Rroxscaffold_1G00073920

Di-glucose binding within endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000088
Physical Location & Seq
Forward (+)
94847450 .. 94854739
7290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00073920.1

Sequence Viewer

Length: 1980 bp
ATGGCTCCAATTAGGAGGTGCCCTTCTGGGGCTAAAATCCAGCTTCAAGCTGGGTCTCTACTCTTCTTGTTCGGCCTCTGCTTCCAGGGTTACACTTGGTTTCAAGTTTTCACGGCTAAAAATGCTACTACTGACCCATCTGAAGTTCGAGCGTTGAACTCAATATTCTCACAATGGGATACTCAAGCGGTGGCGCTGTGGAATATCAGTGGGGAGCCATGCAGTGGATCAGCCATCAATGGAACTGAATTTGAGAGCCCTCTTAATAACCCGGCCATCATTTGCGACTGTACTTATGACAGTGGCACCACCTGCCATATTACCCAACTGAGAGTCTATGCTCTAAACAAACGAGGAGTGTTTGCAAAGGAACTTTTGGCTTTGAGATACCTGACCTTCTTGAAAATCGATCAGAATTATTTCACAGGTCCTCTACCAGCATGGATCGGCAATATGACTGCATTGACATTATTGTCAATTGCTCACAATGCATTCTCTGGGCCCATTCCGAAGGAGCTTGGAAACCTCAAGGACTTAAATATGCTGTCCTTTGGAATAAATAACTTCTCCGGAACACTCCCTCCCGAACTTGGTAATTTGGTCAAGCTTCAACAACTTTATATAAACAGCTGCGGACTCGGTGGTGAAATTCCTTCAACATTTGCTAACCTCCGCAATATGCAAGTCCTATGGGCATCAGACAATCCCTTCTCAGGAAGGATACCTGATTTCATTGGGAATTGGACAAAGATAAACTCTCTGCGATTTCAAGGGAACTCTTTTAGAGGTCCAATACCGACCAGTTTTTCTCAACTAACTGCCTTGAATTCTCTGCGAATCAGTGATATCTACAATGTAAGCTCCTCTCTTGATTTCATTAAGAATTTGAAGAACTTGACTGATTTAGCGCTACGAAATGCATTAATTACTGGTAGCATCCCTTCTGATATTGGAGAATACCAAAGTTTACAGATACTGGATTTGAGTTTCAACAATTTAACAGGTCAACTTCCAGGTGGCTTGTTCAACATGAGTTCTCTAACATACTTATTTCTTGGAAACAATAGCTTGCAGGGAGCTCTTCCTAGCCAAAAGAGTGATATTCTTCACACTATAGATTTGTCTTACAACTATTTATCAGGAAGTCTTCCCCCATGGGTAACCTCAATGTCACAGCTGAACTTAGTGGTGAACAACTTCACATTGGACAGTTCAAACATAAATCTTCCTGGATTAAATTGCCTTCAAAGAAATTTTCCATGCAATAGAAATACCCCAAAATATGCAAAATTCTCAATCAACTGTGGTGGACTAGAATTGAGAGGGAGTGATGACATATTGTATGAGACTGAAGACTCAGCTCTTGGCCCAGCAACGTTTATTGTAACAAGTTCAGAGAAATGGGCTGTTAGCAATGTGGGTTTGTTTGCTGACAGAAAGAATCCGAGCTTTGTGGTAAATACCCTGGCACAAGTCAATAGGACAGATGTGACCCCAGAGCTTTTCCAGACCTCAAGGTTGTCTCCAGGATCACTGAGATACTATGGCCTGGGTCTTGAGAATGGGCCTTATACTGTAAAATTGCAATTTGCAGAAACAGTTTTCGATGATCGTGCCATGCAAACTTGGCAAAGTCTAGGACGGCGTATGTTTGATATCTATATTCAGGGGAACCTTGTATTGAAGGACTTTGACATATCAAAGGAGGCAGGAGGGGTTTTAAGAGCTGTTGTGAAAACCTTCAGTGTTAATGTTTCAGAGAATTATCTTGAAATTCATTTATTCTGGGCTGGTAAGGGGACCTGCTGCACACCTGATCAAGGTCATTACGGCCCACTAATAGCTGCTGTGCATGCTGCTTCAGATTTTACACCAACTGTTTCTGGTGTTCCACCAACTACTGTAGGAATGAAGAGCAGGACAGGACTGATGGGTATTCTGGTTCCTGTTGGAGTTTTGGTGTTGCTAGTTATAATTTGA

Protein Analysis

659

Amino Acids

72.1

Weight (kDa)

6.61

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 191 - 280 1.4e-07 Leucine-rich repeat region
LRR_14 PF23598 269 - 355 4.3e-07 Leucine-rich repeat region
Malectin PF11721 431 - 617 1.7e-37 Malectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1973
AarI CACCTGC 1 cut(s) 320
AasI GACNNNNNNGTC 1 cut(s) 472
Acc36I ACCTGC 2 cut(s) 320, 1811
AccB1I GGYRCC 2 cut(s) 18, 305
AccB7I CCANNNNNTGG 1 cut(s) 224
AccIII TCCGGA 1 cut(s) 569
AciI CCGC 3 cut(s) 188, 633, 673
AclI AACGTT 1 cut(s) 1376
AclWI GGATC 3 cut(s) 235, 452, 1537
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 7 cut(s) 248, 648, 826, 883, 1252, 1289, 1773
AcuI CTGAAG 4 cut(s) 162, 1371, 1726, 1845
AfaI GTAC 1 cut(s) 292
AfeI AGCGCT 1 cut(s) 909
AfiI CCNNNNNNNGG 5 cut(s) 28, 224, 590, 713, 1820
AjnI CCWGG 6 cut(s) 84, 1012, 1228, 1464, 1525, 1548
AloI GAACNNNNNNTCC 2 cut(s) 565, 597
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 3 cut(s) 60, 1340, 1527
AlwI GGATC 3 cut(s) 235, 452, 1537
AlwNI CAGNNNCTG 1 cut(s) 976
Aor13HI TCCGGA 1 cut(s) 569
Aor51HI AGCGCT 1 cut(s) 909
AoxI GGCC 7 cut(s) 73, 273, 500, 1366, 1546, 1565, 1831
ApaI GGGCCC 1 cut(s) 504
ApeKI GCWGC 4 cut(s) 630, 1806, 1844, 1856
ApoI RAATTY 7 cut(s) 248, 648, 826, 883, 1252, 1289, 1773
AseI ATTAAT 1 cut(s) 923
Asp700I GAANNNNTTC 3 cut(s) 419, 1196, 1739
AspLEI GCGC 2 cut(s) 196, 910
AspS9I GGNCC 8 cut(s) 428, 500, 501, 788, 1367, 1565, 1800, 1832
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 656, 1201
AvaII GGWCC 3 cut(s) 428, 788, 1800
BaeGI GKGCMC 2 cut(s) 23, 504
BanI GGYRCC 2 cut(s) 18, 305
BanII GRGCYC 3 cut(s) 260, 504, 1081
BarI GAAGNNNNNNTAC 2 cut(s) 925, 957
BbsI GAAGAC 2 cut(s) 1139, 1359
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 4 cut(s) 617, 1793, 1831, 1843
BccI CCATC 4 cut(s) 145, 242, 284, 1924
BceAI ACGGC 3 cut(s) 129, 1658, 1846
BcgI CGANNNNNNTGC 2 cut(s) 1595, 1629
BciT130I CCWGG 6 cut(s) 86, 1014, 1230, 1466, 1527, 1550
BciVI GTATCC 2 cut(s) 172, 714
BclI TGATCA 1 cut(s) 1816
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 3 cut(s) 60, 1340, 1527
BfaI CTAG 4 cut(s) 1086, 1313, 1637, 1967
BfmI CTRYAG 2 cut(s) 1113, 1902
BfoI RGCGCY 2 cut(s) 197, 911
BfuAI ACCTGC 2 cut(s) 320, 1811
BfuI GTATCC 2 cut(s) 172, 714
BisI GCNGC 4 cut(s) 631, 1807, 1845, 1857
BlsI GCNGC 4 cut(s) 632, 1808, 1846, 1858
Bme1390I CCNGG 7 cut(s) 86, 272, 1014, 1230, 1466, 1527, 1550
Bme18I GGWCC 3 cut(s) 428, 788, 1800
BmgT120I GGNCC 8 cut(s) 428, 500, 501, 788, 1367, 1565, 1800, 1832
BmiI GGNNCC 8 cut(s) 6, 20, 216, 307, 502, 1673, 1801, 1944
BmrFI CCNGG 7 cut(s) 86, 272, 1014, 1230, 1466, 1527, 1550
BmsI GCATC 2 cut(s) 704, 945
BpiI GAAGAC 2 cut(s) 1139, 1359
BpmI CTGGAG 1 cut(s) 1509
BpuEI CTTGAG 4 cut(s) 168, 512, 1498, 1577
BpuMI CCSGG 1 cut(s) 272
Bsa29I ATCGAT 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 4 cut(s) 85, 1154, 1464, 1549
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 4 cut(s) 565, 595, 1944, 1974
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 224, 590, 713, 1820
Bse1I ACTGG 3 cut(s) 801, 934, 981
Bse3DI GCAATG 1 cut(s) 1420
BseAI TCCGGA 1 cut(s) 569
BseBI CCWGG 6 cut(s) 86, 1014, 1230, 1466, 1527, 1550
BseCI ATCGAT 1 cut(s) 408
BseDI CCNNGG 4 cut(s) 85, 1154, 1464, 1549
BseGI GGATG 1 cut(s) 936
BseLI CCNNNNNNNGG 5 cut(s) 28, 224, 590, 713, 1820
BseMI GCAATG 1 cut(s) 1420
BseMII CTCAG 4 cut(s) 320, 726, 1371, 1526
BseNI ACTGG 3 cut(s) 801, 934, 981
BseRI GAGGAG 2 cut(s) 369, 853
BseSI GKGCMC 2 cut(s) 23, 504
BseXI GCAGC 4 cut(s) 617, 1793, 1831, 1843
BseYI CCCAGC 2 cut(s) 50, 1369
BsgI GTGCAG 1 cut(s) 1792
BshFI GGCC 7 cut(s) 75, 275, 502, 1368, 1548, 1567, 1833
BshNI GGYRCC 2 cut(s) 18, 305
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 272, 570
BslFI GGGAC 1 cut(s) 1813
BslI CCNNNNNNNGG 5 cut(s) 28, 224, 590, 713, 1820
BsmAI GTCTC 3 cut(s) 60, 1340, 1527
BsmFI GGGAC 1 cut(s) 1813
BsmI GAATGC 1 cut(s) 491
BsnI GGCC 7 cut(s) 75, 275, 502, 1368, 1548, 1567, 1833
Bso31I GGTCTC 1 cut(s) 60
Bsp120I GGGCCC 1 cut(s) 500
Bsp1286I GDGCHC 4 cut(s) 23, 260, 504, 1081
Bsp13I TCCGGA 1 cut(s) 569
Bsp143I GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
Bsp19I CCATGG 1 cut(s) 1154
BspACI CCGC 3 cut(s) 188, 633, 673
BspANI GGCC 7 cut(s) 75, 275, 502, 1368, 1548, 1567, 1833
BspCNI CTCAG 4 cut(s) 321, 725, 1370, 1527
BspDI ATCGAT 1 cut(s) 408
BspEI TCCGGA 1 cut(s) 569
BspLI GGNNCC 8 cut(s) 6, 20, 216, 307, 502, 1673, 1801, 1944
BspMI ACCTGC 2 cut(s) 320, 1811
BspPI GGATC 3 cut(s) 235, 452, 1537
BspQI GCTCTTC 2 cut(s) 1086, 1907
BspT107I GGYRCC 2 cut(s) 18, 305
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 1420
BsrI ACTGG 3 cut(s) 801, 934, 981
BssECI CCNNGG 4 cut(s) 85, 1154, 1464, 1549
BssMI GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
BssT1I CCWWGG 1 cut(s) 1154
Bst2UI CCWGG 6 cut(s) 86, 1014, 1230, 1466, 1527, 1550
Bst4CI ACNGT 8 cut(s) 290, 302, 1211, 1304, 1576, 1600, 1879, 1903
Bst6I CTCTTC 3 cut(s) 68, 1086, 1907
BstAPI GCANNNNNTGC 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 1070, 1854
BstDEI CTNAG 5 cut(s) 329, 712, 1183, 1357, 1535
BstDSI CCRYGG 1 cut(s) 1154
BstEII GGTNACC 1 cut(s) 1159
BstENI CCTNNNNNAGG 1 cut(s) 1818
BstF5I GGATG 1 cut(s) 936
BstH2I RGCGCY 2 cut(s) 197, 911
BstHHI GCGC 2 cut(s) 196, 910
BstKTI GATC 6 cut(s) 230, 412, 447, 1532, 1612, 1819
BstMAI GTCTC 3 cut(s) 60, 1340, 1527
BstMBI GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
BstMWI GCNNNNNNNGC 5 cut(s) 122, 312, 488, 1627, 1853
BstNI CCWGG 6 cut(s) 86, 1014, 1230, 1466, 1527, 1550
BstNSI RCATGY 1 cut(s) 1856
BstPI GGTNACC 1 cut(s) 1159
BstSCI CCNGG 7 cut(s) 84, 270, 1012, 1228, 1464, 1525, 1548
BstSFI CTRYAG 2 cut(s) 1113, 1902
BstSLI GKGCMC 2 cut(s) 23, 504
BstV1I GCAGC 4 cut(s) 617, 1793, 1831, 1843
BstV2I GAAGAC 2 cut(s) 1139, 1359
Bsu15I ATCGAT 1 cut(s) 408
BsuI GTATCC 2 cut(s) 172, 714
BsuRI GGCC 7 cut(s) 75, 275, 502, 1368, 1548, 1567, 1833
BsuTUI ATCGAT 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 1154
BtsCI GGATG 1 cut(s) 936
BtsI GCAGTG 1 cut(s) 229
BtsIMutI CAGTG 6 cut(s) 214, 229, 307, 847, 1532, 1750
BveI ACCTGC 2 cut(s) 320, 1811
Cac8I GCNNGC 2 cut(s) 1070, 1854
CaiI CAGNNNCTG 1 cut(s) 976
CfoI GCGC 2 cut(s) 196, 910
Cfr13I GGNCC 8 cut(s) 428, 500, 501, 788, 1367, 1565, 1800, 1832
ClaI ATCGAT 1 cut(s) 408
Csp6I GTAC 1 cut(s) 291
CspCI CAANNNNNGTGG 2 cut(s) 1288, 1323
CviAII CATG 7 cut(s) 219, 441, 1030, 1155, 1260, 1618, 1853
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 5 cut(s) 329, 712, 1183, 1357, 1535
DpnI GATC 6 cut(s) 229, 411, 446, 1531, 1611, 1818
DpnII GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
DrdI GACNNNNNNGTC 1 cut(s) 472
DseDI GACNNNNNNGTC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 3 cut(s) 68, 1086, 1907
EarI CTCTTC 3 cut(s) 68, 1086, 1907
Ecl136II GAGCTC 1 cut(s) 1079
Eco130I CCWWGG 1 cut(s) 1154
Eco24I GRGCYC 3 cut(s) 260, 504, 1081
Eco31I GGTCTC 1 cut(s) 60
Eco32I GATATC 2 cut(s) 847, 1657
Eco47I GGWCC 3 cut(s) 428, 788, 1800
Eco47III AGCGCT 1 cut(s) 909
Eco53kI GAGCTC 1 cut(s) 1079
Eco57I CTGAAG 4 cut(s) 162, 1371, 1726, 1845
Eco91I GGTNACC 1 cut(s) 1159
EcoICRI GAGCTC 1 cut(s) 1079
EcoNI CCTNNNNNAGG 1 cut(s) 1818
EcoO109I RGGNCCY 2 cut(s) 428, 1800
EcoO65I GGTNACC 1 cut(s) 1159
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 6 cut(s) 84, 1012, 1228, 1464, 1525, 1548
EcoRV GATATC 2 cut(s) 847, 1657
EcoT14I CCWWGG 1 cut(s) 1154
EcoT22I ATGCAT 2 cut(s) 493, 922
EcoT38I GRGCYC 3 cut(s) 260, 504, 1081
ErhI CCWWGG 1 cut(s) 1154
FaeI CATG 7 cut(s) 222, 444, 1033, 1158, 1263, 1621, 1856
FaqI GGGAC 1 cut(s) 1813
FatI CATG 7 cut(s) 218, 440, 1029, 1154, 1259, 1617, 1852
FbaI TGATCA 1 cut(s) 1816
Fnu4HI GCNGC 4 cut(s) 631, 1807, 1845, 1857
FokI GGATG 1 cut(s) 923
FriOI GRGCYC 3 cut(s) 260, 504, 1081
Fsp4HI GCNGC 4 cut(s) 631, 1807, 1845, 1857
FspBI CTAG 4 cut(s) 1086, 1313, 1637, 1967
GlaI GCGC 2 cut(s) 195, 909
GluI GCNGC 4 cut(s) 631, 1807, 1845, 1857
GsaI CCCAGC 2 cut(s) 54, 1373
GsuI CTGGAG 1 cut(s) 1509
HaeII RGCGCY 2 cut(s) 197, 911
HaeIII GGCC 7 cut(s) 75, 275, 502, 1368, 1548, 1567, 1833
HapII CCGG 2 cut(s) 272, 570
HhaI GCGC 2 cut(s) 196, 910
Hin1II CATG 7 cut(s) 222, 444, 1033, 1158, 1263, 1621, 1856
Hin6I GCGC 2 cut(s) 194, 908
HinP1I GCGC 2 cut(s) 194, 908
HincII GTYRAC 1 cut(s) 1007
HindII GTYRAC 1 cut(s) 1007
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 5 cut(s) 333, 636, 837, 1355, 1441
HpaII CCGG 2 cut(s) 272, 570
HphI GGTGA 2 cut(s) 656, 1201
Hpy166II GTNNAC 4 cut(s) 968, 1007, 1192, 1310
Hpy188I TCNGA 9 cut(s) 142, 414, 510, 700, 946, 1396, 1446, 1759, 1864
Hpy188III TCNNGA 9 cut(s) 400, 570, 584, 714, 869, 1140, 1507, 1556, 1769
Hpy8I GTNNAC 4 cut(s) 968, 1007, 1192, 1310
HpyCH4III ACNGT 8 cut(s) 290, 302, 1211, 1304, 1576, 1600, 1879, 1903
HpyCH4IV ACGT 1 cut(s) 1376
HpyF10VI GCNNNNNNNGC 5 cut(s) 122, 312, 488, 1627, 1853
HpyF3I CTNAG 5 cut(s) 329, 712, 1183, 1357, 1535
HpySE526I ACGT 1 cut(s) 1376
Hsp92II CATG 7 cut(s) 222, 444, 1033, 1158, 1263, 1621, 1856
HspAI GCGC 2 cut(s) 194, 908
Kpn2I TCCGGA 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 1816
Kzo9I GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
LguI GCTCTTC 2 cut(s) 1086, 1907
LmnI GCTCC 5 cut(s) 10, 214, 514, 866, 1076
Lsp1109I GCAGC 4 cut(s) 617, 1793, 1831, 1843
LweI GCATC 2 cut(s) 704, 945
MaeI CTAG 4 cut(s) 1086, 1313, 1637, 1967
MaeII ACGT 1 cut(s) 1376
MaeIII GTNAC 5 cut(s) 89, 1159, 1170, 1384, 1489
MalI GATC 6 cut(s) 229, 411, 446, 1531, 1611, 1818
MboI GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
MboII GAAGA 8 cut(s) 55, 901, 1073, 1097, 1139, 1217, 1364, 1924
MfeI CAATTG 1 cut(s) 477
MhlI GDGCHC 4 cut(s) 23, 260, 504, 1081
MlyI GAGTC 3 cut(s) 342, 630, 1349
MmeI TCCRAC 1 cut(s) 1930
Mph1103I ATGCAT 2 cut(s) 493, 922
MroI TCCGGA 1 cut(s) 569
MroXI GAANNNNTTC 3 cut(s) 419, 1196, 1739
MseI TTAA 8 cut(s) 264, 536, 879, 923, 998, 1235, 1721, 1749
MspA1I CMGCKG 2 cut(s) 630, 1177
MspI CCGG 2 cut(s) 272, 570
MspR9I CCNGG 7 cut(s) 86, 272, 1014, 1230, 1466, 1527, 1550
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 491
MvaI CCWGG 6 cut(s) 86, 1014, 1230, 1466, 1527, 1550
MwoI GCNNNNNNNGC 5 cut(s) 122, 312, 488, 1627, 1853
NciI CCSGG 1 cut(s) 272
NcoI CCATGG 1 cut(s) 1154
NdeII GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
NlaIII CATG 7 cut(s) 222, 444, 1033, 1158, 1263, 1621, 1856
NlaIV GGNNCC 8 cut(s) 6, 20, 216, 307, 502, 1673, 1801, 1944
NmuCI GTSAC 2 cut(s) 1170, 1489
NsiI ATGCAT 2 cut(s) 493, 922
NspI RCATGY 1 cut(s) 1856
PaeI GCATGC 1 cut(s) 1856
PaqCI CACCTGC 1 cut(s) 320
PciSI GCTCTTC 2 cut(s) 1086, 1907
PctI GAATGC 1 cut(s) 491
PdmI GAANNNNTTC 3 cut(s) 419, 1196, 1739
PfeI GAWTC 2 cut(s) 837, 1441
PflMI CCANNNNNTGG 1 cut(s) 224
PfoI TCCNGGA 2 cut(s) 1228, 1525
PkrI GCNGC 4 cut(s) 632, 1808, 1846, 1858
PleI GAGTC 3 cut(s) 341, 630, 1349
PpsI GAGTC 3 cut(s) 341, 630, 1349
PpuMI RGGWCCY 2 cut(s) 428, 1800
PshBI ATTAAT 1 cut(s) 923
PsiI TTATAA 1 cut(s) 1973
Psp124BI GAGCTC 1 cut(s) 1081
Psp1406I AACGTT 1 cut(s) 1376
Psp5II RGGWCCY 2 cut(s) 428, 1800
Psp6I CCWGG 6 cut(s) 84, 1012, 1228, 1464, 1525, 1548
PspEI GGTNACC 1 cut(s) 1159
PspFI CCCAGC 2 cut(s) 50, 1369
PspGI CCWGG 6 cut(s) 84, 1012, 1228, 1464, 1525, 1548
PspN4I GGNNCC 8 cut(s) 6, 20, 216, 307, 502, 1673, 1801, 1944
PspOMI GGGCCC 1 cut(s) 500
PspPI GGNCC 8 cut(s) 428, 500, 501, 788, 1367, 1565, 1800, 1832
PspPPI RGGWCCY 2 cut(s) 428, 1800
PstNI CAGNNNCTG 1 cut(s) 976
PvuII CAGCTG 2 cut(s) 630, 1177
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SacI GAGCTC 1 cut(s) 1081
SapI GCTCTTC 2 cut(s) 1086, 1907
SaqAI TTAA 8 cut(s) 264, 536, 879, 923, 998, 1235, 1721, 1749
SatI GCNGC 4 cut(s) 631, 1807, 1845, 1857
Sau3AI GATC 6 cut(s) 227, 409, 444, 1529, 1609, 1816
Sau96I GGNCC 8 cut(s) 428, 500, 501, 788, 1367, 1565, 1800, 1832
SchI GAGTC 3 cut(s) 342, 630, 1349
ScrFI CCNGG 7 cut(s) 86, 272, 1014, 1230, 1466, 1527, 1550
SduI GDGCHC 4 cut(s) 23, 260, 504, 1081
SfaNI GCATC 2 cut(s) 704, 945
SfcI CTRYAG 2 cut(s) 1113, 1902
SinI GGWCC 3 cut(s) 428, 788, 1800
SmlI CTYRAG 4 cut(s) 183, 527, 1513, 1556
SmoI CTYRAG 4 cut(s) 183, 527, 1513, 1556
SphI GCATGC 1 cut(s) 1856
SsiI CCGC 3 cut(s) 188, 633, 673
SspI AATATT 1 cut(s) 165
SspMI CTAG 4 cut(s) 1086, 1313, 1637, 1967
SstI GAGCTC 1 cut(s) 1081
StyD4I CCNGG 7 cut(s) 84, 270, 1012, 1228, 1464, 1525, 1548
StyI CCWWGG 1 cut(s) 1154
TaaI ACNGT 8 cut(s) 290, 302, 1211, 1304, 1576, 1600, 1879, 1903
TaiI ACGT 1 cut(s) 1379
TaqI TCGA 3 cut(s) 148, 408, 1605
TatI WGTACW 1 cut(s) 290
TfiI GAWTC 2 cut(s) 837, 1441
Tru1I TTAA 8 cut(s) 264, 536, 879, 923, 998, 1235, 1721, 1749
Tru9I TTAA 8 cut(s) 264, 536, 879, 923, 998, 1235, 1721, 1749
TscAI CASTG 6 cut(s) 214, 229, 307, 847, 1539, 1750
TseFI GTSAC 2 cut(s) 1170, 1489
TseI GCWGC 4 cut(s) 630, 1806, 1844, 1856
Tsp45I GTSAC 2 cut(s) 1170, 1489
TspDTI ATGAA 4 cut(s) 721, 865, 1766, 1925
TspRI CASTG 6 cut(s) 214, 229, 307, 847, 1539, 1750
Van91I CCANNNNNTGG 1 cut(s) 224
VpaK11BI GGWCC 3 cut(s) 428, 788, 1800
VspI ATTAAT 1 cut(s) 923
XagI CCTNNNNNAGG 1 cut(s) 1818
XapI RAATTY 7 cut(s) 248, 648, 826, 883, 1252, 1289, 1773
XceI RCATGY 1 cut(s) 1856
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XmnI GAANNNNTTC 3 cut(s) 419, 1196, 1739
XspI CTAG 4 cut(s) 1086, 1313, 1637, 1967
Zsp2I ATGCAT 2 cut(s) 493, 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.