG | 1 cut(s) | 430 |
| BstSLI | GKGCMC | 2 cut(s) | 52, 341 |
| BstUI | CGCG | 1 cut(s) | 378 |
| BsuRI | GGCC | 1 cut(s) | 181 |
| BtsCI | GGATG | 1 cut(s) | 583 |
| BtsIMutI | CAGTG | 1 cut(s) | 52 |
| Cac8I | GCNNGC | 1 cut(s) | 393 |
| Cfr13I | GGNCC | 1 cut(s) | 39 |
| Csp6I | GTAC | 3 cut(s) | 60, 489, 561 |
| CviAII | CATG | 2 cut(s) | 55, 245 |
| CviJI | RGCY | 6 cut(s) | 18, 181, 233, 430, 471, 540 |
| CviKI_1 | RGCY | 6 cut(s) | 18, 181, 233, 430, 471, 540 |
| CviQI | GTAC | 3 cut(s) | 60, 489, 561 |
| DpnI | GATC | 2 cut(s) | 192, 267 |
| DpnII | GATC | 2 cut(s) | 190, 265 |
| Eam1104I | CTCTTC | 1 cut(s) | 612 |
| EarI | CTCTTC | 1 cut(s) | 612 |
| EciI | GGCGGA | 1 cut(s) | 31 |
| Eco24I | GRGCYC | 1 cut(s) | 20 |
| Eco47I | GGWCC | 1 cut(s) | 39 |
| Eco57I | CTGAAG | 1 cut(s) | 520 |
| Eco88I | CYCGRG | 1 cut(s) | 63 |
| EcoRI | GAATTC | 1 cut(s) | 290 |
| EcoT38I | GRGCYC | 1 cut(s) | 20 |
| FaeI | CATG | 2 cut(s) | 58, 248 |
| FatI | CATG | 2 cut(s) | 54, 244 |
| FblI | GTMKAC | 1 cut(s) | 273 |
| FokI | GGATG | 1 cut(s) | 590 |
| FriOI | GRGCYC | 1 cut(s) | 20 |
| FspBI | CTAG | 5 cut(s) | 161, 269, 279, 503, 522 |
| GsuI | CTGGAG | 1 cut(s) | 469 |
| HaeIII | GGCC | 1 cut(s) | 181 |
| HapII | CCGG | 2 cut(s) | 95, 432 |
| Hin1II | CATG | 2 cut(s) | 58, 248 |
| HincII | GTYRAC | 1 cut(s) | 519 |
| HindII | GTYRAC | 1 cut(s) | 519 |
| HinfI | GANTC | 2 cut(s) | 275, 412 |
| HpaII | CCGG | 2 cut(s) | 95, 432 |
| HphI | GGTGA | 2 cut(s) | 342, 644 |
| Hpy166II | GTNNAC | 4 cut(s) | 113, 274, 339, 519 |
| Hpy188I | TCNGA | 2 cut(s) | 190, 289 |
| Hpy188III | TCNNGA | 3 cut(s) | 161, 224, 279 |
| Hpy8I | GTNNAC | 4 cut(s) | 113, 274, 339, 519 |
| HpyAV | CCTTC | 1 cut(s) | 603 |
| HpyCH4IV | ACGT | 1 cut(s) | 256 |
| HpyCH4V | TGCA | 6 cut(s) | 71, 92, 244, 339, 395, 581 |
| HpySE526I | ACGT | 1 cut(s) | 256 |
| Hsp92II | CATG | 2 cut(s) | 58, 248 |
| Kzo9I | GATC | 2 cut(s) | 190, 265 |
| LweI | GCATC | 1 cut(s) | 568 |
| MaeI | CTAG | 5 cut(s) | 161, 269, 279, 503, 522 |
| MaeII | ACGT | 1 cut(s) | 256 |
| MaeIII | GTNAC | 3 cut(s) | 409, 434, 463 |
| MalI | GATC | 2 cut(s) | 192, 267 |
| MboI | GATC | 2 cut(s) | 190, 265 |
| MboII | GAAGA | 3 cut(s) | 212, 314, 599 |
| MhlI | GDGCHC | 3 cut(s) | 20, 52, 341 |
| MluCI | AATT | 6 cut(s) | 165, 184, 227, 290, 315, 587 |
| MlyI | GAGTC | 2 cut(s) | 269, 406 |
| MnlI | CCTC | 3 cut(s) | 41, 292, 518 |
| MspA1I | CMGCKG | 1 cut(s) | 174 |
| MspI | CCGG | 2 cut(s) | 95, 432 |
| MspR9I | CCNGG | 1 cut(s) | 432 |
| Mva1269I | GAATGC | 1 cut(s) | 393 |
| MvnI | CGCG | 1 cut(s) | 378 |
| NciI | CCSGG | 1 cut(s) | 432 |
| NdeII | GATC | 2 cut(s) | 190, 265 |
| NlaIII | CATG | 2 cut(s) | 58, 248 |
| NlaIV | GGNNCC | 2 cut(s) | 40, 496 |
| NmuCI | GTSAC | 1 cut(s) | 409 |
| PctI | GAATGC | 1 cut(s) | 393 |
| PleI | GAGTC | 2 cut(s) | 269, 406 |
| PpsI | GAGTC | 2 cut(s) | 269, 406 |
| PspN4I | GGNNCC | 2 cut(s) | 40, 496 |
| PspPI | GGNCC | 1 cut(s) | 39 |
| RsaI | GTAC | 3 cut(s) | 61, 490, 562 |
| RsaNI | GTAC | 3 cut(s) | 60, 489, 561 |
| Sau3AI | GATC | 2 cut(s) | 190, 265 |
| Sau96I | GGNCC | 1 cut(s) | 39 |
| ScaI | AGTACT | 2 cut(s) | 61, 490 |
| SchI | GAGTC | 2 cut(s) | 269, 406 |
| ScrFI | CCNGG | 1 cut(s) | 432 |
| SduI | GDGCHC | 3 cut(s) | 20, 52, 341 |
| SetI | ASST | 5 cut(s) | 259, 370, 542, 595, 644 |
| SfaNI | GCATC | 1 cut(s) | 568 |
| SinI | GGWCC | 1 cut(s) | 39 |
| Sse9I | AATT | 6 cut(s) | 165, 184, 227, 290, 315, 587 |
| SsiI | CCGC | 2 cut(s) | 42, 174 |
| SspMI | CTAG | 5 cut(s) | 161, 269, 279, 503, 522 |
| StyD4I | CCNGG | 1 cut(s) | 430 |
| TaiI | ACGT | 1 cut(s) | 259 |
| TasI | AATT | 6 cut(s) | 165, 184, 227, 290, 315, 587 |
| TatI | WGTACW | 3 cut(s) | 59, 488, 560 |
| TscAI | CASTG | 1 cut(s) | 52 |
| TseFI | GTSAC | 1 cut(s) | 409 |
| Tsp45I | GTSAC | 1 cut(s) | 409 |
| TspDTI | ATGAA | 1 cut(s) | 261 |
| TspRI | CASTG | 1 cut(s) | 52 |
| VneI | GTGCAC | 1 cut(s) | 337 |
| VpaK11BI | GGWCC | 1 cut(s) | 39 |
| XapI | RAATTY | 2 cut(s) | 184, 290 |
| XbaI | TCTAGA | 2 cut(s) | 160, 278 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 479 |
| XmiI | GTMKAC | 1 cut(s) | 273 |
| XspI | CTAG | 5 cut(s) | 161, 269, 279, 503, 522 |
| ZrmI | AGTACT | 2 cut(s) | 61, 490 |