MD05G1261100.v1.1

Di-glucose binding within endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000088
Physical Location & Seq
Reverse (-)
39636141 .. 39644126
7986 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1261100.v1.1.491

Sequence Viewer

Length: 3150 bp
ATGTACAATATGAAGATGAAGTTGATGACGAGGATACTATTACTCTGCTTCCTCAGCTGCTTCTGGTTTCAGCTGTCCTTTGCTCAAAATGCTACCACTGATCCATCTGAAGTGAGGGCGTTGAACTCAATATTTGAACAATGGGACACACAACCGGTGCCGGGGCTGTGGAATATCAGTGGAGAACCCTGCAGTGGATCTGCCATCAACGGCAACGACTTTGATAGCCCCGATAACAACCCAGCCATCGTTTGCGACTGTTCTTACAACAACAATGCTACATGCCACATCACCAAACTGAGAGTGCAAGCTCTGTACAGACGAGGGGTGTTTCCAGAAGAACTTTTGGCTCTAAGATATCTCGCAGTTTTGAAAATCGATATGAATTATTTCAAAGGTCACCTACCGGCATTCATTGGCAAAATGTCTGCATTGATTGTATTGTCAATTGGCCACAATTCATTCTCTGGGCCCATCCCCAAGGAGATTGGAAACCTTAAGGAGCTAAATATGCTGGCCATCGGAACAAATAATTTTTCCGGAACACTCCCTCCAGAACTTGGTAATTTAGTCAAGCTACAGCAAATTTACATGGACAGCTGTGGACTCAGTGGTGAAATTCCTTCAACATTTGCCAAGCTCACCAGTACGCAAGTCTTTTGGGTATCGGACAGTCCTTTCTCAGGAAAGATACCTGATTTCATAGGGAATTGGACACAACTCACTTCTTTGCGATTTCAAGGGAACTCTTTCGAAGGCCCAATACCAACCAGCTTTTCTCAACTGACCTCATTGAAGTCTCTGCAAATCAGTGATATATACCATGGGAGGAGCTCCTCTCTTGATTTCATAAAAAAAATGAAGAACTTGACTGATTTAAAACTACGAAACGCATTAATCACTGGTACCATCCCTTCTGATATTGGAGAATATCAAAGTCTTAAGATACTGGATCTGAGTTTCAACAATTTGACAGGCCAACTCCCAAGTTCTTTGTTCAGCATGAGTTCTCTTACATCCTTGTTTCTTGGAAACAATAGTCTGTCCGGACCTCTTCCAAGCCAAAAGAGCAATCGACTTCAGACTATAGACTTGTCTTACAATTTTTTATCAGGAAGCTTTCCCCAGTGGGTGACCACAATATCGCAACTGAACTTAGTGGTCAACAACTTCACATTTGACAAGTCAAACATAACTACTCTTCCTGGATTGAATTGCCTCCAGAGAAATTTTCCATGCAATCGAGATACCCCACGATATGCAAACTTCTCAATCAACTGTGGTGGACCGGAAATGAAGGGACGTGATGGCATATTGTATGATGCTGAAGACTCAGCTCTTGGTCCAGCAACATTCAATGTAACCAGTATAGAGAAATGGGCTGTCAGCAATGTCGGTATGTTTTCTGAAGGAGTGAACCGATCGTTTCTGGAAAATACCCCCGAACAAGTCACCGGAACAGATGTGACCCCGGAGCTCTTCCAGACTTCAAGATTGTCCCCAGGATCACTGAGATACTATGGCCTGGGTCTTGAGAATGGACCTTACACTGTAACATTGCACTTTGCAGAGACGGATTTTGAGAAGCGCATTCAGCAAACTTGGGAAAGTCTAGGACGGCGTGTATTTGATATCTATATTCAGGGTACCCGCAGGGAAGAGGACTTTGACATATCCAAGGAGGCACATGGGGTTAACGTAGGAATTAAGAAAATATTCAATGTTAGCGTGTCAGAGAATTATCTTGAAATTCATCTATTCTGGGCTGGTAAAGGGACTTGTTGCATACCTTTACCAGGTGATTACGGCCCACTAATAGCAGCCGTCCATGCTGCTTCAGATTTTACACCAACAGTTTCTGGGCTTCCACCAACTACTCCAGGAAAGAAGAGCAGGACTGGGTTGATAGTTGGTATTGCAGTTCCTGTTGGAGTTGTGAGCTTGTTACTAATATTGGCGATTCTATATATGAGGAGGAAAACATCAGAAAAAGAGGACAACGAAGATATTCTTGGATTAGGCCCTCGACCAAATACTTTCAGTTATGCTGAGTTGAGAGTTGCAACTGAAGATTTTAATCCTTCAAATAAGTTAGGAGAGGGAGGATATGGCCCTGTTTATAAGGGTACACTTTCTGATGGGAGAGTAGTGGCTGTGAAGCAACTTTCAGTAGCATCTCACCAAGGGCAGAGTCAATTTGTATCTGAAATTGCTACCATATCTGCTGTGCAACATAGGAATCTAGTGAAATTGTATGGATGCTGCATCGAAGGCAGCCAGCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGAACAAGTAACTTGCACCTTGACTGGCCTACTCGATTCAATATATTGTTAGGAACAGCAAGAGGACTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCGAGTAATATTTTGCTCGATGCAGAACTCTCCCCAAAAATATCAGATTTTGGACTGGCAAAGCTTTATGATGACAAAAAAACCCACATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCAGAGTATGCATTGTTTGGACATTTGACAGAGAAGGCTGATGTGTTTGGTTTTGGAGTCGTTGTTTTGGAGATCCTCAGTGGGAGACCAAACTCTTACAATAACTTGGATCCAGAAAAGATTTATCTTCTTGAATGGGTATGGACTCTACATGAAAATGACCAAACTCTGGGGTTGGTGGATCCGAGATTGACAGAGTTTGATGAAACCGAAGCAACTAGATTGATAAGAGCAGCTCTCCTGTGCACGCAGGGATCACCGATGGCAAGGCCATCTATGTCACGCGTGGTTGCAATGCTCTCTGGAGACATTGACACAGGCACTGTCATGTCGAAACCAAGCTATTTGACAGATTATAATTTTAAAGATGTAACAACATTTTCAACAGGTAGATTTTTGGTGGAGGATGATACCTCATCAACTGCATCCAAGGATAGTAATGTTCGCCTCAACTATCAGCCGGGAGGCAGCAATGCAAGTGGTGCTGACACTCCCTGGATTGACCCTGCGCCTTCTCCTGTAAACGTCACTCAATCACTGCTCGCTGGCATTAGAAGAGAAGGAAGGTGA

Protein Analysis

1050

Amino Acids

115.65

Weight (kDa)

5.84

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 88 - 197 4.9e-08 Leucine-rich repeat region
LRR_14 PF23598 186 - 294 1.3e-07 Leucine-rich repeat region
LRR_14 PF23598 258 - 354 9e-08 Leucine-rich repeat region
Malectin PF11721 423 - 609 9e-38 Malectin domain
Pkinase PF00069 694 - 956 1.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 695 - 957 3.4e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2121, 2937
Acc65I GGTACC 2 cut(s) 905, 1646
AccB1I GGYRCC 4 cut(s) 157, 905, 1646, 2578
AccB7I CCANNNNNTGG 2 cut(s) 560, 2749
AccII CGCG 1 cut(s) 2865
AccIII TCCGGA 2 cut(s) 539, 1046
AciI CCGC 1 cut(s) 1651
AcoI YGGCCR 2 cut(s) 451, 516
AcsI RAATTY 4 cut(s) 585, 618, 1228, 1749
AcuI CTGAAG 6 cut(s) 129, 1064, 1347, 1428, 1821, 2088
AfaI GTAC 7 cut(s) 5, 317, 649, 907, 1648, 2128, 2439
AfiI CCNNNNNNNGG 8 cut(s) 161, 194, 560, 683, 1796, 2323, 2416, 2749
AflII CTTAAG 2 cut(s) 497, 941
AflIII ACRYGT 1 cut(s) 2863
AgeI ACCGGT 1 cut(s) 154
AjiI CACGTC 1 cut(s) 1304
AjnI CCWGG 6 cut(s) 1204, 1501, 1524, 1795, 1879, 3074
AjuI GAANNNNNNNTTGG 4 cut(s) 760, 792, 1995, 2027
AloI GAACNNNNNNTCC 2 cut(s) 535, 567
Alw21I GWGCWC 3 cut(s) 836, 1479, 2828
Alw26I GTCTC 4 cut(s) 804, 1565, 2659, 2880
Alw44I GTGCAC 1 cut(s) 2824
AlwNI CAGNNNCTG 3 cut(s) 1859, 1925, 2903
Ama87I CYCGRG 1 cut(s) 2550
Aor13HI TCCGGA 2 cut(s) 539, 1046
ApaI GGGCCC 1 cut(s) 474
ApaLI GTGCAC 1 cut(s) 2824
ApeKI GCWGC 7 cut(s) 57, 1820, 1832, 2262, 2274, 2813, 3048
ApoI RAATTY 4 cut(s) 585, 618, 1228, 1749
AseI ATTAAT 1 cut(s) 896
AsiGI ACCGGT 1 cut(s) 154
Asp700I GAANNNNTTC 4 cut(s) 389, 749, 1715, 2007
Asp718I GGTACC 2 cut(s) 905, 1646
AspLEI GCGC 3 cut(s) 1590, 2283, 3091
AsuC2I CCSGG 5 cut(s) 162, 1472, 2551, 2552, 3042
AsuHPI GGTGA 9 cut(s) 283, 392, 626, 634, 1144, 1444, 1811, 2171, 2829
AsuII TTCGAA 1 cut(s) 753
AvaI CYCGRG 1 cut(s) 2550
AvaII GGWCC 4 cut(s) 1049, 1286, 1343, 1541
BaeGI GKGCMC 2 cut(s) 474, 2828
BalI TGGCCA 2 cut(s) 453, 518
BamHI GGATCC 2 cut(s) 2689, 2761
BanI GGYRCC 4 cut(s) 157, 905, 1646, 2578
BanII GRGCYC 3 cut(s) 474, 836, 1479
BarI GAAGNNNNNNTAC 4 cut(s) 747, 779, 898, 930
BbsI GAAGAC 1 cut(s) 1335
Bbv12I GWGCWC 3 cut(s) 836, 1479, 2828
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 7 cut(s) 44, 1819, 1832, 2249, 2286, 2825, 3060
BceAI ACGGC 4 cut(s) 226, 1634, 1808, 1822
BciT130I CCWGG 6 cut(s) 1206, 1503, 1526, 1797, 1881, 3076
BciVI GTATCC 1 cut(s) 27
BclI TGATCA 1 cut(s) 2320
BcnI CCSGG 5 cut(s) 162, 1472, 2551, 2552, 3042
BcoDI GTCTC 4 cut(s) 804, 1565, 2659, 2880
BfaI CTAG 3 cut(s) 1613, 2243, 2799
BfmI CTRYAG 3 cut(s) 190, 578, 1086
BfrI CTTAAG 2 cut(s) 497, 941
BfuI GTATCC 1 cut(s) 27
BisI GCNGC 7 cut(s) 58, 1821, 1833, 2263, 2275, 2814, 3049
BlsI GCNGC 7 cut(s) 59, 1822, 1834, 2264, 2276, 2815, 3050
Bme18I GGWCC 4 cut(s) 1049, 1286, 1343, 1541
BmeT110I CYCGRG 1 cut(s) 2550
BmgBI CACGTC 1 cut(s) 1304
BmiI GGNNCC 7 cut(s) 159, 472, 907, 1648, 2580, 2691, 2763
BmrI ACTGGG 2 cut(s) 1120, 1908
BmsI GCATC 6 cut(s) 1312, 2183, 2249, 2274, 2464, 3014
BmuI ACTGGG 2 cut(s) 1120, 1908
BpiI GAAGAC 1 cut(s) 1335
BplI GAGNNNNNCTC 4 cut(s) 823, 855, 2802, 2834
BpmI CTGGAG 4 cut(s) 537, 1205, 1863, 2904
Bpu10I CCTNAGC 1 cut(s) 53
Bpu14I TTCGAA 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 1553
BpuMI CCSGG 5 cut(s) 162, 1472, 2551, 2552, 3042
Bsa29I ATCGAT 1 cut(s) 378
BsaBI GATNNNNATC 1 cut(s) 2076
BsaI GGTCTC 1 cut(s) 2659
BsaWI WCCGGW 5 cut(s) 154, 539, 1046, 1288, 1454
BsaXI ACNNNNNCTCC 4 cut(s) 535, 565, 2628, 2658
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 194, 560, 683, 1796, 2323, 2416, 2749
Bse118I RCCGGY 2 cut(s) 154, 406
Bse1I ACTGG 8 cut(s) 645, 907, 954, 1126, 1365, 1903, 2363, 2514
Bse3DI GCAATG 4 cut(s) 1396, 1556, 2880, 3058
Bse8I GATNNNNATC 1 cut(s) 2076
BseAI TCCGGA 2 cut(s) 539, 1046
BseBI CCWGG 6 cut(s) 1206, 1503, 1526, 1797, 1881, 3076
BseCI ATCGAT 1 cut(s) 378
BseGI GGATG 6 cut(s) 474, 909, 1016, 2264, 2992, 3005
BseJI GATNNNNATC 1 cut(s) 2076
BseLI CCNNNNNNNGG 8 cut(s) 161, 194, 560, 683, 1796, 2323, 2416, 2749
BseMI GCAATG 4 cut(s) 1396, 1556, 2880, 3058
BseMII CTCAG 9 cut(s) 67, 290, 622, 696, 947, 1347, 1502, 2040, 2671
BseNI ACTGG 8 cut(s) 645, 907, 954, 1126, 1365, 1903, 2363, 2514
BseRI GAGGAG 4 cut(s) 826, 844, 1987, 2432
BseSI GKGCMC 2 cut(s) 474, 2828
BseXI GCAGC 7 cut(s) 44, 1819, 1832, 2249, 2286, 2825, 3060
BseYI CCCAGC 1 cut(s) 241
Bsh1236I CGCG 1 cut(s) 2865
Bsh1285I CGRYCG 1 cut(s) 1424
BshNI GGYRCC 4 cut(s) 157, 905, 1646, 2578
BshTI ACCGGT 1 cut(s) 154
BshVI ATCGAT 1 cut(s) 378
BsiEI CGRYCG 1 cut(s) 1424
BsiHKAI GWGCWC 3 cut(s) 836, 1479, 2828
BsiHKCI CYCGRG 1 cut(s) 2550
BslFI GGGAC 5 cut(s) 158, 1314, 1483, 1789, 2575
BslI CCNNNNNNNGG 8 cut(s) 161, 194, 560, 683, 1796, 2323, 2416, 2749
BsmAI GTCTC 4 cut(s) 804, 1565, 2659, 2880
BsmBI CGTCTC 1 cut(s) 1565
BsmFI GGGAC 5 cut(s) 158, 1314, 1483, 1789, 2575
BsmI GAATGC 2 cut(s) 410, 1590
Bso31I GGTCTC 1 cut(s) 2659
BsoBI CYCGRG 1 cut(s) 2550
Bsp119I TTCGAA 1 cut(s) 753
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 4 cut(s) 474, 836, 1479, 2828
Bsp13I TCCGGA 2 cut(s) 539, 1046
Bsp1407I TGTACA 3 cut(s) 3, 315, 2437
Bsp19I CCATGG 1 cut(s) 823
BspACI CCGC 1 cut(s) 1651
BspCNI CTCAG 9 cut(s) 66, 291, 621, 695, 948, 1346, 1503, 2041, 2670
BspDI ATCGAT 1 cut(s) 378
BspEI TCCGGA 2 cut(s) 539, 1046
BspFNI CGCG 1 cut(s) 2865
BspHI TCATGA 1 cut(s) 2413
BspLI GGNNCC 7 cut(s) 159, 472, 907, 1648, 2580, 2691, 2763
BspMAI CTGCAG 1 cut(s) 194
BspQI GCTCTTC 2 cut(s) 1484, 1883
BspT104I TTCGAA 1 cut(s) 753
BspT107I GGYRCC 4 cut(s) 157, 905, 1646, 2578
BspTI CTTAAG 2 cut(s) 497, 941
BspTNI GGTCTC 1 cut(s) 2659
BsrDI GCAATG 4 cut(s) 1396, 1556, 2880, 3058
BsrFI RCCGGY 2 cut(s) 154, 406
BsrGI TGTACA 3 cut(s) 3, 315, 2437
BsrI ACTGG 8 cut(s) 645, 907, 954, 1126, 1365, 1903, 2363, 2514
BssAI RCCGGY 2 cut(s) 154, 406
BssT1I CCWWGG 6 cut(s) 480, 823, 1677, 2182, 2429, 3009
Bst2UI CCWGG 6 cut(s) 1206, 1503, 1526, 1797, 1881, 3076
Bst4CI ACNGT 6 cut(s) 260, 674, 1280, 1552, 1855, 2905
Bst6I CTCTTC 6 cut(s) 1059, 1206, 1484, 1653, 1883, 3130
BstAFI CTTAAG 2 cut(s) 497, 941
BstAPI GCANNNNNTGC 1 cut(s) 3062
BstAUI TGTACA 3 cut(s) 3, 315, 2437
BstBI TTCGAA 1 cut(s) 753
BstC8I GCNNGC 6 cut(s) 309, 516, 2279, 2828, 3123, 3127
BstDSI CCRYGG 1 cut(s) 823
BstEII GGTNACC 2 cut(s) 398, 1132
BstENI CCTNNNNNAGG 4 cut(s) 681, 1794, 2321, 2414
BstF5I GGATG 6 cut(s) 474, 909, 1016, 2264, 2992, 3005
BstFNI CGCG 1 cut(s) 2865
BstHHI GCGC 3 cut(s) 1590, 2283, 3091
BstMAI GTCTC 4 cut(s) 804, 1565, 2659, 2880
BstMCI CGRYCG 1 cut(s) 1424
BstMWI GCNNNNNNNGC 9 cut(s) 54, 89, 511, 1068, 1594, 1829, 2271, 2280, 3062
BstNI CCWGG 6 cut(s) 1206, 1503, 1526, 1797, 1881, 3076
BstNSI RCATGY 1 cut(s) 285
BstPI GGTNACC 2 cut(s) 398, 1132
BstSFI CTRYAG 3 cut(s) 190, 578, 1086
BstSLI GKGCMC 2 cut(s) 474, 2828
BstUI CGCG 1 cut(s) 2865
BstV1I GCAGC 7 cut(s) 44, 1819, 1832, 2249, 2286, 2825, 3060
BstV2I GAAGAC 1 cut(s) 1335
BstX2I RGATCY 5 cut(s) 197, 952, 2652, 2689, 2761
BstYI RGATCY 5 cut(s) 197, 952, 2652, 2689, 2761
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 27
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 823
BtrI CACGTC 1 cut(s) 1304
BtsCI GGATG 6 cut(s) 474, 909, 1016, 2264, 2992, 3005
BtsI GCAGTG 2 cut(s) 199, 3116
Cac8I GCNNGC 6 cut(s) 309, 516, 2279, 2828, 3123, 3127
CaiI CAGNNNCTG 3 cut(s) 1859, 1925, 2903
CciI TCATGA 1 cut(s) 2413
CfoI GCGC 3 cut(s) 1590, 2283, 3091
Cfr10I RCCGGY 2 cut(s) 154, 406
Cfr9I CCCGGG 1 cut(s) 2550
ClaI ATCGAT 1 cut(s) 378
CsiI ACCWGGT 1 cut(s) 1795
Csp6I GTAC 7 cut(s) 4, 316, 648, 906, 1647, 2127, 2438
CspAI ACCGGT 1 cut(s) 154
CspCI CAANNNNNGTGG 4 cut(s) 1264, 1299, 3040, 3075
CviQI GTAC 7 cut(s) 4, 316, 648, 906, 1647, 2127, 2438
DraI TTTAAA 2 cut(s) 879, 2944
EaeI YGGCCR 2 cut(s) 451, 516
Eam1104I CTCTTC 6 cut(s) 1059, 1206, 1484, 1653, 1883, 3130
EarI CTCTTC 6 cut(s) 1059, 1206, 1484, 1653, 1883, 3130
Ecl136II GAGCTC 2 cut(s) 834, 1477
Eco130I CCWWGG 6 cut(s) 480, 823, 1677, 2182, 2429, 3009
Eco24I GRGCYC 3 cut(s) 474, 836, 1479
Eco31I GGTCTC 1 cut(s) 2659
Eco32I GATATC 2 cut(s) 359, 1633
Eco47I GGWCC 4 cut(s) 1049, 1286, 1343, 1541
Eco53kI GAGCTC 2 cut(s) 834, 1477
Eco57I CTGAAG 6 cut(s) 129, 1064, 1347, 1428, 1821, 2088
Eco88I CYCGRG 1 cut(s) 2550
Eco91I GGTNACC 2 cut(s) 398, 1132
EcoICRI GAGCTC 2 cut(s) 834, 1477
EcoNI CCTNNNNNAGG 4 cut(s) 681, 1794, 2321, 2414
EcoO109I RGGNCCY 1 cut(s) 2021
EcoO65I GGTNACC 2 cut(s) 398, 1132
EcoRII CCWGG 6 cut(s) 1204, 1501, 1524, 1795, 1879, 3074
EcoRV GATATC 2 cut(s) 359, 1633
EcoT14I CCWWGG 6 cut(s) 480, 823, 1677, 2182, 2429, 3009
EcoT22I ATGCAT 1 cut(s) 2593
EcoT38I GRGCYC 3 cut(s) 474, 836, 1479
ErhI CCWWGG 6 cut(s) 480, 823, 1677, 2182, 2429, 3009
Esp3I CGTCTC 1 cut(s) 1565
FalI AAGNNNNNCTT 2 cut(s) 1995, 2027
FaqI GGGAC 5 cut(s) 158, 1314, 1483, 1789, 2575
FauI CCCGC 1 cut(s) 1658
FbaI TGATCA 1 cut(s) 2320
Fnu4HI GCNGC 7 cut(s) 58, 1821, 1833, 2263, 2275, 2814, 3049
FokI GGATG 6 cut(s) 461, 896, 1003, 2271, 2992, 2999
FriOI GRGCYC 3 cut(s) 474, 836, 1479
Fsp4HI GCNGC 7 cut(s) 58, 1821, 1833, 2263, 2275, 2814, 3049
FspBI CTAG 3 cut(s) 1613, 2243, 2799
GlaI GCGC 3 cut(s) 1589, 2282, 3090
GluI GCNGC 7 cut(s) 58, 1821, 1833, 2263, 2275, 2814, 3049
GsaI CCCAGC 1 cut(s) 245
GsuI CTGGAG 4 cut(s) 537, 1205, 1863, 2904
HhaI GCGC 3 cut(s) 1590, 2283, 3091
Hin6I GCGC 3 cut(s) 1588, 2281, 3089
HinP1I GCGC 3 cut(s) 1588, 2281, 3089
HincII GTYRAC 2 cut(s) 1165, 1696
HindII GTYRAC 2 cut(s) 1165, 1696
HindIII AAGCTT 2 cut(s) 1117, 2516
HinfI GANTC 9 cut(s) 606, 1331, 1960, 2191, 2239, 2370, 2420, 2637, 2725
HpaI GTTAAC 1 cut(s) 1696
HphI GGTGA 9 cut(s) 283, 392, 626, 634, 1144, 1444, 1811, 2171, 2829
Hpy166II GTNNAC 8 cut(s) 605, 1165, 1286, 1417, 1696, 2129, 2826, 3103
Hpy8I GTNNAC 8 cut(s) 605, 1165, 1286, 1417, 1696, 2129, 2826, 3103
HpyCH4III ACNGT 6 cut(s) 260, 674, 1280, 1552, 1855, 2905
HpyCH4IV ACGT 3 cut(s) 1303, 1698, 3105
HpyF10VI GCNNNNNNNGC 9 cut(s) 54, 89, 511, 1068, 1594, 1829, 2271, 2280, 3062
HpySE526I ACGT 3 cut(s) 1303, 1698, 3105
HspAI GCGC 3 cut(s) 1588, 2281, 3089
Kpn2I TCCGGA 2 cut(s) 539, 1046
KpnI GGTACC 2 cut(s) 909, 1650
Ksp22I TGATCA 1 cut(s) 2320
KspAI GTTAAC 1 cut(s) 1696
LguI GCTCTTC 2 cut(s) 1484, 1883
LmnI GCTCC 4 cut(s) 502, 831, 839, 1474
Lsp1109I GCAGC 7 cut(s) 44, 1819, 1832, 2249, 2286, 2825, 3060
LweI GCATC 6 cut(s) 1312, 2183, 2249, 2274, 2464, 3014
MabI ACCWGGT 1 cut(s) 1795
MaeI CTAG 3 cut(s) 1613, 2243, 2799
MaeII ACGT 3 cut(s) 1303, 1698, 3105
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 5 cut(s) 197, 952, 2652, 2689, 2761
MhlI GDGCHC 4 cut(s) 474, 836, 1479, 2828
MlsI TGGCCA 2 cut(s) 453, 518
MluI ACGCGT 1 cut(s) 2863
MluNI TGGCCA 2 cut(s) 453, 518
MlyI GAGTC 6 cut(s) 600, 1325, 2200, 2429, 2646, 2719
MmeI TCCRAC 1 cut(s) 1909
Mox20I TGGCCA 2 cut(s) 453, 518
Mph1103I ATGCAT 1 cut(s) 2593
MroI TCCGGA 2 cut(s) 539, 1046
MroXI GAANNNNTTC 4 cut(s) 389, 749, 1715, 2007
MscI TGGCCA 2 cut(s) 453, 518
MseI TTAA 8 cut(s) 498, 878, 896, 942, 1695, 1707, 2076, 2943
MslI CAYNNNNRTG 3 cut(s) 2445, 2736, 2906
Msp20I TGGCCA 2 cut(s) 453, 518
MspA1I CMGCKG 3 cut(s) 57, 73, 600
MspCI CTTAAG 2 cut(s) 497, 941
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 2 cut(s) 410, 1590
MvaI CCWGG 6 cut(s) 1206, 1503, 1526, 1797, 1881, 3076
MvnI CGCG 1 cut(s) 2865
MwoI GCNNNNNNNGC 9 cut(s) 54, 89, 511, 1068, 1594, 1829, 2271, 2280, 3062
NciI CCSGG 5 cut(s) 162, 1472, 2551, 2552, 3042
NcoI CCATGG 1 cut(s) 823
NlaIV GGNNCC 7 cut(s) 159, 472, 907, 1648, 2580, 2691, 2763
NmuCI GTSAC 6 cut(s) 398, 1132, 1450, 1465, 2859, 3106
NsiI ATGCAT 1 cut(s) 2593
NspI RCATGY 1 cut(s) 285
NspV TTCGAA 1 cut(s) 753
PagI TCATGA 1 cut(s) 2413
PciSI GCTCTTC 2 cut(s) 1484, 1883
PctI GAATGC 2 cut(s) 410, 1590
PdmI GAANNNNTTC 4 cut(s) 389, 749, 1715, 2007
PfeI GAWTC 3 cut(s) 1960, 2239, 2370
PflMI CCANNNNNTGG 2 cut(s) 560, 2749
PfoI TCCNGGA 2 cut(s) 1204, 1879
PinAI ACCGGT 1 cut(s) 154
PkrI GCNGC 7 cut(s) 59, 1822, 1834, 2264, 2276, 2815, 3050
Ple19I CGATCG 1 cut(s) 1424
PleI GAGTC 6 cut(s) 600, 1325, 2199, 2428, 2645, 2719
PpsI GAGTC 6 cut(s) 600, 1325, 2199, 2428, 2645, 2719
PshBI ATTAAT 1 cut(s) 896
PsiI TTATAA 2 cut(s) 2121, 2937
Psp124BI GAGCTC 2 cut(s) 836, 1479
Psp6I CCWGG 6 cut(s) 1204, 1501, 1524, 1795, 1879, 3074
PspEI GGTNACC 2 cut(s) 398, 1132
PspFI CCCAGC 1 cut(s) 241
PspGI CCWGG 6 cut(s) 1204, 1501, 1524, 1795, 1879, 3074
PspN4I GGNNCC 7 cut(s) 159, 472, 907, 1648, 2580, 2691, 2763
PspOMI GGGCCC 1 cut(s) 470
PstI CTGCAG 1 cut(s) 194
PstNI CAGNNNCTG 3 cut(s) 1859, 1925, 2903
PsuI RGATCY 5 cut(s) 197, 952, 2652, 2689, 2761
PvuI CGATCG 1 cut(s) 1424
PvuII CAGCTG 3 cut(s) 57, 73, 600
RsaI GTAC 7 cut(s) 5, 317, 649, 907, 1648, 2128, 2439
RsaNI GTAC 7 cut(s) 4, 316, 648, 906, 1647, 2127, 2438
RseI CAYNNNNRTG 3 cut(s) 2445, 2736, 2906
SacI GAGCTC 2 cut(s) 836, 1479
SapI GCTCTTC 2 cut(s) 1484, 1883
SaqAI TTAA 8 cut(s) 498, 878, 896, 942, 1695, 1707, 2076, 2943
SatI GCNGC 7 cut(s) 58, 1821, 1833, 2263, 2275, 2814, 3049
SchI GAGTC 6 cut(s) 600, 1325, 2200, 2429, 2646, 2719
SduI GDGCHC 4 cut(s) 474, 836, 1479, 2828
SexAI ACCWGGT 1 cut(s) 1795
SfaNI GCATC 6 cut(s) 1312, 2183, 2249, 2274, 2464, 3014
SfcI CTRYAG 3 cut(s) 190, 578, 1086
SfuI TTCGAA 1 cut(s) 753
SinI GGWCC 4 cut(s) 1049, 1286, 1343, 1541
SmaI CCCGGG 1 cut(s) 2552
SmiMI CAYNNNNRTG 3 cut(s) 2445, 2736, 2906
SmlI CTYRAG 3 cut(s) 497, 941, 1532
SmoI CTYRAG 3 cut(s) 497, 941, 1532
SsiI CCGC 1 cut(s) 1651
SspI AATATT 4 cut(s) 132, 1716, 1953, 2464
SspMI CTAG 3 cut(s) 1613, 2243, 2799
SstI GAGCTC 2 cut(s) 836, 1479
StyI CCWWGG 6 cut(s) 480, 823, 1677, 2182, 2429, 3009
TaaI ACNGT 6 cut(s) 260, 674, 1280, 1552, 1855, 2905
TaiI ACGT 3 cut(s) 1306, 1701, 3108
TatI WGTACW 3 cut(s) 3, 315, 2437
TfiI GAWTC 3 cut(s) 1960, 2239, 2370
Tru1I TTAA 8 cut(s) 498, 878, 896, 942, 1695, 1707, 2076, 2943
Tru9I TTAA 8 cut(s) 498, 878, 896, 942, 1695, 1707, 2076, 2943
TseFI GTSAC 6 cut(s) 398, 1132, 1450, 1465, 2859, 3106
TseI GCWGC 7 cut(s) 57, 1820, 1832, 2262, 2274, 2813, 3048
Tsp45I GTSAC 6 cut(s) 398, 1132, 1450, 1465, 2859, 3106
TspGWI ACGGA 1 cut(s) 1589
TspMI CCCGGG 1 cut(s) 2550
Van91I CCANNNNNTGG 2 cut(s) 560, 2749
Vha464I CTTAAG 2 cut(s) 497, 941
VneI GTGCAC 1 cut(s) 2824
VpaK11BI GGWCC 4 cut(s) 1049, 1286, 1343, 1541
VspI ATTAAT 1 cut(s) 896
XagI CCTNNNNNAGG 4 cut(s) 681, 1794, 2321, 2414
XapI RAATTY 4 cut(s) 585, 618, 1228, 1749
XceI RCATGY 1 cut(s) 285
XmaI CCCGGG 1 cut(s) 2550
XmnI GAANNNNTTC 4 cut(s) 389, 749, 1715, 2007
XspI CTAG 3 cut(s) 1613, 2243, 2799
Zsp2I ATGCAT 1 cut(s) 2593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.