Belongs to the protein kinase superfamily
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 410 |
| AccB1I | GGYRCC | 1 cut(s) | 410 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 497 |
| AciI | CCGC | 1 cut(s) | 635 |
| AclWI | GGATC | 1 cut(s) | 582 |
| AfaI | GTAC | 3 cut(s) | 355, 412, 877 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 497, 966 |
| AgsI | TTSAA | 5 cut(s) | 288, 386, 448, 796, 839 |
| AjnI | CCWGG | 1 cut(s) | 758 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 939, 971 |
| AluBI | AGCT | 9 cut(s) | 14, 74, 256, 392, 527, 599, 670, 777, 854 |
| AluI | AGCT | 9 cut(s) | 14, 74, 256, 392, 527, 599, 670, 777, 854 |
| Alw26I | GTCTC | 3 cut(s) | 9, 85, 319 |
| AlwI | GGATC | 1 cut(s) | 582 |
| AoxI | GGCC | 1 cut(s) | 459 |
| ApaI | GGGCCC | 1 cut(s) | 463 |
| ApeKI | GCWGC | 1 cut(s) | 389 |
| AseI | ATTAAT | 1 cut(s) | 513 |
| Asp718I | GGTACC | 1 cut(s) | 410 |
| AspS9I | GGNCC | 4 cut(s) | 459, 460, 754, 943 |
| AsuHPI | GGTGA | 1 cut(s) | 655 |
| AsuNHI | GCTAGC | 1 cut(s) | 10 |
| AvaII | GGWCC | 2 cut(s) | 754, 943 |
| BaeGI | GKGCMC | 2 cut(s) | 463, 484 |
| BanI | GGYRCC | 1 cut(s) | 410 |
| BanII | GRGCYC | 1 cut(s) | 463 |
| BbvI | GCAGC | 1 cut(s) | 401 |
| BccI | CCATC | 5 cut(s) | 434, 492, 515, 640, 922 |
| BciT130I | CCWGG | 1 cut(s) | 760 |
| BclI | TGATCA | 1 cut(s) | 121 |
| BcoDI | GTCTC | 3 cut(s) | 9, 85, 319 |
| BfaI | CTAG | 4 cut(s) | 11, 393, 624, 704 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 458 |
| BglII | AGATCT | 1 cut(s) | 433 |
| BisI | GCNGC | 2 cut(s) | 390, 636 |
| BlsI | GCNGC | 2 cut(s) | 391, 637 |
| Bme1390I | CCNGG | 1 cut(s) | 760 |
| Bme18I | GGWCC | 2 cut(s) | 754, 943 |
| BmgT120I | GGNCC | 4 cut(s) | 459, 460, 754, 943 |
| BmiI | GGNNCC | 5 cut(s) | 412, 460, 461, 756, 944 |
| BmrFI | CCNGG | 1 cut(s) | 760 |
| BmsI | GCATC | 2 cut(s) | 847, 895 |
| BmtI | GCTAGC | 1 cut(s) | 14 |
| BpuEI | CTTGAG | 1 cut(s) | 635 |
| Bsa29I | ATCGAT | 1 cut(s) | 105 |
| BsaAI | YACGTR | 1 cut(s) | 697 |
| BsaI | GGTCTC | 1 cut(s) | 85 |
| BsaJI | CCNNGG | 1 cut(s) | 758 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 868, 898 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 497, 966 |
| Bse3DI | GCAATG | 2 cut(s) | 463, 630 |
| BseBI | CCWGG | 1 cut(s) | 760 |
| BseCI | ATCGAT | 1 cut(s) | 105 |
| BseDI | CCNNGG | 1 cut(s) | 758 |
| BseGI | GGATG | 3 cut(s) | 426, 484, 910 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 497, 966 |
| BseMI | GCAATG | 2 cut(s) | 463, 630 |
| BseSI | GKGCMC | 2 cut(s) | 463, 484 |
| BseXI | GCAGC | 1 cut(s) | 401 |
| BshFI | GGCC | 1 cut(s) | 461 |
| BshNI | GGYRCC | 1 cut(s) | 410 |
| BshVI | ATCGAT | 1 cut(s) | 105 |
| BslFI | GGGAC | 3 cut(s) | 597, 740, 956 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 497, 966 |
| BsmAI | GTCTC | 3 cut(s) | 9, 85, 319 |
| BsmFI | GGGAC | 3 cut(s) | 597, 740, 956 |
| BsnI | GGCC | 1 cut(s) | 461 |
| Bso31I | GGTCTC | 1 cut(s) | 85 |
| Bsp120I | GGGCCC | 1 cut(s) | 459 |
| Bsp1286I | GDGCHC | 2 cut(s) | 463, 484 |
| Bsp143I | GATC | 4 cut(s) | 91, 121, 433, 574 |
| BspACI | CCGC | 1 cut(s) | 635 |
| BspANI | GGCC | 1 cut(s) | 461 |
| BspDI | ATCGAT | 1 cut(s) | 105 |
| BspLI | GGNNCC | 5 cut(s) | 412, 460, 461, 756, 944 |
| BspOI | GCTAGC | 1 cut(s) | 14 |
| BspPI | GGATC | 1 cut(s) | 582 |
| BspT107I | GGYRCC | 1 cut(s) | 410 |
| BspTNI | GGTCTC | 1 cut(s) | 85 |
| BsrDI | GCAATG | 2 cut(s) | 463, 630 |
| BssECI | CCNNGG | 1 cut(s) | 758 |
| BssMI | GATC | 4 cut(s) | 91, 121, 433, 574 |
| Bst2UI | CCWGG | 1 cut(s) | 760 |
| Bst6I | CTCTTC | 1 cut(s) | 721 |
| BstBAI | YACGTR | 1 cut(s) | 697 |
| BstC8I | GCNNGC | 3 cut(s) | 12, 672, 779 |
| BstDEI | CTNAG | 1 cut(s) | 593 |
| BstF5I | GGATG | 3 cut(s) | 426, 484, 910 |
| BstKTI | GATC | 4 cut(s) | 94, 124, 436, 577 |
| BstMAI | GTCTC | 3 cut(s) | 9, 85, 319 |
| BstMBI | GATC | 4 cut(s) | 91, 121, 433, 574 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 458, 700 |
| BstNI | CCWGG | 1 cut(s) | 760 |
| BstSCI | CCNGG | 1 cut(s) | 758 |
| BstSLI | GKGCMC | 2 cut(s) | 463, 484 |
| BstV1I | GCAGC | 1 cut(s) | 401 |
| BstX2I | RGATCY | 1 cut(s) | 433 |
| BstYI | RGATCY | 1 cut(s) | 433 |
| Bsu15I | ATCGAT | 1 cut(s) | 105 |
| BsuRI | GGCC | 1 cut(s) | 461 |
| BsuTUI | ATCGAT | 1 cut(s) | 105 |
| BtsCI | GGATG | 3 cut(s) | 426, 484, 910 |
| Cac8I | GCNNGC | 3 cut(s) | 12, 672, 779 |
| Cfr13I | GGNCC | 4 cut(s) | 459, 460, 754, 943 |
| ClaI | ATCGAT | 1 cut(s) | 105 |
| CseI | GACGC | 1 cut(s) | 464 |
| Csp6I | GTAC | 3 cut(s) | 354, 411, 876 |
| CviAII | CATG | 3 cut(s) | 329, 415, 675 |
| CviQI | GTAC | 3 cut(s) | 354, 411, 876 |
| DdeI | CTNAG | 1 cut(s) | 593 |
| DpnI | GATC | 4 cut(s) | 93, 123, 435, 576 |
| DpnII | GATC | 4 cut(s) | 91, 121, 433, 574 |
| Eam1104I | CTCTTC | 1 cut(s) | 721 |
| EarI | CTCTTC | 1 cut(s) | 721 |
| Eco24I | GRGCYC | 1 cut(s) | 463 |
| Eco31I | GGTCTC | 1 cut(s) | 85 |
| Eco47I | GGWCC | 2 cut(s) | 754, 943 |
| EcoO109I | RGGNCCY | 1 cut(s) | 459 |
| EcoRII | CCWGG | 1 cut(s) | 758 |
| EcoT22I | ATGCAT | 1 cut(s) | 420 |
| EcoT38I | GRGCYC | 1 cut(s) | 463 |
| FaeI | CATG | 3 cut(s) | 332, 418, 678 |
| FaqI | GGGAC | 3 cut(s) | 597, 740, 956 |
| FatI | CATG | 3 cut(s) | 328, 414, 674 |
| FauNDI | CATATG | 1 cut(s) | 579 |
| FbaI | TGATCA | 1 cut(s) | 121 |
| Fnu4HI | GCNGC | 2 cut(s) | 390, 636 |
| FokI | GGATG | 3 cut(s) | 413, 471, 917 |
| FriOI | GRGCYC | 1 cut(s) | 463 |
| Fsp4HI | GCNGC | 2 cut(s) | 390, 636 |
| FspBI | CTAG | 4 cut(s) | 11, 393, 624, 704 |
| GluI | GCNGC | 2 cut(s) | 390, 636 |
| HaeIII | GGCC | 1 cut(s) | 461 |
| HgaI | GACGC | 1 cut(s) | 464 |
| Hin1II | CATG | 3 cut(s) | 332, 418, 678 |
| HinfI | GANTC | 5 cut(s) | 107, 113, 163, 619, 713 |
| HphI | GGTGA | 1 cut(s) | 655 |
| Hpy166II | GTNNAC | 3 cut(s) | 221, 251, 712 |
| Hpy188I | TCNGA | 4 cut(s) | 46, 112, 520, 939 |
| Hpy188III | TCNNGA | 6 cut(s) | 119, 614, 683, 728, 796, 836 |
| Hpy8I | GTNNAC | 3 cut(s) | 221, 251, 712 |
| Hpy99I | CGWCG | 1 cut(s) | 480 |
| HpyAV | CCTTC | 4 cut(s) | 13, 57, 331, 833 |
| HpyCH4IV | ACGT | 2 cut(s) | 586, 696 |
| HpyCH4V | TGCA | 3 cut(s) | 155, 418, 468 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 458, 700 |
| HpyF3I | CTNAG | 1 cut(s) | 593 |
| HpySE526I | ACGT | 2 cut(s) | 586, 696 |
| Hsp92II | CATG | 3 cut(s) | 332, 418, 678 |
| KpnI | GGTACC | 1 cut(s) | 414 |
| Ksp22I | TGATCA | 1 cut(s) | 121 |
| Kzo9I | GATC | 4 cut(s) | 91, 121, 433, 574 |
| LmnI | GCTCC | 3 cut(s) | 524, 889, 913 |
| LpnPI | CCDG | 6 cut(s) | 393, 656, 713, 745, 763, 772 |
| Lsp1109I | GCAGC | 1 cut(s) | 401 |
| LweI | GCATC | 2 cut(s) | 847, 895 |
| MaeI | CTAG | 4 cut(s) | 11, 393, 624, 704 |
| MaeII | ACGT | 2 cut(s) | 586, 696 |
| MaeIII | GTNAC | 1 cut(s) | 969 |
| MalI | GATC | 4 cut(s) | 93, 123, 435, 576 |
| MboI | GATC | 4 cut(s) | 91, 121, 433, 574 |
| MboII | GAAGA | 1 cut(s) | 708 |
| MfeI | CAATTG | 3 cut(s) | 663, 784, 947 |
| MflI | RGATCY | 1 cut(s) | 433 |
| MhlI | GDGCHC | 2 cut(s) | 463, 484 |
| MlyI | GAGTC | 2 cut(s) | 172, 707 |
| MnlI | CCTC | 9 cut(s) | 23, 208, 210, 214, 325, 535, 545, 634, 933 |
| Mph1103I | ATGCAT | 1 cut(s) | 420 |
| MseI | TTAA | 8 cut(s) | 26, 135, 258, 272, 507, 513, 657, 738 |
| MspR9I | CCNGG | 1 cut(s) | 760 |
| MunI | CAATTG | 3 cut(s) | 663, 784, 947 |
| MvaI | CCWGG | 1 cut(s) | 760 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 458, 700 |
| NdeI | CATATG | 1 cut(s) | 579 |
| NdeII | GATC | 4 cut(s) | 91, 121, 433, 574 |
| NheI | GCTAGC | 1 cut(s) | 10 |
| NlaIII | CATG | 3 cut(s) | 332, 418, 678 |
| NlaIV | GGNNCC | 5 cut(s) | 412, 460, 461, 756, 944 |
| NsiI | ATGCAT | 1 cut(s) | 420 |
| PfeI | GAWTC | 3 cut(s) | 107, 113, 619 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 497 |
| PkrI | GCNGC | 2 cut(s) | 391, 637 |
| PleI | GAGTC | 2 cut(s) | 171, 707 |
| PpsI | GAGTC | 2 cut(s) | 171, 707 |
| Ppu21I | YACGTR | 1 cut(s) | 697 |
| PshBI | ATTAAT | 1 cut(s) | 513 |
| Psp6I | CCWGG | 1 cut(s) | 758 |
| PspGI | CCWGG | 1 cut(s) | 758 |
| PspN4I | GGNNCC | 5 cut(s) | 412, 460, 461, 756, 944 |
| PspOMI | GGGCCC | 1 cut(s) | 459 |
| PspPI | GGNCC | 4 cut(s) | 459, 460, 754, 943 |
| PsuI | RGATCY | 1 cut(s) | 433 |
| RsaI | GTAC | 3 cut(s) | 355, 412, 877 |
| RsaNI | GTAC | 3 cut(s) | 354, 411, 876 |
| SaqAI | TTAA | 8 cut(s) | 26, 135, 258, 272, 507, 513, 657, 738 |
| SatI | GCNGC | 2 cut(s) | 390, 636 |
| Sau3AI | GATC | 4 cut(s) | 91, 121, 433, 574 |
| Sau96I | GGNCC | 4 cut(s) | 459, 460, 754, 943 |
| SchI | GAGTC | 2 cut(s) | 172, 707 |
| ScrFI | CCNGG | 1 cut(s) | 760 |
| SduI | GDGCHC | 2 cut(s) | 463, 484 |
| SfaNI | GCATC | 2 cut(s) | 847, 895 |
| SinI | GGWCC | 2 cut(s) | 754, 943 |
| SmlI | CTYRAG | 1 cut(s) | 614 |
| SmoI | CTYRAG | 1 cut(s) | 614 |
| SsiI | CCGC | 1 cut(s) | 635 |
| SspI | AATATT | 1 cut(s) | 291 |
| SspMI | CTAG | 4 cut(s) | 11, 393, 624, 704 |
| StyD4I | CCNGG | 1 cut(s) | 758 |
| TaiI | ACGT | 2 cut(s) | 589, 699 |
| TaqI | TCGA | 1 cut(s) | 105 |
| TauI | GCSGC | 1 cut(s) | 638 |
| TfiI | GAWTC | 3 cut(s) | 107, 113, 619 |
| Tru1I | TTAA | 8 cut(s) | 26, 135, 258, 272, 507, 513, 657, 738 |
| Tru9I | TTAA | 8 cut(s) | 26, 135, 258, 272, 507, 513, 657, 738 |
| TseI | GCWGC | 1 cut(s) | 389 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 497 |
| VpaK11BI | GGWCC | 2 cut(s) | 754, 943 |
| VspI | ATTAAT | 1 cut(s) | 513 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 498 |
| XspI | CTAG | 4 cut(s) | 11, 393, 624, 704 |
| Zsp2I | ATGCAT | 1 cut(s) | 420 |