Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 153, 271 |
| AclWI | GGATC | 3 cut(s) | 401, 446, 459 |
| AcsI | RAATTY | 3 cut(s) | 79, 185, 347 |
| AfaI | GTAC | 2 cut(s) | 364, 436 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 94 |
| AgsI | TTSAA | 4 cut(s) | 161, 190, 302, 321 |
| AhlI | ACTAGT | 1 cut(s) | 163 |
| AjnI | CCWGG | 1 cut(s) | 454 |
| AluBI | AGCT | 3 cut(s) | 93, 414, 463 |
| AluI | AGCT | 3 cut(s) | 93, 414, 463 |
| Alw21I | GWGCWC | 1 cut(s) | 236 |
| Alw44I | GTGCAC | 1 cut(s) | 232 |
| AlwI | GGATC | 3 cut(s) | 401, 446, 459 |
| AoxI | GGCC | 1 cut(s) | 74 |
| ApaLI | GTGCAC | 1 cut(s) | 232 |
| ApeKI | GCWGC | 1 cut(s) | 460 |
| ApoI | RAATTY | 3 cut(s) | 79, 185, 347 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 332, 364 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 286 |
| AsuHPI | GGTGA | 3 cut(s) | 237, 361, 417 |
| BaeGI | GKGCMC | 1 cut(s) | 236 |
| BamHI | GGATCC | 1 cut(s) | 451 |
| BbsI | GAAGAC | 1 cut(s) | 29 |
| Bbv12I | GWGCWC | 1 cut(s) | 236 |
| BbvI | GCAGC | 1 cut(s) | 472 |
| BccI | CCATC | 1 cut(s) | 92 |
| BciT130I | CCWGG | 1 cut(s) | 456 |
| BcuI | ACTAGT | 1 cut(s) | 163 |
| BfaI | CTAG | 5 cut(s) | 56, 164, 174, 327, 377 |
| BisI | GCNGC | 1 cut(s) | 461 |
| BlsI | GCNGC | 1 cut(s) | 462 |
| BmcAI | AGTACT | 1 cut(s) | 364 |
| Bme1390I | CCNGG | 1 cut(s) | 456 |
| BmiI | GGNNCC | 1 cut(s) | 453 |
| BmrFI | CCNGG | 1 cut(s) | 456 |
| BmrI | ACTGGG | 1 cut(s) | 352 |
| BmsI | GCATC | 1 cut(s) | 472 |
| BmuI | ACTGGG | 1 cut(s) | 352 |
| BpiI | GAAGAC | 1 cut(s) | 29 |
| BsaJI | CCNNGG | 2 cut(s) | 455, 477 |
| BsaWI | WCCGGW | 1 cut(s) | 87 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 94 |
| Bse118I | RCCGGY | 1 cut(s) | 427 |
| Bse1I | ACTGG | 3 cut(s) | 248, 347, 372 |
| BseBI | CCWGG | 1 cut(s) | 456 |
| BseDI | CCNNGG | 2 cut(s) | 455, 477 |
| BseGI | GGATG | 2 cut(s) | 103, 487 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 94 |
| BseNI | ACTGG | 3 cut(s) | 248, 347, 372 |
| BseSI | GKGCMC | 1 cut(s) | 236 |
| BseXI | GCAGC | 1 cut(s) | 472 |
| Bsh1236I | CGCG | 2 cut(s) | 153, 271 |
| BshFI | GGCC | 1 cut(s) | 76 |
| BsiHKAI | GWGCWC | 1 cut(s) | 236 |
| BsiSI | CCGG | 2 cut(s) | 88, 428 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 94 |
| BsmI | GAATGC | 1 cut(s) | 288 |
| BsnI | GGCC | 1 cut(s) | 76 |
| Bsp1286I | GDGCHC | 1 cut(s) | 236 |
| Bsp143I | GATC | 2 cut(s) | 406, 451 |
| BspANI | GGCC | 1 cut(s) | 76 |
| BspFNI | CGCG | 2 cut(s) | 153, 271 |
| BspLI | GGNNCC | 1 cut(s) | 453 |
| BspPI | GGATC | 3 cut(s) | 401, 446, 459 |
| BsrFI | RCCGGY | 1 cut(s) | 427 |
| BsrI | ACTGG | 3 cut(s) | 248, 347, 372 |
| BssAI | RCCGGY | 1 cut(s) | 427 |
| BssECI | CCNNGG | 2 cut(s) | 455, 477 |
| BssMI | GATC | 2 cut(s) | 406, 451 |
| Bst2UI | CCWGG | 1 cut(s) | 456 |
| Bst4CI | ACNGT | 1 cut(s) | 313 |
| Bst6I | CTCTTC | 1 cut(s) | 192 |
| BstDSI | CCRYGG | 1 cut(s) | 477 |
| BstF5I | GGATG | 2 cut(s) | 103, 487 |
| BstFNI | CGCG | 2 cut(s) | 153, 271 |
| BstKTI | GATC | 2 cut(s) | 409, 454 |
| BstMBI | GATC | 2 cut(s) | 406, 451 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 99, 482 |
| BstNI | CCWGG | 1 cut(s) | 456 |
| BstSCI | CCNGG | 1 cut(s) | 454 |
| BstSLI | GKGCMC | 1 cut(s) | 236 |
| BstUI | CGCG | 2 cut(s) | 153, 271 |
| BstV1I | GCAGC | 1 cut(s) | 472 |
| BstV2I | GAAGAC | 1 cut(s) | 29 |
| BstX2I | RGATCY | 1 cut(s) | 451 |
| BstYI | RGATCY | 1 cut(s) | 451 |
| BsuRI | GGCC | 1 cut(s) | 76 |
| BtgI | CCRYGG | 1 cut(s) | 477 |
| BtsCI | GGATG | 2 cut(s) | 103, 487 |
| BtsIMutI | CAGTG | 3 cut(s) | 73, 255, 381 |
| Cfr10I | RCCGGY | 1 cut(s) | 427 |
| Csp6I | GTAC | 2 cut(s) | 363, 435 |
| CviAII | CATG | 1 cut(s) | 140 |
| CviJI | RGCY | 7 cut(s) | 76, 93, 128, 325, 380, 414, 463 |
| CviKI_1 | RGCY | 7 cut(s) | 76, 93, 128, 325, 380, 414, 463 |
| CviQI | GTAC | 2 cut(s) | 363, 435 |
| DpnI | GATC | 2 cut(s) | 408, 453 |
| DpnII | GATC | 2 cut(s) | 406, 451 |
| Eam1104I | CTCTTC | 1 cut(s) | 192 |
| EarI | CTCTTC | 1 cut(s) | 192 |
| EcoRI | GAATTC | 1 cut(s) | 185 |
| EcoRII | CCWGG | 1 cut(s) | 454 |
| FaeI | CATG | 1 cut(s) | 143 |
| FaiI | YATR | 3 cut(s) | 141, 389, 495 |
| FatI | CATG | 1 cut(s) | 139 |
| Fnu4HI | GCNGC | 1 cut(s) | 461 |
| FokI | GGATG | 2 cut(s) | 110, 494 |
| Fsp4HI | GCNGC | 1 cut(s) | 461 |
| FspBI | CTAG | 5 cut(s) | 56, 164, 174, 327, 377 |
| GluI | GCNGC | 1 cut(s) | 461 |
| HaeIII | GGCC | 1 cut(s) | 76 |
| HapII | CCGG | 2 cut(s) | 88, 428 |
| Hin1II | CATG | 1 cut(s) | 143 |
| HinfI | GANTC | 1 cut(s) | 170 |
| HpaII | CCGG | 2 cut(s) | 88, 428 |
| HphI | GGTGA | 3 cut(s) | 237, 361, 417 |
| Hpy166II | GTNNAC | 3 cut(s) | 8, 169, 234 |
| Hpy188I | TCNGA | 3 cut(s) | 85, 184, 411 |
| Hpy188III | TCNNGA | 3 cut(s) | 56, 119, 291 |
| Hpy8I | GTNNAC | 3 cut(s) | 8, 169, 234 |
| HpyCH4III | ACNGT | 1 cut(s) | 313 |
| HpyCH4V | TGCA | 3 cut(s) | 139, 234, 253 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 99, 482 |
| Hsp92II | CATG | 1 cut(s) | 143 |
| Kzo9I | GATC | 2 cut(s) | 406, 451 |
| LmnI | GCTCC | 1 cut(s) | 90 |
| LpnPI | CCDG | 8 cut(s) | 101, 261, 276, 328, 385, 441, 441, 468 |
| Lsp1109I | GCAGC | 1 cut(s) | 472 |
| LweI | GCATC | 1 cut(s) | 472 |
| MaeI | CTAG | 5 cut(s) | 56, 164, 174, 327, 377 |
| MaeIII | GTNAC | 2 cut(s) | 313, 329 |
| MalI | GATC | 2 cut(s) | 408, 453 |
| MboI | GATC | 2 cut(s) | 406, 451 |
| MboII | GAAGA | 3 cut(s) | 34, 107, 209 |
| MflI | RGATCY | 1 cut(s) | 451 |
| MhlI | GDGCHC | 1 cut(s) | 236 |
| MluCI | AATT | 4 cut(s) | 60, 79, 185, 347 |
| MlyI | GAGTC | 1 cut(s) | 164 |
| MnlI | CCTC | 2 cut(s) | 187, 193 |
| MroXI | GAANNNNTTC | 1 cut(s) | 286 |
| MseI | TTAA | 1 cut(s) | 526 |
| MslI | CAYNNNNRTG | 2 cut(s) | 138, 386 |
| MspI | CCGG | 2 cut(s) | 88, 428 |
| MspR9I | CCNGG | 1 cut(s) | 456 |
| Mva1269I | GAATGC | 1 cut(s) | 288 |
| MvaI | CCWGG | 1 cut(s) | 456 |
| MvnI | CGCG | 2 cut(s) | 153, 271 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 99, 482 |
| NdeII | GATC | 2 cut(s) | 406, 451 |
| NlaIII | CATG | 1 cut(s) | 143 |
| NlaIV | GGNNCC | 1 cut(s) | 453 |
| PctI | GAATGC | 1 cut(s) | 288 |
| PdmI | GAANNNNTTC | 1 cut(s) | 286 |
| PkrI | GCNGC | 1 cut(s) | 462 |
| PleI | GAGTC | 1 cut(s) | 164 |
| PpsI | GAGTC | 1 cut(s) | 164 |
| Psp6I | CCWGG | 1 cut(s) | 454 |
| PspGI | CCWGG | 1 cut(s) | 454 |
| PspN4I | GGNNCC | 1 cut(s) | 453 |
| PsuI | RGATCY | 1 cut(s) | 451 |
| RsaI | GTAC | 2 cut(s) | 364, 436 |
| RsaNI | GTAC | 2 cut(s) | 363, 435 |
| RseI | CAYNNNNRTG | 2 cut(s) | 138, 386 |
| SaqAI | TTAA | 1 cut(s) | 526 |
| SatI | GCNGC | 1 cut(s) | 461 |
| Sau3AI | GATC | 2 cut(s) | 406, 451 |
| ScaI | AGTACT | 1 cut(s) | 364 |
| SchI | GAGTC | 1 cut(s) | 164 |
| ScrFI | CCNGG | 1 cut(s) | 456 |
| SduI | GDGCHC | 1 cut(s) | 236 |
| SetI | ASST | 4 cut(s) | 95, 179, 416, 465 |
| SfaNI | GCATC | 1 cut(s) | 472 |
| SgrAI | CRCCGGYG | 1 cut(s) | 427 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 138, 386 |
| SpeI | ACTAGT | 1 cut(s) | 163 |
| Sse9I | AATT | 4 cut(s) | 60, 79, 185, 347 |
| SspMI | CTAG | 5 cut(s) | 56, 164, 174, 327, 377 |
| StyD4I | CCNGG | 1 cut(s) | 454 |
| TaaI | ACNGT | 1 cut(s) | 313 |
| TaqI | TCGA | 1 cut(s) | 120 |
| TasI | AATT | 4 cut(s) | 60, 79, 185, 347 |
| TatI | WGTACW | 2 cut(s) | 362, 434 |
| Tru1I | TTAA | 1 cut(s) | 526 |
| Tru9I | TTAA | 1 cut(s) | 526 |
| TscAI | CASTG | 3 cut(s) | 73, 255, 388 |
| TseI | GCWGC | 1 cut(s) | 460 |
| TspDTI | ATGAA | 2 cut(s) | 156, 482 |
| TspGWI | ACGGA | 1 cut(s) | 494 |
| TspRI | CASTG | 3 cut(s) | 73, 255, 388 |
| VneI | GTGCAC | 1 cut(s) | 232 |
| XapI | RAATTY | 3 cut(s) | 79, 185, 347 |
| XbaI | TCTAGA | 1 cut(s) | 55 |
| XmnI | GAANNNNTTC | 1 cut(s) | 286 |
| XspI | CTAG | 5 cut(s) | 56, 164, 174, 327, 377 |
| ZrmI | AGTACT | 1 cut(s) | 364 |