Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 686 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 182 |
| AccI | GTMKAC | 2 cut(s) | 1209, 1977 |
| AccII | CGCG | 1 cut(s) | 2082 |
| AciI | CCGC | 4 cut(s) | 191, 429, 1073, 1878 |
| AclWI | GGATC | 6 cut(s) | 83, 809, 822, 1001, 1157, 1889 |
| AcoI | YGGCCR | 1 cut(s) | 141 |
| AcsI | RAATTY | 6 cut(s) | 463, 1465, 1888, 1994, 2156, 2192 |
| AcuI | CTGAAG | 3 cut(s) | 117, 936, 2239 |
| AcyI | GRCGYC | 1 cut(s) | 683 |
| AfaI | GTAC | 7 cut(s) | 1297, 1345, 1446, 1656, 1765, 2209, 2281 |
| AfeI | AGCGCT | 1 cut(s) | 108 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 182, 377, 760, 1292, 1340 |
| AflIII | ACRYGT | 1 cut(s) | 1583 |
| AjnI | CCWGG | 4 cut(s) | 768, 783, 1333, 2134 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 311, 343 |
| Alw21I | GWGCWC | 1 cut(s) | 2045 |
| Alw26I | GTCTC | 4 cut(s) | 690, 773, 980, 2082 |
| Alw44I | GTGCAC | 1 cut(s) | 2041 |
| AlwI | GGATC | 6 cut(s) | 83, 809, 822, 1001, 1157, 1889 |
| Ama87I | CYCGRG | 1 cut(s) | 1767 |
| Aor51HI | AGCGCT | 1 cut(s) | 108 |
| AoxI | GGCC | 2 cut(s) | 141, 1883 |
| ApaLI | GTGCAC | 1 cut(s) | 2041 |
| ApeKI | GCWGC | 7 cut(s) | 29, 32, 35, 42, 619, 1278, 2120 |
| ApoI | RAATTY | 6 cut(s) | 463, 1465, 1888, 1994, 2156, 2192 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2141, 2173 |
| AspLEI | GCGC | 1 cut(s) | 109 |
| AspS9I | GGNCC | 4 cut(s) | 215, 789, 1091, 1304 |
| AsuII | TTCGAA | 1 cut(s) | 1648 |
| AvaI | CYCGRG | 1 cut(s) | 1767 |
| AvaII | GGWCC | 4 cut(s) | 215, 789, 1091, 1304 |
| BaeGI | GKGCMC | 1 cut(s) | 2045 |
| BamHI | GGATCC | 1 cut(s) | 814 |
| BanII | GRGCYC | 1 cut(s) | 1086 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 564, 596 |
| BbsI | GAAGAC | 2 cut(s) | 936, 1838 |
| Bbv12I | GWGCWC | 1 cut(s) | 2045 |
| BbvCI | CCTCAGC | 1 cut(s) | 704 |
| BbvI | GCAGC | 7 cut(s) | 16, 44, 47, 54, 606, 1265, 2107 |
| BccI | CCATC | 6 cut(s) | 592, 1119, 1328, 1349, 2195, 2348 |
| BceAI | ACGGC | 3 cut(s) | 156, 1355, 1514 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1826, 1860 |
| BciT130I | CCWGG | 4 cut(s) | 770, 785, 1335, 2136 |
| BciVI | GTATCC | 1 cut(s) | 501 |
| BclI | TGATCA | 1 cut(s) | 1537 |
| BcoDI | GTCTC | 4 cut(s) | 690, 773, 980, 2082 |
| BfaI | CTAG | 8 cut(s) | 689, 990, 1460, 1611, 1973, 1983, 2222, 2241 |
| BfmI | CTRYAG | 2 cut(s) | 30, 40 |
| BfoI | RGCGCY | 1 cut(s) | 110 |
| BfuI | GTATCC | 1 cut(s) | 501 |
| BisI | GCNGC | 7 cut(s) | 30, 33, 36, 43, 620, 1279, 2121 |
| BlsI | GCNGC | 7 cut(s) | 31, 34, 37, 44, 621, 1280, 2122 |
| BmcAI | AGTACT | 2 cut(s) | 1765, 2209 |
| Bme1390I | CCNGG | 4 cut(s) | 770, 785, 1335, 2136 |
| Bme18I | GGWCC | 4 cut(s) | 215, 789, 1091, 1304 |
| BmeT110I | CYCGRG | 1 cut(s) | 1767 |
| BmgT120I | GGNCC | 4 cut(s) | 215, 789, 1091, 1304 |
| BmiI | GGNNCC | 5 cut(s) | 577, 790, 816, 1409, 1502 |
| BmrFI | CCNGG | 4 cut(s) | 770, 785, 1335, 2136 |
| BmsI | GCATC | 4 cut(s) | 103, 236, 1397, 1576 |
| BpiI | GAAGAC | 2 cut(s) | 936, 1838 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 994, 1026 |
| BpmI | CTGGAG | 1 cut(s) | 1541 |
| Bpu10I | CCTNAGC | 2 cut(s) | 704, 1218 |
| Bpu14I | TTCGAA | 1 cut(s) | 1648 |
| BpuEI | CTTGAG | 2 cut(s) | 568, 1208 |
| Bsa29I | ATCGAT | 1 cut(s) | 230 |
| BsaHI | GRCGYC | 1 cut(s) | 683 |
| BsaI | GGTCTC | 1 cut(s) | 773 |
| BsaJI | CCNNGG | 5 cut(s) | 783, 784, 1087, 1399, 1485 |
| BsaWI | WCCGGW | 3 cut(s) | 212, 511, 1798 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 182, 377, 760, 1292, 1340 |
| Bse1I | ACTGG | 7 cut(s) | 172, 505, 792, 818, 1411, 2156, 2215 |
| Bse3DI | GCAATG | 2 cut(s) | 161, 1701 |
| BseBI | CCWGG | 4 cut(s) | 770, 785, 1335, 2136 |
| BseCI | ATCGAT | 1 cut(s) | 230 |
| BseDI | CCNNGG | 5 cut(s) | 783, 784, 1087, 1399, 1485 |
| BseGI | GGATG | 3 cut(s) | 118, 879, 1391 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 182, 377, 760, 1292, 1340 |
| BseMI | GCAATG | 2 cut(s) | 161, 1701 |
| BseMII | CTCAG | 6 cut(s) | 363, 695, 1071, 1180, 1232, 1413 |
| BseNI | ACTGG | 7 cut(s) | 172, 505, 792, 818, 1411, 2156, 2215 |
| BseRI | GAGGAG | 1 cut(s) | 1304 |
| BseSI | GKGCMC | 1 cut(s) | 2045 |
| BseXI | GCAGC | 7 cut(s) | 16, 44, 47, 54, 606, 1265, 2107 |
| BsgI | GTGCAG | 1 cut(s) | 1465 |
| Bsh1236I | CGCG | 1 cut(s) | 2082 |
| BshFI | GGCC | 2 cut(s) | 143, 1885 |
| BshVI | ATCGAT | 1 cut(s) | 230 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2045 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1767 |
| BsiSI | CCGG | 3 cut(s) | 213, 512, 1799 |
| BslFI | GGGAC | 4 cut(s) | 282, 355, 802, 1285 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 182, 377, 760, 1292, 1340 |
| BsmAI | GTCTC | 4 cut(s) | 690, 773, 980, 2082 |
| BsmBI | CGTCTC | 2 cut(s) | 690, 980 |
| BsmFI | GGGAC | 4 cut(s) | 282, 355, 802, 1285 |
| BsmI | GAATGC | 2 cut(s) | 2097, 2302 |
| BsnI | GGCC | 2 cut(s) | 143, 1885 |
| Bso31I | GGTCTC | 1 cut(s) | 773 |
| BsoBI | CYCGRG | 1 cut(s) | 1767 |
| Bsp119I | TTCGAA | 1 cut(s) | 1648 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1086, 2045 |
| Bsp1407I | TGTACA | 1 cut(s) | 1654 |
| Bsp143I | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| BspACI | CCGC | 4 cut(s) | 191, 429, 1073, 1878 |
| BspANI | GGCC | 2 cut(s) | 143, 1885 |
| BspCNI | CTCAG | 6 cut(s) | 364, 696, 1072, 1179, 1231, 1414 |
| BspDI | ATCGAT | 1 cut(s) | 230 |
| BspFNI | CGCG | 1 cut(s) | 2082 |
| BspHI | TCATGA | 1 cut(s) | 1630 |
| BspLI | GGNNCC | 5 cut(s) | 577, 790, 816, 1409, 1502 |
| BspMAI | CTGCAG | 2 cut(s) | 34, 44 |
| BspPI | GGATC | 6 cut(s) | 83, 809, 822, 1001, 1157, 1889 |
| BspT104I | TTCGAA | 1 cut(s) | 1648 |
| BspTNI | GGTCTC | 1 cut(s) | 773 |
| BsrDI | GCAATG | 2 cut(s) | 161, 1701 |
| BsrGI | TGTACA | 1 cut(s) | 1654 |
| BsrI | ACTGG | 7 cut(s) | 172, 505, 792, 818, 1411, 2156, 2215 |
| BssECI | CCNNGG | 5 cut(s) | 783, 784, 1087, 1399, 1485 |
| BssMI | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| BssNAI | GTATAC | 1 cut(s) | 1210 |
| BssNI | GRCGYC | 1 cut(s) | 683 |
| BssT1I | CCWWGG | 2 cut(s) | 1087, 1399 |
| Bst1107I | GTATAC | 1 cut(s) | 1210 |
| Bst2UI | CCWGG | 4 cut(s) | 770, 785, 1335, 2136 |
| Bst4CI | ACNGT | 3 cut(s) | 594, 1063, 1207 |
| Bst6I | CTCTTC | 4 cut(s) | 661, 983, 1354, 2331 |
| BstACI | GRCGYC | 1 cut(s) | 683 |
| BstAUI | TGTACA | 1 cut(s) | 1654 |
| BstBI | TTCGAA | 1 cut(s) | 1648 |
| BstC8I | GCNNGC | 2 cut(s) | 40, 2097 |
| BstDEI | CTNAG | 7 cut(s) | 372, 425, 704, 1080, 1166, 1218, 1422 |
| BstEII | GGTNACC | 1 cut(s) | 170 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 375, 1338 |
| BstF5I | GGATG | 3 cut(s) | 118, 879, 1391 |
| BstFNI | CGCG | 1 cut(s) | 2082 |
| BstH2I | RGCGCY | 1 cut(s) | 110 |
| BstHHI | GCGC | 1 cut(s) | 109 |
| BstKTI | GATC | 8 cut(s) | 91, 817, 1009, 1152, 1540, 1897, 1972, 2088 |
| BstMAI | GTCTC | 4 cut(s) | 690, 773, 980, 2082 |
| BstMBI | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 35, 113, 1325 |
| BstNI | CCWGG | 4 cut(s) | 770, 785, 1335, 2136 |
| BstPI | GGTNACC | 1 cut(s) | 170 |
| BstSCI | CCNGG | 4 cut(s) | 768, 783, 1333, 2134 |
| BstSFI | CTRYAG | 2 cut(s) | 30, 40 |
| BstSLI | GKGCMC | 1 cut(s) | 2045 |
| BstUI | CGCG | 1 cut(s) | 2082 |
| BstV1I | GCAGC | 7 cut(s) | 16, 44, 47, 54, 606, 1265, 2107 |
| BstV2I | GAAGAC | 2 cut(s) | 936, 1838 |
| BstX2I | RGATCY | 3 cut(s) | 88, 814, 1149 |
| BstYI | RGATCY | 3 cut(s) | 88, 814, 1149 |
| BstZ17I | GTATAC | 1 cut(s) | 1210 |
| Bsu15I | ATCGAT | 1 cut(s) | 230 |
| BsuI | GTATCC | 1 cut(s) | 501 |
| BsuRI | GGCC | 2 cut(s) | 143, 1885 |
| BsuTUI | ATCGAT | 1 cut(s) | 230 |
| BtsCI | GGATG | 3 cut(s) | 118, 879, 1391 |
| BtsI | GCAGTG | 1 cut(s) | 187 |
| BtsIMutI | CAGTG | 6 cut(s) | 187, 498, 590, 799, 1077, 1330 |
| Cac8I | GCNNGC | 2 cut(s) | 40, 2097 |
| CciI | TCATGA | 1 cut(s) | 1630 |
| CfoI | GCGC | 1 cut(s) | 109 |
| Cfr13I | GGNCC | 4 cut(s) | 215, 789, 1091, 1304 |
| ClaI | ATCGAT | 1 cut(s) | 230 |
| CsiI | ACCWGGT | 1 cut(s) | 1333 |
| Csp6I | GTAC | 7 cut(s) | 1296, 1344, 1445, 1655, 1764, 2208, 2280 |
| CviAII | CATG | 7 cut(s) | 177, 930, 966, 1017, 1631, 1759, 1949 |
| CviQI | GTAC | 7 cut(s) | 1296, 1344, 1445, 1655, 1764, 2208, 2280 |
| DdeI | CTNAG | 7 cut(s) | 372, 425, 704, 1080, 1166, 1218, 1422 |
| DpnI | GATC | 8 cut(s) | 90, 816, 1008, 1151, 1539, 1896, 1971, 2087 |
| DpnII | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| EaeI | YGGCCR | 1 cut(s) | 141 |
| Eam1104I | CTCTTC | 4 cut(s) | 661, 983, 1354, 2331 |
| EarI | CTCTTC | 4 cut(s) | 661, 983, 1354, 2331 |
| EciI | GGCGGA | 1 cut(s) | 1088 |
| Eco130I | CCWWGG | 2 cut(s) | 1087, 1399 |
| Eco24I | GRGCYC | 1 cut(s) | 1086 |
| Eco31I | GGTCTC | 1 cut(s) | 773 |
| Eco47I | GGWCC | 4 cut(s) | 215, 789, 1091, 1304 |
| Eco47III | AGCGCT | 1 cut(s) | 108 |
| Eco57I | CTGAAG | 3 cut(s) | 117, 936, 2239 |
| Eco88I | CYCGRG | 1 cut(s) | 1767 |
| Eco91I | GGTNACC | 1 cut(s) | 170 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 375, 1338 |
| EcoO65I | GGTNACC | 1 cut(s) | 170 |
| EcoRI | GAATTC | 1 cut(s) | 1994 |
| EcoRII | CCWGG | 4 cut(s) | 768, 783, 1333, 2134 |
| EcoT14I | CCWWGG | 2 cut(s) | 1087, 1399 |
| EcoT38I | GRGCYC | 1 cut(s) | 1086 |
| ErhI | CCWWGG | 2 cut(s) | 1087, 1399 |
| Esp3I | CGTCTC | 2 cut(s) | 690, 980 |
| FaeI | CATG | 7 cut(s) | 180, 933, 969, 1020, 1634, 1762, 1952 |
| FaqI | GGGAC | 4 cut(s) | 282, 355, 802, 1285 |
| FatI | CATG | 7 cut(s) | 176, 929, 965, 1016, 1630, 1758, 1948 |
| FauNDI | CATATG | 1 cut(s) | 1300 |
| FbaI | TGATCA | 1 cut(s) | 1537 |
| FblI | GTMKAC | 2 cut(s) | 1209, 1977 |
| Fnu4HI | GCNGC | 7 cut(s) | 30, 33, 36, 43, 620, 1279, 2121 |
| FokI | GGATG | 3 cut(s) | 125, 886, 1378 |
| FriOI | GRGCYC | 1 cut(s) | 1086 |
| Fsp4HI | GCNGC | 7 cut(s) | 30, 33, 36, 43, 620, 1279, 2121 |
| FspBI | CTAG | 8 cut(s) | 689, 990, 1460, 1611, 1973, 1983, 2222, 2241 |
| GlaI | GCGC | 1 cut(s) | 108 |
| GluI | GCNGC | 7 cut(s) | 30, 33, 36, 43, 620, 1279, 2121 |
| GsuI | CTGGAG | 1 cut(s) | 1541 |
| HaeII | RGCGCY | 1 cut(s) | 110 |
| HaeIII | GGCC | 2 cut(s) | 143, 1885 |
| HapII | CCGG | 3 cut(s) | 213, 512, 1799 |
| HhaI | GCGC | 1 cut(s) | 109 |
| Hin1I | GRCGYC | 1 cut(s) | 683 |
| Hin1II | CATG | 7 cut(s) | 180, 933, 969, 1020, 1634, 1762, 1952 |
| Hin6I | GCGC | 1 cut(s) | 107 |
| HinP1I | GCGC | 1 cut(s) | 107 |
| HincII | GTYRAC | 1 cut(s) | 855 |
| HindII | GTYRAC | 1 cut(s) | 855 |
| HindIII | AAGCTT | 2 cut(s) | 536, 823 |
| HinfI | GANTC | 9 cut(s) | 436, 604, 725, 941, 1476, 1531, 1637, 1691, 1979 |
| HpaII | CCGG | 3 cut(s) | 213, 512, 1799 |
| Hpy188I | TCNGA | 9 cut(s) | 97, 126, 724, 1169, 1231, 1717, 1894, 1993, 2090 |
| HpyAV | CCTTC | 7 cut(s) | 566, 744, 1398, 1637, 1663, 1901, 2322 |
| HpyCH4III | ACNGT | 3 cut(s) | 594, 1063, 1207 |
| HpyCH4IV | ACGT | 4 cut(s) | 683, 973, 1585, 1960 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 35, 113, 1325 |
| HpyF3I | CTNAG | 7 cut(s) | 372, 425, 704, 1080, 1166, 1218, 1422 |
| HpySE526I | ACGT | 4 cut(s) | 683, 973, 1585, 1960 |
| Hsp92I | GRCGYC | 1 cut(s) | 683 |
| Hsp92II | CATG | 7 cut(s) | 180, 933, 969, 1020, 1634, 1762, 1952 |
| HspAI | GCGC | 1 cut(s) | 107 |
| Ksp22I | TGATCA | 1 cut(s) | 1537 |
| Kzo9I | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| LmnI | GCTCC | 2 cut(s) | 1490, 1506 |
| Lsp1109I | GCAGC | 7 cut(s) | 16, 44, 47, 54, 606, 1265, 2107 |
| LweI | GCATC | 4 cut(s) | 103, 236, 1397, 1576 |
| MabI | ACCWGGT | 1 cut(s) | 1333 |
| MaeI | CTAG | 8 cut(s) | 689, 990, 1460, 1611, 1973, 1983, 2222, 2241 |
| MaeII | ACGT | 4 cut(s) | 683, 973, 1585, 1960 |
| MaeIII | GTNAC | 8 cut(s) | 136, 170, 1108, 1417, 1676, 2113, 2138, 2167 |
| MalI | GATC | 8 cut(s) | 90, 816, 1008, 1151, 1539, 1896, 1971, 2087 |
| MboI | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| MfeI | CAATTG | 2 cut(s) | 1379, 1782 |
| MflI | RGATCY | 3 cut(s) | 88, 814, 1149 |
| MhlI | GDGCHC | 2 cut(s) | 1086, 2045 |
| MlyI | GAGTC | 5 cut(s) | 598, 950, 1540, 1685, 1973 |
| MmeI | TCCRAC | 1 cut(s) | 762 |
| MseI | TTAA | 4 cut(s) | 386, 635, 698, 1619 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1662, 1740 |
| MspA1I | CMGCKG | 1 cut(s) | 1878 |
| MspI | CCGG | 3 cut(s) | 213, 512, 1799 |
| MspR9I | CCNGG | 4 cut(s) | 770, 785, 1335, 2136 |
| MunI | CAATTG | 2 cut(s) | 1379, 1782 |
| Mva1269I | GAATGC | 2 cut(s) | 2097, 2302 |
| MvaI | CCWGG | 4 cut(s) | 770, 785, 1335, 2136 |
| MvnI | CGCG | 1 cut(s) | 2082 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 35, 113, 1325 |
| NdeI | CATATG | 1 cut(s) | 1300 |
| NdeII | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| NlaIII | CATG | 7 cut(s) | 180, 933, 969, 1020, 1634, 1762, 1952 |
| NlaIV | GGNNCC | 5 cut(s) | 577, 790, 816, 1409, 1502 |
| NmuCI | GTSAC | 4 cut(s) | 170, 1108, 1417, 2113 |
| NspV | TTCGAA | 1 cut(s) | 1648 |
| PagI | TCATGA | 1 cut(s) | 1630 |
| PasI | CCCWGGG | 1 cut(s) | 784 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 146 |
| PctI | GAATGC | 2 cut(s) | 2097, 2302 |
| PfeI | GAWTC | 4 cut(s) | 436, 725, 1476, 1637 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 182 |
| PkrI | GCNGC | 7 cut(s) | 31, 34, 37, 44, 621, 1280, 2122 |
| PleI | GAGTC | 5 cut(s) | 598, 949, 1539, 1685, 1973 |
| PpsI | GAGTC | 5 cut(s) | 598, 949, 1539, 1685, 1973 |
| Psp6I | CCWGG | 4 cut(s) | 768, 783, 1333, 2134 |
| PspEI | GGTNACC | 1 cut(s) | 170 |
| PspGI | CCWGG | 4 cut(s) | 768, 783, 1333, 2134 |
| PspN4I | GGNNCC | 5 cut(s) | 577, 790, 816, 1409, 1502 |
| PspPI | GGNCC | 4 cut(s) | 215, 789, 1091, 1304 |
| PstI | CTGCAG | 2 cut(s) | 34, 44 |
| PsuI | RGATCY | 3 cut(s) | 88, 814, 1149 |
| RsaI | GTAC | 7 cut(s) | 1297, 1345, 1446, 1656, 1765, 2209, 2281 |
| RsaNI | GTAC | 7 cut(s) | 1296, 1344, 1445, 1655, 1764, 2208, 2280 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1662, 1740 |
| SaqAI | TTAA | 4 cut(s) | 386, 635, 698, 1619 |
| SatI | GCNGC | 7 cut(s) | 30, 33, 36, 43, 620, 1279, 2121 |
| Sau3AI | GATC | 8 cut(s) | 88, 814, 1006, 1149, 1537, 1894, 1969, 2085 |
| Sau96I | GGNCC | 4 cut(s) | 215, 789, 1091, 1304 |
| ScaI | AGTACT | 2 cut(s) | 1765, 2209 |
| SchI | GAGTC | 5 cut(s) | 598, 950, 1540, 1685, 1973 |
| ScrFI | CCNGG | 4 cut(s) | 770, 785, 1335, 2136 |
| SduI | GDGCHC | 2 cut(s) | 1086, 2045 |
| SexAI | ACCWGGT | 1 cut(s) | 1333 |
| SfaNI | GCATC | 4 cut(s) | 103, 236, 1397, 1576 |
| SfcI | CTRYAG | 2 cut(s) | 30, 40 |
| SfuI | TTCGAA | 1 cut(s) | 1648 |
| SinI | GGWCC | 4 cut(s) | 215, 789, 1091, 1304 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1662, 1740 |
| SmlI | CTYRAG | 2 cut(s) | 547, 1187 |
| SmoI | CTYRAG | 2 cut(s) | 547, 1187 |
| SsiI | CCGC | 4 cut(s) | 191, 429, 1073, 1878 |
| SspI | AATATT | 1 cut(s) | 718 |
| SspMI | CTAG | 8 cut(s) | 689, 990, 1460, 1611, 1973, 1983, 2222, 2241 |
| StyD4I | CCNGG | 4 cut(s) | 768, 783, 1333, 2134 |
| StyI | CCWWGG | 2 cut(s) | 1087, 1399 |
| TaaI | ACNGT | 3 cut(s) | 594, 1063, 1207 |
| TaiI | ACGT | 4 cut(s) | 686, 976, 1588, 1963 |
| TaqI | TCGA | 2 cut(s) | 230, 1648 |
| TaqII | GACCGA | 1 cut(s) | 1850 |
| TatI | WGTACW | 4 cut(s) | 1654, 1763, 2207, 2279 |
| TfiI | GAWTC | 4 cut(s) | 436, 725, 1476, 1637 |
| Tru1I | TTAA | 4 cut(s) | 386, 635, 698, 1619 |
| Tru9I | TTAA | 4 cut(s) | 386, 635, 698, 1619 |
| TscAI | CASTG | 6 cut(s) | 187, 505, 597, 799, 1084, 1330 |
| TseFI | GTSAC | 4 cut(s) | 170, 1108, 1417, 2113 |
| TseI | GCWGC | 7 cut(s) | 29, 32, 35, 42, 619, 1278, 2120 |
| Tsp45I | GTSAC | 4 cut(s) | 170, 1108, 1417, 2113 |
| TspRI | CASTG | 6 cut(s) | 187, 505, 597, 799, 1084, 1330 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 182 |
| VneI | GTGCAC | 1 cut(s) | 2041 |
| VpaK11BI | GGWCC | 4 cut(s) | 215, 789, 1091, 1304 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 375, 1338 |
| XapI | RAATTY | 6 cut(s) | 463, 1465, 1888, 1994, 2156, 2192 |
| XbaI | TCTAGA | 2 cut(s) | 688, 1982 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2192 |
| XmiI | GTMKAC | 2 cut(s) | 1209, 1977 |
| XspI | CTAG | 8 cut(s) | 689, 990, 1460, 1611, 1973, 1983, 2222, 2241 |
| ZraI | GACGTC | 1 cut(s) | 684 |
| ZrmI | AGTACT | 2 cut(s) | 1765, 2209 |