MD05G1257000.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000088
Physical Location & Seq
Forward (+)
38793726 .. 38801226
7501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1257000.v1.1.491

Sequence Viewer

Length: 2985 bp
ATGTGGGATTTCAACAAAAATATGGTTATGTTTGAAATCGATGAGAATTATTTCACTGGTCCCCTACCGGCATTCATTGGCAATATGTCTGCATTGACTGGATTGTCAATTGGCGGCAATTCATTCTCTGGGCCCATCCCCAAGGAGCTTGGAAACCTTAAGGAGCTAACATATTTTTACATGGACAGCTGTGGACTCGGTGGTGAAATTCCTTCAACATTTGCCAACCTCACCAACATGCAAATCCCCTGGGCATCGGACAGTCCTTTCTCAGGAAAGATACCTGATTTCATAGGGAATTGGACACAACTCACTGATTTGCAATTTCAAGGGAACTCTTTCGAAGGCCCAATACCAACCAGCTTTTCTCAACTGACCTCATTGAAATCTCTGCGAATCGGTGATATATACAATGGGAGCTCTTCTCTTGATTTCATAAAAAATCTGAAAAACTTGACTGATTTAAAACTGCGAAACACATTAATCACTGGTACCATCCCATCTGATATTGGAGAATATCAAAGTCTACAGATACTGGATCTCAGTTTCAACAATTTGACAGGCCAACTCCCAAGTTATTTGTTCATCTTGAGTTCTCTTACATCCTTGTTTCTTGGAAACAATAGTCTCTCCGGACCTCTTCCGAGCCAAAAGAGCTATGAACTTCAGACTATAGATTTGTCTTACAATTTTTTATCAGGAAGCTTTCCCCAGTGGGTGACCACAACATCGCAACTGAACTTAGTGGTCAACAACTTCACATTTGACAAAAAAAACATAACTCTTCCTGGATTGAATTGCCTCCAGAGAAATTTTCCATGCAATCGAAATGCCCCACGATGTAATCTGCTCTTATGTCACCCATCATTTACTATTTTAACGCAATGTCATGTTAGAGGAAAAGGCATTGCTTCTCTAGTTATGTATACATATGGATATAGATTGCAAGCATGTTTTCATAAGTTCAGTAATACGCATAACAGGTTAGAAATATATGCAAACTTCTCAATCAACTGTGGTGGACCGCAAATGACGGGAAGTGATGGCATATTGTATGAGGCTGAAGACTCAACTCTTGGCCCAGCAACATTCAGTGTAACCAGTACAGAGAAATGGGTTGTCAGCAATGCCGGTTTGTTTTATGAAAGAAAAAACCAATCGTTTCTGCAAAATACCCTTGCACAAGTCACTGGAACAAATGTGACCCCGGAGCTTTTCCAGACTTCAAGAGTGTCCCCAGGATCACTAAGATACTATGGCCTGGGCCTTGAGAATGGGCCTTACACTGTAACATTGCACTTTGCAGAGACGGTTTATGAAAGTCGCACTTCGCAAAAATGGGAAAGTCTAGGACGACGTGTATTTGATATCTATATTCAAGGTACCCGCAGGACGAAGGACTTTGACATATCCAAGGAGGCAGGTGGGGCTAAACTAGGAATTATGAAAAGATTCAATGTTAGCGTGTCAGAGAATTATCTTGAAATTCATCTGTTCTGGGCTGGTAAAGGGACTTATTGGATACCCGAGAAGGGTTATTACGGCCCACTAATAGCAGCCGTCCATGCTGCTTCAGATTTTACACCAACAGTTTCCGGGCTTCCACCAACTACTCCAGGAAAGAAGAGCAGGACTGGGTTGATAGTTGGTATTGCAATTCCTGTTGGAGCTGTGAGCTTGCTATTAATATTTGCGATTCTATATATGAGGAAAAAATCAGAAAAAGAGGATGACGAAGATATTCTAGGATTAGGCCCTCAACCAAATACTTTCAGTTATGTTGAGTTGAGAGCTGCAACCGAAGATTTTAATCCTTCAAATAAGTTAGGAGAGGGAGGATATGGCCCTGTTTATAAGGGTACACTTTTTGATGGGAGAGTAGTGGCTGTTAAGCAACTTTCAGTAGCATCTCACCAAGGGAACAGTCAATTTGTATCTGAAATTGCTACCATATCTGCTGTGCAACATCGGAATCTAGTGAAATTGTATGGATGCTGCATCGAAGGCAGCCACCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAGGCACTTTTTGGAACAAGTAACTTGCACCTTGACTGGCCTACTCGATTCAATATATTGTTGGGAACAACAAGAGGACTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCGAGTAATATTTTGCTCGATGCAAAACTCTCCCCAATAATATCAGATTTTGGACTGGCAAAACTTTATGATGACGAGAAAACCCACATCAGCACTCGGGTTGCAGGGACAATAGGCTATTTGGCACCGGAGTATGCAATGTTTGGACATTTGACAGAGAAGGCCGATGTGTTTGGTTTTGGAGTCGTCGTTTTAGAGATCCTCAGCGGGAGACCAAACTCTTACAATAACTTGGATCCAGAAAAGATTTATCTTCTTGAATGGGGATCACCGATGGCACGACCATCTATGTCACGTGTGGTTGCAATGCTCTCTGGAGACATTGACATAAGCACTGTCATGTCGAAGCCAAGCTATTTGACAGATTATGATTTTAAAGGTGTAACAACATCGTTAACAAGTAGATTTTTGGTGGAGGATGATACCCCATCAACTTCATCCAAGGGTAGTAATGTTCGCCTCAACTATCAATCTGGAGGCAGCAATGCAAGTGCAAGAGGACTTGCTTACCTCCATGAGGAGTCAAGGCCAAGGATTGTACATCAAGATGTCAAAGCCATTAATATTTTGCTCGATGCAGAACTCCCCCCAAAAATATCAGATTTTGGATTGGCAAAGCTTTATGATGGCGAGAAAACCCACATCAGCACCTGGGTTGCAAGGACAATAGGCTATTTGGCACCCGAGTATGCAATGCGTGGACATTTGATAGAGAAGGCTGATGTGTTTGGTTTTGGAGCTGTTGCTTTGGAGATCCCAAGCAGGAGACCAAACTCTGACAATAACTTAGATCCATAA

Protein Analysis

995

Amino Acids

109.87

Weight (kDa)

6.29

Isoelectric Point (pI)

36.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 123 - 209 9.8e-08 Leucine-rich repeat region
Malectin PF11721 335 - 521 2.2e-37 Malectin domain
Pkinase PF00069 605 - 806 4.1e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 606 - 825 3.7e-42 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 892 - 983 5.3e-15 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 894 - 986 4.3e-16 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1854
AarI CACCTGC 1 cut(s) 1412
AasI GACNNNNNNGTC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 1412
Acc65I GGTACC 2 cut(s) 491, 1382
AccB1I GGYRCC 4 cut(s) 491, 1382, 2311, 2866
AccI GTMKAC 2 cut(s) 526, 926
AccIII TCCGGA 1 cut(s) 632
AciI CCGC 5 cut(s) 114, 1025, 1387, 2014, 2394
AclWI GGATC 8 cut(s) 546, 1249, 2380, 2417, 2430, 2461, 2935, 2972
AcsI RAATTY 3 cut(s) 207, 811, 1485
AcuI CTGAAG 3 cut(s) 650, 1083, 1557
AcvI CACGTG 1 cut(s) 2483
AfaI GTAC 6 cut(s) 493, 1105, 1384, 1861, 2172, 2727
AfiI CCNNNNNNNGG 6 cut(s) 272, 1532, 2020, 2056, 2149, 2704
AflII CTTAAG 1 cut(s) 158
AflIII ACRYGT 2 cut(s) 1357, 2482
AjiI CACGTC 1 cut(s) 1358
AjnI CCWGG 6 cut(s) 248, 787, 1237, 1260, 1615, 2837
AjuI GAANNNNNNNTTGG 2 cut(s) 349, 381
Alw21I GWGCWC 1 cut(s) 422
Alw26I GTCTC 5 cut(s) 632, 1301, 2392, 2499, 2947
AlwI GGATC 8 cut(s) 546, 1249, 2380, 2417, 2430, 2461, 2935, 2972
AlwNI CAGNNNCTG 2 cut(s) 535, 2838
Ama87I CYCGRG 3 cut(s) 1526, 2283, 2870
Aor13HI TCCGGA 1 cut(s) 632
ApaI GGGCCC 1 cut(s) 135
ApeKI GCWGC 6 cut(s) 1556, 1568, 1793, 1995, 2007, 2667
ApoI RAATTY 3 cut(s) 207, 811, 1485
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
AseI ATTAAT 3 cut(s) 482, 1685, 2748
Asp700I GAANNNNTTC 4 cut(s) 50, 338, 1451, 1740
Asp718I GGTACC 2 cut(s) 491, 1382
AsuC2I CCSGG 2 cut(s) 1208, 1597
AsuHPI GGTGA 7 cut(s) 215, 223, 413, 730, 851, 1904, 2448
AsuII TTCGAA 1 cut(s) 342
AvaI CYCGRG 3 cut(s) 1526, 2283, 2870
AvaII GGWCC 3 cut(s) 59, 635, 1022
BaeGI GKGCMC 1 cut(s) 135
BamHI GGATCC 1 cut(s) 2422
BanI GGYRCC 4 cut(s) 491, 1382, 2311, 2866
BanII GRGCYC 2 cut(s) 135, 422
BarI GAAGNNNNNNTAC 4 cut(s) 336, 368, 1523, 1555
BbrPI CACGTG 1 cut(s) 2483
BbsI GAAGAC 1 cut(s) 1071
Bbv12I GWGCWC 1 cut(s) 422
BbvCI CCTCAGC 1 cut(s) 2390
BbvI GCAGC 6 cut(s) 1555, 1568, 1780, 1982, 2019, 2679
BceAI ACGGC 2 cut(s) 1544, 1558
BciT130I CCWGG 6 cut(s) 250, 789, 1239, 1262, 1617, 2839
BciVI GTATCC 1 cut(s) 1515
BclI TGATCA 1 cut(s) 2053
BcnI CCSGG 2 cut(s) 1208, 1597
BcoDI GTCTC 5 cut(s) 632, 1301, 2392, 2499, 2947
BfaI CTAG 5 cut(s) 917, 1349, 1436, 1745, 1976
BfmI CTRYAG 2 cut(s) 527, 672
BfrI CTTAAG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 1412
BfuI GTATCC 1 cut(s) 1515
BisI GCNGC 7 cut(s) 115, 1557, 1569, 1794, 1996, 2008, 2668
BlsI GCNGC 7 cut(s) 116, 1558, 1570, 1795, 1997, 2009, 2669
Bme1390I CCNGG 8 cut(s) 250, 789, 1208, 1239, 1262, 1597, 1617, 2839
Bme18I GGWCC 3 cut(s) 59, 635, 1022
BmeT110I CYCGRG 3 cut(s) 1526, 2283, 2870
BmgBI CACGTC 1 cut(s) 1358
BmiI GGNNCC 7 cut(s) 61, 133, 493, 1384, 2313, 2424, 2868
BmrFI CCNGG 8 cut(s) 250, 789, 1208, 1239, 1262, 1597, 1617, 2839
BmrI ACTGGG 2 cut(s) 706, 1644
BmsI GCATC 6 cut(s) 263, 1916, 1982, 2007, 2197, 2752
BmuI ACTGGG 2 cut(s) 706, 1644
BpiI GAAGAC 1 cut(s) 1071
BplI GAGNNNNNCTC 2 cut(s) 409, 441
BpmI CTGGAG 4 cut(s) 788, 1599, 2523, 2682
Bpu10I CCTNAGC 1 cut(s) 2390
Bpu14I TTCGAA 1 cut(s) 342
BpuEI CTTGAG 2 cut(s) 610, 1289
BpuMI CCSGG 2 cut(s) 1208, 1597
Bsa29I ATCGAT 1 cut(s) 39
BsaAI YACGTR 1 cut(s) 2483
BsaBI GATNNNNATC 1 cut(s) 1809
BsaI GGTCTC 2 cut(s) 2392, 2947
BsaWI WCCGGW 2 cut(s) 632, 2314
BsaXI ACNNNNNCTCC 2 cut(s) 2361, 2391
Bsc4I CCNNNNNNNGG 6 cut(s) 272, 1532, 2020, 2056, 2149, 2704
Bse118I RCCGGY 2 cut(s) 67, 1130
Bse3DI GCAATG 8 cut(s) 890, 906, 1132, 1292, 2331, 2499, 2677, 2886
Bse8I GATNNNNATC 1 cut(s) 1809
BseAI TCCGGA 1 cut(s) 632
BseBI CCWGG 6 cut(s) 250, 789, 1239, 1262, 1617, 2839
BseCI ATCGAT 1 cut(s) 39
BseGI GGATG 7 cut(s) 135, 495, 602, 1735, 1997, 2611, 2624
BseJI GATNNNNATC 1 cut(s) 1809
BseLI CCNNNNNNNGG 6 cut(s) 272, 1532, 2020, 2056, 2149, 2704
BseMI GCAATG 8 cut(s) 890, 906, 1132, 1292, 2331, 2499, 2677, 2886
BseMII CTCAG 3 cut(s) 285, 556, 2404
BseRI GAGGAG 2 cut(s) 2165, 2720
BseSI GKGCMC 1 cut(s) 135
BseXI GCAGC 6 cut(s) 1555, 1568, 1780, 1982, 2019, 2679
BseYI CCCAGC 1 cut(s) 1081
BshNI GGYRCC 4 cut(s) 491, 1382, 2311, 2866
BshVI ATCGAT 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 422
BsiHKCI CYCGRG 3 cut(s) 1526, 2283, 2870
BsiSI CCGG 6 cut(s) 68, 633, 1131, 1208, 1596, 2315
BslFI GGGAC 4 cut(s) 45, 1219, 1525, 2308
BslI CCNNNNNNNGG 6 cut(s) 272, 1532, 2020, 2056, 2149, 2704
BsmAI GTCTC 5 cut(s) 632, 1301, 2392, 2499, 2947
BsmBI CGTCTC 1 cut(s) 1301
BsmFI GGGAC 4 cut(s) 45, 1219, 1525, 2308
BsmI GAATGC 1 cut(s) 71
Bso31I GGTCTC 2 cut(s) 2392, 2947
BsoBI CYCGRG 3 cut(s) 1526, 2283, 2870
Bsp119I TTCGAA 1 cut(s) 342
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 135, 422
Bsp13I TCCGGA 1 cut(s) 632
Bsp1407I TGTACA 2 cut(s) 2170, 2725
Bsp143I GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
BspACI CCGC 5 cut(s) 114, 1025, 1387, 2014, 2394
BspCNI CTCAG 3 cut(s) 284, 555, 2403
BspDI ATCGAT 1 cut(s) 39
BspEI TCCGGA 1 cut(s) 632
BspHI TCATGA 1 cut(s) 2146
BspLI GGNNCC 7 cut(s) 61, 133, 493, 1384, 2313, 2424, 2868
BspMI ACCTGC 1 cut(s) 1412
BspPI GGATC 8 cut(s) 546, 1249, 2380, 2417, 2430, 2461, 2935, 2972
BspQI GCTCTTC 2 cut(s) 427, 1619
BspT104I TTCGAA 1 cut(s) 342
BspT107I GGYRCC 4 cut(s) 491, 1382, 2311, 2866
BspTI CTTAAG 1 cut(s) 158
BspTNI GGTCTC 2 cut(s) 2392, 2947
BsrDI GCAATG 8 cut(s) 890, 906, 1132, 1292, 2331, 2499, 2677, 2886
BsrFI RCCGGY 2 cut(s) 67, 1130
BsrGI TGTACA 2 cut(s) 2170, 2725
BssAI RCCGGY 2 cut(s) 67, 1130
BssMI GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
BssNAI GTATAC 1 cut(s) 927
BssT1I CCWWGG 6 cut(s) 141, 1413, 1915, 2162, 2628, 2717
Bst1107I GTATAC 1 cut(s) 927
Bst2UI CCWGG 6 cut(s) 250, 789, 1239, 1262, 1617, 2839
Bst4CI ACNGT 7 cut(s) 263, 1016, 1288, 1312, 1591, 1925, 2524
Bst6I CTCTTC 4 cut(s) 427, 645, 789, 1619
BstAFI CTTAAG 1 cut(s) 158
BstAUI TGTACA 2 cut(s) 2170, 2725
BstBAI YACGTR 1 cut(s) 2483
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 948, 1679
BstDEI CTNAG 6 cut(s) 271, 542, 742, 1247, 2390, 2974
BstEII GGTNACC 1 cut(s) 718
BstENI CCTNNNNNAGG 4 cut(s) 270, 2054, 2147, 2702
BstF5I GGATG 7 cut(s) 135, 495, 602, 1735, 1997, 2611, 2624
BstKTI GATC 8 cut(s) 541, 1244, 2056, 2388, 2425, 2456, 2943, 2980
BstMAI GTCTC 5 cut(s) 632, 1301, 2392, 2499, 2947
BstMBI GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
BstMWI GCNNNNNNNGC 5 cut(s) 654, 1427, 1565, 2004, 2013
BstNI CCWGG 6 cut(s) 250, 789, 1239, 1262, 1617, 2839
BstNSI RCATGY 2 cut(s) 241, 954
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 8 cut(s) 248, 787, 1206, 1237, 1260, 1595, 1615, 2837
BstSFI CTRYAG 2 cut(s) 527, 672
BstSLI GKGCMC 1 cut(s) 135
BstV1I GCAGC 6 cut(s) 1555, 1568, 1780, 1982, 2019, 2679
BstV2I GAAGAC 1 cut(s) 1071
BstX2I RGATCY 5 cut(s) 538, 2385, 2422, 2940, 2977
BstYI RGATCY 5 cut(s) 538, 2385, 2422, 2940, 2977
BstZ17I GTATAC 1 cut(s) 927
Bsu15I ATCGAT 1 cut(s) 39
BsuI GTATCC 1 cut(s) 1515
BsuTUI ATCGAT 1 cut(s) 39
BtgZI GCGATG 1 cut(s) 714
BtrI CACGTC 1 cut(s) 1358
BtsCI GGATG 7 cut(s) 135, 495, 602, 1735, 1997, 2611, 2624
BtsIMutI CAGTG 8 cut(s) 54, 312, 486, 719, 1099, 1188, 1284, 2520
BveI ACCTGC 1 cut(s) 1412
Cac8I GCNNGC 2 cut(s) 948, 1679
CaiI CAGNNNCTG 2 cut(s) 535, 2838
CciI TCATGA 1 cut(s) 2146
Cfr10I RCCGGY 2 cut(s) 67, 1130
ClaI ATCGAT 1 cut(s) 39
Csp6I GTAC 6 cut(s) 492, 1104, 1383, 1860, 2171, 2726
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviAII CATG 9 cut(s) 181, 238, 819, 890, 951, 1565, 2147, 2527, 2702
CviQI GTAC 6 cut(s) 492, 1104, 1383, 1860, 2171, 2726
DdeI CTNAG 6 cut(s) 271, 542, 742, 1247, 2390, 2974
DpnI GATC 8 cut(s) 540, 1243, 2055, 2387, 2424, 2455, 2942, 2979
DpnII GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
DraI TTTAAA 2 cut(s) 465, 2563
DrdI GACNNNNNNGTC 1 cut(s) 103
DseDI GACNNNNNNGTC 1 cut(s) 103
Eam1104I CTCTTC 4 cut(s) 427, 645, 789, 1619
EarI CTCTTC 4 cut(s) 427, 645, 789, 1619
Ecl136II GAGCTC 1 cut(s) 420
Eco130I CCWWGG 6 cut(s) 141, 1413, 1915, 2162, 2628, 2717
Eco24I GRGCYC 2 cut(s) 135, 422
Eco31I GGTCTC 2 cut(s) 2392, 2947
Eco32I GATATC 1 cut(s) 1369
Eco47I GGWCC 3 cut(s) 59, 635, 1022
Eco53kI GAGCTC 1 cut(s) 420
Eco57I CTGAAG 3 cut(s) 650, 1083, 1557
Eco72I CACGTG 1 cut(s) 2483
Eco88I CYCGRG 3 cut(s) 1526, 2283, 2870
Eco91I GGTNACC 1 cut(s) 718
EcoICRI GAGCTC 1 cut(s) 420
EcoNI CCTNNNNNAGG 4 cut(s) 270, 2054, 2147, 2702
EcoO109I RGGNCCY 1 cut(s) 1754
EcoO65I GGTNACC 1 cut(s) 718
EcoRII CCWGG 6 cut(s) 248, 787, 1237, 1260, 1615, 2837
EcoRV GATATC 1 cut(s) 1369
EcoT14I CCWWGG 6 cut(s) 141, 1413, 1915, 2162, 2628, 2717
EcoT38I GRGCYC 2 cut(s) 135, 422
ErhI CCWWGG 6 cut(s) 141, 1413, 1915, 2162, 2628, 2717
Esp3I CGTCTC 1 cut(s) 1301
FaeI CATG 9 cut(s) 184, 241, 822, 893, 954, 1568, 2150, 2530, 2705
FalI AAGNNNNNCTT 2 cut(s) 1312, 1344
FaqI GGGAC 4 cut(s) 45, 1219, 1525, 2308
FatI CATG 9 cut(s) 180, 237, 818, 889, 950, 1564, 2146, 2526, 2701
FauI CCCGC 2 cut(s) 1394, 2387
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 1 cut(s) 2053
FblI GTMKAC 2 cut(s) 526, 926
Fnu4HI GCNGC 7 cut(s) 115, 1557, 1569, 1794, 1996, 2008, 2668
FokI GGATG 7 cut(s) 122, 482, 589, 1742, 2004, 2611, 2618
FriOI GRGCYC 2 cut(s) 135, 422
Fsp4HI GCNGC 7 cut(s) 115, 1557, 1569, 1794, 1996, 2008, 2668
FspBI CTAG 5 cut(s) 917, 1349, 1436, 1745, 1976
GluI GCNGC 7 cut(s) 115, 1557, 1569, 1794, 1996, 2008, 2668
GsaI CCCAGC 1 cut(s) 1085
GsuI CTGGAG 4 cut(s) 788, 1599, 2523, 2682
HapII CCGG 6 cut(s) 68, 633, 1131, 1208, 1596, 2315
Hin1II CATG 9 cut(s) 184, 241, 822, 893, 954, 1568, 2150, 2530, 2705
HincII GTYRAC 2 cut(s) 751, 2583
HindII GTYRAC 2 cut(s) 751, 2583
HindIII AAGCTT 2 cut(s) 703, 2804
HpaI GTTAAC 1 cut(s) 2583
HpaII CCGG 6 cut(s) 68, 633, 1131, 1208, 1596, 2315
HphI GGTGA 7 cut(s) 215, 223, 413, 730, 851, 1904, 2448
Hpy166II GTNNAC 8 cut(s) 194, 527, 751, 927, 1022, 1862, 2583, 2888
Hpy8I GTNNAC 8 cut(s) 194, 527, 751, 927, 1022, 1862, 2583, 2888
Hpy99I CGWCG 2 cut(s) 1359, 2378
HpyAV CCTTC 9 cut(s) 222, 338, 1390, 1525, 1824, 1997, 2153, 2341, 2896
HpyCH4III ACNGT 7 cut(s) 263, 1016, 1288, 1312, 1591, 1925, 2524
HpyCH4IV ACGT 2 cut(s) 1357, 2482
HpyF10VI GCNNNNNNNGC 5 cut(s) 654, 1427, 1565, 2004, 2013
HpyF3I CTNAG 6 cut(s) 271, 542, 742, 1247, 2390, 2974
HpySE526I ACGT 2 cut(s) 1357, 2482
Hsp92II CATG 9 cut(s) 184, 241, 822, 893, 954, 1568, 2150, 2530, 2705
Kpn2I TCCGGA 1 cut(s) 632
KpnI GGTACC 2 cut(s) 495, 1386
Ksp22I TGATCA 1 cut(s) 2053
KspAI GTTAAC 1 cut(s) 2583
Kzo9I GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
LguI GCTCTTC 2 cut(s) 427, 1619
LmnI GCTCC 6 cut(s) 145, 163, 417, 1210, 1667, 2924
Lsp1109I GCAGC 6 cut(s) 1555, 1568, 1780, 1982, 2019, 2679
LweI GCATC 6 cut(s) 263, 1916, 1982, 2007, 2197, 2752
MaeI CTAG 5 cut(s) 917, 1349, 1436, 1745, 1976
MaeII ACGT 2 cut(s) 1357, 2482
MaeIII GTNAC 9 cut(s) 718, 857, 1096, 1186, 1201, 1288, 2075, 2478, 2569
MalI GATC 8 cut(s) 540, 1243, 2055, 2387, 2424, 2455, 2942, 2979
MboI GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
MboII GAAGA 8 cut(s) 414, 632, 776, 1076, 1636, 1748, 1814, 2432
MfeI CAATTG 1 cut(s) 108
MflI RGATCY 5 cut(s) 538, 2385, 2422, 2940, 2977
MhlI GDGCHC 2 cut(s) 135, 422
MlyI GAGTC 5 cut(s) 189, 1061, 2162, 2379, 2717
MmeI TCCRAC 1 cut(s) 1645
MroI TCCGGA 1 cut(s) 632
MroXI GAANNNNTTC 4 cut(s) 50, 338, 1451, 1740
MslI CAYNNNNRTG 4 cut(s) 236, 2178, 2525, 2733
MspA1I CMGCKG 2 cut(s) 189, 2394
MspCI CTTAAG 1 cut(s) 158
MspI CCGG 6 cut(s) 68, 633, 1131, 1208, 1596, 2315
MspR9I CCNGG 8 cut(s) 250, 789, 1208, 1239, 1262, 1597, 1617, 2839
MunI CAATTG 1 cut(s) 108
Mva1269I GAATGC 1 cut(s) 71
MvaI CCWGG 6 cut(s) 250, 789, 1239, 1262, 1617, 2839
MwoI GCNNNNNNNGC 5 cut(s) 654, 1427, 1565, 2004, 2013
NciI CCSGG 2 cut(s) 1208, 1597
NdeI CATATG 1 cut(s) 931
NdeII GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
NlaIII CATG 9 cut(s) 184, 241, 822, 893, 954, 1568, 2150, 2530, 2705
NlaIV GGNNCC 7 cut(s) 61, 133, 493, 1384, 2313, 2424, 2868
NmuCI GTSAC 5 cut(s) 718, 857, 1186, 1201, 2478
NspI RCATGY 2 cut(s) 241, 954
NspV TTCGAA 1 cut(s) 342
PagI TCATGA 1 cut(s) 2146
PaqCI CACCTGC 1 cut(s) 1412
PasI CCCWGGG 1 cut(s) 249
PciSI GCTCTTC 2 cut(s) 427, 1619
PctI GAATGC 1 cut(s) 71
PdmI GAANNNNTTC 4 cut(s) 50, 338, 1451, 1740
PfeI GAWTC 5 cut(s) 396, 1452, 1696, 1972, 2103
PfoI TCCNGGA 2 cut(s) 787, 1615
PkrI GCNGC 7 cut(s) 116, 1558, 1570, 1795, 1997, 2009, 2669
PleI GAGTC 5 cut(s) 189, 1061, 2161, 2378, 2716
PmaCI CACGTG 1 cut(s) 2483
PmlI CACGTG 1 cut(s) 2483
PpsI GAGTC 5 cut(s) 189, 1061, 2161, 2378, 2716
Ppu21I YACGTR 1 cut(s) 2483
PshBI ATTAAT 3 cut(s) 482, 1685, 2748
PsiI TTATAA 1 cut(s) 1854
Psp124BI GAGCTC 1 cut(s) 422
Psp6I CCWGG 6 cut(s) 248, 787, 1237, 1260, 1615, 2837
PspCI CACGTG 1 cut(s) 2483
PspEI GGTNACC 1 cut(s) 718
PspFI CCCAGC 1 cut(s) 1081
PspGI CCWGG 6 cut(s) 248, 787, 1237, 1260, 1615, 2837
PspN4I GGNNCC 7 cut(s) 61, 133, 493, 1384, 2313, 2424, 2868
PspOMI GGGCCC 1 cut(s) 131
PstNI CAGNNNCTG 2 cut(s) 535, 2838
PsuI RGATCY 5 cut(s) 538, 2385, 2422, 2940, 2977
PvuII CAGCTG 1 cut(s) 189
RsaI GTAC 6 cut(s) 493, 1105, 1384, 1861, 2172, 2727
RsaNI GTAC 6 cut(s) 492, 1104, 1383, 1860, 2171, 2726
RseI CAYNNNNRTG 4 cut(s) 236, 2178, 2525, 2733
SacI GAGCTC 1 cut(s) 422
SapI GCTCTTC 2 cut(s) 427, 1619
SatI GCNGC 7 cut(s) 115, 1557, 1569, 1794, 1996, 2008, 2668
Sau3AI GATC 8 cut(s) 538, 1241, 2053, 2385, 2422, 2453, 2940, 2977
SchI GAGTC 5 cut(s) 189, 1061, 2162, 2379, 2717
ScrFI CCNGG 8 cut(s) 250, 789, 1208, 1239, 1262, 1597, 1617, 2839
SduI GDGCHC 2 cut(s) 135, 422
SfaNI GCATC 6 cut(s) 263, 1916, 1982, 2007, 2197, 2752
SfcI CTRYAG 2 cut(s) 527, 672
SfuI TTCGAA 1 cut(s) 342
SinI GGWCC 3 cut(s) 59, 635, 1022
SmiMI CAYNNNNRTG 4 cut(s) 236, 2178, 2525, 2733
SmlI CTYRAG 3 cut(s) 158, 589, 1268
SmoI CTYRAG 3 cut(s) 158, 589, 1268
SsiI CCGC 5 cut(s) 114, 1025, 1387, 2014, 2394
SspI AATATT 3 cut(s) 1689, 2197, 2752
SspMI CTAG 5 cut(s) 917, 1349, 1436, 1745, 1976
SstI GAGCTC 1 cut(s) 422
StyD4I CCNGG 8 cut(s) 248, 787, 1206, 1237, 1260, 1595, 1615, 2837
StyI CCWWGG 6 cut(s) 141, 1413, 1915, 2162, 2628, 2717
TaaI ACNGT 7 cut(s) 263, 1016, 1288, 1312, 1591, 1925, 2524
TaiI ACGT 2 cut(s) 1360, 2485
TaqI TCGA 9 cut(s) 39, 342, 826, 2001, 2101, 2176, 2205, 2531, 2760
TatI WGTACW 3 cut(s) 1103, 2170, 2725
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 5 cut(s) 396, 1452, 1696, 1972, 2103
TscAI CASTG 8 cut(s) 61, 319, 493, 719, 1099, 1195, 1291, 2527
TseFI GTSAC 5 cut(s) 718, 857, 1186, 1201, 2478
TseI GCWGC 6 cut(s) 1556, 1568, 1793, 1995, 2007, 2667
Tsp45I GTSAC 5 cut(s) 718, 857, 1186, 1201, 2478
TspRI CASTG 8 cut(s) 61, 319, 493, 719, 1099, 1195, 1291, 2527
Vha464I CTTAAG 1 cut(s) 158
VpaK11BI GGWCC 3 cut(s) 59, 635, 1022
VspI ATTAAT 3 cut(s) 482, 1685, 2748
XagI CCTNNNNNAGG 4 cut(s) 270, 2054, 2147, 2702
XapI RAATTY 3 cut(s) 207, 811, 1485
XceI RCATGY 2 cut(s) 241, 954
XmiI GTMKAC 2 cut(s) 526, 926
XmnI GAANNNNTTC 4 cut(s) 50, 338, 1451, 1740
XspI CTAG 5 cut(s) 917, 1349, 1436, 1745, 1976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.