Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 162 |
| Acc65I | GGTACC | 1 cut(s) | 572 |
| AccB1I | GGYRCC | 1 cut(s) | 572 |
| AclI | AACGTT | 1 cut(s) | 291 |
| AclWI | GGATC | 1 cut(s) | 179 |
| AcsI | RAATTY | 2 cut(s) | 5, 153 |
| AfaI | GTAC | 2 cut(s) | 326, 574 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 568 |
| AgsI | TTSAA | 5 cut(s) | 10, 84, 112, 122, 352 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 240 |
| AjnI | CCWGG | 3 cut(s) | 35, 72, 531 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 459, 491 |
| AluBI | AGCT | 3 cut(s) | 248, 373, 473 |
| AluI | AGCT | 3 cut(s) | 248, 373, 473 |
| Alw21I | GWGCWC | 1 cut(s) | 97 |
| Alw26I | GTCTC | 2 cut(s) | 120, 227 |
| AlwI | GGATC | 1 cut(s) | 179 |
| ApeKI | GCWGC | 2 cut(s) | 312, 617 |
| ApoI | RAATTY | 2 cut(s) | 5, 153 |
| Asp718I | GGTACC | 1 cut(s) | 572 |
| AspLEI | GCGC | 1 cut(s) | 513 |
| AspS9I | GGNCC | 2 cut(s) | 185, 242 |
| AsuHPI | GGTGA | 1 cut(s) | 61 |
| AvaII | GGWCC | 2 cut(s) | 185, 242 |
| BanI | GGYRCC | 1 cut(s) | 572 |
| BbsI | GAAGAC | 1 cut(s) | 192 |
| Bbv12I | GWGCWC | 1 cut(s) | 97 |
| BbvI | GCAGC | 2 cut(s) | 324, 629 |
| BccI | CCATC | 3 cut(s) | 270, 559, 578 |
| BceAI | ACGGC | 2 cut(s) | 584, 656 |
| BciT130I | CCWGG | 3 cut(s) | 37, 74, 533 |
| BclI | TGATCA | 1 cut(s) | 766 |
| BcoDI | GTCTC | 2 cut(s) | 120, 227 |
| BfaI | CTAG | 3 cut(s) | 362, 479, 689 |
| BfmI | CTRYAG | 2 cut(s) | 455, 615 |
| BisI | GCNGC | 2 cut(s) | 313, 618 |
| BlsI | GCNGC | 2 cut(s) | 314, 619 |
| Bme1390I | CCNGG | 3 cut(s) | 37, 74, 533 |
| Bme18I | GGWCC | 2 cut(s) | 185, 242 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 240 |
| BmgT120I | GGNCC | 2 cut(s) | 185, 242 |
| BmiI | GGNNCC | 3 cut(s) | 186, 574, 731 |
| BmrFI | CCNGG | 3 cut(s) | 37, 74, 533 |
| BmsI | GCATC | 2 cut(s) | 538, 629 |
| BpiI | GAAGAC | 1 cut(s) | 192 |
| BpuEI | CTTGAG | 1 cut(s) | 449 |
| BsaI | GGTCTC | 1 cut(s) | 227 |
| BsaJI | CCNNGG | 3 cut(s) | 72, 238, 628 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 568 |
| Bse1I | ACTGG | 2 cut(s) | 310, 640 |
| BseBI | CCWGG | 3 cut(s) | 37, 74, 533 |
| BseDI | CCNNGG | 3 cut(s) | 72, 238, 628 |
| BseGI | GGATG | 1 cut(s) | 620 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 568 |
| BseMII | CTCAG | 5 cut(s) | 203, 335, 360, 642, 705 |
| BseNI | ACTGG | 2 cut(s) | 310, 640 |
| BseRI | GAGGAG | 1 cut(s) | 328 |
| BseXI | GCAGC | 2 cut(s) | 324, 629 |
| BsgI | GTGCAG | 1 cut(s) | 694 |
| BshNI | GGYRCC | 1 cut(s) | 572 |
| BsiHKAI | GWGCWC | 1 cut(s) | 97 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 568 |
| BsmAI | GTCTC | 2 cut(s) | 120, 227 |
| Bso31I | GGTCTC | 1 cut(s) | 227 |
| Bsp1286I | GDGCHC | 1 cut(s) | 97 |
| Bsp143I | GATC | 2 cut(s) | 171, 766 |
| BspCNI | CTCAG | 5 cut(s) | 202, 334, 361, 643, 706 |
| BspLI | GGNNCC | 3 cut(s) | 186, 574, 731 |
| BspMAI | CTGCAG | 1 cut(s) | 619 |
| BspPI | GGATC | 1 cut(s) | 179 |
| BspQI | GCTCTTC | 1 cut(s) | 102 |
| BspT107I | GGYRCC | 1 cut(s) | 572 |
| BspTNI | GGTCTC | 1 cut(s) | 227 |
| BsrI | ACTGG | 2 cut(s) | 310, 640 |
| BssECI | CCNNGG | 3 cut(s) | 72, 238, 628 |
| BssMI | GATC | 2 cut(s) | 171, 766 |
| BssT1I | CCWWGG | 2 cut(s) | 238, 628 |
| Bst2UI | CCWGG | 3 cut(s) | 37, 74, 533 |
| Bst4CI | ACNGT | 4 cut(s) | 80, 143, 448, 459 |
| Bst6I | CTCTTC | 2 cut(s) | 102, 583 |
| BstDEI | CTNAG | 6 cut(s) | 189, 321, 369, 407, 651, 714 |
| BstF5I | GGATG | 1 cut(s) | 620 |
| BstHHI | GCGC | 1 cut(s) | 513 |
| BstKTI | GATC | 2 cut(s) | 174, 769 |
| BstMAI | GTCTC | 2 cut(s) | 120, 227 |
| BstMBI | GATC | 2 cut(s) | 171, 766 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 470 |
| BstNI | CCWGG | 3 cut(s) | 37, 74, 533 |
| BstNSI | RCATGY | 1 cut(s) | 517 |
| BstSCI | CCNGG | 3 cut(s) | 35, 72, 531 |
| BstSFI | CTRYAG | 2 cut(s) | 455, 615 |
| BstV1I | GCAGC | 2 cut(s) | 324, 629 |
| BstV2I | GAAGAC | 1 cut(s) | 192 |
| BtsCI | GGATG | 1 cut(s) | 620 |
| BtsI | GCAGTG | 1 cut(s) | 97 |
| BtsIMutI | CAGTG | 5 cut(s) | 97, 148, 303, 453, 711 |
| CfoI | GCGC | 1 cut(s) | 513 |
| Cfr13I | GGNCC | 2 cut(s) | 185, 242 |
| Csp6I | GTAC | 2 cut(s) | 325, 573 |
| CviAII | CATG | 2 cut(s) | 492, 514 |
| CviJI | RGCY | 7 cut(s) | 248, 373, 391, 420, 427, 473, 730 |
| CviKI_1 | RGCY | 7 cut(s) | 248, 373, 391, 420, 427, 473, 730 |
| CviQI | GTAC | 2 cut(s) | 325, 573 |
| DdeI | CTNAG | 6 cut(s) | 189, 321, 369, 407, 651, 714 |
| DpnI | GATC | 2 cut(s) | 173, 768 |
| DpnII | GATC | 2 cut(s) | 171, 766 |
| DraI | TTTAAA | 1 cut(s) | 558 |
| DriI | GACNNNNNGTC | 1 cut(s) | 240 |
| Eam1104I | CTCTTC | 2 cut(s) | 102, 583 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 240 |
| EarI | CTCTTC | 2 cut(s) | 102, 583 |
| Eco130I | CCWWGG | 2 cut(s) | 238, 628 |
| Eco31I | GGTCTC | 1 cut(s) | 227 |
| Eco47I | GGWCC | 2 cut(s) | 185, 242 |
| EcoO109I | RGGNCCY | 1 cut(s) | 185 |
| EcoRI | GAATTC | 1 cut(s) | 5 |
| EcoRII | CCWGG | 3 cut(s) | 35, 72, 531 |
| EcoT14I | CCWWGG | 2 cut(s) | 238, 628 |
| ErhI | CCWWGG | 2 cut(s) | 238, 628 |
| FaeI | CATG | 2 cut(s) | 495, 517 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 537, 569 |
| FatI | CATG | 2 cut(s) | 491, 513 |
| FbaI | TGATCA | 1 cut(s) | 766 |
| Fnu4HI | GCNGC | 2 cut(s) | 313, 618 |
| FokI | GGATG | 1 cut(s) | 607 |
| Fsp4HI | GCNGC | 2 cut(s) | 313, 618 |
| FspBI | CTAG | 3 cut(s) | 362, 479, 689 |
| GlaI | GCGC | 1 cut(s) | 512 |
| GluI | GCNGC | 2 cut(s) | 313, 618 |
| HhaI | GCGC | 1 cut(s) | 513 |
| Hin1II | CATG | 2 cut(s) | 495, 517 |
| Hin6I | GCGC | 1 cut(s) | 511 |
| HinP1I | GCGC | 1 cut(s) | 511 |
| HincII | GTYRAC | 1 cut(s) | 42 |
| HindII | GTYRAC | 1 cut(s) | 42 |
| HinfI | GANTC | 4 cut(s) | 365, 482, 760, 809 |
| HphI | GGTGA | 1 cut(s) | 61 |
| Hpy166II | GTNNAC | 3 cut(s) | 42, 327, 451 |
| Hpy188I | TCNGA | 3 cut(s) | 192, 222, 370 |
| Hpy188III | TCNNGA | 6 cut(s) | 150, 362, 379, 538, 749, 764 |
| Hpy8I | GTNNAC | 3 cut(s) | 42, 327, 451 |
| HpyAV | CCTTC | 2 cut(s) | 111, 128 |
| HpyCH4III | ACNGT | 4 cut(s) | 80, 143, 448, 459 |
| HpyCH4IV | ACGT | 2 cut(s) | 197, 291 |
| HpyCH4V | TGCA | 6 cut(s) | 138, 386, 551, 617, 676, 711 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 470 |
| HpyF3I | CTNAG | 6 cut(s) | 189, 321, 369, 407, 651, 714 |
| HpySE526I | ACGT | 2 cut(s) | 197, 291 |
| Hsp92II | CATG | 2 cut(s) | 495, 517 |
| HspAI | GCGC | 1 cut(s) | 511 |
| KpnI | GGTACC | 1 cut(s) | 576 |
| Ksp22I | TGATCA | 1 cut(s) | 766 |
| Kzo9I | GATC | 2 cut(s) | 171, 766 |
| LguI | GCTCTTC | 1 cut(s) | 102 |
| LmnI | GCTCC | 1 cut(s) | 735 |
| Lsp1109I | GCAGC | 2 cut(s) | 324, 629 |
| LweI | GCATC | 2 cut(s) | 538, 629 |
| MaeI | CTAG | 3 cut(s) | 362, 479, 689 |
| MaeII | ACGT | 2 cut(s) | 197, 291 |
| MaeIII | GTNAC | 3 cut(s) | 437, 646, 691 |
| MalI | GATC | 2 cut(s) | 173, 768 |
| MboI | GATC | 2 cut(s) | 171, 766 |
| MboII | GAAGA | 3 cut(s) | 89, 192, 600 |
| MfeI | CAATTG | 2 cut(s) | 10, 608 |
| MhlI | GDGCHC | 1 cut(s) | 97 |
| MluCI | AATT | 9 cut(s) | 5, 10, 49, 112, 153, 330, 347, 594, 608 |
| MlyI | GAGTC | 2 cut(s) | 769, 803 |
| MnlI | CCTC | 5 cut(s) | 198, 330, 349, 501, 709 |
| MseI | TTAA | 5 cut(s) | 519, 557, 579, 723, 814 |
| MspR9I | CCNGG | 3 cut(s) | 37, 74, 533 |
| MunI | CAATTG | 2 cut(s) | 10, 608 |
| MvaI | CCWGG | 3 cut(s) | 37, 74, 533 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 470 |
| NdeII | GATC | 2 cut(s) | 171, 766 |
| NlaIII | CATG | 2 cut(s) | 495, 517 |
| NlaIV | GGNNCC | 3 cut(s) | 186, 574, 731 |
| NmuCI | GTSAC | 1 cut(s) | 691 |
| NspI | RCATGY | 1 cut(s) | 517 |
| PciSI | GCTCTTC | 1 cut(s) | 102 |
| PfeI | GAWTC | 2 cut(s) | 365, 482 |
| PkrI | GCNGC | 2 cut(s) | 314, 619 |
| PleI | GAGTC | 2 cut(s) | 768, 803 |
| PpsI | GAGTC | 2 cut(s) | 768, 803 |
| PpuMI | RGGWCCY | 1 cut(s) | 185 |
| PsiI | TTATAA | 1 cut(s) | 162 |
| Psp1406I | AACGTT | 1 cut(s) | 291 |
| Psp5II | RGGWCCY | 1 cut(s) | 185 |
| Psp6I | CCWGG | 3 cut(s) | 35, 72, 531 |
| PspGI | CCWGG | 3 cut(s) | 35, 72, 531 |
| PspN4I | GGNNCC | 3 cut(s) | 186, 574, 731 |
| PspPI | GGNCC | 2 cut(s) | 185, 242 |
| PspPPI | RGGWCCY | 1 cut(s) | 185 |
| PstI | CTGCAG | 1 cut(s) | 619 |
| RsaI | GTAC | 2 cut(s) | 326, 574 |
| RsaNI | GTAC | 2 cut(s) | 325, 573 |
| SapI | GCTCTTC | 1 cut(s) | 102 |
| SaqAI | TTAA | 5 cut(s) | 519, 557, 579, 723, 814 |
| SatI | GCNGC | 2 cut(s) | 313, 618 |
| Sau3AI | GATC | 2 cut(s) | 171, 766 |
| Sau96I | GGNCC | 2 cut(s) | 185, 242 |
| SchI | GAGTC | 2 cut(s) | 769, 803 |
| ScrFI | CCNGG | 3 cut(s) | 37, 74, 533 |
| SduI | GDGCHC | 1 cut(s) | 97 |
| SfaNI | GCATC | 2 cut(s) | 538, 629 |
| SfcI | CTRYAG | 2 cut(s) | 455, 615 |
| SinI | GGWCC | 2 cut(s) | 185, 242 |
| SmlI | CTYRAG | 1 cut(s) | 428 |
| SmoI | CTYRAG | 1 cut(s) | 428 |
| Sse9I | AATT | 9 cut(s) | 5, 10, 49, 112, 153, 330, 347, 594, 608 |
| SspI | AATATT | 1 cut(s) | 523 |
| SspMI | CTAG | 3 cut(s) | 362, 479, 689 |
| StyD4I | CCNGG | 3 cut(s) | 35, 72, 531 |
| StyI | CCWWGG | 2 cut(s) | 238, 628 |
| TaaI | ACNGT | 4 cut(s) | 80, 143, 448, 459 |
| TaiI | ACGT | 2 cut(s) | 200, 294 |
| TasI | AATT | 9 cut(s) | 5, 10, 49, 112, 153, 330, 347, 594, 608 |
| TatI | WGTACW | 1 cut(s) | 324 |
| TfiI | GAWTC | 2 cut(s) | 365, 482 |
| Tru1I | TTAA | 5 cut(s) | 519, 557, 579, 723, 814 |
| Tru9I | TTAA | 5 cut(s) | 519, 557, 579, 723, 814 |
| TscAI | CASTG | 5 cut(s) | 97, 148, 310, 453, 718 |
| TseFI | GTSAC | 1 cut(s) | 691 |
| TseI | GCWGC | 2 cut(s) | 312, 617 |
| Tsp45I | GTSAC | 1 cut(s) | 691 |
| TspDTI | ATGAA | 1 cut(s) | 390 |
| TspGWI | ACGGA | 1 cut(s) | 239 |
| TspRI | CASTG | 5 cut(s) | 97, 148, 310, 453, 718 |
| VpaK11BI | GGWCC | 2 cut(s) | 185, 242 |
| XapI | RAATTY | 2 cut(s) | 5, 153 |
| XbaI | TCTAGA | 1 cut(s) | 361 |
| XceI | RCATGY | 1 cut(s) | 517 |
| XspI | CTAG | 3 cut(s) | 362, 479, 689 |