MD05G1260700.v1.1

Di-glucose binding within endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000088
Physical Location & Seq
Reverse (-)
39578900 .. 39589574
10675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1260700.v1.1.491

Sequence Viewer

Length: 3111 bp
ATGAAGATGAAGTTGATGATGAGGATACTATTACTATGCTTCCTCAGCTGCTTGTGTTTTCAGCTGTCCTTTGTTCAAAATGCTACCACTGACCCATCTGAAGTGAGGGCGTTGAACTTAATATTTGAACAATGGGACACACAAGCAATGTCGGGGCTGTGGAATATCAGCGGAAAACCCTGCAGTGGATCTGCCATCGACGGCACCGACATTGATAGCCCCAATATCAACCCAGCCATCGTTTGCAACTGTTCTTACGACAACAATGCTACATGCCACATCACCAAACTGAAAGTGCAAGCTCTGTACAAACGAGGGGTGTTTCACGAAGAACTTTTGGCTCTAAGATATCTCGCAGTTTTGAAAATCGATAAGAATTATTTCAAAGGTTCCCTACCGGCATTCGTTGGCAATATGTCTGCATTGATTGAATTGCAAATTGGCCACAATTCATTCTCTGGGCCCATCCCCAAGGAGCTTGGAAACCTTAAGGAGCTAAGTATACTGGGCTTCGGATCAAATAATTTCTCCGGAACACTCCCTCCAGAACTTGGTAATTTAGTCAAGCTCCAGCAAATTTACATGGACAGCTGTGGAGTCAGTGGTGAAATTCCTTCAACATTTGCCAAGCTAACCAGTATGCAAATCTTTTGGACATCAGACAGTCTTTTCTCAGGAAAGATTCCTGATTTCATAGGGAATTGGACACAACTCATTTCTTTGCGATTTCAAGGGAACTCTTTGGAAGGCCCAATACCAACCAGCTTTTCTCAACTGACCTCATTGAAATCTCTGCATATCAATGATATATACGATGGGAGCTCCTCTCTTGATTTCATTAAAAATATGAAGAACTTGACTGAATTAAAACTACGAAACGCATTAATCACTGGTACCATCCCATCTGATATTGGAGAATATCAAAGTCTACAGATACTGGATCTGAGTTTCAACAATTTGACAGGCCAACTCCCAAGTTCTTTGTTCACCATGAGTTCTCTTACATCCTTGTTTCTTGGAAATAATAGTCTGTCCGGACCTCTTCCGAGCCAAAAGAGCGATCGACTTCAGACTATCTTTCCCCAGTGGGTGATCACAATATCGCAACTGAACTTAGTGGTCAACAACTTCACATTTGACGAAAAAAACAAAACTACTCTTCCTGGATTGAATTGCCTCCAGAGAAATTTTCCATGCAATCGAAATGCCCCACGATATGCAAACTTCTCAATCAACTGTGGTGGACCGGAAATGAAGGGACGTGATGGCATATCGTATGAGGCCGAAGACTCAGCTCTTGGTCCAGCAACATTCAATGTAACCAGTATAGAGAATTGGGCTGTCAGCAATGTCGGTATGTTTTCTGACGGAGTGAACCGATCGTTTCTGGAAAATACCCTTGAGCAAGTCACCGGAACAGATGTGACCCCGGAGCTTTTCCAGACTTCAAGATTGTCCCCAGGATCACTGAGATACTATGGCCTGGGTCTTGAGAATGGACCTTACACTGTAACGTTGCACTTTGCAGAGACGGTTTTTGATAGGCGCATTGAGCAAACTTGGGCAAGTCTAGGACGACGTGTGTTTGATATCTATATTCAGGGTACCCGCAGGACGAGGGACTTTGAAATATCCAAGGAGGCTGGTGGGGTTAAAATAGGAATTATGGAAAGATTCAATGTTAGCGTGTCAGAGAATTATCTTGAAATTCATCTGTTCTGGGCTGGTAAGGGGACTTGTTGCATACCCGATTTTGGTGATTACGGCCCACTAATAGCAGCTGTCCATGCTGCTTCAGATTTTACACCAACAGTTTCCGGGCTTCCACCAACTACTCGAGGAAAGAAAAGCAAGACTGGATTGATAGTTGGTATTGCAGTTCCTGTTGGAGTTGGGAGCTTGCTATTAATATTTGCGATTCTATATATGAGGAGGAAAAAATCAGAAAAAGAGGACGACGATGAAGATATTCTAGGATTAGGCCCTCGACCAAATACTTTCAGTTATGCTGAGTTGAGAGCTGCAACCGAAGATTTTAATCCTTCAAATAAGTTAGGAGAAGGAGGATATGGCCCTGTTTATAAGGGTACACTTTCTGATGGGAGAGTAGTGGCTGTGAAGCAACTTTCAGTAGCATCTCACCAAGGGAAGAGTCAATTTGTATCTGAAATTGCTACCATATCTGCTGTGCAACATCGAAATCTAGTGAAATTGTATGGATGCTGCATCGAAGGCAGCCACCGCATTTTGGTTTATGAGTATCTTGAAAACAAGAGCCTTGATCAAGCACTTTTTGGAACAAGTAACTTGCACCTTGATTGGCCTACTCGATTCAATATATTGTTGGGAACAGCAAGAGGACTTGCTTACCTTCATGAGGAGTCAAGGCCAAGGATTGTACATCGAGATGTCAAAGCGAGTAATATTTTGCTCAATGCAGAACTCTCCCCAAAAATATCAGATTTTGGACTAGCAAAGCTTTATGATGACGAGAAAACCCACATCAGCACCCGGGTTGCAGGGACAATAGGCTATTTGGCACCGGAGTATGCATTGTTTGGACATTTGACAGAGAAGGTCGATGTGTTTGGTTTTGGAGTCGTCGTTTTGGAGATCCTCAGCGGGAGACCAAACTCTTACAATAACTTGGATCCAAAAAAGATTTATCTTCTTGAATGGGTATGGACTCTACATGAAAACGACCAAACTCTGGGGCTGGTGGATCCAAGATTGACAGAGTTTGATGAAACTGAAGCAACTAGATTGATACGAGCAGCTCTCTTGTGCACGCAGGGATCACCGATGGCGAGGCCATCTATGTCACGCGTGGCTGCAATGCTCTCTGGAGACATTGACTTAGGCACTGTCATGTCGAAGCCAAGTTATTTGACAGATTATGATTTTAAAGATGTAATAACATTGTCAACAAATAGTTTTTTGGTGGGGGATGATACCCCATCAACTGCATCCAAGGATAGTAATGTTCGCCTCAACTATCAGCCGGGAGGCAGCAATGCAAGTGGTGCTAACACTCCCTGGATTGACCCTGCGCCTTCTCCTGTAAATGTCACTCAATCACTGCTCACTGGCATTAGAGTAGAAGGAAGGTGA

Protein Analysis

1037

Amino Acids

113.96

Weight (kDa)

5.84

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 158 - 268 2.5e-07 Leucine-rich repeat region
LRR_14 PF23598 183 - 290 2.8e-08 Leucine-rich repeat region
LRR_14 PF23598 274 - 343 3.1e-06 Leucine-rich repeat region
Malectin PF11721 409 - 595 2.6e-36 Malectin domain
Pkinase PF00069 681 - 886 5.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 682 - 889 1.4e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000088)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G56120 AT1G56120 AT1G56120 AT1G56130 AT1G56130 AT1G56130 AT1G56140 AT1G56145 AT1G56145 AT1G56145
fragaria_vesca FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_1g21070 FvH4_3g15410 FvH4_3g15410 FvH4_3g15430 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g15440 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g40970 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030 FvH4_3g41030
malus_domestica MD00G1023600.v1.1 MD00G1024400.v1.1 MD00G1024500.v1.1 MD00G1046700.v1.1 MD00G1047400.v1.1 MD00G1183000.v1.1 MD05G1180400.v1.1 MD05G1180500.v1.1 MD05G1257000.v1.1 MD05G1257600.v1.1 MD05G1260400.v1.1 MD05G1260700.v1.1 MD05G1260900.v1.1 MD05G1261000.v1.1 MD05G1261100.v1.1 MD15G1239100.v1.1 MD15G1239200.v1.1 MD15G1239300.v1.1 MD15G1239400.v1.1 MD15G1239500.v1.1 MD15G1240200.v1.1
prunus_persica Prupe.4G104300_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104500_v2.0.a1 Prupe.4G104700_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G104900_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G105100_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.4G136900_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1 Prupe.7G040400_v2.0.a1
pyrus_communis pycom05g20610 pycom05g23640 pycom05g23650 pycom05g23660 pycom05g23700 pycom05g23820 pycom05g23830 pycom05g23840 pycom05g23850 pycom05g23960 pycom05g23970 pycom05g23980 pycom05g24080 pycom05g24100 pycom05g24110 pycom05g24180 pycom05g24190 pycom05g24230 pycom05g24240 pycom05g24420 pycom05g24440 pycom05g24450 pycom05g24460 pycom05g24480
rosa_chinensis RchiOBHm_Chr2g0114641 RchiOBHm_Chr2g0114651 RchiOBHm_Chr2g0114711 RchiOBHm_Chr2g0114821 RchiOBHm_Chr2g0114861 RchiOBHm_Chr2g0114871 RchiOBHm_Chr2g0124231 RchiOBHm_Chr2g0142621 RchiOBHm_Chr3g0487791 RchiOBHm_Chr5g0018731 RchiOBHm_Chr5g0018761 RchiOBHm_Chr5g0018861 RchiOBHm_Chr5g0018881 RchiOBHm_Chr5g0018891 RchiOBHm_Chr5g0018901 RchiOBHm_Chr5g0018911 RchiOBHm_Chr5g0025891 RchiOBHm_Chr5g0025931 RchiOBHm_Chr5g0067281 RchiOBHm_Chr5g0073701 RchiOBHm_Chr5g0073751 RchiOBHm_Chr5g0073781 RchiOBHm_Chr5g0083701 RchiOBHm_Chr5g0083711
rosa_laevigata RLG00000018119 RLG00000018124 RLG00000018129 RLG00000018789 RLG00000032392 RLG00000032393 RLG00000032396 RLG00000032397 RLG00000032404 RLG00000032405 RLG00000032407 RLG00000032408 RLG00000032409 RLG00000032881 RLG00000032882 RLG00000036415 RLG00000036417 RLG00000037039
rosa_multiflora Rmu_co7975474.1_g000001 Rmu_co7979010.1_g000001 Rmu_co8379171.1_g000001 Rmu_co8499889.1_g000001 Rmu_sc0000370.1_g000013 Rmu_sc0000370.1_g000026 Rmu_sc0000371.1_g000027 Rmu_sc0000371.1_g000040 Rmu_sc0000563.1_g000010 Rmu_sc0000563.1_g000020 Rmu_sc0000968.1_g000029 Rmu_sc0001296.1_g000001 Rmu_sc0001296.1_g000003 Rmu_sc0001296.1_g000005 Rmu_sc0002764.1_g000042 Rmu_sc0002764.1_g000056 Rmu_sc0002968.1_g000007 Rmu_sc0003020.1_g000001 Rmu_sc0003020.1_g000002 Rmu_sc0004438.1_g000005 Rmu_sc0004501.1_g000011 Rmu_sc0004990.1_g000002 Rmu_sc0004990.1_g000022 Rmu_sc0005472.1_g000014 Rmu_sc0005839.1_g000003 Rmu_sc0005839.1_g000004 Rmu_sc0009248.1_g000007 Rmu_sc0014265.1_g000001 Rmu_sc0014538.1_g000007 Rmu_sc0016536.1_g000001 Rmu_sc0024156.1_g000001 Rmu_sc0032205.1_g000001
rosa_roxburghii Rroxscaffold_1G00007560 Rroxscaffold_1G00007610 Rroxscaffold_1G00053320 Rroxscaffold_1G00058940 Rroxscaffold_1G00058950 Rroxscaffold_1G00059000 Rroxscaffold_1G00059010 Rroxscaffold_1G00059040 Rroxscaffold_1G00073840 Rroxscaffold_1G00073870 Rroxscaffold_1G00073910 Rroxscaffold_1G00073920 Rroxscaffold_2G00103580 Rroxscaffold_2G00120060 Rroxscaffold_2G00130350 Rroxscaffold_3G00218190 Rroxscaffold_3G00237270
rosa_rugosa Rorug02G0190800.1 Rorug02G0190900 Rorug02G0190900 Rorug02G0191000 Rorug02G0191100 Rorug02G0191200 Rorug02G0249700 Rorug02G0249800 Rorug02G0249900 Rorug02G0250000 Rorug05G0046300 Rorug05G0046400 Rorug05G0046500 Rorug05G0046600 Rorug05G0089500 Rorug05G0089500 Rorug05G0089600 Rorug05G0089700 Rorug05G0092000 Rorug05G0426100 Rorug05G0485000
rosa_samantha Rh2AG249300 Rh2AG249800 Rh2CG252900 Rh2CG253200 Rh2CG254000 Rh2CG254200 Rh2DG257000 Rh2DG257100 Rh2DG257600 Rh2DG258000 Rh2DG333000 Rh2DG333100 Rh3DG318500 Rh4AG222800 Rh4DG033400 Rh5AG137400 Rh5AG137600 Rh5AG137700 Rh5AG137800 Rh5AG138100 Rh5AG138200 Rh5AG138300 Rh5AG138400 Rh5AG181400 Rh5AG181500 Rh5AG181600 Rh5AG483000 Rh5AG483600 Rh5AG537500 Rh5AG538200 Rh5BG129600 Rh5BG136800 Rh5BG136900 Rh5BG137000 Rh5CG148200 Rh5CG148400 Rh5CG197700 Rh5CG527400 Rh5CG527900 Rh5DG137200 Rh5DG137400 Rh5DG137600 Rh5DG137700 Rh5DG137800 Rh5DG137900 Rh5DG138000 Rh5DG180000 Rh5DG516100 Rh5DG516600 Rh5DG571400 Rh6DG496500
rosa_wichuraiana Rw0G007630 Rw2G019360 Rw2G019370 Rw2G019380 Rw2G019400 Rw2G019440 Rw2G019460 Rw2G024860 Rw5G012180 Rw5G012230 Rw5G012240 Rw5G012260 Rw5G016460 Rw5G016510 Rw5G044870 Rw5G044900 Rw5G044930 Rw5G044970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2082
Acc65I GGTACC 2 cut(s) 893, 1604
AccB1I GGYRCC 4 cut(s) 203, 893, 1604, 2539
AccB7I CCANNNNNTGG 2 cut(s) 551, 2710
AccI GTMKAC 2 cut(s) 502, 928
AccII CGCG 1 cut(s) 2826
AccIII TCCGGA 2 cut(s) 530, 1034
AciI CCGC 4 cut(s) 171, 1609, 2242, 2622
AclI AACGTT 1 cut(s) 1514
AcoI YGGCCR 1 cut(s) 442
AcsI RAATTY 4 cut(s) 576, 609, 1186, 1707
AcuI CTGAAG 4 cut(s) 120, 1052, 1779, 2772
AfaI GTAC 5 cut(s) 308, 895, 1606, 2089, 2400
AfiI CCNNNNNNNGG 6 cut(s) 185, 551, 1754, 2248, 2377, 2710
AflII CTTAAG 1 cut(s) 488
AflIII ACRYGT 2 cut(s) 1579, 2824
AjiI CACGTC 2 cut(s) 1262, 1580
AjnI CCWGG 4 cut(s) 1162, 1459, 1482, 3035
AjuI GAANNNNNNNTTGG 4 cut(s) 423, 455, 751, 783
AloI GAACNNNNNNTCC 2 cut(s) 526, 558
Alw21I GWGCWC 2 cut(s) 824, 2789
Alw26I GTCTC 3 cut(s) 1523, 2620, 2841
Alw44I GTGCAC 1 cut(s) 2785
AlwNI CAGNNNCTG 2 cut(s) 937, 1883
Ama87I CYCGRG 2 cut(s) 1836, 2511
Aor13HI TCCGGA 2 cut(s) 530, 1034
ApaI GGGCCC 1 cut(s) 465
ApaLI GTGCAC 1 cut(s) 2785
ApeKI GCWGC 9 cut(s) 48, 1778, 1790, 2021, 2223, 2235, 2774, 2831, 3009
ApoI RAATTY 4 cut(s) 576, 609, 1186, 1707
AseI ATTAAT 2 cut(s) 884, 1907
Asp700I GAANNNNTTC 2 cut(s) 380, 1968
Asp718I GGTACC 2 cut(s) 893, 1604
AspLEI GCGC 2 cut(s) 1548, 3052
AsuC2I CCSGG 5 cut(s) 1430, 1819, 2512, 2513, 3003
AsuHPI GGTGA 8 cut(s) 274, 617, 979, 1102, 1402, 1769, 2132, 2790
AvaI CYCGRG 2 cut(s) 1836, 2511
AvaII GGWCC 4 cut(s) 1037, 1244, 1301, 1499
BaeGI GKGCMC 2 cut(s) 465, 2789
BalI TGGCCA 1 cut(s) 444
BamHI GGATCC 2 cut(s) 2650, 2722
BanI GGYRCC 4 cut(s) 203, 893, 1604, 2539
BanII GRGCYC 2 cut(s) 465, 824
BarI GAAGNNNNNNTAC 2 cut(s) 738, 770
BbsI GAAGAC 1 cut(s) 1293
Bbv12I GWGCWC 2 cut(s) 824, 2789
BbvCI CCTCAGC 2 cut(s) 44, 2618
BbvI GCAGC 9 cut(s) 35, 1777, 1790, 2008, 2210, 2247, 2786, 2818, 3021
BceAI ACGGC 2 cut(s) 217, 1780
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 4 cut(s) 1164, 1461, 1484, 3037
BciVI GTATCC 1 cut(s) 18
BclI TGATCA 2 cut(s) 1092, 2281
BcnI CCSGG 5 cut(s) 1430, 1819, 2512, 2513, 3003
BcoDI GTCTC 3 cut(s) 1523, 2620, 2841
BfaI CTAG 5 cut(s) 1571, 1973, 2204, 2471, 2760
BfmI CTRYAG 2 cut(s) 181, 929
BfrI CTTAAG 1 cut(s) 488
BfuI GTATCC 1 cut(s) 18
BisI GCNGC 9 cut(s) 49, 1779, 1791, 2022, 2224, 2236, 2775, 2832, 3010
BlsI GCNGC 9 cut(s) 50, 1780, 1792, 2023, 2225, 2237, 2776, 2833, 3011
Bme1390I CCNGG 9 cut(s) 1164, 1430, 1461, 1484, 1819, 2512, 2513, 3003, 3037
Bme18I GGWCC 4 cut(s) 1037, 1244, 1301, 1499
BmeT110I CYCGRG 2 cut(s) 1836, 2511
BmgBI CACGTC 2 cut(s) 1262, 1580
BmiI GGNNCC 8 cut(s) 205, 391, 463, 895, 1606, 2541, 2652, 2724
BmrFI CCNGG 9 cut(s) 1164, 1430, 1461, 1484, 1819, 2512, 2513, 3003, 3037
BmrI ACTGGG 2 cut(s) 515, 1078
BmsI GCATC 4 cut(s) 2144, 2210, 2235, 2975
BmuI ACTGGG 2 cut(s) 515, 1078
BpiI GAAGAC 1 cut(s) 1293
BplI GAGNNNNNCTC 4 cut(s) 811, 843, 2763, 2795
BpmI CTGGAG 4 cut(s) 528, 554, 1163, 2865
Bpu10I CCTNAGC 2 cut(s) 44, 2618
BpuEI CTTGAG 2 cut(s) 1421, 1511
BpuMI CCSGG 5 cut(s) 1430, 1819, 2512, 2513, 3003
Bsa29I ATCGAT 1 cut(s) 369
BsaBI GATNNNNATC 1 cut(s) 2037
BsaI GGTCTC 1 cut(s) 2620
BsaWI WCCGGW 5 cut(s) 530, 1034, 1246, 1412, 2542
BsaXI ACNNNNNCTCC 4 cut(s) 526, 556, 2589, 2619
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 551, 1754, 2248, 2377, 2710
Bse118I RCCGGY 1 cut(s) 397
Bse1I ACTGG 8 cut(s) 510, 636, 895, 942, 1084, 1323, 1861, 3091
Bse3DI GCAATG 4 cut(s) 153, 1354, 2841, 3019
Bse8I GATNNNNATC 1 cut(s) 2037
BseAI TCCGGA 2 cut(s) 530, 1034
BseBI CCWGG 4 cut(s) 1164, 1461, 1484, 3037
BseCI ATCGAT 1 cut(s) 369
BseGI GGATG 6 cut(s) 465, 897, 1004, 2225, 2953, 2966
BseJI GATNNNNATC 1 cut(s) 2037
BseLI CCNNNNNNNGG 6 cut(s) 185, 551, 1754, 2248, 2377, 2710
BseMI GCAATG 4 cut(s) 153, 1354, 2841, 3019
BseMII CTCAG 7 cut(s) 58, 687, 935, 1305, 1460, 2001, 2632
BseNI ACTGG 8 cut(s) 510, 636, 895, 942, 1084, 1323, 1861, 3091
BseRI GAGGAG 3 cut(s) 814, 1945, 2393
BseSI GKGCMC 2 cut(s) 465, 2789
BseXI GCAGC 9 cut(s) 35, 1777, 1790, 2008, 2210, 2247, 2786, 2818, 3021
BseYI CCCAGC 1 cut(s) 232
Bsh1236I CGCG 1 cut(s) 2826
Bsh1285I CGRYCG 2 cut(s) 1063, 1382
BshNI GGYRCC 4 cut(s) 203, 893, 1604, 2539
BshVI ATCGAT 1 cut(s) 369
BsiEI CGRYCG 2 cut(s) 1063, 1382
BsiHKAI GWGCWC 2 cut(s) 824, 2789
BsiHKCI CYCGRG 2 cut(s) 1836, 2511
BslFI GGGAC 6 cut(s) 149, 1272, 1441, 1634, 1747, 2536
BslI CCNNNNNNNGG 6 cut(s) 185, 551, 1754, 2248, 2377, 2710
BsmAI GTCTC 3 cut(s) 1523, 2620, 2841
BsmBI CGTCTC 1 cut(s) 1523
BsmFI GGGAC 6 cut(s) 149, 1272, 1441, 1634, 1747, 2536
BsmI GAATGC 1 cut(s) 401
Bso31I GGTCTC 1 cut(s) 2620
BsoBI CYCGRG 2 cut(s) 1836, 2511
Bsp120I GGGCCC 1 cut(s) 461
Bsp1286I GDGCHC 3 cut(s) 465, 824, 2789
Bsp13I TCCGGA 2 cut(s) 530, 1034
Bsp1407I TGTACA 2 cut(s) 306, 2398
BspACI CCGC 4 cut(s) 171, 1609, 2242, 2622
BspCNI CTCAG 7 cut(s) 57, 686, 936, 1304, 1461, 2002, 2631
BspDI ATCGAT 1 cut(s) 369
BspEI TCCGGA 2 cut(s) 530, 1034
BspFNI CGCG 1 cut(s) 2826
BspHI TCATGA 1 cut(s) 2374
BspLI GGNNCC 8 cut(s) 205, 391, 463, 895, 1606, 2541, 2652, 2724
BspMAI CTGCAG 1 cut(s) 185
BspT107I GGYRCC 4 cut(s) 203, 893, 1604, 2539
BspTI CTTAAG 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 2620
BsrDI GCAATG 4 cut(s) 153, 1354, 2841, 3019
BsrFI RCCGGY 1 cut(s) 397
BsrGI TGTACA 2 cut(s) 306, 2398
BsrI ACTGG 8 cut(s) 510, 636, 895, 942, 1084, 1323, 1861, 3091
BssAI RCCGGY 1 cut(s) 397
BssNAI GTATAC 1 cut(s) 503
BssT1I CCWWGG 5 cut(s) 471, 1635, 2143, 2390, 2970
Bst1107I GTATAC 1 cut(s) 503
Bst2UI CCWGG 4 cut(s) 1164, 1461, 1484, 3037
Bst4CI ACNGT 7 cut(s) 251, 665, 1238, 1510, 1534, 1813, 2866
Bst6I CTCTTC 3 cut(s) 1047, 1164, 2144
BstAFI CTTAAG 1 cut(s) 488
BstAPI GCANNNNNTGC 1 cut(s) 3023
BstAUI TGTACA 2 cut(s) 306, 2398
BstC8I GCNNGC 3 cut(s) 300, 1901, 2789
BstENI CCTNNNNNAGG 1 cut(s) 2375
BstF5I GGATG 6 cut(s) 465, 897, 1004, 2225, 2953, 2966
BstFNI CGCG 1 cut(s) 2826
BstHHI GCGC 2 cut(s) 1548, 3052
BstMAI GTCTC 3 cut(s) 1523, 2620, 2841
BstMCI CGRYCG 2 cut(s) 1063, 1382
BstMWI GCNNNNNNNGC 7 cut(s) 45, 1056, 1552, 1787, 2232, 2241, 3023
BstNI CCWGG 4 cut(s) 1164, 1461, 1484, 3037
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 9 cut(s) 1162, 1428, 1459, 1482, 1817, 2510, 2511, 3001, 3035
BstSFI CTRYAG 2 cut(s) 181, 929
BstSLI GKGCMC 2 cut(s) 465, 2789
BstUI CGCG 1 cut(s) 2826
BstV1I GCAGC 9 cut(s) 35, 1777, 1790, 2008, 2210, 2247, 2786, 2818, 3021
BstV2I GAAGAC 1 cut(s) 1293
BstX2I RGATCY 5 cut(s) 188, 940, 2613, 2650, 2722
BstYI RGATCY 5 cut(s) 188, 940, 2613, 2650, 2722
BstZ17I GTATAC 1 cut(s) 503
Bsu15I ATCGAT 1 cut(s) 369
BsuI GTATCC 1 cut(s) 18
BsuTUI ATCGAT 1 cut(s) 369
BtrI CACGTC 2 cut(s) 1262, 1580
BtsCI GGATG 6 cut(s) 465, 897, 1004, 2225, 2953, 2966
BtsI GCAGTG 2 cut(s) 190, 3077
Cac8I GCNNGC 3 cut(s) 300, 1901, 2789
CaiI CAGNNNCTG 2 cut(s) 937, 1883
CciI TCATGA 1 cut(s) 2374
CfoI GCGC 2 cut(s) 1548, 3052
Cfr10I RCCGGY 1 cut(s) 397
Cfr9I CCCGGG 1 cut(s) 2511
ClaI ATCGAT 1 cut(s) 369
Csp6I GTAC 5 cut(s) 307, 894, 1605, 2088, 2399
CspCI CAANNNNNGTGG 4 cut(s) 1222, 1257, 3001, 3036
CviAII CATG 8 cut(s) 273, 583, 991, 1194, 1787, 2375, 2693, 2869
CviQI GTAC 5 cut(s) 307, 894, 1605, 2088, 2399
DraI TTTAAA 1 cut(s) 2905
EaeI YGGCCR 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 1047, 1164, 2144
EarI CTCTTC 3 cut(s) 1047, 1164, 2144
Ecl136II GAGCTC 1 cut(s) 822
Eco130I CCWWGG 5 cut(s) 471, 1635, 2143, 2390, 2970
Eco24I GRGCYC 2 cut(s) 465, 824
Eco31I GGTCTC 1 cut(s) 2620
Eco32I GATATC 2 cut(s) 350, 1591
Eco47I GGWCC 4 cut(s) 1037, 1244, 1301, 1499
Eco53kI GAGCTC 1 cut(s) 822
Eco57I CTGAAG 4 cut(s) 120, 1052, 1779, 2772
Eco88I CYCGRG 2 cut(s) 1836, 2511
EcoICRI GAGCTC 1 cut(s) 822
EcoNI CCTNNNNNAGG 1 cut(s) 2375
EcoO109I RGGNCCY 1 cut(s) 1982
EcoRII CCWGG 4 cut(s) 1162, 1459, 1482, 3035
EcoRV GATATC 2 cut(s) 350, 1591
EcoT14I CCWWGG 5 cut(s) 471, 1635, 2143, 2390, 2970
EcoT22I ATGCAT 1 cut(s) 2554
EcoT38I GRGCYC 2 cut(s) 465, 824
ErhI CCWWGG 5 cut(s) 471, 1635, 2143, 2390, 2970
Esp3I CGTCTC 1 cut(s) 1523
FaeI CATG 8 cut(s) 276, 586, 994, 1197, 1790, 2378, 2696, 2872
FaqI GGGAC 6 cut(s) 149, 1272, 1441, 1634, 1747, 2536
FatI CATG 8 cut(s) 272, 582, 990, 1193, 1786, 2374, 2692, 2868
FauI CCCGC 2 cut(s) 1616, 2615
FbaI TGATCA 2 cut(s) 1092, 2281
FblI GTMKAC 2 cut(s) 502, 928
Fnu4HI GCNGC 9 cut(s) 49, 1779, 1791, 2022, 2224, 2236, 2775, 2832, 3010
FokI GGATG 6 cut(s) 452, 884, 991, 2232, 2953, 2960
FriOI GRGCYC 2 cut(s) 465, 824
Fsp4HI GCNGC 9 cut(s) 49, 1779, 1791, 2022, 2224, 2236, 2775, 2832, 3010
FspBI CTAG 5 cut(s) 1571, 1973, 2204, 2471, 2760
GlaI GCGC 2 cut(s) 1547, 3051
GluI GCNGC 9 cut(s) 49, 1779, 1791, 2022, 2224, 2236, 2775, 2832, 3010
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 4 cut(s) 528, 554, 1163, 2865
HhaI GCGC 2 cut(s) 1548, 3052
Hin1II CATG 8 cut(s) 276, 586, 994, 1197, 1790, 2378, 2696, 2872
Hin6I GCGC 2 cut(s) 1546, 3050
HinP1I GCGC 2 cut(s) 1546, 3050
HincII GTYRAC 2 cut(s) 1123, 2925
HindII GTYRAC 2 cut(s) 1123, 2925
HindIII AAGCTT 1 cut(s) 2477
HphI GGTGA 8 cut(s) 274, 617, 979, 1102, 1402, 1769, 2132, 2790
Hpy166II GTNNAC 9 cut(s) 503, 929, 987, 1123, 1244, 1375, 2090, 2787, 2925
Hpy8I GTNNAC 9 cut(s) 503, 929, 987, 1123, 1244, 1375, 2090, 2787, 2925
Hpy99I CGWCG 4 cut(s) 203, 1581, 1961, 2606
HpyCH4III ACNGT 7 cut(s) 251, 665, 1238, 1510, 1534, 1813, 2866
HpyCH4IV ACGT 3 cut(s) 1261, 1514, 1579
HpyF10VI GCNNNNNNNGC 7 cut(s) 45, 1056, 1552, 1787, 2232, 2241, 3023
HpySE526I ACGT 3 cut(s) 1261, 1514, 1579
Hsp92II CATG 8 cut(s) 276, 586, 994, 1197, 1790, 2378, 2696, 2872
HspAI GCGC 2 cut(s) 1546, 3050
Kpn2I TCCGGA 2 cut(s) 530, 1034
KpnI GGTACC 2 cut(s) 897, 1608
Ksp22I TGATCA 2 cut(s) 1092, 2281
LmnI GCTCC 7 cut(s) 475, 493, 573, 819, 827, 1432, 1896
Lsp1109I GCAGC 9 cut(s) 35, 1777, 1790, 2008, 2210, 2247, 2786, 2818, 3021
LweI GCATC 4 cut(s) 2144, 2210, 2235, 2975
MaeI CTAG 5 cut(s) 1571, 1973, 2204, 2471, 2760
MaeII ACGT 3 cut(s) 1261, 1514, 1579
MaeIII GTNAC 7 cut(s) 1318, 1408, 1423, 1510, 2303, 2820, 3067
MflI RGATCY 5 cut(s) 188, 940, 2613, 2650, 2722
MhlI GDGCHC 3 cut(s) 465, 824, 2789
MlsI TGGCCA 1 cut(s) 444
MluI ACGCGT 1 cut(s) 2824
MluNI TGGCCA 1 cut(s) 444
MlyI GAGTC 6 cut(s) 606, 1283, 2161, 2390, 2607, 2680
MmeI TCCRAC 1 cut(s) 1867
Mox20I TGGCCA 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 2554
MroI TCCGGA 2 cut(s) 530, 1034
MroXI GAANNNNTTC 2 cut(s) 380, 1968
MscI TGGCCA 1 cut(s) 444
MseI TTAA 9 cut(s) 119, 489, 840, 866, 884, 1653, 1907, 2037, 2904
MslI CAYNNNNRTG 3 cut(s) 801, 2406, 2867
Msp20I TGGCCA 1 cut(s) 444
MspA1I CMGCKG 6 cut(s) 48, 64, 171, 591, 1781, 2622
MspCI CTTAAG 1 cut(s) 488
MspR9I CCNGG 9 cut(s) 1164, 1430, 1461, 1484, 1819, 2512, 2513, 3003, 3037
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 4 cut(s) 1164, 1461, 1484, 3037
MvnI CGCG 1 cut(s) 2826
MwoI GCNNNNNNNGC 7 cut(s) 45, 1056, 1552, 1787, 2232, 2241, 3023
NciI CCSGG 5 cut(s) 1430, 1819, 2512, 2513, 3003
NlaIII CATG 8 cut(s) 276, 586, 994, 1197, 1790, 2378, 2696, 2872
NlaIV GGNNCC 8 cut(s) 205, 391, 463, 895, 1606, 2541, 2652, 2724
NmuCI GTSAC 4 cut(s) 1408, 1423, 2820, 3067
NsiI ATGCAT 1 cut(s) 2554
NspI RCATGY 1 cut(s) 276
PaeR7I CTCGAG 1 cut(s) 1836
PagI TCATGA 1 cut(s) 2374
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 401
PdmI GAANNNNTTC 2 cut(s) 380, 1968
PfeI GAWTC 4 cut(s) 682, 1674, 1918, 2331
PflMI CCANNNNNTGG 2 cut(s) 551, 2710
PfoI TCCNGGA 1 cut(s) 1162
PkrI GCNGC 9 cut(s) 50, 1780, 1792, 2023, 2225, 2237, 2776, 2833, 3011
Ple19I CGATCG 2 cut(s) 1063, 1382
PleI GAGTC 6 cut(s) 605, 1283, 2160, 2389, 2606, 2680
PpsI GAGTC 6 cut(s) 605, 1283, 2160, 2389, 2606, 2680
PshBI ATTAAT 2 cut(s) 884, 1907
PsiI TTATAA 1 cut(s) 2082
Psp124BI GAGCTC 1 cut(s) 824
Psp1406I AACGTT 1 cut(s) 1514
Psp6I CCWGG 4 cut(s) 1162, 1459, 1482, 3035
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 4 cut(s) 1162, 1459, 1482, 3035
PspN4I GGNNCC 8 cut(s) 205, 391, 463, 895, 1606, 2541, 2652, 2724
PspOMI GGGCCC 1 cut(s) 461
PspXI VCTCGAGB 1 cut(s) 1836
PstI CTGCAG 1 cut(s) 185
PstNI CAGNNNCTG 2 cut(s) 937, 1883
PsuI RGATCY 5 cut(s) 188, 940, 2613, 2650, 2722
PvuI CGATCG 2 cut(s) 1063, 1382
PvuII CAGCTG 4 cut(s) 48, 64, 591, 1781
RsaI GTAC 5 cut(s) 308, 895, 1606, 2089, 2400
RsaNI GTAC 5 cut(s) 307, 894, 1605, 2088, 2399
RseI CAYNNNNRTG 3 cut(s) 801, 2406, 2867
SacI GAGCTC 1 cut(s) 824
SaqAI TTAA 9 cut(s) 119, 489, 840, 866, 884, 1653, 1907, 2037, 2904
SatI GCNGC 9 cut(s) 49, 1779, 1791, 2022, 2224, 2236, 2775, 2832, 3010
SchI GAGTC 6 cut(s) 606, 1283, 2161, 2390, 2607, 2680
ScrFI CCNGG 9 cut(s) 1164, 1430, 1461, 1484, 1819, 2512, 2513, 3003, 3037
SduI GDGCHC 3 cut(s) 465, 824, 2789
SfaNI GCATC 4 cut(s) 2144, 2210, 2235, 2975
SfcI CTRYAG 2 cut(s) 181, 929
Sfr274I CTCGAG 1 cut(s) 1836
SinI GGWCC 4 cut(s) 1037, 1244, 1301, 1499
SlaI CTCGAG 1 cut(s) 1836
SmaI CCCGGG 1 cut(s) 2513
SmiMI CAYNNNNRTG 3 cut(s) 801, 2406, 2867
SmlI CTYRAG 4 cut(s) 488, 1400, 1490, 1836
SmoI CTYRAG 4 cut(s) 488, 1400, 1490, 1836
SsiI CCGC 4 cut(s) 171, 1609, 2242, 2622
SspI AATATT 3 cut(s) 123, 1911, 2425
SspMI CTAG 5 cut(s) 1571, 1973, 2204, 2471, 2760
SstI GAGCTC 1 cut(s) 824
StyD4I CCNGG 9 cut(s) 1162, 1428, 1459, 1482, 1817, 2510, 2511, 3001, 3035
StyI CCWWGG 5 cut(s) 471, 1635, 2143, 2390, 2970
TaaI ACNGT 7 cut(s) 251, 665, 1238, 1510, 1534, 1813, 2866
TaiI ACGT 3 cut(s) 1264, 1517, 1582
TatI WGTACW 2 cut(s) 306, 2398
TfiI GAWTC 4 cut(s) 682, 1674, 1918, 2331
Tru1I TTAA 9 cut(s) 119, 489, 840, 866, 884, 1653, 1907, 2037, 2904
Tru9I TTAA 9 cut(s) 119, 489, 840, 866, 884, 1653, 1907, 2037, 2904
TseFI GTSAC 4 cut(s) 1408, 1423, 2820, 3067
TseI GCWGC 9 cut(s) 48, 1778, 1790, 2021, 2223, 2235, 2774, 2831, 3009
Tsp45I GTSAC 4 cut(s) 1408, 1423, 2820, 3067
TspGWI ACGGA 1 cut(s) 1383
TspMI CCCGGG 1 cut(s) 2511
Van91I CCANNNNNTGG 2 cut(s) 551, 2710
Vha464I CTTAAG 1 cut(s) 488
VneI GTGCAC 1 cut(s) 2785
VpaK11BI GGWCC 4 cut(s) 1037, 1244, 1301, 1499
VspI ATTAAT 2 cut(s) 884, 1907
XagI CCTNNNNNAGG 1 cut(s) 2375
XapI RAATTY 4 cut(s) 576, 609, 1186, 1707
XceI RCATGY 1 cut(s) 276
XhoI CTCGAG 1 cut(s) 1836
XmaI CCCGGG 1 cut(s) 2511
XmiI GTMKAC 2 cut(s) 502, 928
XmnI GAANNNNTTC 2 cut(s) 380, 1968
XspI CTAG 5 cut(s) 1571, 1973, 2204, 2471, 2760
Zsp2I ATGCAT 1 cut(s) 2554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.